Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [movement of gastric acid secretion and influence of proton pump inhibitors on histamine h2 receptor antagonist resistant ulcer]. | treatment of peptic ulcers has become easier with the advent of h2 blockers. however, h2 resistant ulcer still remain. these cases were screened by 24 hr-intragastric ph monitoring, which well reflects the status of gastric acid secretion. on the one hand, there were cases in which no nocturnal elevation in gastric ph was seen even after administration of h2 blocker (h2 blocker resistant ulcer). on the other hand, there were cases which were resistant to treatment with h2 blocker, although the g ... | 2006 | 1347323 |
| the measurement of radiant temperature in neonatal thermal environments. | heat exchange through radiation is recognized as the dominant mode of heat transfer for an infant nursed in an incubator or warmer. a radiometer was constructed to measure the planar radiant temperature experienced by the infant. two heat-flow sensors of different emissivities were mounted onto a heat sink such that one measured principally convective heat exchange while the other measured convective and radiant heat exchange. the radiant heat exchange was obtained from the difference between th ... | 2006 | 1393209 |
| a 3-year evaluation of the tcu380ag and the cu-7. | the tcu380ag and the cu-7 intrauterine devices (iuds) were evaluated in a randomized comparative clinical trial. a total of 198 women who had not recently been pregnant entered the trial in manila, philippines and were followed-up through 3 years postinsertion. the 36-month follow-up rates were impressively high at 80.3 for tcu 380a users and 76.7 for users of the cu-7. although the two iuds demonstrated statistically similar termination rates, the findings suggest a higher efficacy for the tcu3 ... | 2006 | 2564357 |
| [the significance of the determination of catecholamines and their metabolites in neuroblastomas]. | a method for the determination of catecholamines and their metabolites in the urine was established. the specimens were previously cleaned by ion exchangers and the components were separated by a 'reversed phase' ionic pair chromatography, determined by an electrochemical 'wall-jet'-detector. this method was applied in order to find a diagnostic marker in case of neuroblastoma diseases. in 85 per cent of the patients (n = 13) we stated an increased ejection of dopamine. | 2006 | 2095056 |
| [ataxia telangiectasia]. | 2006 | 1610280 | |
| [ca15-3 tumor marker in early detection of breast cancer]. | ca15-3 is a tumor marker associated with mammary tumors. increased levels have been observed in patients with breast cancer, while normal low levels are usually found in women with no evidence of disease. the potential clinical uses of this marker include monitoring of patients with breast cancer, prognosis, and early detection of recurrence. tumor markers are not usually used for diagnosis, as only in a few patients are elevated levels found. but when high levels of tumor markers are detected a ... | 2006 | 1563679 |
| cyclosporine provides better success rates for both higher- and lower-risk cadaveric kidney transplant recipients. | 2006 | 3291212 | |
| the general practice contract scheme: was it targeted? | to determine whether the contracted general practices were situated in areas of greatest health need. | 2006 | 1538862 |
| subdural empyema complicating frontal and ethmoid sinusitis. | 2006 | 2564381 | |
| a role of carbohydrate-carbohydrate interaction in the process of specific cell recognition during embryogenesis and organogenesis: a preliminary note. | a possible role of cell surface glycoconjugates in cell recognition has been envisioned based on recognition of carbohydrates by cell surface proteins such as endogenous lectins, glycosyltransferases, and hydrolases (refs. 18-22 in text). a new possibility that a specific carbohydrate at the cell surface could be recognized by the same or similar carbohydrate on the counterpart cell surface is now suggested by specific interaction between lex and lex, but not between lex and sialylated or non-su ... | 2006 | 2563943 |
| [nursing under a different sky: sweden]. | 2006 | 1489594 | |
| [optimal method of the hypotensive treatment of hypertension with diuretics and sodium chloride substitutes]. | the therapeutic efficacy of combined and separate application of a diuretic (brinaldix) and beta-adrenoblocker (visken) was compared in the treatment of 69 patients with essential hypertension running a stable course; the patients were kept on a low-sodium diet (4-6 g nacl daily) and given a potassium substitute for nacl. | 2006 | 4087657 |
| hallermann-streiff syndrome. | 2006 | 1428149 | |
| antimicrobial prophylaxis for fractured base of skull in children. | the details are reviewed of 50 children who were treated over a 10-year period with clinical signs of fractured base of skull. two patients died early without signs of sepsis--due to the severity of their head injuries. of the remainder, 23 received antibiotic prophylaxis and 25 did not. one patient from each of these groups developed pneumococcal meningitis, and they were successfully treated. our results correlate well with those previously published, confirming the low incidence of infective ... | 2006 | 1393186 |
| accessory adrenal: an incidental finding during orchiopexy. | 2006 | 2870456 | |
| article of shaham and coworkers ("methadone maintenance" volume 28, number 3, 1991) | 2006 | 1526758 | |
| effect of postdischarge surveillance on rates of infectious complications after cesarean section. | decreases in length of stay for surgical procedures and increased outpatient surgery affect accuracy of surgical wound infection rates. to assess accuracy of rates for infectious complications after delivery by cesarean section, we implemented postdischarge surveillance at our hospital (4800 annual deliveries). physician questionnaires were used. response rate was greater than 90%. during the 5 months before postdischarge surveillance the overall infection rate was 1.6%; afterward the rate incre ... | 2006 | 1326238 |
| acute myocardial infarction after blunt chest trauma in young people: need for prompt intervention. | we describe four cases of acute myocardial infarction in young patients, secondary to blunt chest trauma. one case was treated with intracoronary thrombolysis and angioplasty, two cases received systemic thrombolysis, and the last one did not have any reperfusion therapy. the coronary angiograms of the left anterior descending artery showed thrombosis in two cases, coronary dissection in one case, and no morphological lesions in the other. we encourage the early performance of angiographic studi ... | 2006 | 1764738 |
| transcatheter management of pseudoaneurysms complicating pancreatitis. | hemorrhage from rupture of an arterial pseudoaneurysm is a potentially fatal complication of pancreatitis. seventeen patients underwent transcatheter embolization of 23 arteries for the treatment of 20 arterial pseudoaneurysms secondary to pancreatitis. their records were reviewed retrospectively to evaluate the clinical benefit of transcatheter therapy. at presentation, 15 of the 17 patients had gastrointestinal, intrasplenic, retroperitoneal, intraperitoneal, or postoperative wound bleeding. t ... | 2006 | 1797219 |
| transcatheter management of pseudoaneurysms complicating pancreatitis. | hemorrhage from rupture of an arterial pseudoaneurysm is a potentially fatal complication of pancreatitis. seventeen patients underwent transcatheter embolization of 23 arteries for the treatment of 20 arterial pseudoaneurysms secondary to pancreatitis. their records were reviewed retrospectively to evaluate the clinical benefit of transcatheter therapy. at presentation, 15 of the 17 patients had gastrointestinal, intrasplenic, retroperitoneal, intraperitoneal, or postoperative wound bleeding. t ... | 2006 | 1797219 |
| [fundus plication with or without proximal selective vagotomy?]. | the aim of this study was to determine the influence of proximal gastric vagotomy on the outcome after fundoplication. of 141 patients operated on for reflux disease between 1972 and 1988, 53 had fundoplication alone (group a) and 88 fundoplication combined with proximal gastric vagotomy (group b). the two groups were similar with regard to the severity of the reflux disease (esophagitis: a 69%, b 73%) but had a different incidence of concomitant ulcer disease (a 2%, b 55%). after a mean follow- ... | 2006 | 1621082 |
| carbohydrate specificity of the receptor sites of mistletoe toxic lectin-i. | the carbohydrate specificity of mistletoe toxic lectin-i (ml-i) was studied by haemagglutination-inhibition assay. the results indicated that ml-i has a broad range of affinity for gal alpha,beta linked sequences. the galabiose (e, gal alpha 1----4gal) sequence, a receptor of the uropathogenic e. coli ligand, was one of the best disaccharide inhibitors tested. the lectin also exhibits affinity for lac(gal beta 1----4glc), t(gal beta 1----3galnac), i/ii(gal beta 1----3/4glcnac) and b(gal alpha 1- ... | 2006 | 1525184 |
| "raynaud's disease" in migraineurs: one entity or two? | two cases are reported of patients who were diagnosed as having both migraine and raynaud's disease. because they were able to achieve effective control of both headaches and extremity temperature after undergoing a comprehensive approach--utilizing medication, biofeedback, and psychotherapy--it is suggested that both conditions are manifestations of the same disorder. it is recommended that a first step in approaching concurrent diagnoses of this type involve combined treatment as is often used ... | 2006 | 1446993 |
| comparison of magnetic resonance imaging in alzheimer's disease, vascular dementia and normal aging. | mri scans of 27 patients with probable alzheimer's disease (mean age 68.2 years), 31 patients with vascular dementia (mean age 69.9 years) and 18 normal controls (mean age 66.3 years) were compared to evaluate possible distinguishing parenchymal abnormalities among these groups. atrophy was quantitated by subjective rating, linear and volumetric measurements. a number of findings were significantly more common in vascular dementia than in the other subsets. these included (1) basal ganglionic/th ... | 2006 | 1592074 |
| better right than quick? | 2006 | 1538635 | |
| acrylic resin finger prosthesis. | a technique is described for the prosthetic replacement of two fingers using acrylic resin. retention was obtained using finger rings. the stages of the technique are outlined. advantages and disadvantages are briefly stated. | 2006 | 1484252 |
| selective iga deficiency associated with glomerulonephritis and oligoarthritis. | a 59 year old woman with selective iga deficiency associated with oligoarthritis and glomerulonephritis is described. she was seropositive for rheumatoid factor and renal histological examination showed a focal glomerulonephritis. high titre rheumatoid factor and a focal glomerulonephritis were also present in the only other well documented report of selective iga deficiency and renal disease. histological examination of the kidney suggested that the glomerulonephritis was mediated by immune com ... | 2006 | 1540017 |
| ventriculopleural shunting used as a temporary diversion. | due to the limited absorptive capacity of the pleural cavity, infants and young children are not generally ideal candidates for ventriculopleural shunts. we report using chest cavities as alternate for temporary diversion of csf in a young child. venous access to the cervical region could not be utilized because of scarring from previous procedures, while peritoneal access was contraindicated due to repeated pseudocyst formation. pleural effusions were removed by thoracentesis when necessary, an ... | 2006 | 1595400 |
| [nurses and professional secret]. | 2006 | 3645907 | |
| [nurses and professional secret]. | 2006 | 3645907 | |
| proceedings of the 3rd north sea conference on periodontology. maastricht, the netherlands, 16-19 may 1990. | 2006 | 1679767 | |
| [eye foreign bodies. patients should be sent to the eye clinic for treatment]. | 2006 | 1636298 | |
| study of acute intraoperative suprachoroidal hemorrhage. | acute intraoperative suprachoroidal hemorrhage or effusion (aish) was studied in a prospective manner over the past three years. in a general cataract surgery population of 2,523 patient eyes, 15 (0.6%) experienced an aish. results of a specific surgical management technique are presented. surgical control of the aish consisted of immediate closure of the incision and application of pressure directly to the eye. this tamponades the effusion or hemorrhage, which allows coagulation and completion ... | 2006 | 1403754 |
| radiotherapy of malignant melanoma of the uvea with i-125 seeds. | nineteen patients with malignant uveal melanomas were treated with i-125 applicators. there were 10 males and 9 females with a median age of 61 years (range 42-76). the tumour was located in the choroid in 12 eyes and in the choroid and ciliary body in 7 eyes. the size of the tumours was 7-18 mm in maximal basal diameter (median 12), 5-16 mm in minimal basal diameter (median 10), and 5.5-15 mm in thickness (median 8.5). the volume of the tumours was 123-1890 mm3 (median 540). all tumours were cl ... | 2006 | 1488887 |
| latex anaphylaxis during spinal surgery in children with myelomeningocele. | 2006 | 1489751 | |
| 16th annual symposium on computer applications in medical care. proceedings. baltimore, maryland, november 8-11, 1992. | 2006 | 1482848 | |
| [pharmacokinetic interaction of drugs with cardiac glycosides; which are clinically important?]. | 2006 | 2870436 | |
| stress and graves' disease. | 2006 | 1346682 | |
| [nursing--a woman's job!]. | 2006 | 1489626 | |
| dielectric spectroscopy as a novel and convenient tool for the study of the shear sensitivity of plant cells in suspension culture. | plant cell suspensions of different species and different age were subjected to hydrodynamic stress while following the decline in the volume fraction of intact cells by measuring the permittivity of the cell suspension at radio frequencies. results were compared with the fresh weight, dry weight, packed cell volume and cell number of the suspensions. at first a rapid decline is seen as the most shear-sensitive cells are broken up, followed by a slower decline as less sensitive cells are broken ... | 2006 | 1367236 |
| correlations between laser flare measurements and anterior chamber protein concentrations. | the laser flare cell meter quantifies anterior chamber (ac) protein (flare) by measuring light scattering of a helium-neon laser beam in the ac. the relationship between photon count and protein concentration both in vitro and in vivo was assessed. the reproducibility of the in vitro photon count measurements was 7.3%. there was a significant linear relationship between photon count and the concentration of both albumin (r = 1.0, p = 0.0001) and immunoglobulin g (igg, r = 0.99, p = 0.0001) in vi ... | 2006 | 1526738 |
| the use of potassium-titanyl-phosphate laser for laparoscopic removal of ovarian endometrioma. | from january 1989 through january 1990, 31 patients thought to have ovarian endometrioma at ultrasonography were treated by operative laparoscopy and potassium-titanyl-phosphate laser. the endometriomas ranged from 2 to 9.2 cm in diameter by preoperative transvaginal ultrasonography. the operative procedure used was a three-puncture technique with a 600 micron fiber attached to a potassium-titanyl-phosphate laser. the power setting was 10 w with a continuous pulse. in all cases endometriomas wer ... | 2006 | 1828651 |
| the use of potassium-titanyl-phosphate laser for laparoscopic removal of ovarian endometrioma. | from january 1989 through january 1990, 31 patients thought to have ovarian endometrioma at ultrasonography were treated by operative laparoscopy and potassium-titanyl-phosphate laser. the endometriomas ranged from 2 to 9.2 cm in diameter by preoperative transvaginal ultrasonography. the operative procedure used was a three-puncture technique with a 600 micron fiber attached to a potassium-titanyl-phosphate laser. the power setting was 10 w with a continuous pulse. in all cases endometriomas wer ... | 2006 | 1828651 |
| predicting the distance for gavage tube placement in children. | nurses currently use several external measures referenced to the head and chest to predict the insertion distance for orogastric and nasogastric (ng) tubes. the ability of previously published regression equations on height to predict esophageal length for ng tube insertion was evaluated in a sample of 107 children. | 2006 | 1574363 |
| ossification of the posterior cervical longitudinal ligament. | forty patients with cervical myelopathy due to opll (ossification of the posterior longitudinal ligament) of the cervical spine were studied. according to abe's or yamamoto's classification, 12 of them had a 50% decrease in the cross-sectional area of the spinal canal. subtotal vertebrectomy was carried out in 8 of these patients and the remaining 4 patients received posterior decompression. we concluded that anterior decompression, if possible, is the treatment of choice and posterior decompres ... | 2006 | 1595391 |
| a temporal-abstraction system for patient monitoring. | resume is a system that performs temporal abstraction of time-stamped data. resume is based on a model of three temporal-abstraction mechanisms: point temporal abstraction (a mechanism for abstracting values of several parameters into a value of another parameter); temporal inference (a mechanism for inferring sound logical conclusions over a single interval or two meeting intervals); and temporal interpolation (a mechanism for bridging nonmeeting temporal intervals). making explicit the knowled ... | 2006 | 1482852 |
| flunarizine in migraine: a double-blind placebo-controlled study (in a saudi population). | we evaluated the effect of flunarizine (fz) (10 mg/d) on migraine in a double-blind placebo-controlled design. the attacks' frequency, duration, severity and associated symptoms were compared before and after treatment. forty-two patients completed a three-month trial period; 21 patients received fz and 21 placebo. statistical analysis showed no significant difference between fz and placebo (p > 0.05). in this study fz was not more efficient than placebo in migraine. | 2006 | 1446992 |
| neopterin measured in serum and tissue culture supernates by a competitive enzyme-linked immunosorbant assay. | in this solid-phase competitive enzyme-linked immunosorbant assay for neopterin (a product of activated macrophages) in serum or supernates of cell cultures, we incubate antiserum to neopterin with standards or samples in the presence of solid-phase-bound conjugate of carrier-neopterin. incubation with second antibody labeled with peroxidase then follows, before reaction with substrate. analytical recovery, precision, and sensitivity of this method are similar to those of other immunoassays. the ... | 2006 | 2667801 |
| participation of health workers, school teachers and pupils in the control of rheumatic fever: evaluation of a training programme. | in a rural community block of north india we initiated a programme for control of rheumatic fever and rheumatic heart disease (rf/rhd). this included a training campaign for all 74 health workers, 773 school teachers and 12,500 older pupils (class v to x) to enable them to suspect and refer cases of rf/rhd and counsel them about secondary prophylaxis. training material was used by project staff, medical officers and teachers to convey that this serious disease with onset between 5 and 15 years c ... | 2006 | 1428137 |
| mass culture of mammalian cells as a national project. | 2006 | 1367250 | |
| a postero-anterior cephalometric evaluation of adult open bite subjects as related to normals. | reduced nasal airflow can be the result of enlarged adenoids, hypertrophic turbinates, severely deviated nasal septum, or even narrow nasal cavities. the question of whether the width of the nasal cavity may influence certain facial dimensions examined on the postero-anterior cephalometric x-ray was evaluated through the study of two different groups of subjects. possible intercorrelations between some other frontal dimensions were also examined in these subjects. the first group consisted of 15 ... | 2006 | 1748190 |
| nutrition and head start. | 2006 | 1458961 | |
| [lipid metabolism disorders: diagnosis and treatment. 4: drug therapy and dietary recommendations. national cholesterin-initiative]. | 2006 | 1855762 | |
| [lipid metabolism disorders: diagnosis and treatment. 4: drug therapy and dietary recommendations. national cholesterin-initiative]. | 2006 | 1855762 | |
| a role for nmr in alcoholism? | 2006 | 1796991 | |
| a role for nmr in alcoholism? | 2006 | 1796991 | |
| serum autoantibodies in patients with alzheimer's disease and vascular dementia and in nondemented control subjects. | in this study we sought to evaluate the clinical significance of serum autoantibodies to dementing processes. | 2006 | 1636181 |
| rubber-specific ige in children with spina bifida: identification and management. | 2006 | 1489746 | |
| m net: a statewide referring physician computer network. | m net, the referring physician computer network, is a joint project between the university of michigan medical center (ummc) and ibm. a graphical user interface was developed to allow referring physicians easy access to a variety of patient, clinical, and institutional data. file transfer mechanisms were created and current clinical database files and programs were enhanced and restructured to facilitate remote data transmission. m net is currently installed in referring physician office sites a ... | 2006 | 1482996 |
| mao inhibitors and coronary artery surgery: a patient death. | the mechanisms of action of monoamine oxidase inhibitors (maois) suggest that patients taking them may respond with hyper- or hypotension when undergoing coronary artery surgery. we describe a case where maois were present and fentanyl and midazolam were the anaesthetic agents used. the anaesthesia and surgery were performed without incident. postoperative icu care was complicated by hypertension, hyperthermia, and severe shivering followed by hypotension resistant to therapy and finally death. ... | 2006 | 1464133 |
| [the hemostatic system in traumatic subarachnoid hemorrhage in the far north]. | it was found that in conditions of the extreme north sanation of the cerebrospinal fluid occurs later in patients with traumatic subarachnoidal hemorrhage than in the central regions of the country. in connection with this the hemostasis system was studied in 20 patients with traumatic subarachnoidal hemorrhage and 10 patients with brain tumours (control group). patients with traumatic subarachnoidal hemorrhage showed significant hypocoagulation changes. | 2006 | 1448989 |
| comparing and optimizing diagnostic tests: an information-theoretical approach. | an ideal method for assessing performance of non-binary diagnostic tests would specify each test's optimal operating point and would tell a diagnostician which of many tests was the best one to use in a particular clinical situation. this article shows how information theory and receiver operating characteristic (roc) analysis can be combined to evaluate and compare diagnostic tests at their optimum cutoffs once disease prevalence and test properties are specified. though it is not appropriate f ... | 2006 | 1513208 |
| proteins of photosystem 1 during chloroplast assembly and senescence in festuca pratensis. | 2006 | 1426570 | |
| health checks on patients 75 years and over in nottinghamshire after the new gp contract. | to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners. | 2006 | 1393076 |
| [evaluation of the effect of proton pump inhibitors on stomal ulcer]. | four reports on proton pump inhibitors related to the clinical effects on stomal ulcer were reviewed. two reports were on omeprazole and the other two on lansoprazole, carried out in the pre-marketing stage. they are compared with two reports on h2-receptor antagonists (famotidine and ranitidine), which were also done in the pre-marketing stage. it appears that the proton pump inhibitors bring more rapid ulcer healing than h2-receptor antagonists without severe side effects, and there seems to b ... | 2006 | 1347326 |
| inhibition site of dexamethasone on extravasation of polymorphonuclear leukocytes in the hamster cheek pouch microcirculation. | a video system was used to investigate the inhibitory effect(s) of the glucocorticoid dexamethasone (dex) on polymorphonuclear leukocyte (pmn) extravasation in the microcirculation of hamster cheek pouch. dex was given intraperitoneally at 0.1 or 0.3 mg/kg body weight 2 h before induction of extravasation by topical application of leukotriene b4 (ltb4) or formyl-methionyl-leucyl-phenylalanine (fmlp) on the microvasculature. the number, time course, and behavior of pmns were examined in the follo ... | 2006 | 1326021 |
| the cellular and molecular biology of membrane transport. aps conference. orlando, florida, november 4-7, 1992. abstracts. | 2006 | 1360685 | |
| inhibitory effect of circulating insulin on glucagon secretion during hypoglycemia in type i diabetic patients. | to clarify whether the circulating insulin level influences hormonal responses, glucagon secretion in particular, during hypoglycemia in patients with insulin-dependent (type i) diabetes. | 2006 | 1346593 |
| aphb influences acid tolerance of vibrio vulnificus by activating expression of the positive regulator cadc. | a mutant of vibrio vulnificus that was more sensitive to low ph was screened from a library of mutants constructed by random transposon mutagenesis. by use of a transposon-tagging method, an open reading frame encoding a lysr homologue, aphb, was identified and cloned from v. vulnificus. the deduced amino acid sequence of aphb from v. vulnificus was 80% identical to that reported from v. cholerae. a mutational analysis demonstrated that the gene product of aphb contributes to acid tolerance of v ... | 2006 | 16952939 |
| genetic distinctions among clinical and environmental strains of vibrio vulnificus. | vibrio vulnificus causes rare but frequently fatal septicemia associated with raw oyster consumption by persons with underlying hepatic or immune system dysfunction. the virulence potential of environmental reservoirs appears widely distributed, because most strains are virulent in animal models; however, several investigations recently demonstrated genetic divergence among strains from clinical versus environmental origin at independent genetic loci. the present study used pcr to screen dna pol ... | 2006 | 16957239 |
| vibrio vulnificus infection and metalloprotease. | vibrio vulnificus is ubiquitous in aquatic environments; however, it occasionally causes serious and often fatal infections in humans. these include invasive septicemia contracted through consumption of raw seafood, as well as wound infections acquired through contact with brackish or marine waters. in most cases of septicemia, the patients have underlying disease(s), such as liver dysfunction or alcoholic cirrhosis, and the secondary skin lesions including cellulitis, edema and hemorrhagic bull ... | 2006 | 16958802 |
| development of monoclonal antibodies for simple identification of vibrio alginolyticus. | the present study was aimed to produce monoclonal antibodies (mabs) for simple and specific identification of vibrio alginolyticus infection in shrimp. | 2006 | 16965376 |
| vibrio vulnificus wound infections after contact with the baltic sea, germany. | 2006 | 16966781 | |
| transcriptional regulatory cascade for elastase production in vibrio vulnificus: luxo activates luxt expression and luxt represses smcr expression. | vibrio vulnificus causes diseases through actions of various virulence factors, including the elastase encoded by the vvpe gene. through transposon mutagenesis of v. vulnificus, vvpe expression was shown to be increased by luxo mutation. since the vvpe gene is known to be positively regulated by smcr via direct binding to the vvpe promoter, the role of luxo in smcr expression was investigated. the luxab-transcriptional fusions containing different lengths of the smcr promoter region indicated th ... | 2006 | 16971386 |
| cutaneous injury and vibrio vulnificus infection. | 2006 | 16972360 | |
| validation of a green fluorescent protein-labeled strain of vibrio vulnificus for use in the evaluation of postharvest strategies for handling of raw oysters. | in this paper we describe a biological indicator which can be used to study the behavior of vibrio vulnificus, an important molluscan shellfish-associated human pathogen. a v. vulnificus atcc 27562 derivative that expresses green fluorescent protein (gfp) and kanamycin resistance was constructed using conjugation. strain validation was performed by comparing the gfp-expressing strain (vv-gfp) and the wild-type strain (vv-wt) with respect to growth characteristics, heat tolerance (45 degrees c), ... | 2006 | 16980431 |
| identification of ompu of vibrio vulnificus as a fibronectin-binding protein and its role in bacterial pathogenesis. | vibrio vulnificus is a pathogenic bacterium that causes gastroenteritis and primary septicemia. to identify factors involved in microbial adherence to the host cells, we investigated bacterial proteins capable of binding to fibronectin, one of the main components comprised of the extracellular matrix of mammalian cells. a protein of approximately 35 kda was purified from the extracts of v. vulnificus by its property to bind to immobilized fibronectin. this protein was identified as ompu, one of ... | 2006 | 16988233 |
| lysine decarboxylase expression by vibrio vulnificus is induced by soxr in response to superoxide stress. | lysine decarboxylase expression by vibrio vulnificus, which is up-regulated by cadc in response to acid stress, is also induced by soxr in response to superoxide stress. soxr binds to the promoter region of the cadba operon, coding for a lysine-cadaverine antiporter (cadb) and a lysine decarboxylase (cada). the induction of cadba transcription by soxr is independent of cadc. cadaverine, which neutralizes the external medium, also appears to scavenge superoxide radicals, since increasing cellular ... | 2006 | 17012399 |
| two type iv pili of vibrio parahaemolyticus play different roles in biofilm formation. | vibrio parahaemolyticus rimd2210633 has two sets of type iv-a pilus genes. one set is similar to that found in other gram-negative bacteria, such as pseudomonas aeruginosa, vibrio cholerae (chitin-regulated pilus; chirp), and vibrio vulnificus. the other is homologous to the genes for the mannose-sensitive hemagglutinin (msha) pilus. in this study, we analyzed the effects of the deletions in the pilin genes for each type iv pilus (the chirp and the msha pilus) on biofilm formation. although the ... | 2006 | 17020553 |
| vibrio vulnificus bacteremia associated with chronic lymphocytic leukemia, hypogammaglobulinemia, and hepatic cirrhosis: relation to host and exposure factors in 252 v. vulnificus infections reported in louisiana. | vibrio vulnificus infection in persons with b-chronic lymphocytic leukemia (b-cll) or hypogammaglobulinemia has been reported infrequently. | 2006 | 17031249 |
| in situ and in vitro gene expression by vibrio vulnificus during entry into, persistence within, and resuscitation from the viable but nonculturable state. | isolation of vibrio vulnificus during winter months is difficult due to the entrance of these cells into the viable but nonculturable (vbnc) state. while several studies have investigated in vitro gene expression upon entrance into and persistence within the vbnc state, to our knowledge, no in situ studies have been reported. we incubated clinical and environmental isolates of v. vulnificus in estuarine waters during winter months to monitor the expression of several genes during the vbnc state ... | 2006 | 16461698 |
| genetic variation in the vibrio vulnificus group 1 capsular polysaccharide operon. | vibrio vulnificus produces human disease associated with raw-oyster consumption or wound infections, but fatalities are limited to persons with chronic underlying illness. capsular polysaccharide (cps) is required for virulence, and cps expression correlates with opaque (op) colonies that show "phase variation" to avirulent translucent (tr) phenotypes with reduced cps. the results discussed here confirmed homology of a v. vulnificus cps locus to the group 1 cps operon in escherichia coli. howeve ... | 2006 | 16484211 |
| quorum sensing in vibrios: complexity for diversification. | n-acylhomoserine lactone-dependent quorum sensing was first discovered in two luminescent marine bacteria, vibrio fischeri and vibrio harveyi. the luxi/r system of v. fischeri is the paradigm of gram-negative quorum-sensing systems; however, it is not found in all vibrios. a more complex quorum-sensing regulation is found in v. harveyi. three parallel systems transmit signals via phosphorelays that converge onto one regulatory protein luxo. components of the three systems are found only in vibri ... | 2006 | 16487746 |
| aminoacyl-transferases and the n-end rule pathway of prokaryotic/eukaryotic specificity in a human pathogen. | the n-end rule relates the in vivo half-life of a protein to the identity of its n-terminal residue. primary destabilizing n-terminal residues (nd(p)) are recognized directly by the targeting machinery. the recognition of secondary destabilizing n-terminal residues (nd(s)) is preceded by conjugation of an nd(p) residue to nd(s) of a polypeptide substrate. in eukaryotes, ate1-encoded arginyl-transferases (r(d,e,c*)-transferases) conjugate arg (r), an nd(p) residue, to nd(s) residues asp (d), glu ... | 2006 | 16492767 |
| specificity of a vibrio vulnificus aminopeptidase toward kinins and other peptidyl substrates. | recently, phosphoglucose isomerase with a lysyl aminopeptidase (pgi-lysap) activity was identified in vibrio vulnificus. in this paper, we demonstrate the proteolytic cleavage of human-derived peptides by pgi-lysap of v. vulnificus using three approaches: (i) a quantitative fluorescent ninhydrin assay for free lysine, (ii) matrix-assisted laser desorption ionization-two-stage time of flight mass spectrometry (maldi-tof-tof), and (iii) tricine gel electrophoresis. pgi-lysap hydrolyzed bradykinin, ... | 2006 | 16513735 |
| cyclo(phe-pro) modulates the expression of ompu in vibrio spp. | vibrio vulnificus was found to produce a chemical that induced the expression of vibrio fischeri lux genes. electron spray ionization-mass spectrometry and 1h nuclear magnetic resonance analyses indicated that the compound was cyclo(l-phe-l-pro) (cfp). the compound was produced at a maximal level when cell cultures reached the onset of stationary phase. sodium dodecyl sulfate-polyacrylamide gel analysis of the total proteins of v. vulnificus indicated that expression of ompu was enhanced by exog ... | 2006 | 16513751 |
| in situ gene expression by vibrio vulnificus. | strains of vibrio vulnificus incubated in situ in natural estuarine waters during warm months continued to express katg (periplasmic catalase), rpos (stress sigma factor), tufa (elongation factor), wza, and wzb (capsule synthesis). vvha (hemolysin) was differentially expressed between environmental and clinical isolates. these results paralleled our in vitro findings. | 2006 | 16517681 |
| inactivation of vibrio parahaemolyticus and vibrio vulnificus in phosphate-buffered saline and in inoculated whole oysters by high-pressure processing. | inactivation studies for vibrio parahaemolyticus tx-2103 (serotype o3:k6) and vibrio vulnificus mo-624 (clinical isolate) were conducted in phosphate-buffered saline (pbs) and in inoculated oysters under high-pressure processing conditions. v. parahaemolyticus was more resistant than v. vulnificus in pbs at all pressures and times. a 6-log reduction of v. parahaemolyticus and v. vulnificus in pbs at 241 mpa required 11 and 5 min, respectively, which included a 3-min pressure come-up time. a 4.5- ... | 2006 | 16541691 |
| tropical dermatology: bacterial tropical diseases. | bacterial infections are common in tropical parts of the world and can include those species also seen regularly in temperate climates. many tropical bacterial infections, however, are rarely diagnosed in temperate parts of the world and include bartonellosis, tropical ulcer, tropical pyomyositis, granuloma inguinale, lymphogranuloma venereum, yaws, pinta, melioidosis, and glanders. some tropical bacterial diseases, eg, plague and anthrax, are associated with high mortality rates and are of pote ... | 2006 | 16546577 |
| effect of the crp mutation on the utilization of transferrin-bound iron by vibrio vulnificus. | cyclic amp-camp receptor protein (crp) complex plays an essential role in the global regulation of vibrio vulnificus virulence. we found that growth retardation of v. vulnificus caused by mutation of the crp gene encoding crp was exacerbated under iron-limited conditions. accordingly, we investigated the effect of crp mutation on the expression of the vulnibactin-mediated iron-uptake system and the ability of v. vulnificus to utilize transferrin-bound iron, and thus to grow in cirrhotic ascites, ... | 2006 | 16553865 |
| a dish best served hot. | 2006 | 16564770 | |
| coactivation of vibrio vulnificus putap operon by camp receptor protein and putr through cooperative binding to overlapping sites. | the camp receptor protein (crp) positively regulates the expression of vibrio vulnificus putap genes encoding a proline dehydrogenase and a proline permease. in the present study, an open reading frame encoding putr was identified downstream of the putap genes and a mutational analysis revealed that the putr protein was also involved in regulating the putap transcription by activating pput promoter. although crp acts as a primary activator and the influence of putr on pput is mediated by crp, th ... | 2006 | 16573699 |
| occurrence of vibrio parahaemolyticus, v. cholerae, and v. vulnificus in norwegian blue mussels (mytilus edulis). | vibrio parahaemolyticus, v. cholerae, and v. vulnificus were isolated from 10.3%, 1.0%, and 0.1% of 885 blue mussel samples, respectively. four of the samples contained trh(+) v. parahaemolyticus, while no tdh-positive isolates were detected. the v. cholerae isolates were non-o:1/non-o:139 serotypes and were ctxa negative. | 2006 | 16598019 |
| rapidly progressive necrotising fasciitis following a stonefish sting: a report of two cases. | two patients developed rapidly progressive necrotising fasciitis after being stung by a stonefish. both were given a hot-water bath for pain relief. the hot water may have accelerated bacterial growth and the consequent development of necrotising fasciitis. vibrio vulnificus was cultured from one patient. it is recommended that patients should receive high dose of oral and intravenous antibiotic prophylaxis for vibrio prevention. antibiotics should be given before or during, not after, a hot-wat ... | 2006 | 16598091 |
| cloning, sequencing and analysis of the 16s-23s rdna intergenic spacers (igss) of two strains of vibrio vulnificus. | according to the conserved sequences flanking the 3' end of the 16s and the 5' end of the 23s rdnas, pcr primers were designed, and the 16s-23s rdna intergenic spacers (igss) of two strains of vibrio vulnificus were amplified by pcr and cloned into pgem-t vector. different clones were selected to be sequenced and the sequences were analyzed with blast and the software dnastar. analyses of the igs sequences suggested that the strain zsu006 contains five types of polymorphic 16s-23s rdna intergeni ... | 2006 | 16625835 |
| two cases of sucrose-fermenting vibrio vulnificus infection in which 16s rdna sequencing was useful for diagnosis. | vibrio vulnificus is a gram-negative bacterium which is associated with severe infections in humans. we experienced two cases of sucrose-fermenting v. vulnificus infection. the causative agents in both cases were unidentifiable by conventional identification systems because of their unique characteristics, and sequencing of 16s rdna was found to be useful for diagnosis. | 2006 | 16632910 |
| vibrio vulnificus vulnibactin, but not metalloprotease vvpe, is essentially required for iron-uptake from human holotransferrin. | the roles of metalloprotease (vvpe) and catechol-siderophore (vulnibactin) in the uptake of iron from human transferrins by vibrio vulnificus have been determined using different experimental conditions and methods. therefore, in this study, we attempted to elucidate the roles of vvpe and vulnibactin using the same methods and experimental conditions, in an in vitro and a human ex vivo system, and in accordance with the molecular version of koch's postulates. neither vvpe mutation nor in trans v ... | 2006 | 16651718 |
| vibrio vulnificus serovar a: an emerging pathogen in european anguilliculture. | the spread of the emerging pathogen vibrio vulnificus biotype 2 serovar a in danish anguilliculture is reported. serovar a was originally isolated in a spanish eel farm in 2000 and occurred in denmark in the summer of 2004, affecting eels of 5-10 g body weight cultured in fresh water. the danish eels showed clinical signs different from those reported for spanish eels, such as severe haemorrhages in the head and gill region with necrosis of the soft tissues. danish isolates were biochemically an ... | 2006 | 16677318 |
| antibiotic resistance and plasmid profile of environmental isolates of vibrio species from mai po nature reserve, hong kong. | in this study, three environmental isolates of vibrio species were isolated from water and sediment samples of mai po nature reserve, hong kong sar. they were identified to be vibrio cholerae mp-1, vibrio aestuarianus mp-2 and vibrio vulnificus mp-3 by both biochemical test and subsequent full-length 16s rdna sequencing. both disc diffusion susceptibility test and microplate dilution technique were used to investigate the antibiotic resistance profile of these three bacteria. results from both a ... | 2006 | 16680524 |
| atypical manifestation of vibrio vulnificus septicaemia. | vibrio vulnificus is a gram-negative marine bacterium that may cause local wound infection, gastroenteritis, or septicaemia. fatal septicaemia usually presents with fever, shock, and large haemorrhagic bullae on the legs. this report is about a man who had severe v. vulnificus septicaemia but presented with atypical features of leg pain and diffuse purpuric skin lesions. v. vulnificus septicaemia should be suspected if the following are present: septic shock, leg pains associated with diffuse pu ... | 2006 | 16714493 |