Publications

involvement of malate, monophenols, and the superoxide radical in hydrogen peroxide formation by isolated cell walls from horseradish (armoracia lapathifolia gilib.).	peroxidase associated with isolated horseradish cell walls catalyzes the formation of h2o2 in the presence of nadh. the reaction is stimulated by various monophenols, especially of coniferyl alcohol. nadh can be provided by a bound malate dehydrogenase. this system is capable of polymerizing coniferyl alcohol yielding an insoluble dehydrogenation polymer. nadh was found to be oxidized by two different mechanisms, one involving mn(2+), monophenol, and the superoxide radical o2 (·-) in a reaction ...	1977	24420401
photochemical dimerization of ferulic acid by chloroplasts from sorghum.	sorghum (sorghum bicolor) chloroplasts, lamellar fragments, and triton x-100 solubilized preparations catalyze a blue and red light-sensitized oxidation of ferulic acid to its beta-beta-linked dimer and its hydrolysis product, the acid dimer. exogenous superoxide dismutase had no effect, and catalase and 1 to 10 mm kcn inhibited this photooxidative dimerization only in detergent-treated chloroplasts. it is postulated that the final oxidant is h(2)o(2), formed by light-induced photosystem i elect ...	1977	16659796
selective enzymatic hydrolysis of nerve terminal phospholipids by beta-bungarotoxin: biochemical and morphological studies.	beta-bungarotoxin, a presynaptic protein neurotoxin isolated from the venom of the snake bungarus multicinctus, modifies release of neurotransmitter at the neuromuscular junction. the toxin has a potent phospholipase activity toward both natural membranes and phospholipid liposomes. studies of ionic requirements and selective chemical modification demonstrate that beta-bungarotoxin can only modify synaptic transmission under conditions in which the phospholipase is active. morphological studies ...	1977	928454
technical considerations on the use of horseradish peroxidase as a neuronal marker.		1977	917271
retrograde transport of horseradish peroxidase from the nucleus posterior medialis hypothalami to the hippocampus and the medial septum in the pigeon.		1977	896046
hydroxylation of tyrosine by plant peroxidase and mushroom tyrosinase, with and without hydrazine, to retard the oxidation of dopa.	tyrosine was oxidized to dopa by horseradish peroxidase and mushroom tyrosinase. the first step of hydroxylation of tyrosine in the synthesis of melanin was demonstrated by isolation of dopa from the reaction mixture using hydrazine as a selective retardant.	1977	410043
the chemical properties of out-of-equilibrium states of proteins and the role of these states in protein functioning.	the out-of-equilibrium states of several iron-containing proteins (cytochromes c of different origin, haemoglobin, myoglobin, ferredoxin and other non-haem iron proteins, cytochrome c oxidase, horseradish peroxidase) were recorded after fast changes in the active centre (electron reduction of iron, ligand dissociation). strained states result in which the active centre has already been changed and undergone vibrational relaxation but the main part of protein globule is in the 'old', now out-of-e ...	1977	215393
blood-aqueous barrier can be circumvented by lowering intraocular pressure.	rhesus monkeys were injected intravenously with hypertonic urea (9 ml/kg body weight of 30% urea in 10% invert sugar) and the intraocular pressure was measured with an applamatic tonometer. when this pressure reached its minimum (20% of the normal value) horseradish peroxidase (molecular weight 40,000; radius of an equivalent hydrodynamic sphere about 2.5 nm; 0.5 g/kg body weight), was injected intravenously. twenty minutes following peroxidase administration, either aqueous humor was sampled fr ...	1976	813231
structural alterations in the ciliary process and the blood-aqueous barrier of the monkey after systemic urea injections.	the structural alterations induced in the monkey ciliary epithelium by intravascular injection of urea solutions were studied with the light and electron microscopes. arterial injection of urea resulted in destruction of the nonpigmented epithelium and the consequent breakdown of the blood-aqueous barrier. the intravenous injection did not significantly affect the structural integrity of the ciliary epithelium. the intercellular zonulae occludens were not altered and the intercellular pathway fr ...	1976	814818
deoxyribonucleic acid replication in single cells and chromosomes by immunologic techniques.	antibodies to 5-bromodeoxyuridine (brdu) or iododeoxyuridine may be used to identify cells or regions of chromosomes in which de novo deoxyribonucleic acid synthesis has occurred. the antibodies to brdu were produced in rabbits by injection of the antigen, a conjugate between bovine serum albumin and bromouridine (bru), or iodouridine. specific antibodies were produced by affinity chromatography on ah-sepharose 4b to which had been coupled bru. anti-bru cross-reacts with iodeodeoxyuridine. indir ...	1976	815428
midbrain, diencephalic and cortical relationships of the basal nucleus of meynert and associated structures in primates.	the structure and connectivity of the basal nucleus of meynert, the substantia innominata in which it lies, and certain related areas have been examined in new world and old world monkeys, using retrograde and anterograde axonal transport methods. experiments using the retrograde, horseradish peroxidase method confirm the observations of kievet and kuypers ('75) that the basal nucleus and substantia innominata project directly, heavily and with a somewhat crude topography upon the neocortex. exp ...	1976	818134
immunofluorescence and immunoperoxidase observations of anti-lactic dehydrogenase-1 antibody.	for immunoelectron-microscopic demonstration of lactic dehydrogenase (ldh), anti-subunit-b antibody was produced in rabbits by immunization with purified pig ldh-1 (b4) isoenzyme. immunoglobin fragment fab' was obtained from anti-ldh serum immunoglobulin g and conjugated with horseradish peroxidase. subunit-b-containing ldh was present diffusely in the sarcoplasm of the pig heart muscle. there was concentration of ldh in the 1-band. ldh reaction was also present in the mitochondrial intermembran ...	1976	818310
projections from the dorsal lateral geniculate nucleus to prestriate cortex in the squirrel monkey as demonstrated by retrograde transport of horseradish peroxidase.		1976	819105
areal and laminar distribution of some pulvinar cortical efferents in rhesus monkey.	the areal and laminar distribution of the cortical efferents of the medial, lateral and inferior pulvinar nuclei (pm, pl and pi respectively) were determined in rhesus monkey using autoradiography and horseradish peroxidase (hrp). the autoradiographic data indicated that: areas 8a, 45 and 46 on the convexity and 11 and 12 on the orbital surface of the frontal lobe received projections from pm; areas 20, 21 and 22 in temporal lobe received projections from pm primarily with caudal-medial parts of ...	1976	823181
spinal projections from the nucleus locus coeruleus and nucleus subcoeruleus in the cat and monkey as demonstrated by the retrograde transport of horseradish peroxidase.	the rostral pons of the cat and rhesus monkey were examined for the presence of labeled cells following injections of horseradish peroxidase (hrp) into the lumbar spinal cord. labeled cells were found in the ipsilateral dorsolateral pontine tegmentum and in the contralateral ventrolateral pontine reticular formation. in both the cat and monkey, labeled cells were located in the nucleus locus coeruleus, nucleus subcoeruleus, in or near the kölliker-fuse nucleus, and in the ventral part of the lat ...	1976	824030
the effect of alloxan on the permeability of isolated pancreatic islets to horseradish peroxidase.	the effect of alloxan on the permeability of isolated pancreatic islets to horseradish peroxidase was studied by a perifusion system, which allowed simultaneous monitoring of glucose-induced insulin secretion. rat islets were perifused with a 5 mg/ml glucose solution containing horseradish peroxidase for ten or thirty minutes, with or without prior exposure to alloxan (20 mg%) for five minutes. control islets without alloxan treatment showed few necrotic beta cells diffusely infiltrated with exo ...	1976	827103
the mammalian rete testis--a morphological examination.	the fine structure of the rete testis was examined in several primates, domestic animals and rodents. the rete testis consists of a series of interconnected wide channels lined with a simple cuboidal to columnar epithelium, resting on a thick basal lamina. beneath the basal lamina dense bundles of collagen fibrils and a few blood vessels, lymphatics or nerve tissue are found. the epithelial cells are characterized by large, deeply indented nuclei, spherical or short rod-shaped mitochondria, supr ...	1976	827216
experimental retinal branch vein occlusion in the monkey. histopathological and ultrastructural studies.	light and electron microscopical studies of monkey retinae after experimental branch vein occlusion by argon laser reveal that the immediate reaction in the corresponding region is one of capillary engorgement and dilatation followed by oedema (intra- and extracellular), and plasma leakage as shown by intravenous injection of horseradish peroxidase. vascular stasis is characterized by packing of erythrocytes in the capillary lumen, early thrombus formation, and focal haemorrhages. progressive de ...	1976	827828
effect of indomethacin and atropine in experimental asthma in conscious guinea pigs.	pulmonary mechanics were measured in unanesthetized guinea pigs sensitized to horseradish peroxidase (hrp) before and during two aerosolized challenges of this antigen. during the first challenge the pulmonary resistance increased in all animals. prior to second challenge the animals received either atropine (0.2 mg/kg) or indomethacin (10 mg/kg) intraperitoneally. we found that during the second challenge the indomethacin group had an increase in pulmonary resistance slightly greater or similar ...	1976	931927
demonstration of concanavalin a receptors on leptomonas pessoai cell memebrane.	leptomonas pessoai promastigotes cultivated in a synthetic medium were agglutinated with concanavalin a (cona). agglutination was predominantly of the flagellar-flagellar type, and was inhibited with sucrose or alpha-methyl-d-mannoside. cell surface polysaccharides and/or glycoproteins were demonstrated by several cytochemical methods at the fine-structural level. a con a-horseradish peroxidase-diaminobenzidine (dab) technic was used to detect con a receptors in the pellicular membrane of the fl ...	1976	933086
effect of angiotensin-induced hypertension on rat coronary arteries and myocardium.	acute hypertension has been produced in rats by the intravenous infusion of angiotensin amide for 4 hours. both control and hypertensive animals were injected intravenously prior to sacrifice with either horseradish peroxidase (hrp) or colloidal carbon. epicardial arteries and blocks of ventricular myocardium containing intramyocardial arteries and arterioles have been processed for electron microscopy. hrp appears to penetrate the endoethelium of epicardial arteries from control animals within ...	1976	937512
distribution of endogenous albumin in the rat glomerulus: role of hemodynamic factors in glomerular barrier function.	using an ultrastructural immunoperoxidase technique, the distribution of endogenous albumin in the rat glomerulus was delineated under normal and abnormal hemodynamic conditions. superficial glomeruli in anesthetized munich-wistar rats were rapidly fixed in situ by applying glutaraldehyde to the renal surface. fixed tissue slices were treated with anti-rat albumin fab fragments conjugated to horseradish peroxidase (hrp), and were then subjected to the graham-karnovsky ultrastructural peroxidase ...	1976	940256
a new method for the cytochemical demonstration of peroxidase for light, fluorescence and electron microscopy.	a new technique for the cytochemical demonstration of peroxidase is presented. monomeric homovanillic acid is converted by h2o2 and peroxidase into its dimeric form, which is then precipitated as a complex salt of lead and rhodamine 6g or rhodamine b. the reaction product can be visualized by conversion to lead sulphide or viewed directly under the fluorescence microscope, since it emits a red fluorescence when excited with green light. the reaction is rapid and results in good localization at t ...	1976	768373
[immunofluorescence and immunoelectron microscopic study of changes occurring in secondary reaction of popliteal lymph nodes in young and old rats].	a comparative study of the secondary reaction of popliteal lymph nodes in young and adult rats was carried out. in one group of experiments secondary reaction was induced by bovine serum albumin (bsa), in the other one by peroxidase from horseradish. antigen was administered with freund adjuvant. it was established that the index of popliteal lymph nodes in a secondary reaction induced by bsa does not change with the increasing age. the incidence of antibody-producing cells in young and adult ra ...	1976	781524
distribution of dengue-2 antigens by electron immunocytochemistry.	the distribution of dengue-2 antigens was studied in infected monkey kidney cells (llc mk2) using an indirect, horseradish peroxidase-conjugated immunoglobulin technique. this procedure allowed both light and electron microscopic examination of serial-step sections of individual cells cut in a plane perpendicular to the monolayer. both virion and nonvirion antigens were identified on the plasma membrane with this technique. a diffuse cytoplasmic reaction product was also present. the intensity a ...	1976	786880
detection of endogenous immunoreactive prolactin in rat mammary epithelial cells during lactation.	the question of whether endogenous prolactin might be detectable in normally lactating rat mammary glands (lmg) was examined by light microscopic immunohistochemistry. in an initial study, sections of lmg were incubated sequentially with i) rabbit anti-rat prolactin (aprl); ii) anti-rabbit gamma globulin: peroxidase conjugate (argammag-per), prepared from goat anti-rabbit gamma globulin and horseradish peroxidase, and iii) 3,3'-diaminobenzidine mixed with h2o2(dab). use of this three-step proced ...	1976	789055
cell surface saccharides of trypanosoma lewis i. ii. lectin-mediated agglutination and fine-structure cytochemical detection of lectin-binding sites.	bloodstream (bsf) and culture forms (cf) of trypanosoma lewisi were specifically agglutinated with the plant lectins concanavalin a (con a), soybean agglutinin (sba), wheat germ agglutinin (wga), and fucose-binding protein (fbp). lectin-mediated cell agglutination was inhibited, and reversed in the presence of specific lectin-binding saccharides. cells were agglutinated randomly with all lectins suggesting a uniform distribution in the trypanosome cell surface of the lectin-binding saccharide li ...	1976	789385
characterization of macrophage migration inhibitory factor activity produced in vivo by a cell-mediated immune reaction in the guinea pig.	peritoneal fluid from the abdominal cavities of guinea pigs having delayed hypersensitivity to horseradish peroxidase (hrpo) was obtained by a lavage technique before and after i.p. challenge with antigen. macrophage migration inhibitory factor (mif) and macrophage chemotactic factor activities were measured in peritoneal fluids from each animal. chemotactic activity for macrophages was maximal 24 hr after i.p. challenge and was absent thereafter. mif activity was maximal in peritoneal fluid 24 ...	1976	794416
immuno-electron microscopic localization of immunoglobulins in amyloid fibrils of senile plaques.	amyloid fibrils in senile plaques of the brain of patients with senile dementia or alzheimer's disease combined specifically with horseradish peroxidase (hrpo)-labeled rabbit anti-human igg. light and electronmicroscopic immunoperoxidase technique was used to prove this. the fact may mean that immunological factors were involved in the pathogenesis of amyloid fibrils in the senile plaques, and probably also in that of senile dementia or alzheimer's disease.	1976	795258
trans-epidermal elimination of immune deposits in the skin. an immunohistochemical study on immune deposits using horseradish peroxidase as antigen.		1976	798738
[afferent projections to the locus coeruleus nucleus in the cat. study by the horseradish peroxidase technic].	using a retrograde tracer technique with horseradish peroxidase, we have revealed some afferent projections to the locus coeruleus complex from the contralateral pontine tegmentum, raphe nuclei, substantia nigra, nucleus of the solitory tract, dorsal motor nucleus of the vagus and other regions of the ponto-bulbar reticular formation as well as from hypothalamic and preoptic areas.	1976	134760
cellular localization of cyclic amp in periodontal tissues during experimental tooth movement in cats.	using an immune-histochemical method, cyclic amp was localized in cells of periodontal tissues in orthodontically-treated cats. sixteen cats were treated for periods ranging from 1 hour to 4 weeks. fresh, frozen, undecalcified 6 mu sections of the tissues were incubated with rabbit anti-cyclic amp antibodies, followed by sequential incubations with sheep anti-rabbit igg, rabbit anti-peroxidase igg and horseradish peroxidase. in the final step, the peroxidase was demonstrated by the diaminobenzid ...	1976	175896
[further purification and immunohistological localization of human placental lactogen in syncytiotrophoblasts (author's transl)].	in 1936, ehrhadt reported a prolactin-like substance which has been identified as a second protein hormone exists in extracted substance from the placenta. to date various reports have been published, but ther are still unsolved problems concerning the specifity of the substance, and its clinical significance is unclear. the purpose of this project was to purify and investigate the biochemical, immunological properties of hpl, and try to localize hpl secretion immunohistologically. the biologica ...	1976	176070
studies on the chlorinating activity of myeloperoxidase.	two methods were utilized to demonstrate the peroxidation of chloride ion to a free species (hocl or cl2) by myeloperoxidase. the peroxidase caused the volatilization of radioactivity from soultions containing hydrogen peroxide and [36cl]nacl, and catalyzed the formation of hocl when solutions contianing these components were passed through a millipore filter to which the peroxidase was adsorbed. in this flow system, 90 mug of canine myeloperoxidase generated 80 mum hocl in the presence of 200 m ...	1976	176150
use of horseradish peroxidase to study the antagonism of clostridium welchii (cl. perfringens) type d epsilon toxin in mice by the formalinized epsilon prototoxin.		1976	176185
subcellular localization of b apoprotein of plasma lipoproteins in rat liver.	multispecific antigen-binding fragments (fab) from rabbit antisera against rat very low density lipoproteins (vldl) and fab against rat low density lipoproteins that were monospecific for the b apoprotein were conjugated to horseradish peroxidase. conjugates were incubated with 6-mum frozen sections from fresh and perfusion-fixed livers and with tissue chopper sections (40 mum thick) from perfusion-fixed livers. in the light microscope, specific reaction product was present in all hepatocytes of ...	1976	177430
peroxidase-conjugate chromatography isolation of conjugates prepared with glutaraldehyde or periodate using polyacrylamide-agarose gel.	fractionation abilities of polyacrylamide-agarose gel (ultrogel) and dextran gel (sephadex) column chromatography were compared in isolating horseradish peroxidase conjugates, prepared using two different methods. utrogel aca-44 provides an efficient separation of monomer conjugated and nonconjugated immunoglobulins resulting from the two-step glutaraldehyde procedure, sephadex g-200 does not. both types of columns eluted the polymer conjugates resulting from the periodate procedure in the void ...	1976	177698
secretion-related uptake of horseradish peroxidase in neurohypophysial axons.	during secretion of the neurohypophysial hormones, oxytocin and vasopressin, secretory granule membrane is added to the plasma membrane of the axon terminals. it is generally assumed that subsequent internalization of this additional membrane occurs by endocytosis. in order to study this process, we have traced the uptake of intravenously injected horseradish peroxidase by neurohypophysial axons in rats and golden hamsters. peroxidase reaction product within the secretory axons was found mainly ...	1976	181385
direct immunoperoxidase staining for regan isoenzyme of alkaline phosphatase in human tumor tissues.	immunoperoxidase staining for regan isoenzyme of alkaline phosphatase was performed on cryostat sections of five human tumor tisssues. with a direct immunoperoxidase staining for the localization of regan isoenzyme at the light and electron microscope levels, sections previously fixed with 0.05 m phosphate-buffered 4% paraformaldehyde were reacted with rabbit antisera to human placenta alkaline phosphatase conjugated to horseradish peroxidase. comparison of conventional histochemistry and immuno ...	1976	182352
early events in polyoma virus infection: attachment, penetration, and nuclear entry.	the plaque-assay technique was used as a tool to determine the optimal conditions for adsorption of polyoma virions to host cells. using these optimal conditions of adsorption, an electron microscopy study of the early events of infection was performed. by electron microscopy and autoradiography, it was demonstrated that both the viral coat proteins and dna arrive simultaneously in the nucleus as early as 15 min postinfection. when horseradish peroxidase-labeled virions, pseudovirions, and capsi ...	1976	183018
peroxidase and fluorescein isothiocyanate as antibody markers. a quantitative comparison of two peroxidase conjugates prepared with glutaraldehyde or periodate anda fluorescein conjugate.	batches of rabbit anti-human immunoglobulin g antibodies were labeled either with horseradish peroxidase, using the two-step glutaraldehyde method or the periodate method, or with fluorescein isothiocyanate (fitc). the peroxidase conjugates were isolated by chromatography using two different gel types. the five types of conjugates thus obtained were standardized to the same amount of rabbit immunoglobulin g. the antibody activity, as estimated by means of single radial immunodiffusion and passiv ...	1976	184204
beneficial effect of catalase treatment on growth of clostridium perfringens.	several common plating media were tested for their ability to support growth of clostridium perfringens after storage of the plates for 1 to 10 days at 4 and 25 degrees c with and without subsequent addition of catalase. liver-veal (lv) agar and brain heart infusion (bhi) agar quickly become incapable of supporting growth after storage without added catalase, whereas shahidi ferguson perfringens (sfp) agar and brewer anaerobic (ba) agar were less affected. plate counts of c. perfringens on untre ...	1976	185958
prenatal development of central optic pathways in albino rats.	the development of the central optic projections in albino rat fetuses has been studied using light and electron microscopic degeneration techniques and the horseradish peroxidase method for demonstrating axonal projections of neurons. the first optic axons to reach the region of the optic chiasm arrive at day 15. by day 16, a substantial optic chiasm is seen and the optic tract can be traced into the epithalamus, having first passed through the ventral lateral geniculate nucleus and a thin lami ...	1976	54370
the climbing fibers of the cerebellar cortex, their origin and pathways in cat.	the sources and pathways of the climbing fibers to the cerebellar posterior vermis were studied with comibined electrophysiological and anatomical methods in cats. recording from identified cerebellar purkinje cells, monosynaptic climbing fiber (cf) responses have been obtained both for stimulation of the inferior olive (io) and various parts of the brain stem (bs). cf responses were found to of three types, io only, bs only or both io and bs. however the responses to bs stimulation were very fe ...	1976	63384
horseradish peroxidase: an improvement in intracellular staining of single, electrophysiologically characterized neurons.		1976	63386
the anatomical organization of the cerebello-olivary projection in the cat.	the cerebello-olivary pathway in the cat has been examined using orthograde and retrograde neuroanatomical tracing techniques. the orthograde transport of 3h-leucine from injection sites in the deep cerebellar nuclei labeled dentate and interpositus projections to the rostral two-thirds of the contralateral inferior olivary complex. these projections are topographically organized, with the dentate nucleus projecting to the principal olivary nucleus and the posterior and anterior interpositus nuc ...	1976	63472
the blue reaction product in horseradish peroxidase neurohistochemistry: incubation parameters and visibility.	a blue reaction product is formed at sites that contain horseradish peroxidase (hrp) activity when benzidene is used as the chromogen. with neutral red as a counter stain, this method affords excellent visualization of both retrograde and orthograde axonal transport of intracerebrally injected hrp. the visibility of this blue reaction-product is better than the visibility of the brown reaction-product obtained in the commonly used diaminobenzidene procedures. variations in incubation times and r ...	1976	63512
tracing axons and axon collaterals of spinal neurons using intracellular injection of horseradish peroxidase.	intracellular injection and subsequent histochemical localization of horseradish peroxidase have been used to stain the soma, dendrites, axons, and axon collaterals of spinalcervical tract neurons and unidentified dorsal horn neurons in the cat. this technique may be used in combination with the intracellular injection of procion yellow to demonstrate by light microscopy connections between physiologically typed vertebrate neurons.	1976	54936
factors affecting retrograde axonal transport of horseradish peroxidase in the visual system.		1976	55291
light and electron microscopic observations on the anterograde transport of horseradish peroxidase in the optic pathway in the mouse and rat.		1976	55292
electron microscopic immunohistochemical identification of endothelial cells in the rabbit.	antibody to tissue factor apoprotein was adsorbed against gamma-globulin and coupled to horseradish peroxidase; this complex was applied to various rabbit tissues. the distribution of the peroxidase marker then was observed by electron microscopy. we examined fixed or frozen sections, as well as breis of aorta, vena cava, brain, heart, lung, liver, spleen, kidney, bone marrow, mesothelial gut lining, erythrocytes, and platelets. all endothelial cells that had been exposed to the antibody complex ...	1976	55315
retrograde transport of horseradish peroxidase in transected axons. 3. entry into injured axons and subsequent localization in perikaryon.	horseradish peroxidase (hrp) applied to crushed mouse sciatic nerves diffused through the damaged perineurium into the endoneurium. in the injured area, hrp passed into damaged myelinated and unmyelinated axons forming columns of reaction product, which extended for several millimeters proximally to the lesion. ultrastructurally, hrp adhered to the inner surface of the axoplasm and to the surfaces of neurotubules and neurofilaments in such columns. at more proximal levels axons contained hrp in ...	1976	61782
afferents to the rat substantia nigra studied with horseradish peroxidase, with special reference to fibres from the subthalamic nucleus.		1976	61785
a horseradish peroxidase study of the cortico-olivary projection in the cat.		1976	61790
direct projections from the mesodiencephalic midline areas to the pericruciate cortex in the cat: an experimental study with the horseradish peroxidase method.		1976	61792
nigrothalamic projections in the rat as demonstrated by orthograde and retrograde tracing echniques.	the nigrothalamic projection of the rat was investigated by means of horseradish peroxidase (hrp) histochemistry and by the autoradiographic tracing technique. the use of these techniques has permitted an extension of previous descriptions by identifying a neuron population in the substantia nigra, pars reticulata (snr) as the nigrothalamic cells of origin. these calls (20-25 mu) were multipolar and were distributed throughout the snr. there was a tendency however for more labelled neurons to be ...	1976	61793
origins of axons in the cat's acoustic striae determined by injection of horseradish peroxidase into severed tracts.	origins and terminations of fibers of the dorsal and intermediate acoustic striae were studied by surgically severing these tracts and injecting hrp into the incision. this procedure results in filling the severed axons with hrp. filled axons were traced to cell groups of origin and to some terminations of the acoustic striae. hrp-labeled terminals were found in the cochlear nuclei as well as in periolivary cell groups. filling of cells with hrp ranged from being complete, resulting in golgi-lik ...	1976	61976
the origin of the pretecto-olivary tract. a study using the horseradish peroxidase method.		1976	62601
olfactory relationships of the telencephalon and diencephalon in the rabbit. iii. the ipsilateral centrifugal fibers to the olfactory bulbar and retrobulbar formations.	the axoplasmic retrograde transport of horseradish peroxidase (hrp) from axon terminals to their parent cell bodies and histochemical fluorescence microscopy have been used to study the ipsilateral centrifugal fibers to the olfactory bulbs and anterior olfactory nucleus in the rabbit. focal injections of peroxidase were placed unilaterally into the main or accessory olfactory bulb or into the anterior olfactory nucleus. in animals with injected hrp confined within the main bulb, perikarya retrog ...	1976	62770
the use of glutaraldehyde-conjugated horseradish peroxidase-bovine serum albumin in the visualization of concanavalin a binding to tissue sections of human colonic tumor.	a method is reported for the preparation of glutaraldehyde cross-linked horseradish peroxidase conjugates for the visualization of concanavalin a binding to frozen sections. the binding of concanavalin a to tissue sections of human colonic tumor is used to compare staining by horseradish peroxidase conjugates and monomers. without decreasing specificity, the use of progressively larger molecular weight conjugates provides progressively greater intensity of staining of the epithelial components o ...	1976	62892
critical factors involved in the demonstration of horseradish peroxidase retrograde transport.		1976	56209
projection of thalamic neurons to cat primary vestibular cortical fields studied by means of retrograde axonal transport of horseradish peroxidase.	the two vestibular cortical projection areas in the anterior suprasylvian sulcus and post-cruciate dimple regions were defined by evoked potential technique in anaesthetized cats. the thalamic location of neurons with axon terminals in these fields was determined using the method of retrograde axonal transport of horseradish peroxidase. the ascending vestibular pathway appeared to be separated also at the thalamic level, where cells in the ventroposterolateral nucleus were found to project to th ...	1976	56281
ultrastructural differences between the preterminal nerve fibres and their endings in the mechanoreceptors, with special reference to their degeneration and mode of uptake of horseradish peroxidase.		1976	56762
localization of rabbit gamma globulins in the mouse visceral yolk sac placenta.	the yolk sac placenta has been implicated previously in transmission of passive immunity to the fetus. this work uses an immunohistochemical technique devised by sternberger et al. ('69) to study this problem. rabbit serum containing gamma globulins was injected into the uterine lumen of the white swiss mouse during the last third of pregnancy. two or four hours later yolk sacs were removed and fixed in 2% paraformaldehyde or freeze-dried and fixed with paraformaldehyde vapors. finely chopped ti ...	1976	56909
a note on the method of retrograde transport of horseradish peroxidase as a tool in studies of afferent cerebellar connections, particularly those from the inferior olive; with comments on the orthograde transport in purkinje cell axons.	1. injections of horseradish peroxidase (hrp) suspension were made in the cerebellar cortex of cats (most often the paramedian lobule). the staining of the cerebellar cortex and the ensuing labeling of neurons in the inferior olive were studied in experiments with variations of concentration of hrp, amounts of fluid injected, survival time and age of the animals. light microscopical studies were supplemented with electron microscopical observations. the folded cerebellar cortex offers particular ...	1976	57065
movement of horseradish peroxidase in rabbit submandibular glands after ductal injection.	horseradish peroxidase (hrp) has been used as a tracer to study movements of solutions injected retrogradely via the duct of submandibular glands in rabbits. 0.1 ml of solution was injected either manually or by a constant hydrostatic pressure, and the subsequent distribution of hrp in the gland and duct at different times after injection has been examined histochemically at light and electron microscopical levels. shortly after the injections, strong interstitial staining for peroxidase resulte ...	1976	57956
the development of the isthmo-optic tract in the chick, with special reference to the occurrence and correction of developmental errors in the location and connections of isthmo-optic neurons.	the development of the centrifugal projection to the chick retina in the isthmo-optic tract (iot) has been studied by the retrograde transport of the enzyme marker horseradish peroxidase (hrp) injected into the eye at various times during the incubation period. some neurons in the isthmo-optic nucleus (ion--the cells of origin of the iot) can first be labeled from the eye following injections on the tenth day of incubation; after injections on the twelfth day or later, about 95% of the neurons c ...	1976	58875
a guide to the synaptic analysis of the neuropil.	a morphological analysis of the organization of the gray matter in the central nervous system depends on the discovery of consistent repetitive patterns. without these, the gray matter remains a chaotic jungle. an hypothesis derived from the study of a few simple regions has been developed to serve as a guide in finding these patterns. it states that all nerve fibers and terminals arising from a particular group of nerve cells, or, more precisely, a particular nerve cell type, display similar ax ...	1976	59655
anti-streptococcal antibodies reacting with brain tissue. ii. utrastructural studies.	the reaction of rabbit anti-streptococcus pyogenes type 24 with mouse brain has been studied at the ultrastructural level using horseradish peroxidase labelled antiglobulin. it is shown that these antibodies, and by inference those found in sydenham's chorea, react with the fibrous astrocyte. the cross-reacting antigen is the glial filament or a closely associated cytoplasmic constituent.	1976	60120
differences between alpha and gamma motoneurons labeled with horseradish peroxidase by retrograde transport.		1976	60166
immune complex receptors on cell surface. i. ultrastructural demonstration of macrophages.	a method is described for ultrastructural localization of immune complex receptors on the surface of viable peritoneal exudate cells. the technique entails incubation with a soluble complex of horseradish peroxidase (hrp) and specific antibody to hrp at 4 degrees c followed by exposure to diaminobenzidine and processing for electron microscopy. the bound immune complexes were evident as focal deposits of hrp reaction product, adhering closely to the external surface of macrophages with an uninte ...	1976	60441
the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. iii. the projection to the vermal visual area.	horseradish peroxidase (hrp) was injected separately in one of the cerebellar lobules vi, viia, viib, viiia or viiib (together corresponding to the vermal visual area) in 17 cats. after 1-3 days the distribution of labeled cells in the inferior olive was mapped. in spite of some overlapping it is clear that the various lobules of the vermal visual area receive fibers from separate parts of a horseshoeshaped region in the caudal half of the contralateral medial accessory olive (fig. 5c). the proj ...	1976	61211
comparative immunoenzymatic localization of prolactin and growth hormone in human and rat pituitaries.	twelve human and twelve rat pituitaries were stained by an immunohistochemical method using a rabbit anti-ovine prolactin serum, a rabbit anti-human growth hormone serum and a sheep anti-rabbit immunoglobulin serum conjugated with horseradish peroxidase. on the same pituitary section, growth hormone cells were stained brown by using 3-3'-diaminobenzidine as peroxidase substrate, and prolactin cells were stained purplish blue by using 4-chloro-1-naphtol. growth hormone cells outnumbered prolactin ...	1976	61240
distribution of immune deposits in the skin of reversed passive arthus reaction using horseradish peroxidase as antigen.		1976	45717
the topographical distribution of s-100 and gfa proteins in the adult rat brain: an immunohistochemical study using horseradish peroxidase-labelled antibodies.	the cytological and topographical distribution of s-100 and glial fibrillary acidic (gfa) proteins in the adult rat brain has been compared using the horseradish peroxidase-labelled antibody technique. both proteins are present in astrocytes and structures composed of astrocytic processes, namely the glial limitans and the perivascular membranes, but the cytological localization varies between the two proteins. s-100 is found in the nucleus and cytoplasm whereas gfa protein is confined to the cy ...	1976	1107363
descending inputs to caudal cochlear nucleus in cats: a horseradish peroxidase (hrp) study.	after hrp injections into the octopus cell area of the cat cochlear nucleus, only periolivary neurons of the superior olivary complex (soc) reacted. elongate neurons in the lateral periolivary nuclei (ipsilateral to the injection) and multipolar neurons in ventromedial periolivary regions (contralateral to the injection) contained granules. no neurons in the main soc nuclei or higher auditory nuclei reacted, despite a wide range of hrp concentrations. thus, neurons from the soc to the octopus ce ...	1976	941860
correlations between immunoglobulin- and antibody-synthesizing cells during primary and secondary immune responses of rats immunized with peroxidase.	the development of cells synthesizing immunoglobulins without detectable antibody activity and of antibody-synthesizing cells was studied during primary and secondary immune responses of rats immunized with horseradish peroxidase. after primary immunization with peroxidase emulsified in freund's complete or incomplete adjuvant, the first antibody-producing cells appeared 4 days after injection. they were preceded by cells synthesizing igg and igm without antibody function, appearing 3 days after ...	1976	944685
immunoelectron microscopic and immunochemical demonstrations of serum proteins in the alveolar lining material of the rat lung.	by means of antibodies labeled with horseradish peroxidase, autologous albumin and igg were visualized in electron microscopy on the alveolar epithelial surface of the rat lung, as a continuous cell coat, as free granular deposits, or associated with tubular myelin figures. these 2 proteins were detected by immunochemistry in alveolar washing. after ultracentrifugation of alveolar washing, they were still found in the phospholipid-rich precipitate fraction by immunization of rabbits and by immun ...	1976	946160
blood-aqueous barrier in newborn and young rabbits. an electron microscopic study.	distribution of intravenously injected horseradish peroxidase (hrp) in the ciliary body of newborn and young (2 weeks old) rabbits was studied with the electron microscope. the reaction product was localized in all animals in the lumen of the blood vessels, in the perivascular area, in the surrounding connective tissue stroma and also in the epithelial cell layers. the peroxidase penetrated through the intercellular junctions of the pigmented cells to the intercellular space between the apical m ...	1976	946358
the role of suprachiasmatic nuclei of the hypothalamus in the production of circadian rhythm.	(1) action potentials were recorded from single neurons in suprachiasmatic nuclei of hypothalamus in urethane anesthetized male rat. the rate of firing ranged from less than 1 to over 10/sec but was generally 4-8/sec, and it varied from one cell to another in the same animal. (2) repetitive stimulation of optic nerve or light acting on the eye augmented the activity of approximately half of the the suprachiasmatic neurons examined (67 out of 159 cells) while 37 neurons (23%) showed clear inhibit ...	1976	947493
competitive enzyme-liked immunoassay with use of soluble enzyme/antibody immune complexes for labeling. i. measurement of human choriogonadotropin.	we described the principle of a new enzyme-immunoassay, competitive enzyme-liked immunoassay (celia), for quantitative measurement of soluble antigens and haptens. in the assay, binding of antibody to antigen-immunosorbent is competitively inhibited by the free antigen to be measured. the amount of first antibody bound to the immunosorbent is measured by an enzymatic technique in which a heterologous bridging antibody and a soluble antibody/enzyme immune complex are applied in sequence. the solu ...	1976	949847
cortical cells projecting to the dorsal column nuclei of cats. an anatomical study with the horseradish peroxidase technique.	the retrograde transport of horseradish peroxidase (hrp) has been used to label cortical neurons which give origin to descending projections to the dorsal column nuclei (dcn) in kittens and adult cats. hrp was injected unilaterally or bilaterally into the dorsal medulla at, or rostral to, the level of the obex, and, in most cases, retrogradely transported hrp was visualized in cortical neurons by incubation of serial 40 mum coronal or sagittal frozen sections. in the remaining cases, 1 mm corona ...	1976	950388
vascular leakage in the dorsal root ganglia of the rat, studied with horseradish peroxidase.	horseradish peroxidase (hrp) was found to pass from ganglionic blood vessels into the extracellular space of dorsal root and vth nerve ganglia within 2 min of intravenous injection in the rat. by 5 min, the tracer had penetrated into perineuronal and periaxonal spaces. a brisk macrophage response occured, and these cells rapidly engulfed the tracer so that it has almost completely disappeared from the extracellular space 2-3 hr. fenestrated blood vessels, with and without diaphragms were seen wi ...	1976	950578
[the influence of plasmocellular tumours on the humoral immune response in mice (author's transl)].	syngeneic mice of the c3h/he strain have been inoculated with cells of the plasmocellular tumours x5563 and x5647, producing myeloma proteins of the igg and iga class respectively, and were immunized with (1) sheep red blood cells, (2) bovine serum albumin and (3) horseradish peroxydase. the immune responses of the various groups of mice were followed. the antibody activities towards all three antigens were decreased in mice carrying the igg producing tumour x5563. this effect was correlated wit ...	1976	952495
[assay of the activity of horseradish peroxidase antibodies using ammonium sulfate technique (author's transl)].	the antigen binding capacity (abc) of mouse and rabbit antisera to horseradish peroxidase (hrp) was measured by the farr technique, originally introduced for bovine serum albumin. results obtained by this method are independent of other antibody interactions (precipitation, formation of immune complexes, effect on enzyme activity). commercially available hrp has variable solubility in ammonium sulfate solutions; almost 40% was precipitated at 50% saturation. accordingly, the fraction of hrp solu ...	1976	952496
a study of the permeability barrier in epidermis and oral epithelium using horseradish peroxidase as a tracer in vitro.	small biopsies of skin, and of keratinized and non-keratinized oral mucosa were incubated with horseradish peroxidase, fixed and reacted to demonstrate peroxidase activity at the light and electron microscope level. in all tissues peroxidase extended through the intercellular spaces of the epithelium up to, but not into, the stratum corneum of keratinized, or the superficial layer of non-keratinized, tissue. this distribution corresponds to that seen in studies using the tracer in vivo where the ...	1976	952748
cat retinal ganglion cells projecting to the superior colliculus as shown by the horseradish peroxidase method.	1. cat retinal ganglion cells projecting to the superior colliculus (sc) were detected in flat whole-mounts of nissl-stained retinas by means of unilateral intracollicular injections of horseradish peroxidase (hrp). 2. with the purpose of drawing a comparison between hrp-labeled ganglion cells and the remaining population of unlabeled ganglion cells, the latter were classified according to their perikaryal characteristics into three groups: large, medium and small ganglion cells. measurements of ...	1976	954886
immunochemical studies of horseradish peroxidase. factors affecting the inhibition of enzyme activity by specific antibody.		1976	955671
horseradish peroxidase localization of masticatory muscle motoneurons in cat.	horseradish peroxidase has been injected into individual masticatory muscles in young and adult cats in order to determine the topography of the corresponding groups of motoneurons in the motor nucleus of the vth nerve. the results obtained show a clear dorsoventral somatotopic distribution; the superior muscles have their motoneurons located dorsally in the nucleus and the inferior muscles ventrally; the two main jaw closers, temporalis and masseter, are represented in the dorsal and central pa ...	1976	957267
ultrastructural localization of concanavalin a in the perfused rat heart.	staining of the surface coat of heart capillaries and aorta was achieved by perfusing concanavalin a and phytohemagglutinin coupled to horseradish peroxidase through the rat heart. the binding of these lectins provides evidence for the presence of glycoproteins containing mannose, glucose, and n-acetylgalactosamine residues in the endothelial surface coat of rat aorta and heart capillaries. the binding of concanavalin a to capillary endothelium was further followed with radioautography of 125i-l ...	1976	957601
an electron microscopic and cytochemical detection of concanavalin a receptors on the cell membrane of leishmania braziliensis guyanensis.	promastigotes of leishmania braziliensis quyanensis cultivated in the nnn medium agglutinate with concanavalin a (con a). the protozoon was agglutinated at different concentrations of con a. maximal agglutination was obtained with 150 mug/ml. three types of agglutination were observed: flagellar-flagellar, flagellar-body and body-body. cell surface polysaccharides, glycoproteins or glycolipids were demonstrated using the periodic acid-thiosemicarbazide-silver proteinate technic. a con a-horserad ...	1976	961000
reversible blood-brain barrier dysfunction to peroxidase after a small stab wound in the rat cerebral cortex.	small stab wounds were made in the rat frontal lobe. the animals were injected with horseradish peroxidase intravenously at different times after the injury in order to study the extravasation of this tracer. there was a leakage of peroxidase into the brain during the first 3 days after the injury. the route of passage from the vessel lumen into the brain was through disrupted blood vessels in the injured region. endothelial pinocytosis and formation of thin, trans-endothelial channel-like struc ...	1976	961383
cytological observations of the ovarian epithelium in mammals during the reproductive cycle.	we have studied the ovarian epithelijm at various stages of the reproductive cycle in a number of mammalian species utilizing light microscopy, scanning microscopy, the freeze-fracture technique, transmission microscopy and by employing specialized tracers that use lanthanum and horseradish peroxidase. we found that the epithelial cells are joined by incomplete tight junctions, gap junctions, and desmosomes. the cytoplasmic matrix contains a large irregularly shaped nucleus, few microtubules, mi ...	1976	966286
crush injury to peripheral nerve. an electron microscope study employing horseradish peroxidase.	electron microscope observations were made of rat peroneal nerve after crushing using intravenously injected horseradish peroxidase (hrp) as a tracer protein to indicate changes in vascular permeability. at 1/2 h and 2 d after the crush there was gross leakage of hrp from damaged capillaries at the site of injury but none from vessels above or below this. ultrastructurally vessels at the site of crush showed broken and separated endothelial cells. proximally and distally there was little abnorma ...	1976	970115
cells of origin of entorhinal cortical afferents to the hippocampus and fascia dentata of the rat.	the pathway from the entorhinal cortical region to the hippocampal formation has previously been shown to be comprised of two sub-systems, one of which projects predominantly to the ipsilateral fascia dentata and regio inferior of the hippocampus proper, and a second which projects bilaterally to regio superior. the goal of the present investigation was to determine if these two pathways might originate from different cell populations within the entorhinal area. the cells of origin of these ento ...	1976	972204
uptake and transport of exogenous proteins by respiratory epithelium.	the tracer proteins, horseradish peroxidase and ferritin, placed in the trachea of guinea pigs were taken up by epithelial cells and transported to the extracellular space. the interval between the introduction of the tracer proteins into the lumen of the trachea and the morphologic demonstration of the porteins in the extracellular space or within the basal portion of the cells was between 30 and 60 minutes. the proteins were transported in vesicles and no penetration of the epithelial intercel ...	1976	979161
reinnervation of dentate gyrus by homologous afferents following entorhinal cortical lesions in adult rats.	granule cells of the rat dentate gyrus which are denervated by unilateral destruction of the entorhinal cortex are reinnervated in part by proliferation of surviving pathways from the contralateral entorhinal cortex. the cells of origin of these lesion-induced projections were identified by retrograde labeling with horseradish peroxidase and were the same cell type which normally project to the ipsilateral dentate gyrus	1976	982024
ige-induced respiratory and circulatory changes during systemic anaphylaxis in the rabbit.	we studied systemic anaphylaxis induced by the administration of 200 mug of horseradish peroxidase into 11 anesthetized rabbits known to be producing anti-horseradish peroxidase antibodies only of the ige class. ventilatory changes included a transient, abrupt decrease in breathing frequency followed by increased minute ventilation; lung mechanical changes included decreased dynamic lung compliance and increased total pulmonary resistance; cardiovascular changes included pulmonary hypertension, ...	1976	984586
uptake of microperoxidase by segmenting rat ova in vitro.	uptake and incidence of microperoxidase (sigma) in the rat ova during cleavage at the 1-, 2-, 8-cell and blastocyst stages were studied after 30 min incubation with the enzyme (molecular weight of 1,900 and size of molecule of 2 nm). evidence was furnished of the presence of a small amount of reaction product of microperoxidase in the zona pellucida and its local concentration in some parts of the perivitelline space. the larger part of surface of the ovum, however, was free of microperoxidase. ...	1976	986379
quantitative ultrastructural autoradiographic studies of iodinated plasma membranes of lymphocytes during segregation and internalization of surface immunoglobulins.	rat spleen lymphocytes were iodinated (125 i) with lactoperoxidase. quantitative autoradiographic studies on cells fixed immediately after iodination showed 19-24% of intracytoplasmic grains at 3hd and over from the plasma membrane. normalization of grain density distribution and comparison of resulting curves with the universal curve of grain scatter of 125 i showed that a significant percentage of intracytoplasmic grains (36%) originates from intracytoplasmic labeled sources rather than from s ...	1976	986396