Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| an activated by cobalt alkaline aminopeptidase from bacillus mycoides. | an intracellular arginine--specific aminopeptidase synthesized by bacillus mycoides was purified and characterized. the purification procedure for studied aminopeptidase consisted of ammonium sulphate precipitation and three chromatographic steps: anion exchange chromatography and gel permeation chromatography. a molecular weight of -50 kda was estimated for the aminopeptidase by gel permeation chromatography and sds-page. the optimal activity of the enzyme on arginyl-beta-naphthylamide as a sub ... | 2011 | 22808738 |
| early murine immune responses from endotracheal exposures to biotechnology-related bacillus strains. | an immunology-based in vivo screening regime was used to assess the potential pathogenicity of biotechnology-related microbes. strains of bacillus cereus (bc), bacillus subtilis (bs), bacillus thuringiensis (bt), and bt commercial products (cps) were tested. balb/c mice were endotracheally instilled with purified spores, diluted cp, or vegetative cells (vc) (live or dead). exposed mice were evaluated for changes in behavioral and physical symptoms, bacterial clearance, pulmonary granulocytes, an ... | 2011 | 23087536 |
| Exploration of in vitro time point quantitative evaluation of newly synthesized benzimidazole and benzothiazole derivatives as potential antibacterial agents. | Present communication deals with the in vitro time point quantitative antibacterial evaluation of newly synthesized 1,2-disubstituted benzimidazoles (3a-p) and 2-substituted benzothiazoles (5a-h) against Gram-positive bacteria Staphylococcus aureus, Bacillus cereus, and Gram-negative bacteria Vibrio cholerae, Shigella dysenteriae and Escherichia coli. These compounds were synthesized under mild reaction conditions using Al(2)O(3)-Fe(2)O(3) nanocrystals as heterogeneous catalyst. Bio-evaluation s ... | 2011 | 22047695 |
| biosynthesis of cyclodextrin glucosyltransferase by the free and immobilized cells of bacillus cereus nrc7 in batch and continuous cultures. | the objective of this study was to enhance the production of cyclodextrin glucanotransferase (cgtase) produced by a local isolate bacillus cereus nrc7. | 2011 | 21883731 |
| Demonstration of Bacillus cereus in orthopaedic-implant-related infection with use of a multi-primer polymerase chain reaction-mass spectrometric assay: report of two cases. | 2011 | 21915528 | |
| Antibacterial activity of different degree of hydrolysis of palm kernel expeller peptides against spore-forming and non-spore-forming bacteria. | The goal of this study was to determine inhibitory effect of palm kernel expeller (PKE) peptides of different degree of hydrolysis (DH %) against spore-forming bacteria Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus stearothermophillus, Bacillus subtilis, Bacillus thuringiensis, Clostridium perfringens; and non-spore-forming bacteria Escherichia coli, Lisinibacillus sphaericus, Listeria monocytogenes, Pseudomonas a ... | 2011 | 21848644 |
| Bacterial inactivation using silver-coated magnetic nanoparticles as functional antimicrobial agents. | The ability for silver nanoparticles to function as an antibacterial agent while being separable from the target fluids is important for bacterial inactivation in biological fluids. This report describes the analysis of the antimicrobial activities of silver-coated magnetic nanoparticles synthesized by wet chemical methods. The bacterial inactivation of several types of bacteria was analyzed, including Gram-positive bacteria ( Staphylococcus aureus and Bacillus cereus ) and Gram-negative bacte ... | 2011 | 21999710 |
| Isolation and characterization of aerobic culturable arsenic-resistant bacteria from surfacewater and groundwater of Rautahat District, Nepal. | Arsenic (As) contamination of groundwater is a serious Environmental Health Management issue of drinking water sources especially in Terai region of Nepal. Many studies have reported that due to natural abundance of arsenic in the environment, various bacteria have developed different resistance mechanisms for arsenic compound. In this study, the culturable arsenic-resistant bacteria indigenous to surfacewater as well as groundwater from Rautahat District of Nepal were randomly isolated by stand ... | 2011 | 21868146 |
| de-mercurization of wastewater by bacillus cereus (jubt1): growth kinetics, biofilm reactor study and field emission scanning electron microscopic analysis. | removal of mercuric ions by a mercury resistant bacteria, called bacillus cereus (jubt1), isolated from the sludge of a local chlor-alkali industry, has been investigated. growth kinetics of the bacteria have been determined. a multiplicative, non-competitive relationship between sucrose and mercury ions has been observed with respect to bacterial growth. a combination of biofilm reactor, using attached growth of bacillus cereus (jubt1) on rice husk packing, and an activated carbon filter has be ... | 2011 | 21872987 |
| Quality Parameters and Antioxidant and Antibacterial Properties of Some Mexican Honeys. | A total of 14 Mexican honeys were screened for quality parameters including color, moisture, proline, and acidity. Antioxidant properties of complete honey and its methanolic extracts were evaluated by the DPPH, ABTS, and FRAP assays. In addition, the antimicrobial activity of complete honeys against Bacillus cereus ATCC 10876, Listeria monocytogenes Scott A, Salmonella Typhimurium ATCC 14028, and Sthapylococcus aureus ATCC 6538 was determined. Most of honeys analyzed showed values within qual ... | 2011 | 22133067 |
| a multivalent probe for ai-2 quorum-sensing receptors. | multivalency is a common principle in the recognition of cellular receptors, and multivalent agonists and antagonists have played a major role in understanding mammalian cell receptor biology. the study of bacterial cell receptors using similar approaches, however, has lagged behind. herein we describe our efforts toward the development of a dendrimer-based multivalent probe for studying ai-2 quorum-sensing receptors. from these studies, we have discovered a chemical probe specific for lsr-type ... | 2011 | 21913711 |
| metal complexes of diisopropylthiourea: synthesis, characterization and antibacterial studies. | co(ii), cu(ii), zn(ii) and fe(iii) complexes of diisopropylthiourea have been synthesized and characterized by elemental analyses, molar conductivity, magnetic susceptibility, ftir and electronic spectroscopy. the compounds are non-electrolytes in solution and spectroscopic data of the complexes are consistent with 4-coordinate geometry for the metal(ii) complexes and six coordinate octahedral for fe(iii) complex. the complexes were screened for their antibacterial activities against six bacteri ... | 2011 | 22072941 |
| interspecies interaction of signal peptide papr secreted by bacillus cereus and its effect on production of antimicrobial peptide. | this study was carried out to investigate the interspecies interaction of papr peptide secreted by bacillus cereus on production of bsap-254, an antimicrobial peptide produced by bacillus subtilis sc-8 isolated from the korean fermented soybean paste and exhibited narrow antagonistic activity against the b. cereus group, but not against other foodborne pathogens. papr is a signal peptide that activates plcr, which is a pleiotropic regulator controlling the expression of various virulence factors ... | 2011 | 22101448 |
| ywdl in bacillus cereus: its role in germination and exosporium structure. | in members of the bacillus cereus group the outermost layer of the spore is the exosporium, which interacts with hosts and the environment. efforts have been made to identify proteins of the exosporium but only a few have so far been characterised and their role in determining spore architecture and spore function is still poorly understood. we have characterised the exosporium protein, ywdl. δywdl spores have a more fragile exosporium, subject to damage on repeated freeze-thawing, although ther ... | 2011 | 21887322 |
| role of toll-like receptor (tlr) 2 in experimental bacillus cereus endophthalmitis. | bacillus cereus causes a uniquely rapid and blinding intraocular infection, endophthalmitis. b. cereus replicates in the eye, synthesizes numerous toxins, and incites explosive intraocular inflammation. the mechanisms involved in the rapid and explosive intraocular immune response have not been addressed. because toll-like receptors (tlrs) are integral to the initial recognition of organisms during infection, we hypothesized that the uniquely explosive immune response observed during b. cereus e ... | 2011 | 22163046 |
| Transcriptional responses of Bacillus cereus towards challenges with the polysaccharide chitosan. | The antibacterial activity of the polysaccharide chitosan towards different bacterial species has been extensively documented. The response mechanisms of bacteria exposed to this biopolymer and the exact molecular mechanism of action, however, have hardly been investigated. This paper reports the transcriptome profiling using DNA microarrays of the type-strain of Bacillus cereus (ATCC 14579) exposed to subinhibitory concentrations of two water-soluble chitosan preparations with defined chemical ... | 2011 | 21931677 |
| From transcriptional landscapes to the identification of biomarkers for robustness. | The ability of microorganisms to adapt to changing environments and gain cell robustness, challenges the prediction of their history-dependent behaviour. Using our model organism Bacillus cereus, a notorious Gram-positive food spoilage and pathogenic spore-forming bacterium, a strategy will be described that allows for identification of biomarkers for robustness. First an overview will be presented of its two-component systems that generally include a transmembrane sensor histidine kinase and it ... | 2011 | 21995521 |
| evaluation in broilers of the probiotic properties of pichia pastoris and a recombinant p. pastoris containing the clostridium perfringens alpha toxin gene. | the probiotic properties of pichia pastoris and of a recombinant p. pastoris containing the clostridium perfringens alpha toxin gene were evaluated in broilers. one-day-old chicks randomly divided in four groups were fed with commercial feed devoid of antibacterials. the control group (1) received plain food, while the other groups were supplemented with either p. pastoris (2), the recombinant p. pastoris (3) or bacillus cereus var. toyoi (4). at day 49, live weights, feed efficiency and serocon ... | 2011 | 22176763 |
| bacillus manliponensis sp. nov., a new member of the bacillus cereus group isolated from foreshore tidal flat sediment. | a gram-positive, endospore-forming, new bacillus species, strain bl4-6(t), was isolated from tidal flat sediment of the yellow sea. strain bl4-6(t) is a straight rod, with motility by peritrichate flagella. the cell wall contains meso-diaminopimelic acid, and the major respiratory quinone is menaquinone-7. the major fatty acids are iso-c(15:0) and summed feature 3 (containing c(16:1) ω7c/iso-c(15:0) 2oh, and/or iso-c(15:0) 2oh/c(16:1) ω7c). cells are catalase-positive and oxidase-negative. the g ... | 2011 | 22203569 |
| diversity, mobility, and structural and functional evolution of group ii introns carrying an unusual 3' extension. | abstract: background: group ii introns are widespread genetic elements endowed with a dual functionality. they are catalytic rnas (ribozymes) that are able of self-splicing and they are also mobile retroelements that can invade genomic dna. the group ii intron rna secondary structure is typically made up of six domains. however, a number of unusual group ii introns carrying a unique extension of 53-56 nucleotides at the 3' end have been identified previously in bacteria of the bacillus cereus ... | 2011 | 22204608 |
| preparation and antimicrobial activity of poly (vinyl chloride)/gelatin/montmorillonite biocomposite films. | the aim of this study was using a novel antimicrobial thermoplastic plasticizer based on aliphatic anhydride derivative dodecenyl succinic anhydride (dsa) for blending poly (vinyl chloride), pvc, with gelatin in presence of montmorillonite (mmt) using brabender via polymer melting technique. this anhydride-based plasticizer blended the membrane ingredients homogenously under melting process. the used plasticizer exhibited high performance antimicrobial potency for some biomedical and industrial ... | 2011 | 21909641 |
| β-lysine discrimination by lysyl-trna synthetase. | elongation factor p is modified with (r)-β-lysine by the lysyl-trna synthetase (lysrs) paralog poxa. poxa specificity is orthogonal to lysrs, despite their high similarity. to investigate α- and β-lysine recognition by lysrs and poxa, amino acid replacements were made in the lysrs active site guided by the poxa structure. a233s lysrs behaved as wild type with α-lysine, while the g469a and a233s/g469a variants decreased stable α-lysyl-adenylate formation. a233s lysrs recognized β-lysine better th ... | 2011 | 21925499 |
| vigorous cleaning and adequate ventilation are necessary to control an outbreak in a neonatal intensive care unit. | an outbreak of bacillus cereus (b. cereus) bacteremia occurred in our neonatal intensive care unit (nicu) in july 2005. many strains of b. cereus were cultured from patient specimens, as well as from environmental samples such as the surfaces of instruments and air in the nicu. some of these strains were analyzed by pulsed field gel electrophoresis, and several were confirmed to be identical. we speculated that the bacterial load in the environment had initially increased and then possibly sprea ... | 2011 | 22038125 |
| promoting bacillus cereus spore germination for subsequent inactivation by mild heat treatment. | sublethal heat treatment may activate dormant spores and thereby potentiate the conversion of spores to vegetative cells. as the germinated spore is known to possess lower heat resistance than its dormant counterpart, it has been postulated that double heat treatment, i.e., spore heat activation followed by germination and then by heat inactivation, can be used to control spores in foods. production of refrigerated processed foods of extended durability often includes more than one heat treatme ... | 2011 | 22186048 |
| prevalence of bacillus cereus and associated risk factors in chinese-style fried rice available in the city of colombo, sri lanka. | abstract the present study aimed to assess the prevalence of bacillus cereus and its associated risk factors in chinese-style fried rice available in colombo city. in 200 samples of fried rice the prevalence of b. cereus was 56%. the prevalence by variety of fried rice was chicken (20.0%), vegetable (18.0%), seafood (10.0%), egg (5.0%), mixed (2.0%), and beef (1.0%). of analyzed samples, 28 (14%) had colony counts >10(6) colony forming units per gram (cfu/g), the infectious dose for b. cereus ... | 2011 | 22085218 |
| microbial contamination of food products consumed by infants and babies in korea. | the objectives of this study were to investigate the microbiological safety of various foods intended for consumption by infants and babies. | 2011 | 21883321 |
| bioremoval of trivalent chromium using bacillus biofilms through continuous flow reactor. | present study deals with the applicability of bacterial biofilms for the bioremoval of trivalent chromium from tannery effluents. a continuous flow reactor was designed for the development of biofilms on different substrates like glass beads, pebbles and coarse sand. the parameters for the continuous flow reactor were 20 ml/min flow rate at 30°c, ph4. biofilm biomass on the substrates was in the following sequence: coarse sand>pebbles>glass beads (4.8 × 10(7), 4.5 × 10(7) and 3.5 × 10(5)cfu/cm(2 ... | 2011 | 21924829 |
| The use of a porcine intestinal cell model system for evaluating the food safety risk of Bacillus cereus probiotics and the implications for assessing enterotoxigenicity. | The use of porcine intestinal cell lines in assessing toxicity of Bacillus cereus probiotics in conjunction with animal challenge trials with toxigenic B. cereus was investigated. Toxigenic and toxin deletion mutants of B. cereus and two probiotic strains (Paciflor and Toyocerin) were examined for bacterial attachment, cytotoxicity and ability to induce nitric oxide as markers of toxicity. Both cytotoxicity and production of nitric oxide were detected in wild-type toxigenic strains and the Pacif ... | 2011 | 22085364 |
| chitosan and silver nanoparticles as pudding with raisins with antimicrobial properties. | chitosan nanoparticles (cs-np) containing small silver nanoparticles are reported (ag@cs-np). cs-np was synthesized using tripolyphosphate (tpp) as a polyanionic template. tpp also served to electrostatically attract ag(+) inside cs-np, where it was reduced by the terminal glucosamine units of the biopolymer. this procedure is environmental friendly, inexpensive, and permits the synthesis of very small agnp (0.93-1.7 nm), with only a discrete dependence from the amount of silver nitrate used (5- ... | 2011 | 21903218 |
| protection activity of a novel probiotic strain of bacillus subtilis against salmonella enteritidis infection. | the activity of 240 bacterial isolates screened from the gastrointestinal tracts of native chickens were evaluated for use as a potential probiotic in food animal production in order to protect against animal diseases and reduce pathogenic contamination of human food products. in observing the antagonistic activity of 117 bacilli isolates, 10 of these isolates exhibited higher growth inhibition of seven foodborne pathogens, including salmonella enteritidis, salmonella typhimurium, escherichia co ... | 2011 | 21907374 |
| salt sensitivity of minimal twin arginine translocases. | bacterial twin arginine translocation (tat) pathways have evolved to facilitate transport of folded proteins across membranes. gram-negative bacteria contain a tatabc translocase composed of three subunits named tata, tatb, and tatc. in contrast, the tat translocases of most gram-positive bacteria consist of only tata and tatc subunits. in these minimal tatac translocases, a bifunctional tata subunit fulfils the roles of both tata and tatb. here we have probed the importance of conserved residue ... | 2011 | 22041895 |
| Whole-genome phylogenies of the family Bacillaceae and expansion of the sigma factor gene family in the Bacillus cereus species-group. | The Bacillus cereus sensu lato group consists of six species (B. anthracis, B. cereus, B. mycoides, B. pseudomycoides, B. thuringiensis, and B. weihenstephanensis). While classical microbial taxonomy proposed these organisms as distinct species, newer molecular phylogenies and comparative genome sequencing suggests that these organisms should be classified as a single species (thus, we will refer to these organisms collectively as the Bc species-group). How do we account for the underlying simil ... | 2011 | 21864360 |
| An analysis of temperature adaptation in cold active, mesophilic and thermophilic Bacillus a-amylases. | A comparative biochemical and structural study was performed on a cold active a-amylase from Bacillus cereus (BCA) and two well-known homologous mesophilic and thermophilic a-amylases from Bacillus amyloliquefaciens (BAA) and Bacillus licheniformis (BLA). In spite of a high degree of sequence and structural similarity, drastic variations were found for T(opt) as 50, 70 and 90°C for BCA, BAA and BLA, respectively. The half-lives of thermoinactivation were 1 and 9 min for BCA and BAA at 80°C respe ... | 2011 | 21907234 |
| A hospital acquired outbreak of Bacillus cereus gastroenteritis, Oman. | To investigate the course of a hospital acquired outbreak of Bacillus cereus gastroenteritis outbreak, and the interventions that were taken to prevent such an outbreak from occurring again. | 2011 | 22000845 |
| analytical method of cereulide in processed cereal-based foods and milk powder by lc-ms/ms. | an lc-ms/ms method for analysis of cereulide, an emetic toxin produced by bacillus cereus, was developed. cereulide was extracted from samples, fried rice, pan-fried noodles, red bean paste and baby formula, with methanol and purified using oasis hlb cartridges. lc separation was performed on a c18 column with a mixture of formic acid solution and methanol containing ammonium formate as a mobile phase, and the mass spectrometer was operated in the positive electrospray ionization mode. performan ... | 2011 | 22200747 |
| biodegradation potential of oily sludge by pure and mixed bacterial cultures. | the biodegradation capacity of aliphatic and aromatic hydrocarbons of petrochemical oily sludge in liquid medium by a bacterial consortium and five pure bacterial cultures was analyzed. three bacteria isolated from petrochemical oily sludge, identified as stenotrophomonas acidaminiphila, bacillus megaterium and bacillus cibi, and two bacteria isolated from a soil contaminated by petrochemical waste, identified as pseudomonas aeruginosa and bacillus cereus demonstrated efficiency in oily sludge d ... | 2011 | 21993328 |
| Native Valve Bacillus cereus Endocarditis in a Non-IV Drug Abusing Patient: A Case Report and Review of the Literature. | Bacillus cereus is a rare cause of endocarditis, typically associated with intravenous drug abuse, rheumatic heart disease, prosthetic heart valves, pacemakers, or immunodeficiency. We present the first case of native valve Bacillus cereus endocarditis with no apparent risk factors. The patient had a fulminant course requiring emergent valve replacement. | 2011 | 22116159 |
| Diagnostic properties of three conventional selective plating media for selection of Bacillus cereus, B. thuringiensis and B. weihenstephanensis. | The aim of this study was to assess the diagnostic properties of the two selective plating media and a chromogenic medium for identification of Bacillus cereus. The 324 isolates were B. cereus (37%), Bacillus weihenstephanensis (45%) or Bacillus thuringiensis (18%), as identified by a new combination of techniques. All isolates were growing on mannitol-egg yolk-polymyxin agar (MYP), and they did not form acid from mannitol. However, a significant lower number of B. thuringiensis isolates did not ... | 2011 | 22083787 |
| trypan blue dye enters viable cells incubated with the pore-forming toxin hlyii of bacillus cereus. | trypan blue is a dye that has been widely used for selective staining of dead tissues or cells. here, we show that the pore-forming toxin hlyii of bacillus cereus allows trypan blue staining of macrophage cells, despite the cells remaining viable and metabolically active. these findings suggest that the dye enters viable cells through the pores. to our knowledge, this is the first demonstration that trypan blue may enter viable cells. consequently, the use of trypan blue staining as a marker of ... | 2011 | 21909398 |
| Biochemical characteristics and biological properties of annurca apple cider. | Abstract Our work aimed to investigate the phenolic composition and antioxidant capacity of the de-alcoholized extract of cider obtained from the Annurca apple (Malus domestica var. Annurca). The antimicrobial effect of the extract against different pathogens, including Chronobacter sakazakii, was also examined. The extract's potential anti-quorum-sensing (AQS) activity was assessed using the test microorganism Chromobacterium violaceum. Biochemical analysis of the extract using ultra-performa ... | 2011 | 21861727 |
| microbial examination of nonheated foods served in feeding programs of elementary schools, iksan city, jeonbuk province, korea. | more than 90% of elementary school students in korea have lunch provided by a school feeding program. this study examined nonheated foods, foods in which final ingredients were added after cooking ("heated/nonheated foods"), and desserts for microbial contamination levels and the presence of foodborne pathogens. we obtained a total of 77 food samples belonging to the above three groups from four elementary schools located in iksan, jeonbuk, korea, from june to july 2010. among the samples, 15% o ... | 2011 | 21902929 |
| Efficacy of intravascular catheter lock solutions containing preservatives in the prevention of microbial colonization. | There is little published evidence regarding whether heparin lock solutions containing preservatives prevent catheter-related infections. However, adverse effects from preservative-containing flushes have been documented in neonates, leading many hospitals to avoid their use altogether. Infection control records from 1982 to 2008 at St. Jude Children's Research Hospital (SJCRH) were reviewed regarding the incidence of catheter-related infections and the use of preservative-containing intravenous ... | 2011 | 21945067 |
| [contamination by bacillus cereus on equipment and utensil surfaces in a food and nutrition service unit]. | the confirmation that bacillus cereus is a microorganism that represents a special problem in food processing plants, such as university cafeterias, inspired this work, the scope of which was to evaluate the risks consumers are exposed to by identifying the contamination points from whence the microorganism can be transferred on to food. the presence of b. cereus was detected in 38.3% of the equipment and utensils studied. counts of up to 5.7 x 10 ² cfu/cm² were found, with the highest values be ... | 2011 | 21987337 |
| defense role of the cocoon in the silk worm bombyx mori l. | silk from the domesticated silk worm bombyx mori procures foreign body response naturally, so it has been utilized as a biomaterial for decades. in india the prime focus of the sericulture industry is to improve silk production with high quality silk. naturally, the silk worm builds its cocoon not only with silk proteins, but also with antimicrobial proteins to avoid infection since the cocoon is non-motile and non-feeding. the aim of the present study is to elucidate the antimicrobial proteins ... | 2011 | 22006381 |
| phytochemical constituents and antibacterial efficacy of the flowers of peltophorum pterocarpum (dc.) baker ex heyne. | to investigate the preliminary phytochemistry and antibacterial activity of the flower extract of peltophorum pterocarpum . | 2011 | 21967698 |
| Hyicin 3682, a bioactive peptide produced by Staphylococcus hyicus 3682 with potential applications for food preservation. | Bacteriocins are peptides produced by bacteria and having inhibitory activity against other bacteria. Many of these substances may be useful as antibacterial agents for practical applications. In this study, 21 Staphylococcus spp. isolated from pigs, dogs and bovine milk in different states of Brazil were investigated for staphylococcin production. Hyicin 3682, a bacteriocin produced by one such strain, inhibited almost all strains tested, including Bacillus cereus, Listeria monocytogenes and St ... | 2011 | 22019494 |
| fluorogenic cephalosporin substrates for β-lactamase tem-1. | cephalosporin was used to synthesize soluble and precipitating fluorogenic β-lactam substrates that demonstrated differential catalytic hydrolysis by three different subtypes of β-lactamase: tem-1 (class a), p99 (class c), and a bacillus cereus enzyme sold by genzyme (class b). the most successful soluble substrate contained difluorofluorescein (oregon green 488) ligated to two cephalosporin moieties that, therefore, required two turnovers to produce the fluorescent oregon green 488 leaving grou ... | 2011 | 21867672 |
| Gram-positive bacteria on grass pollen exhibit adjuvant activity inducing inflammatory T cell responses. | Recently, it has been established that pollen grains contain Th2-enhancing activities besides allergens. | 2011 | 22092824 |
| Bovine intestinal bacteria inactivate and degrade ceftiofur and ceftriaxone with multiple beta-lactamases. | The veterinary cephalosporin drug ceftiofur is rapidly degraded in the bovine intestinal tract. A cylinder-plate assay was used to detect microbiologically active ceftiofur, and high-performance liquid chromatography-mass spectrometry analysis was used to quantify the amount of ceftiofur remaining after incubation with bovine intestinal anaerobic bacteria, which were isolated from colon contents or feces from 8 cattle. Ninety-six percent of the isolates were able to inactivate ceftiofur to some ... | 2011 | 21876048 |
| [Etiological aspects of acute gastroenteritis--a ten-year review (1.01. 2001-31.12.2010)]. | Acute gastroentritis is one of the most common diseases in humans, and continues to be a significant cause of morbidity worldwide. | 2011 | 22046776 |
| antimicrobial activities of hydrogen peroxide and its activation by a novel heterogeneous fenton's-like modified pan catalyst. | to investigate the potential activation of hydrogen peroxide by a novel catalyst, reducing the concentration of hydrogen peroxide required and the time taken for microbial inactivation. | 2011 | 21933313 |
| a new depsidone and antibacterial activities of compounds from usnea undulata stirton. | usnea undulata stirton (usneaaceae) is a fruticose lichen used locally in ethnoveterinary medicine to treat mammary infections in cattle while human beings use it for the treatment of wounds in eastern cape, south africa. bioactivity-guided fractionation of its extracts led to the isolation and characterization of a new depsidone, 2'-o-methylhypostictic acid (8), together with seven known compounds, i.e. methyl β-orsellinate (1), norstictic acid (2), menegazziaic acid (3), (+) usnic acid (4), hy ... | 2011 | 22115039 |
| bacillus cereus from blood cultures: virulence genes, antimicrobial susceptibility and risk factors for blood stream infection. | we characterized the profiles of virulence genes and antimicrobial susceptibility of bacillus cereus isolates from blood cultures as well as the risk factors for blood stream infections (bsis). the diversity of virulence gene patterns was found to be wide among 15 b. cereus isolates from bsis and also among 11 isolates from contaminated blood cultures. the microscan broth microdilution method yielded results corresponding with those of the agar dilution (reference) method for levofloxacin, linez ... | 2011 | 22077223 |
| diversity of bacterial communities that colonize the filter units used for controlling plant pathogens in soilless cultures. | in recent years, increasing the level of suppressiveness by the addition of antagonistic bacteria in slow filters has become a promising strategy to control plant pathogens in the recycled solutions used in soilless cultures. however, knowledge about the microflora that colonize the filtering columns is still limited. in order to get information on this issue, the present study was carried out over a 4-year period and includes filters inoculated or not with suppressive bacteria at the start of t ... | 2011 | 22015683 |
| fatal outcome of bacillus cereus septicaemia. | bacillus cereus is a ubiquitous environmental micro-organism which is often a contaminant of clinical cultures. infections due to b. cereus are described, but mostly in immunocompromised patients. we report a fatal outcome of b. cereus septicaemia in an immunocompetent patient with a mechanical mitral valve. | 2011 | 22173364 |
| investigation of microbial safety of a full-scale ozonation and biological activated carbon process under high humidity and temperature conditions. | microbial safety of a full-scale ozonation and biological activated carbon (bac) process was investigated by examining pathogens, microbial community and particle counts, with emphasis on the bac effluent. the process is located at south china, where the average humidity and air temperature were 70-80% and 22-24 °c, respectively. a high diversity of microbial community existed on the bac media. three types of dominant bacteria were identified, including chryseobacterium indologenes, bacillus bre ... | 2011 | 22156135 |
| poly(3-hydroxybutyrate) production by bacillus cereus spv using sugarcane molasses as the main carbon source. | the main hindrance in the use of polyhydroxyalkanoates (phas) as a replacement for existing petroleum-based plastics is their high production cost. the carbon source accounts for 50% of the cost for pha production. thus, increasing the yield and productivity of phas on cheap substrates is an important challenge for biotechnologists to support the commercialization and further applications of these polymers. in this study, we have investigated the use of an agricultural raw material, sugarcane mo ... | 2011 | 22147642 |
| chemical, antioxidant and antimicrobial investigations of pinus cembra l. bark and needles. | the chemical constituents and biological activity of pinus cembra l. (pinaceae), native to the central european alps and the carpathian mountains, are not well known. the aim of the present work was to examine the phenolic content, antioxidant and antimicrobial effects of hydromethanolic extracts of pinus cembra l. bark and needles. bark extract had higher concentrations of total phenolics (299.3 vs. 78.22 mg gallic acid equivalents/g extract), flavonoids (125.3 vs. 19.84 mg catechin equivalents ... | 2011 | 22143542 |
| chemical composition and antimicrobial activity of essential oil of achillea cretica l. (asteraceae) from turkey. | hydrodistilled volatile oil from the aerial parts of achillea cretica l. (asteraceae) was analysed by a combination of gc and gc/ms. seventy-six components were identified, constituting 86.4% of the oil. the main constituents of the essential oil were caryophylladienol-ii (13.4%), β-maaliene (6.1%), neo-intermedeol (6.0%), carvone (4.9%), spathulenol (4.5%), palmitic acid (3.3%) and selina-3,11-dien-6α-ol (3.2%). the antimicrobial activity was evaluated by the broth-dilution method on nine micro ... | 2011 | 21988483 |
| antibacterial properties of a glycolipid-rich extract and active principle from nunavik collections of the macroalgae fucus evanescens c. agardh (fucaceae). | this study investigated the antibacterial activity of glycolipid-rich extracts of the brown macroalga fucus evanescens in cell culture. accessions were collected on the arctic coast of ungava bay, nunavik, quebec. the crude ethyl acetate extract of these accessions showed strong antibacterial activity (≥4 log(10) cfu) against hemophilus influenzae , legionella pneumophila , propionibacterium acnes (atcc and clinical isolate), and streptococcus pyogenes at 100 µg/ml. this algal extract inhibited ... | 2011 | 21859295 |
| Dynamics and diversity of phosphate mineralizing bacteria in the coral reefs of Gulf of Mannar. | Phosphatase Producing Bacteria (PPB) and Inorganic Phosphate Solubilizing Bacteria (IPSB) are important to reef nutrition. These microbes and phosphate concentration play a significant role in the productivity of coral reef ecosystems. A study was conducted in Gulf of Mannar coral reef ecosystem to understand the diversity of these groups of bacteria and their competence in mineralizing the phosphate. The PPB isolates were identified under six genera i.e. Bacillus, Pseudomonas, Micrococcus, Vibr ... | 2011 | 21953521 |
| validation of arsenic resistance in bacillus cereus strain ag27 by comparative protein modeling of arsc gene product. | the ars gene system provides arsenic resistance to a variety of microorganisms and can be chromosomal or plasmid-borne. the arsc gene, which codes for an arsenate reductase is essential for arsenate resistance and transforms arsenate into arsenite, which is extruded from the cell. therefore, arsc gene from bacillus cereus strain ag27 isolated from soil was amplified, cloned and sequenced. the strain exhibited a minimum inhibitory concentration of 40 and 35 mm to sodium arsenate and sodium arseni ... | 2011 | 21258851 |
| Fluorinated s-triazinyl piperazines as antimicrobial agents. | A series of 1,3,5-triazine derivatives that contain 4-amino-2-trifluoromethyl-benzonitrile, 8-hydroxyquinoline, and different piperazines as substituents at the carbon atoms of the triazine ring have been synthesized by a simple and efficient synthetic protocol. The chemical structures of the compounds were elucidated with the aid of IR, 1H NMR and 13C NMR spectroscopy, and elemental analysis. The antimicrobial activity of the compounds was tested against seven bacteria (Staphylococcus aureus MT ... | 2011 | 21950158 |
| identification of a bacillus anthracis specific indel in the yeac gene and development of a rapid pyrosequencing assay for distinguishing b. anthracis from the b. cereus group. | bacillus anthracis, the causative agent of anthrax, is a potential source of bioterrorism. the existing assays for its identification lack specificity due to the close genetic relationship it exhibits to other members of the b. cereus group. our comparative analyses of protein sequences from bacillus species have identified a 24 amino acid deletion in a conserved region of the yeac protein that is uniquely present in b. anthracis. pcr primers based on conserved regions flanking this indel in the ... | 2011 | 21907250 |
| removal of high concentrations of nh(3) by a combined photoreactor and biotrickling filter system. | average emission levels as high as 800 ppm(v) nh(3) have often been found during the anaerobic fermentation process. at these levels, nh(3) is regarded as an environmental toxic compound. high concentrations of nh(3) gas are difficult to treat in a single treatment process, suggesting that, in terms of economic cost and treatment performance, a coupled system may be a feasible technological alternative. in the coupled tio(2) photocatalytic-biological treatment system evaluated here, the optimal ... | 2011 | 22175871 |
| a cell-cell communication system regulates protease production during sporulation in bacteria of the bacillus cereus group. | in sporulating bacillus, major processes like virulence gene expression and sporulation are regulated by communication systems involving signalling peptides and regulators of the rnpp family. we investigated the role of one such regulator, nprr, in bacteria of the bacillus cereus group. we show that nprr is a transcriptional regulator whose activity depends on the nprx signalling peptide. in association with nprx, nprr activates the transcription of an extracellular protease gene (npra) during t ... | 2011 | 21958299 |
| [Influence of predominant aerobic bacteria isolated from different healthy animals on daidzein biotransforming capacity by co-culture with different daidzein biotransforming bacteria]. | To investigate the influence of isolated predominant aerobic bacteria on daidzein biotransformation capacity by co-culture with daidzein biotransforming bacteria. | 2011 | 22097769 |
| nucleic acid recognition by tandem helical repeats. | protein domains constructed from tandem α-helical repeats have until recently been primarily associated with protein scaffolds or rna recognition. recent crystal structures of human mitochondrial termination factor mterf1 and bacillus cereus alkylpurine dna glycosylase alkd bound to dna revealed two new superhelical tandem repeat architectures capable of wrapping around the double helix in unique ways. unlike dna sequence recognition motifs that rely mainly on major groove read-out, mterf and al ... | 2011 | 22154606 |
| antibacterial activity of water-soluble extract from pine needles of cedrus deodara. | the antibacterial activity of water-soluble extract from pine needles of cedrus deodara (wec) was evaluated on five food-borne bacteria, and its related mechanism was investigated by transmission electron microscope. in vitro antibacterial assay showed that wec possesses a remarkable antibacterial activity against tested food-borne bacteria including escherichia coli, proteus vulgaris, staphylococcus aureus, bacillus subtilis and bacillus cereus, with the minimum inhibitory concentration (mic) a ... | 2011 | 22104118 |
| surface architecture of endospores of the bacillus cereus/anthracis/thuringiensis family at the subnanometer scale. | bacteria of the bacillus cereus family form highly resistant spores, which in the case of the pathogen b. anthracis act as the agents of infection. the outermost layer, the exosporium, enveloping spores of the b. cereus family as well as a number of clostridia, plays roles in spore adhesion, dissemination, targeting, and germination control. we have analyzed two naturally crystalline layers associated with the exosporium, one representing the "basal" layer to which the outermost spore layer ("ha ... | 2011 | 21896762 |
| synthesis and biological evaluation of some thiazolidinones as antimicrobial agents. | a novel series of thiazolidinone derivatives namely 4-(4-dimethylamino-6-{4-[5-(4-ethylpiperazin-1-ylmethyl)-4-oxo-2-phenylthiazolidin-3-yl]-phenylamino}-[1,3,5]triazin-2-yloxy)-1-methyl-1h-quinolin-2-one have been synthesized from the key intermediate 4-[4-(4-aminophenylamino)-6-dimethylamino-[1,3,5]triazin-2-yloxy]-1-methyl-1h-quinolin-2-one (7). condensation reaction of compound 7 with different aldehyde derivatives were performed to obtain schiff base derivatives, which after cyclization gav ... | 2011 | 22182927 |
| antimicrobial activity, cytotoxicity, and phytochemical screening of voacanga globosa (blanco) merr. leaf extract (apocynaceae). | to determine the antibacterial, antifungal, antiprotozoal, cytotoxic, and phytochemical properties of ethanol extracts of leaves of voacanga globosa (blanco) merr. (v. globosa). | 2011 | 22014741 |
| formation of cereulide and enterotoxins by bacillus cereus in fermented african locust beans. | afitin, iru and sonru are three spontaneously fermented african locust bean benin condiments. the fermentation processes are exothermic, with temperatures mostly being above 40 °c. a total of 19 predominant bacillus cereus isolates from afitin, iru and sonru, were investigated. the enterotoxin genes nhe (a, b, c) were present in all 19 isolates, the hbl (a, c, d) in one (afitin), and the cytk gene in three isolates (afitin). levels of cytotoxicity to vero cells and nhea production in bhi-broth w ... | 2011 | 21925026 |
| antibacterial activity of three medicinal thai plants against campylobacter jejuni and other foodborne pathogens. | leaves of adenanthera pavonina, moringa oleifera and annona squamosa are used in traditional thai medicine to treat dysentery and other diseases. this study investigated the antibacterial activity of these plants against six species of foodborne pathogen. methods and solvents employed to extract active constituents were optimised using the disc diffusion assay. phytochemical analysis of the optimised extracts was performed by thin layer chromatography (tlc). minimum inhibitory concentrations (mi ... | 2011 | 21878033 |
| n-acyl-homoserine lactones from enterobacter sakazakii (cronobacter spp.) and their degradation by bacillus cereus enzymes. | a chemical study of acyl-homoserine lactones (acyl-hsls) produced by e. sakazakii resulted in the identification of three molecules: (s)-n-heptanoyl-hsl, (s)-n-dodecanoyl-hsl and (s)-n-tetradecanoyl-hsl. mixed cultures of e. sakazakii and b. cereus depleted e. sakazakii acyl-hsls, suggesting acyl-hsl degradation by b. cereus hydrolases (hydrolysis of the lactone or amide moiety). the expression of b. cereus acyl-hsl lactonase and acyl-homoserine acylase was confirmed by monitoring the biotransfo ... | 2011 | 22132860 |
| complete genome sequence and characterization of the n-acylhomoserine lactone-degrading gene of the potato leaf-associated solibacillus silvestris. | n-acylhomoserine lactones (ahls) are used as quorum-sensing signal molecules by many gram-negative bacteria. we have reported that solibacillus silvestris, which was isolated from the potato leaf, has ahl-degrading activity. to identify the ahl-degrading gene, whole genome sequencing of s. silvestris stlb046 was performed by using pyrosequencing technology. as the result of the blast search, one predicted orf (ahls) showed slight similarity to aiia-like ahl lactonase from bacillus cereus group. ... | 2011 | 22019407 |
| antibacterial characteristics and activity of water-soluble chitosan derivatives prepared by the maillard reaction. | the antibacterial activity of water-soluble chitosan derivatives prepared by maillard reactions against staphylococcus aureus, listeria monocytogenes, bacillus cereus, escherichia coli, shigella dysenteriae, and salmonella typhimurium was examined. relatively high antibacterial activity against various microorganisms was noted for the chitosan-glucosamine derivative as compared to the acid-soluble chitosan. in addition, it was found that the susceptibility of the test organisms to the water-solu ... | 2011 | 21989311 |
| re-pcr variability and toxigenic profile of food poisoning, foodborne and soil-associated bacillus cereus isolates from brazil. | twenty-three bacillus cereus isolates from food poisoning outbreaks associated with a diarrheal-type syndrome, fourteen foodborne isolates not associated with food poisoning and fifteen isolates from brazilian soil samples were analyzed for the presence and genetic diversity (by re-pcr) of the virulence genes ces (emetic toxin, cereulide), plcr-papr (pleiotropic regulator plcr and peptide papr), nhea (a component of the nhe complex), bcet (diarrheal enterotoxin bc-d-ent), gyrb (b subunit of dna ... | 2011 | 21978657 |
| Validated empirical models describing the combined effect of water activity and pH on the heat resistance of spores of a psychrotolerant Bacillus cereus strain in broth and béchamel sauce. | The major objective of this study was to evaluate and model the combined effect of the water activity (a(w)) and pH of the heating menstrum on the heat resistance of spores of a psychrotolerant Bacillus cereus strain isolated from béchamel sauce. Two models, a quadratic polynomial equation and a reparameterized function, were assessed for their ability to describe the combined influence of a(w) and pH on the D(85°C)-values of the B. cereus isolate in tryptone soy broth. The performance of the m ... | 2011 | 22004813 |
| Seagrass as a potential source of natural antioxidant and anti-inflammatory agents. | Context: Halophila spp. is a strong medicine against malaria and skin diseases and is found to be very effective in early stages of leprosy. Seagrasses are nutraceutical in nature and therefore of importance as food supplements. Objective: The antibacterial, antioxidant, and anti-inflammatory activities of Halophila ovalis R. Br. Hooke (Hydrocharitaceae) methanol extract were investigated and the chemical constituents of purified fractions were analyzed. Materials and methods: Plant material ... | 2011 | 22129224 |
| developing an integrated proteo-genomic approach for the characterisation of biomarkers for the identification of bacillus anthracis. | bacillus anthracis is the causative agent of anthrax, an acute and often fatal disease in humans. due to the high genomic relatedness within the bacillus cereus group of species it is a challenge to identify b. anthracis consistently. alternative strategies such as proteomics coupled with mass spectrometry (ms) provide a powerful approach for biomarker discovery. however, validating and evaluating these markers, particularly for genetically homogeneous species such as b. anthracis are challengin ... | 2011 | 22178189 |
| brewer's spent grain: characterization and standardization procedure for the enzymatic hydrolysis by bacillus cereus strain. | an important way to reuse agroindustrial by-products and to produce added-value products consists of the production of protein hydrolysates. in the current study, we used brewer's spent grain (bsg), mainly because of its availability and cost, as a substrate for the enzymatic hydrolysis by bacillus cereus. first, the physicochemical and microbiological characterization of bsg batches from three varieties was carried out. furthermore, the optimal fermentation upstream processes for enzymatic hydr ... | 2011 | 22172109 |
| Biotransformation of puerarin into puerarin-6?-O-phosphate by Bacillus cereus. | The biotransformation of puerarin catalyzed by Bacillus cereus NT02 was studied. A primary screening was carried out using 307 strains of bacteria isolated from soil which were able to grow in the presence of puerarin. Strain NT02, identified as B. cereus, was able to convert puerarin into puerarin-6?-O-phosphate. Under the optimum conditions, resting cells of B. cereus NT02 converted 27% of added 0.4 g/l puerarin into puerarin-6?-O-phosphate that was characterized by MS, (13)C NMR, (31)P NMR. T ... | 2011 | 21882014 |
| Composition and antimicrobial activity of Seseli globiferum essential oil. | The essential oil from aerial parts of Seseli globiferum Vis. obtained by hydrodistillation with Clevenger-type apparatus was analyzed by GC-MS. Twenty-eight compounds were identified, representing 99.4% of the total oil. The main components of the oil were sabinene (38.0%), alpha-pinene (21.2%) and beta-phellandrene (13.5%). The microbial growth inhibitory properties of the isolated essential oil were determined using the broth microdilution method against seven bacterial species: Salmonella ty ... | 2011 | 21922927 |
| Monoclonal Antibodies Neutralize Bacillus cereus Nhe Enterotoxin by Inhibiting Ordered Binding of Its Three Exoprotein Components. | The Nhe enterotoxin from Bacillus cereus is known to induce cytotoxicity on Vero and CaCo-2 cells by ordered binding of its single components NheA, NheB and NheC. This study aimed to elucidate functional sites on NheB by identifying the epitopes of the neutralizing monoclonal antibodies 1E11 and 2B11. The binding regions of both antibodies were determined by using recombinant NheB fragments and synthetic peptides. The antigenic site of antibody 1E11 was located within the amino acids 321 to 341 ... | 2011 | 22104106 |
| molecular architecture of a multifunctional mcm complex. | dna replication is strictly regulated through a sequence of steps that involve many macromolecular protein complexes. one of them is the replicative helicase, which is required for initiation and elongation phases. a mcm helicase found as a prophage in the genome of bacillus cereus is fused with a primase domain constituting an integrative arrangement of two essential activities for replication. we have isolated this helicase-primase complex (bcmcm) showing that it can bind dna and displays not ... | 2011 | 21984415 |
| Impact of solvent/detergent treatment of plasma on transfusion-relevant bacteria. | Background A solvent/detergent (S/D) treatment in a medical device has been developed for pathogen reduction of plasma for transfusion. Impact of S/D on bacterial growth and on the capacity of complement to kill bacteria has been investigated in this study. Study design and methods A pool of apheresis plasma from four donors was spiked with eight transfusion-relevant bacteria. Plasma was treated with 1% tri(n-butyl) phosphate and 1% Triton X-45 at 31°C for 90 min and then extracted by oil at 3 ... | 2011 | 22092109 |
| coriander (coriandrum sativum l.) essential oil: its antibacterial activity and mode of action evaluated by flow cytometry. | the aim of this work was to study the antibacterial effect of coriander (coriandrum sativum) essential oil against gram-positive and gram-negative bacteria. antibacterial susceptibility was evaluated using classical microbiological techniques concomitantly with the use of flow cytometry for the evaluation of cellular physiology. our results showed that coriander oil has an effective antimicrobial activity against all bacteria tested. also, coriander oil exhibited bactericidal activity against al ... | 2011 | 21862758 |
| Rapid detection of vip1-type genes from Bacillus cereus and characterization of a novel vip binary toxin gene. | A PCR-restriction fragment length polymorphism (PCR-RFLP) method for identifying vegetative insecticidal protein (vip) 1-type genes from Bacillus cereus was developed by designing specific primers based on the conserved regions of the genes to amplify vip1-type gene fragments. PCR products were digested with endonuclease AciI, and four known vip1-type genes were identified. Vip1Ac and vip1Aa-type genes appeared in 17 of 26 B. cereus strains. A novel vip1-type gene, vip1Ac1, was identified from B ... | 2011 | 22092859 |
| Bacillus and relatives in foodborne illness. | Species of Bacillus and related genera have long been troublesome to food producers on account of their resistant endospores. These organisms have undergone huge taxonomic changes in the last 30 years, with numbers of genera and species now standing at 56 and over 545, respectively. Despite this expansion, relatively few new species have been isolated from infections, few are associated with food and no important new agents of foodborne illness have been reported. What has changed is our knowled ... | 2011 | 22121830 |
| Rapidly progressive, fatal, inhalation anthrax-like infection in a human: case report, pathogen genome sequencing, pathology, and coordinated response. | Ten years ago a bioterrorism event involving Bacillus anthracis spores captured the nation's interest, stimulated extensive new research on this pathogen, and heightened concern about illegitimate release of infectious agents. Sporadic reports have described rare, fulminant, and sometimes fatal cases of pneumonia in humans and nonhuman primates caused by strains of Bacillus cereus , a species closely related to Bacillus anthracis. | 2011 | 21882964 |
| Evolution and some functions of the NprR-NprRB quorum-sensing system in the Bacillus cereus group. | Quorum-sensing (QS) is a bacterial mechanism for regulation of gene expression in response to cell density. In Gram-positive bacteria, oligopeptides are the signaling molecules to elicit QS. The RNPP protein family (Rap, NprR, PlcR, and PrgX) are intracellular QS receptors that bind directly to their specific signaling peptide for regulating the transcription of several genes. NprR is the activator of a neutral protease in Bacillus subtilis, and it has been recently related to sporulation, cry g ... | 2011 | 22159892 |
| Chemical and microbiological hazards associated with recycling of anaerobic digested residue intended for agricultural use. | In the present study, three full-scale biogas plants (BGP) were investigated for the concentration of heavy metals, organic pollutants, pesticides and the pathogenic bacteria Bacillus cereus and Escherichia coli in the anaerobically digested residues (ADR). The BGPs mainly utilize source-separated organic wastes and industrial food waste as energy sources and separate the ADR into an ADR-liquid and an ADR-solid fraction by centrifugation at the BGP. According to the Norwegian standard for organi ... | 2011 | 21865025 |
| [analyze and compare metabolic pathways of bacillus cereus group]. | a large number of data and information was obtained from genome sequencing and high-throughput genomic studies, use of the information to study metabolic networks become a new hotspot in biological research. this article compared different methods to reconstruct metabolic networks and analyzed the advantages and disadvantages of each methods, and then introduced some researches about carbohydrate metabolism pathways, amino acid metabolic pathways, and energy metabolism pathways of 9 strains of b ... | 2011 | 21993280 |
| comparing nonsynergy gamma models and interaction models to predict growth of emetic bacillus cereus for combinations of ph and water activity values. | this research aims to test the absence (gamma hypothesis) or occurrence of synergy between two growth-limiting factors, i.e., ph and water activity (a(w)), using a systematic approach for model selection. in this approach, preset criteria were used to evaluate the performance of models. such a systematic approach is required to be confident in the correctness of the individual components of the combined (synergy) models. with bacillus cereus f4810/72 as the test organism, estimated growth bounda ... | 2011 | 21705525 |
| evolutionary history and functional characterization of three large genes in bacillus cereus group bacteria involved in sporulation. | the bacillus cereus group of bacteria is a group of closely related species that are of medical and economic relevance, including b. anthracis, b. cereus, and b. thuringiensis. bacteria from the bacillus cereus group encode three large, highly conserved genes of unknown function (named crda, crdb and crdc) that are composed of 16-35 copies of a repeated domain of 132 amino acids at the protein level. bioinformatic analysis revealed that there is a phylogenetic bias in the genomic distribution of ... | 2011 | 21821775 |
| polyhydroxyalkanoate (pha) synthesis by class iv pha synthases employing ralstonia eutropha phb(-)4 as host strain. | class iv polyhydroxyalkanoate (pha) synthase from bacillus cereus yb-4 (pharc(yb4)) or b. megaterium nbrc15308(t) (pharc(bm)) was expressed in ralstonia eutropha phb(-)4 to compare the ability to produce pha and the substrate specificity of pharcs. pharc(yb4) produced significant amounts of pha and had broader substrate specificity than pharc(bm). | 2011 | 21821924 |
| selection of phytotoxin producing rhizobacteria. | in order to select phytotoxin producing rhizobacteria to control weed plants, twenty five bacterial strains previously isolated from the rhizospheres of various plants were grown in a liquid medium and, after cell removal by centrifugation, the liquid phases were freeze-dried and the products were extracted with ethyl acetate/methanol. the extracts were concentrated to dryness under vacuum and dissolved in water and sucrose solution to be submitted to in vitro assays of lettuce (lactuca sativa l ... | 2011 | 21739082 |
| detection, cellular localization and antibacterial activity of two lytic enzymes of pediococcus acidilactici atcc 8042. | aim:ôçé in pediococcus acidilactici atcc 8042, two activities of peptidoglycan hydrolase (pgh) with lytic effect against micrococcus lysodeikticus and staphylococcus aureus have been detected. this work intends to elucidate the growth phase of maximum lytic activity, the localization and the effectiveness of the activity against pathogenic gram-negative and gram-positive bacteria. methods and results:ôçé cells were grown in mrs medium and collected at different growth stages, and the proteins we ... | 2011 | 21707881 |
| hygienic quality and antibiotic resistance profile of sliced butchery. | in order to investigate the microbiological quality of different meat products on the greek market, 200 samples were collected from the following preparations: boiled turkey (n=50), boiled pork ham (n=50), smoked turkey (n=50) and smoked pork ham (n=50). in all cold meat preparations clostridium perfringens vegetative and spore forms, staphylococcus aureus, escherichia coli and other clostridium sp lec(-), as well as lactobacillus, bacillus sp. and salmonella sp. were recovered. for instance bac ... | 2011 | 21722746 |