Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| cell-specific expression of the promoters of two nonlegume hemoglobin genes in a transgenic legume, lotus corniculatus. | the promoters of the hemoglobin genes from the nitrogen-fixing tree parasponia andersonii and the related nonnitrogen-fixing trema tomentosa both confer beta-glucuronidase reporter gene expression to the central zone of the nodules of a transgenic legume, lotus corniculatus. beta-glucuronidase expression was high in the uninfected interstitial cells and parenchyma of the surrounding boundary layer and was low in the rhizobium-infected cells. this contrasts with the expression of both the p. ande ... | 1997 | 9008386 |
| overexpression of a soybean gene encoding cytosolic glutamine synthetase in shoots of transgenic lotus corniculatus l. plants triggers changes in ammonium assimilation and plant development. | a soybean cytosolic glutamine synthetase gene (gs15) was fused with the constitutive 35s cauliflower mosaic virus (camv) promoter in order to direct overexpression in lotus corniculatus l. plants. following transformation with agrobacterium rhizogenes, eight independent lotus transformants were obtained which synthesized additional cytosolic glutamine synthetase (gs) in the shoots. to eliminate any interference caused by the t-dna from the ri plasmid, three primary transformants were crossed wit ... | 1997 | 9151446 |
| expression of an antirrhinum dihydroflavonol reductase gene results in changes in condensed tannin structure and accumulation in root cultures of lotus corniculatus (bird's foot trefoil). | condensed tannins (proanthocyanidins) are an important factor in the nutritive and dietary quality of many forage crops. we report here experiments aimed at altering the levels and monomer composition of condensed tannins (cts) in 'hairy root' cultures of lotus corniculatus (bird's foot trefoil) using genetic manipulation. an antirrhinum majus dihydroflavonol reductase (dfr) cdna was expressed in sense in l. corniculatus and ct levels in transgenic root cultures were analysed. two co-transformed ... | 1997 | 9349268 |
| differential modification of flavonoid and isoflavonoid biosynthesis with an antisense chalcone synthase construct in transgenic lotus corniculatus. | three clonal genotypes of lotus corniculatus l. (bird's foot trefoil) were transformed with an antisense chalcone synthase (chs) gene construct made using a stress induced chs17 cdna from phaseolus vulgaris under the control of the constitutive camv 35s promoter and nos terminator via agrobacterium rhizogenes. after initial screening, ten antisense and five control co-transformation events from each recipient clonal genotype were analysed. after elicitation with glutathione, the level of tannin ... | 1997 | 9349273 |
| four unnamed species of nonsymbiotic rhizobia isolated from the rhizosphere of lotus corniculatus. | previously, we found that genetically diverse rhizobia nodulating lotus corniculatus at a field site devoid of naturalized rhizobia had symbiotic dna regions identical to those of icmp3153, the inoculant strain used at the site (j. t. sullivan, h. n. patrick, w. l. lowther, d. b. scott, and c. w. ronson, proc. natl. acad. sci. usa 92:8985-8989, 1995). in this study, we characterized seven nonsymbiotic rhizobial isolates from the rhizosphere of l. corniculatus. these included two from plants at t ... | 1996 | 8702274 |
| differential expression of the sesbania rostrata leghemoglobin glb3 gene promoter in transgenic legume and non-legume plants. | the involvement of the sesbania rostrata glb3 gene promoter nice (nodule-infected cell expression) element in root-enhanced expression of 5'-srglb3-uida-3'nos chimeric gene was investigated in transgenic nicotiana tabacum plants. the full-length wild-type srglb3 promoter directed root meristem-enhanced expression in transgenic tobacco plants. the expression pattern of nine selected srglb3 promoter mutations in the nice element was examined in transgenic tobacco plants and compared with the patte ... | 1996 | 8806425 |
| novel and complex chromosomal arrangement of rhizobium loti nodulation genes. | a mutational and structural analysis of rhizobium loti nodulation genes in strains nzp2037 and nzp2213 was carried out. unlike the case with other rhizobium strains examined to date, nodb was found on an operon separate from nodacij. sequence analysis of the nodacij and nodb operon regions confirm that r. loti common nod genes have a gene organization different from that of other rhizobium spp. at least 4 copies of nodd-like sequences were identified in r. loti. the complete nucleotide sequence ... | 1996 | 8850088 |
| a system for tissue-specific copper-controllable gene expression in transgenic plants: nodule-specific antisense of aspartate aminotransferase-p2. | a vector system, based on copper controllable gene expression, has been developed to give control over place as well as time of expression of an introduced gene. this system consists of two elements: (1) the yeast ace1 gene encoding a metallo-regulatory transcription factor, ace1, under control of either an organ-specific or a constitutive promoter; and (2) a gene of interest under control of a chimaeric promoter consisting of the 46 bp tata fragment of the camv 35s rna promoter linked to four r ... | 1996 | 8866892 |
| controlling internal parasites in grazing ruminants without recourse to anthelmintics: approaches, experiences and prospects. | interest in ways of raising stock without using anthelmintics has been stimulated by the desire for intensive grazing systems to adopt more sustainable methods of internal parasite control and by potential premiums for "organic" produce. this requires grazing management systems which are practicable and achieve levels of parasite control sufficient to meet realistic production objectives. this paper will summarise 3 years experience of lamb and cattle production without a recourse to anthelminti ... | 1996 | 8923145 |
| nodulating strains of rhizobium loti arise through chromosomal symbiotic gene transfer in the environment. | rhizobia were isolated from nodules off a stand of lotus corniculatus established with a single inoculant strain, icmp3153, 7 years earlier in an area devoid of naturalized rhizobium loti. the isolates showed diversity in growth rate, spe i fingerprint of genomic dna, and hybridization pattern to genomic dna probes. the 19% of isolates that grew at the same rate as strain icmp3153 were the only isolates that had the same fingerprint as strain icmp3153. sequencing of part of the 16s rrna gene of ... | 1995 | 7568057 |
| pasture management strategies for reducing the risk of legume bloat in cattle. | results from two decades (1973-1993) of bloat research at kamloops are reviewed. the trials were conducted with groups of ruminally fistulated cattle either grazing or fed daily fresh-cut alfalfa (medicago sativa) herbage. studies were conducted during the growing season (may to september) and in the fall (october and november). the alfalfa was usually in the vegetative to early bloom stages of growth. visual assessments of bloat severity on a scale of 1 to 5 were made .5 to 2 h after feeding st ... | 1995 | 7665381 |
| symbiotic and nonsymbiotic hemoglobin genes of casuarina glauca. | casuarina glauca has a gene encoding hemoglobin (cashb-nonsym). this gene is expressed in a number of plant tissues. casuarina also has a second family of hemoglobin genes (cashb-sym) expressed at a high level in the nodules that casuarina forms in a nitrogen-fixing symbiosis with the actinomycete frankia. both the nonsymbiotic and symbiotic genes retained their specific patterns of expression when introduced into the legume lotus corniculatus. we interpret this finding to mean that the controls ... | 1995 | 7756831 |
| identification of several soybean cytosolic glutamine synthetase transcripts highly or specifically expressed in nodules: expression studies using one of the corresponding genes in transgenic lotus corniculatus. | a dna fragment containing sequences hybridizing to the 5' region of gs15, a gene encoding soybean cytosolic glutamine synthetase, was isolated from a soybean genomic library. mapping and partial sequence analysis of the genomic clone revealed that it encodes a cytosolic gs gene, gs21, which is different from gs15. in parallel, a number of cdna clones encoding cytosolic gs were isolated using the coding region of pgs20 as a probe (pgs20 is a cdna clone which corresponds to a transcript of the gs1 ... | 1995 | 7865779 |
| agrobacterium rhizogenes as a vector for transforming higher plants. application in lotus corniculatus transformation. | 1995 | 8563829 | |
| suspected photosensitisation in lambs grazing birdsfoot trefoil (lotus corniculatus). | suspected photosensitisation occurred in three groups of lambs grazing birdsfoot trefoil (lotus corniculatus c.v. grasslands goldie). in one group, sucking lambs aged about 10 weeks, grazing birdsfoot trefoil, developed skin lesions while lambs of a similar age and from the same flock grazing lucerne (medicago sativa) or a mixed sward of both species showed no signs of photosensitisation. affected lambs had lesions on their backs and ears. in a few animals the tips of the ears were shortened by ... | 1995 | 16031826 |
| oxygen-induced membrane depolarizations in legume root nodules (possible evidence for an osmoelectrical mechanism controlling nodule gas permeability). | various stresses trigger rapid and reversible decreases in the o2 permeability (po) of legume root nodules. several possible mechanisms have been proposed, but no supporting data have previously been presented that meet the requirements for both rapidity and reversibility. stomatal regulation of gas permeability in leaves involves electrically driven fluxes of inorganic osmoticants, so we investigated the possibility of a somewhat similar mechanism in nodules. we used microelectrodes to monitor ... | 1995 | 12228468 |
| nitrate effects on nodule oxygen permeability and leghemoglobin (nodule oximetry and computer modeling). | two current hypotheses to explain nitrate inhibition of nodule function both involve decreased o2 supply for respiration in support of n2 fixation. this decrease could result from either (a) decreased o2 permeability (po) of the nodule cortex, or (b) conversion of leghemoglobin (lb) to an inactive, nitrosyl form. these hypotheses were tested using alfalfa (medicago sativa l. cv weevlchek) and birdsfoot trefoil (lotus corniculatus l. cv fergus) plants grown in growth pouches under controlled cond ... | 1995 | 12228439 |
| genetic modification of condensed tannin biosynthesis in lotus corniculatus. 1. heterologous antisense dihydroflavonol reductase down-regulates tannin accumulation in "hairy root" cultures. | an antisense dihydroflavonol reductase (dfr) gene-construct made using the cdna for dfr from antirrhinum majus was introduced into the genome of a series of clonal genotypes of lotus corniculatus via agrobacterium rhizogenes. after initial screening, 17 antisense and 11 control transformation events were analysed and tannin levels found to be reduced in antisense root cultures. the effect of this antisense construct, (pmaj2), which consisted of the 5' half of the dfr cdna sequence, was compared ... | 1994 | 24190536 |
| proanthocyanidins inhibit hydrolysis of leaf proteins by rumen microflora in vitro. | proanthocyanidins (condensed tannins; pa) purified from the leaves of forage legumes trifolium arvense, lotus pedunculatus, lotus corniculatus, dorycnium rectum, coronilla varia, onobrychis viciifolia, or hedysarum coronarium, were added to soluble lucerne (medicago sativa) leaf protein and incubated with strained rumen fluid in vitro. fractions were collected and frozen immediately. denatured proteins were fractionated by sodium dodecylsulphate-polyacrylamide gel electrophoresis (sds-page), sta ... | 1994 | 8031741 |
| a protein binding at-rich sequence in the soybean leghemoglobin c3 promoter is a general cis element that requires proximal dna elements to stimulate transcription. | a nodule nuclear factor, nat2, interacts with two at-rich binding sites (nat2 bs1 and nat2 bs2) in the soybean leghemoglobin (lb) c3 promoter. in transgenic lotus corniculatus nodules, an oligonucleotide containing nat2 bs1 activated an inactive -159 lbc3 promoter when placed immediately upstream of the promoter. the activation was independent of the orientation of nat2 bs1 but was dependent on its position in the promoter. the abilities of different mutated binding sites to activate expression ... | 1994 | 8038605 |
| effect of condensed tannins from birdsfoot trefoil on endoglucanase activity and the digestion of cellulose filter paper by ruminal fungi. | the ruminal fungi neocallimastix frontalis re1, neocallimastix patriciarum 27, piromyces communis 22, and orpinomyces joyonii 19-2 were examined for their ability to digest filter paper in the presence of condensed tannins from birdsfoot trefoil (lotus corniculatus l.). for all four fungi, inhibition of endoglucanases was evident at 100 micrograms condensed tannins.ml-1 with nearly complete inhibition at 300 micrograms condensed tannins.ml-1. at 100 and 200 micrograms condensed tannins.ml-1, the ... | 1994 | 8039053 |
| site-specific mutagenesis of the nodule-infected cell expression (nice) element and the at-rich element atre-bs2* of the sesbania rostrata leghemoglobin glb3 promoter. | sesbania rostrata leghemoglobin glb3 (srglb3) promoter sequences responsible for expression in infected cells of transgenic lotus corniculatus nodules were delimited to a 78-bp dral-hinfl fragment. this region, which is located between coordinates -194 to -116 relative to the start codon of the srglb3 gene, was named the nodule-infected cell expression (nice) element. insertion of the nice element into the truncated nopaline synthase promoter was found to confer a nodule-specific expression patt ... | 1994 | 8180496 |
| the effect of condensed tannins in lotus corniculatus on plasma metabolism of methionine, cystine and inorganic sulphate by sheep. | fresh lotus corniculatus containing 27 g extractable condensed tannin (ct)/kg dry matter (dm) and 8 g bound ct/kg dm was fed at hourly intervals to sheep held in metabolism cages to study the effects of ct on nutrient digestion and on metabolism of methionine, cystine and inorganic sulphate in plasma. polyethylene glycol (peg) was continuously infused into the rumen of half the sheep to remove the effects of ct. principal measurements in the two groups were plasma irreversible loss (irl) rate an ... | 1994 | 7827012 |
| the expression of a chimeric phaseolus vulgaris nodulin 30-gus gene is restricted to the rhizobially infected cells in transgenic lotus corniculatus nodules. | in phaseolus vulgaris there is a nodulin family, npv30, of ca. 30 kda, as detected in an in vitro translation assay [2]. we isolated a gene (npv30-1) for one of the members of this family. the nucleotide sequence of the promoter of npv30-1 contains nodule-specific motifs common to other late nodulin genes. the promoter was fused to the gus reporter gene; this chimeric fusion was introduced into lotus corniculatus via agrobacterium rhizogenes transformation. gus activity was only detected in the ... | 1994 | 7858234 |
| soybean nodulin-26 gene encoding a channel protein is expressed only in the infected cells of nodules and is regulated differently in roots of homologous and heterologous plants. | nodulin-26 (n-26) is a major peribacteroid membrane protein in soybean root nodules. the gene encoding this protein is a member of an ancient gene family conserved from bacteria to humans. n-26 is specifically expressed in root nodules, while its homolog, soybean putative channel protein, is expressed in vegetative parts of the plant, with its highest level in the root elongation zone. analysis of the soybean n-26 gene showed that its four introns mark the boundaries between transmembrane domain ... | 1993 | 7689881 |
| the quest for a herbal contraceptive. | an oral herbal contraceptive would allow couples control their fertility without consulting a health worker, which in turn would likely markedly increase the number of couples practicing family planning. other advantages of such a contraceptive would include the familiarity rural people have with herbal medicines, the fewer side effects associated with herbal preparations, their ready availability from local sources, and protection of privacy. there are many references to plants in india with ... | 1993 | 8241931 |
| interaction of a rhizobial dna-binding protein with the promoter region of a plant leghemoglobin gene. | a nucleotide sequence was identified approximately 650 bp upstream of the sesbania rostrata leghemoglobin gene srglb3 start codon, which interacts specifically with a proteinaceous dna-binding factor found in nodule extracts but not in extracts from leaves or roots. the binding site for this factor was delimited using footprinting techniques. the dna-binding activity of this factor was found to be heat stable, dependent on divalent cations, and derived from the (infecting) azorhizobium caulinoda ... | 1993 | 8278541 |
| effects of condensed tannins on endoglucanase activity and filter paper digestion by fibrobacter succinogenes s85. | the effect of condensed tannins from birdsfoot trefoil (lotus corniculatus l.) on the cellulolytic rumen bacterium fibrobacter succinogenes s85 was examined. condensed tannins inhibited endoglucanase activity in the extracellular culture fluid, at concentrations as low as 25 mug ml. in contrast, cell-associated endoglucanase activity increased in concentrations of condensed tannins between 100 and 300 mug ml. inhibition of endoglucanase activity in both the extracellular and the cell-associated ... | 1993 | 16348990 |
| minimal enhancer elements of the leghemoglobin lba and lbc3 gene promoters from glycine max l. have different properties. | the characteristics of the soybean leghemoglobin lba gene promoter were analyzed and important promoter elements from the lba and lbc3 promoters were compared using transgenic lotus corniculatus plants. a 5' deletion analysis of the lba promoter delimited two cis-acting elements controlling expression: a distal positive element (-1254, -884) required for expression and a proximal element (-285, -60) essential for full-level activity. in contrast to the corresponding region of the lbc3 promoter, ... | 1993 | 8400139 |
| gene flow inferred from seed dispersal and pollinator behaviour compared to dna analysis of restriction site variation in a patchy population of lotus corniculatus l. | gene flow was investigated in a natural population of lotus corniculatus l. (fabaceae) using a combination of pollen and seed dispersal studies and a recombinant dna technique. the population is spatially heterogeneous and grows with empetrum nigrum. l. corniculatus is pollinated by the pollen-collecting bumblebee bombus lapidarius l. most pollinator flights occurred within patches, as bees usually visit nearest-neighbour plants, show no marked directionality, and forage mostly within patches. g ... | 1992 | 28312883 |
| functional analysis of the promoter region of a nodule-enhanced glutamine synthetase gene from phaseolus vulgaris l. | the 5'-flanking region of gln-gamma, the nodule-enhanced glutamine synthetase gene from phaseolus vulgaris l., has been analysed for cis-regulatory elements using a series of 5' deletions and hybrid gln-gamma:: camv 35s promoters. the promoters were fused to the uida reporter gene and their activities tested in two heterologous expression systems. in the first system, the chimaeric genes were transferred to lotus corniculatus l. using agrobacterium rhizogenes and their expression was studied in ... | 1992 | 1353692 |
| forcing expression of a soybean root glutamine synthetase gene in tobacco leaves induces a native gene encoding cytosolic enzyme. | glutamine synthetase (gs; ec 6.3.1.2) is present in different subcellular compartments in plants. it is located in the cytoplasm in root and root nodules while generally present in the chloroplasts in leaves. the expression of gs gene(s) is enhanced in root nodules and in soybean roots treated with ammonia. we have isolated four genes encoding subunits of cytosolic gs from soybean (glycine max l. cv. prize). promoter analysis of one of these genes (gs15) showed that it is expressed in a root-spe ... | 1992 | 1356501 |
| ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic lotus corniculatus. | a full-length cdna clone encoding cytosolic glutamine synthetase (gs), expressed in roots and root nodules of soybean, was isolated by direct complementation of an escherichia coli gln a- mutant. this sequence is induced in roots by the availability of ammonia. a 3.5-kilobase promoter fragment of a genomic clone (lambda gs15) corresponding to this cdna was isolated and fused with a reporter [beta-glucuronidase (gus)] gene. the gs-gus fusion was introduced into a legume (lotus corniculatus) and a ... | 1991 | 1688099 |
| phenylpropanoid defence responses in transgenic lotus corniculatus 1. glutathione elicitation of isoflavan phytoalexins in transformed root cultures. | when agrobacterium rhizogenes transformed root cultures of lotus corniculatus were treated with glutathione, isoflavan phytoalexins accumulated in both tissue and culture medium. this accumulation of phytoalexins was preceded by a transient increase in the activity of phenylalanine ammonia lyase (pal). elicitation of pal occurred throughout the growth curve of lotus 'hairy roots' and in different sectors of transformed root material. | 1991 | 24221394 |
| a two-component nodule-specific enhancer in the soybean n23 gene promoter. | the two positive cis elements in the soybean nodulin n23 gene promoter were investigated in transgenic lotus corniculatus plants and shown to constitute a two-component nodule-specific enhancer. equal quantitative contributions from the two components were suggested by the similar expression level of chimeric n23-chloramphenicol acetyltransferase genes after deletion of either the distal positive element (pe-a, -320 to -298) or the proximal positive element (pe-b, -257 to -165). a combined effec ... | 1991 | 1820821 |
| additional hosts for the ring nematode, criconemella xenoplax. | some common legumes and weeds indigenous to peach orchards in south carolina were tested in greenhouse experiments to determine their host suitability for criconemella xenoplax. legumes that were hosts for the nematode were dwarf english trefoil (lotus corniculatus var. arvensis), big trefoil (l. uliginosis), birdsfoot trefoil (l. corniculatus), narrowleaf birdsfoot trefoil (l. tenuis), ball clover (trifolium nigrescens), rose clover (t. hirtum), subterranean clover (t. subterraneum), striate le ... | 1990 | 19287692 |
| variation in a single protoplast- and seed-derived population of lotus corniculatus l. | lotus comiculatus l. is a widely cultivated, outbreeding, leguminous forage crop. seventy-one plants, most of which were tetraploid, were regenerated from calli derived from a single protoplast. their morphological and agronomic traits were evaluated and compared with those of the seed-produced population. the variances of most of the traits in the protoplast-derived (protoclonal) population were smaller than those of the seed-produced population. mean values of all the traits of the protoclonal ... | 1990 | 24221102 |
| functional analysis of the sesbania rostrata leghemoglobin glb3 gene 5'-upstream region in transgenic lotus corniculatus and nicotiana tabacum plants. | expression of the sesbania rostrata leghemoglobin glb3 gene was analyzed in transgenic lotus corniculatus and tobacco plants harboring chimeric glb3-uida (gus) gene fusions to identify cis-acting elements involved in nodule-specific gene expression and general transcriptional control. a 1.9-kilobase fragment of the glb3 5'-upstream region was found to direct a high level of nodule-specific beta-glucuronidase (gus) activity in l. corniculatus, restricted to the rhizobium-infected cells of the nod ... | 1990 | 2136628 |
| nonlegume hemoglobin genes retain organ-specific expression in heterologous transgenic plants. | hemoglobin genes from the nitrogen-fixing nonlegume parasponia andersonii and the related non-nitrogen-fixing nonlegume trema tomentosa have been isolated [landsmann et al. (1986). nature 324, 166-168; bogusz et al. (1988). nature 331, 178-180]. the promoters of these genes have been linked to a beta-glucuronidase reporter gene and introduced into both the nonlegume nicotiana tabacum and the legume lotus corniculatus. both promoters directed root-specific expression in transgenic tobacco. when t ... | 1990 | 2136637 |
| interdependence and nodule specificity of cis-acting regulatory elements in the soybean leghemoglobin lbc3 and n23 gene promoters. | the qualitative and quantitative contributions of four separate cis-acting dna elements controlling the root nodule-specific soybean leghemoglobin lbc3 gene were analyzed in transgenic lotus corniculatus plants. expression from internal deletions in the 5' region between positions -49 and -1956 was monitored from a cat reporter gene. the strong positive element (spe; -1090, -947) responsible for high-level expression was demonstrated to be an organ-specific element by deleting proximal nodule-sp ... | 1990 | 2338938 |
| high sensitivity to auxin is a common feature of hairy root. | the responses to auxin of lycopersicon esculentum roots transformed by (t(l)+t(r))-dna of the ri plasmid of agropine-type agrobacterium rhizogenes strain 15834 and catharanthus trichophyllus roots transformed by the (t(l)+t(r))-dna, and by t(l)- or t(r)- dna alone of the same bacterial strain were compared to that of their normal counterparts. the transmembrane electrical potential difference of root protoplasts was measured as a function of the concentration of exogenous naphthalene acetic acid ... | 1990 | 16667748 |
| hairy roots are more sensitive to auxin than normal roots. | responses to auxin of lotus corniculatus root tips or protoplasts transformed by agrobacterium rhizogenes strains 15834 and 8196 were compared to those of their normal counterparts. three different types of experiments were performed, involving long-term, medium-term, or short-term responses to a synthetic auxin, 1-naphthaleneacetic acid. root tip elongation, proton excretion by root tips, and transmembrane electrical potential difference of root protoplasts were measured as a function of exogen ... | 1988 | 16593928 |
| root nodule specific gene regulation: analysis of the soybean nodulin n23 gene promoter in heterologous symbiotic systems. | the nodulin n23 gene promoter was analysed in transgenic plants using the chloramphenicol acetyltransferase (cat) coding sequence as a reporter. a 5' flanking region of less than 1 kb was sufficient for the organ-specific expression of a chimeric n23-cat-3'lbc3 gene in root nodules formed on lotus corniculatus and trifolium repens after infection by their respective rhizobium symbionts. expression was regulated at the level of rna in both species of transgenic plants. promoter deletion analysis ... | 1988 | 3340542 |
| the effect of condensed tannins on the site of digestion of amino acids and other nutrients in sheep fed on lotus corniculatus l. | sheep were used to evaluate the nutritional consequences of a low condensed-tannin concentration (22 g/kg dry matter (dm)) in lotus (lotus corniculatus l.) (control group) compared with lotus given to sheep receiving intraruminal polyethylene glycol (peg) infusion (peg group). peg selectively binds to tannins and prevents tannins from binding proteins. dm intakes (1430 (se 28) g/d) and digestibility of energy (663 (se 4.5) kj/mj intake) were similar for both groups but the apparent digestion of ... | 1987 | 3801377 |
| 5' analysis of the soybean leghaemoglobin lbc(3) gene: regulatory elements required for promoter activity and organ specificity. | the soybean leghaemoglobin lbc(3) gene promoter was analysed in transgenic lotus corniculatus plants. hybrid-promoter constructions and 5' deletions were studied using chimeric genes composed of the various promoters, the chloramphenicol acetyltransferase (cat) coding sequence and the lbc(3) 3' flanking region. a 5' bal31 deletion series mapped a strong positive regulatory element between -1100 and -950. a weaker element located between -230 and -170 defined the minimum 5' region required for de ... | 1987 | 16453808 |
| expression of a complete soybean leghemoglobin gene in root nodules of transgenic lotus corniculatus. | the complete soybean leghemoglobin lbc(3) gene was transferred into the legume lotus corniculatus using an agrobacterium rhizogenes vector system. organ-specific expression of the soybean gene was observed in root nodules formed on regenerated transgenic plants after infection with rhizobium loti. the primary transcript was processed in the same way as in soybean nodules and the resulting mrna was translated into lbc(3) protein. quantitative determination of the lbc(3) protein in nodules of tran ... | 1987 | 16593870 |
| why do honeybees reject certain flowers? | honeybees often approach flowers of lotus corniculatus and then fly away without attempting to extract nectar. these rejected flowers contained 41% less nectar than my random sample. the accepted flowers contained 24% more nectar than my random sample. the differences among these three flower-groups were due to differences in the percent of empty flowers in each group rather than the differences in the absolute amount of nectar. honeybees increased their foraging efficiency by accepting less emp ... | 1986 | 28311617 |
| transformation of forage legumes using agrobacterium tumefaciens. | galls were induced in six species of forage legumes following inoculation with wild-type strains of a. tumefaciens. the plant species was more influential than the bacterial strain in determining the type of tumour produced. inoculation of medicago sativa resulted in small, disorganised tumours. the three trifolium species, t. repens, t. hybridum and t. pratense, formed galls which tended to produce roots and both onobrychis viciifolia and lotus corniculatus produced teratomatous galls. the shoo ... | 1986 | 24247771 |
| in vitro digestion of bloat-safe and bloat-causing legumes by rumen microorganisms: gas and foam production. | leaves of three bloat-safe legumes -- birdsfoot trefoil (lotus corniculatus l.), sainfoin (onobrychis viciaefolia scop.), and cicer milkvetch (astralagus cicer l.) -- and of three bloat-causing legumes -- alfalfa (medicago sativa l.), red clover (trifolium pratense l.), and white clover (trifolium repens l.) -- were incubated with strained rumen fluid or with mixed rumen fluid and solids. gas released was measured during the early period (0 to 22 h) of this in vitro digestion. gas volume was gre ... | 1980 | 7419778 |
| chemotaxis of rhizobium spp. to a glycoprotein produced by birdsfoot trefoil roots. | rhizobium spp. show chemotaxis to plant root exudates. a glycoprotein has been isolated from the root exudates of birdsfoot trefoil, lotus corniculatus, which, at micromolar concentrations, attracts six strains of rhizobia. this glycoprotein has been given the trivial name trefoil chemotactin and contains approximately twice as much protein as carbohydrate. gel filtration of trefoil chemotactin on a bio-gel a-1.5m column gave a molecular weight of approximately 60,000. trefoil chemotactin repres ... | 1977 | 17776952 |
| efficacy of multiple applications of oxamyl and phenamiphos for control of pratylenchus penetrans in birdsfoot trefoil. | preplant soil applications of granular phenamiphos effectively reduced pratytenchus penetrans in soil during the seeding year and 1 year after, and in the roots of birdsfoot trefoil 2 years after seeding. forage yields were increased in the season following application of phenamiphos, but stands of plants/m(2) were not greater (p = 0.05) than those in the checks 1 and 2 years after treatment. additional spring applications of phenamiphos 1 and 2 years after seeding further reduced numbers of nem ... | 1976 | 19308245 |