Publications

activation of human papillomavirus type 18 e6-e7 oncogene expression by transcription factor sp1.	the human papillomavirus 18 (hpv18) e6 and e7 proteins are considered to be primarily responsive for the transforming activity of the virus. in order to analyse the molecular mechanisms resulting in viral oncoprotein expression, it is necessary to identify the factors involved in the transcriptional regulation of the e6/e7 genes. here we define by gel retardation experiments a sequence aberrant sp1 binding site present in the promoter proximal part of the viral transcriptional control region (up ...	1992	1336181
retinoic acid-mediated repression of human papillomavirus 18 transcription and different ligand regulation of the retinoic acid receptor beta gene in non-tumorigenic and tumorigenic hela hybrid cells.	human papillomavirus type 18 (hpv18) belongs to the group of genital papillomaviruses involved in the development of cervical carcinomas. since retinoic acid (ra) is a key regulator of epithelial cell differentiation and a growth inhibitor in vitro of hpv18-positive hela cervical carcinoma cells, we have used hela and hela hybrid cells in order to analyse the effects of ra on expression of the hpv18 e6 and e7 oncogenes and of the cellular ra receptor genes rar-beta and -gamma. we show here that ...	1992	1318198
buschke-löwenstein tumor: verrucous carcinoma of the anogenitalia.	buschke-löwenstein tumor is a rare type of anogenital squamous cell carcinoma with a distinctive clinical appearance. we present the case of a thirty-four-year-old man with this tumor, which was excised surgically. human papillomavirus was detected in formalin-fixed, paraffin-embedded sections with a human papillomavirus 6/11 probe, but not with a human papillomavirus 16 or a human papillomavirus 18 probe.	1991	2070647
influence of chromosomal integration on glucocorticoid-regulated transcription of growth-stimulating papillomavirus genes e6 and e7 in cervical carcinoma cells.	in most cervical carcinoma cells the e6 and e7 genes of specific human papillomaviruses are transcribed from viral sequences integrated into host cell chromosomes. glucocorticoids activate the promoter elements of various human papillomaviruses in transient-expression assays. we have analyzed the effect of dexamethasone on the transcription rate of human papillomavirus 18 e6 and e7 genes integrated at different chromosomal sites in four cervical cancer cell lines. dexamethasone led to an increas ...	1991	1847520
identification of b-epitopes in the human papillomavirus 18 e7 open reading frame protein.	a panel of murine mab raised against a ms2 replicase/hpv 18 e7 fusion protein included 23 reactive by elisa with hpv 18 e7 determinants. a total of 19 of the 23 recognized linear epitopes in the n-terminal region of the e7 molecule, while the other four were deduced by binding inhibition assays to recognize conformational determinants in this region. all tested antibodies precipitated a 14-kda peptide doublet that corresponded with the predicted size of the e7 protein, from hela cells, but not f ...	1990	1698872
growth-regulating functions of human papillomavirus early gene products in cervical cancer cells acting dominant over enhanced epidermal growth factor receptor expression.	squamous cell carcinomas of the human anogenital tract are usually associated with infection of specific types of human papillomaviruses (hpv 16, 18, 31, 33, 35). the intracellular concentration of human papillomavirus early gene products e6 and e7 has been directly linked to the proliferative capacity of cervical cancer cells. since the expression rate of epidermal growth factor receptor correlates to growth properties in squamous carcinoma cell lines, it has been presumed that human papillomav ...	1990	1692767
histogenesis of cervical adenocarcinoma with reference to human papillomavirus-18 as a carcinogen.	human papillomavirus (hpv) dna is present in adenocarcinoma of the cervix as frequently as in squamous cell carcinoma of the cervix. recent molecular biologic studies strongly suggested that hpv acts at least as a cocarcinogen in the female genital organs. thus, a study of the presence of hpv dna in adenocarcinoma in situ, endocervical glandular dysplasia and microglandular endocervical hyperplasia, three lesions suggested as possible precursor lesions of adenocarcinoma of the cervix, would clar ...	1989	2553963
amplification of the integrated viral transforming genes of human papillomavirus 18 and its 5'-flanking cellular sequence located near the myc protooncogene in hela cells.	the human papillomavirus type 18 integrated in the hela cell genome is amplified on chromosome 8. e6, e7, and e1 open reading frames are amplified 5-fold, and the late viral dna region, the viral long control region, and cellular flanking sequences are amplified 15-fold. the common 5'-flanking cellular dna was localized by in situ hybridization of normal and hela cells only on chromosome 8 band q24. this flanking probe is included in the amplification unit of colo320 cells, but in the case of he ...	1989	2545339
characterization of primary human keratinocytes transformed by human papillomavirus type 18.	primary human epithelial cells were cotransfected with phpv-18 and psv2neo, and cell strains were generated by selecting in g418. one cell strain (fe-a), which exhibits an extended life span, is currently in its 30th passage. in comparison, control cultures can only be maintained up to the seventh passage. southern blot analysis revealed the presence of at least one intact, integrated viral genome in these cells. fe-a cells showed altered growth properties, characterized by a change in morpholog ...	1988	2452896
rearrangement of both alleles of human chromosome 8 in hela cells, one of them as a result of papillomavirus dna integration.	integration of papillomavirus in the genome of the host cell has been found associated with malignant cases of cervical carcinoma. to determine what role viral integration plays as part of the pathogenic mechanism resulting in a cancer cell, the structure of integrated papillomavirus dna (human papillomavirus dna 18) segments and its cellular flanking sequences in hela cells as well as the corresponding normal human allele have been characterized. all integrated viral dna segments have the same ...	1988	2891697
integration sites of human papillomavirus 18 dna sequences on hela cell chromosomes.	human papillomaviruses (hpv) 16 and 18 are closely linked with human genital cancer. in most cervical carcinomas, viral sequences are integrated into the host genome. hela, a cervical carcinoma cell line, has multiple copies of integrated hpv 18 dna. in this study, in situ chromosome hybridization was used to assign the integration sites of hpv 18 dna sequences on hela cell chromosomes. four sites of hybridization were identified at 8q23----q24, 9q31----q34, p11----p13 on an abnormal chromosome ...	1987	3028716
characterization of a transcriptional promoter of human papillomavirus 18 and modulation of its expression by simian virus 40 and adenovirus early antigens.	rna present in cells derived from cervical carcinoma that contained human papillomavirus 18 genomes was initiated in the 1.053-kilobase bamhi fragment that covered the complete noncoding region of this virus. when cloned upstream of the chloramphenicol acetyltransferase gene, this viral fragment directed the expression of the bacterial enzyme only in the sense orientation. initiation sites were mapped around the atg of open reading frame e6. this promoter was active in some human and simian cell ...	1987	3023691
the bpv1-e2 trans-acting protein can be either an activator or a repressor of the hpv18 regulatory region.	the human papillomavirus 18 (hpv 18) long control region contains promoter and enhancer elements whose activity is restricted to several human cell lines of epithelial origin. this enhancer possesses a considerable constitutive activity which is further stimulated in the presence of the e2 trans-activating protein of bovine papillomavirus 1 (bpv1). surprisingly the same bpv1 protein strongly repressed transcription from the genuine hpv18 enhancer-promoter dna sequences. we suggest that binding o ...	1987	2828029
chromosomal insertion of human papillomavirus 18 sequences in hela cells detected by nonisotopic in situ hybridization and reflection contrast microscopy.	genomic insertion of human papillomavirus (hpv) sequences is associated with the genesis of cervical carcinoma, and hpv-induced incipient cellular alterations may also present a requisite for the establishment of cell lines such as hela. considering the theoretical importance of specific viral integration sites, we attempted to detect in hela cells the chromosomal location of dna sequences homologous to hpv-16 and hpv-18 sequences by a nonisotopic high resolution in situ hybridization technique. ...	1987	2824333
human papillomavirus deoxyribonucleic acid in lesions of the female genital tract: evidence for type 6/11 in squamous carcinoma of the vulva.	tissue specimens from 51 patients with genital condyloma acuminata or invasive cervical or vulvar carcinomas were analyzed for the presence of human papillomavirus deoxyribonucleic acid (dna) using the dot blot technique. of ten condylomas, 80% contained dna related to human papillomavirus 6 or 11. sixty percent had evidence of dna related to human papillomavirus 16, and 30% contained dna related to human papillomavirus 18. of 24 squamous cervical carcinomas, 58% had human papillomavirus type 16 ...	1987	2819798
identification of human papillomavirus in cervical swabs by deoxyribonucleic acid in situ hybridization.	a series of 47 lesions diagnosed cytologically as cervical intraepithelial neoplasia (cin) iii in 22 cases, cin i/ii in 13 cases, and 12 cases showing abnormal smears consistent with human papillomavirus infection were analyzed. thirty-six cases with negative cytology were used as the control group. sixty-eight percent of cin iii were positive for a mixture of human papillomavirus 16 and human papillomavirus 18, 18% reacted with human papillomavirus 6 or 11, and 14% were negative. of the group w ...	1984	6095155