Publications

an accessory open reading frame (orf-x) of jaagsiekte sheep retrovirus is conserved between different virus isolates.	jaagsiekte sheep retrovirus (jsrv) is the etiological agent of a contagious lung tumour of sheep known as sheep pulmonary adenomatosis (syn: ovine pulmonary carcinoma, jaagsiekte). jsrv exhibits a simple genetic organization, characteristic of the type d and type b retroviruses, with the canonical retroviral sequences gag, pro, pol and env encoding the structural proteins of the virion. an additional open reading frame (orf-x), of approximately 500 bp overlapping pol, is present in the only two ...	2000	10653922
the c-myc locus is a common integration site in type b retrovirus-induced t-cell lymphomas.	type b leukemogenic virus (tblv) induces rapidly appearing t-cell leukemias. tblv insertions near the c-myc gene were detectable in 2 of 30 tumors tested, whereas 80% of the tumors showed c-myc overexpression. tblv insertions on chromosome 15 (including a newly identified locus, pad7) may cause c-myc overexpression by cis-acting effects at a distance.	2000	10666282
role of matrix protein in the type d retrovirus replication cycle: importance of the arginine residue at position 55.	we previously reported that a mutant of mason-pfizer monkey virus (m-pmv), which has an amino acid substitution in the matrix (ma) protein at position 55, ma-r55w, showed altered viral morphogenesis, reduced glycoprotein incorporation, and loss of infectivity. in this report, we show that two additional amino acid substitutions at this site in ma, r55f and r55y, also result in similar altered morphogenesis, env incorporation, and infectivity, demonstrating that these changes are not specific for ...	2000	10704360
a novel shuttle protein binds to rna helicase a and activates the retroviral constitutive transport element.	the constitutive transport element (cte) of type d retroviruses mediates the nuclear export of unspliced viral transcripts. we previously showed that rna helicase a functionally interacts with cte and contains a bidirectional nuclear transport domain at the carboxyl terminus. here we report the identification of a novel human protein, helicase a-binding protein 95 (hap95), which specifically binds to the carboxyl terminus of rna helicase a. hap95 is partially homologous to akap95, a member of th ...	2000	10748171
retrovirus vectors bearing jaagsiekte sheep retrovirus env transduce human cells by using a new receptor localized to chromosome 3p21.3.	jaagsiekte sheep retrovirus (jsrv) is a type d retrovirus associated with a contagious lung tumor of sheep, ovine pulmonary carcinoma. other than sheep, jsrv is known to infect goats, but there is no evidence of human infection. until now it has not been possible to study the host range for jsrv because of the inability to grow this virus in culture. here we show that the jsrv envelope protein (env) can be used to pseudotype moloney murine leukemia virus (momlv)-based retrovirus vectors and that ...	2000	10775607
the long terminal repeat of jaagsiekte sheep retrovirus is preferentially active in differentiated epithelial cells of the lungs.	jaagsiekte sheep retrovirus (jsrv) is the etiologic agent of a contagious bronchioloalveolar carcinoma of sheep known as sheep pulmonary adenomatosis (spa; ovine pulmonary carcinoma). jsrv is unique among retroviruses because it transforms the alveolar type ii cells and the nonciliated bronchiolar cells (clara cells) of the lungs; these cells are where jsrv is specifically expressed in both naturally and experimentally spa-affected sheep. in this study, we investigated the cell specificity of js ...	2000	10846056
specific interaction between rna helicase a and tap, two cellular proteins that bind to the constitutive transport element of type d retrovirus.	constitutive transport element (cte) facilitates retroviral rna export by interacting with the cellular rna export machinery. two cellular proteins, rna helicase a (rha) and tip-associated protein (tap) were identified as binding to cte and were proposed to function as cte co-factors (1,2). here, we report that these two cte-binding proteins interact with each other in vitro and in vivo. the in vitro binding of rha to tap is direct and independent of either cte or the nuclear transport domain of ...	2000	10924507
sam68, rna helicase a and tap cooperate in the post-transcriptional regulation of human immunodeficiency virus and type d retroviral mrna.	unlike cellular mrna, retroviral mrna bypasses the tight coupling of the splicing and nuclear export steps to allow the export of intron-containing viral rna transcripts to the cytoplasm. two distinct nuclear export pathways for retroviral mrna have been described: a crm-1 dependent pathway mediated by the hiv-1 rev protein and the rev response element (rre), and a crm-1 independent pathway mediated by the constitutive transport element (cte) of type d retroviruses. two cte-binding proteins, rna ...	2000	10951562
sheep pulmonary adenomatosis: characterization of two pathological forms associated with jaagsiekte retrovirus.	pathological and immunohistochemical studies were performed on the lungs of 10 sheep with lesions of "classical" sheep pulmonary adenomatosis (spa) and six sheep with "atypical" lung tumours. lung tumour samples and other tissues from the same 16 animals were tested for the presence of jaagsiekte retrovirus (jsrv) by a polymerase chain reaction (pcr) that amplified a portion of the u3 long terminal repeat. the differences in the gross appearance of the classical and atypical forms paralleled the ...	2000	10627391
porcine endogenous retroviral mrnas in pancreas and a panel of tissues from specific pathogen-free pigs.	pigs are potential providers of donor tissues for xenotransplantation (e.g. of pancreatic islets) in type 1 diabetes. in this context, our group has studied the use of islets from specific pathogen-free (spf) pigs as a means of reducing the risks of "conventional zoonosis". although this approach does not prevent the transmission of pig endogenous retrovirus (perv) to humans, we attempted to determine the presence of c-type perv mrnas for gag, pol, and env subtypes as a first descriptive step in ...	1999	10633879
complete sequence of enzootic nasal tumor virus, a retrovirus associated with transmissible intranasal tumors of sheep.	the sequence of the complete genome of ovine enzootic nasal tumor virus, an exogenous retrovirus associated exclusively with contagious intranasal tumors of sheep, was determined. the genome is 7,434 nucleotides long and exhibits a genetic organization characteristic of type b and d oncoviruses. enzootic nasal tumor virus is closely related to the jaagsiekte sheep retrovirus and to sheep endogenous retroviruses.	1999	10196294
jaagsiekte retrovirus is widely distributed both in t and b lymphocytes and in mononuclear phagocytes of sheep with naturally and experimentally acquired pulmonary adenomatosis.	jaagsiekte sheep retrovirus (jsrv) is a type d retrovirus specifically associated with a contagious lung tumor of sheep, sheep pulmonary adenomatosis (spa). jsrv replicates actively in the transformed epithelial cells of the lung, and jsrv dna and rna have been detected in lymphoid tissues of naturally affected animals. to determine the lymphoid target cells of jsrv, cd4(+) t cells, cd8(+) t cells, b lymphocytes, and adherent cell (macrophage/monocyte) populations were isolated from the mediasti ...	1999	10196296
novel endogenous type d retroviral particles expressed at high levels in a scid mouse thymic lymphoma.	a xenograft model of the human disease langerhans cell histiocytosis (lch) was investigated with severe combined immunodeficiency (scid) mice. transplantation of human lch biopsy material into scid mice resulted in the generation of mouse tumors resembling lymphomas. a thymoma cell line (thye1m6) was generated from one of these mice and found to display significant levels of mg2+-dependent reverse transcriptase activity. electron microscopy revealed particles with type d retroviral morphology bu ...	1999	10233925
improved mg2+-based reverse transcriptase assay for detection of primate retroviruses.	the reverse transcriptase (rt) assay is a simple, relatively inexpensive, widely used assay that can detect all retroviruses (known and novel retroviruses as well as infectious and defective retroviruses) on the basis of the divalent cation requirement of their rt enzyme, i.e., mg2+ or mn2+. descriptions of various rt assays have been published; however, they cannot be directly applied to the analysis of biological products or clinical samples without further standardization to determine the low ...	1999	10325311
jaagsiekte sheep retrovirus is necessary and sufficient to induce a contagious lung cancer in sheep.	sheep pulmonary adenomatosis (spa) is a contagious and experimentally transmissible lung cancer of sheep resembling human bronchiolo-alveolar carcinoma. a type d retrovirus, known as jaagsiekte sheep retrovirus (jsrv), has been associated with the etiology of spa, but its exact role in the induction of the tumor has not been clear due to the lack of (i) a tissue culture system for the propagation of jsrv and (ii) an infectious jsrv molecular clone. to investigate the role of jsrv in the etiology ...	1999	10400795
identification of novel import and export signals of human tap, the protein that binds to the constitutive transport element of the type d retrovirus mrnas.	the nuclear export of the unspliced type d retrovirus mrna depends on the cis-acting constitutive transport rna element (cte) that has been shown to interact with the human tap (htap) protein promoting the export of the cte-containing mrnas. we report here that htap is a 619-amino-acid protein extending the previously identified protein by another 60 residues at the n terminus and that htap shares high homology with the predicted rat and mouse tap proteins. we found that htap is a nuclear protei ...	1999	10454577
in vitro infection of ovine cell lines by jaagsiekte sheep retrovirus.	sheep pulmonary adenomatosis (spa), also known as jaagsiekte or ovine pulmonary carcinoma, is a contagious lung cancer of sheep, originating from type ii pneumocytes and clara cells. previous studies have implicated a type d retrovirus (jaagsiekte sheep retrovirus [jsrv]) as the causative agent of spa. we recently isolated a proviral clone of jsrv from an animal with a spontaneous case of spa (jsrv(21)) and showed that it harbors an infectious and oncogenic virus. this demonstrated that jsrv is ...	1999	10559321
seroprevalence of specific viral infections in confiscated orangutans (pongo pygmaeus).	a serological survey of confiscated orangutans was conducted to determine the prevalence of specific viral infections cross reacting with human viruses. antibodies specific for human hepatitis a (hav) and b (hbv) viruses, herpes simplex viruses (hsv), and human t-lymphotropic virus (htlv types i and ii), as well as for the simian type d retroviruses (srv types 1 to 3) and simian immunodeficiency virus (siv) were tested in samples from 143 orangutans. results revealed a high prevalence of potenti ...	1998	9606041
lack of a specific immune response against a recombinant capsid protein of jaagsiekte sheep retrovirus in sheep and goats naturally affected by enzootic nasal tumour or sheep pulmonary adenomatosis.	enzootic nasal tumour (ent) and sheep pulmonary adenomatosis (spa) are two contagious adenocarcinomas of the respiratory tract of sheep and goats. both diseases are associated with related, but distinct, type-d-retroviruses (entv and jsrv respectively). no evidence of circulating antibodies has been described in animals affected by either ent or spa using antigens from natural sources. we evaluated the usefulness of a recombinant jsrv capsid protein (jsrv-ca) as antigen to study the antibody res ...	1998	9613437
isolated leptomeningeal castleman's disease with viral particles in the follicular dendritic cells.	to our knowledge, five cases of castleman's disease involving only the central nervous system have been reported previously. we report a sixth case, which occurred in a 47-year-old woman with a 3-month history of headaches and a large superior frontal lobe mass on neuroimaging. excisional biopsy revealed confluent lymphoid nodular areas with multiple well-developed germinal centers surrounded by concentrically layered proliferations of small b lymphocytes typical of castleman's disease. ultrastr ...	1998	9822134
siv hunt leads to baboon virus discovery.		1997	9157548
a cellular cofactor for the constitutive transport element of type d retrovirus.	a human nuclear protein that specifically interacts with the constitutive transport element (cte) of simian retrovirus was identified as adenosine 5'-triphosphate-dependent rna helicase a. this protein could bind to functional cte but not to inactive cte mutants. the interaction of helicase a with cte was distinct from previously described helicase activity of this protein. helicase a shuttled from the nucleus to the cytoplasm in the presence of a transcription inhibitor or in cells transiently ...	1997	9162007
sheep pulmonary adenomatosis: a unique model of retrovirus-associated lung cancer.	sheep pulmonary adenomatosis (spa) is a contagious bronchiolo-alveolar carcinoma of sheep associated with an exogenous type d/b retrovirus known as jaagsiekte sheep retrovirus (jsrv). spa represents a unique model for lung cancer, and studies on its aetiopathogenesis can provide further insight into the mechanisms of epithelial neoplasms.	1997	9447659
distribution of endogenous type b and type d sheep retrovirus sequences in ungulates and other mammals.	the jaagsiekte sheep retrovirus (jsrv), which appears to be a type b/d retrovirus chimera, has been incriminated as the cause of ovine pulmonary carcinoma. recent studies suggest that the sequences related to this virus are found in the genomes of normal sheep and goats. to learn whether there are breeds of sheep that lack the endogenous viral sequences and to study their distribution among other groups of mammals, we surveyed several domestic sheep and goat breeds, other ungulates, and various ...	1996	8622932
the exogenous form of jaagsiekte retrovirus is specifically associated with a contagious lung cancer of sheep.	sheep pulmonary adenomatosis ([spa] ovine pulmonary carcinoma) is a transmissible lung cancer of sheep that has been associated etiologically with a type d- and b-related retrovirus (jaagsiekte retrovirus (jsrv]). to date it has been impossible to cultivate jsrv in vitro and therefore to demonstrate the etiology of spa by a classical approach. in addition, the presence of 15 to 20 copies of endogenous jsrv-related sequences (enjsrv) has hampered studies at the molecular level. the aim of this st ...	1996	8627682
endogenous d-type (herv-k) related sequences are packaged into retroviral particles in the placenta and possess open reading frames for reverse transcriptase.	all primates studied to date produce retroviral-like particles in their placentae. we have purified these particles from two primate species, one old world (human) and one new world (marmoset), and have identified the retroviral sequences which are packaged into these particles. three families of sequences have been detected in these particles in human, all of which have the highest homology to b- and d-type retroviruses and to the human endogenous retrovirus herv-k10. previous studies have repo ...	1996	8806530
pcr-based detection and partial characterization of a retrovirus associated with contagious intranasal tumors of sheep and goats.	a type d-related retrovirus has been demonstrated in enzootic nasal tumors (ents) of sheep and goats. this retrovirus, ent virus (entv), has antigenic cross-reactivity with the jaagsiekte sheep retrovirus (jsrv), which is associated with a contagious lung tumor of sheep (sheep pulmonary adenomatosis). here, we present the first report of nucleic acid sequence from entv which confirms, at the nucleic acid level, that this retrovirus is related to jsrv yet apparently distinct from it. reverse tran ...	1996	8892877
jaagsiekte retrovirus establishes a disseminated infection of the lymphoid tissues of sheep affected by pulmonary adenomatosis.	jaagsiekte retrovirus (jsrv) is an exogenous type d-related retrovirus specifically associated with a contagious lung cancer of sheep (sheep pulmonary adenomatosis; spa). recently, epithelial tumour cells in the lungs of spa-affected sheep were identified as major sites of jsrv replication by immunological techniques and rt-pcr amplification of part of jsrv gag. jsrv was not detected outside the lungs and their draining lymph nodes. however, low levels of jsrv expression in non-respiratory tissu ...	1996	9000089
characterization of enzootic nasal tumor virus capsid antigen.	the rt-pcr was carried out on tumor tissue from sheep with enzootic nasal tumor (ent), using primers designed from conserved amino acid regions of related type d retroviruses. a 591 bp pcr fragment, corresponding to 90% of the capsid antigen was cloned, sequenced and expressed in e. coli. alignment with ovine pulmonary carcinoma (opc) virus showed 93% nucleotide and 96% amino acid homology. no amplification occurred when dna from ovine fetal cell line was used as template. the recombinant protei ...	1996	9008337
retrovirus-like particles released from the human breast cancer cell line t47-d display type b- and c-related endogenous retroviral sequences.	the human mammary carcinoma cell line t47-d releases retrovirus-like particles of type b morphology in a steroid-dependent manner (i. keydar, t. ohno, r. nayak, r. sweet, f. simoni, f. weiss, s. karby, r. mesa-tejada, and s. spiegelman, proc. natl. acad. sci. usa 81:4188-4192, 1984). furthermore, reverse transcriptase (rt) activity is found to be associated with particle preparations. using a set of degenerate primers derived from a conserved region of retroviral pol genes, we repeatedly amplifi ...	1995	7545247
epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for jaagsiekte retrovirus.	sheep pulmonary adenomatosis (spa) is a naturally occurring contagious lung tumour of sheep which has been associated aetiologically with a type d- and b-related retrovirus (jaagsiekte retrovirus; jsrv). to improve understanding of the aetio-pathogenesis of spa, the distribution and the sites of jsrv replication in sheep with naturally or experimentally induced spa or in unaffected controls were identified. new immunological reagents were produced and a blocking enzyme-linked immunosorbent assay ...	1995	7595380
transmissible retrovirus in epstein-barr virus-producer b95-8 cells.	epstein-barr virus (ebv) released from the b95-8 marmoset cell line has served as a prototype for biologic and biochemical studies of ebv. here we identify and characterize a retrovirus carried by many cultures of b95-8 cells. the experiments were stimulated by the isolation of a cdna clone from b95-8 cells in which sequences from the ebv large internal repeat were linked to gag sequences similar to those of squirrel monkey retrovirus, human isolate, smrv-h. however, among 413 amino acids predic ...	1995	7778272
phosphorothioate oligonucleotides inhibit the replication of lentiviruses and type d retroviruses, but not that of type c retroviruses.	phosphorothioate analogs of oligodeoxynucleotides at a concentration of 2 microm protected himalayan tahr cells from infection by caprine arthritis encephalitis virus (caev) and equine dermis cells from infection by equine infectious anemia virus (eiav). the characteristics of this inhibition against these lentiviruses are similar to those previously described for the inhibition of hiv-1 in ath8 cells [17]. thus, the 28-mer homo-oligomer of cytidine [s-(dc)28] was at least as effective as three ...	1994	7826217
identification of human type d retrovirus as a contaminant in a neuroblastoma cell line.	in this report we describe a type d virus isolated from a human neuroblastoma cell line (paju). the viral rna was isolated, partially molecularly cloned and sequenced. our clones were shown to be identical to a human d-type retrovirus previously isolated from a human lymphoblastoid cell line. however, we obtained no evidence for the virus in earlier passage of the paju cell line and therefore we must consider this isolate a laboratory contamination. contamination.	1993	8385922
purification and biochemical characterization of squirrel monkey retrovirus-h produced in a human lymphoblastoid cell line.	we found a type d retrovirus in a human lymphoblastoid cell line of b-cell lineage. molecular cloning and nucleotide sequencing of the provirus genome revealed that this virus was closely related to squirrel monkey retrovirus (smrv), and we designated this virus as smrv-h. to investigate the relationship between these retroviruses, smrv-h was purified from the virus-producing cells, and its biochemical properties were characterized. the cell-adhesive virus particles were successfully separated f ...	1992	1282848
reticuloendotheliosis type c and primate type d oncoretroviruses are members of the same receptor interference group.	the reticuloendotheliosis viruses (revs), originally isolated from avian species, constitute a group of retroviruses which are more closely related to mammalian retroviruses than to other avian retroviruses. the envelope glycoproteins of members of the rev group display a striking amino acid sequence identity with a group of primate oncoretroviruses which belong to a single receptor interference group and include all of the type d and some type c primate oncoretroviruses. members of the rev grou ...	1992	1316460
identification of a generalised packaging sequence for d-type retroviruses and generation of a d-type retroviral vector.	in order to construct vectors based upon d-type, rather than c-type, retroviruses, we have identified a 624-bp fragment of mason-pfizer monkey virus (mpmv) which constitutes a packaging sequence for at least two d-type retroviruses. when this fragment was included in an extensively deleted d-type vector genome, the d-type viruses mpmv and srv-5, but not the c-type viruses mlv-a or mlv-e, rescued the vector rna from hela cells. the recombinant virus stocks have the host range of the rescuing d-ty ...	1992	1322603
a single amino acid substitution within the matrix protein of a type d retrovirus converts its morphogenesis to that of a type c retrovirus.	two different morphogenic processes of retroviral capsid assembly have been observed: the capsid is either assembled at the plasma membrane during the budding process (type c), or preassembled within the cytoplasm (types b and d). we describe here a gag mutant of mason-pfizer monkey virus, a type d retrovirus, in which a tryptophan substituted for an arginine in the matrix protein results in efficient assembly of capsids at the plasma membrane through a morphogenic process similar to that of typ ...	1990	2170021