Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| A case of Stenotrophomonas maltophilia keratitis effectively treated with moxifloxacin. | A 70-year-old man with a long history of diabetes mellitus presented to our hospital (Department of Ophthalmology, Sahm Yook Medical Center, Seoul, Korea) complaining of severe ocular pain and visual disturbance in his left eye that had started three days prior to admission. A round 3.7 × 5.0 mm dense central stromal infiltrate with an overlying epithelial defect was noted on slit-lamp examination. Following corneal scrapings and culture, topical 0.5% moxifloxacin and 0.5% tobramycin were admini ... | 2011 | 21976944 |
| stenotrophomonas maltophilia in the respiratory tract of medical intensive care unit patients. | the purpose of this study was to investigate characteristics of critically ill patients with stenotrophomonas maltophilia (s. maltophilia) isolated from the respiratory tract, to identify risk factors for s. maltophilia-pneumonia and intensive care unit (icu) mortality and to analyze antibiotic susceptibility of s. maltophilia. this was a retrospective analysis of 64 medical icu patients with s. maltophilia in the respiratory tract. thirty-six patients fulfilled the criteria for diagnosis of pne ... | 2011 | 22057419 |
| the antimicrobial efficacy of silver on antibiotic-resistant bacteria isolated from burn wounds. | the antibiotic-resistant bacteria are a major concern to wound care because of their ability to resist many of the antibiotics used today to treat infections. consequently, other antimicrobials, in particular ionic silver, are considered ideal topical agents for effectively helping to manage and prevent local infections. little is known about the antimicrobial efficacy of ionic silver on antibiotic-resistant bacteria at different ph values. consequently, in this study our aim was to evaluate the ... | 2011 | 22182219 |
| screening for novel quorum-sensing inhibitors to interfere with the formation of pseudomonas aeruginosa biofilm. | the objective of this study was to screen for novel quorum-sensing inhibitors (qsis) from traditional chinese medicines (tcms) that inhibit bacterial biofilm formation. six of 46 active components found in tcms were identified as putative qsis based on molecular docking studies. of these, three compounds inhibited biofilm formation by pseudomonas aeruginosa and stenotrophomonas maltophilia at a concentration of 200 µm. a fourth compound (emodin) significantly inhibited biofilm formation at 20 µm ... | 2011 | 21852522 |
| calcite biomineralization by bacterial isolates from the recently discovered pristine karstic herrenberg cave. | karstic caves represent one of the most important subterranean carbon storages on earth and provide windows into the subsurface. the recent discovery of the herrenberg cave, germany, gave us the opportunity to investigate the diversity and potential role of bacteria in carbonate mineral formation. calcite was the only mineral observed by raman spectroscopy to precipitate as stalactites from seepage water. bacterial cells were found on the surface and interior of stalactites by confocal laser sca ... | 2011 | 22179248 |
| effects of clinical breakpoint changes in clsi guidelines 2010/2011 and eucast guidelines 2011 on antibiotic susceptibility test reporting of gram-negative bacilli. | objectives: the aim of this study was to analyse the effects of clinical breakpoint changes in clsi 2010 and 2011 guidelines and eucast 2011 guidelines on antibiotic susceptibility testing (ast) reports. methods: in total, 3713 non-duplicate clinical isolates of enterobacteriaceae, pseudomonas aeruginosa, stenotrophomonas maltophilia and acinetobacter baumannii were analysed. inhibition zone diameters were determined for β-lactams, carbapenems, fluoroquinolones, aminoglycosides and trimethoprim/ ... | 2011 | 22167240 |
| Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microrganisms in deep sea reservoirs? | ABSTRACT: Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence from in vitro experiments with samples of petroleum formation water and oils from Pampo Field indicate that petroleum biodegradation is more likely to be a joint achievement of both aerobic and anaerobic bacterial consortia, refining our previous observations of aerobic degradation. ... | 2011 | 22196374 |
| prognosis of acute respiratory distress syndrome (ards) in neutropenic cancer patients. | up today, no study has been specifically designed to identify determinants of death neutropenic cancer patients presenting ards. the aim of this study was to identify early predictive factors of 28-day mortality in these patients. factors associated with 28-day mortality during icu stay were also described.seventy consecutive cancer patients with ards and neutropenia were prospectively analysed over a 6-year period.mortality at 28-day was 63%. factors independently associated with good prognosis ... | 2011 | 22135281 |
| health care-associated pneumonia (hcap): empiric antibiotics targeting methicillin-resistant staphylococcus aureus (mrsa) and pseudomonas aeruginosa predict optimal outcome. | inappropriate initial antimicrobial therapy (iiat) has been associated with decreased survival in patients with health care-associated pneumonia (hcap). we performed a study to determine whether empiric hcap antibiotic regimens targeting methicillin-resistant staphylococcus aureus (mrsa) and pseudomonas aeruginosa are associated with greater appropriate therapy. a retrospective cohort study of culture-positive hcap over 6 years (january 2003-december 2008) was performed at barnes-jewish hospital ... | 2011 | 22033455 |
| antibacterial effects of theaflavin and synergy with epicatechin against clinical isolates of acinetobacter baumannii and stenotrophomonas maltophilia. | stenotrophomonas maltophilia and acinetobacter baumannii are recognised as important nosocomial pathogens; however, due to their intrinsic resistance to multiple antibiotics, treatment options are limited. polyphenols from black tea have been shown to possess antibacterial action. in this study, the antibacterial effects of various concentrations of theaflavin as well as combinations of theaflavin and epicatechin were determined using the disk diffusion assay. the results showed strong antibacte ... | 2011 | 21885260 |
| cloning and overexpression of ketopantoic acid reductase gene from stenotrophomonas maltophilia and its application to stereospecific production of d: -pantoic acid. | ketopantoic acid (kpa) reductase catalyzes the stereospecific reduction of ketopantoic acid to d: -pantoic acid. based on the n-terminal amino acid sequence of kpa reductase from stenotrophomonas maltophilia 845, the kpa reductase gene was cloned from s. maltophilia nbrc14161 and sequenced. this gene contains an open reading frame of 777 bp encoding 258 amino acid residues, and the deduced amino acid sequence showed high similarity to the sdr superfamily proteins. an expression vector, petsmkpr, ... | 2011 | 22083276 |
| Effective cohorting and "superisolation" in a single intensive care unit in response to an outbreak of diverse multi-drug-resistant organisms. | Cohorting patients in dedicated hospital wards or wings during infection outbreaks reduces transmission of organisms, yet frequently, this may not be feasible because of inadequate capacity, especially in the intensive care unit (ICU). We hypothesized that cohorting isolation patients in one geographic location in a single ICU and using enhanced isolation procedures ("superisolation") can prevent the further spread of highly multi-drug-resistant organisms (MDRO). | 2011 | 21936667 |
| Modulation of FAD-dependent monooxygenase activity from aromatic compounds-degrading Stenotrophomonas maltophilia strain KB2. | The purpose of this study was purification and characterization of phenol monooxygenase from Stenotrophomonas maltophilia strain KB2, enzyme that catabolises phenol and its derivatives through the initial hydroxylation to catechols. The enzyme requires NADH and FAD as a cofactors for activity, catalyses hydroxylation of a wide range of monocyclic phenols, aromatic acids and dihydroxylated derivatives of benzene except for catechol. High activity of this monooxygenase was observed in cell extrac ... | 2011 | 21927719 |
| chronic stenotrophomonas maltophilia infection and exacerbation outcomes in cystic fibrosis. | chronic stenotrophomonas maltophilia infection is a risk factor for pulmonary exacerbation in cystic fibrosis (cf) but its impact on subsequent clinical outcomes is unknown. the aim of this study was to determine the effect of chronic s. maltophilia infection and associated antimicrobial therapy on the recovery of forced expiratory lung volume in 1s (fev(1)) following pulmonary exacerbation. | 2011 | 21849265 |
| Enhancement of antibiotic susceptibility of Stenotrophomonas maltophilia using a polyclonal antibody developed against an ABC multidrug efflux pump. | Stenotrophomonas maltophilia is an emerging nosocomial pathogen capable of causing healthcare-associated infections, including pneumonia and bacteremia. Intrinsic resistance in S. maltophilia is exhibited towards many broad-spectrum antibiotics, and treatment recommendations are controversial. One of the major causes of antimicrobial resistance is attributed to a robust array of efflux pumps that extrude drug compounds from the cell. Using checkerboard and growth kinetic assays, we evaluated the ... | 2011 | 21942332 |
| exploring characteristics of bioelectricity generation and dye decolorization of mixed and pure bacterial cultures from wine-bearing wastewater treatment. | this study uncovered microbial characteristics of bioelectricity generation and dye decolorization in single-chamber microbial fuel cells (mfcs) using activated sludge for wine-containing wastewater treatment. phylogenetic tree analysis on 16s rrna gene fragments indicated that the predominant strains on anodic biofilm in acclimatized mfcs were gamma-proteobacteria aeromonas punctata niu-p9, pseudomonas plecoglossicida niu-y3, pseudomonas koreensis niu-x8, acinetobacter junii niu-y8, stenotropho ... | 2011 | 20859654 |
| the third-generation p-glycoprotein inhibitor tariquidar may overcome bacterial multidrug resistance by increasing intracellular drug concentration. | the use of efflux pump inhibitors may be a powerful strategy to overcome transporter-mediated bacterial multidrug resistance. in the present study, we set out to investigate the potency of tariquidar, a third-generation p-glycoprotein inhibitor in clinical development, for overcoming bacterial resistance towards ciprofloxacin. | 2011 | 21393173 |
| smqnrr, a deor-type transcriptional regulator, negatively regulates the expression of smqnr and smtcra in stenotrophomonas maltophilia. | to characterize the role of smqnrr in the expression of smqnr and smtcra, and the role of smtcra in drug resistance in stenotrophomonas maltophilia. | 2011 | 21393212 |
| in vitro activity of ceftaroline against gram-positive and gram-negative pathogens isolated from patients in canadian hospitals in 2009. | the in vitro activities of ceftaroline and comparative agents were determined for a collection of the most frequently isolated bacterial pathogens from hospital-associated patients across canada in 2009 as part of the ongoing canward surveillance study. in total, 4,546 isolates from 15 sentinel canadian hospital laboratories were tested using the clinical and laboratory standards institute (clsi) broth microdilution method. compared with other cephalosporins, including ceftobiprole, cefepime, an ... | 2011 | 21402844 |
| pme-1, an extended-spectrum ß-lactamase identified in pseudomonas aeruginosa. | a novel extended-spectrum ß-lactamase (esbl) was identified in a pseudomonas aeruginosa clinical isolate obtained from a patient admitted to a hospital in pennsylvania in 2008. the patient had a prolonged hospitalization in a hospital in dubai, united arab emirates, before being transferred to the united states. the novel esbl, designated pme-1 (pseudomonas aeruginosa esbl 1), is a molecular class a, bush-jacoby-medeiros group 2be enzyme and shared 50, 43, and 41% amino acid identity with the l2 ... | 2011 | 21402845 |
| [biodiversity of mesophilic microbial community bynd-8 capability of lignocellulose degradation and its effect on biogas production]. | the biodiversity of a mesophilic microbial community bynd-8 capable of degrading lignocellulose at 30 degrees c was detected using denaturing gradient gel electrophoresis (dgge) and the isolation of pure cultures, and the effect of the liquid of rice straw degradation by bynd-8 on biogas production was measured. six bacterial strains were isolated using peptone cellulose solution medium, and the highest similarities of their 16s rdna gene sequences to serratia sp. psgb 13, s. marcescens strain u ... | 2011 | 21404695 |
| adhesion of stenotrophomonas maltophilia to mouse tracheal mucus is mediated through flagella. | adhesion of stenotrophomonas maltophilia, an opportunistic pathogen, to different surfaces has been reported in the literature. however, its ability to adhere to mucus and the involvement of different bacterial appendages in this process has not been elucidated. in this study, bacterial adhesion to mouse tracheal mucus as well as the role of flagella in the adhesion process were investigated using clinical isolates of s. maltophilia. all the clinical isolates adhered to mouse tracheal mucus to v ... | 2011 | 21415208 |
| separation of the bacterial species, escherichia coli, from mixed-species microbial communities for transcriptome analysis. | the study of bacterial species interactions in a mixed-species community can be facilitated by transcriptome analysis of one species in the community using cdna microarray technology. however, current applications of microarrays are mostly limited to single species studies. the purpose of this study is to develop a method to separate one species, escherichia coli as an example, from mixed-species communities for transcriptome analysis. | 2011 | 21418656 |
| application of a continuously stirred tank bioreactor (cstr) for bioremediation of hydrocarbon-rich industrial wastewater effluents. | a continuously stirred tank bioreactor (cstr) was used to optimize feasible and reliable bioprocess system in order to treat hydrocarbon-rich industrial wastewaters. a successful bioremediation was developed by an efficient acclimatized microbial consortium. after an experimental period of 225 days, the process was shown to be highly efficient in decontaminating the wastewater. the performance of the bioaugmented reactor was demonstrated by the reduction of cod rates up to 95%. the residual tota ... | 2011 | 21419572 |
| acute obstructive suppurative pancreatic ductitis. | 2011 | 21421076 | |
| correlation between antibiotic consumption and resistance of gram-negative bacteria causing healthcare-associated infections at a university hospital in taiwan from 2000 to 2009. | this study investigated the correlation between antibiotic consumption and antimicrobial resistance in gram-negative bacteria causing healthcare-associated infections at a university hospital in taiwan from 2000 to 2009. | 2011 | 21436153 |
| monitoring the bacterial community of maize silage stored in a bunker silo inoculated with enterococcus faecium, lactobacillus plantarum and lactobacillus buchneri. | to monitor variations in the bacterial community and fermentation products of maize silage within and between bunker silos. | 2011 | 21447012 |
| clinical and microbiologic outcomes in trauma patients treated for stenotrophomonas maltophilia ventilator-associated pneumonia. | to determine clinical and microbiologic plus clinical success rates in critically ill trauma patients who received treatment for stenotrophomonas maltophilia ventilator-associated pneumonia (vap). | 2011 | 21449623 |
| [the microbiology laboratory's contribution to the surveillance and control of outbreaks caused by nonfermentative gram-negative bacilli]. | pseudomonas aeruginosa, acinetobacter baumannii and stenotrophomonas maltophilia are, among others, the most important nonfermentative gram-negative bacilli within hospitals. these organisms are able to cause different types of nosocomial infections in predisposed patients, and p. aeruginosa and a. baumannii in particular may cause extensive clonal outbreaks and complex situations in which sporadic isolates may coexist with some epidemic strains. some common features are their intrinsic resistan ... | 2011 | 21458710 |
| gastrointestinal selective capacity of doripenem, meropenem, and imipenem for carbapenem-resistant gram-negative bacilli in treated patients with pneumonia. | 2011 | 21460501 | |
| causative pathogen dictates optimal duration of antimicrobial therapy for ventilator-associated pneumonia in trauma patients. | recent ventilator-associated pneumonia (vap) guidelines recommend considering abbreviated therapy in patients with non-pseudomonas aeruginosa vap if clinical signs resolve. however, using an arbitrary day cutoff or clinical signs can be suboptimal for some, especially multiply injured patients, resulting in relapse and/or antibiotic resistance. previously, we showed that repeat bronchoalveolar lavage (bal) could guide antimicrobial duration for community-acquired vap in trauma patients. the purp ... | 2011 | 21463773 |
| chronic pulmonary infection with stenotrophomonas maltophilia and lung function in patients with cystic fibrosis. | the clinical consequences of chronic stenotrophomonas maltophilia infection in cystic fibrosis (cf) patient are still unclear. | 2011 | 21463972 |
| stenotrophomonas maltophilia in cystic fibrosis: guilty or innocent? | 2011 | 21471053 | |
| induction of aromatic ring: cleavage dioxygenases in stenotrophomonas maltophilia strain kb2 in cometabolic systems. | stenotrophomonas maltophilia kb2 is known to produce different enzymes of dioxygenase family. the aim of our studies was to determine activity of these enzymes after induction by benzoic acids in cometabolic systems with nitrophenols. we have shown that under cometabolic conditions kb2 strain degraded 0.25-0.4 mm of nitrophenols after 14 days of incubation. simultaneously degradation of 3 mm of growth substrate during 1-3 days was observed depending on substrate as well as cometabolite used. fro ... | 2011 | 21475727 |
| changes in bacterial flora of japanese cabbage during growth and potential source of flora. | bacterial flora of cabbage were identified and enumerated during various stages of growth, and the potential sources of contamination in the field were determined. bacterial counts increased from below the level of detection (2.4 log cfu/g) on seeds to 2.5 to 5.7 log cfu/g on seedlings. after transplanting, the counts of mesophilic aerobic bacteria on leaves decreased and then increased to 5.7 log cfu/g on outer leaves, 5.0 log cfu/g on middle leaves, and 3.0 log cfu/g on inner leaves at the har ... | 2011 | 21477482 |
| diversity of culturable chitinolytic bacteria from rhizospheres of agronomic plants in japan. | a total of 100 isolates of chitinolytic bacteria were obtained from the rhizospheres of various agronomic plants, and the 16s rrna gene sequences of these isolates were determined. phylogenetic analyses revealed that 81 isolates belonged to the classes betaproteobacteria (39 isolates) and gammaproteobacteria (42 isolates). of the remaining 19 isolates, 16 belonged to the phylum firmicutes. clustering analysis identified 6 and 3 operational taxonomic units (otus) in gammaproteobacteria and betapr ... | 2011 | 21487197 |
| pathogen and autoantigen homologous regions within the cystic fibrosis transmembrane conductance regulator (cftr) protein suggest an autoimmune treatable component of cystic fibrosis. | the cystic fibrosis transmembrane conductance regulator (cftr) chloride channel provides the glutathione and hypochlorous acid necessary for bactericidal/viricidal actions. cftr mutations block these effects, diminishing pathogen defence and allowing extracellular pathogen accumulation, where antibody encounter is likely. kegg pathway analysis of the cftr interactome shows that cftr is involved in pathogen entry pathways and immune defence as well as in pathways relevant to comorbid conditions ( ... | 2011 | 21496124 |
| establishment of an arabinose-inducible system in stenotrophomonas maltophilia. | a pbbad22t-derived conditioned arabinose (ara)-inducible expression system was evaluated in stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). s. maltophilia cannot grow well when ara is the sole available carbon source. the induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xyle gene fusion construct, pbxyle, to monitor the expression of ... | 2011 | 21503736 |
| stenotrophomonas maltophilia: emerging disease patterns and challenges for treatment. | stenotrophomonas maltophilia is a ubiquitous organism associated with opportunistic infections. in the immunocompromised host, increasing prevalence and severity of illness is observed, particularly opportunistic bloodstream infections and pneumonia syndromes. in this article, the classification and microbiology are outlined, together with clinical presentation, outcomes and management of infections due to s. maltophilia. although virulence mechanisms and the genetic basis of antibiotic resistan ... | 2011 | 21504403 |
| revisited distribution of nonfermenting gram-negative bacilli clinical isolates. | nonfermenting gram-negative bacilli (nf-gnb) are ubiquitous environmental opportunistic bacteria frequently misidentified by conventional phenotypic methods. the aim of this study was to determine the distribution of nf-gnb species by 16 s rrna gene sequencing (used as reference method) and to compare performances of biochemical tests and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof-ms). from nine french hospitals, 188 nf-gnb isolates (except p. aerugin ... | 2011 | 21509476 |
| immunoassay method to check the flagellin mediated binding of stenotrophomonas maltophilia to polystyrene. | plate count and spectrophotometric methods have been used to asses the ability of an organism to attach to different surfaces and form biofilms. in the present study we report a highly sensitive, specific and quick method to check the role of flagellin in bacterial adhesion to polystyrene. flagellin from stenotrophomonas maltophilia showed high affinity for polystyrene (p < 0.05), which decreased on pretreatment offlagellin with anti-flagellin in a dilution dependent manner. in an enzyme immunoa ... | 2011 | 21513218 |
| microbial isolation and emergence of antimicrobial resistance associated with tigecycline usage. | background: with the increasing experience of tigecycline usage, its ecological impact on microorganisms raises concerns but remains unknown. we aimed to analyze the difference in microorganisms isolated before, during, and after tigecycline usage and their susceptibility to antimicrobial agents. methods: between july 2008 and august 2009, 66 patients who received tigecycline monotherapy for more than 2 days at a taiwan medical center were enrolled. antimicrobial susceptibility testing was perfo ... | 2011 | 21524975 |
| degradation of cyhalofop-butyl (cyb) by pseudomonas azotoformans strain qdz-1 and cloning of a novel gene encoding cyb-hydrolyzing esterase. | cyhalofop-butyl (cyb) is a widely used aryloxyphenoxy propanoate (aopp) herbicide for control of grasses in rice fields. five cyb-degrading strains were isolated from rice field soil and identified as agromyces sp., stenotrophomonas sp., aquamicrobium sp., microbacterium sp., and pseudomonas azotoformans ; the results revealed high biodiversity of cyb-degrading bacteria in rice soil. one strain, p. azotoformans qdz-1, degraded 84.5% of 100 mg l(-1) cyb in 5 days of incubation in a flask and util ... | 2011 | 21534595 |
| stenotrophomonas maltophilia strains replicate and persist in the murine lung, but to significantly different degrees. | the environmental bacterium stenotrophomonas maltophilia is increasingly described as a multidrug-resistant pathogen of humans, being associated with pneumonia, among other things. but, the degree to which s. maltophilia is capable of replicating in a mammalian host has been an issue of controversy. using a model of intranasal inoculation into adult a/j mice, we now document that s. maltophilia strain k279a, the clinical isolate of s. maltophilia whose complete genome sequence was recently deter ... | 2011 | 21546584 |
| adhesion mechanisms of plant-pathogenic xanthomonadaceae. | the family xanthomonadaceae is a wide-spread family of bacteria belonging to the gamma subdivision of the gram-negative proteobacteria, including the two plant-pathogenic genera xanthomonas and xylella, and the related genus stenotrophomonas. adhesion is a widely conserved virulence mechanism among gram-negative bacteria, no matter whether they are human, animal or plant pathogens, since attachment to the host tissue is one of the key early steps of the bacterial infection process. bacterial att ... | 2011 | 21557058 |
| complicated features in a young child with influenza b virus pneumonia and co-infection with stenotrophomonas maltophilia. | a 3·5-year-old child with influenza b virus pneumonia developed pneumomediastinum and subcutaneous emphysema on the 3rd day of illness. bronchoscopy demonstrated obstruction of the left main bronchus by mucopurulent sputum. culture of the broncho-alveolar lavage yielded stenotrophomonas maltophilia. after the respiratory complications resolved (11 days), the patient developed neurological symptoms and was diagnosed as acute disseminated encephalomyelitis (adem). stenotrophomonas maltophilia was ... | 2011 | 21575322 |
| predictors of catheter-related gram-negative bacilli bacteraemia among cancer patients. | clin microbiol infect abstract: gram-negative bacillary bacteraemia (gnb) is associated with high morbidity and mortality among cancer patients. we conducted this study to determine the risk factors that may predict the catheter as the source of gnb in cancer patients. from july 2005 to december 2006 all 266 cancer patients with gnb and central venous catheters (cvcs) at the university of texas m. d. anderson cancer centre in houston, were classified as catheter-related bloodstream infection (cr ... | 2011 | 21595787 |
| risk factors and clinical characteristics of stenotrophomonas maltophilia infections in neonates. | background: the aim of this study was to review the risk factors and clinical, bacteriological, and epidemiological characteristics of stenotrophomonas maltophilia infections in our neonatal intensive care unit. methods: a retrospective matched case-control study was performed by comparing 23 cases of s maltophilia with 45 controls to identify the potential risk factors. to identify the case patients, the admission and medical records of patients in the neonatal intensive care unit and records f ... | 2011 | 21606009 |
| case report: spontaneous stenotrophomonas maltophilia keratitis in a diabetic patient. | objectives:: to report a rare case of spontaneous stenotrophomonas maltophilia keratitis in a diabetic patient. methods:: a case report of this rare ulcerative keratitis case with an extensive review of the literature. results:: the patient's corneal infiltrate was successfully cultured and a rare ocular organism identified. the patient's presentation and course and the response to empiric treatment are discussed. conclusions:: consideration of the antibiotic combination chosen here can be consi ... | 2011 | 21617538 |
| intragenomic and intraspecific heterogeneity of the 16s rrna gene in seven bacterial species from the respiratory tract of cystic fibrosis patients assessed by pcr-temporal temperature gel electrophoresis. | 16s rrna gene-based cultivation-independent methods are increasingly used to study the diversity of microbiota during health and disease. one bias of these methods is the variability of 16s rrna gene that may exist among strains of a same species (intraspecific heterogeneity) or between rrs copies in a genome (intragenomic heterogeneity). we evaluated the level of intraspecific and intragenomic 16s rdna variability in seven species frequently encountered in respiratory tract samples in cystic fi ... | 2011 | 21621347 |
| a novel highly charged exopolysaccharide produced by two strains of stenotrophomonas maltophilia recovered from patients with cystic fibrosis. | stenotrophomonas maltophilia is a non-fermenting gram-negative microorganism capable of causing chronic pulmonary infection in cystic fibrosis patients and its ability to form biofilms on polystyrene and glass surfaces, as well as on cystic fibrosis-derived bronchial epithelial ib3-i cells was recently demonstrated. the latter evidence might explain the power of s. maltophilia to produce persistent lung infections, despite intensive antibiotic treatment. in addition to being important components ... | 2011 | 21636078 |
| an unknown complication of peripherally inserted central venous catheter in a patient with ventricular assist device. | we report an unknown complication of peripherally inserted central venous catheter in a patient with ventricular assist device. this rare complication led to the failure of the right ventricular assist device, which could be detrimental in patients with dilated cardiomyopathy. | 2011 | 21636933 |
| construction of river model biofilm for assessing pesticide effects. | due to the high importance of biofilms on river ecosystems, assessment of pesticides' adverse effects is necessary but is impaired by high variability and poor reproducibility of both natural biofilms and those developed in the laboratory. we constructed a model biofilm to evaluate the effects of pesticides, consisting in cultured microbial strains, pedobacter sp. 7-11, aquaspirillum sp. t-5, stenotrophomonas sp. 3-7, achnanthes minutissima n71, nitzschia palea n489, and/or cyclotella meneghinia ... | 2011 | 20422166 |
| screening for endophytic nitrogen-fixing bacteria in brazilian sugar cane varieties used in organic farming and description of stenotrophomonas pavanii sp. nov. | a gram-negative, rod-shaped, non-spore-forming and nitrogen-fixing bacterium, designated icb 89(t), was isolated from stems of a brazilian sugar cane variety widely used in organic farming. 16s rrna gene sequence analysis revealed that strain icb 89(t) belonged to the genus stenotrophomonas and was most closely related to stenotrophomonas maltophilia lmg 958(t), stenotrophomonas rhizophila lmg 22075(t), stenotrophomonas nitritireducens l2(t), [pseudomonas] geniculata atcc 19374(t), [pseudomonas] ... | 2011 | 20495025 |
| stenotrophomonas daejeonensis sp. nov., isolated from sewage. | a gram-stain-negative, motile, aerobic bacterial strain, designated mj03(t), was isolated from sewage and was characterized taxonomically by using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strain mj03(t) belongs to the family xanthomonadaceae, class gammaproteobacteria, and was related most closely to stenotrophomonas acidaminiphila amx 19(t) (97.9 % sequence similarity), stenotrophomonas humi r-32729(t) (97.1 %), stenotrophomonas nitritireducens l2(t) (96. ... | 2011 | 20400671 |
| tertiary structure-related activity of tick defensin (persulcatusin) in the taiga tick, ixodes persulcatus. | defensins are small cysteine-rich cationic proteins found in both vertebrates and invertebrates constituting the front line of host innate immunity. to examine the importance of the tertiary structure of tick defensin in its antimicrobial activity, we synthesized two types of the peptides with tertiary structure or primary one on basis of the information of the sequence in the defensin originated from the taiga tick, ixodes persulcatus. chemically synthesized peptides were used to investigate th ... | 2011 | 20596886 |
| microbial etiologies of hospital-acquired bacterial pneumonia and ventilator-associated bacterial pneumonia. | hospital-acquired bacterial pneumonia (habp) and ventilator-associated bacterial pneumonia (vabp) can be caused by a wide variety of bacteria that originate from the patient flora or the health care environment. we review the medical and microbiology literature and the results of the sentry antimicrobial surveillance program (1997-2008) to establish the pathogens most likely to cause habp or vabp. in all studies, a consistent 6 organisms (staphylococcus aureus [28.0%], pseudomonas aeruginosa [21 ... | 2010 | 20597676 |
| isolation, characterization and phylogeny of sponge-associated bacteria with antimicrobial activities from brazil. | bacteria associated with marine sponges represent a rich source of bioactive metabolites. the aim of this study was to isolate and characterize bacteria with antimicrobial activities from brazilian sponges. a total of 158 colony-forming units were isolated from nine sponge species. among these, 12 isolates presented antimicrobial activities against pathogenic bacteria. based on comparative sequence analysis of their 16s rrna genes, the sponge-associated bacterial strains could be subdivided into ... | 2010 | 20600863 |
| [analysis of acquired resistance genes in stenotrophomonas maltophilia]. | stenotrophomonas maltophilia is a gram-negative bacillus and a nosocomial pathogen in immunocompromised patients. trimethoprim/sulfamethoxazole (tmp/smx) is the drug of choice for treating s. maltophilia infection; however, resistance to tmp/smx is increasing. in this study, we investigated the relationship between the incidence of tmp/smx resistance and the presence of sul genes and mobile elements. | 2010 | 20603591 |
| newer antibacterial agents and their potential role in cystic fibrosis pulmonary exacerbation management. | pulmonary exacerbations in cystic fibrosis (cf) are frequent events and account for a substantial proportion of the burden of morbidity and mortality in this disease. antibacterial therapies to treat pulmonary exacerbations are instituted empirically and are individualized based on both patient factors (severity of exacerbation, frequency of exacerbation, recent courses of anti-infectives) and pathogen factors (previously isolated pathogens and in vitro predicted susceptibilities). however, the ... | 2010 | 20605846 |
| diffusion measurements inside biofilms by image-based fluorescence recovery after photobleaching (frap) analysis with a commercial confocal laser scanning microscope. | research about the reactional and structural dynamics of biofilms at the molecular level has made great strides, owing to efficient fluorescence imaging methods in terms of spatial resolution and fast acquisition time but also to noninvasive conditions of observation consistent with in situ biofilm studies. in addition to conventional fluorescence intensity imaging, the fluorescence recovery after photobleaching (frap) module can now be routinely implemented on commercial confocal laser scanning ... | 2010 | 20639359 |
| [investigation of distribution of bacteria and fungi in severe acute pancreatitis]. | to investigate the spectrum of bacteria and fungi in different sites in severe acute pancreatitis (sap). | 2010 | 20646657 |
| post-antibiotic effect (pae) of moxifloxacin in multidrug-resistant stenotrophomonas maltophilia. | 2010 | 20674284 | |
| rapid detection of pseudomonas aeruginosa from positive blood cultures by quantitative pcr. | pseudomonas aeruginosa is responsible for numerous bloodstream infections associated with severe adverse outcomes in case of inappropriate initial antimicrobial therapy. the present study was aimed to develop a novel quantitative pcr (qpcr) assay, using ecfx as the specific target gene, for the rapid and accurate identification of p. aeruginosa from positive blood cultures (bcs). | 2010 | 20684778 |
| [biofilm on a metal surface as a factor of microbial corrosion]. | main attention was given in the present review to the research methods, phases of biofilm's forming, exopolymer compounds of bacteria as main biofilm forming factor. a microbial corrosion as a result of interaction between the biofilm and metal surface was considered. the interaction was displayed in biomineralization. the future trends of biofilms study were bound with research of their architecture. that architecture was determined by the structure and function of biofilms compounds: biopolyme ... | 2010 | 20695231 |
| prevalence of antimicrobial resistance among gram-negative isolates in an adult intensive care unit at a tertiary care center in saudi arabia. | patients in the icu have encountered an increasing emergence and spread of antibiotic-resistant pathogens. we examined patterns of antimicrobial susceptibility in gram-negative isolates to commonly used drugs in an adult icu at a tertiary care hospital in riyadh, saudi arabia. | 2010 | 20697174 |
| [isolation, identification, and degrading characteristics of a high-efficient pyrene-degrading bacterial strain]. | by using selective enrichment culture with pyrene as the sole carbon source, a pyrene-degrading bacterial strain zq5 was isolated from the oil-contaminated soil in shenfu irrigation area. the strain was identified as stenotrophomonas sp., based on its morphological, physiological, and biochemical characteristics, and similarity identification of 16s rdna sequence. the pyrene-degrading characteristics and the effects of culture condition on the degrading efficiency of the strain were investigated ... | 2010 | 20707122 |
| infections occurring during extracorporeal membrane oxygenation use in adult patients. | the application of extracorporeal membrane oxygenation in adults has been increasing, but infections occurring during extracorporeal membrane oxygenation use are rarely described. | 2010 | 20708754 |
| [identifying bacteria using a matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometer. comparison with routine methods used in clinical microbiology laboratories]. | the methods routinely used for bacterial identification in clinical microbiology laboratory, although miniaturized and automated, are still based on the same basic principles as classical identification methods. nevertheless, technological advances are emerging which could modify these routine methods. we report a comparative study between conventional identification methods and mass spectrometry maldi-tof (ms maldi-tof) for bacterial identification in the clinical microbiology laboratory. | 2010 | 20409613 |
| structure, function, and insights into the biosynthesis of a head-to-head hydrocarbon in shewanella oneidensis strain mr-1. | a polyolefinic hydrocarbon was found in nonpolar extracts of shewanella oneidensis mr-1 and identified as 3,6,9,12,15,19,22,25,28-hentriacontanonaene (compound i) by mass spectrometry, chemical modification, and nuclear magnetic resonance spectroscopy. compound i was shown to be the product of a head-to-head fatty acid condensation biosynthetic pathway dependent on genes denoted as ole (for olefin biosynthesis). four ole genes were present in s. oneidensis mr-1. deletion of the entire oleabcd ge ... | 2010 | 20418444 |
| case report 6: candidaemia in a high risk liver transplant recipient. | 2010 | 20591016 | |
| aerosolized amikacin and oral clarithromycin to eradicate mycobacterium abscessus in a patient with cystic fibrosis: an 8-year follow-up. | 2010 | 20503290 | |
| structure of stenotrophomonas maltophilia feoa complexed with zinc: a unique prokaryotic sh3-domain protein that possibly acts as a bacterial ferrous iron-transport activating factor. | iron is vital to the majority of prokaryotes, with ferrous iron believed to be the preferred form for iron uptake owing to its much better solubility. the major route for bacterial ferrous iron uptake is found to be via an feo (ferrous iron-transport) system comprising the three proteins feoa, feob and feoc. although the structure and function of feob have received much attention recently, the roles played by feoa and feoc have been little investigated to date. here, the tertiary structure of fe ... | 2010 | 20516589 |
| clinical characteristics of stenotrophomonas maltophilia infection in hematopoietic stem cell transplantation recipients: a single center experience. | recipients of hematopoietic stem-cell transplantation (hsct) are at high risk for infections caused by stenotrophomonas maltophilia. | 2010 | 20425134 |
| isolation and characterization of a virulent bacteriophage ab1 of acinetobacter baumannii. | acinetobacter baumannii is an emerging nosocomial pathogen worldwide with increasing prevalence of multi-drug and pan-drug resistance. a. baumannii exists widely in natural environment, especially in health care settings, and has been shown difficult to be eradicated. bacteriophages are often considered alternative agent for controlling bacterial infection and contamination. in this study, we described the isolation and characterization of one virulent bacteriophage ab1 capable of specifically i ... | 2010 | 20426877 |
| colistin susceptibility testing: evaluation of reliability for cystic fibrosis isolates of pseudomonas aeruginosa and stenotrophomonas maltophilia. | antibiotic susceptibility methods that are commonly used to test bacterial isolates from patients with cystic fibrosis are of uncertain reliability for the polymyxins. to assess the reliability of four standard testing methods, this pilot study used a challenge set that included polymyxin-resistant isolates of pseudomonas aeruginosa and stenotrophomonas maltophilia. | 2010 | 20430789 |
| chemical composition of endemic scorzonera sandrasica and studies on the antimicrobial activity against multiresistant bacteria. | the present study describes the antimicrobial activity and chemical composition of scorzonera sandrasica hartvig et strid (family asteraceae), endemic to turkey. the antimicrobial activity of the hexane, chloroform, ethyl acetate, and ethanol extracts of the aerial parts of s. sandrasica was evaluated against microorganisms, including multiresistant bacteria, using a paper disc diffusion method. the chemical composition of the chloroform extract of the plant was determined by gas chromatography ... | 2010 | 20438328 |
| comparison of different antimicrobial susceptibility testing methods for stenotrophomonas maltophilia and results of synergy testing. | accurate determination of resistance is important to ensure appropriate antimicrobial therapy in stenotrophomonas maltophilia infections. this study was undertaken to evaluate the susceptibility results obtained by disc diffusion, e-test, phoenix system, and reference agar dilution method and also to evaluate the in vitro activity of various antimicrobial combinations against multidrug-resistant s. maltophilia. susceptibilities to several antimicrobial agents were determined by agar dilution, di ... | 2010 | 20449623 |
| [investigation of the in vitro antibacterial effects of statins]. | it was previously shown that statins have anti-inflammatory, immunomodulatory and anti-oxidant effects. this study was aimed to investigate the in-vitro antibacterial effects of simvastatin and atorvastatin. in this study antibacterial activity of the statins were tested against 16 methicillin-susceptible stophylococcus aureus (mssa), 16 methicillin-resistant s.aureus (mrsa), 16 methicillin-resistant coagulase-negative staphylococcus (mrcons), 9 vancomycin-susceptible enterococcus faecium, 7 van ... | 2010 | 20455414 |
| genetic engineering of stenotrophomonas strain yc-1 to possess a broader substrate range for organophosphates. | in this work, stenotrophomonas sp. strain yc-1, a native soil bacterium that produces methyl parathion hydrolase (mph), was genetically engineered to possess a broader substrate range for organophosphates (ops). a surface anchor system derived from the truncated ice nucleation protein (inpnc) from pseudomonas syringae was used to target organophosphorus hydrolase (oph) onto the cell surface of strain yc-1, reducing the potential substrate uptake limitation. the surface localization of inpnc-oph ... | 2010 | 20455565 |
| comparison of e-test and disc diffusion methods for the in vitro evaluation of the antimicrobial activity of colistin in multi-drug resistant gram-negative bacilli. | to study susceptibility of clinical isolates of multi-drug resistant (mdr) gram-negative bacilli to colistin by minimum inhibitory concentration (mic) determination using etest, and compare this with their susceptibility measured by the disc diffusion (dd) method. | 2010 | 20464039 |
| antimicrobial activity of the green tea polyphenol (-)-epigallocatechin-3-gallate (egcg) against clinical isolates of stenotrophomonas maltophilia. | stenotrophomonas maltophilia is increasingly recognised as an important nosocomial pathogen. treatment options are limited due to intrinsic resistance to many antibiotics as well as concerns over toxicity of the mainstay of treatment, co-trimoxazole. epigallocatechin-3-gallate (egcg), the major catechin found in green tea, has been shown to have antimicrobial effects against a number of bacterial pathogens. we evaluated the in vitro activity of this compound against 40 clinical isolates of s. ma ... | 2010 | 20472404 |
| isolation and characterization of butachlor-catabolizing bacterial strain stenotrophomonas acidaminiphila js-1 from soil and assessment of its biodegradation potential. | isolation, characterization and assessment of butachlor-degrading potential of bacterial strain js-1 in soil. | 2010 | 20477958 |
| degradation of microcystin-lr and rr by a stenotrophomonas sp. strain ems isolated from lake taihu, china. | a bacterial strain ems with the capability of degrading microcystins (mcs) was isolated from lake taihu, china. the bacterium was tentatively identified as a stenotrophomonas sp. the bacterium could completely consume mc-lr and mc-rr within 24 hours at a concentration of 0.7 microg/ml and 1.7 microg/ml, respectively. the degradation of mc-lr and mc-rr by ems occurred preferentially in an alkaline environment. in addition, mlra gene involved in the degradation of mc-lr and mc-rr was detected in e ... | 2010 | 20479990 |
| stenotrophomonas maltophilia flagellin induces a compartmentalized innate immune response in mouse lung. | intranasal (i.n.) instillation of different amounts of purified stenotrophomonas maltophilia flagellin preparation (1, 5 and 15 microg) in balb/c mice stimulated a transient innate immune response in the lungs. this was characterized by infiltration of different kinds of leukocytes (neutrophils, monocytes and lymphocytes), production of various inflammatory mediators (tumour necrosis factor alpha, interleukin 1 beta, interleukin 10, nitric oxide, myeloperoxidase and malondialdehyde) and activate ... | 2010 | 20488935 |
| stenotrophomonas maltophilia: significant contemporary hospital pathogen - review. | stenotrophomonas maltophilia (sm) plays an important role as an opportunistic pathogen in immunocompromised individuals. the growing detection rates of this bacterium in hospitalized patients are associated with the invasiveness of therapeutic and diagnostic procedures and the selection pressure of antibiotic therapy. a broad range of infections that can be caused by sm is frequently bound to biofilm. the high level of intrinsic resistance to many unrelated antibiotics and increasing acquired re ... | 2010 | 20526844 |
| analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry. | comprehensive two-dimensional gas chromatography (gcxgc) offers an interesting tool for profiling bacterial fatty acids. flow modulated gcxgc using a commercially available system was evaluated, different parameters such as column flows and modulation time were optimized. the method was tested on bacterial fatty acid methyl esters (bames) from stenotrophomonas maltophilia lmg 958t by using parallel flame ionization detector (fid)/mass spectrometry (ms). the results are compared to data obtained ... | 2010 | 20527679 |
| a giant family of short palindromic sequences in stenotrophomonas maltophilia. | the genome of stenotrophomonas maltophilia is peppered with palindromic elements called smag (stenotrophomonas maltophilia gtag) because they carry at one terminus the tetranucleotide gtag. the repeats are species-specific variants of the superfamily of repetitive extragenic palindromes (reps), dna sequences spread in the intergenic space in many prokaryotic genomes. the genomic organization and the functional features of smag elements are described herein. a total of 1650 smag elements were ide ... | 2010 | 20528935 |
| the role of stenotrophomonas maltophilia in refractory chronic rhinosinusitis. | stenotrophomonas maltophilia is a multidrug-resistant gram-negative bacillus that has been implicated in serious nosocomial infections. this microbe has also been isolated from sinus cultures in refractory chronic rhinosinusitis (crs). the goal of this study was to elucidate the implications of s. maltophilia-positive cultures in the setting of crs. the objectives of the study were (1) to define clinical and microbiological characteristics and (2) to assess management strategy and overall outcom ... | 2010 | 20537287 |
| in vitro synergistic/additive activity of levofloxacin with meropenem against stenotrophomonas maltophilia. | synergy testing of levofloxacin and meropenem by etest and time-kill assay (tka) was performed against 30 genetically unique clinical stenotrophomonas maltophilia isolates. synergy was demonstrated in 18/30 (60%) isolates by etest and in 13/30 (43%) by tka; the remaining isolates were indifferent. methods showed agreement for 25/30 (83%) of isolates. | 2010 | 20542209 |
| analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry. | comprehensive two-dimensional gas chromatography (gcxgc) offers an interesting tool for profiling bacterial fatty acids. flow modulated gcxgc using a commercially available system was evaluated, different parameters such as column flows and modulation time were optimized. the method was tested on bacterial fatty acid methyl esters (bames) from stenotrophomonas maltophilia lmg 958(t) by using parallel flame ionization detector (fid)/mass spectrometry (ms). the results are compared to data obtaine ... | 2010 | 20546760 |
| evaluation of genetic and functional diversity of stenotrophomonas isolates from diverse effluent treatment plants. | in this study, the samples were collected from nine etps and soil contaminated with petroleum products. the genetic diversity of 30 stenotrophomonas isolates was demonstrated by phylogenetic analysis of their 16s rrna gene nucleotide sequences, and randomly amplified polymorphic dna (rapd) analysis supplemented with in silico signature and restriction enzyme (res - alui, bfai, dpnii, haeiii, rsai and tru9i) digestion analyses. genetic diversity based on nucleotide sequence data revealed distinct ... | 2010 | 20554196 |
| [endocarditis due to stenotrophomonas maltophilia and enterobacter cloacae]. | 2010 | 20570073 | |
| clinical profile of adult cystic fibrosis patients with frequent epidemic clones of pseudomonas aeruginosa. | earlier reports suggested that pseudomonas aeruginosa frequent epidemic clones circulating in cystic fibrosis (cf) centres had increased virulence. however, recent data show no consistent associations with virulence, and suggest attenuation of virulence in chronic infection. changes to infection control programmes in relation to frequent epidemic clones should be based on their frequency, virulence across all age groups and mode of acquisition. the australian epidemic strain-1 (aes-1) (or the me ... | 2010 | 20573059 |
| [interpretive reading of the non-fermenting gram-negative bacilli antibiogram]. | among non-fermenting gram-negative rods, the most clinically important species are pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia, which are frequently multiresistant. p. aeruginosa resistance to beta-lactams depends on the production of chromosomal and plasmid-mediated beta-lactamases, altered permeability (loss of oprd porin is related to carbapenem-resistance) and active efflux systems, particularly mexab-oprm. in aminoglycoside resistant strains, the main m ... | 2010 | 20579775 |
| [a sensitive, specific and predictive isolation medium developed for stenotrophomonas maltophilia study in healthcare settings]. | stenotrophomonas maltophilia (smalto) is a prominent nosocomial pathogen, commonly isolated in the hospital environment. multiple smalto nosocomial outbreaks have been linked to contaminated water sources. this study aimed to develop a medium able to ease healthcare environment smalto isolation. | 2010 | 19892479 |
| [stenotrophomonas maltophilia and pseudomonas aeruginosa water-associated microbiologic risk assessment in amiens' university hospital centre]. | pseudomonas aeruginosa (psa) and stenotrophomonas maltophilia (smalto) are major opportunistic waterborne pathogens causing hospital-acquired infections. this study aimed to assess the biocontamination level of cold water used in amiens' university hospital wards, from march to june 2008. | 2010 | 19892487 |
| continuous 2-keto-l-gulonic acid fermentation by mixed culture of ketogulonicigenium vulgare dsm 4025 and bacillus megaterium or xanthomonas maltophilia. | the fermentation process of 2-keto-l-gulonic acid (2kga) from l-sorbose was developed using a two-stage continuous fermentation system. the mixed culture of ketogulonicigenium vulgare dsm 4025 and bacillus megaterium dsm 4026 produced 90 g/l of 2kga from 120 g/l of l-sorbose at the dilution rate of 0.01 h(-1) in a single-stage continuous fermentation process. but after the production period was beyond 150 h, the significant decrease of 2kga productivity was observed. when the non-spore forming b ... | 2010 | 19902207 |
| beverages obtained from soda fountain machines in the u.s. contain microorganisms, including coliform bacteria. | ninety beverages of three types (sugar sodas, diet sodas and water) were obtained from 20 self-service and 10 personnel-dispensed soda fountains, analyzed for microbial contamination, and evaluated with respect to u.s. drinking water regulations. a follow-up study compared the concentration and composition of microbial populations in 27 beverages collected from 9 soda fountain machines in the morning as well as in the afternoon. ice dispensed from these machines was also examined for microbial c ... | 2010 | 19926155 |
| multidrug resistance in clinical isolates of stenotrophomonas maltophilia: roles of integrons, efflux pumps, phosphoglucomutase (spgm), and melanin and biofilm formation. | integrons, efflux pumps, phosphoglucomutase (spgm), and melanin and biofilm formation were investigated in 40 multidrug-resistant (mdr) and 30 non-mdr stenotrophomonas maltophilia isolates recovered from patients treated at national taiwan university hospital (taipei, taiwan). class 1 integrons were clearly associated with multidrug resistance. sequencing data revealed that aminoglycoside-modifying genes occurred most frequently in the integron and disclosed a new bla(imp-8)/aac6-ii/aada5 gene c ... | 2010 | 19926255 |