Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| transcription and translation maps of african swine fever virus. | a transcription map of african swine fever (asf) virus dna was obtained by hybridization of 32p-labeled early and late rnas synthesized in vero cells infected with asf virus to dot-blots containing cloned restriction fragments spanning the viral genome. early rnas synthesized in infected cells in the presence of protein or dna synthesis inhibitors hybridized preferentially to four regions in the genome, with coordinates e1 (0-51.9 kbp), e3 (63.7-75.2 kbp), e5 (100.1-111.6 kbp), and e7 (150-170 k ... | 1986 | 3716203 |
| glycosylated components of african swine fever virus particles. | extracellular african swine fever (asf) virus particles were specifically agglutinated by several lectins, suggesting the presence of surface glycosylated component(s) containing at least glucose, mannose, or both; galactose, n-acetylgalactosamine, or both; n-acetylneuraminic acid and n-acetylglucosamine, but not fucose. when virions were purified from infected vero cells labeled with [14c]glucosamine, [14c]galactose and analyzed by polyacrylamide gel electrophoresis, no major structural glycopr ... | 1986 | 3716204 |
| new agents active against african swine fever virus. | actinobolin, atropine, carrageenan, megalomycin c, suramin, and tetracenomycin c were tested for their activity against african swine fever virus replication. both viral inhibitory potency and cytotoxicity were investigated. megalomycin c, suramin, atropine, and carrageenan exhibited significant activity. megalomycin c was the most active of the four agents with respect to the concentration of compound that blocked the formation of infectious virus by 50%. suramin was the next most active agent ... | 1986 | 3717934 |
| african swine fever virus gene expression in infected vero cells. | polypeptides synthesized in vero cells infected with african swine fever virus (asfv) can be divided into early and late classes on the basis of their sensitivity to cytosine arabinoside. as asfv does not inhibit cell protein synthesis until late in infection, immunoprecipitation was used to identify virus-specific polypeptides. eighteen early and 15 late polypeptides were detected by polyacrylamide gel electrophoresis. early polypeptides can be further divided into those which are transiently e ... | 1986 | 3723111 |
| inhibition of african swine fever infection in the presence of immune sera in vivo and in vitro. | in assay cultures, sera from african swine fever convalescent pigs inhibited infection by homologous african swine fever virus. the infection-inhibition capacity did not correspond with the virus-neutralizing capacity. the serum did not prevent infection by heterologous virulent viruses. sera from pigs challenge inoculated with the homologous virulent virus and later with a heterologous virulent virus inhibited the infection by different heterologous virulent viruses. these sera did not interfer ... | 1986 | 3729125 |
| neutralization of african swine fever virus by sera from african swine fever-resistant pigs. | sera from african swine fever-resistant pigs with infection-inhibitory activity decreased virus replication in infected porcine buffy coat cultures. this same effect was observed even after virus was adsorbed. the infection-inhibition was not reversed by removing the immune serum from the assay cultures. reduction of african swine fever virus replication by immune sera was demonstrated by fluorescent focus assay on ms cell line cultures. virus-neutralization tests showed a persistent fraction of ... | 1986 | 3752695 |
| a novel selective broad-spectrum anti-dna virus agent. | a new compound has been found, (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine ((s)-hpmpa), that has potent and selective activity against a broad spectrum of dna viruses, including herpes simplex virus (types 1 and 2); varicella zoster virus; thymidine kinase-deficient (tk-) mutants of herpes simplex and varicella zoster virus; human cytomegalovirus; phocid, simian, suid, bovid and equid herpesviruses; african swine fever virus; vaccinia virus; and human adenoviruses. it is also active again ... | 1986 | 3762696 |
| african swine fever. i. morphological changes and virus replication in blood platelets of pigs infected with virulent haemadsorbing and non-haemadsorbing isolates. | replicating and mature viral particles were detected with the transmission electron microscope in blood platelets of pigs infected with virulent haemadsorbing and non-haemadsorbing african swine fever virus isolates. although platelet numbers decreased terminally in infected pigs, the most noticeable morphological damage to these cells apparent in the last 2 days of the disease included cytoplasmic swelling, vacuolation, fragmentation and loss of dense granules. | 1986 | 3763165 |
| hairpin loop structure of african swine fever virus dna. | the ends of african swine fever virus genome are formed by a 37 nucleotide-long hairpin loop composed, almost entirely, of incompletely paired a and t residues. the loops at each dna end were present in two equimolar forms that, when compared in opposite polarities, were inverted and complementary (flip-flop), as in the case of poxvirus dna. the hairpin loops of african swine fever and vaccinia virus dnas had no homology, but both dnas had a 16 nucleotide-long sequence, close to the hairpin loop ... | 1986 | 3763393 |
| single-stranded deoxyribonucleic acid nuclease induced by african swine fever virus and associated to the virion. | infection of vero cells with african swine fever (asf) virus resulted in a marked increase of dnase active on single-stranded dna (ss-dnase). no increase was observed for double-stranded dna-specific nuclease activity. in contrast to uninfected cell ss-dnase, which has a ph optimum at ph range 8.5-9, virus-induced ss-dnase is most active at ph 7. differences in sensitivity to several ions and other modifications of the reaction mixture and considerable difference in reaction kinetics suggest tha ... | 1986 | 3776099 |
| african swine fever virus-induced polypeptides in vero cells. | african swine fever virus induces in vero cells 81 acid and 14 basic polypeptides which account for most of the information content of the virus dna. the kinetics and the cytosine arabinoside sensitivity of the synthesis of the virus-induced polypeptides showed the existence of three classes of proteins, two early and one late. most of the early proteins were synthesized along the whole replication cycle, but the synthesis of some of the early proteins was switched-off after virus dna replicatio ... | 1986 | 3776350 |
| localization of structural proteins in african swine fever virus particles by immunoelectron microscopy. | seven african swine fever virus structural proteins were localized in the virion by immunoelectron microscopy. african swine fever virus-infected cells were incubated, before or after embedding and thin sectioning, with monoclonal antibodies specific for different structural proteins, and after labeling with protein a-gold complexes, the samples were examined in the electron microscope. proteins p14 and p24 were found in the external region of the virion, proteins p12, p72, p17, and p37 were fou ... | 1986 | 3517383 |
| two-dimensional analysis of african swine fever virus proteins and proteins induced in infected cells. | two-dimensional (2d) analysis of african swine fever (asf) virus purified by percoll gradient centrifugation resolves 54 structural proteins, 30 in conventional ief gels and 24 in nephge gels, while only 26 structural proteins are separated by sds-page. the two main bands separated by sds-page, with mol wt 150k and 72k, correspond to single spots in 2d gels. other bands, including major bands of 38k, 35k, 24k, 17k, and 15.5k mol wt, correspond to multiple proteins of the same molecular weight bu ... | 1986 | 3521071 |
| in vitro and in vivo association of african swine fever virus with swine erythrocytes. | the association of african swine fever virus (asfv) with swine erythrocytes in vivo, in high titers, was verified by inoculating 30 pigs with 17 asfv isolates and assaying their plasma and washed erythrocyte fractions for residual virus. viral antigens were specifically localized on the surface of in vitro and in vivo swine erythrocytes, using the fluorescent antibody technique and 3 monoclonal antibodies specific for asfv. the same monoclonal antibodies immunoprecipitated virus-specific polypep ... | 1986 | 3521406 |
| a micromethod for measuring swine fever antibody by neutralisation and immunofluorescence. | a micromethod employing the neutralisation and direct immunofluorescence technique for the detection of antibodies against swine fever in pig serum samples is described. the micromethod is simple and reproducible when compared with the macromethod. a total of 80 blood samples were randomly collected for comparison. the results are promising and reveal a 100 per cent correlation with the macrotechnique. | 1986 | 3526486 |
| identification of african swine fever viral antigens in the hemolymph of soft ticks (argasidae: ornithodoros) by the immunodot blot test. | the immunodot blot test was used to identify african swine fever virus (asfv) antigens in the hemolymph from soft ticks (ornithodoros coriaceus) fed on asfv-infected pigs. the immunodot blot test was sensitive and specific for asfv antigens and has potential field application. hemolymph from field-collected ticks can be screened for asfv and a variety of other tick-borne pathogens using this test. | 1986 | 3532842 |
| [differentiation of strains of the swine fever virus depending on their temperature resistance at 56 degrees c and on the size of fluorescent plaques]. | in vitro and in vivo experiments were carried out to differentiate the virulent swine fever virus strain vratsa and the vaccinal strains k and vp with regard to their resistance at 56 degrees c and to the size of the fluorescent plaques. the results obtained were comparable. the virulent strain was found to retain its viability at temperature of 56 degrees c for 30 min. the heated virus formed large, strongly fluorescent plaques in the infected cell cultures of pig kidney. the fluorescent cells ... | 1986 | 3544472 |
| diversity of african swine fever virus. | an african swine fever virus is an heterogeneous population, consisting of clones having different biological characteristics in respect to hemadsorption, virulence, infectivity, plaque size, and antigenic determinants. the following observations were made: nonhemadsorbing virus (nhv) have been segregated from field isolates from haiti (ht-1) and a bone marrow- and buffy coat-passaged portuguese isolate (l'60bm89bc1) and appear as a major, minor, or equal mixture with hemadsorbing viruses in the ... | 1985 | 2581483 |
| an immunological approach to vaccines against african swine fever virus. | until recently there were no published reports of any immunological mechanism which could curtail the replication of african swine fever virus (asfv). we have now described three such mechanisms--complement dependent lysis, antibody dependent cellular cytotoxicity and cytotoxic t lymphocytes. this paper discusses the likely role which each might play during asfv infection and indicates where this research might help with a disease where no effective vaccine is available. | 1985 | 3873757 |
| monoclonal antibodies specific for african swine fever virus proteins. | we have obtained 60 stable hybridomas which produced immunoglobulins that recognized 12 proteins from african swine fever virus particles and african swine fever virus-infected cells. most of the monoclonal antibodies were specific for the three major structural proteins p150, p72, and p12. the specificity of some monoclonal antibodies for the structural proteins p150 and p37 and the nonstructural proteins p220 and p60 indicated that proteins p150 and p220 are antigenically related to proteins p ... | 1985 | 3882998 |
| african swine fever virus. | 1985 | 3893908 | |
| purification and properties of african swine fever virus. | we describe a method for african swine fever (asf) virus purification based on equilibrium centrifugation in percoll density gradients of extracellular virions produced in infected vero cells that yielded about 15 +/- 9% recovery of the starting infectious virus particles. the purified virus preparations were essentially free of a host membrane fraction (vesicles) that could not be separated from the virus by previously described purification methods. the purified virus sedimented as a single co ... | 1985 | 3989907 |
| [swine fever: a changing clinical picture]. | signs of disease typical of classical swine fever are often absent in outbreaks caused by virus strains of low virulence. the clinical picture and epizootiology of these strains causing swine fever in the netherlands are elucidated on the basis of experimentally induced infections and outbreaks in the field respectively. strains of both high and low virulence are involved in the present epizootic. the absence of symptoms hampers early detection in the field. intra-uterine infections may occur in ... | 1985 | 3992564 |
| [commercial method of aerogenic immunization of pigs against swine fever]. | an industrial method is presented through a model with a chamber for aerogenic immunization of about 250 pigs, aged 2.5 months. the experiments were carried out with more than 1,000 pigs of the camborough hybrid under industrial conditions of raising. the process of immunization was feasibly carried out when the pigs passed through the chamber using aerosol at mixing them upon transference from the growing section to the fattening one, according to the provisions of the raising technology. a lap ... | 1985 | 3992924 |
| effect of chloroquine on african swine fever virus infection. | when present during the whole infective cycle, the lysosomotropic drug, chloroquine, inhibited cytopathic changes and production of african swine fever virus (asfv) in vero cells. this inhibition decreased when the drug was added from 1 h to 4 h after infection. chloroquine had no effect on the virus nor on viral adsorption and internalization. electron microscopy showed that, in the presence of the drug, the virions were retained in large vacuoles having a lysosomal appearance. this inhibition ... | 1985 | 3998709 |
| the role of antibody in protection against african swine fever virus. | intraperitoneal immunization of pigs with anti-african swine fever virus (asfv) antibody protected them against the effects of challenge with asfv. this protection, which was exemplified by a reduction in pyrexia and viraemia plus an increased survival time, appeared to be mediated through the effects of complement-dependent antibody-mediated cytotoxicity (cdac) or antibody dependent cell mediated cytotoxicity (adcc). experiments suggested that the reduction in viraemia was associated with compl ... | 1985 | 4035978 |
| mechanism of thrombocytopenia in african swine fever. | pigs were inoculated with an african swine fever (asf) isolate of moderate virulence, and the changes in the number of circulating blood platelets during infection were correlated with the appearance of antiviral antibody and fluctuations in total plasma hemolytic complement concentrations. thrombocytopenia was detected by postinoculation days (pid) 7 and 8, and antiviral antibody was detected by pid 7, using an indirect immunofluorescence technique. the total hemolytic complement concentration ... | 1985 | 4062007 |
| polypeptides and structure of african swine fever virus. | extracellular and intracellular african swine fever virus (asfv) was purified using a two-phase aqueous polymer system. both the structure of the virus and the polypeptides present during the purification procedure were studied. after peg/dextran phase separation and centrifugation through 20% (w/v) ficoll, 79% of input infectivity was recovered as semi-purified virus. the density of the virus after equilibrium centrifugation in sucrose was 1.19 g/ml. the envelope of the virion consisting of a u ... | 1985 | 4072397 |
| [onset and duration of immunity following aerogenic immunization of swine against swine pest and erysipelas]. | 1985 | 4074082 | |
| experimental transmission of african swine fever virus by ornithodoros savignyi (audouin). | the 'sand tampan', ornithodoros savignyi, is susceptible to oral infection with african swine fever (asf) virus in the laboratory. infected ticks can transmit the virus transstadially and are able to maintain it for at least 106 days. transmission of asf virus by infected ticks to healthy pigs was achieved on five separate occasions between 50 and 106 days after infection. pigs infected in this way developed typical acute african swine fever. the distribution of o savignyi in africa suggests tha ... | 1985 | 4081341 |
| [demonstration of the swine fever virus by the infection of cell cultures of lymphocytes and lysed red blood cells]. | a method was worked out for the diagnosing of swine fever by means of infecting sensitive cell cultures on lamellae with lyzed red blood cells and lymphocytes, the demonstration being performed through immunofluorescence. the new method made it possible to avoid killing of suspected animals for diagnostic purposes (in order to prepare suspensions of parenchymal organs and isolate the virus) while the characteristic morphologic changes were still not well manifested. | 1985 | 4090264 |
| morphological study on the entry of african swine fever virus into cells. | the early interactions between african swine fever virus (asfv) and monkey kidney cells in culture, and the effect of chloroquine were studied by electron microscopy. our results indicate that asfv uptake occurs by endocytosis: after attachment to the cell surface, the virions were seen in coated pits and were internalized by endocytosis in endosomes and finally in lysosomes. virions in coated vesicles were never seen. all these steps were completed in about 15 min. direct penetration of viruses ... | 1985 | 2937495 |
| [simultaneous vaccination against aujeszky's disease and european hog cholera]. | 1985 | 2986592 | |
| screening for neutralizing antibodies against hog cholera- and/or bovine viral diarrhea virus in danish pigs. | 1985 | 2994428 | |
| swine diseases transmissible with artificial insemination. | the transport of fresh and frozen semen to be used for artificial insemination creates a mode of disease transmission between farms. normally, semen contains a number of nonpathogenic bacterial contaminants; however, excessive bacterial contamination can result in infertile matings. contamination with a known pathogen, eg, brucella suis, could initiate a serious outbreak of disease in a recipient herd. methods to minimize bacterial contamination of semen include sanitary collecting and processin ... | 1984 | 6090372 |
| antiviral action of 5-amino-2-(2-dimethyl-aminoethyl)benzo-[de]-isoquinolin-1,3-dion e. | a newly synthesized imide derivative of 3-nitro-1,8-naphthalic acid, 5-amino-2-(2-dimethylaminoethyl)benzo-[de]-isoquinolin-1,3-dione (designated m-fa-142), was tested on chick embryo cells against herpes simplex virus type 1 (hsv-1) and vaccinia virus (vv), and on vero cells against african swine fever virus (asfv). at a concentration of 4 micrograms/ml the drug inhibited vv replication by about one order of magnitude, and that of hsv-1 by about three orders of magnitude. a minor effect was sho ... | 1984 | 6091540 |
| cytotoxic lymphocytes induced by african swine fever infection. | the generation of lymphocytes cytotoxic to african swine fever virus infected testis cells during in vivo infection is described. peripheral blood lymphocytes from 16 pigs developed cytotoxicity seven to eight days after infection but the lysis was not restricted to autologous cells. | 1984 | 6095388 |
| molecular cloning of african swine fever virus dna. | african swine fever virus dna (about 170 kbp) was cleaved with the restriction endonuclease ecori and most of the resulting 31 fragments were cloned in either the phage vector lambda wes lambda b or the plasmid pbr325. three fragments were not cloned in those vectors, the largest fragment ecori-a (21.2 kbp) and the two crosslinked terminal fragments, ecori-k' and d'. endonuclease sali cut fragment ecori-a into three pieces which were cloned in plasmid pbr322. the two terminal ecori fragments wer ... | 1984 | 6324451 |
| restriction site map of african swine fever virus dna. | treatment of african swine fever virus dna (about 170 kbp) with the restriction endonucleases sali, ecori, kpni, pvui, and smai yielded 14, 31, 17, 13, and 11 fragments, respectively. the order of the restriction fragments produced by each nuclease was established by identifying the crosslinked ecori and sali terminal fragments and then finding overlapping fragments. the five restriction fragment maps were integrated into a single map by locating sali, kpni, pvui, and smai sites in cloned ecori ... | 1984 | 6324452 |
| terminal and internal inverted repetitions in african swine fever virus dna. | an electron microscopic analysis of the heteroduplexes formed by reannealing denatured terminal restriction fragments of african swine fever (asf) virus dna showed y-shaped molecules with a 2.1-kilobase-pair-long double-stranded tail and two single-stranded arms. this indicated that asf virus dna has terminal inverted repetitions with a length of 2.1 kbp. in addition, under less restrictive hybridization conditions, most of the heteroduplexes showed a 0.13 kbp-long internal double-stranded regio ... | 1984 | 6324453 |
| quantitative aspects of the transmission of african swine fever. | the contagiousness of pigs during different stages of infection with african swine fever virus was assessed by measuring the amount of virus excreted and the amounts of virus in the blood and other tissues, as well as determining the infectious dose of the virus by various routes. the virus was present in substantial amounts in secretions and excretions of acutely infected pigs for only 7 to 10 days after the onset of fever and was present in the greatest amount in the feces. virus persisted in ... | 1984 | 6476567 |
| detection of antibodies against swine fever virus by enzyme-linked immunosorbent assay (elisa). | 1984 | 6524576 | |
| [hyperlipidemia in swine immunized with lapinized vaccine and live swine plague virus]. | the purpose of the studies was to show the relationships between secondary hyperlipaemia and post-vaccination nephritis induced by lapinized vaccine with additional infection with the living virus of pig plague and the virus itself. ct, cf, lt, ffa and lp fractions were studied in the serum of blood taken from the vena cava cranialis from piglets weighing 30 - 40 kg, of the large white breed. in the animals of group 1, in the initial series a, series b - immunized with lapinized vaccine, series ... | 1984 | 6537493 |
| the neutralizing peroxidase-linked assay for detection of antibody against swine fever virus. | the neutralizing peroxidase-linked antibody ( npla ) assay was standardized and compared with the micro-plaque reduction test (prt) on series of sera from pigs infected with different strains of swine fever virus (sfv) and bovine virus diarrhoea virus (bvdv), swine fever reference sera and field sera. the npla system was found to be as sensitive as the prt, it detected sfv antibody in 17 out of 18 pigs 3 weeks after intranasal exposure and differentiated between antibody against sfv and bvdv. wi ... | 1984 | 6375112 |
| porcine leukocyte cellular subsets sensitive to african swine fever virus in vitro. | african swine fever virus infected most, if not all, of the macrophages (monocytes) and ca. 4% of the polymorphonuclear leukocytes from porcine peripheral blood. b and t lymphocytes, either resting or stimulated with phytohemagglutinin, lipopolysaccharide, or pokeweed mitogen, were not susceptible to the virus. all of the mitogens used inhibited african swine fever multiplication in susceptible cells. the number of virus passages in vitro and the virulence degree of the virus did not affect the ... | 1984 | 6384555 |
| coagulation changes in african swine fever virus infection. | pigs were infected with highly virulent (tengani '62), with moderately virulent (dr '79) african swine fever (asf) virus, or with virulent hog cholera (hc) virus. changes in platelet counts, selected coagulation assays and concentrations of factor viii-related antigen (viiir:ag) were monitored. permeability of aortic endothelium was studied after the injection of evan's blue dye on various days after infection with dr '79 asf virus. virulent asf virus caused prolongation of the activated partial ... | 1984 | 6441489 |
| general morphology and capsid fine structure of african swine fever virus particles. | the structure of african swine fever virus particles has been examined by electron microscopy. the analysis of virions prepared by negative staining, thin sectioning, and freeze-drying and shadowing showed that the virus particle was composed of several concentric structures with an overall icosahedral shape. the inner region of the virus particles was a nucleoid that was surrounded by a membrane covered by the capsid. the capsid had side-to-side dimensions of 172 to 191 nm and was built up by c ... | 1984 | 6695498 |
| virulence in african swine fever: its measurement and implications. | a method of measuring and expressing the virulence of african swine fever virus in numerical terms was developed. seventeen viruses (13 hemadsorbing and 4 nonhemadsorbing) were tested and classified into 3 groups: highly infectious and highly virulent, highly infectious and moderately virulent, and slightly infectious and slightly virulent. this classification was based on the number of 50% hemadsorption unit (ha50) or tcid50 required to produce 1 ld50 for swine, the number of ha50 or tcid50 req ... | 1984 | 6711963 |
| clinical and immunologic responses of pigs to african swine fever virus isolated from the western hemisphere. | pigs in the united states were exposed to african swine fever (asf) virus isolated from pigs in brazil and the dominican republic. the former were examined for clinical response, lesions, viremia, and antibody response. sequential blood samples were tested for the presence of asf virus by the hemadsorption test (in swine buffy coat cell culture) and for antibody to asf virus by the enzyme-linked immunosorbent assay. the incubation period was 3 to 5 days; inoculated pigs had fever for 8 to 16 day ... | 1984 | 6731983 |
| characterization of african swine fever virus antigenic proteins by immunoprecipitation. | african swine fever virus is a large, complex virion in which numerous proteins have been identified by biochemical techniques. few of these proteins have been shown to react with antibodies from recovered swine, leading to speculation that the immunological unreactivity of some viral proteins might explain the inability of immune sera from surviving animals to neutralize the virus. we used immunoprecipitation of radiolabeled viral proteins to examine these sera in more detail. gradient sodium d ... | 1984 | 6732509 |
| proteins specified by african swine fever virus. iv. glycoproteins and phosphoproteins. | african swine fever virus infected ms cells labeled with radioactive 14c-amino acids, 32pi or [3h]-glucosamine were examined by high resolution sodium dodecylsulfate polyacrylamide gel electrophoresis and showed 43 infected cell polypeptides. twenty-one of these proteins were present in the nuclear fraction of infected cells. at least 22 of the infected cell polypeptides induced antibodies during natural infections in swine. the pattern of infected cell polypeptides modified by incorporation of ... | 1983 | 6416226 |
| congenital tremor type ai: light and electron microscopical observations on the spinal cords of affected piglets. | the histology and ultrastructure of the spinal white matter from the dorsolateral funiculus of the third cervical segment was studied in normal control pigs and pigs whose dams were inoculated with the weybridge congenital tremor strain of swine fever virus in early pregnancy. only inoculated sows produced abnormal piglets. these showed congenital tremors and ataxia. the severity of clinical signs was related to the degree of spinal myelin deficiency. morphologically this was quantified by deter ... | 1983 | 6841693 |
| effect of infections with swine fever virus on immune functions. ii. lymphocyte response to mitogens and enumeration of lymphocyte subpopulations. | peripheral blood and spleen lymphocytes from pigs infected with a low-virulent strain of swine fever virus (sfv) were transiently hyporesponsive to the mitogenic action of pha, pwm and con a. the mitogenic reactivity of lymphocytes from lymph nodes from such pigs appeared to be enhanced rather than depressed at that time. in addition, hyper-responsiveness of peripheral blood lymphocytes (pbl) to these mitogens occurred in some pigs. pbl from pigs lethally infected with virulent sfv showed a pers ... | 1983 | 6845636 |
| effect of infections with swine fever virus on immune functions. iii. antibody response to lipopolysaccharide and sheep red blood cells. | in order to determine the effect of infections with low-virulent swine fever virus (sfv) on antibody responses, pigs were administered lipopolysaccharide (lps) or sheep red blood cells (srbc), 2 days after infection. infected pigs showed an enhanced primary response to lps late during infection. the secondary response to lps seemed to be unaffected. both the primary and secondary antibody response to srbc appeared to be enhanced rather than depressed in infected pigs. these in vivo findings sugg ... | 1983 | 6845637 |
| interactions of porcine alveolar macrophages and bone marrow cells with african swine fever virus and virus-infected cells. | virus yields from porcine alveolar macrophages (am) infected with african swine fever virus (asfv) were greater and were achieved more rapidly, when inoculated at a high multiplicity of infection (moi) than at low moi. the difference was related to a lower percentage of cells becoming infected after low moi inoculation. the reduced yields after low moi were not caused by prolongation of the culture time, by bacterial endotoxins or by production of inhibitory substances by infected am. virus-infe ... | 1983 | 6868333 |
| investigation of porcine natural-killer cell activity with reference to african swine-fever virus infection. | the natural killing of the human myeloid leukaemia cell line by pig mononuclear cells was investigated in an 18 hr assay; the most active natural-killer (nk) effectors were those cells not adhering to nylon-wool columns or rosetting with sheep red blood cells. mononuclear cells cultured in the presence of african swine-fever virus maintained nk activity. pigs infected with african swine-fever virus exhibited a suppressed nk activity, possibly due to the sensitivity of nk cells to increased tempe ... | 1983 | 6575959 |
| effector mechanisms in the pig. antibody-dependent cellular cytolysis of african swine fever virus infected cells. | antibody dependent cellular cytolysis (adcc) against african swine fever virus infected nucleated cells was investigated in a porcine system. of the peripheral blood components examined, only neutrophils acted as effectors. lymph node derived cells displayed no adcc activity. in vitro yield reduction assays suggested that neutrophil mediated adcc may play a role in recovery from african swine fever virus infection. | 1983 | 6622849 |
| effect of rifamycin derivatives and coumermycin a1 on in vitro rna synthesis by african swine fever virus. brief report. | several rifamycin derivatives inhibited the dna-dependent rna polymerase of african swine fever (asf) virus particles. the inhibition was similar to that found with vaccinia virus rna polymerase. coumermycin a1, an inhibitor of type ii dna topoisomerases, inhibited strongly rna synthesis in vitro by asf virus particles. this suggests that transcription of asf virus dna requires a dna topoisomerase. | 1983 | 6625887 |
| swine fever. immunisation of piglets. | vaccination against swine fever using the cl chinese strain can be done in 7-day-old piglets if they are born of non-immune sows. the simultaneous weaning and vaccination emphasises the safety of this strain. the excellent immunity observed confirms the immunocompetence of 7-day-old piglets. in piglets born of immune sows and also weaned at 7 days, passive protection can extend beyond the age of 2 months if the sow is vaccinated several months prior to gestation. the immune level of the piglets ... | 1983 | 6671375 |
| replication of hog cholera virus in porcine alveolar macrophage cultures. | eight hog cholera viral strains were tested for virulence for pigs and ability to replicate in porcine alveolar macrophage cultures. of them, five were virulent and replicated well in the macrophage culture. the other three were avirulent and grew less remarkably in this culture than them. it was suggested that the ability of the hog cholera viral strains to replicate in porcine macrophages might be correlated with their virulence. | 1983 | 6677832 |
| pathophysiology of gravidity. | regulating processes during embryonal and fetal growth are discussed (genes, hormones). due to viral infections there can be disorder of fetal life (resorption, mumification, abortion and malformations). mucosal disease is hold up as an example to describe that it depends on the moment of infection, on the development of immunologic competence and on the qualities of the virus, what happens after infection. the development of immunologic competence is considered carefully. an infection with muco ... | 1983 | 6683837 |
| properties of hog cholera viruses recently isolated in japan. | hog cholera (hc) viruses newly isolated in japan in 1980 and 1981 were examined for pathogenicity and serological properties by the neutralization test with antisera against bovine viral diarrhea-mucosal disease (bvd . md) and hc viruses. five of 23 isolates examined were neutralized poorly by bvd . md antibody, but well by hc antibody. on the contrary, 15 isolates were neutralized readily and two isolates moderately by bvd . md antibody. the other one reacted poorly with either hc or bvd . md a ... | 1983 | 6330573 |
| [enhancement of the immune response by aspecific action of vaccine additives in the aerogenic immunization of swine against swine plague]. | 1983 | 6362602 | |
| african swine fever virus. brief review. | 1983 | 6307224 | |
| congenital infections with nonarbo togaviruses. | the present review deals with the similarities and differences of selected aspects of prenatal pestivirus infections of domestic animals and congenital rubella of man. hog cholera virus, bovine virus diarrhoea virus and border disease virus are antigenically closely related, but unrelated to rubella virus. the nonarbo togaviruses are capable of producing congenital infections resulting in a wide spectrum of abnormalities. the infected foetus can die in utero, in the neonatal period, or it may be ... | 1983 | 6314636 |
| could aids agent be a new variant of african swine fever virus? | 1983 | 6132234 | |
| african swine fever virus antibody not found in aids patients. | 1983 | 6133121 | |
| new method of antibody detection by indirect immunoperoxidase plaque staining for serodiagnosis of african swine fever. | an indirect immunoperoxidase plaque-staining method was developed for detecting antibody to african swine fever virus infection. in both sensitivity and specificity, the test was comparable to indirect immunofluorescence. because it has all of the desirable features of the indirect immunofluorescence test and may also be readily used for testing large numbers of sera, the indirect immunoperoxidase plaque-staining method can be used as a single and final serodiagnostic test in a large-scale surve ... | 1982 | 6185528 |
| effect of african swine fever on lymphocyte mitogenesis. | the effect of african swine fever virus replication on t and b lymphocytes was studied by mitogen-driven assays. live attenuated isolates caused a marked suppressive effect which was dose- and time-dependent. this effect appeared to be mediated through a monokine. live virulent isolates enhanced lymphocyte mitogenesis in most cases, with increased [3h]-thymidine uptake by t cells and increased ig secretion by b cells. killed preparation had no effect. these results are discussed in the light of ... | 1982 | 6210625 |
| [african swine plague in brazil: 3 years of laboratory study]. | 1982 | 6765357 | |
| african swine fever virus dna: restriction endonuclease cleavage patterns of wild-type, vero cell-adapted and plaque-purified virus. | dna from african swine fever (asf) virus was isolated and was characterized by two restriction enzymes, smai and ecori. although both enzymes can distinguish vero cell-adapted asf isolates by characteristic restriction endonuclease cleavage patterns, all asf isolates examined exhibited a high degree of similarity, as measured by co-migration of most of the dna fragments. the molecular weight of asf dna, based on size estimates of dna fragments from cleavage patterns, ranged from 93 x 10(6) to 10 ... | 1982 | 6296285 |
| [effect of specific vaccinal antigens and preparations on general resistance in growing experimental and domestic animals]. | studied was the effect of some viral and bacterial antigens as well as of a preparation obtained by filatov's method (modified by the author) on the general resistance in growing laboratory and domestic animals. it was found that in infantile albino mice the best protection against challenge with escherichia coli and pasteurella avicida was provided through the treatment with a biostimulator and a killed culture of a strongly proteolytic, unidentified strain of the 't3' bacterium. the vaccines a ... | 1982 | 6820584 |
| end-point dilution-fluorescent antibody technique for cloning hog cholera virus. | hog cholera virus was cloned by incubating selected pretitrated dilutions of the virus on pk-15 cell cultures for 2 hours. after a thorough washing, the coverslip cell cultures were overlaid with medium containing 0.1% hog cholera immune serum to prevent secondary foci. forty-eight hours later, the cultures were vigorously washed and maintenance medium containing 5% bovine fetal serum was added. when examined by the fluorescent antibody technique 18 hours later, single plaques were observed in s ... | 1982 | 7041710 |
| inactivation of african swine fever virus in tissues by gamma radiation. | 1982 | 7046538 | |
| complement-mediated lysis of african swine fever virus-infected cells. | using an homologous pig system, th lysis of african swine fever virus-infected cells by antibody and complement was investigated. the optimal conditions necessary for lysis are described, and it was found that the system was unique amongst reported virus infections in that infected cells were lysed by the classical complement pathway and not the alternative pathway. development of antibody capable of initiating complement-mediated lysis was relatively late in the infected pig, although functiona ... | 1982 | 7076281 |
| production and titration of african swine fever virus in porcine alveolar macrophages. | the broncho-alveolar lavage of a pig (20-40 kg) contains about 1.6 x 10(9) alveolar cells, half of which were macrophages. the number of cells in the lavage of bacille calmette guerin (bcg)-treated pigs increased about 4-fold. both african swine fever virus-infected porcine alveolar macrophages and blood monocytes produced about 1000 hemadsorption units/cell, a value 10-fold larger than that obtained in virus-infected vero cells. porcine alveolar cells could be stored frozen and, after thawing, ... | 1982 | 7085838 |
| [relevance of particle size for aerogenic immunization]. | 1982 | 7138219 | |
| [onset and persistence of immunity to swine fever following aerogenic immunization]. | 1982 | 7138220 | |
| in vivo labelling of viral proteins with 75selenomethionine. | a method is described for in vivo labelling of viral proteins with a gamma ray emitting radionuclide. the amino acid methionine labelled with 75se instead of 35s will be accepted by cells in culture for protein synthesis. a high specific labelling of viral proteins is achieved which allows rapid detection of virus or viral proteins in gradient centrifugation and page as well as further biochemical or serological analysis of the proteins. as an example hog cholera virus (family togaviridae) was l ... | 1982 | 7153307 |
| [african swine plague: study of viral resistance to the action of crystal violet]. | 1982 | 6985409 | |
| [ultramicroscopic observations of african swine plague virus in swine erythrocytes]. | 1982 | 6985411 | |
| congenital tremor in pigs farrowed from sows given hog cholera virus during pregnancy. | at different stages of gestation, 3 groups of pregnant sows were inoculated with a strain of hog cholera virus (hcv). after the infection, clinical signs of hog cholera were not observed in the sows. pigs from the sows infected on day 22 or 43 of gestation showed varying degrees of muscular tremor, ataxia, splayleg, and suckling inability. of the pigs with tremor, 83% had cerebellar hypoplasia. surviving pigs demonstrated persistent viral infection and continued to shed hcv, but did not have ant ... | 1981 | 7224307 |
| possible factors influencing immunoglobulin a concentration in swine colostrum. | the immunoglobulin (ig) a concentration in swine colostrum was determined by the single radial immunodiffusion method, using 157 samples collected from the same number of farm-raised sows in the yamaguchi prefecture of japan during 1976 and 1977. the mean iga value was 12.26 +/- 3.30 mg/ml, and the maximum and minimum values were 28.14 mg/ml and 5.63 mg/ml, respectively. to determine factors influencing the iga concentration in swine colostrum, the following items were analyzed in the present st ... | 1981 | 7271021 |
| a simple procedure for the concentration and purification of hog cholera virus (hcv) using the lectin of ricinus communis. | 1981 | 7295042 | |
| [use of the hemadsorption method to demonstrate the african swine fever virus in angola]. | attempts were made to modify the hemadsorption method used in the demonstration of the african swine fever virus. use was made of fourteen strains of the virus with known hemadsorption properties and infectious titers, which were replicated in leukocyte cultures of swine blood, in three variants: (a) at direct infection of the cultures without changing the medium; (b) at infecting with changing the medium and adding erythrocytes to the new medium; and (c) infecting the cultures with changing the ... | 1981 | 7303482 |
| micro method for performing titration and neutralization test of hog cholera virus using established porcine kidney cell strain. | hog cholera (hc) virus and antibody against it were estimated by the end method with microplates and cpk porcine kidney cell strain. to establish the technique of this method, studies were made on such basic conditions of the method as the type of strain of newcastle disease virus (ndv), the time of challenge with this virus, and the concentration of serum in culture fluid. there was little difference in the infective titer of hc virus estimated between the end method performed by the establishe ... | 1981 | 7200574 |
| lymphocyte function and cell-mediated immunity in pigs with experimentally induced african swine fever. | twenty-five pigs inoculated with african swine fever virus were studied for the development of changes in lymphocyte numbers and function. the results indicated that lymphopenia and decreased percentage of circulating t-lymphocytes occurred as early as 7 days after the pigs were inoculated. these were accompanied by depressed lymphocyte function, as measured by mitogen- or antigen-induced blastogenesis. there were proportionately greater decreases in t-lymphocyte numbers and function than were f ... | 1981 | 6975049 |
| detection of antibodies against hog cholera virus and bovine viral diarrhea virus in porcine serum. a comparative examination using cf, pla and npla assays. | 1981 | 6266240 | |
| differences in reaction behaviour of structural polypeptides of bovine viral diarrhoea virus (bvdv) with antisera against bvdv and hog cholera virus (hcv). | 1981 | 6269332 | |
| nucleoside triphosphate phosphohydrolase activities in african swine fever virus. | african swine fever virus contains nucleoside triphosphate phosphohydrolase activity which releases 32p phosphate from gamma-32p atp at a rate of about 1 mumol/h mg of virus protein. the hydrolase activity is slightly stimulated by adding nucleic acids to the reaction mixture and under conditions of rna synthesis. a study of the rate of atp hydrolysis at different concentrations of atp suggests the existence of two phosphohydrolase activities with apparent km values of about 0.04 and 1 mm. | 1981 | 6271106 |
| hog cholera antibodies in pigs vaccinated with an aujeszky-vaccine based on antigen produced in ib-rs-2 cells. | 1981 | 6283854 | |
| polyadenylation, methylation, and capping of the rna synthesized in vitro by african swine fever virus. | 1981 | 6168100 | |
| leukocyte-dependent platelet vasoactive amine release and immune complex deposition in african swine fever. | fifteen pigs were inoculated with african swine fever virus in a study of the pathogenesis of the disease. all pigs surviving the first two weeks developed high circulating antibody titers against african swine fever virus and persistent viremia. hemolytic complement levels declined to 50 to 70 hemolytic complement 50 (ch50) units/ml from mean preinoculation levels of 120 ch50 units/ml. immune deposits consisting of african swine fever antigen, host immunoglobulin g, and native c3 were found in ... | 1981 | 6170156 |
| induced african swine fever in feral pigs. | feral pigs trapped in florida were exposed by intranasal/oral inoculation or contact to african swine fever virus isolants from the iberian peninsula and the dominican republic. all exposed pigs became sick and died or were killed after becoming moribund. necropsy revealed lesions typical of african swine fever, but several pigs had marked hemorrhage surrounding the kidneys or rectum or in the gastric, hepatic, or ventral abdominal region that, in the field, might have suggested trauma or poison ... | 1981 | 7309606 |
| [study on hog cholera-swine erysipelas combined live vaccine. -iii. field trial of vaccination (author's transl)]. | 1981 | 7324808 | |
| experimental transplacental transmission of hog cholera virus in pigs. iv. virological and serological studies in newborn piglets. | 1981 | 7336835 | |
| experimental transplacental transmission of hog cholera virus in pigs. v. immunopathological findings in newborn pigs. | 1981 | 7342578 | |
| in vitro study of the cellular response of pigs vaccinated against classical swine fever. | 1981 | 7342581 | |
| african swine fever virus (malta/78) in pigs. | 1981 | 7345110 | |
| field evaluation of enzyme-linked immunosorbent assay for detection of antibody to african swine fever virus. | the enzyme-linked immunosorbent assay (elisa) for detection of antibodies to african swine fever virus was evaluated under field conditions in the dominican republic. a total of 3,402 swine serums were tested by elisa. all elisa-positive serums (n = 224) and 426 of 2,760 elisa-negative serums were retested by indirect immunofluorescence (iif). there were no antibodies to asf virus detected by iif in elisa-negative serums. by iif, antibodies were detected in only a small portion of the elisa-posi ... | 1981 | 7027849 |