Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| thalamic projections to visually responsive regions of parietal cortex. | thalamic efferents to visually responsive regions of parietal cortex of the cat were investigated by experimental retrograde tracing techniques. two classes of photically evoked potentials recorded from the middle suprasylvian gyrus could be distinguished on the basis of waveform and latency. type 1 responses were recorded from the clare-bishop area at the lateral border of the suprasylvian gyrus and type 2 responses were recorded from association response areas on the crown of the suprasylvian ... | 1976 | 1009447 |
| the development of the isthmo-optic nucleus. | the nucleus of origin of centrifugal fibers to the retina (the so-called isthmo-optic nucleus - ion) has been used as a model for the study of the major features of neural development, from the period of cell proliferation until after the formation of its afferent and efferent connections. 3h-thymidine autoradiography has established that in the chick cells of the ion are generated (i.e., become post-mitotic) between the middle of the 5th and the end of the 7th days of incubation. the first-form ... | 1976 | 1016847 |
| [ultrastructural studies on the permeability of the amnion-epithelium for peroxidase and ferritin. contribution on paraplacental metabolism]. | by means tracer substances (horseradish peroxidase, ferritin) the permeability of human amnion and umbilical cord (2. to 6. lunar month) has been investigated ultrastructurally. the structure of intercellular cleft permits a passiv transfer of the water and electrolytes in both ways. the findings indicated, that the passage of proteins with a molecular weight of peroxidase predominantly takes place through the intercellular clefts less by means of pinocytosis. ferritin particles can not penetrat ... | 1976 | 1020513 |
| [origin of afferent axons in the striate cortex of the albino rat: a study with horseradish peroxidase]. | 1976 | 1031505 | |
| coronary microcirculatory factors and cardiac muscle cell injury. | coronary artery ligation with or without reperfusion was carried out in wistar rats to study the role of coronary microcirculatory factors and membrane permeability alteration of cardiac muscle cell in the evolution of cardiac muscle cell injury by using the fine structural extracellular protein tracer, horseradish peroxidase (hrp). the findings were compared with those obtained in noncoronarogenic myocardial injury models following administration of norepinephrine, a pressor, and isoproterenol, ... | 1976 | 1032011 |
| the relationship between storage and secretion of specific antibody by immune lymphoid cells: ultrastructural localization of anti-peroxidase antibodies in plaque-forming cells of the rabbit popliteal lymph node. | the ultrastructure of antibody-forming cells (afc) has been studied in the lymph node cell population from rabbits locally immunized with horseradish peroxydase (po) incorporated in complete freund's adjuvant, and the kinetics of afc development followed from day 7 to day 18 after one injection of po. identification of the afc was done by local hemolysis assay, using carboxymethyl cellulose solidifying medium and po-coated sheep erythrocytes. afc were thereafter transferred by micromanipulation ... | 1976 | 1033066 |
| organization of thalamocortical auditory system in the cat studied with horseradish peroxidase. | 1976 | 1244977 | |
| peroxidase activity of alveolar macrophages. | peroxidase activity was studied in alveolar macrophages and compared to the peroxidase activity in polymorphonuclear leukocytes using cytochemical techniques. a dense reaction product for peroxidase was observed in the primary lysosomes of polymorphonuclear leukocytes, but no significant peroxidase or peroxidative enzymes could be detected in rabbit alveolar macrophages. furthermore, following vigorous phagocytosis of zymosan particles by alveolar macrophages in vitro, no peroxidase could be det ... | 1976 | 1246123 |
| the penetration of fluorescein-conjugated and electrondense tracer proteins into xenopus tadpole optic nerves following perineural injection. | the permeability of xenopus tadpole optic nerves to macromolecules was studied in order to evaluate the usefulness of this system for studying mechanisms of serum-induced cns demyelination in vivo. single injections of either horseradish peroxidase (hrp), ferritin or fluorescein-conjugated human igg were injected around the right optic nerve and tadpoles were then sacrificed between 15 min and 48 h. each of the tracers had penetrated the nerve parenchyma by 30 min. entry of hrp and ferritin occu ... | 1976 | 1247883 |
| [experience with the use of horseradish peroxidase for light and electron microscope studies of interneuronal connections]. | by the method based on a retrograde axonal transport of exogenous horseradish peroxidase (hrp), the origins of afferentation of the motor cortex of adult cats, kittens and albino rats were studied. hrp-positive neurons were found by light and electron microscopy in the somatosensory cortex (c1) of the ipsilateral hemisphere and in the portions of the cortex of the contralateral hemisphere which were symmetrical to the site of injection of hrp. the disposition of neurons, marked by hrp, in the vt ... | 1976 | 1252130 |
| simultaneous visualization by light microscopy of two pituitary hormones in a single tissue section using a combination of indirect immunohistochemical methods. | two indirect methods involving enzyme-labeled antibodies were used to demonstrate simultaneously two distinct tissue antigens in the same histologic section without a need for antigen-antibody dissociative procedures. sections of rat pituitary gland were incubated with rabbit anti-rat luteinizing hormone followed by goat anti-rabbit gamma-globulin conjugated to horseradish peroxidase. the same sections were then further incubated with monkey anti-rat growth hormone followed by goat anti-monkey g ... | 1976 | 1254941 |
| intracellular application of horseradish peroxidase and its light and electron microscopical appearance in spinocervical tract cells. | 1976 | 1260464 | |
| ultrastructural studies of mouse leucocytes showing surface binding of peroxidase-conjugated lectins. | examination in the electron microscope of mouse spleen cells treated with conjugates of wheatgerm agglutinin and horseradish peroxidase has shown the surface of myeloid cells to be heavily stained. resting lymphocytes were unstained but became positive after stimulation by phytohaemagglutinin or concanavalin a, suggesting a relationship between the presence of this surface component and the functional state of the cell and providing a further point of similarity between myeloid and lymphoblastoi ... | 1976 | 1261094 |
| afferents to the cerebellar lateral nucleus. evidence from retrograde transport of horseradish peroxidase after pressure injections through micropipettes. | hrp was injected by pressure from glass capillary micropipettes unilaterally into the lateral nucleus of rat so as to encompass the entire nucleus, but without spread into the interpositus nuclei. the cells of origin of the afferents to the lateral nucleus were studied after retrograde transport of the hrp. the reticulotegmental nucleus of the pons was labelled bilaterally and is the major source of crossed and uncrossed reticular imputs. the pontine nuclei also provide extensive crossed and unc ... | 1976 | 1262557 |
| glomerular permeability to proteins. effects of hemodynamic factors on the distribution of endogenous immunoglobulin g and exogenous catalase in the rat glomerulus. | the distribution of endogenous immunoglobulin g (igg) and exogenous catalase was delineated in the rat glomerulus under normal and abnormal hemodynamic conditions. igg was identified by an ultrastructural immunoperoxidase technique using antirat igg fab fragments conjugated to horseradish peroxidase; catalase was identified by a cytochemical reaction. when superficial glomeruli in anesthetized munich-wistar rats were rapidly fixed in situ by dripping glutaraldehyde onto the renal surface, igg an ... | 1976 | 1263444 |
| transendothelial vesicular transport of protein following compression injury to the spinal cord. | routes of vascular leakage resulting in trauma-induced edema have not been clarified. to explore the problem we followed the fate of intravascular horseradish peroxidase (hrp) after compression injury to the thoracic cord (cats). at 90 seconds and 15 minutes, hrp was confined to the gray matter, occupying perivascular spaces, unexpanded extracellular channels, and cytoplasmic compartments of injured cells. adjoining segments contained similar but lesser deposits. at 4 hours, tracer occupied the ... | 1976 | 1263445 |
| acute hypersensitivity pneumonitis in the rabbit. i. an animal model with horseradish peroxidase as antigen. | an experimental model of acute hypersensitivity pneumonitis was developed in rabbits using horseradish peroxidase as antigen. the lesion, produced by aerosol challenge with horseradish peroxidase 21 days after a single parenteral immunization with horseradish peroxidase and complete freund adjuvant, was characterized by diffuse infiltration of respiratory bronchioles and of alveolar ducts and walls with approxomately equal numbers of mononuclear cells (lymphocytes and macrophages) and polymorph ... | 1976 | 1267257 |
| immunohistochemical localization of lh-producing cells in the adenohypophysis of the japanese quail, coturnix coturnix japonica. | the cells that produce luteinizing hormone (lh) in the adenohypophysis of the japanese quail were identified immunohistochemically using anti-chicken lh serum and horseradish peroxidase-labeled goat anti-rabbit gamma globulin serum. the lh cells are localized in the caudal lobe of the pars distalis. they are elongate in shape shape and are polarized toward the sinusoids, especially in their active states. alterations in size of lh cells are directly related to changes in circulating lh levels as ... | 1976 | 1268921 |
| nmr studies of hemoproteins: ph dependence of ferric horseradish peroxidase and horse heart myoglobin. | 1976 | 1269749 | |
| the structure of lymphatic capillaries in lymph formation. | the lymphatic vascular system consists of endothelial lined vessels which begin as blind-end tubes or saccules that are located within the connective tissue areas. this system serves as a one-way drainage apparatus for the removal of diffusible substances as well as plasma proteins that escape the blood capillaries. if permitted to accumulate, these escaped components would deplete the circulatory system of its plasma colloids and disrupt the balance of forces responsible for the control of flui ... | 1976 | 1269772 |
| projections of the locus coeruleus and adjacent pontine tegmentum in the cat. | the projections of the locus coeruleus and adjacent pontine tegmentum have been studied using anatomical and physiological methods in the cat. axonal trajectories were traced using either the fink-heimer i method following electrolytic lesions, or the autoradiographic method after injection of tritiated proline into the nucleus. results with both methods were similar. axons of locus noeruleus neurons ascended ipsilaterally through the mesencephalon lateral to the medial longitudinal fasiculus, v ... | 1976 | 1270609 |
| convergent thalamic and mesencephalic projections to the anterior medial cortex in the rat. | small microelectrophoretic deposits of horseradish-peroxidase (hrp) were placed at various loci within the gray matter of the rat's anterior medial cortex. a comparison of the patterns of retrograde cell-labeling charted in 26 such cases confirmed earlier findings in fiber-degeneration studies according to which the respective thalamocortical projections of the mediodorsal (md) and anteromedial nucleus (am) overlap each other over a wide region of the anterior medial cortex. this region of thala ... | 1976 | 1270614 |
| the olivocerebellar projection in the cat as studied with the method of retrograde axonal transport of horseradish peroxidase. ii. the projection to the uvula. | following injections of small quantities of horseradish peroxidase (hrp) suspension in the uvula of the cat, the distribution of labeled cells in the inferior olive has been mapped. the findings confirm the conclusion made on the basis of studies of retrograde cell loss in the olive following ablations of the uvula (brodal, '40b) that two small olivary subdivisions, the nucleus beta and the dorsomedial cell column project heavily to the uvula. in addition the hrp-study shows that the uvula recei ... | 1976 | 1270615 |
| scanning electron microscopy of the subarachnoid space in the dog. iv. subarachnoid macrophages. | young dogs of both sexes were used in this study. transmission and scanning electron microscopy were utilized for the examination of the spinal cord and choroid plexus with emphasis on the study of free cells. these procedures were modified so that, in certain cases, the same cells observed in scanning electron microscopy could be analyzed internally by transmission electron microscopy. one half of the animals were injected under anesthesia with horseradish peroxidase for observation of phagocyt ... | 1976 | 1270624 |
| development of an enzyme immunoassay for human placental lactogen using labelled antibodies. | an immunoassay for the determination of human placental lactogen (hpl) has been developed using specific antibodies labelled with horseradish peroxidase. a method is presented in which the enzyme : antibody conjugate is purified with an antigenic solid phase to ensure the production of conjugates with intact immunological activity. serum components interfere with the assay but this problem can only be avoided at the expense of a precise and practical assay for the determination of hpl. | 1976 | 1270811 |
| permeability alteration of sarcolemmal membrane in catecholamine-induced cardiac muscle cell injury. in vivo studies with fine structural diffusion tracer horse radish peroxidase. | cardiac muscle cell injury was produced in male sprague-dawley rats weighing 300 to 450 gm. with catecholamines, norepinephrine, and isoproterenol; sarcolemmal membrane alteration was tested in vivo using the extracellular macromolecular tracer, horseradish peroxidase. norepinephrine was administered in continuous intravenous infusion in a dose of 4 to 6 mug. per 100 gm. of body weight per minute, whereas isoproterenol was given as a single subcutaneous injection in a dose of 8.5 mg. per 100 gm. ... | 1976 | 1271749 |
| endocytosis by vascular smooth muscle cells in vivo and in vitro. roles or vesicles and lysosomes. | overloading of lysosomes of smooth muscle cells with excess substrate may be a key event in the development of hypertensive and atherosclerotic vascular disease. cellular uptake of materials and its relation to lysosomal function were studied by ultrastructural cytochemistry in aortic smooth muscle cells grown in vitro and in the intact animal. injection of horseradish peroxidase (hrp) into hypertensive rats resulted in rapid insudation of the material into the environs of medial smooth muscle c ... | 1976 | 1275059 |
| direct projections from hypothalamus to hippocampus in the rat demonstrated by retrograde transport of horseradish peroxidase. | 1976 | 1276883 | |
| an improved technique for the microinjection of horseradish peroxidase. | 1976 | 1276885 | |
| changes in the distribution of the dentate gyrus associational system following unilateral or bilateral entorhinal lesions in the adult rat. | the distribution of the dentate gyrus associational system was analyzed in naive adult rats and in those with either unilateral or bilateral lesions of the entorhinal cortex. horseradish peroxidase histochemistry was used to trace the origin and course of this intrinsic fiber system. the fibers originated in the ca3-4 pyramidal cell field, apparently medial to the origin of the schaffer collateral system, and followed a trajectory which was essentially identical to that described for this system ... | 1976 | 1276951 |
| ultrastructure and permeability of lymph node microvasculature in the mouse. | the microvasculature of lymph nodes and peyer's patches consists of arterioles, capillaries and venules. the postcapillary segment comprises high-endothelial venules (he venules) as well as ordinary venules. in order to study the ultrastructure of the microvasculature, particularly with respect to the nature of intercellular junctions, lanthanum and ruthenium red were used as tracers. furthermore, to evaluate the permeability properties of the different segments of the microvasculature, intraven ... | 1976 | 1277282 |
| cooperative binding of concanavalin a to thymocytes at 4 degrees c and micro-redistribution of concanavalin a receptors. | the mode of binding of 125i-labelled concanavalin a and succinyl-concanavalin a to rat thymocytes at 4 degrees c was investigated. simultaneously, the free binding sites of the cell-bound lectin molecules were quantified by horseradish peroxidase binding. concanavalin a showed cooperative binding while succinyl-concanavalin a did not. the number of molecules of concanavalin a bound to the cell surface when it was saturated was twice the number of molecules of succinyl-concanavalin a. we interpre ... | 1976 | 1278183 |
| uptake of horseradish peroxidase (hrp) by the endothelium of the short-term stored rabbit cornea. | in vitro study of the uptake of horseradish peroxidase (hrp) by the corneal endothelium was carried out on rabbit corneae which were stored in a preserving medium for various periods. the stored corneae were incubated in hrp solution with or without adenosine triphosphate (atp). the number of pinocytotic vesicles decreased as the storage time was prolonged. addition of atp to the hrp solution led to an increase in the pinocytotic vesicles in all storage periods. the pinocytosis in the 12-day sto ... | 1976 | 1083694 |
| interdigitating reticulum cells in the popliteal lymph node of the rat. an ultrastructural and cytochemical study. | electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (idc) in the popliteal lymph node of the rat. the morphological features of the idc of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. this is considered to be an argument in favour of the common origin of idc's and macrophages. ultrahistochemical studies with horseradish peroxidase (hrp) reveal no phagocytotic capacity of i ... | 1976 | 1084780 |
| in vitro studies on the selective binding of igg from different species to tissue sections of the bovine mammary gland. | tissue sections of the mammary gland of cattle provide a sensitive and reproducible model for studying the initial events of the selective transport of bovine iggs by the acinar epithelium (ae). mammary tissue was reacted with purified antibody to horseradish peroxidase (hrp) and exposed to hrp. the sites of peroxidase activity were revealed cytochemically. the highly selective binding of iggs by ae of the colostrum-forming gland but never of the lactating gland was most notable, whereas igg1 an ... | 1975 | 11993319 |
| retrograde axonal transport of horseradish peroxidase from spinal cord to brain stem cell groups in the cat. | 1975 | 19604744 | |
| stimulation-induced uptake of horseradish peroxidase by rat cortical synapses. | 1975 | 19604745 | |
| labelling of cells in the medulla oblongata and the spinal cord of the monkey after injections of horseradish peroxidase in the thalamus. | injections of horseradish peroxidase were performed in macaque monkeys in the intralaminar and the ventroposterior thalamic region, respectively. following injection in the intralaminar region labelled cells were present in the marginal zone both in the spinal trigeminal nuclues and in the spinal dorsal horn. the same areas contained labelled cells after injections in the ventroposterior thalamic region, but in such experiments labelled cells were also present in the lamina iv-v region of the sp ... | 1975 | 19604756 |
| the reaction of coumarins with horseradish peroxidase. | the peroxidase catalyzed oxidation of indole-3-acetate is inhibited by naturally occurring coumarins such as scopoletin. this inhibition is due to the preferential reactivity of the coumarins with the peroxidase compounds i, ii, and iii. in view of the possible growth regulatory role of coumarins in plants, the mechanism of oxidation of scopoletin by horse-radish peroxidase has been investigated.peroxidase catalyzed coumarin oxidation requires either an electron donor and molecular oxygen or hyd ... | 1975 | 16659024 |
| the isozymic similarity of indoleacetic acid oxidase to peroxidase in birch and horseradish. | the relationship of indoleacetic acid oxidase activity to peroxidase activity is complicated by numerous multiple forms of this enzyme system. it is not known if all isozymes of this complex system contain both types of activity. isozyme analysis of commercial horseradish peroxidase and leaf extracts of yellow birch (betula alleghaniensis) by isoelectric focusing in polyacrylamide gels was used to examine this problem. horseradish and birch exhibited 20 and 13 peroxidase isozymes, respectively, ... | 1975 | 16659371 |
| nuclear magnetic resonance studies of hemoproteins. binding of aromatic donor molecules to horseradish peroxidase. | 1975 | 429320 | |
| [flavonols and flavones of vegetables. v. flavonols and flavones of root vegetables (author's transl)]. | root vegetables contain flavon(ol) glycosides in tracers up to small amounts, while the level of their leaves are in part considerable (to more than 1 g/kg, calculated as aglycon). radish, rutabagas, scorzoneras, and beets contain less than 1 mg/kg kaempferol and/or quercetin; carrots less than 1 mg/kg apigenin and luteolin; celery roots ca. 75 mg apigenin/kg and 14 mg luteolin/kg; horseradish about 20 mg kaempferol/kg and small radish 1-10 mg kaempferol/kg, whereby all these flavones and flavon ... | 1975 | 1224798 |
| the origin of the annulo-olivary tract with notes on other mesencephalo-olivary pathways. a study by the horseradish peroxidase method. | 1975 | 1182508 | |
| the correlation of the time of origin of neurons with their axonal projection: the combined use of [3h]thymidine autoradiography and horseradish peroxidase histochemistry. | 1975 | 1182549 | |
| anti-horseradish peroxidase associated with tubular structures in the endoplasmic reticulum of plasma cells in stimulated lymph nodes of mice. | tubular structures are described in dilated cisternae of the endoplasmic reticulum in plasma cells of lymph nodes stimulated with horseradish peroxidase (hrp). a positive reaction for anti-hrp antibody suggests that they are formed of immunoglobulins or are at least closely associated with them. | 1975 | 1199967 |
| heme-linked protonation of hcn, co, no and o2 complexes of reduced horseradish peroxidases. | 1975 | 971 | |
| proton magnetic resonance studies of peroxidases from turnip and horseradish. | proton nmr spectra at 270 mhz have been measured for horseradish peroxidase and turnip peroxidase isoenzymes (p1, p2, p3 and p7) in both their high spin ferric native states and as the low spin ferric cyanide complexes. resonances of amino acids near the heme have been identified and used to investigate variations in the structure of the heme crevice amongst the enzymes. ligand proton resonances have been resolved in spectra of the cyanide complexes of the peroxidases and these provide informati ... | 1975 | 172144 |
| the retrograde transport of horseradish peroxidase from the developing limb of the chick embryo. | chick embryos ranging in age from 4.0 to 18 days of incubation and 1-2-day-old hatchlings received injections of hrp solutions directly into the leg musculature. after survival periods of from 5 to 25 h motoneurons and cells in the spinal sensory ganglia were found to be stained with the hrp reaction product. it was found that the first appearance of a positive hrp reaction coincided with the time when nerve processes are first detected in the limb-bud by silver techniques (i.e. at 4.5 days of i ... | 1975 | 1182520 |
| proceedings: spectroscopic and nmr studies on the binding of substrates to horseradish peroxidase. | 1975 | 1205735 | |
| proceedings: modifications of horseradish peroxidase with aromatic compounds. | 1975 | 1205733 | |
| ectopic neurons and aberrant connections during neural development. | during the normal development of the nucleus of origin of centrifugal fibers to the chick retina (the so-called isthmo-optic nucleus) a significant number of neurons are misrouted during their migration from the neural epithelium in which they are generated. like the cells in the isthmo-optic nucleus, these ectopic neurons can be identified by the retrograde transport of horseradish peroxidase (donor: hydrogen peroxide oxidoreductase, ec 1.11.1.7) injected into the eye. between 500 and 600 such ... | 1975 | 1060126 |
| the pinocytic rate of activated macrophages. | peritoneal macrophages from mice injected 4 days previously with brewer's thioglycollate medium have a pinocytic rate, in culture, of 190 ng horseradish peroxidase (hrp)/100 mug cell protein/h, compared to the rate of resident peritoneal cells of 53 ng hrp/100 mug cell protein/h. mice injected with endotoxin or with only certain of the components of the brewer's medium show an intermediate level of stimulation. the rate of unstimulated, endotoxin-stimulated, or thioglycollate-stimulated cells sh ... | 1975 | 53258 |
| the haem-enviroment in horseradish peroxidase as seen by proton magnetic relaxation. | 1975 | 1191283 | |
| circulation and turnover of synaptic vesicle membrane in cultured fetal mammalian spinal cord neurons. | intact neurons in cultures of fetal rodent spinal cord explants show stimulation-dependent uptake of horseradish peroxidase (hrp) into many small vesicles and occasional tubules and multivesicular bodies (mvb) at presynaptic terminals. presynaptic terminals were allowed to take up hrp during 1 h of strychnine-enhanced stimulation of synaptic transmitter release and then "chased" in tracer-free medium either with strychnine or with 10 mm mg++ which depresses transmitter release. tracer-containing ... | 1975 | 1176531 |
| a dual marking technique for microelectrode tracks and localization recording sites. | this paper describes techniques for marking both microelectrode tracks and exact recording loci using a combination of fast green dye and horseradish peroxidase (hrp). the procedure involves coating the exterior of hrp filled microelectrodes with fast green dye in order to identify electrode tracks, and ejecting hrp from the electrode to mark recording loci. rapid, multiple marks can be made with this technique without harming the recording capabilities of the micropipette. | 1975 | 51724 |
| similarities and differences of five peroxidases from turnip and horseradish. peptide mapping studies on glycoproteins. | four isoperoxidases of turnip root and isoperoxidase c of horseradish root were digested with trypsin, and their peptide maps, prepared by high-voltage paper electrophoresis, were compared. all five tryptic digests were completely soluble at ph 8. the maps were developed with a variety of general and specific reagents: ninhydrin, histidine, tyrosine, tryptophan and arginine reagents. cystine peptides and cysteic acid derivatives have also been characterized. all detected half-cystine residues se ... | 1975 | 1175650 |
| an electron microscopic study on the blood-optic nerve and fluid-optic nerve barrier. | utilizing horseradish peroxidase as a tracer, electron microscopic studies were done on the blood-optic nerve and fluid-optic nerve barrier to the peroxidase diffusion. following intravenous injection the peroxidase was observed to fill the lumen of the capillaries of the laminar, prelaminar and orbital portions of the optic nerve but there was no penetratation of the capillary walls. the obstruction of the tracer diffusion out of capillary walls was attributed to the tight junctions between the ... | 1975 | 1082256 |
| oxidase-peroxidase enzymes of datura innoxia. oxidation of formylphenylacetic acid ethyl ester. | an enzyme system from datura innoxia roots oxidizing formylphenylacetic acid ethyl ester was purified 38-fold by conventional methods such as (nh4)2so4 fractionation, negative adsorption on alumina cy gel and chromatography on deae-cellulose. the purified enzyme was shown to catalyse the stoicheiometric oxidation of formylphenylacetic acid ethyl ester to benzoylformic acid ethyl ester and formic acid, utilizing molecular o2. substrate analogues such as phenylacetaldehyde and phenylpyruvate were ... | 1975 | 997 |
| [microenvironment of enzymes as 1 of the factors determining enzyme stability. stabilization of soluble and immobilized horseradish peroxidase]. | 1975 | 223 | |
| the central cervical nucleus - a source of spinocerebellar fibres, demonstrated by retrograde transport of horseradish peroxidase. | injections of horseradish peroxidase in the cerebellum of kittens resulted in labelling of cells in the central cervical nucleus (ccn) in the upper cervical spinal cord. the largest number of labelled ccn neurones was found after injections including the anterior part of the anterior lobe of the cerebellum. in this neuronal system horseradish peroxidase was transported at a maximal velocity of about 150 mm/day. | 1975 | 19604757 |
| preparation and properties of matrix-supported horseradish peroxidase. | a new method of enzyme immobilization has been described using poly(4-methacryloxybenzoic acid) as the carrier. activation of the polymer, prior to enzyme attachment, was achieved with n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. the enzyme coupling step proceeded through nucleophilic attack by the protein on a mixed carbonic anhydride. the degree of polymer activation was determined by analysis for quinoline, a by-product of the reaction. the polymer-enzyme complex was compared to the enzyme ... | 1975 | 241471 |
| ultrastructural localization of in vivo-bound complement in bullous pemphigoid. | antihuman complement component c3 labeled with horseradish peroxidase was used to reveal the ultrastructural localization of complement in two cases of bullous pemphigoid. the complement deposits were shown to be exclusively located in the space between the plasma membrane of the basal cells and the basal lamina. this corresponds exactly to the ultrastructural localization of immunoglobulins in bullous pemphigoid. | 1975 | 50388 |
| an efferent connection of the solitary cells of meynert. a study with horseradish peroxidase in the marmoset callithrix. | 1975 | 809097 | |
| localization of alpha-d-glucosyl and alpha-d-mannosyl groups of mucosubstances with concanavalin a and horseradish peroxidase. | concanavalin a is a lectin which is known to bind specifically to alpha-d-glucosyl and alpha-d-mannosyl groups in the mucosubstances of mammalian tissues. the lectin molecule is bivalent; after its attachment to mucosubstances present in a histological specimen it can also bind horseradish peroxidase, a mannose-containing glycoprotein. the attached peroxidase may then be visualized by virtue of its histochemically demonstrable enzymatic activity. other investigators have utilized this principle ... | 1975 | 52638 |
| photochemical reactions of horseradish peroxidase compounds i and ii at room temperature and 13 degrees k. | some photochemical reactions of horseradish peroxidase compounds i and ii (hrp-i and hrp-ii, respectively) have been studied by electronic absorption spectroscopy over the temperature range 297 degrees k-10 degrees k. in glassy matrices below 80 degrees k hrp-i is rapidly converted to hrp-ii when irradiated with low power white light. the native enzyme and hrp-ii are not photochemically active at these temperatures with low power irradiation. at room temperature the spontaneous decay of both hrp ... | 1975 | 1148197 |
| effects of 2,4-substituents of deuteropheme upon redox potentials of horseradish peroxidases. | 1975 | 239639 | |
| epr studies on compound i of horseradish peroxidase. | compound i of horseradish peroxidase (donor: hydrogen-peroxide oxidoreductase ec 1.11.1.7) was studied by epr at low temperatures. an asymmetric signal was found, about 15 gauss wide and with a g-value of 1.995, which could be detected only at temperatures below 20 k and which had an intensity corresponding to about 1% of the heme content. in a titration with h2o2, the signal intensity was proportional to the concentration of compound i, reaching a maximum when equivalent amounts of h2o2 were ad ... | 1975 | 167831 |
| distribution of ultrastructural tracers in crustacean axons. | ruthenium red and horseradish peroxidase were used to compare the uptake of exogenous molecules into crayfish motor axons and their sheaths in severed and intact peripheral nerves. both tracers penetrated the axonal sheath and were subsequently seen lining small vesicles and tubules in the axoplasm. tracer appeared to enter the axon via pinocytotic vesicles. there were no perceptible quantitative or qualitative differences in ruthenium red uptake between intact and severed axons. however, counts ... | 1975 | 1133229 |
| the ultrastructure of normal and glycerol treated muscle in the ghost crab, ocypode cursor. | the ultrastructure of normal and glycerol treated fibers of the closer muscle of the ghost crab, ocypode cursor, was studiedmthe muscle is composed of presumably phasic (short sarcomeres) and tonic (long sarcomeres) fibers, the latter greatly predominating. horseradish peroxidase (hrp) was used as an extracellular tracer to delineate the tubular system (ts), and to determine to what extent this system becomes detached from the extracellular space as a result of glycerol treatment. sarcolemmal cl ... | 1975 | 1149103 |
| titration study of guaiacol oxidation by horseradish peroxidase. | titration of guaiacol by hydrogen peroxide in the presence of a catalytic amount of horseradish peroxidase shows that the reduction of hydrogen peroxide proceeds by the abstraction of two electrons from a guaiacol molecule. in the same way, it can be demonstrated that 0.5 mol of guaiacol can reduce, at low temperature, 1 mol of peroxidase compound i to compound ii. moreover, the reaction between equal amounts of compound i and guaiacol at low temperature produces the native enzyme. a reaction sc ... | 1975 | 1139404 |
| the results of freezing and dehydration of horseradish peroxidase. | 1975 | 1132241 | |
| the horseradish peroxidase technique applied to the teleostean nervous system. | 1975 | 50115 | |
| absorption and transport of ferritin and exogenous horseradish peroxidase in the opisthonephric kidney of the sea lamprey. i. the renal corpuscle. | 1975 | 1131752 | |
| the effects of prolonged repetitive stimulation in hemicholinium on the frog neuromuscular junction. | 1. cutaneous pectoris nerve-muscle preparations from the frog were stimulated for prolonged periods in solutions with curare alone, curare and hemicholinium no. 3 (hc-3), or curare and glucose plus choline. end-plate potentials (e.p.p.s) and miniature end-plate potentials (m.e.p.p.s) were recorded intracellularly. black widow spider venom (bwsv) was applied to determine the degree of depletion of the transmitter stores. 2. the ultrastructure of the neuromuscular junctions was studied in the elec ... | 1975 | 1079538 |
| a kinetic study of the reaction of horseradish peroxidase with hydrogen peroxide. | a kinetic study has been carried out over the ph range of 2.63-9.37 for the reaction of horseradish peroxidase with hydrogen peroxide to form compound i of th;e enzyme. analysis of the results, indicates that there are two kinetic influencing, ionizable groups on the enzyme with pka values of 3.2 and 3.9. protonation of these groups results in a decrease in the rate of reaction of the enzyme with h2o2. a previous study of the kinetics of cyanide binding to horseradish peroxidase (ellis, w.d. & d ... | 1975 | 237615 |
| histological evidence for direct connections between the optic lobes of the cockroach leucophaea maderae. | heretofore, descriptions of direct interconnections between insect optic lobes have been based on histological examinations of normal brains or on inference from electrophysiological or behavioral data. we present here what we believe to be the first demonstration of such monosynaptic connections by techniques of experimental neuroanatomy. twenty-four to 39 h after extirpation of the left optic lobe, degenerating axons and axon terminals, as silvered by a modified nauta technique, were abundant ... | 1975 | 47256 |
| anti-horseradish peroxidase associated with golgi complex of antibody-forming cells. | observations are reported on the localization of anti-horseradish peroxidase in antibody-forming cells in popliteal lymph nodes following a single injection of antigen. reaction product, marking the site of antibody, was observed not only in the ergastoplasm and cisternae of the golgi complex, but also in globules associated with the golgi complex. | 1975 | 1171612 |
| fractionation of horseradish peroxidase by preparative isoelectric focusing, gel chromatography and ion-exchange chromatography. | horseradish peroxidase has been fractionated by preparative isoelectric focusing in a density gradient and in a layer of granulated gel using ph-3-10 and narrow-ph-range carrier ampholytes at different total enzyme loads. the resolution of peroxidase isoenzymes in preparative-layer isoelectric focusing was comparable to that obtained by analytical thin-layer isoelectric focusing. isoelectrically homogeneous isoenzymes could be isolated with good recovery in a single fractionation step. despite t ... | 1975 | 240683 |
| horseradish peroxidase. xix. a photochemical reaction of compound i at 5 degrees k. | 1975 | 1125021 | |
| oxidative turnover of auxins in relation to the onset of ripening in bartlett pear. | pears (pyrus communis var. bartlett) kept in 100% o(2) showed an increase in the rate of softening, chlorophyll degradation, and ethylene evolution. the o(2) application could overcome, in part, the inhibition of ripening by 1 mm indoleacetic acid. ripening of pears was also accelerated by the application of solutions containing indoleacetic acid-oxidation products, obtained by an overnight incubation of 0.1 and 1 mm indoleacetic acid with traces of h(2)o(2) and horseradish peroxidase. although ... | 1975 | 16659106 |
| effect of 2450 mhz microwave radiation on horseradish peroxidase. | 1975 | 1041592 | |
| optical, nmr and epr properties of horseradish peroxidase and its donor complexes. | 1975 | 164382 | |
| synthesis of antibody and immunoglobulins without detectable antibody function in cells responding to horseradish peroxidase. | the kinetics of immunoglobulin-synthesizing cells (ifc) and antibody-forming cells (afc) are compared in the popliteal nodes of mice challenged, in the hind footpads, with horseradish peroxidase (po) in freund's complete adjuvant. a rise in the number of ifc without antibody function precedes the appearance of afc. ifc reach peak numbers 7 days before afc. control experiments show that the majority of ifc without antibody function are responding to po and not to adjuvant. kinetic and radioautogr ... | 1975 | 1168220 |
| nuclear magnetic resonance evidence for the absence of iron coordinated water in horseradish peroxidase. | 1975 | 234224 | |
| ethylene production by cress roots and excised cress root segments and its inhibition by 3,5-diiodo-4-hydroxybenzoic acid. | 3,5-diiodo-4-hydroxybenzoic acid (dihb) has been shown to exert an inhibitory effect on the formation of ethylene by the roots of intact cress lepidium sativum seedlings in light, and by excised cress root segments. adding iaa to the culture solution greatly promoted ethylene production, which was suppressed by dihb. the findings together with results obtained with dinitrophenol (dnp), l-methionine and l-ethionine and also the horseradish peroxidase/methional system of yang are discussed.the res ... | 1975 | 24430221 |
| [comparison of the activities of free and carrier fixed horseradish peroxidase]. | commerical horseradish-peroxidase was covalently bound to brcn-activated sepharose. the activity parameters vmax and km were determined by the leukomalachite green reaction. compared with the soluble enzyme, the immobilized pod has a relative residual activity of 22%. dimethylsulfoxide and formamide were found to diminish the enzymatic activity in a concentration-dependent manner. the activity of the free enzyme in aqueous formamide solution (10%) is reduced by 43%, that of the insolubilized enz ... | 1975 | 1199630 |
| kinetic evidence of horseradish peroxidase oxidation by compound i. | the kinetics of compound ii formation, obtained upon mixing a highly purified horseradish peroxidase and hydrogen peroxide, was spectrophotometrically studied at three wavelengths in the absence of an added reducing agent. our experiments confirm george's finding that more than one mole of compound ii is formed per mole of hydrogen peroxide added. the new mechanism that we propose, contrary to the mechanism of george, is only valid when compound ii is obtained in the absence of an added donor. m ... | 1975 | 1148326 |
| absorption and transport of ferritin and exogenous horseradish peroxidase in the opisthonephric kidney of the sey lamprey ii. the tubular nephron. | the tubular nephrons in the opisthonephric kidney of the sea lamprey, petromyzon marinus l., were demonstrated to absorb and transport exogenous protein. the proximal convoluted segment is the major site of protein absorption from the tubular lumen and the process involved is similar to that described for the proximal convoluted segment in the kidneys of other vertebrates. the lateral intercellular spaces are a possible channel for the transport of intact proteins in this segment. the cilated ne ... | 1975 | 1131856 |
| a general method for the introduction of enzymes, by means of immunoglobulin-coated liposomes, into lysosomes of deficient cells. | phagocytes of the smooth dogfish (mustelus canis) contain no endogenous peroxidase within their lysosomes and constitute models for cells genetically deficient in lysosomal enzymes such as myeloperoxidase. we have obtained uptake of over 50% of exogenous horseradish peroxidase, provided the enzyme is exhibited to cells after incorporation into liposomes coated with heat-aggregated (62 degrees, 10 min), isologous igm. trapping of horseradish peroxidase (ec 1.11.1.7) by liposomes was established b ... | 1975 | 1054517 |
| enzymatic activation and trapping of luminol-substituted peptides and proteins. a possible means of amplifying the cytotoxicity of anti-tumor antibodies. | glutathione and glucose oxidase (ec 1.1.3.4) conjugates containing covalently bound luminol were prepared as prototypes for peptides and proteins with latent, enzyme-activatable chemical reactivity. in the presence of small quantities of activated horseradish peroxidase, conjugated luminol molecules were oxidized to unstable free radicals which reacted rapidly with soluble proteins and cells. these observations are of interest in regard to possible sequential localization reactions in which a fe ... | 1975 | 47175 |
| junctional complexes in the preimplantation rabbit embryo. | the morphology and development of junctional complexes between blastomeres of the preimplantation rabbit embryo were investigated using several approaches. electron microscopic examination of embryos stained en bloc with uranyl acetate, and the study of junction permeability using horseradish peroxidase and lanthanum nitrate provided information on structure, intermembrane spacing and permeability of the junctional complexes. in addition, the freeze fracture technique was used with day 5 and day ... | 1975 | 45878 |
| localization of immunoreactive androgen in testicular tissue. | immunohistochemical localization of androgen in the testes of both rat and squirrel monkey has been performed using an antiserum specific to testosterone and certain related androgens. the bound antibodies were visualized by coupling with antigammaglobulin labeled either with fluorescein isothiocyanate or with a horseradish peroxidase-diaminobenzidine technique. the highest concentration of androgen was found in the interstitial tissue but a significant amount was also present in the seminiferou ... | 1975 | 45910 |
| thalamic afferents to areas 17, 18 and 19 of cat cortex traced with horseradish peroxidase. | 1975 | 46157 | |
| retrograde axonal transport of horseradish peroxidase in sensory nerves and ganglion cells of the rat. | 1975 | 46159 | |
| retrograde intraaxonal transport of horseradish peroxidase in retinal ganglion cells of the chick. | 1975 | 46164 | |
| the orthograde transport of horseradish peroxidase in the visual system. | 1975 | 46168 | |
| a comparison of anterograde and retrograde axonal transport of horseradish peroxidase in the connections of the mammillary nuclei in the rat. | 1975 | 46171 | |
| electron microscopic observations of horseradish peroxidase transported from the caudoputamen to the substantia nigra in the rat: possible involvement of the agranular reticulum. | the intracellular distribution of horeseradish peroxidase (hrp) transported intraaxonally from the caudoputaminal complex to the substantia nigra has been examined with the electron microscope. the reciprocal axonal connections between the caudoputamen and the substantia nigra permitted observation not only of hrp transported retrogradely from axons and axon terminals in the caudoputamen to the cell bodies of origin in the pars compacta of the substantia nigra, but also provided information sugg ... | 1975 | 46174 |
| corticothalamic neurons and thalamocortical terminal fields: an investigation in rat using horseradish peroxidase and autoradiography. | subsequent to thalamic injections in rats of horseradish peroxidase (hrp) alone or hrp and [3h]leucine in combination, the cells of origin of the corticothalamic projections and the terminal fields of the thalamocortical projections were identified. hrp-labeled corticothalamic neurons were uniformly found in layers v and vi. they were medium to small in size and always pyramidal in shape with the larger neurons being found in layer v. on the other hand, 3 different patterns for the distribution ... | 1975 | 46175 |
| use of peroxidatic-enzyme staining to enhance resolution of cultured mammalian cells under phase microscopy. | staining of glutaraldehyde-fixed mammalian cells with peroxidatic enzymes (horseradish peroxidase or horse heart cytochrome c) greatly enhances resolution of their structure under phase microscopy. the topography of cell processes and regions of intercellular contact and overlapping is resolved precisely, even in dense cultures mounted in media which ordinarily do not permit clear demonstration of these areas. the technique is therefore a useful aid to the study of cultured cells with phase opti ... | 1975 | 46631 |