Publications

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spatiotemporal transfer of carbon-14-labelled photosynthate from ectomycorrhizal pinus densiflora seedlings to extraradical mycelia.seedlings of pinus densiflora colonized by an unidentified ectomycorrhizal fungus (t01) were labelled photosynthetically with 14c. movement of 14c-labelled photosynthates within the underground part of the seedlings was investigated by temporal autoradiography using an imaging plate. within 1 day, 14c was transferred from the shoot to the underground part that included roots, mycorrhizae, and the extraradical mycelium; within 3 days, the 14c in the underground part reached its maximum density. m ...200212035731
considerations of correlated fertility between genders on genetic diversity: the pinus densiflora seed orchard as a model.the correlation between 99 clone female and male fertilities in a first generation seed orchard of pinus densiflora was studied over 6 years. the effective number of the parent ( n(p)) and the variance effective population number [ n(e)((v))] were used to assess the impact of total (psi(t)), female (psi(f)) and male (psi(m)) fertility variation. a theoretical framework was developed to account for female and male fertility correlations as well as the impact of possible pollen contamination. tota ...200212582897
measurement of ethylene emission from japanese red pine (pinus densiflora) under field conditions in nox-polluted areas.emission of ethylene from the needles of japanese red pine, pinus densiflora, was measured in air-polluted areas in hiroshima, japan. we applied a suitable protocol to determine the rate of ethylene emission from the excised needles. the influence of excision of needles on ethylene emission was not detected during the first 4 h of incubation at 20 degrees c. ethylene emissions were low in the unpolluted (clean) areas regardless of the altitude or season. the emission of stress ethylene increased ...200111202743
the anxiolytic effect of sho-ju-sen, a japanese herbal medicine, assessed by an elevated plus-maze test in mice.sho-ju-sen (sk), a japanese herbal medicine with a nourishing tonic action, is composed of a water extract of kumazasa leaves (sasa kurinensis makino et sibata) (ss), and ethanol extracts of japanese red pine needles (pinus densiflora sieb. et zucc) (pn) and ginseng roots (panax ginseng c. a. meyer) (px) in the ratio 8:1:1. in this study, an elevated plus-maze test in mice was carried out to assess whether sk had an anxiolytic effect. no significant change was observed in either the plus-maze or ...200111268115
outcrossing and paternity analysis of pinus densiflora (japanese red pine) by microsatellite polymorphism.this study employed microsatellite loci to analyse outcrossing rate and pollen dispersal in japanese red pine (pinus densiflora) in an isolated stand. the average offspring outcrossing rate for 29 cones was 0.955. significant differences in outcrossing rates between offspring groups on individual branches that extended in different directions at different heights were not detected. male parents of 874 offspring collected from the maternal tree were assessed by exclusion using polymorphisms at th ...200111678991
cloning of dna sequences localized on proximal fluorescent chromosome bands by microdissection in pinus densiflora sieb. & zucc.japanese red pine, pinus densiflora, has 2n=24 chromosomes, of which most carry chromomycin a3 (cma) and 4',6-diamidino-2-phenylindole (dapi) bands at their centromere-proximal regions. it was proposed that these regions contain highly repetitive dna. the dna localized in the proximal fluorescent bands was isolated and characterized. in p. densiflora, centromeric and neighboring segments of the somatic chromosomes were dissected with a manual micromanipulator. the centromeric dna was amplified f ...200111685534
a new c-methylated flavonoid glycoside from pinus densiflora.a new c-methyl flavonol glycoside, 5,7,8,4'-tetrahydroxy-3-methoxy-6-methylflavone 8-o-beta-d-glucopyranoside (1), has been isolated from the needles of pinus densiflora, together with kaempferol 3-o-beta-(6"-acetyl)-galactopyranoside.200111731124
[individual biomass of natural pinus densiflora].the aboveground biomass of individuals with different growth potentials in natural pinus densiflora forest with different stand densities was measured in yanbian, jilin province. the variation of individual biomass affected by densities was in order of dominant tree < intermediate tree < suppressed tree, while the distribution proportion of biomass in different organs affected by densities was: in order of trunk > branch > needle > bark. the biomass components of p. densifliora with different gr ...200011766588
isolation and characterization of microsatellite loci from the japanese red pine, pinus densiflora. 200010964246
freezing behaviors in leaf buds of cold-hardy conifers visualized by nmr microscopy.1h-nuclear magnetic resonance (nmr) microscopy was used to study freezing behavior in wintering leaf buds of momi fir (abies firma sieb. et zucc.) and japanese red pine (pinus densiflora sieb. et zucc.). the images acquired predominantly reflected the density of mobile (i.e., non-ice) protons mainly from unfrozen water. by comparing images taken at various subfreezing temperatures, we determined which tissues produced the high and low temperature exotherms detected by differential thermal analys ...199812651356
distribution of cesium-137 in japanese forest soils: correlation with the contents of organic carbon.the spatial and vertical distributions of 137cs in surface soils were surveyed and analyzed then correlated with the contents of organic carbon in the hinoki (chamaecyparis obtusa sieb. et zucc.) plantation forest and secondary forest dominated by red pine (pinus densiflora sieb. et zucc.) in japan. the spatial variation of 137cs activity was observed in the surface soil around the red pine. the average activity of 16 samples around the tree is 42.4 bq/kg and the standard deviation is 25.9 bq/kg ...19989851070
a simple and rapid method for isolation of high quality genomic dna from fruit trees and conifers using pvp.because dna degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality dna from plants containing a high content of polyphenolics has been a difficult problem. we demonstrate an easy extraction process by modifying several existing ones. using this process we have found it possible to isolate dnas from four fruit trees, grape (vitis spp.), apple (malus spp.), pear (pyrus spp.) and persimmon (diospyros spp.) and four species of conifer, pinus de ...19979023124
[studies on the constituents of pinus densiflora sieb. et zucc. leaves. ii. on the ethanol-extractable amino acids compared with pinus thunbergii parl. leaves].in order to identify the constituents of ethanol-extractable amino acids (free amino acids) from pinus densiflora sieb. et zucc. and pinus thunbergii parl. leaves, analytical studies were performed by the use of high performance liquid chromatography. according to the results obtained, 29 amino acids in pinus densiflora (the tree aged 3), 27 amino acids in pinus thunbergii (the tree aged 3) and 25 amino acids in both pine (the tree aged 25) were confirmed. the total amounts of free amino acids i ...19892746471
auxin and ethylene regulation of diameter growth in trees.recent studies on the phytohormonal regulation of seasonal cell-division activity in the cambium, primary-wall radial expansion of cambial derivatives, differentiation of xylem cells, and growth of the cortex in forest trees of the north temperate zone are reviewed. indol-3-ylacetic acid (iaa, auxin) has been characterized by combined gas chromatography-mass spectrometry (gc-ms) in the cambial region of abies balsamea, pinus densiflora, pinus sylvestris and quercus robur. all of the evidence sup ...198814972810
determination of abscisic acid in pinus densiflora by selected ion monitoring.abscisic acid (aba) in the stem of akamatsu (pinus densiflora) was identified and quantified by gas chromatography-mass spectrometry-selected ion monitoring using hexadeuterated aba as an internal standard. tert-butyldimethylsilyl ester was used as a derivative of aba. this derivative had high sensitivity and selectivity for aba determination. aba concentrations in cambial region scrapings were independent of the cessation of cambial activity.198816666340
[studies on the constituents of pinus densiflora sieb. et zucc. leaves. on the ethanol-extractable amino acids]. 19873656089
a demographic analysis of mortality caused by the pine wood nematode (bursaphelenchus xylophilus) and pine sawyer beetles (monochamus alternatus) in pine forests in the seto inland sea-side, japan.demographic characteristics were examined in 51 study sites in naturally occurring pine populations in the seto inland sea-side. the pine forests consisted on average of 51.4% japanese red pine,pinus densiflora, 28.9% japanese black pine,pinus thunbergii, and 19.7% hybrids of the two species, and occurred along an elevatinal gradient from denser, younger secondary forest regrowing after disturbance, to more mature stands at higher elevations. the proportion of dead trees did not vary much among ...198628311773
factors affecting the emission of monoterpenes from red pine (pinus densiflora).the mechanism of monoterpene emission from pinus densiflora was studied using an environmentally controlled gas cabinet. it was found that monoterpene emission rate increases exponentially with temperature and is also influenced by light. these observations were explained reasonably by a mechanism whereby monoterpene emission rate depends on the monoterpene amount in the leaf oil and its saturated vapor pressure.198416663724
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