Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| contribution of streptococcus anginosus to infections caused by groups c and g streptococci, southern india. | vellore, a region in southern india, has a high incidence of severe human infections with beta-hemolytic group c and g streptococci (gcgs). to determine the causative species in these infections, we conducted 16s rrna gene sequencing: streptococcus dysgalactiae subsp. equisimilis (81%) and s. anginosus (19%) were the causative organisms in the 2-year study period (2006-2007). we used pcr to detect the virulence-related emm gene; results showed that it was restricted to s. dysgalactieae subsp. eq ... | 2010 | 20350380 |
| streptococcus dysgalactiae subsp. equisimilis bacteremia, finland, 1995-2004. | we conducted a retrospective population-based study of 140 episodes of streptococcus dysgalactiae subsp. equisimilis bacteremia occurring in finland during 1995-2004. rare emm types were associated with more severe disease and increased mortality rates. skin and soft tissue infections were more frequent clinical signs among cases caused by common emm types. | 2010 | 20409380 |
| distribution of emm types and genetic characterization of the mgc locus in group g streptococcus dysgalactiae subsp. equisimilis from a hospital in northern taiwan. | a total of 274 streptococcus dysgalactiae subsp. equisimilis isolates was analyzed by emm typing and by determining the organization of their mgrc loci. three of the most frequent emm types were stg485.0 (45/274, 16.4%), stg6.1 (43/274, 15.7%), and stc839.0 (32/274, 11.7%), in decreasing order. the cpdb-positive mgrc locus appears to be predominant in some emm types. | 2010 | 20573871 |
| molecular detection of streptococcus pyogenes and streptococcus dysgalactiae subsp. equisimilis. | we developed molecular diagnostic assays for the detection of streptococcus pyogenes (gas) and streptococcus dysgalactiae subsp. equisimilis (sdse), two streptococcal pathogens known to cause both pharyngitis and more invasive forms of disease in humans. two real-time pcr assays coupled with an internal control were designed to be performed in parallel. one assay utilizes a gene target specific to gas, and the other utilizes a gene target common to the two species. both assays showed 2-3 orders ... | 2009 | 19156548 |
| a novel integrative conjugative element mediates genetic transfer from group g streptococcus to other {beta}-hemolytic streptococci. | lateral gene transfer is a significant contributor to the ongoing evolution of many bacterial pathogens, including beta-hemolytic streptococci. here we provide the first characterization of a novel integrative conjugative element (ice), icesde3396, from streptococcus dysgalactiae subsp. equisimilis (group g streptococcus [ggs]), a bacterium commonly found in the throat and skin of humans. icesde3396 is 64 kb in size and encodes 66 putative open reading frames. icesde3396 shares 38 open reading f ... | 2009 | 19168609 |
| genetic relationships deduced from emm and multilocus sequence typing of invasive streptococcus dysgalactiae subsp. equisimilis and s. canis recovered from isolates collected in the united states. | beta-hemolytic group c and g streptococci cause a considerable invasive disease burden and sometimes cause disease outbreaks. little is known about the critical epidemiologic parameter of genetic relatedness between isolates. we determined the emm types of 334 streptococcus dysgalactiae subsp. equisimilis isolates, and attempted emm typing of 5 streptococcus canis isolates from a recent population-based surveillance for invasive isolates. thirty-four emm types were observed, including one from s ... | 2009 | 19386831 |
| incidence of lameness and abrasions in piglets in identical farrowing pens with four different types of floor. | lameness in piglets is a major animal welfare issue. floor abrasiveness is a common cause of superficial injury in piglets in farrowing pens. the abrasion achieved may act as a gate for infections, which in turn may induce development of infectious arthritis. in this study, the influence of improvements of the floor quality and of increased ratios of straw in identical farrowing pens was measured. | 2009 | 19463187 |
| acute bacterial, nonnecrotizing cellulitis in finland: microbiological findings. | bacterial, nonnecrotizing cellulitis is a localized and often recurrent infection of the skin. the aim of this study was to identify the beta-hemolytic streptococci that cause acute nonnecrotizing cellulitis infection in finland. | 2008 | 18260753 |
| genetic features of clinical isolates of streptococcus dysgalactiae subsp. equisimilis possessing lancefield's group a antigen. | thirteen streptococcus dysgalactiae subsp. equisimilis isolates possessing lancefield's group a antigen recovered from people in japan during 2000 to 2004 were genotyped. the results indicate that a conserved clone has persisted and spread within japan, and two different emm types were observed within members of this clone. | 2008 | 18305132 |
| fatal group c streptococcal infection due to transfusion of a bacterially contaminated pooled platelet unit despite routine bacterial culture screening. | an elderly man with chronic myelomonocytic leukemia developed respiratory distress and died less than 48 hours after transfusion of a pool of eight whole blood-derived platelets (plts). blood cultures from the recipient and cultures of remnants from the pooled plt bag grew group c streptococci (gcs). an investigation was conducted to identify both the infection's source and the reasons for the false-negative screening result. | 2008 | 18564393 |
| spondylodiscitis and paraspinal abscess caused by beta-haemolytic group g streptococci spreading from infected leg ulcers. | we report a case of spondylodiscitis due to streptococcus dysgalactiae subsp. equisimilis spreading from infected leg ulcers. the route of infection could be unequivocally demonstrated by culturing identical isolates from leg wounds, blood culture and intra-surgery specimens from the spine. the present case illustrates the pathogenic potential of group g streptococci also for non-diabetic adults. | 2008 | 18719188 |
| [case of gas gangrene in both legs due to streptococcus dysgalactiae subsp. equisimilis, resulting in amputation of right leg]. | 2008 | 18788420 | |
| rare codon priority and its position specificity at the 5' of the gene modulates heterologous protein expression in escherichia coli. | rare codons and their effects in heterologous protein expression in escherichia coli were addressed by many investigators. here, we propose that not all rare codons of a foreign gene have negative effect but selective codon among them and its specific position in the downstream of the start codon modulates the expression. in our study, streptokinase (47 kda), encoded by skc gene of streptococcus equisimilis was expressed in e.coli. the analysis of relative codon frequency of skc gene in e.coli r ... | 2008 | 18801340 |
| [streptococcus equisimilis associated septic arthritis/prosthetic joint infection]. | group c streptococci are flora members of skin, nasopharynx, gastrointestinal and genitourinary systems. they are rare causes of human pharyngitis, arthritis, pneumonia, meningitis and bacteremia. in this report, a 71-years old male patient with streptococcus equisimilis arthritis/prosthetic joint infection has been presented. the patient was admitted to the emergency service with the complaints of erythema, swelling and tenderness on right knee which had total knee prosthesis. examination of sy ... | 2008 | 18822898 |
| biosorption of cr(vi) by three different bacterial species supported on granular activated carbon: a comparative study. | the ability of three different bacterial species supported on granular activated carbon (gac) to remove hexavalent chromium from low concentration liquid solutions was investigated, in batch and column studies. the microorganisms tested were cr(vi) reducing types: streptococcus equisimilis (cect 926), bacillus coagulans (cect 12) and escherichia coli (cect 515). the results showed metal uptake values of 5.82, 5.35 and 4.12 mg/g(bios.), respectively, for s. equisimilis, b. coagulans and e. coli, ... | 2008 | 17933461 |
| metabolic engineering of escherichia coli for biosynthesis of hyaluronic acid. | engineering of hyaluronic acid (ha) biosynthetic pathway in recombinant escherichia coli as production host is reported in this work. a hyaluronic acid synthase (has) gene, sphasa, from sreptococcus pyogenes with the start codon gtg to atg mutant, was expressed in recombinant e. coli with or without the genes ugd, galf and glmu, which are analogs of hasb, hasc and hasd from streptococcus, respectively, encoding udp-glucose 6-dehygrogenase, glucose-1-p uridyltransferase, and n-acetyl glucosamine ... | 2008 | 17959405 |
| beta-lactam failure in treatment of two group g streptococcus dysgalactiae subsp. equisimilis pharyngitis patients. | we present two cases of exudative pharyngitis due to streptococcus dysgalactiae subsp. equisimilis, lancefield group g. while the participation of this organism as an agent of pharyngitis is well documented, we focus on failure of beta-lactam therapy, a phenomenon that is well described for pharyngitis due to streptococcus pyogenes. therefore, these case reports add to our knowledge of pharyngitis caused by non-s. pyogenes streptococci. | 2008 | 18057124 |
| identification of tet(s) gene area in tetracycline-resistant streptococcus dysgalactiae subsp. equisimilis clinical isolates. | 2008 | 18156606 | |
| lysogenic transfer of group a streptococcus superantigen gene among streptococci. | a group a streptococcus (gas) isolate, serotype m12, recovered from a patient with streptococcal toxic shock syndrome was analyzed for superantigen-carrying prophages, revealing phi149, which encodes superantigen ssa. sequence analysis of the att-l proximal region of phi149 showed that the phage had a mosaic nature. remarkably, we successfully obtained lysogenic conversion of gas clinical isolates of various m serotypes (m1, m3, m5, m12, m19, m28, and m94), as well as of group c streptococcus eq ... | 2008 | 18179387 |
| epidemiology of streptococcus dysgalactiae subsp. equisimilis in tropical communities, northern australia. | streptococcus dysgalactiae subsp. equisimilis (groups c and g streptococci [gcs/ggs]) is an increasingly recognized human pathogen, although it may follow indirect pathways. prospective surveillance of selected households in 3 remote aboriginal communities in australia provided 337 gcs/ggs isolates that were emm sequence-typed. lancefield group c isolates (gcs) were localized to specific households and group g isolates (ggs) were more evenly distributed. gcs/ggs was more frequently recovered fro ... | 2007 | 18217553 |
| macrolide resistance trends in beta-hemolytic streptococci in a tertiary korean hospital. | erythromycin-resistant beta-hemolytic streptococci (bhs) has recently emerged and quickly spread between and within countries throughout the world. in this study, we evaluate the antimicrobial susceptibility patterns and erythromycin resistance mechanisms of bhs during 2003-2004. | 2007 | 17963333 |
| protein fog is a moderate inducer of mig/cxcl9, and group g streptococci are more tolerant than group a streptococci to this chemokine's antibacterial effect. | streptococcus dysgalactiae subsp. equisimilis (group g streptococci; ggs) cause disease in humans but are often regarded as commensals in comparison with streptococcus pyogenes (group a streptococci; gas). the current study investigated the degree and kinetics of the innate immune response elicited by the two species. this was assessed as expression of the chemokine mig/cxcl9 and bacterial susceptibility to its bactericidal effect. no significant difference in mig/cxcl9 expression from thp-1 or ... | 2007 | 17975089 |
| cloning and characterization of promoter-active dna sequences from streptococcus equisimilis. | genomic fragments of streptococcus equisimilis exhibiting potent promoter activity in escherichia coli were isolated by transcriptional fusion to chloramphenicol acetyl transferase (cat) structural gene. random s. equanimities dna, cloned in e. coli, exhibited a higher frequency of strong promoter activity than did similarly cloned e. coli fragments. the determination of the relative promoter strength of randomly selected clones with cat assay demonstrated the dominance of sequences acting as a ... | 2007 | 17171467 |
| lateral transfer of alpha-like protein gene cassettes among streptococci: identification of a new family member in streptococcus dysgalactiae subsp. equisimilis. | 2007 | 17257265 | |
| phage 3396 from a streptococcus dysgalactiae subsp. equisimilis pathovar may have its origins in streptococcus pyogenes. | streptococcus dysgalactiae subsp. equisimilis strains (group g streptococcus [ggs]) are largely defined as commensal organisms, which are closely related to the well-defined human pathogen, the group a streptococcus (gas). while lateral gene transfers are emerging as a common theme in these species, little is known about the mechanisms and role of these transfers and their effect on the population structure of streptococci in nature. it is now becoming evident that bacteriophages are major contr ... | 2007 | 17259318 |
| [a case of bacterial pneumonia caused by streptococcus dysgalactiae subsp. equisimilis, showing patchy consolidations resembling organizing pneumonia]. | in december, 2001, a 67-year-old woman was given a diagnosis of having systemic sclerosis and organizing pneumonia. steroid treatment improved her condition, and she received no further medication for approximately three years thereafter. in october 2005, she visited kurume university hospital because of cough and fever. chest x-ray film and high-resolution computed tomography (hrct) showed bilateral patchy consolidation with air-bronchogram sign and ground-glass opacities, predominantly in the ... | 2007 | 17313025 |
| clinical and epidemiological investigation of chronic upper respiratory diseases caused by beta-haemolytic streptococci in horses. | an outbreak of strangle-like disease involving 26 horses farmed in central italy was investigated by clinic examination, endoscopy, cytology, bacteriology and polymerase chain reaction (pcr). at weekly interval, a total of three nasal swabs and one guttural pouches lavage fluid (gplf) were collected, and no streptococcus equi subsp. equi carrier was found. some horses showed upper airways disease and endoscopic signs of pharyngeal lymphoid hyperplasia of different grade and/or abnormal endoscopi ... | 2007 | 17433441 |
| recombinant production of streptococcus equisimilis streptokinase by streptomyces lividans. | streptokinase (sk) is a potent plasminogen activator with widespread clinical use as a thrombolytic agent. it is naturally secreted by several strains of beta-haemolytic streptococci. the low yields obtained in sk production, lack of developed gene transfer methodology and the pathogenesis of its natural host have been the principal reasons to search for a recombinant source for this important therapeutic protein. we report here the expression and secretion of sk by the gram-positive bacterium s ... | 2007 | 17610745 |
| an enzyme capture assay for analysis of active hyaluronan synthases. | we describe a sensitive assay for detection of active hyaluronan synthases (hass) capable of synthesizing hyaluronan (ha) without use of radioactive uridine 5'-diphosphate sugar precursors. the has capture assay is based on the binding of a biotinylated ha binding protein (bhabp) to ha chains that are associated with has and the subsequent capture of bhabp-ha-has complexes with streptavidin-agarose. specific has proteins (e.g., has1, not has2 or has3) captured in this pull-down approach are read ... | 2007 | 17904513 |
| a charge reversal differentiates (p)ppgpp synthesis by monofunctional and bifunctional rel proteins. | a major regulatory mechanism evolved by microorganisms to combat stress is the regulation mediated by (p)ppgpp (the stringent response molecule), synthesized and hydrolyzed by rel proteins. these are divided into bifunctional and monofunctional proteins based on the presence or absence of the hydrolysis activity. although these proteins require mg(2+) for (p)ppgpp synthesis, high mg(2+) was shown to inhibit this reaction in bifunctional rel proteins from mycobacterium tuberculosis and streptococ ... | 2007 | 17911108 |
| detection of equine herpesvirus type 1 using a real-time polymerase chain reaction. | equid herpesvirus 1 (ehv1) is a major disease of equids worldwide causing considerable losses to the horse industry. a variety of techniques, including pcr have been used to diagnose ehv1. some of these pcrs were used in combination with other techniques such as restriction enzyme analysis (rea) or hybridisation, making them cumbersome for routine diagnostic testing and increasing the chances of cross-contamination. furthermore, they involve the use of suspected carcinogens such as ethidium brom ... | 2006 | 16137772 |
| size exclusion chromatography-multiangle laser light scattering analysis of hyaluronan size distributions made by membrane-bound hyaluronan synthase. | size exclusion chromatography-multiangle laser light scattering (sec-malls) analyses of escherichia coli membranes expressing streptococcus equisimilis hyaluronan synthase (sehas) demonstrated an inherent artifact (10-100 mda) that coeluted with hyaluronan (ha) and skewed the apparent weight-average mass of ha to erroneously high values. briefly heating samples to 65-75 degrees c eliminated this artifact and increased the yield of recovered ha due to the release of ha chains that were attached t ... | 2006 | 16476403 |
| mutation of two intramembrane polar residues conserved within the hyaluronan synthase family alters hyaluronan product size. | we identified two conserved polar amino acids within different membrane domains (md) of streptococcus equisimilis hyaluronan synthase (sehas), lys48 in md2 and glu327 in md4. in eukaryotic hass, the position of the glu is very similar and the lys is replaced by a conserved polar gln. to assess whether lys48 and glu327 interact or influence sehas activity, we investigated the effects of changing lys48 to arg or glu and glu327 to lys, asp, or gln. mutants, including a double switch variant with ly ... | 2006 | 16505475 |
| clonal relationships between invasive and noninvasive lancefield group c and g streptococci and emm-specific differences in invasiveness. | lancefield group g and group c streptococci (ggs and gcs, respectively) are pathogens responsible for a number of life-threatening infections. a collection of 116 recent (1998 to 2004) invasive (n = 28) and noninvasive (n = 88) ggs and gcs clinical isolates from portugal were characterized. all isolates were identified as streptococcus dysgalactiae subsp. equisimilis and characterized by emm typing and dna macrorestriction profiling using pulsed-field gel electrophoresis (pfge). emm typing revea ... | 2006 | 16517864 |
| rapid differentiation between members of the anginosus group and streptococcus dysgalactiae subsp. equisimilis within beta-hemolytic group c and g streptococci by pcr. | based on a pair of primers developed initially for differentiating the anginosus group from other viridans streptococci, the pcr reported here can also differentiate between members of the anginosus group and streptococcus dysgalactiae subsp. equisimilis among beta-hemolytic group c and g streptococci. the resulting 742-bp pcr product was specific for members of the anginosus group, although a smaller, nonspecific product (361 bp) was generated from s. dysgalactiae subsp. equisimilis. restrictio ... | 2006 | 16672416 |
| inhibitory effects of triterpenes and flavonoids on the enzymatic activity of hyaluronic acid-splitting enzymes. | the effect of triterpenes and flavonoids on the activity of several hyaluronic acid-splitting enzymes was investigated. studies showed that the inhibitory effect of the triterpenes glycyrrhizin and glycyrrhetinic acid is dependent on the source of hyaluronate lyase. hyaluronate lyase from streptococcus agalactiae (hyal b) and recombinant hyaluronate lyase from streptococcus agalactiae (rhyal b) demonstrated strongest inhibition. in contrast, hyaluronate lyases from streptomyces hyalurolyticus (h ... | 2006 | 16718670 |
| acute peritonitis and salpingitis associated with streptococcal toxic shock syndrome caused by lancefield group g alpha-haemolytic streptococcus dysgalactiae subsp. equisimilis. | the authors treated a patient for what appears to be the first reported occurrence of acute peritonitis and salpingitis associated with streptococcal toxic shock syndrome (stss). this was caused by lancefield group g alpha-haemolytic streptococcus dysgalactiae subsp. equisimilis tkch2004-001. the isolate showed m protein type stc36 and carried the spegg gene. to the best of the authors' knowledge, the present report represents the first case of stss complicating acute peritonitis and salpingitis ... | 2006 | 16772425 |
| dysgalacticin: a novel, plasmid-encoded antimicrobial protein (bacteriocin) produced by streptococcus dysgalactiae subsp. equisimilis. | dysgalacticin is a novel bacteriocin produced by streptococcus dysgalactiae subsp. equisimilis strain w2580 that has a narrow spectrum of antimicrobial activity directed primarily against the principal human streptococcal pathogen streptococcus pyogenes. unlike many previously described bacteriocins of gram-positive bacteria, dysgalacticin is a heat-labile 21.5 kda anionic protein that kills its target without inducing lysis. the n-terminal amino acid sequence of dysgalacticin [asn-glu-thr-asn-a ... | 2006 | 16804174 |
| [use of hyaluronic acid cleaving enzymes for absorption acceleration. results of an in vitro study with xylazine and ketamine]. | the so-called "hellabrunner mischung", (combination of xylazine and ketamine with hyaluronidase) is frequently used for the immobilisation of wildlife animals. the enzyme hyaluronidase shall improve the distribution of the intramuscularly or subcutaneously administered compounds in the tissue and enhance their absorption. these enhancing effects of two hyaluronate lyases of bacterial origin (streptococcus agalactiae and streptococcus equisimilis) and a testicular hyaluronidase were compared in a ... | 2006 | 16892706 |
| [a case of severe necrotizing cellulitis caused by group g streptococcus dysgalactiae subsp. equisimilis]. | group g streptococcus (ggs) is infrequently associated with severe invasive soft tissue infection and toxic shock syndrome. a 74-year-old woman with a history of lymphedema of the lower extremities after surgical and radiation therapy for uterine cancer and diabetic mellitus and admitted for swelling of the right leg, fever, and dyspnea. she presented with shock and necrotizing cellulitis of the right lower extremity. laboratory tests showed leukocytepenia, acute renal and liver dysfunction, and ... | 2006 | 16922490 |
| dna methylase activity as a marker for the presence of a family of phage-like elements conferring efflux-mediated macrolide resistance in streptococci. | recently, two related chimeric genetic elements (tn1207.3 and phi10394.4) were shown to carry the macrolide efflux gene mef in streptococcus pyogenes (group a streptococci [gas]). the dissemination of elements belonging to the tn1207.3/phi10394.4 family in recent isolates of gas, streptococcus dysgalactiae subsp. equisimilis, streptococcus pneumoniae, and streptococcus agalactiae recovered in portugal was surveyed. in total, 149 gas, 18 s. pneumoniae, 4 s. dysgalactiae subsp. equisimilis, and 5 ... | 2006 | 16954322 |
| phospholipid dependence and liposome reconstitution of purified hyaluronan synthase. | previous radiation inactivation and enzyme characterization studies demonstrated that the streptococcus equisimilis hyaluronan synthase (sehas) is phospholipid-dependent and that cardiolipin (cl) is the best phospholipid for enzyme activation. here we investigated the ability of sehas, purified in the absence of added lipid, to be activated by synthetic phosphatidic acid (pa), phosphatidylserine, or cl lipids containing fatty acyl chains of different length or different numbers of double bonds. ... | 2006 | 16984914 |
| [emm typing by genetic identification of streptococcus dysgalactiae subsp. equisimilis and susceptibility to oral antibiotics]. | a total of 593 beta-hemolytic streptococci belonging to lancefield group a (gas), group c (gcs) or group g (ggs) according to agglutination tests were collected from 11 medical institutions between september 2003 and october 2005. in total, 128 strains were identified as streptococcus dysgalactiae subsp. equisimilis (s. equisimilis) using physiological tests. of these strains, 5 strains were agglutinated to lancefield group a, 17 strains to group c, and 106 strains to group g. most of these stra ... | 2006 | 17073261 |
| [a case of thoracic empyema with gas formation associated with streptococcus dysgalactiae subsp. equisimilis]. | a 63-year-old woman suffering from dyspnea since 1 week earlier underwent a combination therapy of oral corticosteroid and ciclosporin for rheumatic arthritis. chest radiography showed plural effusion with gas formation in the right thorax. empyema was diagnosed based on the specimen from pleural effusion. after a chest tube was emplaced to remove and wash out pus, antibiotics were started and empyema improved immediately. streptococcus dysgalactiae subsp. equisimilis, but not aerobic bacilli, w ... | 2006 | 17073267 |
| three cases of vertebral osteomyelitis caused by streptococcus dysgalactiae subsp. equisimilis. | three cases of vertebral osteomyelitis caused by streptococcus dysgalactiae subsp. equisimilis (strep. equisimilis) are presented here. all three cases presented with fever, back pain, general malaise and weight loss for at least 4 weeks. diagnosis was established by culture of a spinal biopsy and/or positive blood cultures together with radiological findings. in all three cases, 6-12 weeks of antibiotics were curative without recourse to surgery. the ability of strep. equisimilis to cause verte ... | 2005 | 16192443 |
| hyaluronic acid production in bacillus subtilis. | the hasa gene from streptococcus equisimilis, which encodes the enzyme hyaluronan synthase, has been expressed in bacillus subtilis, resulting in the production of hyaluronic acid (ha) in the 1-mda range. artificial operons were assembled and tested, all of which contain the hasa gene along with one or more genes encoding enzymes involved in the synthesis of the udp-precursor sugars that are required for ha synthesis. it was determined that the production of udp-glucuronic acid is limiting in b. ... | 2005 | 16000785 |
| hyaluronan biosynthesis by class i streptococcal hyaluronan synthases occurs at the reducing end. | previous studies reached different conclusions about whether class i hyaluronan synthases (hass) elongate hyaluronic acid (ha) by addition to the reducing or the nonreducing end. here we used two strategies to determine the direction of ha synthesis by purified class i hass from streptococcus equisimilis and streptococcus pyogenes. in the first strategy we used each of the two udp-sugar substrates separately to pulse label either the beginning or the end of ha chains. we then quantified the rela ... | 2005 | 15668242 |
| six-month multicenter study on invasive infections due to streptococcus pyogenes and streptococcus dysgalactiae subsp. equisimilis in argentina. | during a 6-month period, 95 invasive infections due to streptococcus pyogenes and group c or group g streptococcus dysgalactiae subsp. equisimilis were recorded from 40 centers of 16 cities in argentina. we describe here epidemiologic data available for 55 and 19 patients, respectively, associated with invasive infections due to s. pyogenes and s. dysgalactiae subsp. equisimilis. the associated isolates and 58 additional pharyngeal isolates were genotyped and subjected to serologic and/or antibi ... | 2005 | 15695683 |
| inter-species genetic movement may blur the epidemiology of streptococcal diseases in endemic regions. | streptococcus dysgalactiae subsp. equisimilis (human group g streptococcus, ggs) is generally regarded as a commensal organism but can cause a spectrum of human diseases very similar to that caused by s. pyogenes (group a streptococcus, gas). lateral acquisition of genes between these two phylogenetically closely related species is well documented. however, the extent and mechanisms of lateral acquisitions is not known. we report here genomic subtraction between a pathogenic ggs isolate and a co ... | 2005 | 15893492 |
| group c streptococcal bacteremia: a case report from india. | group c streptococci are a common cause of infection in animals and a rare cause of bacteremia in human beings. the entity is often seen in elderly people with a severe underlying illness. we report here the only case of group c streptococcal bacteremia reported in our hospital, caused by streptococcus equisimilis, a beta-hemolytic group c streptococcus. the patient was a 10-year old male with a known history of aplastic anemia. in spite of specific therapy with penicillin, the outcome was fatal ... | 2004 | 15916084 |
| acquisition of host plasmin activity by the swine pathogen streptococcus suis serotype 2. | in this study, the plasminogen-binding activity of streptococcus suis serotype 2 was investigated. bound human plasminogen was activated by purified streptokinase, urokinase, or streptococcus dysgalactiae subsp. equisimilis culture supernatant. both human and porcine plasminogen were bound by s. suis. binding was inhibited by epsilon-aminocaproic acid, and the plasminogen receptor was heat and sodium dodecyl sulfate resistant. one of the receptors was identified as glyceraldehyde-3-phosphate deh ... | 2004 | 14688145 |
| the role of group c and group g streptococci in acute pharyngitis in children. | group c streptococci (gcs) and group g beta-haemolytic streptococci (ggs) have caused well-documented epidemics of acute pharyngitis in children, but the importance of these organisms in causing endemic or sporadic pharyngitis is uncertain. the heterogeneity of gcs and ggs may obscure the role of certain subtypes, such as the large-colony-forming strains of group c (streptococcus dysgalactiae subsp. equisimilis) or group g, in endemic pharyngitis. for a 1-year period, children (aged 6 months to ... | 2004 | 14706084 |
| characterization of group c and g streptococcal strains that cause streptococcal toxic shock syndrome. | twelve strains (the largest number ever reported) of group c and g(1) streptococci (gcs and ggs, respectively) that caused streptococcal toxic shock syndrome (stss) were collected and characterized. eleven strains were identified as streptococcus dysgalactiae subsp. equisimilis, and one strain was identified as streptococcus equi subsp. zooepidemicus. we found that it was the first reported case of stss caused by s. equi subsp. zooepidemicus. cluster analysis according to the 16s rrna gene (rdna ... | 2004 | 14715751 |
| surveillance of severe invasive group-g streptococcal infections and molecular typing of the isolates in japan. | the number of patients with severe invasive group-g streptococcal (streptococcus dysgalactiae subsp. equisimilis) infections has been increasing in japan. the emm genotypes and smai-digested pulsed-field gel electrophoresis dna profiles were variable among the strains isolated, suggesting there has not been clonal expansion of a specific subpopulation of strains. however, all strains carried scpa, ska, slo and sag genes, some of which may be involved in the pathogenesis of the disease. | 2004 | 14979600 |
| conformational antagonism between opposing active sites in a bifunctional rela/spot homolog modulates (p)ppgpp metabolism during the stringent response [corrected]. | enzymes of the rel/spo family enable bacteria to survive prolonged periods of nutrient limitation by producing an intracellular signaling alarmone, (p)ppgpp, which triggers the so-called stringent response. both the synthesis of (p)ppgpp from atp and gdp(gtp), and its hydrolysis to gdp(gtp) and pyrophosphate, are catalyzed by rel/spo proteins. the 2.1 a crystal structure of the bifunctional catalytic fragment of the rel/spo homolog from streptococcus dysgalactiae subsp. equisimilis, rel(seq), re ... | 2004 | 15066282 |
| characterization of the purified hyaluronan synthase from streptococcus equisimilis. | hyaluronan synthase (has) utilizes udp-glcua and udp-glcnac in the presence of mg(2+) to form the gag hyaluronan (ha). the purified has from streptococcus equisimilis (sehas) shows high fidelity in that it only polymerizes the native substrates, udp-glcnac and udp-glcua. however, other uridinyl nucleotides and udp-sugars inhibited enzyme activity, including udp-galnac, udp-glc, udp-gal, udp-galua, ump, udp, and utp. purified sehas was approximately 40% more active in 25 mm, compared to 50 mm, po ... | 2004 | 15248781 |
| lameness in piglets. abrasions in nursing piglets and transfer of protection towards infections with streptococci from sow to offspring. | a group of 175 newborn piglets were monitored with respect to development of abrasions and lameness. lameness was diagnosed in 10.9% of the piglets. about every second litter was affected and around 75% of these diagnoses took place during the first 3 weeks of life. skin lesions were present already on day 3. they increased in magnitude until day 10 and thereafter declined. they were generally bilateral and most commonly observed as abrasions over the carpal joints. hocks, face and tails were af ... | 2004 | 15458490 |
| [evaluation of prolex for the rapid identification of streptococci isolated in medical microbiology]. | the need to rapidly identify streptococci responsible for acute infectious diseases has led to the development of agglutination techniques that are able to identify streptococcal group antigens (a, b, c, d, f, and g) directly from primoculture colonies on blood agar. the prolex agglutination tests (pro-lab diagnostics, richmond hill, ontario, canada), distributed in france by i2a, have been used for the determination of group antigens of 166 isolates of streptococci and enterococci previously id ... | 2004 | 15465260 |
| identification of a membrane-localized cysteine cluster near the substrate-binding sites of the streptococcus equisimilis hyaluronan synthase. | the membrane-bound hyaluronan synthase (has) from streptococcus equisimilis (sehas), which is the smallest class i has, has four cysteine residues (positions 226, 262, 281, and 367) that are generally conserved within this family. although cys-null sehas is still active, chemical modification of cysteine residues causes inhibition of wild-type enzyme. here we studied the effects of n-ethylmaleimide (nem) treatment on a panel of sehas cys-mutants to examine the structural and functional roles of ... | 2004 | 15616126 |
| analysis of a viridans group strain reveals a case of bacteremia due to lancefield group g alpha-hemolytic streptococcus dysgalactiae subsp equisimilis in a patient with pyomyositis and reactive arthritis. | streptococcus dysgalactiae is classified by a combination of phenotypic and genotypic characteristics into lancefield group c alpha-hemolytic streptococcus dysgalactiae subsp. dysgalactiae and lancefield group c, group g, and group l beta-hemolytic streptococcus dysgalactiae subsp. equisimilis. in this study, we report the isolation of a catalase-negative, alpha-hemolytic, optochin- and bacitracin-resistant viridans group strain, which does not grow in 10 or 40% bile, on macconkey agar or bile e ... | 2003 | 12574255 |
| acute epididymitis and septicemia due to streptococcus equisimilis. | 2003 | 12798224 | |
| rdna sequence analyses of streptococcus dysgalactiae subsp. equisimilis isolates from pigs. | the nucleotide sequences of 16s and 23s rrna genes (rdna) were determined for 11 isolates of streptococcus dysgalactiae subsp. equisimilis from slaughtered pigs with endocarditis, arthritis or lymphadenitis and strain atcc 35666, designated as a strain of subspecies equisimilis. the sequences of each of the genes were compared phylogenetically with the corresponding sequences of s. dysgalactiae subsp. dysgalactiae atcc 43078(t) and atcc 27957, which were also determined in this study. based on t ... | 2003 | 14657127 |
| specificity role of the streptokinase c-terminal domain in plasminogen activation. | several pathogenic bacteria secrete plasminogen activator proteins. streptokinase (ske) produced by streptococcus equisimilis and staphylokinase secreted from staphylococcus aureus are human plasminogen activators and streptokinase (sku), produced by streptococcus uberis, is a bovine plasminogen activator. thus, the fusion proteins among these activators can explain the function of each domain of ske. replacement of the skalpha domain with staphylokinase donated the staphylokinase-like activatio ... | 2002 | 11779212 |
| the streptococcal hyaluronan synthases are inhibited by sulfhydryl-modifying reagents, but conserved cysteine residues are not essential for enzyme function. | hyaluronan (ha) synthase (has) is a membrane-bound enzyme that utilizes udp-glucuronic acid (glcua) and udp-glcnac to synthesize ha. the has from streptococcus pyogenes (sphas, 419 amino acids) contains six cys residues, whereas the enzyme from streptococcus equisimilis (sehas, 417 amino acids) contains four cys residues. these cys residues of sehas are highly conserved in all class i has family members. here we investigated the structural and functional roles of these conserved cysteines in seh ... | 2002 | 11799120 |
| [streptococcus dysgalactiae subsp. equisimilis possessing lancefield's group a antigen]. | we isolated streptococcus dysgalactiae subsp. equisimilis with lancefield's group a antigen from a patient in osaka city. this clinical isolate formed beta-hemolytic large colonies on sheep blood agar, and was catalase-negative gram-positive cocci carrying lancefield's group a antigen. therefore, streptococcus pyogenes and this clinical isolate could not be classified by the conventional method on the basis of lancefield's group antigen. in addition, the rapid detection system of group a strepto ... | 2002 | 11974882 |
| intramolecular regulation of the opposing (p)ppgpp catalytic activities of rel(seq), the rel/spo enzyme from streptococcus equisimilis. | catalytic and regulatory domains of the rel/spo homolog of streptococcus equisimilis affecting (p)ppgpp synthesis and degradation activities have been defined, and opposing activities of the purified protein and its fragments have been compared. two major domains of the 739-residue rel(seq) protein are defined by limited proteolytic digestion. in vitro assays of the purified n-terminal half-protein reveal synthesis of (p)ppgpp by an atp-gtp 3'-pyrophosphotransferase as well as an ability to degr ... | 2002 | 12003927 |
| dual control of streptokinase and streptolysin s production by the covrs and fascax two-component regulators in streptococcus dysgalactiae subsp. equisimilis. | synthesis of the plasminogen activator streptokinase (sk) by group a streptococci (gas) has recently been shown to be subject to control by two two-component regulators, covrs (or csrrs) and fasbca. in independent studies, response regulator covr proved to act as the repressor, whereas fasa was found to act indirectly as the activator by controlling the expression of a stimulatory rna, fasx. in an attempt at understanding the regulation of sk production in the human group c streptococcal (gcs) s ... | 2002 | 12065504 |
| unfolding and aggregation during the thermal denaturation of streptokinase. | the thermal denaturation of streptokinase from streptococcus equisimilis (sk) together with that of a set of fragments encompassing each of its three domains has been investigated using differential scanning calorimetry (dsc). analysis of the effects of ph, sample concentration and heating rates on the dsc thermograms has allowed us to find conditions where thermal unfolding occurs unequivocally under equilibrium. under these conditions, ph 7.0 and a sample concentration of less than approximate ... | 2002 | 12180989 |
| streptococcus equisimilis infection in striped skunks (mephitis mephitis) in saskatchewan. | three radio-collared striped skunks (mephitis mephitis) found dead during a field study of winter ecology of striped skunks near willowbrook, saskatchewan, canada were examined. streptococcus equisimilis was identified as the primary agent causing necrotizing purulent pneumonia in one skunk and suppurative meningoencephalitis in another. both streptococcus equisimilis and streptococcus canis were isolated from lesions of purulent myocarditis and pyothorax in the third skunk. these are apparently ... | 2002 | 12238389 |
| [cerebrospinal fluid rhinorrhea and streptococcus equisimilis-related meningitis 16 years after a head injury]. | a case of meningitis caused by streptococcus equisimilis and cerebrospinal fluid rhinorrhea, in which the head trauma occurred 16 years before, is presented. to the best of the author's knowledge this is the first case reported with such characteristics. several precipitating factors could be responsible for the unusually late reopening of the fistula streptococci equisimilis is an uncommon cause of the bacteremia. an appropriate antimicrobrial therapy against s. equisimilis followed by surgical ... | 2002 | 12355655 |
| superantigen-like gene(s) in human pathogenic streptococcus dysgalactiae, subsp equisimilis: genomic localisation of the gene encoding streptococcal pyrogenic exotoxin g (speg(dys)). | streptococcus pyogenes (gas) causes about 90% of streptococcal human infections while group c (gcs) and g (ggs) streptococci can be pathogenic for different mammalians. especially the human pathogenic gcs and ggs, streptococcus dysgalactiae, subsp. equisimilis, account for 5-8% of the human streptococcal diseases like wound infections, otitis media, purulent pharyngitis and also streptococcal toxic shock syndrome. a defined superantigen so far was not identified in gcs and ggs strains. in the pr ... | 2002 | 12381468 |
| acute bacterial arthritis caused by group c streptococci. | to summarize the characteristics of and clinical experience with joint infection by group c streptococcus. | 2001 | 11503138 |
| [bacteremic pneumonia due to streptococcus equisimilis]. | 2001 | 11562324 | |
| a definite case of spondylodiscitis caused by streptococcus equisimilis. | to shed light on the role of streptococcus equisimilis (se) in the pathogenesis of intervertebral disc infection, we report here a case of lumbar spondylodiscitis in a 37-year-old male caused by se, with identification of this strain by cultures from l4-l5 lumbar disc biopsy. intravenous therapy with penicillin and gentamycin combined with immobilization resulted in a rapid and complete recovery. the patient did not have underlying disease and showed no obvious history of exposure to animals. we ... | 2001 | 11579722 |
| application of in situ pcr to diagnose pneumonia in medico-legal autopsy cases. | in situ polymerase chain reaction (in situ pcr) can detect specific sequences of dna, such as those of micro-organisms in human tissue samples. in forensic medicine, there are many cases implicated with infection, and pneumonia is an especially common finding in autopsy cases. in the present study, we tried to detect the presence of bacterial infections in lung tissue samples. the experiment was performed with ten paraffin-embedded lung tissue samples, including three non-pneumonia cases using s ... | 2001 | 12935517 |
| allele substitution of the streptokinase gene reduces the nephritogenic capacity of group a streptococcal strain nz131. | to investigate the role of allelic variants of streptokinase in the pathogenesis of acute poststreptococcal glomerulonephritis (apsgn), site-specific integration plasmids were constructed, which contained either the non-nephritis-associated streptokinase gene (skc5) from the group c streptococcal strain streptococcus equisimilis h46a or the nephritis-associated streptokinase gene (ska1) from the group a streptococcal nephritogenic strain nz131. the plasmids were introduced by electroporation and ... | 2000 | 10678902 |
| haemolysin-deficient variants of streptococcus pyogenes and s. dysgalactiae subsp. equisimilis may be overlooked as aetiological agents of pharyngitis. | variants of large colony beta-haemolytic lancefield group a, c and g streptococci that are non-haemolytic or alpha-haemolytic on sheep blood agar have been detected in clinical specimens due to their enhanced haemolytic activity when grown on a new selective and differential blood agar medium containing colistin, nalidixic acid and ph 7.5-adjusted pipes buffer (cna-p). the large colony lancefield group c and g isolates were identified as streptococcus dysgalactiae subsp. equisimilis by api 20 st ... | 2000 | 10966229 |
| [infective endocarditis in intravenous drug users. study of 34 cases]. | infective endocarditis (ie) in injection drug users (idu) have a special etiologic, epidemiological and clinical characteristics different to ie in the general population. the clinical and evolutive features of a group of ie in ivd are reported. | 2000 | 10981332 |
| characterization of acid phosphatase activities in the equine pathogen streptococcus equi. | acid phosphatases hydrolyse phosphomonoesters at acidic ph in a variety of physiological contexts. the recently defined class c family of acid phosphatases includes the 32 kda lppc lipoprotein of streptococcus equisimilis. to define further the distribution of acid phosphatases in the genus streptococcus we have examined the equine pathogens streptococcus equi subsp. equi and streptococcus equi subsp. zooepidemicus. whole cell assays indicated that these organisms possess two acid phosphatases w ... | 2000 | 11108009 |
| purification and lipid dependence of the recombinant hyaluronan synthases from streptococcus pyogenes and streptococcus equisimilis. | the two hyaluronan synthases (hass) from streptococcus pyogenes (sphas) and streptococcus equisimilis (sehas) were expressed in escherichia coli as recombinant proteins containing his6 tails. both enzymes were expressed as major membrane proteins, accounting for approximately 5-8% of the total membrane protein. using nickel chelate affinity chromatography, the hass were purified to homogeneity from n-dodecyl beta-d-maltoside extracts. high levels of has activity could be achieved only if the pur ... | 1999 | 9933623 |
| kinetic characterization of the recombinant hyaluronan synthases from streptococcus pyogenes and streptococcus equisimilis. | the two hyaluronan synthases (hass) from streptococcus pyogenes (sphas) and streptococcus equisimilis (sehas) were expressed in escherichia coli as recombinant proteins containing his6 tails. the accompanying paper has described the purification and lipid dependence of both hass, their preference for cardiolipin, and their stability during storage (tlapak-simmons, v. l., baggenstoss, b. a., clyne, t., and weigel, p. h. (1999) j. biol. chem. 274, 4239-4245). kinetic characterization of the enzyme ... | 1999 | 9933624 |
| purification and cloning of a streptokinase from streptococcus uberis. | a bovine plasminogen activator was purified from the culture supernatant of the bovine pathogen streptococcus uberis nctc 3858. after the final reverse-phase high-performance liquid chromatography step a single protein with a molecular mass of 32 kda was detected in the active fraction. a partial peptide map was established, and degenerate primers were designed and used for amplification of fragments of the gene encoding the activator. inverse pcr was subsequently used for obtaining the full-len ... | 1999 | 10024545 |
| [septic arthritis by streptococcus equisimilis in a patient allergic to beta-lactam antibiotics and aminoglycosides]. | 1999 | 10563096 | |
| characterization of blood culture isolates of streptococcus dysgalactiae subsp. equisimilis possessing lancefield's group a antigen. | for three human blood culture isolates of beta-hemolytic streptococci with lancefield's serogroup a antigen, phylogenetic analysis of the 16s rrna genes confirmed biochemical identification as streptococcus dysgalactiae subsp. equisimilis. genes encoding m or m-like proteins, which are considered to be major virulence determinants in streptococci, were detected in all of these strains. our data clearly demonstrate that for beta-hemolytic streptococci, the species assignment should not be based o ... | 1999 | 10565964 |
| cloning, expression, sequence analysis, and characterization of streptokinases secreted by porcine and equine isolates of streptococcus equisimilis. | streptokinases secreted by nonhuman isolates of group c streptococci (streptococcus equi, s. equisimilis, and s. zooepidemicus) have been shown to bind to different mammalian plasminogens but exhibit preferential plasminogen activity. the streptokinase genes from s. equisimilis strains which activated either equine or porcine plasminogen were cloned, sequenced, and expressed in escherichia coli. the streptokinase secreted by the equine isolate had little similarity to any known streptokinases se ... | 1999 | 10569766 |
| species specificity of plasminogen activation and acquisition of surface-associated proteolytic activity by group c streptococci grown in plasma. | our laboratory previously demonstrated that group c streptococcal isolates from humans and horses secrete streptokinases that preferentially activate plasminogens reflecting the origin of the isolates. to analyze the significance of these findings, series of streptokinase-producing streptococcus equisimilis isolates recovered from humans and horses were examined. southern blot analysis revealed that chromosomal dna of the streptococcal isolates from humans reacted exclusively with a skc(hu) prob ... | 1999 | 10569767 |
| effects of synthetic hydroxy isothiocyanates on microbial systems. | hydroxy isothiocyanates (itcs), including some new derivatives of naturally occurring compounds, were synthesized and their minimum inhibitory, minimum fungicidal, and minimum bactericidal concentrations for aspergillus niger, aspergillus fumigatus, staphylococcus aureus, and escherichia coli were estimated. these compounds were strongly antimicrobial; for example, 2-(4-hydroxyphenyl) ethyl itc inhibited growth of all strains examined at concentrations of 7.8 to 15.6 micrograms/ml. the atp conce ... | 1998 | 9571778 |
| a new case of streptococcus equisimilis septic arthritis. | 1998 | 9586685 | |
| cytoplasmic membrane lipoprotein lppc of streptococcus equisimilis functions as an acid phosphatase. | the function of the streptococcal cytoplasmic membrane lipoprotein, lppc, was identified with isogenic streptococcus equisimilis h46a and escherichia coli jm109 strain pairs differing in whether they contained [h46a and jm109(plpp2)] or lacked (h46a lppc::plpp10 and jm109) the functional lppc gene for comparative phosphatase determinations under acidic conditions. lppc-directed acid phosphatase activity was demonstrated zymographically and by specific enzymatic activity assays, with whole cells ... | 1998 | 9647812 |
| effect of signal peptide changes on the extracellular processing of streptokinase from escherichia coli: requirement for secondary structure at the cleavage junction. | streptokinase (sk), an extracellular protein from streptococcus equisimilis, is secreted post-translationally by escherichia coli using both its native and e. coli-derived transport signals. in this communication we report that cleavage specificity of signal peptidase i, and thus efficiency of secretion, varies in e. coli when sk export is directed by different transport signals. the native (+1) n-terminus of mature sk was retained when it was transported under the control of its own, pelb or la ... | 1998 | 9648736 |
| the active streptococcal hyaluronan synthases (hass) contain a single has monomer and multiple cardiolipin molecules. | the functional sizes of the two streptococcal hyaluronan synthases (hass) were determined by radiation inactivation analysis of isolated membranes. the native enzymes in membranes from group a streptococcus pyogenes has and group c streptococcus equisimilis has were compared with the recombinant proteins expressed in escherichia coli membranes. based on their amino acid sequences, the masses of these four proteins as monomers are approximately 48 kda. in all cases, loss of enzyme activity was a ... | 1998 | 9748290 |
| spondylodiscitis caused by streptococcus equisimilis. | 1998 | 9783775 | |
| cloning, expression and purification of recombinant streptokinase: partial characterization of the protein expressed in escherichia coli. | we cloned the streptokinase (stk) gene of streptococcus equisimilis in an expression vector of escherichia coli to overexpress the profibrinolytic protein under the control of a tac promoter. almost all the recombinant stk was exported to the periplasmic space and recovered after gentle lysozyme digestion of induced cells. the periplasmic fraction was chromatographed on deae sepharose followed by chromatography on phenyl-agarose. active proteins eluted between 4.5 and 0% ammonium sulfate, when a ... | 1997 | 9686161 |
| characterization of the stringent and relaxed responses of streptococcus equisimilis. | the 739-codon rel(seq) gene of streptococcus equisimilis h46a is bifunctional, encoding a strong guanosine 3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (ppgppase) and a weaker ribosome-independent atp:gtp 3'-pyrophosphoryltransferase [(p)ppgpp synthetase]. to analyze the function of this gene, (p)ppgpp accumulation patterns as well as protein and rna synthesis were compared during amino acid deprivation and glucose exhaustion between the wild type and an insertion mutant carrying a rel(seq) ge ... | 1997 | 9098065 |
| differentiation of human and animal strains of streptococcus dysgalactiae by pulsed-field gel electrophoresis. | the genetic diversity among 54 human isolates and 33 animal isolates belonging to the species streptococcus dysgalactiae (20 alpha-haemolytic streptococcus dysgalactiae, 23 streptococcus equisimilis, 43 group g streptococci and one group l streptococcus) was evaluated by macrorestriction analysis of chromosomal dna with smai and resolution by pulsed-field gel electrophoresis. this technique revealed a high degree of intraspecies polymorphism, leading to the differentiation of 80 distinct banding ... | 1997 | 9163914 |
| pyogenic arthritis caused by streptococcus equisimilis (group-c streptococcus) in a patient with aids. | a patient with the acquired immunodeficiency syndrome (aids) treated with a daily low dose of corticosteroids for chronic atopic dermatitis experienced a sudden episode of unilateral knee arthritis. culture of the purulent synovial liquid yielded a pure culture of streptococcus equisimilis. a four week period of intravenous antibiotherapy combined with repeated drainages allowed a complete recovery of articular function. | 1997 | 9184272 |
| the lppc gene of streptococcus equisimilis encodes a lipoprotein that is homologous to the e (p4) outer membrane protein from haemophilus influenzae. | we report the cloning, sequencing, and analysis of a novel chromosomal gene of streptococcus equisimilis strain h46a that codes for a membrane lipoprotein, designated lppc. the lppc gene is located 3' adjacent to, and co-oriented with, the unrelated gapc gene that encodes the previously characterized glyceraldehyde-3-phosphate dehydrogenase. sequencing of lppc revealed an 855-bp open reading frame that predicted a 32.4-kda polypeptide possessing a potential lipoprotein signal sequence and modifi ... | 1997 | 9255768 |
| analysis of a case of recurrent bacteraemia due to group a streptococcus equisimilis by pulsed-field gel electrophoresis. | an 86-year-old woman with a history of metastatic breast cancer developed two episodes of streptococcal bacteraemia and erysipelas separated by an interval of 3 months. the isolates belonged to lancefield group a but were biochemically identified as streptococcus equisimilis. the similarity of the two isolates was established by dna macrorestriction analysis and pulsed-field gel electrophoresis, indicating that the second episode was due to relapse and not reinfection. | 1997 | 9266266 |
| catheter-related streptococcus equisimilis bacteremia in a four-month-old infant with congenital cytomegalovirus infection. | 1997 | 9306491 | |
| primary structure requirements for in vivo activity and bidirectional function of the transcription terminator shared by the oppositely oriented skc/rel-orf1 genes of streptococcus equisimilis h46a. | the region between the streptococcus equisimilis streptokinase (skc) gene and the oppositely oriented rel-orf1 transcription unit contains only one termination site known to function bidirectionally in both the homologous host and in escherichia coli. the terminator sequence is similar to other factor-independent terminators. using two sets of point mutations that interrupt the hairpin-upstream oligo(da) tract or the hairpin-downstream oligo(dt) tract, we examined the possible contribution of ex ... | 1997 | 9323365 |