Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| theoretical study of interactions between muscle aldolase and f-actin: insight into different species. | interactions of the glycolytic enzyme, fructose-1,6-bisphosphate aldolase (aldolase), with f-actin may be one mechanism for the colocalization of glycolytic enzymes. examination of these interactions in different animal species tests this hypothesis by observing whether binding sites are conserved across species. brownian dynamics (bd) simulations provide descriptions of such protein-protein interactions with the muscle isoforms of zebra fish and human aldolase. the results are compared with pre ... | 2007 | 17039493 |
| chemical genomic profiling for identifying intracellular targets of toxicants producing parkinson's disease. | the yeast deletion collection includes approximately 4700 strains deleted for both copies of every nonessential gene. this collection is a powerful resource for identifying the cellular pathways that functionally interact with drugs. in the present study, the complete pool of approximately 4700 barcoded homozygous deletion strains of saccharomyces cerevisiae were surveyed to identify genes/pathways interacting with 1-methyl-4-phenylpyridinium (mpp(+)) and n,n-dimethyl-4-4-bipiridinium (paraquat) ... | 2007 | 17043098 |
| impact of transcriptional properties on essentiality and evolutionary rate. | we characterized general transcriptional activity and variability of eukaryotic genes from global expression profiles of human, mouse, rat, fly, plants, and yeast. the variability shows a higher degree of divergence between distant species, implying that it is more closely related to phenotypic evolution, than the activity. more specifically, we show that transcriptional variability should be a true indicator of evolutionary rate. if we rule out the effect of translational selection, which seems ... | 2007 | 17057246 |
| hpv-18 e7 conjugates to c-myc and mediates its transcriptional activity. | several reports in the literature have indicated that the e6 not only elevates the level of c-myc level but that the protein also associates with the myc complex and activates myc-responsive genes. there would seem to be a mechanism by which this oncogene can modulate cell proliferation and differentiation. furthermore, an increase in c-myc levels has also observed during ectopic expression of hpv e7 alone. using the yeast two-hybrid system, we further found that the c-myc interacts and forms a ... | 2007 | 17070091 |
| securin induces genetic instability in colorectal cancer by inhibiting double-stranded dna repair activity. | genetic instability (gi) is a hallmark feature of tumor development. securin, also known as pituitary tumor transforming gene (pttg), is a mitotic checkpoint protein which is highly expressed in numerous cancers, is associated with tumor invasiveness, and induces gi in thyroid cells. we used fluorescence inter-simple sequence repeat pcr to assess gi caused primarily by dna breakage events in 19 colorectal tumors. gi values ranged significantly, with dukes' stage c&d colorectal tumors exhibiting ... | 2007 | 17071631 |
| purification and properties of glutathione reductase from liver of the anoxia-tolerant turtle, trachemys scripta elegans. | glutathione reductase (gr) is a homodimeric flavoprotein that catalyzes the reduction of oxidized glutathione (gssg) using nadph as a cofactor. the enzyme is a major component of cellular defense mechanisms against oxidative injury. in this study, gr was purified from the liver of the anoxia-tolerant turtle, trachemys scripta elegans. the overall fold purifications were 13.3- and 12.1-fold with final specific activities of 5.5 and 1.44 u/mg of protein for control and anoxic turtle gr, respective ... | 2007 | 17075686 |
| the amino-conserved domain of human cytomegalovirus ul80a proteins is required for key interactions during early stages of capsid formation and virus production. | assembly of many spherical virus capsids is guided by an internal scaffolding protein or group of proteins that are often cleaved and eliminated in connection with maturation and incorporation of the genome. in cytomegalovirus there are at least two proteins that contribute to this scaffolding function; one is the maturational protease precursor (pul80a), and the other is the assembly protein precursor (pul80.5) encoded by a shorter genetic element within ul80a. yeast gal4 two-hybrid assays esta ... | 2007 | 17079329 |
| the nucleolar protein surf-6 is essential for viability in mouse nih/3t3 cells. | surf-6 is a bona fide nucleolar protein comprising an evolutionary conserved family that extends from human to yeast. the expression of the mammalian surf-6 has been recently found to be regulated during the cell cycle. in order to determine the importance of surf-6 in mammalian cells, we applied the tet-on system to regulate conditionally, in response to tetracycline, the expression of an antisense rna (asrna) that targets surf-6 mrna in mouse nih/3t3 cells. induced surf-6 asrna caused an effec ... | 2007 | 17086444 |
| irreversible inhibition of glucose-6-phosphate dehydrogenase by the coenzyme a conjugate of ketoprofen: a key to oxidative stress induced by non-steroidal anti-inflammatory drugs? | oxidative damage by non-steroidal anti-inflammatory drugs (nsaids) has been considered relevant to the occurrence of gastro-intestinal side-effects. in the case of chiral arylpropionate derivatives like ketoprofen (kpf), this mechanism has been evidenced for the r-enantiomer, especially when chiral inversion was observed, and lets us suppose the involvement of coa conjugates. glucose-6-phosphate dehydrogenase (g6pd) is the crucial enzyme to regenerate the gsh pool and maintain the intracellular ... | 2007 | 17094951 |
| 2,5-dimethyl-4-hydroxy-3(2h)-furanone (dmhf); antimicrobial compound with cell cycle arrest in nosocomial pathogens. | 2,5-dimethyl-4-hydroxy-3(2h)-furanone (dmhf), an aroma compound found in a number of fruits and foods, has shown various biological properties in animal models, but its antimicrobial effect remains poorly understood. the current study investigated the antimicrobial effect of dmhf using human pathogenic microorganisms including clinically isolated antibiotics-resistant strains. the results indicated that dmhf exhibited broad spectrum antimicrobial activities in an energy-dependent manner without ... | 2007 | 17097685 |
| ubiquitin receptor proteins hhr23a and hplic2 interact. | ubiquitin receptor proteins play an important role in delivering ubiquitylated protein substrates to the proteasome for degradation. hhr23a and hplic2 are two such ubiquitin receptors that contain ubiquitin-like (ubl) domains, which interact with the proteasome, and ubiquitin-associated (uba) domains, which interact with ubiquitin. depending on their abundance ubl/uba family members can either promote or inhibit the degradation of other proteins, which suggests their participation in the deliver ... | 2007 | 17098253 |
| esldb: eukaryotic subcellular localization database. | eukaryotic subcellular localization database collects the annotations of subcellular localization of eukaryotic proteomes. so far five proteomes have been processed and stored: homo sapiens, mus musculus, caenorhabditis elegans, saccharomyces cerevisiae and arabidopsis thaliana. for each sequence, the database lists localization obtained adopting three different approaches: (i) experimentally determined (when available); (ii) homology-based (when possible); and (iii) predicted. the latter is com ... | 2007 | 17108361 |
| conditional stimulation of type v and vi adenylyl cyclases by g protein betagamma subunits. | in a yeast two-hybrid screen of mouse brain cdna library, using the n-terminal region of human type v adenylyl cyclase (hacv) as bait, we identified g protein beta2 subunit as an interacting partner. additional yeast two-hybrid assays showed that the gbeta(1) subunit also interacts with the n-terminal segments of hacv and human type vi adenylyl cyclase (hacvi). in vitro adenylyl cyclase (ac) activity assays using membranes of sf9 cells expressing hacv or hacvi showed that gbetagamma subunits enh ... | 2007 | 17110384 |
| a simple model system for age-dependent dna damage and cancer. | aging is the major risk factor for many human cancers. however, the mechanisms responsible for the effect of aging on tumor incidence are poorly understood, in part because few model systems are available to study age-dependent genomic instability. furthermore, the role of dna mutations in "normal aging" and "life span extension" is unclear. our laboratory has developed a novel method to study aging in yeast based on the survival of non-dividing populations (chronological life span). two major p ... | 2007 | 17118426 |
| internalization and molecular interactions of human cd21 receptor. | the human cd21 is a receptor for cleavage fragments of the third complement component and for epstein-barr virus. previous mutational studies showed that the cytoplasmic domain of cd21 is absolutely required for internalization of either ligand. with the exception of cd19, cd81, leu-13 and cd35 that can form a complex with cd21 at the cell surface, no other partner that interacts with the hcd21 transmembrane or the cytoplasmic domain was identified. we investigated the internalization capacity o ... | 2007 | 17118449 |
| the subcellular localisation of trypanosome rrp6 and its association with the exosome. | the exosome, a complex of 3'-exoribonucleases and associated proteins, is involved in the degradation of eukaryotic mrnas in the cytoplasm, and has rna processing and quality control functions in the nucleus. in yeast, the nuclear exosome differs from the cytoplasmic one in that it contains an additional non-essential component, rrp6p. in contrast, a small proportion of human rrp6 has been shown to localise to the cytoplasm as well. when we purified the trypanosoma brucei exosome from cytosolic ... | 2007 | 17118470 |
| interaction of the ca2+-sensing receptor with the inwardly rectifying potassium channels kir4.1 and kir4.2 results in inhibition of channel function. | the ca(2+)-sensing receptor (car), a g protein-coupled receptor, is expressed in many epithelial tissues including the parathyroid glands, kidney, and gi tract. although its role in regulating pth levels and ca(2+) metabolism are best characterized, it may also regulate salt and water transport in the kidney as demonstrated by recent reports showing association of potent gain-of-function mutations in the car with a bartter-like, salt-wasting phenotype. to determine whether this receptor interact ... | 2007 | 17122384 |
| arc1p is required for cytoplasmic confinement of synthetases and trna. | in yeast, arc1p interacts with scmetrs and scglurs and operates as a trna-interacting factor (tif) in trans of these two synthetases. its n-terminal domain (n-arc1p) binds the two synthetases and its c-terminal domain is an emapii-like domain organized around an ob-fold-based tif. arc1 is not an essential gene but its deletion (arc1- cells) is accompanied by a growth retardation phenotype. here, we show that expression of n-arc1p or of c-arc1p alone palliates the growth defect of arc1- cells, an ... | 2007 | 17131041 |
| improved yields of full-length functional human fgf1 can be achieved using the methylotrophic yeast pichia pastoris. | we have produced human fibroblast growth factor 1 (hfgf1) in the methylotrophic yeast pichia pastoris in order to obtain the large amounts of active protein required for subsequent functional and structural characterization. four constructs were made to examine both intracellular and secreted expression, with variations in the location of the his6 tag at either end of the peptide. hfgf1 could be produced from all four constructs in shake flasks, but production was optimized by growing only the h ... | 2007 | 17134911 |
| peroxisomedb: a database for the peroxisomal proteome, functional genomics and disease. | peroxisomes are essential organelles of eukaryotic origin, ubiquitously distributed in cells and organisms, playing key roles in lipid and antioxidant metabolism. loss or malfunction of peroxisomes causes more than 20 fatal inherited conditions. we have created a peroxisomal database (http://www.peroxisomedb.org) that includes the complete peroxisomal proteome of homo sapiens and saccharomyces cerevisiae, by gathering, updating and integrating the available genetic and functional information on ... | 2007 | 17135190 |
| cytoskeletal structures, ultrastructural characteristics and the capsule of the basidiomycetous yeast cryptococcus laurentii. | the cytoskeleton, capsule and cell ultrastructure were studied during the cell cycle of cryptococcus laurentii. in an encapsulated strain, cytoplasmic microtubules and a mitotic spindle were detected. mitosis was preceded by migration of the nucleus into the bud. f-actin failed to be visualised by rhodamine-phalloidin (rhph) in encapsulated cells and therefore an acapsular strain was used. the following actin structures were found: actin dots, actin cables and cytokinetic ring. ultrastructural s ... | 2007 | 17136567 |
| functional studies of aldo-keto reductases in saccharomyces cerevisiae. | we utilized the budding yeast saccharomyces cerevisiae as a model to systematically explore physiological roles for yeast and mammalian aldo-keto reductases. six open reading frames encoding putative aldo-keto reductases were identified when the yeast genome was queried against the sequence for human aldose reductase, the prototypical mammalian aldo-keto reductase. recombinant proteins produced from five of these yeast open reading frames demonstrated nadph-dependent reductase activity with a va ... | 2007 | 17140678 |
| mammalian timeless and tipin are evolutionarily conserved replication fork-associated factors. | the function of the mammalian timeless protein (tim) has been enigmatic. tim is essential for early embryonic development, but little is known regarding its biochemical and cellular function. although identified based on similarity to a drosophila circadian clock factor, it also shares similarity with a second family of proteins that is more widely conserved throughout eukaryotes. members of this second protein family in yeast (s.c. tof1p, s.p. swi1p) have been implicated in dna synthesis, s-pha ... | 2007 | 17141802 |
| role of protein o-mannosyltransferase pmt4 in the morphogenesis and virulence of cryptococcus neoformans. | protein o mannosylation is initiated in the endoplasmic reticulum by protein o-mannosyltransferases (pmt proteins) and plays an important role in the secretion, localization, and function of many proteins, as well as in cell wall integrity and morphogenesis in fungi. three pmt proteins, each belonging to one of the three respective pmt subfamilies, are encoded in the genome of the human fungal pathogen cryptococcus neoformans. disruption of the c. neoformans pmt4 gene resulted in abnormal growth ... | 2007 | 17142566 |
| cellular expression, localization and interactions of the product of the human most-1 gene associated with breast and prostate cancers. | we previously isolated and characterized the novel human gene most-1 (c8orf17) that is ubiquitously expressed in all cancer cell lines tested but differentially expressed in normal adult tissues. most-1 maps to chromosome region 8q24.2 whose amplification is frequently associated with breast and prostate cancers. rt-pcr analyses of breast and prostatic biopsies revealed most-1 overexpression and/or amplification in high-grade carcinomas. we raised and characterized a polyclonal antibody against ... | 2007 | 17143515 |
| inhibitory effect of umbelliferone aminoalkyl derivatives on oxidosqualene cyclases from s. cerevisiae, t. cruzi, p. carinii, h. sapiens, and a. thaliana: a structure-activity study. | eighteen coumarin derivatives were tested as inhibitors of oxidosqualene cyclases (oscs) from saccharomyces cerevisiae, trypanosoma cruzi, pneumocystis carinii, homo sapiens, and arabidopsis thaliana, all expressed in an osc-defective strain of s. cerevisiae.35 all the compounds have an aminoalkyl chain bound to an aromatic nucleus; unconventional synthetic procedures (microwave- and ultrasound-promoted reactions) were successfully used to prepare some of them. the most interesting structure-dep ... | 2007 | 17154325 |
| searching for sequence directed mutagenesis in eukaryotes. | sequence directed mutagenesis is a mechanism by which imperfect repeats "repair" each other to become perfect, generating mutations. this process is known to be prevalent in prokaryotes and it has been implicated in several human genetic diseases. here we test whether sequence directed mutagenesis occurs in the protein coding sequences of eukaryotes using extensive dna sequence data from humans, mice, drosophila, nematodes, yeast, and arabidopsis. using two tests we find little evidence of seque ... | 2007 | 17160648 |
| differential regulation of splicing, localization and stability of mammalian ard1235 and ard1225 isoforms. | ard1 protein is a mammalian gene product homologous to a yeast ard1p (arrest defective 1 protein) acetyltransferase. although two alternative splicing products of ard1, ard1(235) and ard1(225), were reported in mouse, only ard1(235) orthologue was reported in humans. here we show that ard1(225) is not expressed in humans, suggesting that factors regulating alternative splicing of ard1 may have evolved differently between species. in human cells, hard1(235) is shown to be present in both nucleus ... | 2007 | 17161380 |
| mitochondrial topoisomerases and alternative splicing of the human top1mt gene. | mitochondria are the only organelles containing metabolically active dna besides nuclei. by analogy with the nuclear topoisomerases, mitochondrial topoisomerase activities are probably critical for maintaining the topology of mitochondrial dna during replication, transcription, and repair. mitochondrial diseases include a wide range of defects including neurodegeneracies, myopathies, metabolic abnormalities and premature aging. vertebrates only have one known specific mitochondrial topoisomerase ... | 2007 | 17161897 |
| [a pichia pastoris with alpha-1, 6-mannosyltransferases deletion and its use in expression of hsa/gm-csf chimera]. | yeast is a widely used host for recombinant protein expression. however, glycoproteins derived from yeast contain n-glycan of high mannose type and are usually hyperglycosylated. alpha-1,6-mannosyltransferases gene (och1) encodes the enzyme that initiates the first step of out-chain elongation of high mannose type n-glycan in yeast, which is different from that in human. so, a high efficient method to knockout target gene by two-step recombination was established and was used to delete och1. in ... | 2007 | 18051874 |
| parameters of proteome evolution from histograms of amino-acid sequence identities of paralogous proteins. | the evolution of the full repertoire of proteins encoded in a given genome is mostly driven by gene duplications, deletions, and sequence modifications of existing proteins. indirect information about relative rates and other intrinsic parameters of these three basic processes is contained in the proteome-wide distribution of sequence identities of pairs of paralogous proteins. | 2007 | 18039386 |
| an acetylation/deacetylation cycle controls the export of sterols and steroids from s. cerevisiae. | sterol homeostasis in eukaryotic cells relies on the reciprocal interconversion of free sterols and steryl esters. here we report the identification of a novel reversible sterol modification in yeast, the sterol acetylation/deacetylation cycle. sterol acetylation requires the acetyltransferase atf2, whereas deacetylation requires say1, a membrane-anchored deacetylase with a putative active site in the er lumen. lack of say1 results in the secretion of acetylated sterols into the culture medium, ... | 2007 | 18034159 |
| interaction between transcription factor, basal transcription factor 3, and the nh2-terminal domain of human estrogen receptor alpha. | the estrogen receptor (er), like other members of the nuclear receptor superfamily, possesses two separate transcriptional activation functions, af-1 and af-2. although a variety of coactivators and corepressors of af-2 have been identified, less is known of the mechanism of action of af-1. we have used the yeast two-hybrid system to isolate a cdna coding for a protein that binds specifically to the af-1 region of human eralpha. this cdna codes for the transcription factor basal transcription fa ... | 2007 | 18025262 |
| mftz-1, an actinomycetes subspecies derived antitumor macrolide, functions as a novel topoisomerase ii poison. | 14-ethyl-2,5,11-trimethyl-4,13,19,20-tetraoxa-tricyclo[14.2.1.1(7,10)]eicosane-3,12-dione (mftz-1), a new macrolide compound isolated from streptomyces sp. is9131, displayed wide cytotoxicity in human tumor cell lines with an average ic(50) of 0.905 micromol/l. notably, mftz-1 showed significant cytotoxicity in the three multidrug resistance cell lines with an average resistance factor of 2.08. the in vivo experiments showed that mftz-1 had inhibitory effects on the human ovarian carcinoma ho-89 ... | 2007 | 18025289 |
| purifying the hedgehog protein and its variants. | the purification of recombinant versions of the n-terminal signaling fragment of sonic hedgehog (shhn) from e. coli, hi-5 insect cells, yeast, and mammalian cell sources reveals diverse post-translational modifications that affect the potency of the purified protein. modifications to the n-terminal cysteine with fatty acyl groups results in significant increases in potency, up to 100-fold, when compared with the unmodified protein. proteolytic clipping at sites near the n-terminus results in ina ... | 2007 | 18025709 |
| inhibition of the pathogenically related morphologic transition in candida albicans by disrupting cdc42 binding to its effectors. | morphologic transition from the yeast to the hyphal state in candida albicans is associated with pathogenicity of this human pathogen. such invasive transition of c. albicans cells is regulated by numerous cell signal transduction pathways, one of which involves a small gtpase, the c. albicans cdc42 (cacdcd42), with specific binding to downstream effectors, e.g., cacla4 and cst20, containing crib domains. here, we report that in vivo inhibition of cacdc42 by peptide-mediated transduction of the ... | 2007 | 18022566 |
| expression and physiological function of cyp4f subfamily in human eosinophils. | we investigated expression of the cyp4f subfamily in human leukocytes by flow cytometry using anti-cyp4f3a antibody and quantitative reverse transcription-polymerase chain reaction (qrt-pcr). more than 90% of cd11b, cd13, cd14, cd33, and eosinophil marker-positive cells expressed cyp4f3a. mrna for cyp4f3a was found in neutrophils, monocytes, and eosinophils. cyp4f12 mrna was detected in eosinophils and neutrophils. in eosinophils, transcription of the cyp4f12 gene was started from two sites at 4 ... | 2007 | 17980168 |
| a human na+/h+ antiporter sharing evolutionary origins with bacterial nhaa may be a candidate gene for essential hypertension. | phylogenetic analysis of the cation/proton antiporter superfamily has uncovered a previously unknown clade of genes in metazoan genomes, including two previously uncharacterized human isoforms, nha1 and nha2, found in tandem on human chromosome 4. the nha (sodium hydrogen antiporter) family members share significant sequence similarity with escherichia coli nhaa, including a conserved double aspartate motif in predicted transmembrane 5. we show that hsnha2 (homo sapiens nha2) resides on the plas ... | 2007 | 18000046 |
| two closely related recq helicases have antagonistic roles in homologous recombination and dna repair in arabidopsis thaliana. | recq helicases are involved in the processing of dna structures arising during replication, recombination, and repair throughout all kingdoms of life. mutations of different recq homologues are responsible for severe human diseases, such as blooms (blm) or werner (wrn) syndrome. the loss of recq function is often accompanied by hyperrecombination caused by a lack of crossover suppression. in the arabidopsis genome seven different recq genes are present. two of them (atrecq4a and 4b) arose becaus ... | 2007 | 18000056 |
| a conserved dna damage response pathway responsible for coupling the cell division cycle to the circadian and metabolic cycles. | the circadian clock drives endogenous oscillations of cellular and physiological processes with a periodicity of approximately 24 h. progression of the cell division cycle (cdc) has been found to be coupled to the circadian clock, and it has been postulated that gating of the cdc by the circadian cycle may have evolved to protect dna from the mutagenic effects of ultraviolet light. when grown under nutrient-limiting conditions in a chemostat, prototrophic strains of budding yeast, saccharomyces ... | 2007 | 18000400 |
| the kelch protein ns1-bp interacts with alpha-enolase/mbp-1 and is involved in c-myc gene transcriptional control. | alpha-enolase is a key glycolytic enzyme that plays a functional role in several physiological processes depending on the cellular localization. the enzyme is mainly localized in the cytoplasm whereas an alternative translated form, named mbp-1, is predominantly nuclear. the mbp-1 protein has been characterized as a c-myc promoter binding protein that negatively controls transcription. in the present study, we identified the kelch protein ns1-bp as one of the alpha-enolase/mbp-1 partners by usin ... | 2007 | 17996313 |
| the arabidopsis rna-binding protein fca requires a lysine-specific demethylase 1 homolog to downregulate flc. | a repressor of the transition to flowering in arabidopsis is the mads box protein flowering locus c (flc). fca, an rna-binding protein, and fy, a homolog of the yeast rna 3' processing factor pfs2p, downregulate flc expression and therefore promote flowering. fca/fy physically interact and alter polyadenylation/3' processing to negatively autoregulate fca. here, we show that fca requires flowering locus d (fld), a homolog of the human lysine-specific demethylase 1 (lsd1) for flc downregulation. ... | 2007 | 17996704 |
| comparative analyses of complex formation and binding sites between human tumor necrosis factor-alpha and its three antagonists elucidate their different neutralizing mechanisms. | tumor necrosis factor-alpha (tnfalpha)-blocking therapy, using biologic tnfalpha antagonists, has been approved for the treatment of several diseases including rheumatoid arthritis, psoriasis and crohn's disease. there have been few detailed studies of binding characterizations for the complex formation by tnfalpha and clinically relevant antagonists, particularly infliximab (remicade) and etanercept (enbrel). here we characterized the binding stoichiometry and size of soluble tnfalpha-antagonis ... | 2007 | 17996896 |
| a p21-activated kinase is required for conidial germination in penicillium marneffei. | asexual spores (conidia) are the infectious propagules of many pathogenic fungi, and the capacity to sense the host environment and trigger conidial germination is a key pathogenicity determinant. germination of conidia requires the de novo establishment of a polarised growth axis and consequent germ tube extension. the molecular mechanisms that control polarisation during germination are poorly understood. in the dimorphic human pathogenic fungus penicillium marneffei, conidia germinate to prod ... | 2007 | 17983267 |
| crystal structure of human daam1 formin homology 2 domain. | reorganization of the actin filament is an essential process for cell motility, cell-cell attachment and intracellular transport. formin proteins promote nucleation and elongation of the actin filament, and thus are key regulators for this process. the formin homology 2 (fh2) domain forms a head-to-tail ring-shaped dimer, and processively moves towards the barbed end. dishevelled-associated activator of morphogenesis (daam) is a rho-regulated formin implicated in neuronal development. here, we p ... | 2007 | 17986009 |
| the ddx3 subfamily of the dead box helicases: divergent roles as unveiled by studying different organisms and in vitro assays. | ddx3 (or ded1p), the highly conserved subfamily of the dead-box rna helicase family (40 members in humans), plays important roles in rna metabolism. ddx3x and ddx3y, the two human paralogous genes of this subfamily of proteins, have orthologous candidates in a diverse range of eukaryotes, from yeast and plants to animals. while ddx3y, which is essential for normal spermatogenesis, is translated only in the testes, ddx3x protein is ubiquitously expressed, involved in rna transcription, rna splici ... | 2007 | 17979704 |
| systems analysis of chaperone networks in the malarial parasite plasmodium falciparum. | molecular chaperones participate in the maintenance of cellular protein homeostasis, cell growth and differentiation, signal transduction, and development. although a vast body of information is available regarding individual chaperones, few studies have attempted a systems level analysis of chaperone function. in this paper, we have constructed a chaperone interaction network for the malarial parasite, plasmodium falciparum. p. falciparum is responsible for several million deaths every year, an ... | 2007 | 17941702 |
| genetic determinants of mitochondrial response to arsenic in yeast saccharomyces cerevisiae. | we have used yeast saccharomyces cerevisiae as a tool to identify the importance of mitochondrial processes involved in arsenic-induced carcinogenicity in humans. we screened 466 single-gene knockout strains of yeast s. cerevisiae known to be involved in biogenesis of mitochondria for sodium arsenite (asiii) and sodium arsenate (asv) sensitivity. we identified 72 arsenite-sensitive and 81 arsenate-sensitive mutants. we categorized the identified mutants based on the various mitochondrial process ... | 2007 | 17942904 |
| cr/periphilin is a transcriptional co-repressor involved in cell cycle progression. | cr/periphilin (cr) retards cell cycle progression mainly at the s-phase in part by transcriptionally repressing expression of cdc7, the key regulator of dna replication, and in part by unknown mechanisms. in this study, we show that enforced expression of cr inhibits cdc7 promoter activity. the attachment of the dna-binding domain of the yeast gal4 transcription factor to cr that appears without dna-binding sequences, enables cr to repress gal4 promoter-mediated transcription in a histone deacet ... | 2007 | 17963697 |
| human ctip promotes dna end resection. | in the s and g2 phases of the cell cycle, dna double-strand breaks (dsbs) are processed into single-stranded dna, triggering atr-dependent checkpoint signalling and dsb repair by homologous recombination. previous work has implicated the mre11 complex in such dsb-processing events. here, we show that the human ctip (rbbp8) protein confers resistance to dsb-inducing agents and is recruited to dsbs exclusively in the s and g2 cell-cycle phases. moreover, we reveal that ctip is required for dsb res ... | 2007 | 17965729 |
| effect of dietary selenium and omega-3 fatty acids on muscle composition and quality in broilers. | human health may be improved if dietary intakes of selenium and omega-3 fatty acids are increased. consumption of broiler meat is increasing, and the meat content of selenium and omega-3 fatty acids are affected by the composition of broiler feed. a two-way analyses of variance was used to study the effect of feed containing omega-3 rich plant oils and selenium enriched yeast on broiler meat composition, antioxidation- and sensory parameters. four different wheat-based dietary treatments supplem ... | 2007 | 17967172 |
| current prospects for the production of coenzyme q10 in microbes. | coenzyme q or ubiquinone (uq) is a naturally occurring coenzyme formed from the conjugation of a benzoquinone ring and an isoprenoid chain of varying length. uq-10, the main uq species produced by humans, provides therapeutic benefits in certain human diseases, such as cardiomyopathy, when administered orally. increased consumer demand has led to the development of bioprocesses for the commercial production of uq-10. up to now, these processes have relied on microbes that produce high levels of ... | 2007 | 17935805 |
| hd-ptp and alix share some membrane-traffic related proteins that interact with their bro1 domains or proline-rich regions. | mammalian alix is a multifunctional adaptor protein involved in cell death, receptor endocytosis, endosomal protein sorting and cell adhesion by associating with various proteins such as alg-2, cin85/rukl/seta, endophilins, chmp4s and tsg101. hd-ptp is a paralog of alix and a putative protein tyrosine phosphatase (ptp) that contains a bro1 domain, coiled-coils, a proline-rich region (prr) in addition to a ptp domain. we investigated interactions between hd-ptp and alix-binding proteins. in the y ... | 2007 | 17174262 |
| protein tyrosine phosphatase h1 is a target of the e6 oncoprotein of high-risk genital human papillomaviruses. | the e6 proteins of high-risk genital human papillomaviruses (hpv), such as hpv types 16 and 18, possess a conserved c-terminal pdz-binding motif, which mediates interaction with some cellular pdz domain proteins. the binding of e6 usually results in their ubiquitin-mediated degradation. the ability of e6 to bind to pdz domain proteins correlates with the oncogenic potential. using a yeast two-hybrid system, gst pull-down experiments and coimmunoprecipitations, we identified the protein tyrosine ... | 2007 | 17947517 |
| structural characterization of the atpase reaction cycle of endosomal aaa protein vps4. | the multivesicular body (mvb) pathway functions in multiple cellular processes including cell surface receptor down-regulation and viral budding from host cells. an important step in the mvb pathway is the correct sorting of cargo molecules, which requires the assembly and disassembly of endosomal sorting complexes required for transport (escrts) on the endosomal membrane. disassembly of the escrts is catalyzed by atpase associated with various cellular activities (aaa) protein vps4. vps4 contai ... | 2007 | 17949747 |
| biochemical analysis of inositol phosphate kinases. | lipid-derived inositol phosphates (ips) are a complex group of second messengers generated by the sequential phosphorylation of inositol 1,4,5-trisphosphate (ip(3)). synthetic pathways leading from ip(3) to the formation of inositol tetrakisphosphate ip(4), inositol pentakisphosphate ip(5), inositol hexakisphosphate ip(6), and inositol pyrophosphates pp-ips have been elucidated in eukaryotes from yeast to human. studies have attributed a variety of cellular functions to ips, highlighting the imp ... | 2007 | 17954248 |
| overaccumulation of higher polyamines in ripening transgenic tomato fruit revives metabolic memory, upregulates anabolism-related genes, and positively impacts nutritional quality. | vegetables and fruits are essential components of the human diet as they are sources of vitamins, minerals, and fiber and provide antioxidants that prevent chronic diseases. our goal is to improve durable nutritional quality of tomato fruit. we developed transgenic tomatoes expressing yeast s-adenosylmethionine decarboxylase (ysamdc) gene driven by a fruit-specific e8 promoter to investigate the role of polyamines in fruit metabolism. stable integration of e8-ysamdc chimeric gene in tomato genom ... | 2007 | 17955994 |
| suppression of polyglutamine toxicity by the yeast sup35 prion domain in drosophila. | the propensity of proteins to form beta-sheet-rich amyloid fibrils is related to a variety of biological phenomena, including a number of human neurodegenerative diseases and prions. a subset of amyloidogenic proteins forms amyloid fibrils through glutamine/asparagine (q/n)-rich domains, such as pathogenic polyglutamine (poly(q)) proteins involved in neurodegenerative disease, as well as yeast prions. in the former, the propensity of an expanded poly(q) tract to abnormally fold confers toxicity ... | 2007 | 17956866 |
| regulation of mitochondrial fusion and division. | in many organisms, ranging from yeast to humans, mitochondria fuse and divide to change their morphology in response to a multitude of signals. during the past decade, work using yeast and mammalian cells has identified much of the machinery required for fusion and division, including the dynamin-related gtpases--mitofusins (fzo1p in yeast) and opa1 (mgm1p in yeast) for fusion and drp1 (dnm1p) for division. however, the mechanisms by which cells regulate these dynamic processes have remained lar ... | 2007 | 17959383 |
| gpm1p is a factor h-, fhl-1-, and plasminogen-binding surface protein of candida albicans. | the human pathogenic yeast candida albicans utilizes host complement regulators for immune evasion. here we identify the first fungal protein that binds factor h and fhl-1. by screening a protein array of 4088 proteins of saccharomyces cerevisiae, phosphoglycerate mutase (scgpm1p) was identified as a factor h- and fhl-1-binding protein. the homologous c. albicans gpm1p (cagpm1p) was cloned and recombinantly expressed as a 36-kda his-tagged protein. purified cagpm1p binds the host complement regu ... | 2007 | 17959597 |
| altered, but not diminished specific t cell response in chronic mucocutaneous candidiasis patients. | patients suffering from chronic mucocutaneous infections with the yeast candida albicans (cmc) are discussed to have an underlying primary cellular immunodeficiency. in order to characterise cellular immunity in cmc patients, we analysed chemotaxis and myeloperoxidase (mpo) releases of neutrophils and t cell proliferation and cytokine production to candida albicans. patients with chronic mucocutaneous candidiasis (n = 4) and healthy volunteers of same sex and similar age (n = 14) were enrolled i ... | 2007 | 17960405 |
| studies on fungal pumilio gene family through mining multiple genome-scale data sets. | the genes belonging to pumilio gene family of the fission yeast schizosaccharomyces pombe (s. pombe) were compared with genome information of the several fungi and their functions have been inferred using public-access databases disclosed on the internet websites. the pumilio-family genes are conserved from yeast to man and have been considered to suppress the expression of other genes by binding their specific target messenger rnas. in s. pombe genome, nine genes belonging to pumilio gene famil ... | 2007 | 17932456 |
| bioactivity and stability analysis of endostatin purified from fermentation supernatant of 293 cells transfected with ad/rhendo. | endostatin is a potent angiogenic inhibitor that has been approved for the treatment of cancer. however, endostatin is unstable in vitro and difficult to produce in large quantities. endostatin gene therapy is an alternative to overcome these difficulties by expressing sustained bioactive endostatin in vivo. we previously developed a recombinant replication-defective adenovirus, e10a, which carries the human endostatin gene. phase i trial of e10a given as a weekly intratumoral injection in adult ... | 2007 | 17933553 |
| high rate of starvation-associated mutagenesis in ung(-) yeast caused by the overproduction of human activation-induced deaminase. | we examined the role of saccharomyces cerevisiae uracil dna glycosylase in the suppression of mutagenesis in non-dividing, adenine-starved cells expressing human activation-induced deaminase (aid) gene. our aim was to further understand the mechanisms preventing starvation-associated mutagenesis in yeast and to explore the consequences of aid gene expression in non-proliferating eukaryotic cells. genetic control of starvation-induced mutagenesis in many aspects is similar to the control of spont ... | 2007 | 17934734 |
| protein transduction as a means of effective manipulation of cdc42 activity in primary t cells. | the rho family gtpase cdc42 is a critical regulator of cellular polarization from yeast to man. an analysis of its function in t cell activation is therefore of interest. this analysis poses two substantial challenges, similar to the analysis of many other critical t cell signaling intermediates. first, cdc42 is required for development and cell survival, necessitating short-term manipulation of its activity. second, cdc42 is likely involved in multiple signaling pathways, requiring approaches t ... | 2007 | 17188290 |
| expression, purification, and characterization of secreted recombinant human insulin-like growth factor-binding protein-6 in methylotrophic yeast pichia pastoris. | the mitogenic and metabolic activities of insulin-like growth factors (igf) are modulated by a family of six high-affinity igf-binding proteins (igfbps). this study describes the secretion and purification of the recombinant human igfbp-6 expressed in methylotrophic yeast pichia pastoris. in this research, a multicopy expression plasmid pa-o815/3xigfbp-6 containing 3 copies of human igfbp-6 expression cassette was constructed and transformed into p. pastoris gs115. the encoding sequence of alpha ... | 2007 | 17188511 |
| the novel cellular mechanism of human 5-ht6 receptor through an interaction with fyn. | the human 5-ht(6) receptor (5-ht(6)r) is one of the latest cloned receptors among the known 5-ht receptors. its abundant distribution in the limbic region, which participates in the control of mood and emotion and is involved in nervous system diseases such as depression and alzheimer disease, has caused it to generate much interest. however, the cellular mechanisms of 5-ht(6)r are poorly understood. in the present study we found, using a yeast two-hybrid assay, that the carboxyl-terminal region ... | 2007 | 17189269 |
| atomic model of human rcd-1 reveals an armadillo-like-repeat protein with in vitro nucleic acid binding properties. | rcd-1, a protein highly conserved across eukaryotes, was initially identified as a factor essential for nitrogen starvation-invoked differentiation in fission yeast, and its saccharomyces cerevisiae homolog, caf40, has been identified as part of the ccr4-not transcription complex, where it interacts with the not1 protein. mammalian homologs are involved in various cellular differentiation processes including retinoic acid-induced differentiation and hematopoetic cell development. here, we presen ... | 2007 | 17189474 |
| sharcgs, a fast and highly accurate short-read assembly algorithm for de novo genomic sequencing. | the latest revolution in the dna sequencing field has been brought about by the development of automated sequencers that are capable of generating giga base pair data sets quickly and at low cost. applications of such technologies seem to be limited to resequencing and transcript discovery, due to the shortness of the generated reads. in order to extend the fields of application to de novo sequencing, we developed the sharcgs algorithm to assemble short-read (25-40-mer) data with high accuracy a ... | 2007 | 17908823 |
| the ephd protein spbp interacts with topbp1 and together they co-operate to stimulate ets1-mediated transcription. | spbp (stromelysin-1 pdgf responsive element binding protein) is a ubiquitously expressed 220 kda nuclear protein shown to enhance or repress the transcriptional activity of various transcription factors. a yeast two-hybrid screen, with the extended plant homeodomain (ephd) of spbp as bait, identified topbp1 (topoisomerase ii beta-binding protein 1) as a candidate interaction partner of spbp. topbp1 has eight brca1 carboxy-terminal (brct) domains and is involved in dna replication, dna damage res ... | 2007 | 17913746 |
| dominant-negative behavior of mammalian vps35 in yeast requires a conserved prlyl motif involved in retromer assembly. | the retromer protein complex assists in recycling selected integral membrane proteins from endosomes to the trans golgi network. one protein subcomplex (vps35p, vps26p and vps29p) combines with a second (vps17p and vps5p) to form a coat involved in sorting and budding of endosomal vesicles. yeast vps35p (yvps35) exhibits similarity to human vps35 (hvps35), especially in a completely conserved prlyl motif contained within an amino-terminal domain. companion studies indicate that an r(98)w mutatio ... | 2007 | 17916227 |
| genes2networks: connecting lists of gene symbols using mammalian protein interactions databases. | in recent years, mammalian protein-protein interaction network databases have been developed. the interactions in these databases are either extracted manually from low-throughput experimental biomedical research literature, extracted automatically from literature using techniques such as natural language processing (nlp), generated experimentally using high-throughput methods such as yeast-2-hybrid screens, or interactions are predicted using an assortment of computational approaches. genes or ... | 2007 | 17916244 |
| caf-1 is essential for drosophila development and involved in the maintenance of epigenetic memory. | dna synthesis during s-phase and upon dna repair is accompanied by chromatin assembly. the chromatin assembly factor caf-1 has been biochemically well-characterized to deposit histones onto newly synthesized dna. to gain insights into the in vivo functions of caf-1 in drosophila, we generated null mutants of the largest subunit of dcaf-1, dcaf-1-p180. we show that, unlike caf-1 mutant yeast, dcaf-1-p180 mutant flies are hemizygous lethal. removal of maternal dcaf-1-p180 activity by germline clon ... | 2007 | 17916346 |
| functional expression and characterisation of a gut facilitative glucose transporter, nlht1, from the phloem-feeding insect nilaparvata lugens (rice brown planthopper). | phloem-sap feeding hemipteran insects have access to a sucrose-rich diet but are dependent on sucrose hydrolysis and hexose transport for carbon nutrition. a cdna library from nilaparvata lugens (rice brown planthopper) was screened for clones encoding potential transmembrane transporters. a selected cdna, nlht1, encodes a 53kda polypeptide with sequence similarity to facilitative hexose transporters of eukaryotes and prokaryotes, including glut1, the human erythrocyte hexose transporter. nlht1 ... | 2007 | 17916500 |
| allosteric loss-of-function mutations in hiv-1 nef from a long-term non-progressor. | activation of src family kinases by human immunodeficiency virus type 1 (hiv-1) nef may play an important role in the pathogenesis of hiv/aids. here we investigated whether diverse nef sequences universally activate hck, a src family member expressed in macrophages and other hiv-1 target cells. in general, we observed that hck activation is a highly conserved nef function. however, we identified an unusual nef variant from an hiv-positive individual that did not develop aids which failed to acti ... | 2007 | 17920628 |
| pathobiology of fungal infections of the central nervous system with special reference to the indian scenario. | ubiquitously present fungi in the environment find a nidus in the human body and adopt its metabolic machinery to be in symbiosis or become pathogenic. immunocompromised states like human immunodeficiency virus (hiv) / acquired immunodeficiency syndrome (aids), systemic neoplasia and organ transplantation have enhanced the frequency of fungal infections. high-risk behavior, iv drug abuse and air travel have led to the emergence of new fungal infections hitherto geographically localized. the path ... | 2007 | 17921648 |
| rna analysis by mekc with lif detection. | we have developed and validated a procedure of high sensitivity for the analysis of rna. the procedure is based on the separation and detection of the 5'-monophosphates of ribonucleosides selectively conjugated with 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl ethylene diamine hydrochloride (bodipy fl eda) at the 5'-phosphate group using ce with lif. bodipy conjugates of the four common ribonucleoside-5'-monophosphates were prepared and subjected to ce-lif to serve as stan ... | 2007 | 17922502 |
| a novel dominant mutation in plakoglobin causes arrhythmogenic right ventricular cardiomyopathy. | arrhythmogenic right ventricular cardiomyopathy (arvc) is an inherited disorder associated with arrhythmias and sudden death. a recessive mutation in the gene encoding plakoglobin has been shown to cause naxos disease, a cardiocutaneous syndrome characterized by arvc and abnormalities of hair and skin. here, we report, for the first time, a dominant mutation in the gene encoding plakoglobin in a german family with arvc but no cutaneous abnormalities. the mutation (s39_k40inss) is predicted to in ... | 2007 | 17924338 |
| structural and functional insights into dom34, a key component of no-go mrna decay. | the yeast protein dom34 is a key component of no-go decay, by which mrnas with translational stalls are endonucleolytically cleaved and subsequently degraded. however, the identity of the endoribonuclease is unknown. homologs of dom34, called pelota, are broadly conserved in eukaryotes and archaea. to gain insights into the structure and function of dom34/pelota, we have determined the structure of pelota from thermoplasma acidophilum (ta pelota) and investigated the ribonuclease activity of dom ... | 2007 | 17889667 |
| sel1 repeat protein lpne is a legionella pneumophila virulence determinant that influences vacuolar trafficking. | the environmental pathogen legionella pneumophila possesses five proteins with sel1 repeats (slrs) from the tetratricopeptide repeat protein family. three of these proteins, lpne, enhc, and lidl, have been implicated in the ability of l. pneumophila to efficiently establish infection and/or manipulate host cell trafficking events. previously, we showed that lpne is important for l. pneumophila entry into macrophages and epithelial cells. in further virulence studies here, we show that lpne is al ... | 2007 | 17893138 |
| recycling of sphingosine is regulated by the concerted actions of sphingosine-1-phosphate phosphohydrolase 1 and sphingosine kinase 2. | in yeast, the long-chain sphingoid base phosphate phosphohydrolase lcb3p is required for efficient ceramide synthesis from exogenous sphingoid bases. similarly, in this study, we found that incorporation of exogenous sphingosine into ceramide in mammalian cells was regulated by the homologue of lcb3p, sphingosine-1-phosphate phosphohydrolase 1 (spp-1), an endoplasmic reticulum resident protein. sphingosine incorporation into endogenous long-chain ceramides was increased by spp-1 overexpression, ... | 2007 | 17895250 |
| signaling of human frizzled receptors to the mating pathway in yeast. | frizzled receptors have seven membrane-spanning helices and are considered as atypical g protein-coupled receptors (gpcrs). the mating response of the yeast saccharomyces cerevisiae is mediated by a gpcr signaling system and this model organism has been used extensively in the past to study mammalian gpcr function. we show here that human frizzled receptors (fz1 and fz2) can be properly targeted to the yeast plasma membrane, and that they stimulate the yeast mating pathway in the absence of adde ... | 2007 | 17895994 |
| mvb-12, a fourth subunit of metazoan escrt-i, functions in receptor downregulation. | after ligand binding and endocytosis, cell surface receptors can continue to signal from endosomal compartments until sequestered from the cytoplasm. an important mechanism for receptor downregulation in vivo is via the inward budding of receptors into intralumenal vesicles to form specialized endosomes called multivesicular bodies (mvbs) that subsequently fuse with lysosomes, degrading their cargo. this process requires four heterooligomeric protein complexes collectively termed the escrt machi ... | 2007 | 17895996 |
| different contribution of co-stimulatory molecules b7.1 and b7.2 to the immune response to recombinant modified vaccinia virus ankara vaccine expressing prm/e proteins of japanese encephalitis virus and two hepatitis b virus vaccines. | this study clarifies the role of co-stimulatory molecules b7.1 and b7.2 in the immune response to 3 types of vaccines: a/ recombinant modified vaccinia virus ankara (mva) (vjh9) expressing prm/e proteins of japanese encephalitis virus (jev), b/ recombinant yeast-expressed hepatitis b virus (yhbv), c/ human plasma-derived hepatitis b virus (phbv). we constructed plasmids expressing b7.1 and b7.2 molecules and found that the expression level of b7.2 protein in transfected cho-k1 cells was higher t ... | 2007 | 17900219 |
| roles of the negatively charged n-terminal extension of saccharomyces cerevisiae ribosomal protein s5 revealed by characterization of a yeast strain containing human ribosomal protein s5. | ribosomal protein (rp) s5 belongs to a family of ribosomal proteins that includes bacterial rps7. rps5 forms part of the exit (e) site on the 40s ribosomal subunit and is essential for yeast viability. human rps5 is 67% identical and 79% similar to saccharomyces cerevisiae rps5 but lacks a negatively charged (pi approximately 3.27) 21 amino acid long n-terminal extension that is present in fungi. here we report that replacement of yeast rps5 with its human homolog yielded a viable yeast strain w ... | 2007 | 17901157 |
| the yeast connection: is candida linked to breastfeeding associated pain? | the objective of the study was to determine whether the isolation of candida from breastfeeding women is associated with self-reported pain. | 2007 | 17904988 |
| o-mannosyltransferase 1 in aspergillus fumigatus (afpmt1p) is crucial for cell wall integrity and conidium morphology, especially at an elevated temperature. | protein o-mannosyltransferases initiate o mannosylation of secretory proteins, which are of fundamental importance in eukaryotes. in this study, the pmt gene family of the human fungal pathogen aspergillus fumigatus was identified and characterized. unlike the case in saccharomyces cerevisiae, where the pmt family is highly redundant, only one member of each pmt subfamily, namely, afpmt1, afpmt2, and afpmt4, is present in a. fumigatus. mutants with a deletion of afpmt1 are viable. in vitro and i ... | 2007 | 17905922 |
| structural studies of the final enzyme in the alpha-aminoadipate pathway-saccharopine dehydrogenase from saccharomyces cerevisiae. | the 1.64 a structure of the apoenzyme form of saccharopine dehydrogenase (sdh) from saccharomyces cerevisiae shows the enzyme to be composed of two domains with similar dinucleotide binding folds with a deep cleft at the interface. the structure reveals homology to alanine dehydrogenase, despite low primary sequence similarity. a model of the ternary complex of sdh, nad, and saccharopine identifies residues lys77 and glu122 as potentially important for substrate binding and/or catalysis, consist ... | 2007 | 17854830 |
| pulmonary histoplasmosis as an example of imported mycoses in japan. | as travel increases, histoplasmosis as an imported mycosis that has the potential to increase abruptly in japan. a 56-year-old japanese man who had been in bolivia for 2 years complained of a dry cough. chest computed tomography revealed multiple lesions in the right and left lower lobes of his lung that were difficult to diagnose and differentiate from advanced lung cancer. we performed surgical resection for biopsy by video-assisted thoracoscopic surgery. an intraoperative histopathological ex ... | 2007 | 17867281 |
| involvement of rela-associated inhibitor in regulation of trophoblast differentiation via interaction with transcriptional factor specificity protein-1. | glucose transporter-1 (glut1), one of the key functional indicators of placental differentiation, has an important role in placental glucose transport. we previously showed that the protein levels of glut1 and nuclear transcription factor specificity protein-1 (sp1) in rat choriocarcinoma cells (rcho-1 cells) decreased during the differentiation of these cells to giant cells. we also showed that sp1 was involved in the regulation of glut1 gene expression during this process. rela-associated inhi ... | 2007 | 17872376 |
| novel membrane-bound eif2alpha kinase in the flagellar pocket of trypanosoma brucei. | translational control mediated by phosphorylation of the alpha subunit of the eukaryotic initiation factor 2 (eif2alpha) is central to stress-induced programs of gene expression. trypanosomatids, important human pathogens, display differentiation processes elicited by contact with the distinct physiological milieu found in their insect vectors and mammalian hosts, likely representing stress situations. trypanosoma brucei, the agent of african trypanosomiasis, encodes three potential eif2alpha ki ... | 2007 | 17873083 |
| nucleolar translocalization of gra10 of toxoplasma gondii transfectionally expressed in hela cells. | toxoplasma gondii gra10 expressed as a gfp-gra10 fusion protein in hela cells moved to the nucleoli within the nucleus rapidly and entirely. gra10 was concentrated specifically in the dense fibrillar component of the nucleolus morphologically by the overlap of gfp-gra10 transfection image with ifa images by monoclonal antibodies against gra10 (tg378), b23 (nucleophosmin) and c23 (nucleolin). the nucleolar translocalization of gra10 was caused by a putative nucleolar localizing sequence (nols) of ... | 2007 | 17876161 |
| tpr-mediated self-association of plant sgt1. | the tetratricopeptide repeat (tpr) domain mediates inter-protein associations in a number of systems. the domain is also thought to mediate oligomerization of some proteins, but this has remained controversial, with conflicting data appearing in the literature. by way of investigating such tpr-mediated self-associations we used a variety of biophysical techniques to characterize purified recombinant sgt1, a tpr-containing protein found in all eukaryotes that is involved in a broad range of biolo ... | 2007 | 17877371 |
| hemocompatibility of hydrophilic antimicrobial copolymers of alkylated 4-vinylpyridine. | quaternized poly(vinylpyridine) (pvp) is a polymer with inherent antimicrobial properties that is effective against gram-positive bacteria, gram-negative bacteria, viruses, and yeast cells. however, quaternized pvp has poor biocompatibility, which prevents its use in biomaterial applications. copolymerization was examined as a method of modifying the structure to incorporate biocompatibility. polyethyleneglycol methyl ether methacrylate (pegma) and hydroxyethyl methacrylate (hema) are polymers g ... | 2007 | 17877398 |
| interactions between subunits of saccharomyces cerevisiae rnase mrp support a conserved eukaryotic rnase p/mrp architecture. | ribonuclease mrp is an endonuclease, related to rnase p, which functions in eukaryotic pre-rrna processing. in saccharomyces cerevisiae, rnase mrp comprises an rna subunit and ten proteins. to improve our understanding of subunit roles and enzyme architecture, we have examined protein-protein and protein-rna interactions in vitro, complementing existing yeast two-hybrid data. in total, 31 direct protein-protein interactions were identified, each protein interacting with at least three others. fu ... | 2007 | 17881380 |
| shy1 couples cox1 translational regulation to cytochrome c oxidase assembly. | cytochrome c oxidase (complex iv) of the respiratory chain is assembled from nuclear and mitochondrially-encoded subunits. defects in the assembly process lead to severe human disorders such as leigh syndrome. shy1 is an assembly factor for complex iv in saccharomyces cerevisiae and mutations of its human homolog, surf1, are the most frequent cause for leigh syndrome. we report that shy1 promotes complex iv biogenesis through association with different protein modules; shy1 interacts with mss51 ... | 2007 | 17882259 |
| expression, purification, and characterization of recombinant human keratinocyte growth factor-2 in pichia pastoris. | keratinocyte growth factor-2 (kgf-2) is a member of the fibroblast growth factor family. the full-length human kgf-2 coding sequence, gained by synthesizing, was cloned into the ppiczalphaa vector in frame with the yeast alpha-factor secretion signal under the transcriptional control of the aox promoter and integrated into pichia pastoris strain gs115. in shake-flask culture induced with methanol, the rhkgf-2 content was about 17.5% of the total secreted proteins. under the optimal conditions, s ... | 2007 | 17884221 |
| composition, enantiomeric distribution, and antimicrobial activity of tanacetum argenteum subsp. flabellifolium essential oil. | tanacetum argenteum (lam.) willd. subsp. flabellifolium (boiss. & heldr.) grierson of asteraceae is an endemic species in turkey. hydrodistillation of aerial parts using a clevenger apparatus yielded an essential oil, which was subsequently analyzed by gas chromatography-mass spectroscopy (gc-ms). alpha-pinene (29%), (e)-sesquilavandulol (16%), and camphor (14%) were found as main constituents. enantiomeric distribution of the monoterpenes alpha-pinene and camphor was determined on a fused silic ... | 2007 | 17884324 |
| some highlights of research on aging with invertebrates, 2006-2007. | the invertebrate model organisms continue to be engines of discovery in aging research. recent work with drosophila stem cells has thrown light on their human equivalents, and on the role of stem cells and their niches in the decline in fecundity with age. inspired by observations of aging in bacteria and yeast, a new theoretical study has revealed evolutionary forces that could favour asymmetry in the distribution of damaged cell constituents at division, and hence pave the way for the evolutio ... | 2007 | 17725687 |
| the dna polymerase gamma y955c disease variant associated with peo and parkinsonism mediates the incorporation and translesion synthesis opposite 7,8-dihydro-8-oxo-2'-deoxyguanosine. | mitochondrial dna is replicated and repaired by dna polymerase gamma (pol gamma), encoded by the polg gene. the y955c substitution in polg leads to autosomal dominant progressive external ophthalmoplegia (peo) with other severe phenotypes. peo patients with this mutation can further develop parkinsonism or premature ovarian failure. mouse and yeast models with this mutation show enhanced amounts of oxidative lesions and increased mtdna damage. in dna pol gamma, tyr955 plays a critical role in ca ... | 2007 | 17725985 |