Publications

neutrophil chemotaxis induced by anaerobic bacteria isolated from necrotic dental pulps.	anaerobic bacteria belonging to the species bacteroides melaninogenicus, bacteroides asaccharolyticus, fusobacterium nucleatum, peptostreptococcus anaerobius and eubacterium alactolyticum, isolated from necrotic dental pulps, were tested for their capacity to induce neutrophil leukocyte chemotaxis. generation of chemotactic factors in serum by whole bacterial cells and chemotaxis induced by bacterial extracts were studied in vitro by the boyden technique. subcutaneously implanted wound chambers ...	1980	6103573
in vitro inhibition of neisseria gonorrhoeae growth by strict anaerobes.	thirty two anaerobic bacterial strains were screened for their in vitro inhibitory activity toward 21 neisseria gonorrhoeae strains, including two penicillin-resistant strains. from this screening, six anaerobic bacteria belonging to the genera bacteroides, peptostreptococcus, and eubacterium were selected for their large antigonococcal spectrum of activity. the inhibitory activity of these six strains appears to be specific to the gonococcus as a variety of anaerobes and aerobes were not genera ...	1980	6105130
continuous fluorescence assay, partial purification and properties of flavokinase from megasphaera elsdenii.		1980	6246392
do anaerobes cause urinary tract infection?		1980	6101823
a possible role of eubacterium and peptostreptococcus species in the aetiology of crohn's disease.	the faecal flora of patients with crohn's disease has been found to contain higher numbers of anaerobic gram-negative rods and gram-positive coccoid rods than the flora of healthy subjects. in a considerable percentage of sera from patients with crohn's disease, agglutinating antibodies were found against four strains of the coccoid rods, in contrast to sera from patients with ulcerative colitis, other diseases and healthy subjects. in the present study, coccoid strains c18, me46 and me47 were f ...	1980	7235691
introduction of anaerobic methodology into a clinical microbiological laboratory.	for the successful introduction of anaerobic methodology into a clinical microbiological laboratory, several factors are very important. these include: 1) laboratory; 2) establishment of skilful technicians and adequate equipment; and 3) communication with clinicians who believe in anaerobes as infectious agents and are interested in working with the microbiologist. the beneficial effects of these factors on a laboratory's efficiency in providing an anaerobic service are demonstrated by the foll ...	1980	7005099
pediatric infectious cervical lymphadenitis.	infectious cervical lymphadenitis is a frequently encountered problem in the pediatric population. the recent experience with infectious cervical lymphadenitis at the children's hospital of philadelphia was reviewed. the most common etiologic agent was staphylococcus aureus. beta-hemolytic streptococcus and mycobacterium were found in 15% and 6% of cases respectively. other agents included pneumococcus, cat scratch, toxoplasma gondii, actinomycosis, and mixed bacteroides and peptostreptococcus. ...	1980	6821410
the assay on a defined medium of the effects of beta-2-thienylalanine on the growth of anaerobic bacterial isolates from phenylketonuric patients.	faecal samples were taken from three diet-managed phenylketonuric children to determine effects of beta-2-thienylalanine (beta-2-t) on indigenous bacteria. from sample swabs, 127 anaerobes were identified and tested for beta-2-t inhibition on a phenylalanine (phe)-free medium, anaerobe inhibition test (ait) agar. of the isolates, 77.9% grew sufficiently to assay reactions on at least 25% of ait plates. using phe-containing columbia agar, 86.5% of the strains could be assayed. none of 28 bacteroi ...	1980	7189815
[a bacteriological study of peritonsillar abscess and its chemotherapy (author's transl)].		1980	6942141
bacterial studies of peritoneal cavity and postoperative surgical wound drainage following perforated appendix in children.	this study reports bacterial specimens obtained from 112 children presenting with a ruptured appendix. additional samples were studied from 11 of these patients who developed a postoperative surgical draining wound. bacterial growth occurred in 100 peritoneal fluid specimens. anaerobic bacteria alone were present in 14 specimens, aerobes alone in 12, and mixed aerobic an anerobic flora in 74 specimens. there were 144 aerobic isolates (1.4 per specimen). the predominant isolates were: e. coli (57 ...	1980	7406576
aerobic and anaerobic bacteriology of cervical adenitis in children.	needle aspirates from 53 inflamed cervical lymph glands were studies for aerobic and anaerobic bacteria and mycobacteria. bacterial growth was achieved in 45 patients (85%). sixty-six bacterial isolates were recovered, averaging 1.5 isolates per specimen (0.8 aerobes and 0.7 anaerobes), with as many as 4 isolates in some specimens. aerobic organisms alone were recovered in 27 aspirates (60%) of the 45 culture-positive aspirates, anaerobic bacteria alone in 8 (18%), and mixed aerobic and anaerobi ...	1980	7408372
['sterile' brain abscesses (author's transl)].	in 16 cases of brain abscess (1970 to 1976) 10 could be identified to be bacteriologically sterile. however in 5 cases of "sterile" cerebral abscesses, where swabs were taken under anaerobic conditions, it was possible to isolate anaerobic bacteria in every case. it is concluded that in reality thse so-called "sterile" brain abscesses were also contaminated with anaerobes.	1980	7415618
agglutinins to bacteria in crohn's disease.	sera from patients with crohn's disease were tested for antibodies against organisms which are thought to cause inflammatory bowel disease in animals, or have been implicated in human crohn's disease. control sera were collected from healthy individuals and patients with ulcerative colitis. sera from crohn's disease and controls failed to agglutinate clostridium colinum or campylobacter sputorum subsp. mucosalis and two strains of mycobacterium paratuberculosis (m26 and m27). most of the sera ag ...	1980	7429299
[pleuro-pulmonary infections with anaerobic germs (author's transl)].	the author presents his experience with the study of 42 patients with pleuro-pulmonary infection with anaerobic germs. he underlines the value of research tending to improve the conditions of sampling and culturing to ensure a bacteriological diagnosis and the setting of a suitable therapy.	1980	7433351
anaerobic and aerobic bacteriology of decubitus ulcers in children.	forty-two children with decubitus ulcers were studied using aerobic and anaerobic techniques. anaerobic bacteria were isolated in 21 (50%) of the patients, five times as the only isolates and 16 times mixed with aerobes. aerobes only were present in 20 (48%) of the patients. there were a total of 83 isolates, 46 aerobes and 37 anaerobes, with an average of two species per specimen (1.1 aerobes and 0.9 anaerobes). the predominant anaerobic isolates were gram-positive cocci (17), bacteroides fragi ...	1980	7436139
[saprophytic and opportunistic non spore-forming anaerobic microflora of the vagina (author's transl)].	a microbiological survey has been carried out on 179 healthy, child-bearing aged, non-pregnant women, with the aim to evaluate the incidence of anaerobic non-spore forming bacteria in the normal vaginal flora. this group of bacteria has been isolated in 50.3% of women, with a clear prevalence of "anaerobic streptococci " and bacteroides, followed by fusobacterium and veillonella. no propionibacterium, eubacterium or bifidobacterium have been isolated. according to many authors the non-spore form ...	1980	7018415
kinetic parameters of lactate dehydrogenases of some rumen bacterial species, the anaerobic ciliate isotricha prostoma and mixed rumen microorganisms.	a number of kinetic parameters of the lactate dehydrogenases of three rumen bacterial species (peptostreptococcus productus, propionibacterium acnes and actinomyces viscosus), the rumen ciliate isotricha prostoma and mixed rumen microorganisms (mrm) with respect to nadh, pyruvate, fructose-1,6-diphosphate (fdp) as well as the effects of several nucleotide phosphates were studied. partially purified ldh of peptostr. productus had the same kinetic parameters as in crude cell free extracts. values ...	1980	6778389
puerperal sepsis in port moresby, papua new guinea.	because puerperal sepsis is the second most common cause of maternal death in papua new guinea some aspects of its aetiology and prevention have been investigated. 91% of 125 women with puerperal fever were found to have post-partum uterine infection. the anaerobes bacteroides and peptostreptococcus singly or together, were the commonest causes, while streptococcus pyogenes was the predominant aerobic species. antibiotic sensitivity patterns were established, and chloramphenicol was found to be ...	1980	7008426
antigenic relationships and rapid identification of peptostreptococcus species.	antisera against whole cells of each peptostreptococcus species (p. anaerobius, p. micros, p. parvulus, and p. productus) were produced in rabbits. when these antisera were reacted against sonically disrupted cells and culture supernatant fluids in ouchterlony tests, lines of identity were obtained among the antigens from all the species and uninoculated culture medium. when the antisera were subsequently absorbed with the dehydrated culture medium used to grow the peptostreptococci, all cross-r ...	1980	6769956
microorganisms associated with abscesses of sheep and goats in the south of iran.	a total of 86 abscesses (45 and 41) abscesses of sheep and goats, respectively) were examined for their causal agents; 44 of these abscesses were located subcutaneously, and the remaining 42 were in lungs, livers, intestines, and udders. a total of 23 different types of microorganisms were isolated from 78 abscesses; bacteria were not detected in the remaining eight abscesses. microorganisms isolated were: species of the genera corynebacterium, staphylococcus, and streptococcus and pasteurella, ...	1980	6996545
determination of fmn and fad by fluorescence titration with apoflavodoxin.		1980	7374468
production of p-hydroxyhydrocinnamic acid from tyrosine by peptostreptococcus anaerobius.	peptostreptococcus anaerobius was found to metabolize tyrosine to p-hydroxyhydrocinnamic acid [3-(p-hydroxyphenyl)propionic acid]. this acid was detected in spent growth media by gas-liquid chromatography, and its identity was confirmed by mass spectrometry.	1980	7229012
bactericidal effect of hydrogen peroxide is prevented by the lactoperoxidase-thiocyanate system under anaerobic conditions.	streptococcus sanguis and peptostreptococcus anaerobius were exposed to various combinations of the components of the lactoperoxidase-thiocyanate-hydrogen peroxide system. the bactericidal effect of hydrogen peroxide was prevented under anaerobic conditions by lactoperoxidase together with thiocyanate, but not by lactoperoxidase or thiocyanate alone. thiocyanate was effective already at a molar ratio to hydrogen peroxide of 1:100.	1980	7429633
effect of temperature, humidity and exposure to oxygen on the survival of anaerobic bacteria.	the relative effect of humidity, temperature and exposure to oxygen on the survival of 11 strains of bacteriodes spp., fusobacterium necrophorum, eubacterium lentum, clostridium ramosum, peptococcus asaccharolyticus and peptostreptococcus anaerobius was evaluated. suspensions of the test strains were applied to membrane filters either in empty sterile petri dishes or on plates of non-nutrient agar to provide dry or moist conditions. the filters were stored aerobically or anaerobically in an atmo ...	1980	7431377
[bacteriological examinations of liquid drawn from the pouch of douglas (author's transl)].	the article reports on bacteriological examinations of 510 samples of liquid drawn from the pouch of douglas (excavatio rectouterina) during pelviscopy in patients not displaying any signs of inflammation. bacterial populations were found in 270 samples (= 52.9%). the results are subdivided according to aerobic and anaerobic types of pathogens and their incidence.	1980	7450495
isolation, synthesis, and properties of 8-hydroxyflavins.		1980	7374472
[pathology caused by anaerobic bacteria in pediatrics. our cases].		1980	7453681
[endocarditis due to the anaerobe peptostreptococcus].		1980	7450583
improved procedure for transport of dental plaque samples and other clinical specimens containing anaerobic bacteria.	an improved transport system for samples containing anaerobic bacteria was developed. this system increased the recovery rate of anaerobic bacteria up to 28.8% as compared to a commonly used method.	1979	39087
the presence of antibody-coated anaerobic bacteria in asymptomatic bacteriuria during pregnancy.	quantitative anaerobic culture of urine samples obtained from 593 pregnant women by suprapubic bladder aspiration was performed to establish the involvement of anaerobic bacteria in asymptomatic urinary tract infections. the fluorescent antibody (fa) test was applied to the sediments of bladder aspirates to determine the site of infection. anaerobic bacteriuria (greater than or equal to 10(4) microorganisms/ml of urine) was found in 34 patients, of whom five were fa-positive. these anaerobes wer ...	1979	118996
identification of bacteroides fragilis by indirect immunofluorescence.	rabbit antiserum against live or heat-killed bacteroides fragilis nctc 9343 bacteria was titrated against hot phenol water-extracted polysaccharide antigens from five different species of the 'b. fragilis group' of bacteria using an enzyme immunoassay and shown to be specific for the b. fragilis nctc 9343 polysaccharide. when the antiserum was used in indirect immunofluorescence, 97.1% of 244 b. fragilis strains were correctly identified. only 8 of the other 312 bacteroides strains were stained ...	1979	94651
post-tonsillectomy anaerobic septicemia.		1979	286176
anaerobic and aerobic bacteriology of acute conjunctivitis.	aerobic and anaerobic cultures and clinical data were obtained from 131 patients presenting with acute conjunctivitis. similar cultures were obtained from 60 noninflamed individuals. anaerobes were isolated from 66 patients, 51 times in mixed culture with aerobes and 15 times (11.5%) as the only isolates. aerobes only were recovered in 54 patients. the organisms found to be statistically significantly more commonly recovered from eyes with conjunctivitis were staphylococcus aureus, hemophilus in ...	1979	313179
[bacteriological aspects of trichomonal vaginitis (author's transl)].	an analysis was made of the vaginal flora of 25 gynaecological patients with acute trichomomal vaginitis, with the view to elucidating the bacteriological situation. eighty-four isolates, an average of 3.4 per patient, were taken and included a wide range of aerobic and anaerobic bacteria. - obligate anaerobic species, such as bacteriodes and peptostreptococci, with susceptibility to metronidazole were among the predominant pathogens. - the differentiated susceptibility of the most common bacter ...	1979	386649
the prevalence of anaerobic bacteria in suprapubic bladder aspirates obtained from pregnant women.	the incidence of asymptomatic bacteriuria due to aerobic, facultative anaerobic and strictly anaerobic bacteria during pregnancy was determined in 185 patients admitted for complications of pregnancy, using suprapubic bladder aspiration. anaerobic bacterial counts of 103 per ml urine or higher were detected in 6.4 per cent of the specimens. the incidence of anaerobic bacteriuria seems comparable to that of bacteriuria due to aerobic and facultative anaerobic micro organisms. in our group of pati ...	1979	388942
[technics for isolation, incubation and preliminary identification tests].	in this report the aa. emphasize the fact that anaerobes are mostly isolated from mixed cultures in which several bacteria are present. therefore they suggest to grow all the bacteria under the best conditions and consequently to select the anaerobes from the aerobes. furthermore they describe the selecting techniques used at istituto superiore di sanità and clinica medica iii, to attain this aim and enumerate the best methods used for anaerobic incubation.	1979	399835
extractable antigen shared by peptostreptococcus anaerobius strains.	extracts from several species of gram-positive cocci were prepared by a modification of the rantz-randall autoclave method and tested for reactions with rabbit anti-peptostreptococcus anaerobius (atcc 27337 and vpi 5737) sera in a capillary precipitin test. antigen preparations from two reference strains of p. anaerobius (atcc 27337 and vpi 5737) and six clinical isolates of p. anaerobius reacted with the p. anaerobius antisera. these extracts formed a line of identity by immunodiffusion and dis ...	1979	88461
[short-term oral chemoprophylaxis before intestine surgery. quantitative determination of bacteria and fungi in stool specimens (author's transl)].	the methods of quantitative analysis of aerobe and anaerobe microbes and fungi stool specimens are described. the results of the studies in health people are compared to the results in patients undergoing surgical treatment of intestinal tract. a group of these patients received neomycin and bacitracin orally as short-time chemoprophylaxis to diminish possible woundinfection and/or sepsis. after oral medication germs as bifidobacterium, bacteroides and clostridium (not cl. perfringens) are reduc ...	1979	452770
bactericidal effect of cysteine exposed to atmospheric oxygen.	peptostreptococcus anaerobius vpi 4330-1 was exposed to atmospheric oxygen in a dilution bland (0.2% gelatin, salts, resazurin) solution. the organisms were rapidly killed when the solution contained cysteine. the organisms were effectively protected by catalase and horseradish peroxidase as well as by the metal ion-chelating agents 8-hydroxyquinoline and 2,2'-bipyridine. superoxide dismutase increased the rate of killing of the organisms, whereas singlet oxygen quenchers and scavengers of hydro ...	1979	453819
isolation and characterisation of bacteria from abscesses in the subcutis of cats.	thirty-six closed abcesses in the subcutis of cats were examined. of 168 bacterial strains isolated, 121(72%) were anaerobes and 47 (28%) were facultative anaerobes. twenty-six abscesses contained mixtures of facultative anaerobes and anaerobes, six contained anaerobes only and four contained facultative anaerobes only. bacteriodes was the genus most commonly isolated (28.6% of all isolates) followed by fusobacterium (19.0%) and pasteurella (multocida) (13.1%). peptostreptococcus anaerobius was ...	1979	458840
[first steps in the bacterial colonization of the digestive tract of neonates (author's transl)].	meconium samples obtained from 14 children aged 18 to 51 h were subjected to a quantitative differential analysis. the meconium of one of the children (47 h old) was sterile; in those of the 11 infants aged less than 47 h the dominant flora was composed of either enterobacteria, mainly escherichia coli sensitive to usual antibiotics, or of streptococci. the genus acinetobacter constituted the dominant flora in one case only. staphylococcus, corynebacterium, clostridium (c. perfringens), bacteroi ...	1979	475219
description of strains of peptostreptococcus anaerobius isolated from subcutaneous abscesses in cats.	strains of peptostreptococcus, streptococcus and of a gram-positive coccus, which was initially isolated as an anaerobe but grew subsequently as a facultative organism, were isolated from subcutaneous abscesses in cats. the cat strains of peptostreptococcus gave metabolic fermentation products in combinations described for p. anaerobius. the streptococcus strains conformed to the group s. intermedius. the facultative organism described had the metabolic products of p. anaerobius but the distinct ...	1979	479840
urease-producing species of intestinal anaerobes and their activities.	urease activities of anaerobic bacteria that constituted predominant gut flora were examined. it was demonstrated that some strains of eubacterium aerofaciens, e. lentum, and peptostreptococcus products produced urease. they were the most numerous species in human feces. all strains of bifidobacterium infantis and some strains of bacteroides multiacidus, b. bifidum, clostridium symbiosum, fusobacterium necrophorum, f. varium, lactobacillus fermentum, peptococcus asaccharolyticus, and p. prevotii ...	1979	36839
aerobic and anaerobic bacterial flora in semen from fertile and infertile groups of men.	semen samples were collected by masturbation under asceptic conditions from men who had sired children within the past 6 mo (group a) and asymptomatic men attending an infertility clinic who had not sired children and whose wives were asymptomatic for infertility (group b). these 109 semen samples were analyzed and cultured for isolation of aerobic and anerobic organisms. overall, 68% of the specimens had positive bacterial cultures: 54% of the samples from group a were positive and 73% from gro ...	1979	485650
quantitative bacteriology of the vaginal flora in vaginitis.	vaginal flora was studied quantitatively in 29 sexually active women, 16 to 33 years of age, to define the role of c. vaginale in vaginitis. seventeen were asymptomatic and 12 complained of symptoms of vaginitis. seven asymptomatic women had scant secretions: four of these seven had c. vaginale at log10 6 to 9 cfu per milliliter, none of whom had "clue" cells; none had trichomonas or candida; six had lactobacilli at log10 7 to 9.7 per milliliter; only one had bacteroidaceae at greater than log10 ...	1979	420269
treatment of obstetric and gynecologic infections with cefamandole.	cefamandole nafate is a derivative of 7-aminocephalosporanic acid which has been shown to have good in vitro activity against aerobes traditionally susceptible to cephalosporins as well as many anaerobes, including b. fragilis. one hundred women with obstetric or gynecologic infections completed treatment with cefamandole: 53 had post-cesarean section infections: 24, acute pelvic inflammatory disease: 16, posthysterectomy cuff cellulitis/abscess; and seven, vulvar or abdominal wound abscess. alm ...	1979	426015
medium for selective isolation of fusobacterium nucleatum from human periodontal pockets.	a selective medium, cve agar, was developed for the isolation of fusobacterium nucleatum from subgingival plaque of periodontally diseased patients. the medium contained 1.0% trypticase (bbl microbiology systems), 0.5% yeast extract, 0.5% nacl, 0.2% glucose, 0.02% l-tryptophan, 1.5% agar, and 5% defibrinated whole sheep blood. erythromycin and crystal violet were added as the selective inhibitory agents at concentrations of 4 and 5 micrograms/ml, respectively. the medium permitted almost total r ...	1979	521483
differentiation of peptococcus and peptostreptococcus by gas-liquid chromatography of cellular fatty acids and metabolic products.	gas-liquid chromatographic (glc) profiles of cellular fatty acids and metabolic products were useful in identifying strains of peptococcus saccharolyticus, peptococcus asaccharolyticus, peptostreptococcus anaerobius, peptostreptococcus micros, and streptococcus intermedius. the glc results supported the recent taxonomic decision to transfer aerotolerant peptostreptococcus species to the genus streptococcus. because inconsistencies in the results prevented our differentiating peptococcus prevotii ...	1979	528680
long-term effects of tetracycline on the subgingival microflora.		1979	287678
bovine superoxide dismutase and copper ions potentiate the bactericidal effect of autoxidizing cysteine.	when cysteine is oxidized by oxygen, hydrogen peroxide is formed, and hydrogen peroxide is very toxic to peptostreptococcus anaerobius vpi 4330-1. native and inactivated superoxide dismutase increased the rate of oxidation of cysteine and thereby potentiated the toxic effect of cysteine. a similar increase in the rate of oxidation of cysteine and in the toxicity of cysteine was obtained with cu2+.	1979	573589
occurrence of anaerobic bacteria in diseases of the dog and cat.	a survey for anaerobic bacteria was conducted in 314 clinical specimens from dogs and cats. a total of 187 anaerobic isolates in pure and mixed culture were isolated from 111 of the specimens that contained anaerobic bacteria. common isolated included actinomyces (9.1%), clostridium perfringens (19.3%), other clostridium spp (11.2%), peptostreptococcus anaerobius (7.5%), bacteroides melaninogenicus (13.4%), other bacteroides spp (17.6%), and fusobacterium necrophorum (5.3%). anaerobic bacteria w ...	1979	224739
bacteriology of chronic otitis media.	tympanocentesis was performed in 50 patients with chronic otitis media; pus was cultured aerobically and anaerobically. only aerobes were isolated from 21 patients (42%), three patients had only anaerobes (peptococcus sp), and 25 patients (50%) had both aerobes and anaerobes. only one specimen had no growth. there were 68 aerobic isolates. pseudomonas aeruginosa was recovered in 36 patients (72%); other aerobes commonly recovered included proteus sp and staphylococcus aureus. there were 48 anaer ...	1979	759663
tyrosine degradation in presumptive identification of peptostreptococcus anaerobius.	a new tyrosine medium was developed and evaluated for the differentiation of peptostreptococcus anaerobius from other anaerobic, gram-positive cocci. the strains included 159 originating from clinical specimens and 13 reference strains received from other workers in the field. only one strain of each species was included in the study from multiple cultures from the same patient. this medium is simple to prepare and can be used in a small clinical laboratory. one hundred seventy-two strains of an ...	1979	379031
antimicrobial activity of antituberculosis agents against anaerobic bacteria.	anaerobic infections may coexist with tuberculosis, and can be mistaken for one another. the effect of therapy with antituberculosis chemotherapeutic agents against anaerobic bacteria (with the exception of rifampin) is unknown. we therefore examined the in vitro efficacy of certain commonly used antituberculosis agents (rifampin, isoniazid, and ethambutol) against 370 strains of anaerobic bacteria, including 86 isolates of bacteroides fragilis. rifampin at a concentration of 2 microgram/ml inhi ...	1979	436485
recovery of anaerobic bacteria from pediatric patients. a one-year experience.	during 1975, 115 speciments from the soft tissues, body fluids, and purulent sites in 105 pediatric ward and nursery patients were submitted to the clinical laboratory for anaerobic study, and 75% of the cultures were positive. in most instance specimens were collected in anaerobic transport tubes containing peptone yeast extract and glucose. in the laboratory, samples were processed by the anaerobic holding jar method. nineteen different anaerobic organisms were isolated; the average number of ...	1979	495592
practical technique for quantitating anaerobic bacteria in tissue specimens.	quantitative bacteriology has considerable influence on the care and management of surgical wounds. heretofore, these techniques have been limited to measurements of aerobic bacteria. a technique is reported herein which permits quantitation of obligately anaerobic bacteria in tissue specimens. this technique is easily reproduced in any clinical laboratory and eliminates the need for expensive anaerobic chambers.	1979	385618
anaerobic isolates in chronic suppurative otitis media.		1979	538681
aerobic and anaerobic bacterial flora of the maternal cervix and newborn gastric fluid and conjunctiva: a prospective study.	aerobic and anaerobic cultures were performed on cervical swabs from 35 mothers and their newborn infants' gastric aspirates and conjunctival sacs. four hundred seventy-nine isolates were obtained; 287 were aerobes and 192 were anaerobes. the cervical and gastric cultures overall yielded a similar flora, but this was not always the case when one compared mothers with their own newborn infants. the conjunctival cultures yielded about half of the number of bacteria per specimen that the gastric co ...	1979	440845
[infections by anaerobic sporeless bacteria].		1979	551585
bacteriology and therapy of lung abscess in children.	the bacteriology and clinical findings of ten pediatric patients with lung abscess are presented. bacteriologic data were based on percutaneous transtracheal aspiration obtained before initiation of antimicrobial therapy. anaerobic bacteria were present in all ten patients; in nine they were mixed with aerobic bacteria. the most frequent isolates were peptostreptococcus (8), peptococcus (5), bacteroides melaninogenicus (6), and bacteroides fragilis (3). the aerobic isolates most frequently recov ...	1979	758385
perirectal abscess in childhood. a review of 29 cases.	a total of 29 cases of perirectal abscess at milwaukee children's hospital between 1965 and 1975 were retrospectively reviewed. one fourth of these children had a serious underlying chronic disease. staphylococcus aureaus was the organism that grew most frequently from pus obtained from the abscesses, accounting for 52% of the isolates. therapy included incision and drainage alone and incision and drainage plus parenteral and/or oral antibiotic treatment. one patient, a child with leukemia, died ...	1979	373420
bacterial isolates in cholecystitis and cholelithiasis.		1979	386479
metronidazole therapy of anaerobic bacteremia, meningitis, and brain abscess.	four patients with bacteroides fragilis bacteremia, one patient with a brain abscess due to bacteroides species, fusobacterium naviforme, and peptostreptococcus species, and an infant with bacteroides species ventriculitis and meningitis were treated with metronidazole. in all cases the anaerobic pathogens were eradicated. five of the six patients recovered. one patient with leukemia in whom b fragilis bacteremia was eradicated by metronidazole treatment subsequently died of pseudomonas aerugino ...	1979	434970
fecal bacteria in south african rural blacks and other population groups.	quantitative studies were performed on fecal flora of three population groups consuming different diets. twenty rural black south africans and 22 japanese, representing groups at low risk for carcinoma of the colon, were compared with 41 north americans from a high-risk population. specimens taken immediately after defecation were mixed and processed under anaerobic conditions. after the initial incubation, roll tubes were shipped to the united states for final identification. bacterioides and b ...	1979	447500
[bacteriological diagnosis of anaerobic infections (author's transl)].	a review on anaerobic bacteria with special reference to their role in human disease and an outline for clinical laboratory practice (collection of the specimens, culture media, isolation, identification, antibiotic sensitivity tests) is presented.	1979	485599
capacity of anaerobic bacteria from necrotic dental pulps to induce purulent infections.	combinations of bacteria isolated from the root canals of teeth with necrotic pulps and periapical bone destruction were tested for their capacity to induce abscess formation and transmissible infections when inoculated subcutaneously into guinea pigs. transmissible infections could be induced with combinations obtained from teeth with purulent apical inflammation, but not with combinations from symptomless teeth with chronic apical inflammation. all combinations which gave transmissible infecti ...	1979	489126
anaerobic bacteria in late infections following orthopedic surgery.	twelve patients with late anaerobic infection following hip or knee arthroplasty or tibia osteosynthesis are presented. peptococci (peptococcus asaccharolyticus, p. magnus, p. prevotii) and peptostreptococci (peptostreptococcus micros, p. anaerobius, p. parvulus) were present in nine of the cases. anaerobic gram-positive or -negative rods were found in three cases (propionibacterium acnes, actinomyces israelii, and bacteroides fragilis). antibodies against the isolated bacteria were detected in ...	1979	492071
subdural interhemispheric empyema in a 7-year-old boy.	subdural interhemispheric empyema was diagnosed by angiography and computerized tomography (ct) in a 7-year-old boy suffering from acute left hemiparesis. after neurosurgical intervention his condition improved. peptostreptococcus intermedius was cultured from the empyema. one year after hospitalisation the boy is in good condition. the importance of the ct-scanning for diagnosis and management of brain abscess or empyema is stressed.	1979	541221
comparison of three methods of susceptibility testing of bacteroidaceae, peptococcaceae and other anaerobes to doxycycline.	the in vitro activity of doxycycline against 147 strains of gram-positive and gram-negative anaerobes was determined by broth dilution, agar dilution and agar diffusion tests. the strains were isolated from clinical specimens in 1977. saccharolytic bacteroides strains (39 b. fragilis, 6 b. thetaiotaomicron, 4 b. vulgatus) showed broth dilution mics of less than or equal to 0.0625-4.0 microgram/ml after 5 h incubation and mics of less than or equal to 16 microgram/ml after 15 h incubation at 37 d ...	1979	543353
osteomyelitis of the mandibular condyle secondary to dental extractions.	although osteomyelitis of the mandibular condyle secondary to dental extraction is scarcely mentioned in the medical and dental literature, we have seen three cases within a two-year period. the first two patients were examined for an eroded condyle three months and 18 months following the extractions. the third patient, who was treated one month after extraction for acute osteomyelitis of the condyle, has done well. all three patients required operations and administration of long-term antibiot ...	1979	503487
obligate anaerobes in clinical veterinary practice.	clinical specimens obtained from domestic animals were examined to determine the relative prevalence of obligate anaerobic bacteria and the species represented. of 3,167 samples cultured anaerobically as well as aerobically, 2,234 were bacteriologically positive. of these positive samples, 583 (26%) contained species of obligate anaerobic bacteria in a total of 641 isolates. most positive samples contained anaerobes admixed with aerobic species, although 6% of such samples yielded pure cultures ...	1979	511987
factors related to the oxygen tolerance of anaerobic bacteria.	the effect of atmospheric oxygen on the viability of 13 strains of anaerobic bacteria, two strains of facultative bacteria, and one aerobic organism was examined. there were great variations in oxygen tolerance among the bacteria. all facultative bacteria survived more than 72 h of exposure to atmospheric oxygen. the survival time for anaerobes ranged from less than 45 min for peptostreptococcus anaerobius to more than 72 h for two clostridium perfringens strains. an effort was made to relate th ...	1978	697363
enhancement of experimental anaerobic infections by blood, hemoglobin, and hemostatic agents.	certain foreign materials have been demonstrated to enhance the infectivity of aerobic and anaerobic bacteria. whole blood and other protein compounds encountered in surgical settings or trauma were tested for their effect on infectivity of nonsporeforming anaerobic bacteria. infectious synergistic mixtures of bacteroides fragilis plus peptostreptococcus anaerobius and bacteroides melaninogenicus plus fusobacterium necrophorum were each diluted to a barely noninfectious or minimally infectious c ...	1978	631883
recurrent acute suppurative thyroiditis.	two patients had two recurrent episodes each of acute suppurative thyroiditis. circulating concentrations of plasma thyrotrophin, thyroxine, and triiodothyronine were within the range of normal. antibodies to thyroid microsome or thyroglobulin were not found. anaerobic bacteria such as bacteroides, peptostreptococcus, and peptococcus were identified as causative agents, indicating that anaerobic organisms may play an important role in the pathologic process. the present cases are the third and f ...	1978	717311
microbiota of gingivitis in man.	a study on the predominant cultivable microorganisms inhabiting gingival crevices affected with a chronic gingivitis was carried out using the roll tube culture technique. samples were obtained from nine individuals 25--42 years of age. gram-positive rods make up 29.1% of the isolates and included mainly actinomyces naeslundii, actinomyces israelii, and actinomyces viscosus. streptococcus mitis and streptococcus sanguis together made up 26.8% of the cultivable organisms. peptostreptococcus avera ...	1978	276916
peptostreptococcal endophthalmitis with a relapsing course.	a posttraumatic exogenous endophthalmitis developed that was due to peptostreptococcus. this gram-positive anaerobe was isolated in pure culture from vitreous. the endophthalmitis showed a bacteriologic and clinical relapse despite conventional routes of antimicrobial treatment. a second clinical relapse occurred despite apparent vitreal sterilization with intravitreal antibiotics. the response to vitrectomy was impressive, and postoperatively the patient's visual acuity improved to 20/20.	1978	697615
[anaerobiosis in purulent material].		1978	714804
antimicrobial effect of amniotic fluid.	the antimicrobial effect of amniotic fluid (af) obtained during the first (af1) and second (af2) trimesters was compared with the third (af3) against anaerobic bacteria, such as bacteroides fragilis ss. fragilis (6 strains), eubacterium lentum (3 strains), and peptostreptococcus anaerobius (4 strains). escherichia coli (5 strains) served as a positive control. af1 supported the growth of all 4 anaerobes (except b fragilis for 4 hours) for the entire 24-hour period tested. af2 supported the growt ...	1978	355963
comparative susceptibilities of anaerobic bacteria to metronidazole, ornidazole, and sc-28538.	the susceptibilities of 284 anaerobic bacteria, including 55 strains of the bacteroides fragilis group, were determined by an agar dilution technique to metronidazole and two newer nitroimidazoles, ornidazole and sc-28538. all three agents showed marked in vitro activity against virtually all anaerobic bacteria tested. at concentrations </=1 mug/ml, sc-28538 was significantly more active than either metronidazole or ornidazole. at concentrations of >1 mug/ml, the activities of all three agents w ...	1978	718156
ticarcillin disodium in anaerobic infections.	twenty-five patients were treated with ticarcillin disodium, 18 of whom had anaerobic infections that included pleuropulmonary infections (seven), mandibular osteomyelitis (four), perirectal abscess (two), sepsis, primary site unknown (one), liver abscess (one), pelvic abscess (one), decubitus ulcer (one), and synergistic gangrene (one). seven had no anaerobic infections. three had anaerobic septicemia. culture results included anaerobes: peptococci (ten), peptostreptococci (ten), bacteroides fr ...	1978	718311
effect of silver nitrate application on the conjunctival flora of the newborn: and the occurrence of clostridial conjunctivitis.	newborn conjunctival cultures were obtained from 35 babies prior to silver nitrate application and 48 hours later. on initial culture, 46 facultative bacteria and 27 anaerobes were recovered; 48 facultative and 18 anaerobes were recovered after 48 hours. haemophilus vaginalis, bacteroides species and anaerobic cocci decreased in numbers, whereas s. epidermidis, micrococcus and propionibacterium acnes increased during this time interval. clostridial species were isolated from two cases who develo ...	1978	216788
clindamycin-induced enterocolitis in hamsters.	a lethal enterocolitis was induced in hamsters by oral or parenteral administration of clindamycin in amounts comparable to those used in treatment of humans. the intestinal lesions were characterized histologically as an acute inflammatory reaction with pseudomembrane formation and resembled the lesions seen in humans with antibiotic-induced colitis. results of quantitative stool cultures showed the numbers of peptostreptococcus and corynebacterium decreased in animals with colitis after challe ...	1978	649990
anaerobic bacteremia as observed in a children's hospital. clinically this may signify true anaerobic sepsis.	to ascertain the significance of anaerobic bacteremia in a children's hospital, the records of all patients whose blood cultures grew anaerobes during a 24-month period were reviewed. anaerobes were isolated from 144 out of 1,126 blood cultures yielding bacteria. anaerobic diphtheroid grew in 122 out of 143 anaerobic cultures, but only 4 out of 122 were isolated from patients with anaerobic sepsis. nine per cent of the total episodes of anaerobic bacteremia occurred in 13 children who met out cr ...	1978	679583
anaerobic microflora of the vagina in children.	vaginal cultures from 25 healthy girls from two months to 15 years of age were examined for aerobic, facultatively anaerobic, and obligately anaerobic bacteria. an average of 8.7 species (3.7 aerobic and facultatively anaerobic species; 5.3 obligately anaerobic species) were isolated from these cultures. staphylococcus epidermidis (21), diphtheroids (20), bacteroides (19), peptococci (19), peptostreptococci (14), and bacteroides melaninogenicus (14) were most prevalent organisms.	1978	686083
nutritional characteristics of megasphaera elsdenii.	megasphaera elsdenii b159 (formerly peptostreptococcus elsdenii; rogosa 1971) is able to grow in a chemically defined medium containing minerals, nh4+ as the sole nitrogen source, sulfate as the source of sulfur, acetate and glucose as the carbon and energy sources, and the vitamins biotin, pyridoxine, and calcium pantothenate. when lactate serves as the carbon source, with cysteine present as the reducing agent, acetate is not required. valine and threonine appeared to be required although dire ...	1978	688105
[various antigenic components in peptococcus and peptostreptococcus strains].		1978	694106
the bacteriology of cavitating pulmonary infections and empysema. studies of transtracheal aspirates and pleural fluid.	a prospective study of bacteriology in 14 cases of cavitating pulmonary infections and empyema is reported. bacteriologic results were based on transtracheal aspirates or pleural fluid. appropriate anaerobic bacteriologic methods were employed. anaerobic bacteria were recovered in 11 patients (79%); they were the only pathogens isolated in 6 patients. the predominant species were fusobacterium nucleatum, bacteroides melaninogenicus, bacteroides fragilis and peptostreptococcus. aerobic bacteria w ...	1978	352421
acute suppurative parotitis caused by anaerobic bacteria: report of two cases.	staphylococcus aureus is the predominant pathogen in acute suppurative parotitis. although anaerobic bacteria are prevalent in the normal oral and upper respiratory tract flora and frequently are involved in oral and facial infections, they have seldom been isolated from patients with suppurative parotitis. it may be that a role for anaerobes in parotitis has not been documented because of inadequate specimen transport or anaerobic culture techniques. we describe two cases of acute suppurative p ...	1978	32513
hydrogen peroxide and superoxide radical formation in anaerobic broth media exposed to atmospheric oxygen.	fourteen different broth media were autoclaved under anaerobic conditions and then exposed to atmospheric oxygen. the hydrogen peroxide and superoxide radical formation as well as the bactericidal effect of the media were studied. the rate of killing of peptostreptococcus anaerobius vpi 4330-1 was high in media that rapidly autoxidized and accumulated hydrogen peroxide. in actinomyces broth (bbl), 50% of the cells were killed within 2 min, and in brewer thioglycolate medium (difco), 50% were kil ...	1978	29560
serological reactions of the genus peptostreptococcus.	white male new zealand rabbits were immunized with soluble antigen preparations (sp) of the following gram-positive anaerobic cocci: peptostreptococcus anaerobius atcc 27337 and vpi 5737; p. micros vpi 2618; streptococcus morbillorum atcc 27527; p. parvulus vpi 5229; and p. productus atcc 27340. sp were reacted with homologous and heterologous rabbit antisera in immunodiffusion, immunoelectrophoresis, indirect fluorescent-antibody, and tanned-cell passive hemagglutination tests. even though each ...	1978	357444
effects of storage in an anaerobic transport system on bacteria in known polymicrobial mixtures and in clinical specimens.	an anaerobic transport system (ats) which provides for catalytic removal of oxygen was evaluated by using in vitro-prepared polymicrobial mixtures of logphase bacteria and clinical specimens. inoculated swabs were stored at room temperature in (i) aerobic, (ii) anaerobic glove box, and (iii) ats environments, and bacteria were quantitated after 2, 24, 48, and 72 h. bacteria in a three-part mixture of bacteroides fragilis, peptostreptococcus anaerobius, and escherichia coli and in a five-part mix ...	1978	370142
[sensitivity of anaerobes in gyneco-obstetric infections].		1978	232934
[surgical infections by anaerobes in gyneco-obstetrics].		1978	232933
metastatic peptostreptococcus intermedius endophthalmitis after a dental procedure.	a 46-year-old man developed symptoms of a chronic progressive uveitis in his right eye approximately one week after a dental procedure. the patient's intraocular inflammation was not diminished by massive treatment with topical and systemic corticosteroid therapy or intravenously administered adrenocorticotropic hormone. the inflammatory process progressed to an overt endophthalmitis during a period of three weeks and the eye eventually required evisceration. a pure culture of peptostreptococcus ...	1978	306760
dental caries in gnotobiotic rats incubated with a strain of peptostreptococcus intermedius.		1978	280581
dental caries in gnotobiotic rats inoculated with a strain of peptostreptococcus intermedius.		1978	277560
rubredoxin.		1978	213684
a new hydrolase specific for taurine-conjugates of bile acids.	through the investigation of the bile acid-deconjugation activities of human intestinal anaerobes, a new enzyme was discovered in peptostreptococcus intermedius which hydrolyzed specifically the taurine-conjugates, but not the glycine-conjugates of bile acids. however, the enzymes in streptococcus faecalis and lactobacillus brevis hydrolyzed chiefly the glycine-conjugates.	1978	100491
quantitative evaluation of three commercial blood culture media for growth of anaerobic organisms.	the ability of three different commercial blood culture media--brain heart infusion broth (pfizer), thiol broth (difco), and prs-peptone broth (becton, dickinson & co.)--to support the growth of five different anaerobes is described. inocula of 100 and 1,000 colony-forming units per ml were used to evaluate potential differences in survival, lag time, growth rate, and doubling times of each anaerobe in each medium. in addition, each medium was evaluated for its ability to neutralize the antibact ...	1978	24059
predominant cultivable microbiota in periodontosis.		1977	138729