Publications

peroxidase-antiperoxidase complex. binding by mallory bodies in unfixed tissue.	an antigen-antibody complex of horseradish peroxidase, peroxidase-antiperoxidase (pap), was found to selectively bind to mallory bodies (mbs). specific pap binding to mbs was consistently demonstrated in liver sections from 14 patients with alcoholic cirrhosis and from one patient with hepatoma. mallory bodies in isolated fractions also bound pap. no structures stained with pap in six control sections. the pap-binding method is useful in the identification of mbs in situ and in isolated fraction ...	1976	989279
the use of tritiated horseradish peroxidase for defining neuronal pathways: a new application.		1976	990928
the precise localization of nigral afferents in the rat as determined by a retrograde tracing technique.	afferent innervation of the rat substantia nigra (sn) was studied by the retrograde horseradish peroxidase (hrp) method. high concentrations of hrp were deposited in discrete subregions of the sn by means of a microiontophoretic delivery system. using this technique it was possible to demonstrate that the caudatonigral projection system is arranged topographically; all portions of the caudate-putamen except for a central medial core were found to contain hrp positive cells, indicative of retrogr ...	1976	990939
axonal projections of spinal interneurones excited by group i afferents in the cat, revealed by intracellular staining with horseradish peroxidase.		1976	990947
intracellular staining of purkinje cells and their axons with horseradish peroxidase.		1976	990950
lymphocyte emigration from high endothelial venules in rat lymph nodes.	sequential events during lymphocyte emigration from high endothelial venuses (hev) were studied by scanning and transmission electron microscopy combined with regional perfusion techniques. the results indicate that blood lymphocytes selectively adhere to hev surfaces through microvilli which attach to shallow pits on the luminal surfaces of high endothelial cells. these intercellular contact points resist hydrodynamic and osmotic shearing forces, but can be disrupted by treatments which remove ...	1976	992709
pinocytic rates of macrophages from mice immunized against toxoplasma gondii and macrophages stimulated to inhibit toxoplasma in vitro.	the rate of pinocytosis by macrophages when measured by uptake of horseradish peroxidase was significantly reduced during toxoplasma infection of the cells in vitro when the macrophages were from toxoplasma-immune mice and when control cells were stimulated in vitro to inhibit toxoplasma multiplication. there was, however, no direct correlation between reduced pinocytosis in this model and inhibition or enhancement of toxoplasma multiplication. we conclude that a reduced pinocytic rate is a feat ...	1976	992866
a histochemical and morphologic study of postobstructive diuresis in the rat.	renal functional abnormalities constituting the syndrome of postobstructive diuresis imply both altered tubular and glomerular membrane properties. to determine the morphologic and ultrastructural correlates of this disorder a rat model was developed and 32 postobstructed kidneys were studied by light and electron microscopy at the midpoint of diuresis and compared to 22 controls. the abnormal morphology was: dilated distal tubules and collecting ducts, isolated proximal and distal tubule cells ...	1976	992966
time sequence and site of fluid accumulation in experimental neurogenic pulmonary edema.	the initial phase of pulmonary edema development following intracranial pressure elevation was studied by means of transmission electron microscopy. using perfusion fixation and application of a blood tracer (hrp horseradish peroxidase) the time sequence and site of fluid leakage out of pulmonary vessels was demonstrated: - passage of edema fluid through intercellular clefts of alveolar capillary endothelium - edema accumulation in alveolar interstitial tissue - draining of edema fluid from the ...	1976	996374
electron microscopic cytochemical localization of intravenously injected liposome-encapsulated horseradish peroxidase in rat liver cells.		1976	998408
kinetics of horseradish peroxidase migration through cerebral cortex.	this study provides a semiquantitative description of the migration of the extracellular space marker, horseradish peroxidase (hrp), through the cerebral cortex. following a continuous subarachnoid infusion of hrp, this marker was fixed rapidly within the cortical extracellular space (ecs) by intravascular aldehyde perfusion fixation. microscopic measurements of the maximum depth of penetration of hrp into the cortex perpendicular to the pial surface were taken from coronal whole brain sections ...	1976	1000288
cytochemical localization of glycoconjugate in mitochondria.	complex carbohydrates and cations have been localized by cytochemical methods in mitochondria of mammalian leukocytes, hepatocytes and oyster gill epithelium. glycoconjugate of acidic nature was visualized with the dialyzed iron method in or on the outer membrane and inner boundary membrane and in the outer intermembrane space but not on the membranes of cristae or in the intracristate space, or matrix. sulfated glycoconjugate was demonstrated with the high iron diamine method in a similar distr ...	1976	1002972
arterial hypotension induced by horseradish peroxidase in various rat strains.	the influence of various commercial preparations of horseradish peroxidase upon the arterial pressure has been investigated using direct intra-arterial pressure recordings in nembutal anesthetized rats of different strains. a marked hypotension was noted in wistar and sprague-dawley rats when sufficiently high doses (5-10 mg/100 g body weight) were injected intravenously. this effect was observed with both highly purified (sigma type vi, boehringer reinheitsgrad i) as well as less pure preparati ...	1976	1002975
cerebrovasculature permeability changes following experimental cerebral angiography. a light- and electron-microscopic study.	morphological alterations of the cerebral vasculature as related to the permeability of plasma proteins and angiographic contrast media following unilateral cerebral angiography were studied. both evans blue albumin and horseradish peroxidase were employed as protein tracers for light and electron microscopy investigation respectively. grey matter regions of the cerebral cortex, cerebellum corpus striatum, hippocampus and midbrain showed the most extensive and consistent leakage of these protein ...	1976	1003253
penetration of protein tracers into the epiretinal portion of the optic nerve in the rabbit nerve.	horseradish peroxidase and fluorescein-labelled globulin were injected into the vitreous of adult and 11- to 13-day-old new zealand albino rabbits. both tracers diffused rapidly into the myelinated nerve fibre layer (the "medullary rays"), which extends over a long distance within the retina in these animals. the tracers penetrated also for some distance into the optic nerve beyond the lamina cribosa, where they were incorporated into a large number of cells in the nerve. this finding of a rapid ...	1976	1003255
polysaccharides in lung alveoli.	rat lung alveolar surfaces and contents were studied after using concanavalin a as a bifunctional agent to link exposed sugars to horseradish peroxidase, in accordance with a technique developed by bernhard and avrameas ('71). the graham and karnovsky ('69) diaminobenzidine procedure then was used to provide and electron-dense reaction product so as to define the distribution of complex carbohydrates in alveoli. a layer of very dense raction product was intimately associated with the outer leafl ...	1976	1008038
horseradish peroxidase tracing of the lateral habenular-midbrain raphe nuclei connections in the rat.	connections of the habenular complex to the nuclei of the midline in the midbrain (interpeduncularis, medianus raphe, and dorsalis raphe) have been studied classically by anterograde degeneration in the monkey, the cat, and marsupials. passing fibers from the medial septal nucleus and lateral preoptic area, however, have also been demonstrated which can complicate interpretation of these results. in this paper the habenular projections were studied in the rat by the retrograde axonal transport o ...	1976	1009445
thalamic projections to visually responsive regions of parietal cortex.	thalamic efferents to visually responsive regions of parietal cortex of the cat were investigated by experimental retrograde tracing techniques. two classes of photically evoked potentials recorded from the middle suprasylvian gyrus could be distinguished on the basis of waveform and latency. type 1 responses were recorded from the clare-bishop area at the lateral border of the suprasylvian gyrus and type 2 responses were recorded from association response areas on the crown of the suprasylvian ...	1976	1009447
the development of the isthmo-optic nucleus.	the nucleus of origin of centrifugal fibers to the retina (the so-called isthmo-optic nucleus - ion) has been used as a model for the study of the major features of neural development, from the period of cell proliferation until after the formation of its afferent and efferent connections. 3h-thymidine autoradiography has established that in the chick cells of the ion are generated (i.e., become post-mitotic) between the middle of the 5th and the end of the 7th days of incubation. the first-form ...	1976	1016847
[ultrastructural studies on the permeability of the amnion-epithelium for peroxidase and ferritin. contribution on paraplacental metabolism].	by means tracer substances (horseradish peroxidase, ferritin) the permeability of human amnion and umbilical cord (2. to 6. lunar month) has been investigated ultrastructurally. the structure of intercellular cleft permits a passiv transfer of the water and electrolytes in both ways. the findings indicated, that the passage of proteins with a molecular weight of peroxidase predominantly takes place through the intercellular clefts less by means of pinocytosis. ferritin particles can not penetrat ...	1976	1020513
[origin of afferent axons in the striate cortex of the albino rat: a study with horseradish peroxidase].		1976	1031505
coronary microcirculatory factors and cardiac muscle cell injury.	coronary artery ligation with or without reperfusion was carried out in wistar rats to study the role of coronary microcirculatory factors and membrane permeability alteration of cardiac muscle cell in the evolution of cardiac muscle cell injury by using the fine structural extracellular protein tracer, horseradish peroxidase (hrp). the findings were compared with those obtained in noncoronarogenic myocardial injury models following administration of norepinephrine, a pressor, and isoproterenol, ...	1976	1032011
the relationship between storage and secretion of specific antibody by immune lymphoid cells: ultrastructural localization of anti-peroxidase antibodies in plaque-forming cells of the rabbit popliteal lymph node.	the ultrastructure of antibody-forming cells (afc) has been studied in the lymph node cell population from rabbits locally immunized with horseradish peroxydase (po) incorporated in complete freund's adjuvant, and the kinetics of afc development followed from day 7 to day 18 after one injection of po. identification of the afc was done by local hemolysis assay, using carboxymethyl cellulose solidifying medium and po-coated sheep erythrocytes. afc were thereafter transferred by micromanipulation ...	1976	1033066
organization of thalamocortical auditory system in the cat studied with horseradish peroxidase.		1976	1244977
peroxidase activity of alveolar macrophages.	peroxidase activity was studied in alveolar macrophages and compared to the peroxidase activity in polymorphonuclear leukocytes using cytochemical techniques. a dense reaction product for peroxidase was observed in the primary lysosomes of polymorphonuclear leukocytes, but no significant peroxidase or peroxidative enzymes could be detected in rabbit alveolar macrophages. furthermore, following vigorous phagocytosis of zymosan particles by alveolar macrophages in vitro, no peroxidase could be det ...	1976	1246123
the penetration of fluorescein-conjugated and electrondense tracer proteins into xenopus tadpole optic nerves following perineural injection.	the permeability of xenopus tadpole optic nerves to macromolecules was studied in order to evaluate the usefulness of this system for studying mechanisms of serum-induced cns demyelination in vivo. single injections of either horseradish peroxidase (hrp), ferritin or fluorescein-conjugated human igg were injected around the right optic nerve and tadpoles were then sacrificed between 15 min and 48 h. each of the tracers had penetrated the nerve parenchyma by 30 min. entry of hrp and ferritin occu ...	1976	1247883
[experience with the use of horseradish peroxidase for light and electron microscope studies of interneuronal connections].	by the method based on a retrograde axonal transport of exogenous horseradish peroxidase (hrp), the origins of afferentation of the motor cortex of adult cats, kittens and albino rats were studied. hrp-positive neurons were found by light and electron microscopy in the somatosensory cortex (c1) of the ipsilateral hemisphere and in the portions of the cortex of the contralateral hemisphere which were symmetrical to the site of injection of hrp. the disposition of neurons, marked by hrp, in the vt ...	1976	1252130
simultaneous visualization by light microscopy of two pituitary hormones in a single tissue section using a combination of indirect immunohistochemical methods.	two indirect methods involving enzyme-labeled antibodies were used to demonstrate simultaneously two distinct tissue antigens in the same histologic section without a need for antigen-antibody dissociative procedures. sections of rat pituitary gland were incubated with rabbit anti-rat luteinizing hormone followed by goat anti-rabbit gamma-globulin conjugated to horseradish peroxidase. the same sections were then further incubated with monkey anti-rat growth hormone followed by goat anti-monkey g ...	1976	1254941
intracellular application of horseradish peroxidase and its light and electron microscopical appearance in spinocervical tract cells.		1976	1260464
ultrastructural studies of mouse leucocytes showing surface binding of peroxidase-conjugated lectins.	examination in the electron microscope of mouse spleen cells treated with conjugates of wheatgerm agglutinin and horseradish peroxidase has shown the surface of myeloid cells to be heavily stained. resting lymphocytes were unstained but became positive after stimulation by phytohaemagglutinin or concanavalin a, suggesting a relationship between the presence of this surface component and the functional state of the cell and providing a further point of similarity between myeloid and lymphoblastoi ...	1976	1261094
afferents to the cerebellar lateral nucleus. evidence from retrograde transport of horseradish peroxidase after pressure injections through micropipettes.	hrp was injected by pressure from glass capillary micropipettes unilaterally into the lateral nucleus of rat so as to encompass the entire nucleus, but without spread into the interpositus nuclei. the cells of origin of the afferents to the lateral nucleus were studied after retrograde transport of the hrp. the reticulotegmental nucleus of the pons was labelled bilaterally and is the major source of crossed and uncrossed reticular imputs. the pontine nuclei also provide extensive crossed and unc ...	1976	1262557
glomerular permeability to proteins. effects of hemodynamic factors on the distribution of endogenous immunoglobulin g and exogenous catalase in the rat glomerulus.	the distribution of endogenous immunoglobulin g (igg) and exogenous catalase was delineated in the rat glomerulus under normal and abnormal hemodynamic conditions. igg was identified by an ultrastructural immunoperoxidase technique using antirat igg fab fragments conjugated to horseradish peroxidase; catalase was identified by a cytochemical reaction. when superficial glomeruli in anesthetized munich-wistar rats were rapidly fixed in situ by dripping glutaraldehyde onto the renal surface, igg an ...	1976	1263444
transendothelial vesicular transport of protein following compression injury to the spinal cord.	routes of vascular leakage resulting in trauma-induced edema have not been clarified. to explore the problem we followed the fate of intravascular horseradish peroxidase (hrp) after compression injury to the thoracic cord (cats). at 90 seconds and 15 minutes, hrp was confined to the gray matter, occupying perivascular spaces, unexpanded extracellular channels, and cytoplasmic compartments of injured cells. adjoining segments contained similar but lesser deposits. at 4 hours, tracer occupied the ...	1976	1263445
acute hypersensitivity pneumonitis in the rabbit. i. an animal model with horseradish peroxidase as antigen.	an experimental model of acute hypersensitivity pneumonitis was developed in rabbits using horseradish peroxidase as antigen. the lesion, produced by aerosol challenge with horseradish peroxidase 21 days after a single parenteral immunization with horseradish peroxidase and complete freund adjuvant, was characterized by diffuse infiltration of respiratory bronchioles and of alveolar ducts and walls with approxomately equal numbers of mononuclear cells (lymphocytes and macrophages) and polymorph ...	1976	1267257
immunohistochemical localization of lh-producing cells in the adenohypophysis of the japanese quail, coturnix coturnix japonica.	the cells that produce luteinizing hormone (lh) in the adenohypophysis of the japanese quail were identified immunohistochemically using anti-chicken lh serum and horseradish peroxidase-labeled goat anti-rabbit gamma globulin serum. the lh cells are localized in the caudal lobe of the pars distalis. they are elongate in shape shape and are polarized toward the sinusoids, especially in their active states. alterations in size of lh cells are directly related to changes in circulating lh levels as ...	1976	1268921
nmr studies of hemoproteins: ph dependence of ferric horseradish peroxidase and horse heart myoglobin.		1976	1269749
the structure of lymphatic capillaries in lymph formation.	the lymphatic vascular system consists of endothelial lined vessels which begin as blind-end tubes or saccules that are located within the connective tissue areas. this system serves as a one-way drainage apparatus for the removal of diffusible substances as well as plasma proteins that escape the blood capillaries. if permitted to accumulate, these escaped components would deplete the circulatory system of its plasma colloids and disrupt the balance of forces responsible for the control of flui ...	1976	1269772
projections of the locus coeruleus and adjacent pontine tegmentum in the cat.	the projections of the locus coeruleus and adjacent pontine tegmentum have been studied using anatomical and physiological methods in the cat. axonal trajectories were traced using either the fink-heimer i method following electrolytic lesions, or the autoradiographic method after injection of tritiated proline into the nucleus. results with both methods were similar. axons of locus noeruleus neurons ascended ipsilaterally through the mesencephalon lateral to the medial longitudinal fasiculus, v ...	1976	1270609
convergent thalamic and mesencephalic projections to the anterior medial cortex in the rat.	small microelectrophoretic deposits of horseradish-peroxidase (hrp) were placed at various loci within the gray matter of the rat's anterior medial cortex. a comparison of the patterns of retrograde cell-labeling charted in 26 such cases confirmed earlier findings in fiber-degeneration studies according to which the respective thalamocortical projections of the mediodorsal (md) and anteromedial nucleus (am) overlap each other over a wide region of the anterior medial cortex. this region of thala ...	1976	1270614
the olivocerebellar projection in the cat as studied with the method of retrograde axonal transport of horseradish peroxidase. ii. the projection to the uvula.	following injections of small quantities of horseradish peroxidase (hrp) suspension in the uvula of the cat, the distribution of labeled cells in the inferior olive has been mapped. the findings confirm the conclusion made on the basis of studies of retrograde cell loss in the olive following ablations of the uvula (brodal, '40b) that two small olivary subdivisions, the nucleus beta and the dorsomedial cell column project heavily to the uvula. in addition the hrp-study shows that the uvula recei ...	1976	1270615
scanning electron microscopy of the subarachnoid space in the dog. iv. subarachnoid macrophages.	young dogs of both sexes were used in this study. transmission and scanning electron microscopy were utilized for the examination of the spinal cord and choroid plexus with emphasis on the study of free cells. these procedures were modified so that, in certain cases, the same cells observed in scanning electron microscopy could be analyzed internally by transmission electron microscopy. one half of the animals were injected under anesthesia with horseradish peroxidase for observation of phagocyt ...	1976	1270624
development of an enzyme immunoassay for human placental lactogen using labelled antibodies.	an immunoassay for the determination of human placental lactogen (hpl) has been developed using specific antibodies labelled with horseradish peroxidase. a method is presented in which the enzyme : antibody conjugate is purified with an antigenic solid phase to ensure the production of conjugates with intact immunological activity. serum components interfere with the assay but this problem can only be avoided at the expense of a precise and practical assay for the determination of hpl.	1976	1270811
permeability alteration of sarcolemmal membrane in catecholamine-induced cardiac muscle cell injury. in vivo studies with fine structural diffusion tracer horse radish peroxidase.	cardiac muscle cell injury was produced in male sprague-dawley rats weighing 300 to 450 gm. with catecholamines, norepinephrine, and isoproterenol; sarcolemmal membrane alteration was tested in vivo using the extracellular macromolecular tracer, horseradish peroxidase. norepinephrine was administered in continuous intravenous infusion in a dose of 4 to 6 mug. per 100 gm. of body weight per minute, whereas isoproterenol was given as a single subcutaneous injection in a dose of 8.5 mg. per 100 gm. ...	1976	1271749
endocytosis by vascular smooth muscle cells in vivo and in vitro. roles or vesicles and lysosomes.	overloading of lysosomes of smooth muscle cells with excess substrate may be a key event in the development of hypertensive and atherosclerotic vascular disease. cellular uptake of materials and its relation to lysosomal function were studied by ultrastructural cytochemistry in aortic smooth muscle cells grown in vitro and in the intact animal. injection of horseradish peroxidase (hrp) into hypertensive rats resulted in rapid insudation of the material into the environs of medial smooth muscle c ...	1976	1275059
direct projections from hypothalamus to hippocampus in the rat demonstrated by retrograde transport of horseradish peroxidase.		1976	1276883
an improved technique for the microinjection of horseradish peroxidase.		1976	1276885
changes in the distribution of the dentate gyrus associational system following unilateral or bilateral entorhinal lesions in the adult rat.	the distribution of the dentate gyrus associational system was analyzed in naive adult rats and in those with either unilateral or bilateral lesions of the entorhinal cortex. horseradish peroxidase histochemistry was used to trace the origin and course of this intrinsic fiber system. the fibers originated in the ca3-4 pyramidal cell field, apparently medial to the origin of the schaffer collateral system, and followed a trajectory which was essentially identical to that described for this system ...	1976	1276951
ultrastructure and permeability of lymph node microvasculature in the mouse.	the microvasculature of lymph nodes and peyer's patches consists of arterioles, capillaries and venules. the postcapillary segment comprises high-endothelial venules (he venules) as well as ordinary venules. in order to study the ultrastructure of the microvasculature, particularly with respect to the nature of intercellular junctions, lanthanum and ruthenium red were used as tracers. furthermore, to evaluate the permeability properties of the different segments of the microvasculature, intraven ...	1976	1277282
cooperative binding of concanavalin a to thymocytes at 4 degrees c and micro-redistribution of concanavalin a receptors.	the mode of binding of 125i-labelled concanavalin a and succinyl-concanavalin a to rat thymocytes at 4 degrees c was investigated. simultaneously, the free binding sites of the cell-bound lectin molecules were quantified by horseradish peroxidase binding. concanavalin a showed cooperative binding while succinyl-concanavalin a did not. the number of molecules of concanavalin a bound to the cell surface when it was saturated was twice the number of molecules of succinyl-concanavalin a. we interpre ...	1976	1278183
uptake of horseradish peroxidase (hrp) by the endothelium of the short-term stored rabbit cornea.	in vitro study of the uptake of horseradish peroxidase (hrp) by the corneal endothelium was carried out on rabbit corneae which were stored in a preserving medium for various periods. the stored corneae were incubated in hrp solution with or without adenosine triphosphate (atp). the number of pinocytotic vesicles decreased as the storage time was prolonged. addition of atp to the hrp solution led to an increase in the pinocytotic vesicles in all storage periods. the pinocytosis in the 12-day sto ...	1976	1083694
interdigitating reticulum cells in the popliteal lymph node of the rat. an ultrastructural and cytochemical study.	electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (idc) in the popliteal lymph node of the rat. the morphological features of the idc of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. this is considered to be an argument in favour of the common origin of idc's and macrophages. ultrahistochemical studies with horseradish peroxidase (hrp) reveal no phagocytotic capacity of i ...	1976	1084780
anatomical and physiological evidence for a cerebellar nucleo-cortical projection in the cat.	combined neuroanatomical and electrophysiological experiments were performed to test the hypothesis that axon collaterals of neurons in the cerebellar nuclei project to the cerebellar cortex in cats. the anatomical studies demonstrated that (a) following the injection of tritiated leucine into the deep cerebellar nuclei, labeled fibers could be traced into the granular layer of the cerebellar cortex, and (b) following the injection of horseradish peroxidase into the cerebellar cortex, retrograde ...	1976	11370232
morphological correlates of visual field transformation in the corpus callosum.	horseradish peroxidase (hrp) injections at the boundary of areas 17 and 18 in cat visual cortex resulted in retrograde labelling of neurones located in contralateral areas 17 and 18. the hrp-positive neurones were spread over a region located around the representation of the vertical meridian; they were most numerous in the locus for area centralis. layer iii pyramidal cells and stellate cells in upper layer iv were identified as the principal neurones of origin of the visual callosal pathway.	1976	19604766
substantia nigra projections to the reticular formation, superior colliculus and central gray in the rat, cat and monkey.	after horseradish peroxidase injections in different parts of the lower brain stem retrogradely labeled neurons were observed in the substantia nigra, mainly ipsilateral pars reticulata. these findings demonstrate that the substantia nigra projects not only to the striatum and thalamus but also to brain stem areas which give rise to descending spinal projections. these nigral projections to brain stem structures may play an important role in a variety of behaviors and may be involved in clinical ...	1976	19604767
projections to gyrus sigmoideus from the substantia nigra in the cat, as revealed by the horseradish peroxidase retrograde transport technique.	after injections of horseradish peroxidase in the gyrus sigmoideus of the cat frontal cortex, labelled neurones were found in the substantia nigra and the ventral tegmental area of tsai. neurones distributed bilaterally clearly predominated in the side of the injection in the rostral sections, but differences in number were less at caudal levels. the present findings confirm the existence of a direct mesencephalocortical projection from this ventral tegmental region, and provide a morphological ...	1976	19604816
combined light and electron microscopic tracing of neurons, including axons and synaptic terminals, after intracellular injection of horseradish peroxidase.	by using intracellular deposition of horseradish peroxidase (hrp), a method has been designed for combined light and electron microscopic tracing of single fibers and synaptic terminals, belonging to physiologically characterized neurons. this technique has been successfully tested on the axon collateral systems of cat alpha-motoneurons, but should also be applicable to other types of neurons.	1976	19604850
the absence of horseradish peroxidase uptake by cerebellar afferents to the red nucleus of the cat.	horseradish peroxidase (hrp) administered to the red nucleus (rn) failed to be transported retrogradely in the axons of the cerebellorubral projection system. this negative result was independent of the anaesthesia, fixation method, injection time or survival period. the absence of hrp uptake and transport in our work reflects some uncertainty in the general usefulness of the hrp technique.	1976	19604851
simultaneous visualization of chromatolytic neurons and neurons retrogradely labeled with horseradish peroxidase.	a method is described for visualization of neurons retrogradely labeled with horseradish peroxidase (hrp) in celloidin-embedded brain slabs. the preservation of cellular detail obtained with this technique facilitates (1) accurate delimination of nuclei or laminae in which hrp-positive neurons are found and (2) simultaneous identification of cells of origin of two central fiber systems, by combining the retrograde transport of hrp and the retrotrade degeneration technique. the latter approach ha ...	1976	19604881
spinocervical tract cells in cat and dog, labeled by the retrograde transport of horseradish peroxidase.	the location of cells of origin of the spinocervical tract has been determined in cat and dog by injecting horseradish peroxidase into the lateral cervical nucleus (lcn). retrogradely labeled cells were found predominantly in the ipsilateral nucleus proprius of the dorsal horn along the entire spinal cord. cells of clarke's column and contralateral ventral horn cells were not labeled, in agreement with previous electrophysiological indications that the spinocerebellar tracts do not maintain coll ...	1976	19604882
cells of origin of hypothalamo-spinal projections in the rat.	the retrograde transport of horseradish peroxidase (hrp) was used to determine the distribution of hypothalamic neurons which send axonal projections to the spinal cord. labeled cells were observed in the paraventricular nucleus (pv) and in the lateral hypothalamic area following hrp injections into the lower thoracic and upper lumbar spinal cords of rats. in pv, the hrp-labeled cells were located in the parvocellular portion of the nucleus. hypothalamo-spinal projections are predominantly uncro ...	1976	19604883
retrograde labeling of central nervous pathways with tritiated or evans blue-labeled bovine serum albumin.	in an attempt to develop a technique for the retrograde tracing of neuronal pathways which does not depend on enzyme activity of horseradish peroxidase (hrp), we have examined the retrograde transport of bovine serum albumin (bsa) by neurons of the central nervous system. following injections of either tritiated or evans blue-labeled bsa into the hippocampal formation of the rat, labeled neurons were found in the ipsilateral entorhinal area in a pattern which was highly reminiscent of that obtai ...	1976	19604885
golgi-like staining of neocortical neurons using retrogradely transported horseradish peroxidase.	neocortical pyramidal neurons stained solidly with horseradish peroxidase (hrp) two days after striatal, thalamic or cortical hrp injections are described in the rabbit, rat and marmoset monkey. the dendritic detail seen in these solid hrp-labeled neurons approaches that produced by the golgi method.	1976	19604892
cells of origin of cortical projections to dorsal column nuclei, spinal cord and bulbar medial reticular formation in the rhesus monkey.	cells of origin of cortical fibers to the dorsal column nuclei, spinal cord and bulbar medial reticular formation were identified in 8 rhesus monkeys by means of the horseradish peroxidase (hrp) retrograde transport technique. after dorsal column nuclei injections small pyramidal neurons were labeled in the contralateral hemisphere in layer v of areas 3, 1, 2 and 5 and in layer v of the posterior part of area 4. in contrast, after spinal cord injections many area 4 giant betz cells in the contra ...	1976	19604894
surface membrane carbohydrate alterations of a flagellated protozoan mediated by bacterial endosymbiotes.	crithidia oncopelti, a parasitic trypanosomatid protozoan of insects, normally contains intracellular symbiotic bacteria. as shown earlier, the protozoa can be rid of their endosymbiotes by chloramphenicol, producing a symbiote-free cell line. here surface-membrane carbohydrate ligands of the symbiote-containing and symbiote-free strains were compared by lectin-mediated agglutination, lectin-ultrastructure localization. [3h] lectin-binding, and fluorescent lectin staining. symbiote-free organism ...	1976	1062797
covalent structure of the glycoprotein horseradish peroxidase (ec 1.11.1.7).		1976	1001465
[detection of antinuclear factor with horseradish peroxidase-conjugated antibody].		1976	798712
centrifugal fibers to the eye in a nonavian vertebrate: source revealed by horseradish peroxidase studies.	a source of efferent fibers to the eye of snakes of the genus thamnophis has been identified by the use of the retrograde transport of horseradish peroxidase. cell bodies of the contralateral nucleus of the ventral supraoptic decussation accumulate horseradish peroxidase after intraocular but not intraorbital injections. intraocular injections also result in anterograde transport of horseradish peroxidase to retinofugal axon terminals. intraorbital injections result in accumulation of horseradis ...	1976	63147
rhombo-mesencephalic connections in the fast conducting electrosensory system of the mormyrid fish, gnathonemus petersii. an hrp study.	using retrograde horseradish peroxidase (hrp) axonal transport direct rhombo-mesencephalic connections were revealed in gnathonemus petersii. by hrp injection in the neuropil of the extrolateral mesencephalic ganglion the neurons of the lateral line lobe nucleus were labeled. the pathway is mainly composed of crossed fibers.	1976	19604893
receptive fields, geometry and conduction block of sensory neurones in the central nervous system of the leech.	1. in segmental ganglia of the leech, the cutaneous mechanosensory neurones responding to to touch innervated the skin of their own segment and of part of the anterior and posterior adjacent segments. each touch receptive field could be divided into three non-overlapping areas: a central part innervated by the branches of the cell which ran in the nerve roots of the ganglion containing the cell body, and anterior and posterior parts innervated by its branches which ran in the nerve roots of the ...	1976	1018277
physiological properties and receptive fields of mechanosensory neurones in the head ganglion of the leech: comparison with homologous cells in segmental ganglia.	a study of the head ganglion of the leech was made to compare the properties of specific sensory cells in this ganglion with those of homologous neurones in the segmental ganglia. 1. in the head ganglion, cells were identified that had electrical properties, sensory modalities and adaptation properties similar to those of touch (t), pressure (p) and nociceptive (n) cells in the segmental ganglia. the cell bodies of these neurones were situated in characteristics positions that could be correlate ...	1976	1018276
a horseradish peroxidase method for the identification of the efferents of acetyl cholinesterase-containing neurons.		1976	826585
[problems posed by the interpretation of peroxidase labelling of various neurons in cyprinidae].	the origin of a centrifugal visual pathway in cyprinids could not be demonstrated with the technique involving the labelling of cell bodies by retrograde transport of horseradish peroxidase. the hypothalamic labelling following intraocular injection of hrp is localized in neurosecretory structures which take up the enzyme that has passed into the circulatory system. identical results were obtained following direct intracardiac injection. thus extreme caution must be taken in attempting to interp ...	1976	827368
the shapes of sensory and motor neurones and the distribution of their synapses in ganglia of the leech: a study using intracellular injection of horseradish peroxidase.		1976	12513
effects of apomorphine and haloperidol on somatopetal transport of horseradish peroxidase in the nigro-striatal pathway.	labeling of cell bodies in pars compacta of substantia nigra (snc) following injections of horseradish peroxidase (hrp) in the neostriatum was not affected by repeated systemic injections of apomorphine or haloperidol during the survival time. these drugs decelerate and accelerate, respectively, the firing of snc neurons. provided the used standard method was sensitive enough, it could be concluded that at least in these neurons the somatopetal transport and accumulation of the tracer do not dep ...	1976	19604884
compound x. an intermediate in enzymatic halogenation.	previous studies have shown that chlorite serves as a halogenation substrate for horseradish peroxidase. in its substrate role, chlorite serves both as a halogen donor and as a source of oxidizing equivalents in the chlorination reaction. we now show that a new spectral intermediate, which we have termed compound x, can be detected as the initial product of the reaction of chlorite with horseradish peroxidase. the reaction of chlorite with horseradish peroxidase to form compound x is a relativel ...	1976	10296
permeability of the amphibian pars intermedia to peroxidase injected intravascularly.	to study the penetration of protein from the blood into and through the pars intermedia of anurans, the electron-dense tracer, horseradish peroxidase (hrp), was injected into the vascular system of the frog, rana pipiens. pinocytotic vesicles were seen along plasma membranes of the stellate cells abutting the pericapillary spaces between the pars intermedia and pars nervosa. the hrp was seen within both the pericapillary spaces and these pinocytotic vesicles. in addition, the hrp was very promin ...	1976	1086719
stoichiometry of the reaction between horseradish peroxidase and p-cresol.	over a wide range of ph horseradish peroxidase compound i can be reduced quantitatively via compound ii to the native enzyme by only 1 molar equivalent of p-cresol. since 2 molar equivalents of electrons are required for the single turnover of the enzymatic cycle, p-cresol behaves as a 2-electron reductant. with p-cresol and compound i in a 1:1 ratio compound ii and p-methylphenoxy radicals are obtained in the transient state. compound ii is then reduced to the native enzyme. a possible explanat ...	1976	9412
oxidation of p-cresol by horseradish peroxidase compound i.	rate constants for the reaction between horseradish peroxidase compound i and p-cresol have been determined at several values of ph between 2.98 and 10.81. these rate constants were used to construct a log (rate) versus ph profile from which it is readily seen that the most reactive form of the enzyme is its most basic form within this ph range so that base catalysis is occurring. at the maximum rate a second order rate constant of (5.1 +/- 0.3) x 10(-7) m-1 s-1 at 25 degrees is obtained. the ac ...	1976	9411
[stabilization of polyacrylamide gel immobilized horseradish peroxidase by its covalent coupling to albumin].	pretreatment of peroxidase by its covalent coupling to inert proteins and albumin by means of glutaraldehyde considerably increases the thermostability and specific activity of polyacrylamide gel (paag) immobilized peroxidase. the effects of paag composition on the catalytic properties of the immobilized oligomers: peroxidase-inert proteins-albumin, are studied. the oligomers immobilized in 40% paag (10% n,n'-methylenebisacrylamide) possess the maximal specific activity (4.5 nmol/g). the effects ...	1976	1024581
preparation and properties of horseradish peroxidase bound covalently to polystyrene beads. application in the semi-automatic determination of hydrogen peroxide with homovanillic acid as substrate.		1976	973687
ultrastructural localization of immunoglobulins in skin of patients with dermatitis herpetiformis.	a multistep immunocytochemical method utilizing horseradish peroxidase as an immunologically bound marker was used to detect and localize iga deposits in skin of patients with dermatitis herpetiformis at the ultrastructural level. iga was found in the upper papillary dermis forming irregular aggregates in seemingly haphazard distribution. these aggregates were associated with microfibrillar bundles and with the microfibrillar component of the elastic tissue. iga was also detected on anchoring fi ...	1976	972258
studies on intra-arterial cushions. ii. distribution of horseradish peroxidase in cushions at the origins of intercostal arteries in mice.	the distribution of horseradish peroxidase (hrp) in intraarterial cushions at the origins of intercostal arteries was studied in mice 30 min after intravenous injection of the tracer. hrp is found to be evenly distributed in the extracellular space of the entire arterial wall. basal lamina material is penetrated by the tracer protein. this is not the case with collagenic fibrils, elastic material and intercellular granular deposits which occur regularly in cushions of adult mice but not in those ...	1976	984469
characterization of the chromophores in horseradish peroxidase compounds i and ii using magnetic circular dichroism.		1976	985498
the oxidation of dithiothreitol by peroxidases and oxygen.	horseradish peroxidase (1.11.1.7), lactoperoxidase (1.11.1.7), and the fragment of cytochrome c known as microperoxidase have been shown to catalyze the oxidation of reduced dithiothreitol in an oxygen-consuming reaction. evidence for horseradish peroxidase intermediates compound iii and compound ii has been observed, although ferroperoxidase was not identified during the course of the reaction. the stoichiometry has been extablished as 1 : 1 for oxygen consumed to dithiothreitol oxidized. cyste ...	1976	953035
unit cell dimensions of crystalline horseradish peroxidase.		1976	972399
simultaneous demonstration of horseradish peroxidase and acetylcholinesterase.	simultaneous demonstration of acetylcholinesterase (ache) and horseradish peroxidase (hrp) has been achieved by staining frozen sections with a modified koelle-friedenwald thiocholine method for ache followed directly by the graham-karnovsky procedure for hrp. by using sodium sulfite instead of sodium sulfide in the ache procedure, the final reaction product appears as black ache granules that contrast sharply with the yellowish-brown hrp granules.	1976	19604859
anatomical analysis of ventrolateral thalamic input to primate motor cortex.	1. the origin and topographical organization of input to the arm area of the primate motor cortex from the ventrolateral thalamus were examined using the method of retrograde transport of horseradish peroxidase (hrp). 2. a thin, continuous slab of labeled neurons was found in the ventrolateral thalamus followingmultiple injections of hrp into the arm area of the motor cortex. the slab of labeled neurons was flanked, medially and laterally, by groups of unlabeled neurons. 3. the origin of ventrol ...	1976	62039
[catalytic properties and stability of horseradish peroxidase immobilized in polyacrylamide gel].	effect of polyacrylamide (paa) gel on properties of horseradish peroxidase, immobilized by means of the incorporation into paa gel is studied. catalytic properties of immobilized enzyme are studied. km value and ph-dependency of the enzyme activity are found to be close to those of soluble enzyme, kcat value is 3 times lower at ph 7.0. ph-stability of immobilized peroxidase at 20 degrees c and thermostability of soluble and immobilized peroxidases at ph 7.0 within the temperature range from 20 t ...	1976	10016
increased permeability of cerebral vessels to horseradish peroxidase induced by ischemia in mongolian gerbils.	cerebral ischemia was induced by occlusion of the left common carotic artery in adult mongolian gerbils. the period of occlusion was 3, 6, or 18 h. horseradish peroxidase (hrp) was intravenously injected in animals with clear neurological signs 1 h release of the clip. the hrp was allowed to circulate for 5 min. fixation was carried out by perfusion with aldehydes. tissue, incubated for peroxidatic activity, from the left side of the brain was treated for electron microscopy. during the postisch ...	1976	961382
resonance raman evidence for fe (iv) in compound ii of horseradish peroxidase.		1976	962956
concanavalin a-horseradish peroxidase bridge staining of alpha-1 glycoproteins separated by isoelectric focusing on polyacrylamide gel.	the coomassie blue protein stain and the periodic acid-schiff stain for glycoproteins are compared to a new method of staining glycoproteins resolved electrophoretically. the method utilizes a concanavalin a-horseradish peroxidase sequence to visualize selectively glycoproteins with terminal or internal mannose or terminal n-acetylglucosamine. the method applied to characterization of m and z allele products of alpha-l-antitrypsins separated by isoelectric focusing of polyacrylamide gels slabs h ...	1976	60439
substituent effect on the oxidation of phenols and aromatic amines by horseradish peroxidase compound i.	a stopped-flow kinetic study shows that the reduction rate of horseradish peroxidase compound i by phenols and aromatic amines is greatly dependent upon the substituent effect on the benzene ring. morever it has been possible to relate the reduction rate constants of monosubstituted substrates by a linear free-energy relationship (hammett equation). the correlation of log (rate constants) with sigma values (hammett equation) and the absence of correlation with sigma+ values (okamoto-brown equati ...	1976	954758
mechanisms of electron transfer from sulfite to horseradish peroxidase-hydroperoxide compounds.	using a rapid-scan spectrophotometer equipped with a stopped-flow apparatus, reactions of sulfite with compounds i and ii of two horseradish peroxidase isoenzymes a and c were investigated. the direct two-electron reduction of peroxidase compound i by sulfite occurred at acidic ph but the mechanism gradually changed to the two-step reduction with the intermediate formation of compound ii as the ph increased. the ph at which the one- and two-electron changes occurred at the same speed was 4.5 for ...	1976	8081
synaptic activity of frog retinal photoreceptors. a peroxidase uptake study.	the uptake of horseradish peroxidase (hrp) into membranous structures, detectable by light and electron microscopy, is used here to monitor the synaptic activity of photoreceptors of isolated frog retinas maintained in the dark or under various illumination conditions. the major findings are: (a) neurotransmission from photoreceptor terminals seems to involve the same types of endocytic membrane-retrieval processes that occur at other nerve terminals. presumably, the endocytic processes compensa ...	1976	1084350
peroxidase uptake by photoreceptor terminals of the skate retina.	the photoreceptors of dark-adapted skate retinas bathed in a ringer solution containing horseradish peroxidase (hrp) incorporate the tracer into membrane-bound compartments within the synaptic terminal of the cell; after 1 or 2 h of incubation, approx. 10-38% of the synaptic vesicles were labeled. the receptors appeared to be functioning normally throughout the incubation period, since electrical potentials of normal amplitude could be elicited in response to dimphotic stimuli. however, it was p ...	1976	932103
the kinetics of formation of horseradish peroxidase compound i by reaction with peroxobenzoic acids. ph and peroxo acid substituent effects.	1. the kinetics of formation of horseradish peroxidase compound i were studied by using peroxobenzoic acid and ten substituted peroxobenzoic acids as substrates. kinetic data for the formation of compound i with h2o2 and for the reaction of deuteroferrihaem with h2o2 and peroxobenzoic acids, to form a peroxidatically active intermediate, are included for comparison. 2. the observed second-order rate constants for the formation of compound i with peroxobenzoic acids decrease with increasing ph, i ...	1976	9067
immunocytochemical localization of nerve growth factor (ngf) in the submandibular gland of adult mice by light and electron microscopy.	nerve growth factor (ngf) was localized in the submandibular gland of adult male mice by a direct immunocytochemical method using highly purified antibodies against ngf coupled to horseradish peroxidase. in light microscopic sections the reaction product was entirely confined to the cells of the secretory tubules. the acinar part of the gland was free of reaction product. this finding was confirmed by electron microscopy. within the cells ngf was localized exclusively in the apical secretory gra ...	1976	949726
monosynaptic inputs to caudate neurons identified by intracellular injection of horseradish peroxidase.		1976	1276937
organization of the tectofugal visual pathway in the pigeon: a retrograde transport study.	in birds, superficial laminae of the optic tectum receive a massive retinal input; the tectum in turn projects upon the nucleus rotundus thalami, which then sends its efferents to the ectostriatal core of the telencephalon. to examine the detailed organization of this principal ascending visual pathway, small injections of the marker horseradish peroxidase (hrp) were placed in various sites throughout the ectostriatum (e) or nucleus rotundus (rt) in pigeons. analysis of the resulting patterns of ...	1976	1270632
descending pathways from the brain stem to the spinal cord in some reptiles. i. origin.	in the present study of the origin of the pathways descending from the brain stem to the spinal cord has been investigated in the reptiles testudo hermanni, pseudemys scripta elegans, tupinambis nigropunctatus and python reticulatus. these reptiles, using highly different types of progression, have been selected, because fundamental variations in the organization of the central motor apparatus are to be expected. the origin of the descending pathways has been demonstrated by recording the occurr ...	1976	1270628