Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| localization of the leptospira interrogans metf gene on the cii secondary chromosome. | an open reading frame of 885 nucleotides was identified as the leptospira interrogans metf gene. the deduced amino acid sequence (294 amino acids) showed similarities with escherichia coli methylene tetrahydrofolate reductase (metf or mthfr) (33% identity) and with the n-terminal part of human mthfr (33% identity). the l. interrogans metf gene complements an e. coli metf mutant to prototrophy, suggesting the functionality of the folate branch converging to form methionine. in addition, the l. in ... | 2000 | 11024273 |
| hemolytic and hemoxidative activities in mycoplasma penetrans. | mycoplasma penetrans is a newly isolated mollicute from the urine of patients infected with human immunodeficiency virus that demonstrates the capacity to adhere to and invade human cells. a previous report, based on assays with mouse red blood cells (rbcs), indicated that m. penetrans lacked hemolytic activity. in our studies, we incubated different isolates of m. penetrans with various rbc species and observed hemolytic zones surrounding individual mycoplasma colonies. all m. penetrans strains ... | 2000 | 11035754 |
| the spirochete flaa periplasmic flagellar sheath protein impacts flagellar helicity. | spirochete periplasmic flagella (pfs), including those from brachyspira (serpulina), spirochaeta, treponema, and leptospira spp., have a unique structure. in most spirochete species, the periplasmic flagellar filaments consist of a core of at least three proteins (flab1, flab2, and flab3) and a sheath protein (flaa). each of these proteins is encoded by a separate gene. using brachyspira hyodysenteriae as a model system for analyzing pf function by allelic exchange mutagenesis, we analyzed purif ... | 2000 | 11073915 |
| evaluating genome dynamics: the constraints on rearrangements within bacterial genomes. | inversions and translocations distinguish the genomes of closely related bacterial species, but most of these rearrangements preserve the relationship between the rearranged fragments and the axis of chromosome replication. within species, such rearrangements are found less frequently, except in the case of clinical isolates of human pathogens, where rearrangements are very frequent. | 2000 | 11380986 |
| antibody isotypes in sera of equine fetuses aborted due to leptospira interrogans serovar pomona-type kennewicki infection. | leptospira-specific antibody isotypes in sera of late term equine fetuses aborted due to leptospira interrogans serovar pomona-type kennewicki infection were characterized and compared with those of their dams. igm was the dominant leptospira-specific isotype in both fetuses and mares. however, igga was the isotype in highest concentration in petal sera and strong leptospira-specific igga but no iggb and little or no igg(t) were detected. in contrast, although iggb was quantitatively the dominan ... | 2000 | 11137127 |
| [leptospirosis after a staff outing]. | three male colleagues aged between 34 and 38 years were admitted at the same time to three different rhein-main area hospitals. they presented with a variety of symptoms, including high fever (39.0 to 40.0 degrees c), chills, headache with meningismus or facial paralysis, mild hepatitis and renal involvement. about 18 days before they had been together on a boat rafting tour when the boat capsized when they had fallen into a river in high flood. | 2000 | 11256045 |
| leptospirosis in kuala lumpur and the comparative evaluation of two rapid commercial diagnostic kits against the mat test for the detection of antibodies to leptospira interrogans. | the aim of the study was to look into the epidemiology of serodiagnosed cases of leptospirosis at the university hospital and compare two commercial elisa assays to the microscopic agglutination test (mat). demographic data for all serodiagnosed cases for the years 1991-1997 were collected. from this data, 104 sera (n = 104) were selected as samples for comparative evaluation of the commercial elisas (indx dip-s-ticks and panbio elisa) to the mat test. thirty two (n = 32) negative control sera w ... | 2000 | 11256343 |
| [a molecular epidemiological investigation on leptospira interrogans serovar hebdomadis and australia in china]. | in order to confirm whether cattle serve the source of infection for patients with leptospira interrogans serovar hebdomadis and australia. | 2000 | 11860762 |
| characterization of the leptospira interrogans s10-spc-alpha operon. | a ribosomal protein gene cluster from the spirochaete leptospira interrogans was characterized. this locus is homologous to the escherichia coli s10, spc, and alpha operons. analysis of l. interrogans rna showed that the ribosomal protein genes within this cluster are co-transcribed, thus forming an operon. two transcription initiation sites were mapped by primer extension, upstream of fus, the first gene in this cluster, and sequences from this region provided promoter activity in e. coli. tran ... | 2000 | 10620683 |
| potential role of phospholipases in virulence and fungal pathogenesis. | microbial pathogens use a number of genetic strategies to invade the host and cause infection. these common themes are found throughout microbial systems. secretion of enzymes, such as phospholipase, has been proposed as one of these themes that are used by bacteria, parasites, and pathogenic fungi. the role of extracellular phospholipase as a potential virulence factor in pathogenic fungi, including candida albicans, cryptococcus neoformans, and aspergillus, has gained credence recently. in thi ... | 2000 | 10627494 |
| functional conservation of the polysaccharide biosynthetic protein wbpm and its homologues in pseudomonas aeruginosa and other medically significant bacteria. | wbpm is a highly conserved protein involved in synthesis of the o antigens of pseudomonas aeruginosa. homologues of this protein have been identified in a large number of bacteria, and they can be divided into two subfamilies: subfamily 1, including wbpm, contains large proteins ( approximately 600 amino acids), while subfamily 2, typified by hp0840 (flaa1) of helicobacter pylori, contains smaller proteins ( approximately 350 amino acids) homologous to the c termini of proteins in subfamily 1. a ... | 2000 | 10639466 |
| monoclonal antibodies suitable for incorporation into a competitive enzyme-linked immunosorbent assay (elisa) for detection of specific antibodies to leptospira interrogans serovar pomona. | monoclonal antibodies (mab) were produced by fusing sp2/0-ag14 myeloma cells with spleen cells from balb/c and nd4 mice that were immunized with killed leptospira interrogans serovar pomona whole cells. thirty hybridomas which produced antibodies (of the igg1, igg2a, igg2b, or igg3 isotype) that bound to epitopes on the serovar pomona whole cell antigen were identified by an indirect enzyme-linked immunosorbent assay (elisa). twenty-eight of these 30 mabs cross-reacted in the indirect elisa with ... | 2000 | 10665542 |
| development of an indirect enzyme-linked immunosorbent assay for the detection of leptospiral antibodies in dogs. | serology plays an important role in the diagnosis of leptospirosis. few laboratories have the resources, expertise, or facilities to perform the microscopic agglutination test (mat). thus, there is a need for a rapid and simple serological test that could be used in any diagnostic laboratory. in this study, a genus-specific, heat-stable antigenic preparation from leptospira interrogans serovar pomona was used in an enzyme-linked immunosorbent assay (elisa) for the detection of leptospiral antibo ... | 2000 | 10680654 |
| a clonal subpopulation of leptospira interrogans sensu stricto is the major cause of leptospirosis outbreaks in brazil. | leptospira is a highly diverse genus comprising many species and serogroups in brazil as well as all over the world. however, a study by arbitrarily primed pcr of 44 leptospiral strains isolated from humans during three different outbreaks in brazilian urban centers reveals that 43 of 44 isolates exhibit very similar fingerprints. analysis of these isolates indicates that they belong to a clonal subpopulation of leptospira interrogans sensu stricto. | 2000 | 10618140 |
| cloning, sequencing, and transcriptional analysis of the dnak heat shock operon of listeria monocytogenes. | the complete dnak operon of listeria monocytogenes was isolated by chromosome walking using the previously cloned dnak gene as a probe. molecular analysis of the locus identified 6 genes in the order hrca, grpe, dnak, dnaj, orf35, and orf29. primer extension analysis revealed 3 transcription start sites-s1, s2, and s3-upstream of the hrca, grpe, and dnaj, respectively. the transcription from s1 was heat inducible. analysis of the sequences revealed the consensus promoter sequences of gram-positi ... | 2000 | 10701836 |
| microbial relatives of the seed storage proteins of higher plants: conservation of structure and diversification of function during evolution of the cupin superfamily. | this review summarizes the recent discovery of the cupin superfamily (from the latin term "cupa," a small barrel) of functionally diverse proteins that initially were limited to several higher plant proteins such as seed storage proteins, germin (an oxalate oxidase), germin-like proteins, and auxin-binding protein. knowledge of the three-dimensional structure of two vicilins, seed proteins with a characteristic beta-barrel core, led to the identification of a small number of conserved residues a ... | 2000 | 10704478 |
| [report on a leptospirosis outbreak in the city of santa fe, argentina, march-april 1998]. | in march-april 1998 in a neighborhood in the city of santa fe, argentina, there was an outbreak of an acute disease characterized by fever, headaches, and intense myalgias. this article presents the studies surrounding this outbreak and the attempts to identify the source and the mode of transmission. the epidemiological, serological, and clinical findings indicated that the causative agent was leptospira interrogans. as a screening test, macroscopic agglutination with heat-resistant antigen was ... | 2000 | 10715972 |
| the leptospiral major outer membrane protein lipl32 is a lipoprotein expressed during mammalian infection. | we report the cloning of the gene encoding the 32-kda lipoprotein, designated lipl32, the most prominent protein in the leptospiral protein profile. we obtained the n-terminal amino acid sequence of a staphylococcal v8 proteolytic-digest fragment to design an oligonucleotide probe. a lambda-zap ii library containing ecori fragments of leptospira kirschneri dna was screened, and a 5.0-kb dna fragment which contained the entire structural lipl32 gene was identified. several lines of evidence indic ... | 2000 | 10722630 |
| identification of a 36-kda fibronectin-binding protein expressed by a virulent variant of leptospira interrogans serovar icterohaemorrhagiae. | we investigated the ability of a virulent strain of leptospira interrogans serovar icterohaemorrhagiae, its isogenic avirulent variant and a saprophytic strain to bind fibronectin using alkaline phosphatase-labelled fibronectin. a single 36-kda fibronectin-binding protein was expressed only by the virulent strain and was located in the outer sheath according to proteinase k treatment results. the interaction of this protein with fibronectin was specific and the region of fibronectin bound to thi ... | 2000 | 10731601 |
| analysis of leptospira isolates from mainland portugal and the azores islands. | from 228 recent leptospira isolates from mainland portugal and azorean wild mammals, 149 were characterized at the serovar level by monoclonal antibodies (mabs), a quick serological method in epidemiological studies. in order to compare this antigenic information with that from new genetic techniques, a sample of isolates was analyzed through pulsed-field agarose gel electrophoresis (pfge) (n=71), mapped restriction site polymorphisms (mrsps) in pcr-amplified rrna genes (n=45, including 13 sapro ... | 2000 | 10754245 |
| genome plasticity among related ++lactococcus strains: identification of genetic events associated with macrorestriction polymorphisms. | the genomic diversity of nine strains of the lactococcus lactis subsp. cremoris (ncdo712, ncdo505, ncdo2031, ncdo763, mms36, c2, lm0230, lm2301, and mg1363) was studied by macrorestriction enzyme analysis using pulsed-field gel electrophoresis. these strains were considered adequate for the investigation of genomic plasticity because they have been described as belonging to the same genetic lineage. comparison of apai and smai genome fingerprints of each strain revealed the presence of several m ... | 2000 | 10762249 |
| detection and differentiation of leptospira spp. serovars in bovine semen by polymerase chain reaction and restriction fragment length polymorphism. | in view of the importance of venereal transmission of bovine leptospirosis, the objective of the present study was to apply the polymerase chain reaction (pcr) to 26 serovars of leptospira interrogans, l. borgpetersenii, l. santarosai, l. noguchii and l. biflexa, to determine the detection threshold in semen samples and to evaluate the possibility of differentiation among serovars using 19 restriction endonucleases. the results showed that all serovars were amplified and the detection threshold ... | 2000 | 10781725 |
| serosurvey for selected infectious disease agents in free-ranging black and white rhinoceros in africa. | two hundred and eighty one serum samples collected from free-ranging black (diceros bicornis) and white (ceratotherium simum) rhinoceros, in the republic of south africa (rsa), namibia, and kenya from 1987-97, were examined for antibody to 16 different infectious agents. positive antibody titers were detected against akabane (59.8%), bluetongue (55%), african horse sickness (27.9%), epizootic haemorrhagic disease of deer (19.4%), parainfluenza type 3 (25.3%), bovine herpes virus 1 (3.1%), equine ... | 2000 | 10813614 |
| cloning and characterization of the mammalian brain-specific, mg2+-dependent neutral sphingomyelinase. | the enzymatic breakdown of sphingomyelin by sphingomyelinases is considered the major source of the second messenger ceramide. studies on the contribution of the various described acidic and neutral sphingomyelinases to the signaling pool of ceramide have been hampered by the lack of molecular data on the neutral sphingomyelinases (nsmases). we recently identified a mammalian nsmase, an integral membrane protein with remote similarity to bacterial sphingomyelinases. however, its ubiquitous expre ... | 2000 | 10823942 |
| rpob gene analysis as a novel strategy for identification of spirochetes from the genera borrelia, treponema, and leptospira. | spirochetes are emerging pathogens for which culture and identification are partly unresolved. in fact, 16s rrna-based sequencing is by far the most widely used pcr methodology that is able to detect such uncultivable pathogens. however, this assay actually has some limitations linked to potential problems of contamination, which hampers diagnosis. to circumvent this, we have devised a simple pcr strategy involving targeting of the gene encoding the rna polymerase beta subunit (rpob), a highly c ... | 2000 | 10834976 |
| three case studies involving leptospira interrogans serovar pomona infection in mixed farming units. | three case studies involving leptospira interrogans serovar pomona outbreaks within mixed farming systems in south africa are described. on 2 farms, pigs constituted the main enterprise with cattle and sheep of secondary importance. on each of these 2 farms, abortion due to l. pomona in sows was confirmed by culture, and antibody titres to pomona were detected in cattle, sheep, horses and dogs. on the 3rd farm, a piggery was of secondary importance to cattle farming. abortion and death in cows o ... | 1999 | 10855820 |
| expression and immunologic characterization of recombinant heat shock protein 58 of leptospira species: a major target antigen of the humoral immune response. | a clone of leptospira interrogans serovar lai that was isolated by immunoscreening of a genomic lambda library with sera from convalescent patients with leptospirosis directed expression of a unique 62-kda protein in escherichia coli. when examined by sds-page, the protein comigrated with an immunodominant protein present in leptospiral cell lysate. determination of the nucleotide sequence of the 2.7-kb insert dna identified two genes homologous to the hsp58 and hsp10 of l. interrogans serovar c ... | 1999 | 10619602 |
| arbitrarily primed pcr to type vibrio spp. pathogenic for shrimp. | a molecular typing study on vibrio strains implicated in shrimp disease outbreaks in new caledonia and japan was conducted by using ap-pcr (arbitrarily primed pcr). it allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. clusters identified within the species vibrio penaeicida were related to their area of origin, allowing discrimination between japanese and new caledonian isolates, as well as between th ... | 1999 | 10049875 |
| antibodies against leptospira interrogans in california sea lion pups from gulf of california. | one hundred and twenty-five serum samples from california sea lion (zalophus californianus californianus) pups, and one from an adult female from eight reproductive rookeries located in seven islands in the gulf of california (mexico), were collected during the 1994-96 reproductive seasons. these were tested for antibodies to 19 serovars of leptospira interrogans using a microscopic agglutination test (mat). forty-one samples (32%) had antibody levels from 1:20 to 1:320 to one or more serovars. ... | 1999 | 10073358 |
| serosurvey for antibodies against brucella abortus and leptospira interrogans in pampas deer from brazil. | a survey for antibodies against brucella abortus, and leptospira interrogans was conducted on 17 pampas deer (ozotocerus bezoarticus) from pantanal matogrossense (state of mato grosso do sul, brazil) and on 24 pampas deer from parque nacional de emas (state of goiás, brazil). antibodies against b. abortus were detected by plate agglutination, rose bengal, and complement fixation tests; antibodies against leptospira interrogans were detected by the microscopic agglutination test. all sera were ne ... | 1999 | 10073359 |
| three cases of canine leptospirosis in quebec. | three dogs from different locations with acute renal failure were hospitalized in autumn 1996 and 1997. leptospira interrogans serovar pomona was detected by the microscopic agglutination test. all dogs recovered after antibiotic treatment. the importance of the development of vaccines adapted to emerging serovars in dogs should be addressed. | 1999 | 10086220 |
| risk factors associated with the seroprevalence of leptospirosis among students at the veterinary school of zaragoza university. | the prevalence and risk factors associated with leptospirosis were studied in veterinary students in zaragoza. sera were collected at the beginning and end of the academic year 1994 to 1995 and were tested by elisa against a pool of leptospira interrogans serovars bratislava, canicola, grippotyphosa, hardjo, icterohaemorrhagiae and pomona antigens. at the beginning of the study the prevalence was 8.14 per cent and at the end it was 11.4 per cent. the incidence of the disease during the study was ... | 1999 | 10204224 |
| investigation on process parameters involved in preparation of poly-dl-lactide-poly(ethylene glycol) microspheres containing leptospira interrogans antigens. | block copolymer, poly-dl-lactide-poly(ethylene glycol) (pela) with 11.5% of poly(ethylene glycol) (peg) content was prepared by bulk ring-opening polymerization using stannous chloride as initiator. pela microspheres with entrapped leptospira interrogans antigens, outer membrane protein (omp) were elaborated by solvent extraction method based on the formation of multiple w/o/w emulsion, and the resulting microspheres were characterized with respect to particle size, omp entrapment and morphology ... | 1999 | 10205644 |
| serologic survey for selected infectious disease agents in raccoons from illinois. | the determination of serologic titers to infectious organisms is a valuable tool for quantitating exposure to disease organisms. raccoons (procyon lotor) were live-trapped from september 1989 to october 1993 and samples collected from two distinct locations in west-central illinois (usa); a state recreational facility (park) and privately owned farming property (farm). sera were submitted for testing leptospira interrogans (serovars bratislava, canicola, grippotyphosa, hardjo, icterohemmorhagiae ... | 1999 | 10231761 |
| further determination of dna relatedness between serogroups and serovars in the family leptospiraceae with a proposal for leptospira alexanderi sp. nov. and four new leptospira genomospecies. | dna relatedness was determined among 303 strains of leptospira and leptonema. included in the analysis were reference strains from 228 well-characterized and recognized serovars. the study included 268 serovars from 29 named and one or more unnamed serogroups. the strains clustered into 17 dna hybridization groups, representing 12 previously described species (292 strains) and five new genomospecies (11 strains). the largest groups included leptospira interrogans (91 strains from 82 serovars), l ... | 1999 | 10319510 |
| access of antibody or trypsin to an integral outer membrane protein (p66) of borrelia burgdorferi is hindered by osp lipoproteins. | the outer membrane of borrelia burgdorferi, the lyme disease agent, contains lipoproteins anchored by their lipid moieties and integral proteins with membrane-spanning regions. we used the techniques of in situ proteolysis, immunofluorescence, in vitro growth inhibition, and cross-linking with formaldehyde to characterize topological relationships between p66, an integral membrane protein, and selected osp lipoproteins of b. burgdorferi. protease treatment of intact spirochetes cleaved p66 and o ... | 1999 | 10338494 |
| igg western blot as a confirmatory test in early syphilis. | sensitivity and specificity of igg detection by western blotting performed with a lysate of treponema pallidum whole cells were compared with those of the most common assays used in the laboratory diagnosis of syphilis, i.e. fluorescent treponemal antibody absorption test (fta-abs) and treponemal haemagglutination assay (tpha). thirty-five serum samples obtained from twenty-one patients with a clinical diagnosis of early syphilis, based on the presence of typical chancre or skin or mucous membra ... | 1999 | 10360313 |
| validity of interpretation criteria for standardized western blots (immunoblots) for serodiagnosis of lyme borreliosis based on sera collected throughout europe. | western blotting (wb; immunoblotting) is a widely used tool for the serodiagnosis of lyme borreliosis (lb), but so far, no generally accepted criteria for performance and interpretation have been established in europe. the current study was preceeded by a detailed analysis of wb with whole-cell lysates of three species of borrelia burgdorferi sensu lato (u. hauser, g. lehnert, r. lobentanzer, and b. wilske, j. clin. microbiol. 35:1433-1444, 1997). in that study, interpretation criteria for a pos ... | 1999 | 10364592 |
| use of recombinant flagellin protein as a tracer antigen in a fluorescence polarization assay for diagnosis of leptospirosis. | the objective of the present study was to investigate the usefulness of a recombinant flagellar protein, flab, of leptospira interrogans serovar pomona in the serodiagnosis of leptospirosis by the fluorescence polarization assay (fpa). the recombinant protein flab was purified to homogeneity by a combination of nickel-nitriloacetic acid agarose chromatography, electrophoresis, and electroelution. purified flab was labeled with fluorescein isothiocyanate (fitc). western blotting was performed by ... | 1999 | 10391870 |
| the smcl gene of listeria ivanovii encodes a sphingomyelinase c that mediates bacterial escape from the phagocytic vacuole. | the ruminant pathogen listeria ivanovii differs from listeria monocytogenes in that it causes strong, bizonal haemolysis and a characteristic shovel-shaped co-operative haemolytic ('camp-like') reaction with rhodococcus equi. we cloned the gene responsible for the differential haemolytic properties of l. ivanovii, smcl. it encodes a sphingomyelinase c (smase) highly similar (> 50% identity) to the smases from staphylococcus aureus (beta-toxin), bacillus cereus and leptospira interrogans. smcl wa ... | 1999 | 10417642 |
| incidence of leptospiral abortion in brazilian dairy cattle. | we examined dams' paired serum samples and foetal kidneys (histopathologically and attempting leptospiral cultures) from 120 brazilian holstein abortions from 10 herds near sao paulo, brazil, in 1996. leptospiras was isolated from 15 foetuses. the leptospira interrogans serovar hardjo was obtained from four, pomona from three and wolffi from eight foetuses. ten of these 15 foetuses showed a four-fold rise in titres between the day of abortion and the second samples taken 15 days later. fifty-sev ... | 1999 | 10423780 |
| dna relatedness of leptospira strains isolated from beef cattle in zimbabwe. | the dna relatedness of 17 leptospira strains isolated from beef cattle in zimbabwe was determined using the hydroxyapatite method. similarly to previously speciated african strains, all zimbabwe isolates belonged to either leptospira borgpetersenii or leptospira kirschneri. all serovars within serogroups pyrogenes (kwale, mombe and a strain closely related to serovar nigeria), hebdomadis (marondera and mhou), tarassovi (ngavi) and sejroe (balcanica and hardjo) were l. borgpetersenii. l. kirschne ... | 1999 | 10425768 |
| infertility and abortion among first-lactation dairy cows seropositive or seronegative for leptospira interrogans serovar hardjo. | to estimate the extent to which exposure to leptospira hardjo before or at the time of first parturition was associated with infertility and abortion during the first lactation among dairy cows that had not been vaccinated for > or = 12 months. | 1999 | 10461637 |
| comparative analysis of the lps biosynthetic loci of the genetic subtypes of serovar hardjo: leptospira interrogans subtype hardjoprajitno and leptospira borgpetersenii subtype hardjobovis. | although leptospira borgpetersenii subtype hardjobovis and l. interrogans subtype hardjoprajitno belong to different species, they are serologically indistinguishable and are therefore classified as serovar hardjo. since lps is the major antigen involved in serological classification, this implies that the lps of these subtypes is identical. comparison of the lps biosynthetic loci (rfb) of the subtypes revealed remarkable similarity, with 32 and 31 origins of replication (orfs) in the hardjopraj ... | 1999 | 10474199 |
| health evaluation of a pronghorn antelope population in oregon. | during 1996 and 1997, the u.s. fish and wildlife service conducted a study to determine the cause(s) of population decline and low survival of pronghorn antelope (antilocapra americana) fawns on hart mountain national antelope refuge (hmnar) located in southeastern oregon (usa). as part of that study, blood, fecal, and tissue samples from 104 neonatal fawns, 40 adult does, and nine adult male pronghorns were collected to conduct a health evaluation of the population. physiological parameters rel ... | 1999 | 10479084 |
| urban epidemic of severe leptospirosis in brazil. salvador leptospirosis study group. | leptospirosis has, traditionally, been considered a sporadic rural disease. we describe a large urban outbreak of leptospirosis. | 1999 | 10485724 |
| phylogenetic analysis of leptospira strains of pathogenic serovars using 23s rdna gene sequences. | the 23s ribosomal dnas were amplified from 11 strains of leptospira interrogans sensu lato by polymerase chain reaction (pcr) and sequenced. the pcr products of about 290-bp dna fragments indicated more than 97% sequence similarity to each other. the phylogenetic tree based on the 23s ribosomal dnas obtained in this study revealed that 11 strains of l. interrogans examined composed a cluster distinct to that of l. weilii and l. borgpetersenii, confirming that these strains were similar to strain ... | 1999 | 10522384 |
| repetitive sequence of leptospira interrogans serovar icterohaemorrhagiae strain ictero no. 1: a sensitive probe for demonstration of leptospira interrogans strains. | a 4.8-kilobase (kb) repetitive sequence element generated with kpni digestion was cloned from the leptospira interrogans serovar icterohaemorrhagiae strain ictero no. 1. the sequence, repeated in tandem, was located on the 280-kb fragment between the fsei and asci sites on the chromosome by hybridization using the 4.8-kb fragment as a probe. we cloned the fragment containing the element for the ictero no. 1 strain in a lambda embl3 bacteriophage dna, and one out of 5 clones was sequenced. within ... | 1999 | 10529108 |
| expression in escherichia coli of flab, the gene coding for a periplasmic flagellin of leptospira interrogans serovar pomona. | a periplasmic flagellin gene, flab, of leptospira interrogans serovar pomona (strain pomona) was expressed in escherichia coli for the production and antigenic characterisation of the protein. the flab structural gene, which was previously cloned into puc118, was derived by pcr from the recombinant plasmid and used to generate an expression construct with the trc promoter-driven pproex ht system. under the conditions employed, the flab was expressed as inclusion bodies formed within e. coli, yie ... | 1999 | 10535640 |
| a protein-based phylogenetic tree for gram-positive bacteria derived from hrca, a unique heat-shock regulatory gene. | the dnak operon from bacillus subtilis and other gram-positive bacteria with low g + c dna content contains additional heat-shock genes, including hrca. the hrca gene encodes a transcription factor that negatively regulates heat-shock genes and is uniformly present in all gram-positive bacteria studied to date. an hrca homologue is also present in synechocystis species, leptospira interrogans, chlamydia trachomatis, caulobacter crescentus and methanococcus jannaschii, organisms that diverged ear ... | 1999 | 10555317 |
| direct and rapid detection by pcr of erysipelothrix sp. dnas prepared from bacterial strains and animal tissues. | a pcr method for rapid screening of erysipelothrix spp. in the slaughterhouse was carried out by using four species-specific sets of oligonucleotide primers after initial amplification with the primer set mo101-mo102, which amplifies the 16s rrna sequences of all four erysipelothrix species. the dna sequences coding for the rrna gene cluster, including 16s rrna, 23s rrna, and the noncoding region downstream of 5s rrna, were determined in order to design primers for the species-specific pcr detec ... | 1999 | 10565937 |
| leptospiral outer membrane proteins ompl1 and lipl41 exhibit synergistic immunoprotection. | new vaccine strategies are needed for prevention of leptospirosis, a widespread human and veterinary disease caused by invasive spirochetes belonging to the genus leptospira. we have examined the immunoprotective capacity of the leptospiral porin ompl1 and the leptospiral outer membrane lipoprotein lipl41 in the golden syrian hamster model of leptospirosis. specialized expression plasmids were developed to facilitate expression of leptospiral proteins in escherichia coli as the membrane-associat ... | 1999 | 10569777 |
| serological survey for antibodies against selected infectious agents among fallow deer (dama dama) in central italy. | sera were collected from 224 fallow deer (dama dama) in reserves in central italy. samples were tested for antibodies against chlamydia spp., brucella spp. and coxiella spp. with the complement fixation (cf) test. indirect immunofluorescence was used to test for antibodies against borrelia spp. and agglutination tests were conducted for leptospira interrogans antibodies. enzyme-linked immunosorbent assay (elisa) tests were used to detect antibodies against bovine herpesvirus-1 (bhv-1) and bovine ... | 1999 | 10574071 |
| a dna fragment of leptospira interrogans encodes a protein which shares epitopes with equine cornea. | horses infected with leptospira interrogans present several clinical disorders, one of them being recurrent uveitis. an antigenic relationship between this bacterium and equine cornea has been described in previous studies. with the aim to make progress on defining the molecular basis and pathogenesis of equine recurrent uveitis, here we describe the cloning of one dna fragment from a leptospira interrogans serovar pomona genomic lambda gt11 library. although there are references of transcriptio ... | 1999 | 10587299 |
| [an old disease with a new face: canine leptospirosis does not lose its relevance]. | the clinical features of the disease are presented based on retrospective analysis of the records of eleven dogs diagnosed with leptospirosis using clinical signs and results of the microagglutination test (mat) between 1991 and 1996. additionally, leptospira titres were determined in 30 healthy dogs and 20 hospitalised dogs without clinical or laboratory evidence of leptospirosis. a positive titre for l. grippotyphosa, l. pomona, l. bratislava, l. australis, l. icterohaemorrhagiae and/or l. can ... | 1999 | 10596270 |
| differential and independent roles of a sigma(32) homolog (rpoh) and an hrca repressor in the heat shock response of agrobacterium tumefaciens. | the heat shock response in alpha proteobacteria is unique in that a combination of two regulators is involved: a positive regulator, rpoh (sigma(32) homolog), found in the alpha, beta, and gamma proteobacteria, and a negative regulator, hrca, widely distributed in eubacteria but not in the gamma proteobacteria. to assess the differential roles of the two regulators in these bacteria, we cloned the hrca-grpe operon of agrobacterium tumefaciens, analyzed its transcription, and constructed deletion ... | 1999 | 10601208 |
| expression and distribution of leptospiral outer membrane components during renal infection of hamsters. | the outer membrane of pathogenic leptospira species grown in culture media contains lipopolysaccharide (lps), a porin (ompl1), and several lipoproteins, including lipl36 and lipl41. the purpose of this study was to characterize the expression and distribution of these outer membrane antigens during renal infection. hamsters were challenged with host-derived leptospira kirschneri to generate sera which contained antibodies to antigens expressed in vivo. immunoblotting performed with sera from ani ... | 1999 | 9916100 |
| production and characterization of monoclonal antibodies specific for leptospira borgpetersenii serovar hardjo type hardjobovis and leptospira interrogans serovar hardjo type hardjoprajitno. | murine monoclonal antibodies were produced by immunizing balb/c mice with a killed whole-cell antigen prepared from leptospira borgpetersenii serovar hardjo type hardjobovis. six of these antibodies recognized epitopes on the homologous antigen and on whole-cell antigen prepared from leptospira interrogans serovar hardjo type hardjoprajitno. these antibodies did not cross-react with whole-cell antigens prepared from l. borgpetersenii serovar sejroe, 10 other pathogenic leptospira serovars, or th ... | 1999 | 9918336 |
| leptospirosis in portugal: epidemiology from 1991 to 1997. | the term leptospirosis describes a group of diseases caused by members of the order spirochaetales, mainly the species leptospira interrogans. infection can be asymptomatic but disease of differing severity and clinical features (often suggestive of menin | 1999 | 12631907 |
| retinal immunopathology in horses with uveitis. | equine uveitis is a spontaneous disorder of horses that can serve as a model for the study of human uveitis. although the initial presentation is that of an anterior uveitis, retinal involvement has been noted in some cases. we report here the immunohistopathology of retinas from horses with uveitis. | 1998 | 9924920 |
| infection rate of leptospira interrogans in the field rodent, apodemus agrarius, in korea. | leptospirosis has significantly decreased in korea since 1988, following the leptospiral vaccination programme initiated in 1988. whether this wholly explains the decreased incidence is uncertain. as an initial step to answer this question, infection rates of leptospira interrogans in field rodents, apodemis agrarius, were examined and compared with previous data. two hundred and twenty-two a. agrarius were captured during october-december 1996. spirochaetes were isolated from 22 (9.9%) and lept ... | 1998 | 10030719 |
| [standardization of the lethal dose 50% of leptospira interrogans strains used in the control of the leptospirosis cuban vaccine for human use]. | the ld50 of strains of l. interrogans used in the potency assay of the cuban trivalent leptospiral vaccine intended for human use was standardized. the control of the leptospires content of the cultures by means of the adjusting dilution (10 to 12 leptospires per field) was introduced. that was the key of the standardization of the assay, since it guaranteed the reproducibility of the estimate of the ld50 value for each serogroup: 10(5.98) for canicola, 10(5.60) for icterohaemorrhagiae and 10(6. ... | 1998 | 9842263 |
| in vivo apoptosis of hepatocytes in guinea pigs infected with leptospira interrogans serovar icterohaemorrhagiae. | to investigate the contribution of the previously demonstrated in vitro apoptosis to the pathogenesis of leptospirosis, guinea pigs were infected with leptospira interrogans serovar icterohaemorrhagiae strain verdun and sequentially killed to collect target organs involved in the natural history of the disease (liver, kidneys, lungs, spleen and heart). the combination of histopathological procedures and a specific tunel assay showed a significant leptospira-induced programmed cell death of hepat ... | 1998 | 9851039 |
| apoptosis of lymphocytes in mice administered lipopolysaccharide from leptospira interrogans. | we report that administration of leptospiral lipopolysaccharide (lps) in mice results in massive surface marker changes in the lymphocytes of the spleen. it appears that many of these changes relate to the large number of cells undergoing apoptosis. it is also shown that tumor necrosis factor-alpha (tnf-alpha) induces similar effects and is produced in large quantities after injection of leptospiral lps. it is likely that cytokines such as tnf-alpha are involved in apoptosis. | 1998 | 9852768 |
| [demonstration of intraocular leptospira in 4 horses suffering from equine recurrent uveitis (eru)]. | vitreous samples from 43 horses which underwent vitrectomy because of equine recurrent uveitis (eru) were cultured for leptospires. out of 4 vitreous samples (4/43 = 9%), leptospires could be isolated. in 3 cases, serovar grippotyphosa, and in one case, a serovar out of the serogroup australis were identified. so for the first time, in several horses with eru in vivo cultures of vitreous material were positive for leptospires. a strong evidence of association between leptospiral infection and uv ... | 1998 | 9880935 |
| evaluation of the indirect hemagglutination assay for diagnosis of acute leptospirosis. | serology plays an important role in the diagnosis of leptospirosis. few laboratories have the resources and expertise to perform the microscopic agglutination test. there is a need for rapid and simple serological tests which facilitate the early diagnosis of leptospirosis, while antibiotic therapy may be most effective. a commercially available indirect hemagglutination assay (iha; mrl diagnostics, cypress, calif.) was evaluated with multiple serum specimens from 107 patients being investigated ... | 1998 | 9431911 |
| direct sulfhydrylation for methionine biosynthesis in leptospira meyeri. | a gene library of the leptospira meyeri serovar semaranga strain veldrat s.173 dna has been constructed in a mobilizable cosmid with inserts of up to 40 kb. it was demonstrated that a leptospira dna fragment carrying mety complemented escherichia coli strains carrying mutations in metb. the latter gene encodes cystathionine gamma-synthase, an enzyme which catalyzes the second step of the methionine biosynthetic pathway. the mety gene is 1,304 bp long and encodes a 443-amino-acid protein with a m ... | 1998 | 9440513 |
| coiling phagocytosis discriminates between different spirochetes and is enhanced by phorbol myristate acetate and granulocyte-macrophage colony-stimulating factor. | the mechanisms involved in coiling phagocytosis are not yet known, and it is not even clear whether this phenomenon is either an incidental event or a specific response. therefore, the phagocytic uptake of borrelia burgdorferi and other spirochetes by human monocytes in vitro was used to investigate the involvement of both sides--microbes and phagocytes--in coiling phagocytosis. as seen with electron microscopy, morphologically similar borrelia, leptospira and treponema strains induced markedly ... | 1998 | 9453619 |
| leptospira interrogans antibodies in feral pigs from new south wales. | the sera of 195 hunter-killed feral pigs (sus scrofa), collected in new south wales (australia) from april to november 1995, were screened against a reference panel of 14 leptospira interrogans serovars using a microscopic agglutination test (mat). the panel represented those serovars previously isolated from wild and domestic mammals in mainland australia. antileptospiral agglutinins were detected in 20% of the sera tested and included nine l. interrogans serovars. the majority of serological r ... | 1998 | 9813843 |
| serosurvey of selected viral and bacterial diseases in wild swine from oklahoma. | blood samples collected from 120 wild swine (sus scrofa) in thirteen oklahoma (usa) counties during 1996 were tested for antibodies against six viral and two bacterial diseases. no antibodies to swine brucellosis, pseudorabies, transmissible gastroenteritis, and vesicular stomatitis were detected. antibody titers to one or more leptospiral serovars were found in 44% of the samples, the two most frequent serovars being leptospira interrogans serovars bratislava (29%) and pomona (27%). antibody ag ... | 1998 | 9813859 |
| cloned mammalian neutral sphingomyelinase: functions in sphingolipid signaling? | sphingomyelin is an abundant constituent of the plasma membranes of mammalian cells. ceramide, its primary catabolic intermediate, is released by either acid sphingomyelinase or neutral sphingomyelinase (nsmase) and has emerged as a potential lipid signaling molecule. nsmase is regarded as a key enzyme in the regulated activation of the "sphingomyelin cycle" and cell signaling. we report here the cloning, identification, and functional characterization of murine and human nsmase, a ubiquitously ... | 1998 | 9520418 |
| characterization of leptospiral outer membrane lipoprotein lipl36: downregulation associated with late-log-phase growth and mammalian infection. | we report the cloning of the gene encoding a 36-kda leptospiral outer membrane lipoprotein, designated lipl36. we obtained the n-terminal amino acid sequence of a staphylococcal v8 proteolytic-digest fragment in order to design an oligonucleotide probe. a lambda-zap ii library containing ecori fragments of leptospira kirschneri dna was screened, and a 2.3-kb dna fragment which contained the entire structural lipl36 gene was identified. several lines of evidence indicate that lipl36 is lipid modi ... | 1998 | 9529084 |
| unconventional genomic organization in the alpha subgroup of the proteobacteria. | pulsed-field gel electrophoresis was used to analyze the genomic organization of 16 bacteria belonging or related to the family rhizobiaceae of the alpha subgroup of the class proteobacteria. the number and sizes of replicons were determined by separating nondigested dna. hybridization of an rrn gene probe was used to distinguish between chromosomes and plasmids. members of the genus agrobacterium all possess two chromosomes, and each biovar has a specific genome size. as previously demonstrated ... | 1998 | 9573163 |
| structure and expression of the flaa periplasmic flagellar protein of borrelia burgdorferi. | the spirochete which causes lyme disease, borrelia burgdorferi, has many features common to other spirochete species. outermost is a membrane sheath, and within this sheath are the cell cylinder and periplasmic flagella (pfs). the pfs are subterminally attached to the cell cylinder and overlap in the center of the cell. most descriptions of the b. burgdorferi flagellar filaments indicate that these organelles consist of only one flagellin protein (flab). in contrast, the pfs from other spirochet ... | 1998 | 9573194 |
| detection of leptospira dna in patients with aseptic meningitis by pcr. | samples of cerebrospinal fluid from 103 patients with aseptic meningitis were tested by pcr for detection of leptospires, and the results were compared with those of the microscopic agglutination test (mat) and an enzyme-linked immunosorbent assay for detection of immunoglobulin m (elisa-igm). of these samples, 39.80% were positive by pcr and 8.74 and 3.88% were positive by mat and elisa-igm, respectively. | 1998 | 9574730 |
| leptospirosis in rehabilitated pacific harbor seals from california. | renal disease was observed in two rehabilitated pacific harbor seals (phoca vitulina richardsii) from a facility in california (usa). the seals had leukocytosis and high serum phosphorus, blood urea nitrogen and creatinine concentrations. a retrospective study of leptospiral antibody serum titers indicated both seals had elevated titers to leptospira interrogans serovar grippotyphosa. a third seal, which died about the time when the index cases occurred, also had elevated titers to l. interrogan ... | 1998 | 9577797 |
| leptospira genomics. | the bacterial species leptospira interrogans (sensu stricto) has a complex genome containing two circular chromosomal replicons. comparative analysis of the larger chromosome reveals a fluid genetic organization with many large rearrangements differentiating two closely related strains. in the present study new genes were identified by partial sequence analysis of randomly cloned fragments of l. interrogans dna. these genes were localized in regions of the genome by nucleic acid hybridization wi ... | 1998 | 9588807 |
| molecular characterization and postsplicing fate of three introns within the single rrna operon of the hyperthermophilic archaeon aeropyrum pernix k1. | the single rrna operon (arns-arnl) of the hyperthermophilic archaeon aeropyrum pernix k1 was sequenced. the dna sequence data and detailed rna analyses disclosed an unusual feature: the presence of three introns at hitherto undescribed insertion positions within the rrna genes. the 699-nucleotide (nt) intron ialpha was located at position 908 (escherichia coli numbering [h. f. noller, annu. rev. biochem. 53:119-162, 1984]) of the 16s rrna, while the 202-nt intron ibeta and 575-nt intron igamma w ... | 1998 | 9658008 |
| physical and genetic maps of the leptospira interrogans serovar icterohaemorrhagiae strain ictero no.1 chromosome and sequencing of a 19-kb region of the genome containing the 5s rrna gene. | we report the construction of physical and genetic maps of the chromosome of leptospira interrogans serovar icterohaemorrhagiae strain ictero no.1 using pulsed-field gel electrophoresis of dna fragments generated by digestion with enzymes srfi, asci, fsei, and noti and using reciprocal hybridization. we also sequenced the 19-kilobase (kb) dna segment including the one gene for 5s rrna (rrf) of pathogenic leptospira. the size of the chromosome of the strain ictero no.1 was estimated to be 4673kb ... | 1998 | 9666070 |
| morphological characterization of lung and kidney lesions in c3h/hej mice infected with leptospira interrogans serovar icterohaemorrhagiae: defect of cd4+ and cd8+ t-cells are prognosticators of the disease progression. | neonates and young c3h/hej mice were highly susceptible to lethal infection with leptospira interrogans serovar icterohaemorrhagiae. the main pathological changes were seen by light microscopy in the lung and kidneys of 3-week-old mice at 11 days after inoculation. lung histological lesions included small and medium-sized vasculitis with fibrinoid changes, hemorrhages, moderate infiltrate of mononuclear inflammatory cells and fibrin thrombi. in the kidney there was mild to severe acute tubular n ... | 1998 | 9681649 |
| physical and genetic map of the obligate intracellular bacterium coxiella burnetii. | pulsed-field gel electrophoresis and pcr techniques have been used to construct a noti macrorestriction map of the obligate intracellular bacterium coxiella burnetii nine mile. the size of the chromosome has been determined to be 2,103 kb comprising 29 noti restriction fragments. the average resolution is 72.5 kb, or about 3. 5% of the genome. experimental data support the presence of a linear chromosome. published genes were localized on the physical map by southern hybridization. one gene, rec ... | 1998 | 9683477 |
| transmission of leptospira interrogans serovar balcanica infection among socially housed brushtail possums in new zealand. | leptospira interrogans serovar balcanica is a potential vector being investigated for spreading a biological control agent among introduced brushtail possums (trichosurus vulpecula) in new zealand. as previous studies have shown that possums are unlikely to contract leptospirosis through a contaminated environment alone, the objective was to determine whether l. interrogans serovar balcanica could be transmitted between sexually mature, socially housed possums. possums were infected experimental ... | 1998 | 9706567 |
| molecular analysis of the dnak locus of leptospira interrogans serovar copenhageni. | analysis of the dnak locus of leptospira interrogans serovar copenhageni identified four genes in the order hrca, grpe, dnak and dnaj. this is the first time a homologue of hrca has been identified in a spirochete. the hrca gene and a regulatory sequence, designated circe, play a significant role in the regulation of the dnak locus of several gram+ organisms. their presence upstream of dnak in leptospira suggested a similiar regulatory mechanism. transcriptional analysis using reverse transcript ... | 1998 | 9714717 |
| a natural large chromosomal inversion in lactococcus lactis is mediated by homologous recombination between two insertion sequences. | comparative analysis of chromosomal macrorestriction polymorphism of the two closely related lactococcus lactis subsp. cremoris strains mg1363 and ncdo763 revealed the presence of a large inversion covering half of the genome. to determine what kind of genetic element could be implicated in this rearrangement, the two inversion junctions of mg1363 and ncdo763 chromosomes were cloned and characterized. nucleotide sequence analysis showed the presence of one copy of the lactococcal is905 element i ... | 1998 | 9733685 |
| epidemiologic features of equine leptospira interrogans of human significance. | leptospirosis is a zoonotic bacterial disease caused by leptospira interrogans. there is a serologic evidence that horses are exposed to l. interrogans and, as a shedder of these organisms, can be a threat to humans. we examined risk factors associated with the risk of testing seropositive to three l. interrogans serovars (l. icterohaemorrhagiae, l. grippotyphosa, and l. canicola) in the horses of new york state, in order to understand the epidemiology of the disease and suggest strategies to co ... | 1998 | 9762736 |
| first report of human leptospirosis due to leptospira interrogans serovar javanica in india. | 1998 | 9764329 | |
| macroscopic agglutination test for rapid diagnosis of human leptospirosis. | a commercially available slide agglutination test (sat) for the diagnosis of human leptospirosis was evaluated by comparing it to an immunoglobulin m (igm) enzyme-linked immunosorbent assay (elisa) and to the microscopic agglutination test (mat). for all 108 patients, leptospirosis was diagnosed on the basis of a fourfold or greater increase in titer by mat (seroconversion), and all but 1 of 245 controls were mat negative (titers, <1:100). both sat and the igm elisa failed to detect one case of ... | 1998 | 9774553 |
| identification and recombinant expression of a mycobacterium avium rhamnosyltransferase gene (rtfa) involved in glycopeptidolipid biosynthesis. | the mycobacterium avium complex is a source of disseminated infections in patients with advanced aids. this group of mycobacteria is distinguished by the presence of highly antigenic, surface-exposed glycopeptidolipids, and these glycolipids possess variant oligosaccharide structures that are the chemical basis of the 28 distinct serovars of the m. avium complex. we previously described the ser2 gene cluster, encoding the synthesis of the haptenic oligosaccharide (2, 3-dimethylfucose-rhamnose-6- ... | 1998 | 9791104 |
| prevalence and geographic origin of pigs with serological evidence of infection with leptospira interrogans serovar pomona slaughtered in abattoirs in victoria, australia. | a set of 10,440 sera was collected from pigs slaughtered at victorian abattoirs. these sera were subjected to the microscopic agglutination test for antibodies to leptospira interrogans serovar pomona. identification of the herd of origin was possible for 6511 pigs, and these were derived from 167 herds in victoria (84% of sera), from 32 herds in new south wales (8% of sera) and 29 herds in south australia (8% of sera). the overall prevalence of titres of 512 and above was 3.7%. this was higher ... | 1998 | 9791870 |
| [the effect of a vaccine made from 39kd hydrophobic outer membrane protein of leptospira interrogans on neurohumoral and red cell immunity function of the guinea pigs]. | a randomized control trial was conducted to determine the immunoprotective efficacy of ompl39. 36 guinea pigs were divided into ompl39 group, whole leptospiral cell vaccine (wlcv) group, other proteins of leptospira group, and negative control group (normal saline, ns). the results showed that all the guinea pigs of infected ompl39 and wlcv still survived, but all the control guinea pigs died. immunoprotective efficacy was 100% for ompl39 and wlcv. ompl39 levels were similar to wlcv levels and h ... | 1998 | 10683996 |
| [immunoprotection in guinea pigs using dna recombinant plasmid rpdjt and expressed protein p68 in l. interrogans serovar lai]. | immunoprotection against the infection by leptospira interrogans serogroup icterohemorrhagiae serovar lai strain 017 was demonstrated in guinea pigs vaccinated with dna recombinant plasmid rpdjt and expressed protein p68 derived from genomic library of leptospira strain 017. thirty days after active immunization, each group received intraperitoneally (1/2 dose) and subcutaneously (1/2 dose) inoculum of l. interrogans serovar lai stain 017; cultures were adjusted to 5 x 10(8) cells/ml. all guinea ... | 1998 | 10684084 |
| [cloning, characterization and expression in e. coli of the flagellin gene from leptospira interrogans serovar lai]. | to obtain a large amount of recombinant endoflagellin protein of leptospsira, we have cloned the fla b gene from leptospira interrogans serovar lai strain 017, which encodes the core flagellar protein. the entire open reading frame was amplified by polymerase chain reaction, which was digested with endonucleases sal i and cla i, then was orientationally cloned into the expression vector, pt7-7. the recombinant plasmid was transformed into escherichia coli jm109 (de3) for expression. after induct ... | 1998 | 10743226 |
| observations of leptospirosis in farmed deer. | slaughterhouse and on-farm surveys were undertaken to investigate some aspects of leptospirosis (leptospira interrogans) in farmed deer in the lower north island of new zealand. | 1998 | 16032037 |
| insertion sequences. | insertion sequences (iss) constitute an important component of most bacterial genomes. over 500 individual iss have been described in the literature to date, and many more are being discovered in the ongoing prokaryotic and eukaryotic genome-sequencing projects. the last 10 years have also seen some striking advances in our understanding of the transposition process itself. not least of these has been the development of various in vitro transposition systems for both prokaryotic and eukaryotic e ... | 1998 | 9729608 |
| effects of experimental infection with leptospira interrogans serovar balcanica on the health of brushtail possums (trichosurus vulpecula). | data on changes in the condition and feeding behaviour of captive brushtail possums (trichosurus vulpecula) following an experimental infection with leptospira interrogans serovar balcanica are presented. the daily food intake of nine experimentally infected possums was recorded for 47 days before and 24 days after balcanica inoculation (0.5 ml intraperitoneally containing about 10(8) organisms). body weight was recorded seven times between 42 days before and 96 days after inoculation. the mean ... | 1997 | 16031939 |
| [characterization of immunoblots of hydrophobic outer membrane proteins leptospira interrogans serovar lai strain 017]. | outer membrane proteins (omp) of leptospira interrogans serovar lai strain 017 were extracted by using triton x-114 (tx-114). the omp were solubilized and phase partitioned into both the hydrophilic, aqueous phase and hydrophobic, detergent phase. tx-114 did not solubilize the protoplasmic cylinder in intact organisms. the protoplasmic cylinders contained a lot of protein bands and most of the tx-114 solubilized proteins partitioned into the aqueous phase, whereas only 14 protein bands entered t ... | 1997 | 10683918 |
| [investigation on the rate of urinary excretion of leptospires among cattle naturally infected with leptospira interrogans]. | urine samples of leptospires from cattle were detected by polymerase chain reaction (pcr) and isolation. positive rates by southern-blot and agarose gel electrophoresis of pcr amplification productions were 13% and 11% respectively. positive rate of isolation was 3.1%. various positive rates by pcr for cattle urine from different areas were discovered. average rate of urinary excretion of leptospires among cattle that naturally infected with leptospira interrogans was 13.2%. data showed that cat ... | 1997 | 9812473 |
| differentiation of pathogenic and non-pathogenic leptospires by means of the polymerase chain reaction. | a polymerase chain reaction was carried out to detect pathogenic leptospires isolated from animals and humans in argentina. a double set of primers (g1/g2, b64-i/b64-ii), described before, were used to amplify by pcr a dna fragment from serogroups belonging to leptospira interrogans but did not allow to detect saprophytic strains isolated from soil and water (l. biflexa). this fact represents an advantage since it makes possible the differentiation of pathogenic from non-pathogenic leptospires i ... | 1997 | 9640782 |
| serological survey for diseases in free-ranging coyotes (canis latrans) in yellowstone national park, wyoming. | from october 1989 to june 1993, we captured and sampled 110 coyotes (canis latrans) for various diseases in yellowstone national park, wyoming (usa). prevalence of antibodies against canine parvovirus (cpv) was 100% for adults (> 24 months old), 100% for yearlings (12 to 24 months old), and 100% for old pups (4 to 12 months old); 0% of the young pups (< 3 months old) had antibodies against cpv. presence of antibodies against canine distemper virus (cdv) was associated with the age of the coyote, ... | 1997 | 9027690 |
| demonstration of leptospiral antigens on tissues using monoclonal antibodies and avidin-biotin peroxidase staining. | glycolipoprotein (glp) cytotoxin was extracted from leptospira interrogans serovar canicola. the silver staining profile of glp subjected to sds-page under denaturing conditions showed a number of bands in the mol. weight range of 14-66 kda. mouse monoclonal antibodies (mabs) igg3 recognizing a band near to 24 kda of leptospiral glp were produced (clone number mglp-01). the agglutinating property of mabs was established by microscopic agglutination test (mat) using 25 different serovars as antig ... | 1997 | 9495655 |