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photodynamic treatment with phenothiazinium photosensitizers kills both ungerminated and germinated microconidia of the pathogenic fungi fusarium oxysporum, fusarium moniliforme and fusarium solani.the search for alternatives to control microorganisms is necessary both in clinical and agricultural areas. antimicrobial photodynamic treatment (apdt) is a promising light-based approach that can be used to control both human and plant pathogenic fungi. in the present study, we evaluated the effects of photodynamic treatment with red light and four phenothiazinium photosensitizers (ps): methylene blue (mb), toluidine blue o (tbo), new methylene blue n (nmbn) and the phenothiazinium derivative s ...201627623154
genotyping of fusarium isolates from onychomycoses in colombia: detection of two new species within the fusarium solani species complex and in vitro antifungal susceptibility testing.fusariosis have been increasing in colombia in recent years, but its epidemiology is poorly known. we have morphologically and molecularly characterized 89 isolates of fusarium obtained between 2010 and 2012 in the cities of bogotá and medellín. using a multi-locus sequence analysis of rdna internal transcribed spacer, a fragment of the translation elongation factor 1-alpha (tef-1α) and of the rna-dependent polymerase subunit ii (rpb2) genes, we identified the phylogenetic species and circulatin ...201626943726
detection of human pathogenic fusarium species in hospital and communal sink biofilms by using a highly specific monoclonal antibody.the fungus fusarium is well known as a plant pathogen, but has recently emerged as an opportunistic pathogen of humans. habitats providing direct human exposure to infectious propagules are largely unknown, but there is growing evidence that plumbing systems are sources of human pathogenic strains in the fusarium solani species complex (fssc) and fusarium oxysporum species complex (fosc), the most common groups infecting humans. here, a newly developed fusarium-specific monoclonal antibody (mab ...201626914362
pathogenic ability and saline stress tolerance of two fusarium isolates from odontesthes bonariensis eggs.several fungal species represent a potential risk to embryos of odontesthes bonariensis (cuvier and valenciennes, 1835), a euryhaline freshwater fish that lives in the pampean inland waters and has potential economic relevance.201626674586
rapid identification of moulds and arthroconidial yeasts from positive blood cultures by maldi-tof mass spectrometry.moulds and arthroconidial yeasts are potential life-threatening agents of fungemia in immunocompromised patients. fast and accurate identification (id) of these pathogens hastens initiation of targeted antifungal therapy, thereby improving the patients' prognosis. we describe a new strategy that enabled the identification of moulds and arthroconidial yeasts directly from positive blood cultures by maldi-tof mass spectrometry (ms). positive blood cultures (bcs) with gram staining showing hyphae a ...201627317582
investigation of mycoviruses in endophytic and phytopathogenic strains of colletotrichum from different hosts.fungi belonging to the colletotrichum genus can be categorized as endophytic or phytopathogenic. these fungi can be infected by viruses, termed mycoviruses, which are know to promote hypovirulence in infected fungi. however, there are few studies that have described mycoviral infections of endophytes. the production of secondary metabolites by endophytes with antimicrobial potential in inhibiting numerous pathogens has gained increasing attention. the aim of the current study was to investigate ...201626985921
studying of cellular interaction of hairpin-like peptide ecamp1 from barnyard grass (echinochloa crusgalli l.) seeds with plant pathogenic fungus fusarium solani using microscopy techniques.an interaction of recombinant hairpin-like cationic peptide ecamp1 with conidia of plant pathogenic fungus fusarium solani at the cellular level was studied by a combination of microscopic methods. ecamp1 is from barnyard grass (echinochloa crusgalli l.), and obtained by heterologous expression in escherichia coli system. as a result, a direct relationship between hyphal growth inhibition and increasing active peptide concentration, time of incubation and fungal physiological condition has been ...201626855384
structural analysis of glucosylceramides (glccer) from species of the pseudallescheria/scedosporium complex.glucosylceramides (glccer) are the main neutral glycosphingolipids expressed in fungal cells. in this work, glucosylceramides (glccer) were extracted from three strains of scedosporium (pseudallescheria) boydii, one strain of pseudallescheria ellipsoidea and one strain of pseudallescheria angusta and purified by several chromatographic steps. using high-performance thin layer chromatography (hptlc), we found a similarity between glccer obtained from all of the analysed strains. a detailed struct ...201626781373
purification, biochemical characterization, and antimicrobial activity of a new lipid transfer protein from coffea canephora seeds.coffee, an agronomical crop of great economic importance, is also among the most commonly traded commodities in worldwide markets. antimicrobial peptides, which play a role in plant defense, have been identified and isolated particularly from seeds. we isolated and immunolocalized cc-ltp2, a new lipid transfer protein (ltp) from coffea canephora seeds. we report its antimicrobial activity against various phytopathogenic fungi of economic importance, and against the bacterium xanthomonas euvesica ...201627813588
antimicrobial efficacy of multipurpose disinfecting solutions in the presence of contact lenses and lens cases.the aim of this study was to use antimicrobial efficacy endpoint methodology to determine compatibility of multipurpose disinfecting solutions (mpss), lens cases, and hydrogel lenses for disinfection (aeemc) against international organization for standardization (iso)-specified microorganisms and clinical ocular isolates of stenotrophomonas maltophilia.201627598555
fungal interdigital tinea pedis in dakar (senegal).fungal interdigital tinea pedis (fitp) is the most frequent dermatomycosis in industrial countries. in african tropics, it's a rare motive of consultation and is discovered while complicated. the aims of this article were: to determine the frequency of interdigital tinea pedis among overall mycological analysis in our laboratory; to study epidemiological, clinical and mycological aspects of fitp in outpatients attending the le dantec mycology laboratory in dakar. a total of 62 males (60%) and 42 ...201627184614
bioactive metabolites produced by streptomyces cheonanensis vuk-a from coringa mangrove sediments: isolation, structure elucidation and bioactivity.the strain vuk-a was isolated from a sediment sample of the coringa mangrove ecosystem was identified as streptomyces cheonanensis based on morphological, physiological, biochemical and molecular properties. chemical investigation of the secondary metabolites of the strain streptomyces cheonanensis vuk-a has led to the segregation of two bioactive compounds, namely 2-methyl butyl propyl phthalate (1) and diethyl phthalate (2) using column chromatography. the chemical structure of the active comp ...201628330133
antifungal membranolytic activity of the tyrocidines against filamentous plant fungi.the tyrocidines and analogues are cyclic decapeptides produced by brevibacillus parabrevis with a conserved sequence of cyclo(d-phe(1)-pro(2)-x(3)-x(4)-asn(5)-gln(6)-x(7)-val(8)-x(9)-leu(10)) with trp(3,4)/phe(3,4) in the aromatic dipeptide unit, lys(9)/orn(9) as their cationic residue and tyr (tyrocidines), trp (tryptocidines) or phe (phenicidines) in position 7. previous studies indicated they have a broad antifungal spectrum with the peptides containing a tyr residue in position 7 being more ...201627328781
synthesis of pyrazolo[1,5-a]pyrimidine derivatives and their antifungal activities against phytopathogenic fungi in vitro.5,6-diarylpyrazolo[1,5-a]pyrimidines (3) and 6,7-diarylpyrazolo[1,5-a]pyrimidines (4) were chemoselectively synthesized by the condensation of isoflavone (1) and 3-aminopyrazole (2). 5,6-diarylpyrazolo[1,5-a]pyrimidines (3) were obtained via microwave irradiation, and 6,7-diarylpyrazolo[1,5-a]pyrimidines (4) were obtained via conventional heating. in addition, the pyrimidine derivatives 3 and 4 were evaluated against five phytopathogenic fungi (cytospora sp., colletotrichum gloeosporioides, botr ...201627599494
discrimination of aspergillosis, mucormycosis, fusariosis, and scedosporiosis in formalin-fixed paraffin-embedded tissue specimens by use of multiple real-time quantitative pcr assays.in a retrospective multicenter study, 102 formalin-fixed paraffin-embedded (ffpe) tissue specimens with histopathology results were tested. two 4- to 5-μm ffpe tissue sections from each specimen were digested with proteinase k, followed by automated nucleic acid extraction. multiple real-time quantitative pcr (qpcr) assays targeting the internal transcribed spacer 2 (its2) region of ribosomal dna, using fluorescently labeled primers, was performed to identify clinically important genera and spec ...201627605714
metal-based ethanolamine-derived compounds: a note on their synthesis, characterization and bioactivity.metal-based ethanolamines, (l(1))-(l(4)) coordinated with co(ii), cu(ii), ni(ii) and zn(ii) metals in 1:2 (metal:ligand) molar ratio to produce new compounds have been reported. these compounds were screened for their bactericidal/fungicidal activity against a number of bacterial (escherichia coli, shigella flexneri, pseudomonas aeruginosa, salmonella typhi, staphylococcus aureus and bacillus subtilis) and fungal strains (trichophyton longifusus, candida albicans, aspergillus flavus, microsporum ...201627557038
antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles.biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix.201627400968
effects of essential oil combinations on pathogenic yeasts and moulds.essential oils (eos) can be used as alternative or complementary antifungal agents against human pathogenic moulds and yeasts. to reduce the effective dose of antimicrobial agents, eos are combined which can lead to synergistic or additive effect. in this study the anti-yeast and anti-mould activities of selected eos were investigated, alone and in combinations, against clinical isolates of candida albicans, c. parapsilosis, aspergillus fumigatus, a. terreus, rhizopus microsporus, fusarium solan ...201627165531
etiological analysis of fungal keratitis and rapid identification of predominant fungal pathogens.fungal keratitis is a worldwide-distributed refractory and potentially blinding ocular infection caused by various fungi. it is necessary to investigate the etiological and epidemiological characteristics of this disease and establish a rapid and specific pathogenic identification method. here, we isolated and identified fungal pathogens of 275 patients with presumed fungal keratitis from jilin province, china, and conducted statistical analyses of epidemiological information. the positive rate ...201626446032
efficacy of antibody to pnag against keratitis caused by fungal pathogens.developing immunotherapies for fungal eye infections is a high priority. we analyzed fungal pathogens for expression of the surface polysaccharide, poly-n-acetyl glucosamine (pnag), and used a mouse model of ocular keratitis caused by aspergillus flavus, a. fumigatus, or fusarium solani to determine if pnag was an immunotherapy target and requirements for ancillary cellular and molecular immune effectors.201628002842
isolation and characterization of rhizosphere bacteria for the biocontrol of the damping-off disease of tomatoes in tunisia.fluorescent pseudomonas spp., isolated from tomato and pepper plants rhizosphere soil, was evaluated in vitro as a potential antagonist of fungal pathogens. pseudomonas strains were tested against the causal agents of tomatoes damping-off (sclerotinia sclerotiorum), root rot (fusarium solani), and causal agents of stem canker and leaf blight (alternaria alternata). for this purpose, dual culture antagonism assays were carried out on 25% tryptic soy agar, king b medium and potato dextrose agar to ...201624296079
identification of the non-ribosomal peptide synthetase responsible for biosynthesis of the potential anti-cancer drug sansalvamide in fusarium solani.sansalvamide is a cyclic pentadepsipeptide produced by fusarium solani and has shown promising results as potential anti-cancer drug. the biosynthetic pathway has until now remained unidentified, but here we used an agrobacterium tumefaciens-mediated transformation (atmt) approach to generate knockout mutants of two candidate non-ribosomal peptide synthetases (nrps29 and nrps30). comparative studies of secondary metabolites in the two deletion mutants and wild type confirmed the absence of sansa ...201626936154
a phenazine-1-carboxylic acid producing polyextremophilic pseudomonas chlororaphis (mcc2693) strain, isolated from mountain ecosystem, possesses biocontrol and plant growth promotion abilities.the genus pseudomonas is known to comprise a huge diversity of species with the ability to thrive in different habitats, including those considered as extreme environments. in the present study, a psychrotolerant, wide ph tolerant and halotolerant strain of pseudomonas chlororaphis gbpi_507 (mcc2693), isolated from the wheat rhizosphere growing in a mountain location in indian himalayan region (ihr), has been investigated for its antimicrobial potential with particular reference to phenazine pro ...201627394000
evaluation of antifungal efficacy of 0.1% and 0.25% riboflavin with uva: a comparative in vitro study.antifungal efficacy of photochemical cross-linking (pack-cxl) with 0.1% and 0.25% riboflavin was evaluated with a comparative in vitro study.201626644282
short communication: pharmacognostic and pharmacological evaluation of ruellia tuberosa l.ruellia tuberosa linn. of family acanthaceae was studied to investigate the microscopical, vein islet and vein termination numbers, palisade ratio, stomatal index and different chemical parameters. the antibacterial, antifungal and phytotoxic activities of the crude extract of the plant were also determined. five bacterial species were used, of which, salmonella typhi, escherichia coli, and pseudomonas aeruginosa were the most susceptible bacterial species to crude extract with mics 10, 4.0 and ...201628375130
novel bio-essential metal based complexes linked by heterocyclic ligand: synthesis, structural elucidation, biological investigation and docking analysis.new series of bio-essential metal based complexes linked by schiff base ligand (l) and 2,2'-bipyridine (bpy) have been synthesized and characterized by diverse spectral techniques such as elemental analysis, magnetic susceptibility, molar conductivity measurements, ft-ir, uv-vis., (1)h nmr, (13)c nmr, epr and mass. the spectral data suggest that the metal complexes espouse octahedral geometry around the metal ions. interactions of the complexes with ct dna have been explored by electronic absorp ...201626690017
cytotoxic and antibiotic cyclic pentapeptide from an endophytic aspergillus tamarii of ficus carica.a new cyclic pentapeptide, disulfide cyclo-(leu-val-ile-cys-cys) (1), named malformin e, together with 13 known cyclic dipeptides, was isolated from the culture broth of endophytic fungus fr02 from the roots of ficus carica. the strain fr02 was identified as aspergillus tamarii on the basis of morphological characteristics and molecular analyses of internal transcribed spacer (its). their structures were determined by the combination of 1d and 2d nmr spectroscopy, hrms (esi), uv, and marfey's an ...201627147299
termitarium-inhabiting bacillus endophyticus tsh42 and bacillus cereus tsh77 colonizing curcuma longa l.: isolation, characterization, and evaluation of their biocontrol and plant-growth-promoting activities.bacillus strains were isolated from termitarium soil and screened for their antifungal activity through the production of diffusible and volatile metabolites. further, the bacterial strains that showed antifungal activity were evaluated for their biocontrol potential on the basis of their plant-growth-promoting attributes. termitarium-inhabiting bacillus strains tsh42 and tsh77 significantly reduced the growth of pathogenic fungus fusarium solani, controlled the symptoms of rhizome rot in turmer ...201627604298
modulation of phytoremediation and plant growth by the treatment with pgpr, ag nanoparticle and untreated municipal wastewater.the present attempt was made to determine the effects of untreated municipal wastewater (mw) on growth and physiology of maize and to evaluate the role of ag nanoparticle and plant-growth-promoting rhizobacteria (pgpr) when interacting with mw used for irrigation. it was used for the isolation of pgpr. the isolates were identified and characterized based on the colony morphology, c/n source utilization pattern using miniaturized identification system (qts 24), catalase (cat) and oxidase tests, a ...201627348506
synthesis and evaluation of ester derivatives of 10-hydroxycanthin-6-one as potential antimicrobial agents.as part of our continuing research on canthin-6-one antimicrobial agents, a new series of ester derivatives of 10-hydroxycanthin-6-one were synthesized using a simple and effective synthetic route. the structure of each compound was characterized by nmr, esi-ms, ft-ir, uv, and elemental analysis. the antimicrobial activity of these compounds against three phytopathogenic fungi (alternaria solani, fusarium graminearum, and fusarium solani) and four bacteria (bacillus cereus, bacillus subtilis, ra ...201627007362
isolation and characterization of bacterial endophytes of curcuma longa l.fourteen endophytic bacterial isolates were isolated from the rhizome of curcuma longa l. were characterized on the basis of morphology, biochemical characteristics and 16s rrna gene sequence analysis. the isolates were identified to six strains namely bacillus cereus (ecl1), bacillus thuringiensis (ecl2), bacillus sp. (ecl3), bacillus pumilis (ecl4), pseudomonas putida (ecl5), and clavibacter michiganensis (ecl6). all the strains produced iaa and solubilized phosphate and only two strains produ ...201628330130
effect of seed pelleting with biocontrol agents on growth and colonisation of roots of mungbean by root-infecting fungi.mungbean (vigna radiata (l.) wilczek) is a leguminous pulse crop that is a major source of proteins, vitamins and minerals. root-infecting fungi produce severe plant diseases like root rot, charcoal rot, damping-off and stem rot. the soil-borne pathogens can be controlled by chemicals, but these chemicals have several negative effects. use of microbial antagonist such as fungi and bacteria is a safe, effective and eco-friendly method for the control of many soil-borne pathogens. biological contr ...201626619828
use of a sec signal peptide library from bacillus subtilis for the optimization of cutinase secretion in corynebacterium glutamicum.technical bulk enzymes represent a huge market, and the extracellular production of such enzymes is favorable due to lowered cost for product recovery. protein secretion can be achieved via general secretion (sec) pathway. specific sequences, signal peptides (sps), are necessary to direct the target protein into the translocation machinery. for example, >150 sec-specific sps have been identified for bacillus subtilis alone. as the best sp for a target protein of choice cannot be predicted a prio ...201627927208
antifungal efficiency of a lipopeptide biosurfactant derived from bacillus subtilis spb1 versus the phytopathogenic fungus, fusarium solani.bacillus subtilis spb1 lipopeptides were evaluated as a natural antifungal agent against fusarium solani infestation. in vitro antifungal assay showed a minimal inhibitory concentration of about 3 mg/ml with a fungicidal mode of action. in fact, treatment of f. solani by spb1 lipopeptides generated excessive lyses of the mycelium and caused polynucleation and destruction of the related spores together with a total inhibition of spore production. furthermore, an inhibition of germination potency ...201526178831
antimicrobial and allelopathic metabolites produced by penicillium brasilianum.six known compounds, isoroquefortine c (1), griseofulvin (2), ergosterol peroxide (3), 3β-hydroxy-(22e,24r)-ergosta-5,8,22-trien-7-one (4), cerevisterol (5) and (22e,24r)-6β-methoxyergosta-7,22-diene-3β,5α-diol (6), were produced by the fungus penicillium brasilianum, and their structures were elucidated by spectroscopic methods. this is the first report on isoroquefortine c as naturally occurring compound. their bioactivities against five phytopathogenic fungi (gibeberalla saubinetti, fusarium ...201525103127
filamentous fungal endophthalmitis: results of combination therapy with intravitreal amphotericin b and voriconazole.to report outcomes of exogenous fungal endophthalmitis treated with combination of intravitreal antifungal agents.201525926714
biocontrol of tomato plant diseases caused by fusarium solani using a new isolated aspergillus tubingensis ctm 507 glucose oxidase.the present study focuses on the potential of glucose oxidase (god) as a promising biocontrol agent for fungal plant pathogens. in fact, a new god producing fungus was isolated and identified as an aspergillus tubingensis. god (125 au) has been found to inhibit fusarium solani growth and spore production. indeed, god caused the reduction of spores, the formation of chlamydospores, the induction of mycelial cords and the vacuolization of mycelium. in vivo assays, god acted as a curative treatment ...201526299190
1,1-diphenyl,2-picrylhydrazyl free radical scavenging, bactericidal, fungicidal and leishmanicidal properties of teucrium stocksianum.in the present study, we evaluated the pharmacological and toxicological effects of teucrium stocksianum. the crude extract of t. stocksianum (ts.cr) and its subsequent organic fractions: n-hexane (ts.hex), chloroform (ts.chcl3) and ethyl acetate (ts.etac) exhibited 1,1-diphenyl,2-picrylhydrazyl free radical scavenging activity with different potencies. ts.etac was found to be most potent. ts.cr, ts.hex, ts.chcl3 and ts.etac showed significant bactericidal activity against escherichia coli, stap ...201523625908
a single-step purification of cauliflower lysozyme and its dual role against bacterial and fungal plant pathogens.a novel lysozyme from cauliflower was purified in a single step, for the first time, using sephadex g100 column chromatography. the purified lysozyme exhibited a homogenized single band in sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), and its molecular mass was calculated to be 22.0 kda. the purified lysozyme showed activity between 30 to 60 °c with 40 °c as the optimum temperature for its maximal activity. although the purified lysozyme was functional at ph ranges betwee ...201526208688
ruta montana l. leaf essential oil and extracts: characterization of bioactive compounds and suppression of crown gall disease.the aims of this study were to assess the antimicrobial efficacy of the leaf essential oil and the leaf extracts of r. montana against botrytis cinerea, fusarium oxysporum, verticillium dahliae, aspergillus oryzae and fusarium solani. the oil (1.000 µg/disk) and the extracts (1.500 µg/disk) revealed a remarkable antifungal effect against the tested plant pathogenic fungi with a radial growth inhibition percentage of 40.0-80.0 % and 5.0-58.0 %, respectively along with their respective mic values ...201526417353
antimicrobial, antitumor and brine shrimp lethality assay of ranunculus arvensis l. extracts.to investigate the antitumor activity, brine shrimp lethality assay, antibacterial and antifungal activity of methanol extract (me), water extract (we), acetone extract (ae), chloroform extract (ce), methanol-water extract (mwe), methanol-acetone extract (mae), methanol-chloroform extract (mce) of ranunculus arvensis (l.). antitumor activity was evaluated with agrobacterium tumefaciens (at10) induced potato disc assay. cytotoxicity was evaluated with brine shrimp lethality assay. antibacterial a ...201526004705
fungal isolation from disinfectant solutions of contact lens storage cases among asymptomatic users.to investigate the incidence of fungal contamination of store disinfectant solutions contained in contact lens storage cases belonging to asymptomatic contact lens users.201525503912
fungal infections after boston type 1 keratoprosthesis implantation: literature review and in vitro antifungal activity of hypochlorous acid.to review the current literature describing cases of fungal keratitis and endophthalmitis after boston keratoprosthesis (kpro) implantation and to characterize the antifungal activity of 0.01% hypochlorous acid against medically relevant fungi.201526488624
sertraline enhances the activity of antimicrobial agents against pathogens of clinical relevance.serotonin reuptake inhibitors were recently reported to possess antimicrobial potentials, potentiate activity of several antibiotics, reverse multidrug resistant phenotypes of bacteria and make them susceptible to previously resistant drugs. we investigated antimicrobial potentials of sertraline (sr) against atcc strains, clinical isolates of staphylococcus aureus, escherichia coli and pseudomonas aeruginosa alone and in-combination with seven antibiotics. antifungal activity was investigated ag ...201526029671
antibacterial, antifungal, and insecticidal potentials of oxalis corniculata and its isolated compounds.oxalis corniculata is a common medicinal plant widely used against numerous infectious diseases. the agrochemical potential of methanolic extract, n-hexane, chloroform, ethyl acetate, and n-butanol fractions were assessed to measure the antibacterial, antifungal, and insecticidal activities of the plant. the crude, chloroform, and n-butanol soluble fractions showed excellent activities against escherichia coli, shigella dysenteriae, salmonella typhi, and bacillus subtilis but have no activity ag ...201525873973
design, synthesis and in vitro bactericidal/fungicidal screening of some vanadyl(iv)complexes with mono- and di-substituted ons donor triazoles.a new series of anti-bacterial and anti-fungal mono- and di-substituted triazoles (l(1))-(l(6)) have been synthesized and characterized on the basis of their physical, spectral and analytical data. the ligands (l(1))-(l(6)) on reaction with vanadyl(iv) sulphate led to the formation of vanadyl(iv) metal complexes (1)-(4). the structure of the complexes has been established on the basis of their physical, spectral and elemental analyses data. the synthesized ligands and their vanadyl(iv) complexes ...201525716124
synthesis of heterocycle-attached methylidenebenzenesulfonohydrazones as antifungal agents.a series of heterocycle-attached methylidenebenzenesulfonohydrazone derivatives were synthesized and evaluated for their antifungal activities against seven phytopathogenic fungi such as fusarium graminearum, alternaria solani, valsa mali, phytophthora capsici, fusarium solani, botrytis cinerea, and glomerella cingulata. compounds 7b, 8d, 9a, 9b and 9d exhibited a good and broad-spectrum of antifungal activities against at least five phytopathogenic fungi at the concentration of 100 μg/ml. it de ...201526471091
broad-spectrum antimicrobial/antifouling soft material coatings using poly(ethylenimine) as a tailorable scaffold.microbial colonization and biofilm formation is the leading cause of contact lens-related keratitis. treatment of the condition remains a challenge because of the need for prolonged therapeutic course and high doses of antimicrobial agents especially for biofilm eradication. the development of strategies to prepare nonfouling contact lens surfaces is a more practical way to ensure users' safety and relieve the excessive public healthcare burden. in this study, we report a series of polymers that ...201526039032
new constitutive latex osmotin-like proteins lacking antifungal activity.proteins that share similar primary sequences to the protein originally described in salt-stressed tobacco cells have been named osmotins. so far, only two osmotin-like proteins were purified and characterized of latex fluids. osmotin from carica papaya latex is an inducible protein lacking antifungal activity, whereas the calotropis procera latex osmotin is a constitutive antifungal protein. to get additional insights into this subject, we investigated osmotins in latex fluids of five species. ...201526231325
antagonistic activities of novel peptides from bacillus amyloliquefaciens pt14 against fusarium solani and fusarium oxysporum.bacillus species have recently drawn attention due to their potential use in the biological control of fungal diseases. this paper reports on the antifungal activity of novel peptides isolated from bacillus amyloliquefaciens pt14. reverse-phase high-performance liquid chromatography revealed that b. amyloliquefaciens pt14 produces five peptides (pt14-1, -2, -3, -4a, and -4b) that exhibit antifungal activity but are inactive against bacterial strains. in particular, pt14-3 and pt14-4a showed broa ...201526496638
studies on drug metabolism by fungi colonizing decomposing human cadavers. part ii: biotransformation of five model drugs by fungi isolated from post-mortem material.the present study investigated the in vitro metabolic capacity of 28 fungal strains isolated from post-mortem material towards five model drugs: amitriptyline, metoprolol, mirtazapine, promethazine, and zolpidem. each fungal strain was incubated at 25 °c for up to 120 h with each of the five models drugs. cunninghamella elegans was used as positive control. aliquots of the incubation mixture were centrifuged and 50 μl of the supernatants were diluted and directly analyzed by liquid chromatograph ...201524898183
[identification of pathogens causing root rot disease on ligusticum chuanxiong in sichuan].to identify the pathogens causing root rot disease on ligusticum chuanxiong from the main producing areas in sichuan, and to provide gist of management of this disease in the future.201526495640
diversity of endophytic fungi in glycine max.endophytic fungi are microorganisms that live within plant tissues without causing disease during part of their life cycle. with the isolation and identification of these fungi, new species are being discovered, and ecological relationships with their hosts have also been studied. in glycine max, limited studies have investigated the isolation and distribution of endophytic fungi throughout leaves and roots. the distribution of these fungi in various plant organs differs in diversity and abundan ...201526111593
structural and functional studies of a fusarium oxysporum cutinase with polyethylene terephthalate modification potential.cutinases are serine hydrolases that degrade cutin, a polyester of fatty acids that is the main component of plant cuticle. these biocatalysts have recently attracted increased biotechnological interest due to their potential to modify and degrade polyethylene terephthalate (pet), as well as other synthetic polymers.201526291558
biotechnological prospecting of foliar endophytic fungi of guaco (mikania glomerata spreng.) with antibacterial and antagonistic activity against phytopathogens.mikania glomerata (spreng.), popularly known as "guaco", is a plant from the asteraceae family that has many therapeutic properties. the use of medicinal plants has been examined in studies on endophytic diversity and bioprospecting; endophytes inhabit the interior of plants without harming them. microorganism-host complex interactions are related to the production of compounds that may confer resistance to pathogens or to production of bioactive compounds or growth regulators. in this study, we ...201526214408
candida and fusarium species known as opportunistic human pathogens from customer-accessible parts of residential washing machines.energy constraints have altered consumer practice regarding the use of household washing machines. washing machines were developed that use lower washing temperatures, smaller amounts of water and biodegradable detergents. these conditions may favour the enrichment of opportunistic human pathogenic fungi. we focused on the isolation of fungi from two user-accessible parts of washing machines that often contain microbial biofilms: drawers for detergents and rubber door seals. out of 70 residentia ...201525749362
identification of different fusarium spp. in allium spp. in germany.in 2013 allium cepa bulbs from different fields in northern and southern germany, seeds and sets from onion breeders were analysed for infestation with fusarium species. the same investigation was done in 2014 with different edible allium spp. from local markets. different fusarium spp. were isolated and identified by morphological characterisation. 24 different fusarium spp. were identified. the diversity of fusarium spp. and the intensity of infestation was higher on edible bulbs compared to t ...201527141742
[molecular identification and in vitro susceptibility of fusarium from fungal keratitis in central china].to detect the genotypes and in vitro antifungal susceptibility of fusarium isolated from patients with fungal keratitis in central china.201526693650
role of antagonistic microorganisms and organic amendment in stimulating the defense system of okra against root rotting fungi.without application of chemical pesticides control of soilborne diseases is a great challenge. stimulation of natural plant's defense is considered as one of the most promising alternative strategy for crop protection. organic amendment of soil besides direct suppressing the pathogen, has been reported to have an influence on phytochemicals in plants. in the present study, pseudomonas aeruginosa, a plant growth promoting rhizobacterium and paecilomyces lilacinus, an egg parasite of root knot and ...201526373176
phylogenetic diversity of human pathogenic fusarium and emergence of uncommon virulent species.fusarium species cause a broad spectrum of infections. however, little is known about the etiological agents to the species level. we identified fusarium species isolated from clinical specimens including those of high risk patients to better understand the species involved in the pathogenesis.201526348828
emergence of fusarioses in a university hospital in turkey during a 20-year period.fusarium species have started appearing increasingly as the main cause of infections, particularly in immunocompromised patients. in this study, we aimed to present the first epidemiological data from turkey, analyze fusariosis cases that have been monitored in a university hospital during the past 20 years, identify the responsible fusarium species, and determine antifungal susceptibilities. a total of 47 cases of fusariosis was included in the study. fusarium isolates were identified by multil ...201525994844
disseminated fusariosis and hematologic malignancies, a still devastating association. report of three new cases.fungi of the genus fusarium are primarily plant pathogens and saprobes that produce disseminated infections in immunologically deficient humans. after aspergillosis, disseminated fusariosis is the second most common cause of invasive infection by filamentous fungi in patients with hematologic malignancies or those undergoing transplants of hematopoietic progenitors.201525936697
isolation, purification, and characterization of a stable defensin-like antifungal peptide from trigonella foenum-graecum (fenugreek) seeds.a novel defensin-like antifungal peptide (tf-afp) with molecular mass of 10.3 kda was isolated from seeds of trigonella foenum-graecum (fenugreek) by ammonium sulfate precipitation, cation-exchange, gel-filtration, hydrophobic chromatography, and rp-hplc. mass spectroscopic analysis revealed the intact mass of the purified antifungal peptide as 10321.5 da and high similarity to plant defensins and other antifungal proteins in database search. 2d-page showed pi value to be 8.8 and absence of isof ...201525761687
fusarium diversity in soil using a specific molecular approach and a cultural approach.fusarium species are ubiquitous in soil. they cause plant and human diseases and can produce mycotoxins. surveys of fusarium species diversity in environmental samples usually rely on laborious culture-based methods. in the present study, we have developed a molecular method to analyze fusarium diversity directly from soil dna. we designed primers targeting the translation elongation factor 1-alpha (ef-1α) gene and demonstrated their specificity toward fusarium using a large collection of fungi. ...201525655778
fungal peritonitis due to fusarium solani species complex sequential isolates identified with dna sequencing in a kidney transplant recipient in brazil.fungal peritonitis is a rare serious complication most commonly observed in immunocompromised patients under peritoneal dialysis. nevertheless, this clinical condition is more difficult to treat than bacterial peritonitis. bacterial peritonitis followed by the use of antibiotics is the main risk factor for developing fungal peritonitis. candida spp. are more frequently isolated, and the isolation of filamentous fungi is only occasional. here we describe a case of fusarium solani species complex ...201526296944
fusarium brain abscess: case report and literature review.severely immunocompromised patients such as those with haematological malignancies and haematopoietic stem cell transplant recipients are at an increased risk of acquiring invasive mould infections. fusarium, a ubiquitous fungus, can cause potentially fatal infections in such hosts. it usually manifests as skin lesions, fevers and sino-pulmonary infections. brain abscesses have been reported, but are relatively uncommon. we report a case of a 50-year-old patient with acute lymphocytic leukaemia ...201525476184
non-host disease resistance response in pea (pisum sativum) pods: biochemical function of drr206 and phytoalexin pathway localization.continually exposed to potential pathogens, vascular plants have evolved intricate defense mechanisms to recognize encroaching threats and defend themselves. they do so by inducing a set of defense responses that can help defeat and/or limit effects of invading pathogens, of which the non-host disease resistance response is the most common. in this regard, pea (pisum sativum) pod tissue, when exposed to fusarium solani f. sp. phaseoli spores, undergoes an inducible transcriptional activation of ...201525457488
antagonistic potential of native trichoderma viride strain against potent tea fungal pathogens in north east india.indigenous strains of trichoderma species isolated from rhizosphere soils of tea gardens of assam, north eastern state of india were assessed for in vitro antagonism against two important tea fungal pathogens namely pestalotia theae and fusarium solani. a potent antagonist against both tea pathogenic fungi, designated as sdrlin1, was selected and identified as trichoderma viride. the strain also showed substantial antifungal activity against five standard phytopathogenic fungi. culture filtrate ...201526361476
inhibition of hmg-coa reductase by mfs, a purified extract from the fermentation of marine fungus fusarium solani fg319, and optimization of mfs production using response surface methodology.the present study was designed to isolate and characterize a purified extract from fusarium solani fg319, termed mfs (metabolite of fusarium solani fg319) that showed anti-atherosclerosis activity by inhibiting 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase. response surface methodology (rsm) was employed to achieve an improved yield from the fermentation medium. the inhibiting effect of the isolate, mfs, on hmg-coa reductase was greater than that of the positive control, lovastatin. ...201525986283
[fungicidal properties of the nanosystems of silkworm (bombyx mor) chitosan with copper ions].the fungicidal activity of some chitosan samples and of its nanostructured systems with copper was studied. the samples of bombyx mori chitosan and its nanostructured systems with copper were found to inhibit growth and development of phytopathogenic fungi fusarium solani 169 and verticillium dahlie 57. the growth inhibition zone was of considerable size (22 to 60 mm).201525941714
[tinea pedis due to fusarium solani in dakar].a patient presented with intertrigo at the second, third and fourth interdigitals spaces lasting for four years in which fusarium solani was highlighted. the search for contributing factors revealed a concept of foot washing with water at least five times a day for ablutions, associated with wearing closed shoes all day and the absence of immunosuppression and diabetes. the diagnosis of fusarium was made on the basis of direct examination and culture. combined treatment with griseofulvin oral an ...201525835156
coculnol, a new penicillic acid produced by a coculture of fusarium solani fki-6853 and talaromyces sp. fka-65. 201525712393
isolation and characterization of two mitoviruses and a putative alphapartitivirus from fusarium spp.the filamentous fungus fusarium spp. includes several important plant pathogens. we attempted to reveal presence of double-stranded (ds) rnas in the genus. thirty-seven fusarium spp. at the maff collection were analyzed. in the strains of fusarium coeruleum, fusarium globosum and fusarium solani f. sp. pisi, single dsrna bands were detected. the strains of f. coeruleum and f. solani f. sp. pisi cause potato dry rot and mulberry twig blight, respectively. sequence analyses revealed that dsrnas in ...201525687123
morphological and comparative genomic analyses of pathogenic and non-pathogenic fusarium solani isolated from dalbergia sissoo.sissoo or shisham (dalbergia sissoo roxb.) is one of the finest wood of south asia. fusarium solani is a causal organism of sissoo wilt, decline, or dieback. it is also a potential causal organism associated with other valuable tree species. thirty-eight fusarium isolates including 24 f. solani and 14 fusarium sp., were obtained in 2005 from different geographical locations in india. all 38 (18 pathogenic and 20 non-pathogenic) isolates were characterized for genomic analysis, growth behaviour, ...201525605046
enhanced camptothecin production by ethanol addition in the suspension culture of the endophyte, fusarium solani.ethanolic extract of a non-camptothecin producing plant, catharanthus roseus when added in the suspension culture of the endophyte fusarium solani known to produce camptothecin, resulted in enhanced production of camptothecin by 10.6-fold in comparison to that in control (2.8 μg/l). interestingly, addition of pure ethanol (up to 5% v/v) in the suspension culture of f. solani resulted in maximum enhancement in camptothecin production (up to 15.5-fold) from that obtained in control. in the presenc ...201525603728
in silico analysis and prioritization of drug targets in fusarium solani.mycotic keratitis has emerged as a major ophthalmic problem and a leading cause of blindness, since its recognition in 1879. filamentous fungi are major causative of mycotic keratitis. in india, the main etiological organism responsible for mycotic keratitis is aspergillus species followed by fusarium species. in south india, fusarium based keratitis scales up to 43%. nearly one-third of mycotic keratitis treatment results in failure, as fungal infections are highly resistant to antibiotic thera ...201525555413
a substrate fed-batch biphasic catalysis process for the production of natural crosslinking agent genipin with fusarium solani accc 36223.the natural crosslinking agent genipin has been applied widely in biomedicines and foods nowadays. because of the special hemiacetal ring structure in its molecule, it can only be prepared by hydrolysis of geniposide according to biocatalysis. in this research, strategies including aqueous-organic biphasic catalysis and substrate fed-batch mode were adopted to improve the biocatalysis process of genipin. a 10 l ethyl acetate-aqueous biphasic system with geniposide fed-batch led to a satisfying g ...201525537719
the galactolipase activity of fusarium solani (phospho)lipase.the purified (phospho)lipase of fusarium solani (fsl), was known to be active on both triglycerides and phospholipids. this study aimed at assessing the potential of this enzyme in hydrolyzing galactolipids. fsl was found to hydrolyze at high rates of synthetic medium chains monogalactosyldiacylglycerol (4658±146u/mg on dic8-mgdg) and digalactosyldiacylglycerol (3785±83u/mg on dic8-dgdg) and natural long chain monogalactosyldiacylglycerol extracted from leek leaves (991±85u/mg). it is the microb ...201525529980
multidrug resistant fusarium keratitis.we report a case of keratitis in a female contact lens wearer, who developed a deep corneal abscess. the culture of a corneal biopsy scraping was positive for multiresistant fusarium solani. the patient has a complicated clinical course and failed to respond to local and systemic antifungal treatment, requiring eye enucleation.201525443198
fatal disseminated infection with fusarium petroliphilum.members of the fusarium solani species complex (fssc) are causing the majority of the fusariosis in humans. disseminated fusariosis has a high mortality and is predominantly observed in patients with leukemia. here, we present the case of a fatal infection by a fusarium strain with a degenerated phenotype, in a patient with acute lymphatic leukemia. multiple nasal and skin biopsies as well as blood cultures yielded fungal growth, while in direct and histopathological examination of biopsy materi ...201525234793
fusarium keratitis at a tertiary eye care centre in india.the aim of the study was to report the clinical and microbiological profiles of fusarium keratitis. in this single-centre, retrospective, non-comparative case series, 47 laboratory-confirmed cases of keratitis caused by fusarium species treated at the l v prasad eye institute, bhubaneswar, india, between november 2006 and october 2009, were reviewed. the analysis included predisposing factors, clinical characteristics, microbiological findings, treatment and outcome. forty-seven samples of 47 pa ...201524929671
novel taxa in the fusarium fujikuroi species complex from pinus spp.the pitch canker pathogen fusarium circinatum has caused devastation to pinus spp. in natural forests and non-natives in commercially managed plantations. this has drawn attention to the potential importance of fusarium species as pathogens of forest trees. in this study, we explored the diversity of fusarium species associated with diseased pinus patula, p. tecunumanii, p. kesiya and p. maximinoi in colombian plantations and nurseries. plants displaying symptoms associated with a f. circinatum- ...201526955193
inhibitory effects of antimicrobial agents against fusarium species.we investigated the inhibitory effects of antibacterial, biocidal, and antifungal agents against fusarium spp. seven fusarium spp: four f. falciforme (fusarium solani species complex), one fusarium spp, one fusarium spp. (fusarium incarnatum-equiseti species complex), and one f. napiforme (gibberella fujikuroi species complex), isolated from eyes with fungal keratitis were used in this study. their susceptibility to antibacterial agents: flomoxef, imipenem, gatifloxacin, levofloxacin, moxifloxac ...201525841054
spectrum of fusarium infections in tropical dermatology evidenced by multilocus sequencing typing diagnostics.fusarium species are emerging causative agents of superficial, cutaneous and systemic human infections. in a study of the prevalence and genetic diversity of 464 fungal isolates from a dermatological ward in thailand, 44 strains (9.5%) proved to belong to the genus fusarium. species identification was based on sequencing a portion of translation elongation factor 1-alpha (tef1-α), rdna internal transcribed spacer and rna-dependent polymerase subunit ii (rpb2). our results revealed that 37 isolat ...201525530264
morphological and phylogenetic analysis of fusarium solani species complex in malaysia.members of fusarium solani species complex (fssc) have been known as plant, animal, and human pathogens. nevertheless, the taxonomic status of such an important group of fungi is still very confusing and many new species as well as lineages have been elucidated recently. unfortunately, most of the new taxa came from temperate and subtropical regions. therefore, the objectives of the present study were to identify strains of fssc recovered from different sources in malaysia. in the present study, ...201525238930
a comparative analysis of distribution and conservation of microsatellites in the transcripts of sequenced fusarium species and development of genic-ssr markers for polymorphism analysis.we used an in silico approach to survey and compare microsatellites in transcript sequences of four sequenced members of genus fusarium. g + c content of transcripts was found to be positively correlated with the frequency of ssrs. our analysis revealed that, in all the four transcript sequences studied, the occurrence, relative abundance and density of microsatellites varied and was not influenced by transcript sizes. no correlation between relative abundance and transcript sizes was observed. ...201526269381
evaluation of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry bruker biotyper for identification of penicillium marneffei, paecilomyces species, fusarium solani, rhizopus species, and pseudallescheria boydii.we evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms), the maldi bruker biotyper system (microflex lt; bruker daltonik gmbh, bremen, germany), on the identification of 50 isolates of clinically encountered molds, including penicillium marneffei (n = 28), paecilomyces species (n = 12), fusarium solani (n = 6), rhizopus species (n = 3), and pseudallescheria boydii (n = 1). the isolates were identified to species levels by sequen ...201526217315
selection and molecular identification of fungal isolates that produce xylanolytic enzymes.xylan is a heteropolysaccharide and its complete hydrolysis involves a complex set of xylanolytic enzymes. fungal xylanases have been widely used in the holocellulose industry to obtain by-products or for its elimination. the aim of this study was to select and identify filamentous fungi from different ecosystems that produce extracellular xylanases showing biotechnological potential. one hundred three fungal isolates were obtained from orchard, horticultural, and forest ecosystems. the ability ...201526214493
endophytic bacteria from piper tuberculatum jacq.: isolation, molecular characterization, and in vitro screening for the control of fusarium solani f. sp piperis, the causal agent of root rot disease in black pepper (piper nigrum l.).endophytic bacteria have been found to colonize internal tissues in many different plants, where they can have several beneficial effects, including defense against pathogens. in this study, we aimed to identify endophytic bacteria associated with roots of the tropical piperaceae piper tuberculatum, which is known for its resistance to infection by fusarium solani f. sp piperis, the causal agent of black pepper (piper nigrum) root rot disease in the amazon region. based on 16s rrna gene sequence ...201526214435
bioprocess development for docosahexaenoic acid from novel fungal isolate of fusarium solani.fungal cultures were isolated from soil samples collected from the western ghats regions of kerala. primary screening of isolated strains were done by sudan black staining method and 15 lipid producing cultures were isolated. the fatty acid profiling of the positive strains were analyzed for docosahexaenoic acid (dha) production. selected oleaginous cultures were grown in submerged culture condition to study the biomass yield and poly unsaturated fatty acid, dha production. the optimization of p ...201526155676
anatomy of a nonhost disease resistance response of pea to fusarium solani: pr gene elicitation via dnase, chitosan and chromatin alterations.of the multiplicity of plant pathogens in nature, only a few are virulent on a given plant species. conversely, plants develop a rapid "nonhost" resistance response to the majority of the pathogens. the anatomy of the nonhost resistance of pea endocarp tissue against a pathogen of bean, fusarium solani f.sp. phaseoli (fsph) and the susceptibility of pea to f. solani f sp. pisi (fspi) has been described cytologically, biochemically and molecular-biologically. cytological changes have been followe ...201526124762
fatal fusarium solani species complex infections in elasmobranchs: the first case report for black spotted stingray (taeniura melanopsila) and a literature review.fusarium species are environmental saprophytic fungi. among the many fusarium species, members of the fusarium solani species complex (fssc) are the most prevalent and virulent in causing human and animal infections. in this study, we describe the first case of fatal fssc infection in a black spotted stingray and three concomitant infections in scalloped hammerhead sharks. in the stingray, cutaneous lesions were characterised by ulcers and haemorrhage of the ventral pectoral fin, or 'ray', espec ...201526095191
screen for soil fungi highly resistant to dichloroaniline uncovers mostly fusarium species.arylamines are frequent pollutants in soils. fungi have proven to be efficient in detoxifying these chemicals by acetylating them using arylamine n-acetyl transferase enzymes. here, we selected from natural soils fungi highly resistant to 3,4-dichloroaniline (dca). fusarium species were the most frequently isolated species, especially fusarium solani. the sequenced strain of f. solani contains five nat genes, as did all the dca-resistant isolates. rt-pcr analysis showed that the five genes were ...201526068093
effects of fusarium solani and f. oxysporum infection on the metabolism of ginsenosides in american ginseng roots.american ginseng (panax quinquefolius l.) is a highly valuable herb widely used for medicinal treatments. its pharmacologically important compounds are the ginsenosides, which are secondary metabolites in american ginseng root. the concentrations of ginsenoside in roots can be changed by fungal infection, but it is unclear what specific root tissues are impacted and whether the change is systemic. in this study, american ginseng roots were inoculated with two fungal pathogens (fusarium solani or ...201526060917
three new cutinases from the yeast arxula adeninivorans that are suitable for biotechnological applications.the genes acut1, acut2, and acut3, encoding cutinases, were selected from the genomic dna of arxula adeninivorans ls3. the alignment of the amino acid sequences of these cutinases with those of other cutinases or cutinase-like enzymes from different fungi showed that they all had a catalytic s-d-h triad with a conserved g-y-s-q-g domain. all three genes were overexpressed in a. adeninivorans using the strong constitutive tef1 promoter. recombinant 6× his (6h)-tagged cutinase 1 protein (p) from a ...201526048925
production of 4-ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of ipomoea batatas lam.: a comparative study.a comparative study was done on the production of 4-ipomeanol from root tubers of ipomoea batatas and its rhizogenic callus. best callusing response was obtained on ms medium supplemented with 11 μm naa (α-naphthaleneacetic acid) and 1 μm kin (kinetin). effect of various elicitors (fusarium solani, chitin and chitosan) on the production of 4-ipomeanol was studied. methanol extract of the samples were purified by column chromatography and detected using tlc. identification of 4-ipomeanol was conf ...201526040027
crystal structure of an antifungal osmotin-like protein from calotropis procera and its effects on fusarium solani spores, as revealed by atomic force microscopy: insights into the mechanism of action.cposm is an antifungal osmotin/thaumatin-like protein purified from the latex of calotropis procera. the protein is relatively thermostable and retains its antifungal activity over a wide ph range; therefore, it may be useful in the development of new antifungal drugs or transgenic crops with enhanced resistance to phytopathogenic fungi. to gain further insight into the mechanism of action of cposm, its three-dimensional structure was determined, and the effects of the protein on fusarium solani ...201526456062
molecular identification of fusarium species isolated from transgenic insect-resistant cotton plants in mexicali valley, baja california.cotton production in the mexicali valley is adversely affected by wilt and root rot disease associated with fusarium species. in the present study, we sought to isolate and identify the fusarium species in the rhizosphere of transgenic insect-resistant cotton plants grown in the mexicali valley. our analyses isolated four native fungi from the rhizosphere of cotton plants, namely, t-ica01, t-ica03, t-ica04, and t-ica08. these fungal isolates were categorized as belonging to fusarium solani using ...201526436498
expression of vitamin d receptor and cathelicidin in human corneal epithelium cells during fusarium solani infection.to observe the expression of vitamin d receptor (vdr) in human specimen and immortalized human corneal epithelium cells (hcec) when challenged with fusarium solani. moreover, we decided to discover the pathway of vdr expression. also, we would like to detect the expression of cathelicidin antimicrobial peptide (camp) in the downstream pathway of vdr.201526558193
status of the effectiveness of contact lens solutions against keratitis-causing pathogens.the aim of this study was to assess the antimicrobial effects of marketed contact lens disinfecting solutions.201525280981
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