Publications

chemoenzymatic synthesis of feruloyl d-arabinose as a potential anti-mycobacterial agent.	the feruloyl esterase (stfaec) produced by sporotrichum thermophile transfered the feruloyl group to d: -arabinose using a mixture of n-hexane, t-butanol and water. about 45% conversion of d: -arabinose to the feruloylated derivative was achieved. the compound had an mic value against mycobacterium bovis bcg of 25 microg/ml.	2007	17676274
novel cephalosporins synthesized by amination of 2,5-dihydroxybenzoic acid derivatives using fungal laccases ii.	sixteen novel cephalosporins were synthesized by amination of 2,5-dihydroxybenzoic acid derivatives with the aminocephalosporins cefadroxil, cefalexin, cefaclor, and the structurally related carbacephem loracarbef using laccases from trametes sp. or myceliophthora thermophila. all products inhibited the growth of several gram positive bacterial strains in the agar diffusion assay, among them methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococci. the products protected ...	2007	17329882
antioxidants determination with laccase.	the spectrophotometric method of antioxidants determination using recombinant laccase polyporus pinsitus (rppl) and myceliophthora thermophila (rmtl) was developed. the method includes simultaneous oxidation of the antioxidant and high reactive laccase substrate producing chromophoric radical cation. as laccase substrates abts and other high reactive phenoxazine derivatives: 2-phenoxazin-10-yl-ethanol (pet), 3-phenoxazin-10-yl-propane-1-sulfonic acid (ppsa) and 3-phenoxazin-10-yl-propionic acid ...	2007	19071650
efficiency of new fungal cellulase systems in boosting enzymatic degradation of barley straw lignocellulose.	this study examined the cellulytic effects on steam-pretreated barley straw of cellulose-degrading enzyme systems from the five thermophilic fungi chaetomium thermophilum, thielavia terrestris, thermoascus aurantiacus, corynascus thermophilus, and myceliophthora thermophila and from the mesophile penicillum funiculosum. the catalytic glucose release was compared after treatments with each of the crude enzyme systems when added to a benchmark blend of a commercial cellulase product, celluclast, d ...	2006	16599567
phytase production by thermophilic mold sporotrichum thermophile in solid-state fermentation and its application in dephytinization of sesame oil cake.	the phytase production by sporotrichum thermophile tlr50 was recorded on all the commonly used animal feed ingredients tested to varying degrees in solid-state fermentation. enzyme production increased to 180 u/g of dry moldy residue (dmr) in sesame oil cake at 120 h and 45 degrees c at the initial substrate-to-moisture ratio of 1:2.5 and aw of 0.95. supplementation of sesame oil cake with glucose and ammonium sulfate further enhanced phytase titer (282 u/g of dmr). an overall 76% enhancement in ...	2006	16720904
thermostability of native and pegylated myceliophthora thermophila laccase in aqueous and mixed solvents.	a commercial preparation of laccase (ec 1.10.3.2), cloned from myceliophthora thermophila and expressed in aspergillus oryzae (mtl), was purified and modified by conjugation with poly(ethylene glycol) (m(r) = 5000) and is labeled peg-mtl. native enzyme was found to have a molecular mass of 80 kda, as determined by gel filtration, and 110 kda, by sds-page. the oxidative dimerization of 2,6-dimethoxyphenol (dmp) to produce the corresponding dibenzoquinone was catalyzed by mtl in a manner comparabl ...	2006	16848420
a marked enhancement in phytase production by a thermophilic mould sporotrichum thermophile using statistical designs in a cost-effective cane molasses medium.	statistical optimization of phytase production by a thermophilic mould sporotrichum thermophile in a cost-effective cane molasses medium.	2006	16882141
severe osteomyelitis caused by myceliophthora thermophila after a pitchfork injury.	traumatic injuries occurring in agricultural settings are often associated with infections caused by unusual organisms. such agents may be difficult to isolate, identify, and treat effectively.	2006	16961922
combinatorial saturation mutagenesis by in vivo overlap extension for the engineering of fungal laccases.	combinatorial saturation mutagenesis -csm- is a valuable tool for improving enzymatic properties from hot-spot residues discovered by directed enzyme evolution or performing semi-rational studies. csm coupled to a reliable high-throughput screening assay -coefficient of variance below 10%- has been used to enhance turnover rates in the fungal laccase variant t2 from myceliophthora thermophila. the influence of the highly conserved pentapeptide 509-513 on the redox potential of blue-copper contai ...	2006	17168677
nuclear amination catalyzed by fungal laccases: reaction products of p-hydroquinones and primary aromatic amines.	[reaction: see text] nuclear amination of p-hydroquinones with primary aromatic amines was catalyzed by fungal laccases (ec 1.10.3.2) from trametes spec. and myceliophthora thermophila. this is the first report of laccase-catalyzed synthesis of aminoquinones. incubation of two compounds with laccase in the presence of oxygen resulted in the formation of the corresponding monoaminated or diaminated quinones. no hydroquinonoids were formed. observed differences in the reaction courses for differen ...	2005	15760179
laccase-catalysed iodide oxidation in presence of methyl syringate.	the kinetics of potassium triiodide (ki(3)) formation during fungal laccase action was investigated in presence of methyl syringate (ms). the recombinant forms of polyporus pinsitus (rppl), myceliophthora thermophila (rmtl), coprinus cinereus (rccl), and rhizoctonia solani (rrsl) laccases were used. the triiodide formation rate reached 6.1, 5.5, 6.0, and 2.1 microm/min at saturated rppl, rccl, rrsl, and rmtl concentration, respectively, in acetate buffer solution ph 5.5 and in presence of 10 mic ...	2005	16080184
screening mutant libraries of fungal laccases in the presence of organic solvents.	reliable screening methods are being demanded by biocatalysts' engineers, especially when some features such as activity or stability are targets to improve under non-natural conditions (i.e., in the presence of organic solvents). the current work describes a protocol for the design of a fungal laccase-expressed in saccharomyces cerevisiae-highly active in organic cosolvents. a high-throughput screening assay based on abts (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)) oxidation was val ...	2005	16103414
purification and characterization of a type b feruloyl esterase (stfae-a) from the thermophilic fungus sporotrichum thermophile.	a feruloyl esterase (stfae-a) produced by sporotrichum thermophile was purified to homogeneity. the purified homogeneous preparation of native stfae-a exhibited a molecular mass of 57.0+/-1.5 kda, with a mass of 33+/-1 kda on sds-page. the pi of the enzyme was estimated by cation-exchange chromatofocusing to be at ph 3.1. the enzyme activity was optimal at ph 6.0 and 55-60 degrees c. the purified esterase was stable at the ph range 5.0-7.0. the enzyme retained 70% of activity after 7 h at 50 deg ...	2004	14615854
production, characterization and application of a thermostable polygalacturonase of a thermophilic mould sporotrichum thermophile apinis.	the production of polygalacturonase (pgase) by sporotrichum thermophile apinis in stirred submerged fermentation (smf) was high in comparison with that in static conditions. yeast extract (0.25%) and citrus pectin (2%) at ph 7.0 and 45 degrees c supported a high enzyme production in flasks agitated at 200 rpm. an overall 1.75-fold enhancement in pgase production was achieved as a result of optimization. the enzyme was optimally active at ph 7.0 and 55 degrees c, and exhibited t(1/2) of 4 h at 65 ...	2004	15182829
evaluation of certain food additives and contaminants.	this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, with a view to recommending acceptable daily intakes (adis) and to prepare specifications for the identity and purity of food additives. the first part of the report contains a general discussion of the principles governing the toxicological evaluation of food additives (including flavouring agents) and contaminants, assessments of intake, and the establishment an ...	2004	15354533
comparison of mesophilic and thermophilic feruloyl esterases: characterization of their substrate specificity for methyl phenylalkanoates.	the active sites of feruloyl esterases from mesophilic and thermophilic sources were probed using methyl esters of phenylalkanoic acids. only 13 out of 26 substrates tested were significant substrates for all the enzymes. lengthening or shortening the aliphatic side chain while maintaining the same aromatic substitutions completely abolished activity for both enzymes, which demonstrates the importance of the correct distance between the aromatic group and the ester bond. maintaining the phenylpr ...	2004	15639097
biosynthesis of fructo-oligosaccharides by sporotrichum thermophile during submerged batch cultivation in high sucrose media.	biosynthesis of fructo-oligosaccharides (fos) was observed during growth of the thermophilic fungus sporotrichum thermophile on media containing high sucrose concentrations. submerged batch cultivation with the optimum initial sucrose concentration of 250 g/l allowed the production of 12.5 g fos/l. the fos mixture obtained was composed of three sugars, which were isolated by size-exclusion chromatography. they were characterized by acid hydrolysis and hplc as 1-kestose, 6-kestose and neokestose. ...	2004	12802527
spectroscopic characterization of the leu513his variant of fungal laccase: effect of increased axial ligand interaction on the geometric and electronic structure of the type 1 cu site.	a variety of spectroscopic techniques, combined with density functional calculations, are used to describe the electronic structure of the leu513his variant of the type 1 cu site in myceliophthora thermophila laccase. this mutation changes the type 1 cu from a blue to a green site. electron paramagnetic resonance (epr), optical absorption, circular dichroism, and magnetic circular dichroism (mcd) spectroscopies reveal that, relative to the trigonal planar blue type 1 cu site in wild-type fungal ...	2003	12817956
biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of sporotrichum thermophile.	an endo-beta-1,4-xylanase (1,4-beta-d-xylan xylanoxydrolase, ec 3.2.1.8) present in culture filtrates of sporotrichum thermophile atcc 34628 was purified to homogeneity by q-sepharose and sephacryl s-200 column chromatographies. the enzyme has a molecular mass of 25,000 da, an isoelectric point of 6.7, and is optimally active at ph 5 and at 70 degrees c. thin-layer chromatography (tlc) analysis showed that endo-xylanase liberates mainly xylose (xyl) and xylobiose (xyl2) from beechwood 4-o-methyl ...	2003	12932372
amperometric biosensors based on recombinant laccases for phenols determination.	graphite (ge) or printed graphite electrode (pge) based biosensors containing recombinant fungal laccase polyporus pinsitus (rppl), and myceliophthora thermophila (rmtl) were developed. the enzymes were immobilized using bovine serum albumin and glutaraldehyde. at ph 5.5 and -0.1 v, the calibration graphs of ge based biosensors were hyperbolic if pyrocatechol was used. the concentration of substrate that results in 50% of steady-state response (ec(50)) was 0.7 mm and sensitivity (s) was 3.8 ma/m ...	2003	12485779
antimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanases.	acidic oligosaccharides were obtained from birchwood xylan by treatment with a thermoascus aurantiacus family 10 and a sporotrichum thermophile family 11 endoxylanases. the main difference between the products liberated by xylanases of family 10 and 11 concerned the length of the products containing 4-o-methyl-d-glucuronic acid. the xylanase from t. aurantiacus liberate from glucuronoxylan an aldotetrauronic acid as the shortest acidic fragment in contrast with the enzyme from s. thermophile, wh ...	2003	12568925
functional expression of a fungal laccase in saccharomyces cerevisiae by directed evolution.	laccase from myceliophthora thermophila (mtl) was expressed in functional form in saccharomyces cerevisiae. directed evolution improved expression eightfold to the highest yet reported for a laccase in yeast (18 mg/liter). together with a 22-fold increase in k(cat), the total activity was enhanced 170-fold. specific activities of mtl mutants toward 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and syringaldazine indicate that substrate specificity was not changed by the introduced mutati ...	2003	12571021
purification and characterization of a feruloyl esterase from fusarium oxysporum catalyzing esterification of phenolic acids in ternary water-organic solvent mixtures.	an extracellular feruloyl esterase (fae-ii) from the culture filtrates of fusarium oxysporum f3 was purified to homogeneity by sp-sepharose, t-butyl-hic and sephacryl s-200 column chromatography. the protein corresponded to molecular mass and pi values of 27 kda and 9.9, respectively. the enzyme was optimally active at ph 7 and 45 degrees c. the purified esterase was fully stable at ph 7.0-9.0 and temperature up to 45 degrees c after 1 h incubation. determination of k(cat)/k(m) revealed that the ...	2003	12668312
structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and ph optimum.	beta-1,4-galactanases hydrolyze the galactan side chains that are part of the complex carbohydrate structure of the pectin. they are assigned to family 53 of the glycoside hydrolases and display significant variations in their ph and temperature optimum and stability. two fungal beta-1,4-galactanases from myceliophthora thermophila and humicola insolens have been cloned and heterologously expressed, and the crystal structures of the gene products were determined. the structures are compared to t ...	2003	12761390
mode of action of family 10 and 11 endoxylanases on water-unextractable arabinoxylan.	microbial endo-beta-1,4-xylanases (exs, ec 3.2.1.8) belonging to glycanase families 10 and 11 differ in their action on water-unextractable arabinoxylan (wu-ax). wu-ax was incubated with different levels of a thermoascus aurantiacus family 10 and a sporotrichum thermophile family 11 endoxylanases. at 10 g l(-1) arabinoxylan, enzyme concentrations (ke values) needed to obtain half-maximal hydrolysis rates (v(max) values) were 4.4 nm for the xylanase from t. aurantiacus and 7.1 nm for the xylanase ...	2003	14599595
enzymic production of aldopentauronic acid and use as a bioregulator in plant airlift bioreactors.	neutral and acidic oligosaccharides were obtained from birchwood xylan by treatment with an endoxylanase, family 11 class, from sporotrichum thermophile. the main acidic xylooligosaccharide (aldopentauronic acid) was separated from the hydrolysate by anion-exchange and size-exclusion chromatography and the structure was determined by 13c nmr spectroscopy. the aldopentauronic acid yield was 25% (w/w) of the total solubilized sugars. the addition of purified aldopentauronic acid at a concentration ...	2003	16233470
formation of bound residues of 8-hydroxybentazon by oxidoreductive catalysts in soil.	this study was performed to determine which oxidoreductive catalysts were most efficient in catalyzing the binding of 8-hydroxybentazon to soil humic substances. 8-hydroxybentazon was completely transformed by an oxidoreductive enzyme, laccase of myceliophthora thermophila, at ph 3.0-7.0 within 30 min. when abiotic catalysts, manganese(iv), iron(iii), and aluminum oxides were used in the same ph range, 8-hydroxybentazon was completely transformed only by manganese(iv) oxide (delta-mno2), but a r ...	2002	12033819
toxicological studies on laccase from myceliophthora thermophila expressed in aspergillus oryzae.	the bioindustrially produced enzyme laccase can be used in different technical and food applications to facilitate processes. it can be added to different oral care products such as mouthwash, toothpaste, mints, and gums to prevent halitosis. laccase, produced by submerged fermentation of aspergillus oryzae, containing a gene originating from myceliophthora thermophila, was subject to a series of toxicological tests to document its safety in use. it was not found to be mutagenic in the salmonell ...	2002	12202045
redox-mediated decolorization of synthetic dyes by fungal laccases.	laccases from the lignin-degrading basidiomycetes trametes versicolor, polyporus pinisitus and the ascomycete myceliophthora thermophila were found to decolorize synthetic dyes to different extents. differences were attributed to the specific catalytic properties of the individual enzymes and to the structure of the dyes. due to their higher oxidative capacities, the laccases from the two basidiomycetes decolorized dyes more efficiently than that of the ascomycete. the azo dye direct red 28, the ...	2002	12226723
colorimetric assays for biodegradation of polycyclic aromatic hydrocarbons by fungal laccases.	polycyclic aromatic hydrocarbons (pahs) are highly toxic organic pollutants widely distributed in terrestrial and aquatic environments. in the present work, 2 colorimetric assays for laccase-catalyzed degradation of pahs were developed based on studies of the oxidation of 12 aromatic hydrocarbons by fungal laccases from trametes versicolor and myceliophthora thermophila. using a sodium borohydride water-soluble solution, the authors could reduce the single product of laccase-catalyzed anthracene ...	2002	14599353
dioxygen reactivity of laccase: dependence on laccase source, ph, and anion inhibition.	a study was carried out on the dioxygen reactivity of the laccases from trametes villosa, rhizoctonia solani, myceliophthora thermophila, scytalidium thermophilum, and coprinus cinereus. at ph 5.5, these laccases showed an apparently constant km (about 20-50 microm) for o2 with either 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) or methyl syringate as the reducing substrate, in contrast to the kcat, which varied up to 100-fold. o2 reactivity of the trametes and myceliophthora laccases ...	2001	11694062
a critical review of cellobiose dehydrogenases.	cellobiose dehydrogenase (cdh) is an extracellular enzyme produced by various wood-degrading fungi. it oxidizes soluble cellodextrins, mannodextrins and lactose efficiently to their corresponding lactones by a ping-pong mechanism using a wide spectrum of electron acceptors including quinones, phenoxyradicals, fe(3+), cu(2+) and triiodide ion. monosaccharides, maltose and molecular oxygen are poor substrates. cdh that adsorbs strongly and specifically to cellulose carries two prosthetic groups; n ...	2000	10725534
biophysical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): molecular size, glycosylation pattern, and engineering of proteolytic resistance.	phytases (myo-inositol hexakisphosphate phosphohydrolases) are found naturally in plants and microorganisms, particularly fungi. interest in these enzymes has been stimulated by the fact that phytase supplements increase the availability of phosphorus in pig and poultry feed and thereby reduce environmental pollution due to excess phosphate excretion in areas where there is intensive livestock production. the wild-type phytases from six different fungi, aspergillus niger, aspergillus terreus, as ...	1999	9925554
biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties.	supplementation with phytase is an effective way to increase the availability of phosphorus in seed-based animal feed. the biochemical characteristics of an ideal phytase for this application are still largely unknown. to extend the biochemical characterization of wild-type phytases, the catalytic properties of a series of fungal phytases, as well as escherichia coli phytase, were determined. the specific activities of the fungal phytases at 37 degreesc ranged from 23 to 196 u. (mg of protein)-1 ...	1999	9925555
cloning and characterization of a thermostable cellobiose dehydrogenase from sporotrichum thermophile.	cellobiose dehydrogenase (cdh) is an extracellular hemoflavoenzyme produced by several wood-degrading fungi. cdh contains one heme b and one fad per molecule and oxidizes cellobiose to cellobionolactone in the presence of cytochrome c. in this report, a thermostable cdh from the thermophilic ascomycete sporotrichum thermophile has been purified, cloned, and characterized. the temperature optimum for this cdh reaction was 60 degrees c, and the activation energy for the reaction was 26.3 kj/mol. t ...	1999	10328816
comparison of fungal laccases and redox mediators in oxidation of a nonphenolic lignin model compound.	several fungal laccases have been compared for the oxidation of a nonphenolic lignin dimer, 1-(3, 4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propan-1,3-diol (i), and a phenolic lignin model compound, phenol red, in the presence of the redox mediators 1-hydroxybenzotriazole (1-hbt) or violuric acid. the oxidation rates of dimer i by the laccases were in the following order: trametes villosa laccase (tvl) > pycnoporus cinnabarinus laccase (pcl) > botrytis cinerea laccase (bcl) > myceliophthora thermop ...	1999	10347057
site-directed mutations in fungal laccase: effect on redox potential, activity and ph profile.	a myceliophthora thermophila laccase and a rhizoctonia solani laccase were mutated on a pentapeptide segment believed to be near the type-1 cu site. the mutation l513f in myceliophthora laccase and the mutation l470f in rhizoctonia laccase took place at a position corresponding to the type-1 cu axial methionine (m517) ligand in zucchini ascorbate oxidase. the triple mutations v509l,s510e,g511a in myceliophthora laccase and l466v,e467s,a468g in rhizoctonia laccase involved a sequence segment whos ...	1998	9693103
fatal aortic myceliophthora thermophila infection in a patient affected by cystic medial necrosis.	a 22-year-old italian woman developed fungal aortitis after cardiac surgery for aortic insufficiency. she experienced two episodes of septic embolization and subsequently underwent replacement of the aortic root and initial ascending aorta by a homograft. the lumina of the ascending aorta, aortic arch and the origin of the innominate artery were completely filled with vegetation. from the involved tissue the phaeoid thermophilic hyphomycete myceliophthora thermophila (apinis) van oorschot was is ...	1998	9776822
the phytase subfamily of histidine acid phosphatases: isolation of genes for two novel phytases from the fungi aspergillus terreus and myceliophthora thermophila.	phytases catalyse the hydrolysis of phytate (myo-inositol hexakisphosphate) to myo-inositol and inorganic phosphate. in this study genes encoding novel phytases from two different filamentous fungi, aspergillus terreus strain 9a-1 and myceliophthora thermophila were isolated. the encoded phya phytase proteins show 60% (a. terreus) and 48% (m. thermophila) identity, respectively, to the phya of aspergillus niger and have 21-29% identity compared to other histidine acid phosphatases. all three phy ...	1997	9025298
characterization of the gene encoding an extracellular laccase of myceliophthora thermophila and analysis of the recombinant enzyme expressed in aspergillus oryzae.	a genomic dna segment encoding an extracellular laccase was isolated from the thermophilic fungus myceliophthora thermophila, and the nucleotide sequence of this gene was determined. the deduced amino acid sequence of m. thermophila laccase (mtl) shows homology to laccases from diverse fungal genera. a vector containing the m. thermophila laccase coding region, under transcriptional control of an aspergillus oryzae alpha-amylase gene promoter and terminator, was constructed for heterologous expr ...	1997	9251203
a study of a series of recombinant fungal laccases and bilirubin oxidase that exhibit significant differences in redox potential, substrate specificity, and stability.	a series of fungal laccases (polyporus pinsitus, rhizoctonia solani, myceliophthora thermophila, scytalidium thermophilum) and one bilirubin oxidase (myrothecium verrucaria) have been studied to determine their redox potential, specificity, and stability. polyporus and rhizoctonia laccases possess potentials near 0.7-0.8 v (vs. nhe), while other oxidases have potentials near 0.5 v. it is observed that higher redox potential correlates with higher activity. by epr, no significant change in the ge ...	1996	8597577
posttraumatic gas-containing brain abscess caused by clostridium perfringens with unique simultaneous fungal suppuration by myceliophthora thermophila: case report.	gas-containing brain abscesses are rare, and the vast majority are caused by clostridium perfringens. significant simultaneous fungal infection in a bacterial abscess is even rarer. we present such a case and review the literature.	1996	8938783
evidence for alpha and mu class glutathione s-transferases in a number of fungal species.	1. the glutathione s-transferases of the following fungal species have been affinity purified on glutathione-sepharose; yarrowia lipolytica (ncyc825), sporotrichum thermophile (atcc16479), talaromyces emersonii (atcc26914) and issatchenkia orientalis (cbs5147 and an isolate provided by prof. kumagai, kyoto university). 2. sds-page analysis of affinity purified fungal extracts, followed by western blots with polyclonal antisera to rat gsts, was performed and indicated the presence of alpha and mu ...	1993	8448996
fatal disseminated infection caused by myceliophthora thermophila, a new agent of mycosis: case history and laboratory characteristics.	we report a case of human infection caused by the hyphomycete myceliophthora thermophila. a 7-year-old male with neurofibromatosis (type 1) was diagnosed in 1987 with acute myeloblastic leukemia associated with the chromosomal abnormality monosomy 7. the patient experienced multiple serious infections over a three-year period before expiring in 1990 while in the end stage of leukemia. autopsy findings included fungal vegetations of the left atrium, ascending aorta, and pulmonary arteries and fun ...	1992	1452676
induction and regulation of alpha-amylase synthesis in a cellulolytic thermophilic fungus myceliophthora thermophila d14 (atcc 48104).	the alpha-amylase enzyme synthesis was higher when m. thermophila d-14 (atcc 48104) was grown in culture medium incorporated with starch or other carbohydrates containing maltose units. maximum enzyme production was attained with 1% starch followed by a gradual decrease with increasing concentration. marked decrease in alpha-amylase synthesis occurred with the addition of glucose to the culture medium and this decreasing activity was proportional to the concentration of glucose. the enzyme synth ...	1992	1506028
purification and characterization of extracellular β-glucosidase from myceliophthora thermophila.	the extracellular β-glucosidase has been purified from culture broth of myceliophthora thermophila atcc 48104 grown on crystalline cellulose. the enzyme was purified approximately 30-fold by (nh4)2so4 precipitation and column chromatography on deae-sephadex a-50, sephadex g-200 and deae-sephadex a-50. the molecular mass of the enzyme was estimated to be about 120 kd by both sodium dodecyl sulphate gel electrophoresis and gel filtration chromatography. it displayed optimal activity at ph 4.8 and ...	1991	24425278
fungal flora associated with combine harvester wheat and sorghum dusts from egypt.	107 species and 8 species varieties belonging to 44 genera were collected from combine harvester wheat and sorghum dusts (35 genera and 91 species + 4 varieties) and from the atmosphere of their hay sites (26 genera and 69 species + 4 varieties) on glucose- and cellulose-czapek's dox agar at 28 degrees c and 45 degrees c. the mycoflora of wheat and sorghum dusts were basically similar on the two types of media and the most common fungi were: alternaria alternata, aspergillus flavus, a. fumigatus ...	1990	2266490
purification and properties of an extracellular endoglucanase from myceliophthora thermophila d-14 (atcc 48104).	an extracellular endoglucanase (1,4-beta-glucanohydrolase, ec 3.2.1.4) produced by myceliophthora thermophila d-14 (atcc 48104) has been purified to homogeneity by ammonium sulphate precipitation and two consecutive ion-exchange chromatographic steps on deae-sephadex a-50 columns. the enzyme was purified 13.8-fold and was homogeneous by analytical page and sds-page. it has a high apparent mr, of about 100,000. the ph and temperature optima for its activity were 4.8 and 65 degrees c respectively. ...	1990	2269871
studies on the mycoflora of aswan high dam lake, egypt: monthly variations.	fifty-one species and one variety appertaining to twenty one genera of mesophilic fungi were recovered from the monthly samples of marginal water (44 species, 1 variety and 18 genera) and submerged mud (78 species, 1 variety and 30 genera) of aswan high dam lake during the period from july 1985 to december 1986. the most common species were aspergillus fumigatus, a. flavus, a. terreus, a. niger and penicillium funiculosum. the highest fungal populations were almost detected either in october, in ...	1990	2352135
production of cellulolytic enzymes by immobilized sporotrichum thermophile.	spores of sporotrichum thermophile were immobilized in agar, polyacrylamide, and sodium alginate to generate in situ mycelium for production of cellulolytic enzymes. immobilized mycelium was considerably less effective than free cells for cellulase productivity. of the three gel types, agar beads proved to be the best carrier for the immobilized spores and subsequently generated mycelium. results of repeated batch experiments suggested that the immobilized mycelia could be reused but at much red ...	1990	1366631
isolation and characterization of new thiol protease inhibitors estatins a and b.	new thiol protease inhibitors, estatins a and b, were isolated from the culture filtrate of myceliophthora thermophila m4323. the basic, water-soluble inhibitors were characterized as having an agmatine, trans-epoxysuccinic acid and l-phenylalanine or l-tyrosine moieties in the structure. the molecular formulas c18h25n5o5 and c18h25n5o6 for a and b were indicated by elemental analysis and fast atom bombardment ms. estatins were specific inhibitors against thiol proteases such as papain, ficin an ...	1989	2793591
isolation, purification and regeneration of protoplasts from sporotrichum thermophile conidiospores.	protoplasts of uniform size were prepared from mononucleated conidiospores of sporotrichum thermophile. conidia were preincubated in glucose yeast extract medium at 45 c for 4 h. the conidia were collected resuspended in buffer containing 0.6 m kcl (as stabilizer), and incubated with novozyme sp249 and cellulase cp at 37 c for 6 h. the protoplasts were separated from cell wall fragments and intact conidia by centrifugation over 50% sucrose. the purified protoplasts were regenerated in glucose ye ...	1988	3254166
induction and catabolite repression of beta-glucosidase synthesis in myceliophthora thermophila d-14 (= atcc 48104).	beta-glucosidase activity in myceliophthora thermophila d-14 (= atcc 48104) was inducible and was produced in culture filtrate during growth with various inducers, of which pnpg (p-nitrophenyl-beta-d-glucoside) was the most efficient. induction of beta-glucosidase also occurred when the organism was grown in medium supplemented with different carbon sources. carboxymethyl cellulose, cellobiose, and solka-floc were found effective for induction of enzyme biosynthesis. the addition of glucose to t ...	1988	16347725
sporotrichum thermophile growth, cellulose degradation, and cellulase activity.	the activity of components of the extracellular cellulase system of the thermophilic fungus sporotrichum thermophile showed appreciable differences between strains; beta-glucosidase (ec 3.2.1.21) was the most variable component. although its endoglucanase (ec 3.2.1.4) and exoglucanase (ec 3.2.1.91) activities were markedly lower, s. thermophile degraded cellulose faster than trichoderma reesei. the production of beta-glucosidase lagged behind that of endoglucanase and exoglucanase. the latter ac ...	1987	16347439
[thermophilic myceliophthora thermophila decomposes cellulose].	a thermophilic microscopic fungus was isolated from cattle rumen and identified as myceliophthora thermophila (apinis) van oorschot. the culture synthesized cellulolytic enzymes and xylanase when it was grown in media containing cellulase at 50 degrees c under the conditions of submerged cultivation. the morphological and physiological characteristics of the culture are described and its taxonomic position is discussed.	1983	6685815
comparative temperature-stability properties of malate dehydrogenases from some thermophilic fungi.	temperature-activity and temperature-stability relationships of malate dehydrogenases from eight thermophilic fungi were determined. temperature optima for maximum activity of the enzymes varied between 50 degrees and 60 degrees and the arrhenius plots were linear between 5 degrees and 50 degrees. the energies of activation ranged from 2.1 kcal/mol for the enzyme from sporotrichum thermophile to 9.1 kcal/mol for that from penicillium duponti. heat inactivation kinetics at 50 degrees revealed hea ...	1979	489257
stimulation of growth and glucose catabolite enzymes by succinate in some thermophilic fungi.	thermophilic humicola lanuginosa, penicillium duponti, sporotrichum thermophile and mucor pusillus required succinate in addition to glucose for optimal growth. the requirement for succinate was concentration-dependent and the concentration needed for one half of the maximal growth was 6.14mm. in the presence of succinate, glucose utilization from the medium was markedly increased and this was associated with increased levels of the enzymes of the glycolytic and krebs cycle pathways. addition of ...	1978	697502
factors influencing the production of cellulases by sporotrichum thermophile.	cellulase production and growth of a strain of sporotrichum thermophile were studied by using a mineral salts medium supplemented with yeast extract and insoluble cellulose. the effects of cultural conditions, such as ph, nitrogen source, substrate concentration, and temperature, were examined. maximum production of c1 and cx cellulases occurred at 45 c in 2 to 4 days, in the presence of 1% solka/floc as substrate, when nano3 or urea used as sources of nitrogen. under these conditions, celluloly ...	1976	7194
studies on thermophilic cellulolytic fungi.	three thermophilic cellulolytic fungi, chaetomium thermophile var. coprophile, sporotrichum thermophile, and thermoascus aurantiacus were studied to determine the conditions for a high rate of cellulose degradation. the range of temperature over which good growth occurred was determined first in a temperature gradient incubator; the optimum temperature was then established in shake flask cultures. t. aurantiacus had the highest optimum growth temperature range (46 to 51 c), whereas s. thermophil ...	1975	16350026