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evaluation of the four-hour micro-id technique for direct identification of oxidase-negative, gram-negative rods from blood cultures.a 4-h micro-id technique for direct identification of oxidase-negative gram-negative rods from positive blood cultures was compared to subculture and species identification of single colonies by api 20e and micro-id, using standardized inocula. a total of 127 patients (220 positive cultures) were studied. isolates included 96 escherichia coli, 46 klebsiella pneumoniae, 7 klebsiella oxytoca, 8 enterobacter aerogenes, 17 enterobacter cloacae, 19 serratia marcescens, 2 serratia liquefaciens, 8 prot ...19806999020
repetitions in the nh2-terminal amino acid sequence of beta-ketoadipate enol-lactone hydrolase from pseudomonas putida.muconolactone delta-isomerase (ec 5.3.3.4) and beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24) mediate consecutive reactions in the beta-ketoadipate pathway of bacteria. an earlier investigation (yeh, w.k., davis, g., fletcher, p., and ornston, l.n. (1978) j. biol. chem. 253, 4920-4923) revealed that the respective nh2-terminal amino acid sequences of pseudomonas putida muconolactone isomerase and acinetobacter calcoaceticus beta-ketoadipate enol-lactone hydrolase ii are evolutionarily hom ...19807391022
evolutionary divergence of co-selected beta-ketoadipate enol-lactone hydrolases in acinetobacter calcoaceticus.muconolactone isomerase (ec 5.3.3.4) and beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24) mediate consecutive catabolic steps in bacteria. separately inducible beta-ketoadipate enol-lactone hydrolases i and ii are formed in representatives of acinetobacter calcoaceticus. when subjected to deae-cellulose chromatography, acinetobacter enol-lactone hydrolase i displays heterogeneous behavior which, in whole or in part, appears to be due to modifications of sulfhydryl groups in the protein; the ...19807391023
homologies in the nh2-terminal amino acid sequences of gamma-carboxymuconolactone decarboxylases and muconolactone isomerases.gamma-carboxymuconolactone decarobxylase (ec 4.1.1.44) and muconolactone isomerase (ec 5.3.3.4) mediate chemically analogous reactions in bacteria. the enzymes are inducible, and different metabolites trigger the respective syntheses of the decarboxylases in acinetobacter calcoaceticus and pseudomonas putida. the decarobxylases share similar oligomeric structures in which identical subunits of about 13,300 daltons appear to be self-associated into hexamers. identical residues are found in 18 of ...19807391024
pyrogenic reactions after inadvertent infusion of endotoxin during cardiac catheterizations.in april 1976 the attack rate of chills and fever with or without falls in blood pressure increased in association with cardiac catheterizations. fevers were associated with coronary angiography and right and left heart catheterizations. blood cultures were negative, and reactions did not correlate with amounts of contrast materials infused or with procedures done by a single operator. significant numbers of acinetobacter calcoaceticus (var. anitratus) and a pseudomonas species were cultured fro ...19807396310
symptomatic acinetobacter calcoaceticus peritonitis. "a complication of peritoneal dialysis".acinetobacter calcoaceticus peritonitis was seen during the course of peritoneal dialysis in two patients with end-stage kidney disease within a 3-month period. in one patient, the isolate was a. calcoaceticus varient anitratus (formerly herellea vaginicola) and in the other it was a. calcoaceticus lwoffi (formerly mima polymorpha). both were successfully treated with continuous peritoneal antibiotic lavage.19807440841
naturally occurring plasmids in acinetobacter calcoaceticus: pav2, a plasmid which influences the fertility of the sex factor pav1.acinetobacter calcoaceticus strain ebf65/65 harbours a cryptic plasmid, pav2, which has been shown by electrophoretic separation on agarose gels to have a molecular mass of approximately 13.5 megadaltons (md). transfer of the previously described sex factor pav1 (hinchliffe & vivian, 1980 a,b) from the hospital strainjc17 into strains possessing pav2 occurs only at a low frequency, whereas transfer to similar strains lacking pav2 occurs at a much higher frequency. in ebf65/65, pav1 may be presen ...19807264602
infective endocarditis due to acinetobacter calcoaceticus. 19807205689
influence of growth phase and carbon source on the content of rubredoxin in acinetobacter calcoaceticus.the rubredoxin content of acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, l-malate) and on the growth phase was studied by means of a radioimmunoassay. the method used was specific for rubredoxin and acinetobacter calcoaceticus. the formation of rubredoxin increased with time up to the end of the logarithmic phase when n-alkanes were the sole carbon source. after growth of acinetobacter calcoaceticus on non-hydrocarbon substrates, rubredoxin was not ...19807212930
[epidemiology of acinetobacter calcoaceticus]. 19807243591
an outbreak of acinetobacter infection associated with the use of a ventilator spirometer.although respiratory therapy equipment is a well-known source of nosocomial infection, ventilator spirometers have not been previously implicated. we report 17 acinetobacter calcoaceticus variety anitratus infections traced to contaminated spirometers. isolates from infected patients were recovered from urine, sputum, wounds, and blood. a review of attack rates for acinetobacter was prompted by a dramatic increase in blood culture isolates. prospective surveillance of intensive care environment, ...198010315086
cell surface measurements in hydrocarbon and carbohydrate fermentations.acinetobacter calcoaceticus was grown in 11-liter batch fermentations with hexadecane or sodium citrate as the sole source of carbon. surface and interfacial tension measurements of the microbial broth indicated that surface-active compounds were being produced only during growth on the hydrocarbon substrate. contact angle measurements of an aqueous drop on a smooth lawn of cells in a hexadecane bath indicated a highly hydrophobic surface of the cells in the initial stages of the hydrocarbon fer ...198016345526
nadh and nadph-viologen reductases from acinetobacter calcoaceticus.three pyridine nucleotide-dependent diaphorases have been isolated from acinetobacter calcoaceticus cells and partially characterized. two of them, with molecular weights of 165,000 and 57,000, utilize nadph as electron donor whereas the third one (mw = 57,000) is specific for nadh. oxidized viologen dyes, flavin nucleotides, dichlorophenol indophenol and ferricyanide can act with efficiency as acceptors in the reaction mediated by these diaphorases. the diaphorase activities have been character ...1979223203
[properties of rubredoxin reductase from the alkane-assimilating bacterium acinetobacter calcoaceticus]. 197944771
studies on the oxidation of octane by acinetobacter calcoaceticus and flavobacterium devorans strains. 197994750
serological cross-reactions between acinetobacter calcoaceticus and chlamydiae.a cross-reaction between acinetobacter calcoaceticus and chlamydiae is described. a water-soluble, heat stable, non-dialyzable antigen was extracted from acinetobacter species by boiling. this antigen fixed complement in the presence of homologous hyperimmune sera from rabbits or guinea pigs and in the presence of heterologous human or hyperimmunized animal sera containing chlamydial antibodies. hyperimmune antisera to the extracted antigen, or to suspensions of live acinetobacters, also reacted ...1979521480
[a case of meningitis due to acinetobacter calcoaceticus].meningitis due to acinetobacter calcoaceticus is rare. these rare bacteria were isolated from culture fluid in patient resembling to neisseria meningitis with clinical and laboratory findings. the patient was treated with sensitive antibiotic.1979555785
[uptake of organic acids by acinetobacter calcoaceticus]. 1979543155
[structural features of acinetobacter calcoaceticus k-9 cells assimilating ethanol at different rates of medium dilution]. 1979530115
in vitro activity of three tetracycline antibiotics against acinetobacter calcoaceticus subsp. anitratus.the in vitro activity of three tetracycline antibiotics against 127 strains of acinetobacter calcoaceticus (herella vaginicola) were compared. almost all strains were susceptible to minocycline and doxycycline, whereas most strains were resistant to tetracycline.1979526013
glucose dehydrogenase from acinetobacter calcoaceticus: a 'quinoprotein'. 1979520586
a hand infection from a. calcoaceticus (m. polymorpha).acinetobacter calcoaceticus (mima polymorpha) is not a well known organism, and its identification can be confusing due to its variable morphology and sensitivity pattern. in a joint infection, delay in identification or appropriate treatment can result in added morbidity. an a. calcoaceticus (mima) infection in the hand seems not to have been previously reported. the present case involving a finger joint infection illustrates the pathogenicity of a. calcoaceticus (mima) and problems of treatmen ...1979477074
community-acquired acinetobacter pneumonia.acinetobacter calcoaceticus var anitratus, a nonfermentative grampnegative bacillus, has been infrequently reported as a cause of community-acquired pneumonia. in this paper we describe the course of six recent patients with community-acquired, bacteremic pneumonia due to this organism and review the six previously reported cases. our experience suggests this organism is a more common cause of community-acquired pneumonia than previously thought. acinetobacter pneumonia occurs in older persons w ...1979463916
ventriculitis from acinetobacter calcoaceticus variant anitratus. 1979448387
acinetobacter calcoaceticus: a nosocomial pathogen with an unusual seasonal pattern. 1979448188
aminoglycoside-modifying enzymes among clinical isolates of acinetobacter calcoaceticus subsp. anitratus (herellea vaginicola): explanation for high-level aminoglycoside resistance.acinetobacter calcoaceticus subsp. anitratus (herellea vaginicola) is an important cause of nosocomial infection in our hospital where a. calcoaceticus subsp. anitratus is the most frequently isolated gram-negative species resistant to one or more of the aminoglycoside antibiotics. of 167 strains tested for susceptibility to aminoglycosides, only 6 strains were found that were resistant to >/=128 mug of kanamycin per ml; all others were susceptible to </=32 mug/ml. five of these six strains were ...1979426512
acinetobacter calcoaceticus variety anitratus: an increasing nosocomial problem.acinetobacter calcoaceticus var anitratus is an increasing nosocomial problem in some institutions. duirng a recent 16-month period, we observed 15 patients with blood isolates of this organism. thirteen of these patients acquired the organism from the hospital environment. eleven of these represented real disease and concurrent growth of the organism from another site was predictive of infection (p less than 0.03). a review of laboratory records showed that there was a three-fold increase in no ...1979426000
identification of gram-negative non-fermenters and oxidase-positive fermenters by the oxi/ferm tube.since the recent introduction of the roche oxi/ferm tube to the uk two identification schemes have been developed by the manufacturer for use with the kit. we evaluated the success of these two schemes in identifying 222 predominantly culture collection strains belonging to 45 taxa of non-fermenters and nine taxa of oxidase-positive fermenters. the strains were chosen to represent all the taxa included in the two identification schemes developed by the manufacturer and we have therefore been abl ...1979372251
origins of metabolic diversity: evolutionary divergence by sequence repetition.recurring patterns of primary structure have been observed in enzymes that mediate sequential metabolic reactions in bacteria. the enzymes, muconolactone delta-isomerase [(+)-4-hydroxy-4-carboxymethylisocrotonolactone delta(2)-delta(3)-isomerase, ec 5.3.3.4] and beta-ketoadipate enol-lactone hydrolase [4-carboxymethylbut-3-enolide(1,4)enol-lactone-hydrolase, ec 3.1.1.24], have been coselected in bacterial populations because the isomerase can confer no nutritional advantage in the absence of the ...1979291059
nosocomial respiratory tract infection and colonization with acinetobacter calcoaceticus. epidemiologic characteristics.nosocomial respiratory tract infection with acinetobacter calcoaceticus occurs frequently in many hospitals. an outbreak of respiratory tract infections in an intensive care unit provided an opportunity to study clinical and epidemiologic characteristics of such infections. retrospective studies demonstrated that a. calcoaceticus in sputum was significantly associated with endotracheal intubation (p = 0.03) and continuous positive pressure ventilation (p less than 0.02). after control measures h ...1978362918
[acinetobacter calcoaceticus as the possible cause of bronchopneumonia in rabbits]. 1978352659
evaluation of the oxi/ferm tube system for identification of nonfermentative gram-negative bacilli.the oxi/ferm tube system designed for identification of oxidative-fermentative gram-negative bacteria was evaluated for identification of nonfermentative gram-negative bacilli by comparing it with the conventional system. the nine biochemical reactions used as the initial tests in the oxi/ferm tube system showed a close agreement with the same conventional reactions. however, the system was only 41% accurate to species or biotype for complete identification of 239 isolates which were well distri ...1978353066
evaluation of the api 20e system for identification of nonfermentative gram-negative bacteria.the api 20e system for enterobacteriaceae, recently broadened to include identification of nonfermentative gram-negative bacteria, was evaluated and compared with the conventional method for complete identification of 221 nonfermenters, which were well distributed into 48 species or biotypes and included organisms not listed in the api 20e data base. the results of 16 tests common to both systems were in close agreement. the api 20e system correctly identified 71 (43%) of the 165 organisms inclu ...1978353067
chronic haematuria caused by acinetobacter calcoaceticus in a race horse. 1978676010
phosphatase activity of aerobic and facultative anaerobic bacteria.1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ...1978216188
[kinetic and regulatory properties of malate dehydrogenase from acinetobacter calcoaceticus]. 1978216197
outbreak of a multiply resistant acinetobacter in a surgical intensive care unit: epidemiology and control.during january, 1976 seven patients in an sicu became colonized or infected with acinetobacter calcoaceticus (variation, anitratum) at an attack rate of 12.5 per cent. the organism showed a marked reduction in antimicrobial sensitivity from previous experience. comparison with 34 uninfected control patients indicated that intubation and continuous ventilatory assistance were significantly associated with acquiring the organism (p = 0.0154). acinetobacter was cultured from nurses' hands, ambu ada ...1978248372
microbial and chemical transformations of some 12,13-epoxytrichothec-9,10-enes.resting cells of streptomyces griseus, mucor mucedo, and a growing culture of acinetobacter calcoaceticus when mixed with compounds related to 12,13-epoxytrichothec-9-ene-4beta,15-diacetoxy-3alpha-ol(anguidine) produced a series of derivatives that were either partially hydrolyzed or selectively acylated. these derivatives showed marked differences in activities as assayed by antifungal and tissue culture cytotoxicity tests.1978100053
chronic haematuria caused by acinetobacter calcoaceticus. 1978567878
[oxidation of homologous aliphatic aldehydes by membrane-bound and solubilized aldehyde dehydrogenase from acinetobacter calcoaceticus]. 197832669
genetic analysis of acinetobacter calcoaceticus proline auxotrophs.twenty-six acinetobacter calcoaceticus proline auxotrophs were isolated after ethyl methane sulfonate mutagenesis. studies using the efficient transformation system of this organism indicate that the mutations comprise therr genetically distinct groups.1978618847
equine myositis and septicemia caused by acinetobacter calcoaceticus infection.myositis and septicemia caused by acinetobacter calcoaceticus were diagnosed in a mare. the infection was characterized clinically by ventral swelling and edema, diarrhea, listlessness, and rectal temperature of 39.4 c. the mare was treated symptomatically for 2 days but died on the 3rd day. conditions seen at necropsy were myositis, enteritis, typhlitis, colitis, and hepatitis. lymph nodes were moderately enlarged throughout the body. gross lesions in musculature were edema, scarring, petechiae ...1978621183
[bacteriemia due to acinetobacter calcoaceticus var. anitratus (author's transl)].a serious bacteremia caused by acinetobacter calcoaceticus var. anitratus could be observed. from the clinical point of view the most important symptoms were shivering, high tempeature up to 41 degrees c and clinical signs of acute hepatitis. there were pathological changes in the eeg. certain signs of an outlayer damage of the heart could be seem in the ecg. the pathogenic microorganism isolated from the blood culture media was classified. specific antibodies--agglutinines and precipitines--wer ...1978723766
utilization of oxalacetate by acinetobacter calcoaceticus: evidence for coupling between malic enzyme and malic dehydrogenase.growth of acinetobacter calcoaceticus strain bd413 in malate-mineral medium resulted in the excretion of large quantities of oxalacetate. malate was virtually depleted by the time the cell density reached 60% of its final value; most of the remaining growth took place at the expense of oxalacetate. experiments in which oxalacetate was used as the initial substrate showed that pyruvate was not utilized until most of the oxalacetate disappeared. the generation time for growth on malate or oxalacet ...1978627536
acinetobacter calcoaceticus outbreak associated with peritoneal dialysis.investigation of an outbreak of contamination of dialysis drainage fluid with acinetobacter calcoaceticus var. anitratus identified a previously unrecognized source for dialysis associated infections. over a 4-month period, 25 peritoneal dialysis treatments were administered to 13 hospital patients. of the 25 treatments for which culture results were available, 14 were associated with dialysis drainage fluid cultures positive for a. calcoaceticus. a water bath used to warm bottles of peritoneal ...1978645702
mutants of acinetobacter calcoaceticus ncib 8250 constitutive for the mandelate enzymes.methods for isolating mutants of acinetobacter calcoaceticus ncib 8250 constitutive for the mandelate enzymes were compared. non-inducing substrates were not available. continuous culture gave no stable constitutive mutants. alternate culture in inducing (l-mandelate) and non-inducing (l-glutamate) media led to the isolation of mutants mesoconstitutive for l-mandelate dehydrogenase and phenylgloxylate carboxy-lyase, and hyperinducible for benzaldehyde dehydrogenase i. screening of possible anti- ...1978670930
[cytochrome composition of acinetobacter calcoaceticus].the qualitative and quantitative composition of cytochromes in intact cells of acinetobacter calcoaceticus and in particle fractions obtained from cells following ultrasonic treatment by differential centrifugation were studied using spectrophotometric methods. acinetobacter calcoaceticus contains cytochrome b, cytochrome o and low amounts of cytochrome d. both the absolute content of cytochrome b and o and the relative composition do not essentially vary with the carbon source used (hexadecane, ...1978706933
search for acinetobacter calcoaceticus subsp. anitratus: enrichment of fecal samples.none of 50 healthy individuals and only 2 of 50 hospitalized patients (with acinetobacter anitratus elsewhere) yielded a. anitratus on enrichment from fecal samples.1978701469
chromosome mapping in acinetobacter calcoaceticus.rp4-mediated conjugation was used to map the loci of 23 different mutations on a circular linkage group in acinetobacter calcoaceticus ebf65/65. the resulting genetic map indicated that the chromosomal organization of a. calcoaceticus differed from that of members of the enteric group of bacteria and was similar to pseudomonas in showing an absence of clustering of functionally related genes.1978632801
acinetobacter calcoaceticus: common nosocomial organism, uncommon pathogen. 197810236786
[polarographic determination of oxygen uptake of acinetobacter calcoaceticus during incubation with n-alkanes and their derivatives]. 1977930122
critique: "some aspects of an epidemiological study of nosocomial infections due to acinetobacter calcoaceticus (herellea) featuring bacteriocin typing". 1977585596
some aspects of an epidemiological study of nosocomial infections due to acinetobacter calcoaceticus (herellea) featuring bacteriocin typing. 1977585613
[characteristics of growth and changes in the ultrafine structure of bacteria in the course of continuous cultivation on media containing ethanol].a large number of bacterial strains assimilating chemical ethanol has been isolated using an original technique. active growth of strains belonging to the genera pseudomonas and, particularly, acinetobacter was registered on mineral media containing ethanol. a mathematical model was constructed select a strain of acinetobacter calcoaceticus k-9 during its continuous cultivation on media containing ethanol. the model makes it possible to determine conditions for producing a present amount of the ...1977600096
[uptake of acetate by acinetobacter calcoaceticus].the uptake of acetate by intact nongrowing cells of acinetobacter calcoaceticus was studied in dependence on the c-source (acetate, n-alcanes, yeast extract, succinate, l-malate) and the growth phase. single kinetic parameters of acetate uptake were determined. the best acetate uptake was observed with cells cultivated with acetate as the only c-source. bacteria in the early growth phase were found to transfer acetate twice as fast as cells of the late logarithmic growth phase. the uptake of ace ...1977614747
[oxidation of n-tetradecan-1-14c by cell-free extracts from acinetobacter calcoaceticus]. 1977878503
in vitro synergism between carbenicillin and aminoglycosidic aminocyclitols against acinetobacter calcoaceticus var. anitratus.acinetobacter caleoaceticus var. anitratus is a nonfermentative, gram-negative bacillus that has been demonstrated to cause severe infections, usually in hospitalized patients. since mild to moderate resistance of a. calcoaceticus to one or more aminoglycosidic aminocyclitols has been noted to occur, a study was undertaken to evaluate the activity of combinations of carbenicillin with either kanamycin, tobramycin, or gentamicin against 28 isolates of a. calcoaceticus obtained from clinical sourc ...1977879749
the microbiology of evacuated blood collection tubes.a collaborative study involving 20 clinical laboratories representing diverse geographical regions was organized to examine the microbiology of evacuated blooc collection tubes. overall, 14% of the 1433 tubes examined contained microorganisms representing a variety of bacteria and fungi. of note was the finding that nearly 9% of tubes with yellow closures, which are stated to be sterile by the manufacturers, contained bacteria, including pseudomonas aeruginosa, enterococci, serratia marcescens, ...1977835941
community-acquired acinetobacter calcoaceticus var anitratus pneumonia.two patients had community-acquired acinetobacter calcoaceticus var anitratus pneumonia. both patients were alcoholic and one was cirrhotic. one patient died and the other received two weeks of gentamicin therapy and survived. misinterpretation of the sputum gram stain delayed diagnosis and institution of proper therapy in both cases. in addition to organisms sensitive to penicillins such as neisseria or haemophilus, acinetobacter must be considered in the differential diagnosis of community-acq ...197770540
regulation of synthesis of benzyl alcohol dehydrogenase in acinetobacter calcoaceticus ncib8250.specific activity of benzyl alcohol dehydrogenase in carbon-limited continuous cultures was at a maximum at a specific growth rate of 0.2 h-1, but fell off at lower and higher growth rates. the specific activity in nitrogen-limited cultures was always lower and was inversely proportional to growth rate. there was severe repression of benzyl alcohol dehydrogenase during metabolism of l(+)-mandelate or phenylglyoxylate in batch cultures. synthesis of benzyl alcohol dehydrogenase was followed in ex ...1977201725
solubilization studies and properties of particulate nad-independent malate dehydrogenase of acinetobacter calcoaceticus (n.c.i.b. 8250). 1977196960
[infection by glucose-nonfermenting, gram-negative rods--infection by acinetobacter calcoaceticus]. 1977101606
prosthetic valve endocarditis by opportunistic pathogens.the incidence of endocarditis produced by the so-called "opportunists" as a complication of prosthetic valve surgery is progressively increasing in frequency and gradually transforming the clinical picture habitually associated with this disease. we report six cases of endocarditis produced by opportunistic microorganisms (two cases by candida, and the remaining by serratia, actinobacillus, acinetobacter calcoaceticus, and bacteroides fragilis, and by corynebacterium diphtheriae) in four male an ...1977319774
host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli.the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ...1977329101
[isolation and identification of acinetobacter calcoaceticus from clinical materials and it's clinial value in respiratory infectious diseases (author's transl)]. 1977559145
room humidifiers as the source of acinetobacter infections.twenty-four patients contracted systemic infections with acinetobacter calcoaceticus during a four-month period. unheated room humidifiers at the patients' bedsides were implicated as the source of infection in six. the outbreak was terminated with the removal of the humidifers.1977576319
ecological effects of a deodorant and a plain soap upon human skin bacteria.the effects of a commercial trichlorocarbanilide-containing deodorant soap and a commercial plain soap upon the cutaneous flora of individuals were compared. using a cross-over design, 21 volunteers (10 women and 11 men) washed their forearms at least once a day with one soap for 3 weeks and then switched soaps for another 4 weeks use. by analysis of variance no significant difference in total colony counts was noted among individuals in their use of the two soaps. with the exception of individu ...1977264493
infections with acinetobacter calcoaceticus (herellea vaginicola): clinical and laboratory studies.in a retrospective review of 53 patients, 58 episodes of infection due to acinetobacter calcoaceticus var. anitratus (herellea vaginicola) were studied. although the organism is widely distributed in nature, it is of relatively low virulence since colonization is more frequently noted than infection and since most infections occur in patients subjected to the epidemiologic pressures common to nosocomial, gram-negative bacillary infection: prior antibiotic therapy; instrumentation and manipulatio ...1977846390
[isolation and characterization of cell surface layers of acinetobacter calcoaceticus]. 1977930118
sudden death due to waterhouse-friderichsen syndrome and purulent leptomeningitis caused by acinetobacter calcoaceticus (mima polymorpha).a case of waterhouse-friderichsen syndrome presenting with purulent leptomeningitis causing sudden death is described. the causative agent, acinetobacter calcoaceticus (mina polymorpha), was determined by post-mortem microbiological examination.1977883428
pseudobacteremia: false-positive blood cultures from mist tent contamination.in the seven-month period from july 1975 through january 1976, 11 pediatric patients had acinetobacter calcoaceticus var. anitratus cultured from blood; this organism had not been isolated from pediatric patients in the previous six months. in 10 of 11 patients, only the first of two cultures was positive. all patients recovered uneventfully, although only two were treated with appropriate antibiotics. nine of 11 had been in mist tents at the time of the culture. mist cultured from one tent cont ...1977888818
[induction of nadp+-dependent aldehyde dehydrogenase in acinetobacter calcoaceticus by hydrocarbons]. 1977888459
assimilatory nitrate reductase from acinetobacter calcoaceticus.a soluble nitrate reductase from the bacterium acinetobacter calcoaceticus grown on nitrate has been characterized. the reduction of nitrate to nitrite is mediated by an enzyme of 96000 molecular weight that can use as electron donors either viologen dyes chemically reduced with dithionite or enzymatically reduced with nad(p)h, through specific diaphorases which utilize viologens as electron acceptors. nitrate reductase activity is molybdenumdependent as shown by tungstate antagonistic experimen ...1977849099
[utilization of trimethylammonium-compounds by acinetobacter calcoaceticus (author's transl)].the utilization of carnitine and carnitine derivatives (o-acylcarnitines, carnitine carboxylderivatives) and structure-related trimethylammonium-compounds (betaines and nitrogen-bases) by acinetobacter calcoaceticus was studied by means of the control of growth and the quantitative detection of metabolites. the strain grew only on l-carnitine, l-o-acylcarnitines, and gamma-butyrobetaine as the sole carbon sources. the utilization of these compounds and the growth correlated with the cleavage of ...1977849100
rp4 fertility variants in acinetobacter calcoaceticus. 19761026570
regulation of enzyme synthesis in the tryptophan pathway of acinetobacter calcoaceticus.in acinetobacter calcoaceticus the seven genes coding for the enzymes responsible for tryptophan synthesis map at three chromosomal locations. two three-gene clusters, one (trpgdc) specifying the small subunit of anthranilate synthase, phosphoribosyl transferase, and indoleglycerol phosphate synthase and the other (trpfba) specifying phosphoribosyl anthranilate isomerase and both tryptophan synthase subunits, are not linked to each other or to the trpe gene specifying the large anthranilate synt ...1976931950
the fate of bacteria in frozen red cells.units of blood were intentionally contaiminated with suspensions of either aerobacter aerogenes, escherichia coli, bacillus subtilis, enterobacter cloacae, pseudomonas aeruginosa, sarcina lutea, seratia marcesens, staphylococcus epidermidis, streptococcus faecalis, paracolabacterum aerogenoides (enterobacter hafriae), mima polymorpha or acinetobacter- calcoaceticus. when inoculation was made prior to glycerolization, the subsequent glycerolization, freezing, thawing, and deglycerolization result ...1976936269
purification and properties of l-asparaginase b from acinetobacter calcoaceticus.l-asparaginase (l-asparagine amidohydrolase, ec 3.5.1.1) b from acinetobacter calcoaceticus has been purified by precipitation with streptomycin, chromatography on deae-cellulose and cm-cellulose, gel filtration on agarose and chromatography on phosphocellulose. the molecular weight of the enzyme was found to be 130 000. the enzyme was rather insensitive to ph changes between 7 and 9. the michaelis constant was 3-10(-3) m. hg2+, cu2+, and ni2+ as well as high ionic strength inhibited the activit ...1976938683
[isolation and characterization of rubredoxin from acinetobacter calcoaceticus].acinetobacter calcoaceticus growing on long-chain n-alkanes contains a soluble iron-sulfur protein, which corresponds in its properties to a rubredoxin. it was prepared from the 50000 x g supernatant of ultrasonically treated cells using ion exchange chromatography on deae cellulose and gel filtration on sephadex g-75. the isolated protein is pure electrophoretically, but yields two bands corresponding to molecular weights of 6000 and 12000 respectively. a content of 11 acidic against 6 basic am ...1976970050
regulation of growth of acinetobacter calcoaceticus ncib8250 on benzyl alcohol in batch culture.formation of benzoate and catechol during oxidation of benzyl alcohol by washed suspensions of acinetobacter calcoaceticus ncib8250 confirmed earlier results indicating that this organism metabolizes benzyl alcohol via benzaldehyde, benzoate, and the 3-oxoadipate pathway. there was no evidence for feedback inhibition of benzyl alcohol dehydrogenase or benzaldehyde dehydrogenase ii. examination of growth curves and patterns of substrate utilization, as well as measurement of enzyme activities, sh ...1976993779
susceptibility of nonfermentative gram-negative bacilli to tobramycin.there has been increasing interest in the pathogenic role of nonfermentative gram-negative bacilli in human infections. except for pseudomonas aeruginosa, the susceptibility pattern of these organisms to tobramycin has not been evaluated thoroughly. the activity of tobramycin, as compared with that of gentamicin, was tested by the serial broth dilution technique against 178 isolates of nonfermentative gram-negative bacilli obtained from various sources. p. aeruginosa, pseudomonas stutzeri, acine ...1976823278
in vitro susceptibility of acinetobacter calcoaceticus (herellea vagincola) to tetracycline and minocycline. 1976812659
susceptibility of acinetobacter calcoaceticus var. anitratus (herellea vaginicola) to minocycline.the in vitro activity of minocycline against acinetobacter calcoaceticus var. anitratus (herellea vaginicola) was examined. all strains of a. anitratus were inhibited by minocycline at concentrations of 0.4 mug/ml or less. the strains studied were considerably more susceptible to minocycline than tetracycline. no difference between the susceptibility to minocycline and tetracycline was seen with escherichia coli, klebsiella pneumoniae, enterobacter and proteus sp. a regression line of the log mi ...1976791084
rp4-mediated conjugation in acinetobacter calcoaceticus.the p class r factor rp4 was transferred from escherichia coli k12 to acinetobacter calcoaceticus. rp4 conferred similar levels of antibiotic resistance in a. calcoaceticus to those in the e. coli k12 donor. only slight instability of rp4 in a. calcoaceticus was detected. transfer of rp4 between strains of a. calcoaceticus was by conjugation and was accompanied by transfer of chromosomal genes. possible polarity of marker transfer was observed and linkage between a number of chromosomal markers ...1976778327
preparations and properties of ribonucleic acid polymerase from acinetobacter calcoaceticus.deoxyribonucleic acid (dna)-dependent ribonucleic acid (rna) polymerase (ec 2.7.7.6) from acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the escherichia coli b enzyme. the molecular weights of the two native active enzymes as well as their alpha and beta subunits appeared to be similar. no subunit corresponding to that of sigma from e. coli was found, and furthermore no separation between the beta subunits could be detected by gel ...19761380
purification and various properties of nadp+-dependent alcohol dehydrogenase from acinetobacter calcoaceticus.the constitutive nadp+-dependent alcohol dehydrogenase from acinetobacter calcoaceticus can be accumulated about 50 fold in 3 purification steps. the end-product shows in the analytical polyacrylamide gel electrophoresis only one active enzyme band. the molecular weight of the enzyme was determined to be 235,000 by gel chromatography on sephadex g 200, the smallest subunit shows a molecular weight of 61 000 on sds electrophoresis. the isoelectric point is at 5.84. the km values determined with p ...197614464
in vitro and in vivo activities of minocycline and other antibiotics against acinetobacter (herellea-mima).minocycline was the most active of six antibiotics tested against 65 clinical isolates of acinetobacter calcoaceticus (syn.: herellea, mima) received from six medical centers. in the bauer-kirby disk susceptibility test, all isolates were rated susceptible to minocycline, gentamicin, and polymyxin; 25% were resistant to tetracycline. in agar dilution tests, minocycline was two to four times more potent than gentamicin or polymyxin and eight times more potent than tetracycline. ampicillin and cep ...1976176936
[infective situation in a cow barn contaminated with klebsiella mastitis].the infection situation in a four-row cow-house for 153 animals with a frequent occurrence of klebsiella mastitis was subject to a detailed analysis. the following results were obtained after two collections of blood and udder-quarter milk samples examined by the test-tube agglutination, gel precipitation, and bacteriological diagnosis methods: 1. streptococcus agalactiae was isolated from milk samples 28 and 31 times, pseudomonas aeruginosa 15 and 19 times, staphylococcus arueus 16 and 19 times ...1976828991
catechol 1,2-dioxygenase from acinetobacter calcoaceticus: purification and properties.procedures for the purification of catechol 1,2-dioxygenase from extracts of acinetobacter calcoaceticus strain adp-96 are described. the purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. the enzyme contained 2 g-atoms of iron per mol of protein. the enzyme had a broad substrate specificity and catalyzed the oxidation of catechol, 4-methylcatechol, 3-methylcatechol, and 3-isopropyl catechol. the activity of the enzyme was inhibited by heavy meta ...197658860
emergence of gentamicin-resistant bacteria: experience with tobramycin therapy of infections due to gentamicin-resistant organisms.a computerized system for testing and surveillance of bacterial susceptibility to antibiotics was used in monitoring the emergence of gentamicin-resistant strains of aerobic and facultative gram-negative bacilli at massachusetts general hospital since the release of gentamicin for clinical use in 1971. during the period studied, there was a significant increase in the prevalence of gentamicin-resistant bacteria, particularly among pseudomonas, acinetobacter (herellea), and proteus and, more rece ...1976972284
mandelate dehydrogenase with altered stereospecificity in mutant strains of acinetobacter calcoaceticus n.c.i.b. 8250. 19761001725
constitutivity of the mandelate enzymes in acinetobacter calcoaceticus, n.c.i.b. 8250, and its effect on the synthesis of benzyl alcohol dehydrogenase. 19761022571
protocatechuate 3, 4-dioxygenase from acinetobacter calcoaceticus.protocatechuate 3,4-dioxygenase (pcd) from p-hydroxybenzoate-induced cells of acinetobacter calcoaceticus was purified by heat and protamine sulfate treatment, ammonium sulfate fractionation, deae-cellulose, and sephadex g-200 column chromatography. the enzyme appears to be homogeneous by ultracentrifugation and acrylamide gel electrophoresis. this is the first report of pcd purified from acinetobacter. for comparison, crystalline pseudomonas pcd was also obtained. the enzymes from acinetobacter ...19761252412
conversion of escherichia coli rna polymerase to a template independent enzyme.preparations of rna polymerase (e.c.2.7.7.6) from uninfected escherichia coli, t4 infected escherichia coli, and acinetobacter calcoaceticus when centrifuged in sucrose gradients in the absence of magnesium ions gave rise to five peaks, all of which were able to form polymers from ribonucleoside 5'-triphosphates in the absence of template or primer. all of the peaks obtained from the escherichia coli enzyme appeared to contain the subunit alpha and beta and, in addition, polypeptides which appe ...1976787928
mechanical ventilators as vehicles of infection.twenty-five patients from the intensive care unit who had been subjected to tracheostomy and require mechanical respiration were studied. data are presented concerning the bacteriological controls performed on the tracheal exudates, various parts of the ventilators (engström model 200 and bennett pr-2), and the wright spirometer. the germs found were mainly pseudomonas, enterobacteriaceae germs and bacteria from the acinetobacter group. there was a high incidence of infection in the tracheostomi ...1975811072
metabolism of monofluorobenzoates by acinetobacter calcoaceticus n.c.i.b. 8250. formation of monofluorocatechols.none of the monofluorobenzoates serves as sole source of carbon and energy for growth of acinetobacter calcoaceticus but all can contribute to growth on other substrates. the monofluorobenzoates are oxidised by bacteria pre-induced for benzoate oxidation and can themselves induce the appropriate enzymes. the initial products of oxidation have been separated and identified by gas-liquid chromatography. 2-flurobenzoate is oxidised to catechol, fluoride and 3-fluorocatechol; 3-fluorobenzoate gives ...19751182155
[inhibiton of isocitrate dehydrogenase and isocitrate lyase from acinetobacter calcoaceticus by acids of the citrate and glyoxylate cycle].acinetobacter calcoaceticus contains two forms of nadp+-dependent isocitrate dehydrogenases differing, among others, by their molecular weights and regulatory properties. the regulation of the high-molecular form of isocitrate dehydrogenase and of isocitrate lyase by organic acids, either belonging or related to the citrate and glyoxalate cycle, is investigated. while alpha-ketoglutarate and oxalacetate competitively inhibit the isocitrate dehydrogenase against ds-isocitrate, glyoxylate and pyru ...19751199592
common enterobacterial antigen in yersinia enterocolitica.production of the common enterobacterial antigen (ca) by strains of yersinia enterocolitica (y.e.) serotypes 3 and 9 (winblad), by strains of 5 different y.e. serogroups (wauters) and various other bacteria was examined. antibody against ca was raised by immunization of rabbits with e. coli o 14. extract prepared from s. typhimurium was used for the sensitization of sheep erythrocytes with ca. absorption and haemagglutination inhibition experiments showed that ca could be detected in heat extrac ...19751098394
activity of minocycline against acinetobacter calcoaceticus var. anitratus (syn. herellea vaginicola) and serratia marcescens.the activity of minocycline and tetracycline against 23 isolates of acinetobacter calcoaceticus var. anitratus (syn. herellea vaginicola) and 178 strains of serratia marcescens was determined by disk and microdilution methods. the results indicate that minocycline is highly active against this species of acinetobacter, all but one strain being inhibited by 0.007 mug of the antibiotic per ml. tetracycline was also active, though to a lesser degree, against a. calcoaceticus. of the 178 strains of ...19751099982
correlation between twitching motility and possession of polar fimbriae in acinetobacter calcoaceticus.negatively stained preparations of 16 strains of acinetobacter calcoaceticus were examined in the electron microscope. five of the strains did and 11 of the strains did not exhibit twitching motility. two of the non-twitching strains were substrains of twitching wild-type strains. all twitching strains were found to possess fimbriae with a diameter of approximately 50 a. these fimbriae were shown to be of polar origin except in one strain where, for technical reasons, the origin could not be det ...19751155109
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