Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| the diversity and antifungal susceptibility of the yeasts isolated from coconut water and reconstituted fruit juices in brazil. | the aims of this study were to characterise the yeasts present in the reconstituted fruit juices and coconut water extracted with "coconut machines", both collected from commercial outlets in a brazilian city, and to investigate the antifungal resistance of isolates from these beverages that were able to grow at 37°c. the yeast population counts in the coconut water samples ranged from 1.7 to >6.5logcfu/ml, and in the reconstituted fruit juices, the counts ranged from 1.5 to >5.5logcfu/ml. aureo ... | 2012 | 23290225 |
| calcineurin is required for pseudohyphal growth, virulence, and drug resistance in candida lusitaniae. | candida lusitaniae is an emerging fungal pathogen that infects immunocompromised patients including hiv/aids, cancer, and neonatal pediatric patients. though less prevalent than other candida species, c. lusitaniae is unique in its ability to develop resistance to amphotericin b. we investigated the role of the calcium-activated protein phosphatase calcineurin in several virulence attributes of c. lusitaniae including pseudohyphal growth, serum survival, and growth at 37°c. we found that calcine ... | 2012 | 22952924 |
| candida non-albicans versus candida albicans fungemia in the non-neonatal pediatric population. | non-albicans candida (nac) species have been implicated as major pathogens in patients with hospital-acquired candidemia. few studies have investigated the impact of nac fungemia among pediatric patients outside of the neonatal age group. | 2011 | 21372750 |
| [scientific evidence supporting the use of micafungin in the treatment of invasive candidiasis]. | micafungin is a semisynthetic lipopeptide developed from coleophoma empetri, which blocks the synthesis of ß-1,3-d-glucan, an essential component of the fungal wall, though non-competitive inhibition of ß-1,3-d-glucan synthetase. micafungin is a dose-dependent candidacidal agent with excellent in vitro efficacy against most candida spp. including species resistant to amphotericin b, such as candida lusitaniae, several azoles, such as c. glabrata or c. krusei, and isolates not susceptible to othe ... | 2011 | 21420572 |
| a two-step cloning-free pcr-based method for the deletion of genes in the opportunistic pathogenic yeast candida lusitaniae. | we describe a new cloning-free strategy to delete genes in the opportunistic pathogenic yeast candida lusitaniae. we first constructed two ura3 ? strains in c. lusitaniae for their use in transformation experiments. one was deleted for the entire ura3 coding sequence; the other possessed a partial deletion within the coding region, which was used to determine the minimum amount of homology required for efficient homologous recombination by double crossing-over of a linear dna fragment restoring ... | 2011 | 21456057 |
| in vitro antifungal susceptibilities of candida isolates from patients with invasive candidiasis in kuala lumpur hospital, malaysia. | the in vitro antifungal susceptibilities of 159 clinical isolates of candida species from patients with invasive candidiasis in kuala lumpur hospital, malaysia, were determined against amphotericin b, fluconazole, voriconazole, itraconazole and caspofungin. the most common species were candida albicans (71 isolates), candida parapsilosis (42 isolates), candida tropicalis (27 isolates) and candida glabrata (12 isolates). the susceptibility tests were carried out using an e-test. the mic breakpoin ... | 2011 | 21459913 |
| development of echinocandin resistance in clavispora lusitaniae during caspofungin treatment. | clavispora lusitaniae is an opportunistic human pathogen responsible for 0.6 to 2% of candidemia. this species is intrinsically susceptible to echinocandins. nevertheless, in this study, development of echinocandin resistance in c. lusitaniae isolates was observed during caspofungin treatment. this resistance resulted from missense mutation in the echinocandin target fks1 gene. | 2011 | 21490186 |
| extensive dna-binding specificity divergence of a conserved transcription regulator. | the dna sequence recognized by a transcription regulator can be conserved across large evolutionary distances. for example, it is known that many homologous regulators in yeasts and mammals can recognize the same (or closely related) dna sequences. in contrast to this paradigm, we describe a case in which the dna-binding specificity of a transcription regulator has changed so extensively (and over a much smaller evolutionary distance) that its cis-regulatory sequence appears unrelated in differe ... | 2011 | 21498688 |
| posaconazole in human serum: a greater pharmacodynamic effect than predicted by the non-protein-bound serum concentration. | it is generally accepted that only the unbound fraction of a drug is pharmacologically active. posaconazole is an antifungal agent with a protein binding of 98-99%. taking into account the degree of protein binding, plasma levels in patients and mic-levels of susceptible strains; it can be assumed that the free concentration of posaconazole sometimes will be too low to exert the expected antifungal effect. the aim was therefore to test the activity of posaconazole in serum in comparison with tha ... | 2011 | 21502622 |
| enzymatic activity of yeasts isolated from the inflamed mammary secretion in dairy cows. | the aim of the study was to evaluate enzymatic activities of yeasts isolated from inflammatory mammary secretion. the yeasts isolated from cows with clinical and sub-clinical mastitis (134 strains) included: candida krusei (62 strains), candida kefyr (48 strains), candida lusitaniae (17 strains) and candida famata (7 strains). the api zym system was used containing substrates to assess 19 hydrolytic enzymes. substantial differences in the number and activity of hydrolyses were demonstrated in in ... | 2011 | 21528713 |
| epidemiology and microbiology of nosocomial pediatric candidemia at a northern indian tertiary care hospital. | the availability and aggressive use of chemotherapeutic and immunosuppressive agents as well as broad-spectrum antibacterial agents have created a large population of patients who are at increased risk of acquiring infections with fungal organisms, especially candida species. present work was undertaken to study the epidemiology and microbiology of candidemia and candida colonization in hospitalized children. a total of 323 suspected cases of septicemia were enrolled, of which blood culture from ... | 2011 | 21533904 |
| in vitro antifungal activity of phenothiazines and their combination with amphotericin b against different candida species. | candidiosis is a mycosis that is currently increasingly affecting the population in consequence of its frequency and the severity of its complications, especially among immunocompromised hosts. in this work, the in vitro anticandidal activities of two phenothiazines (ptzs), chlorpromazine (cpz) and trifluoperazine (tfp), and their combinations with amphotericin b (amb) were tested against 12 different candida strains representing 12 species (candida albicans, candida glabrata, candida guillermon ... | 2011 | 21605196 |
| microbial inactivation in cloudy apple juice by multi-frequency dynashock power ultrasound. | the study determined the efficacy of dynashock wave power ultrasound as an alternative processing technique for apple juice against a number of pathogenic and spoilage microorganisms. the effects of several implicit, intrinsic and extrinsic properties on the dynashock wave inactivation of the microorganisms were also investigated. results showed that acid adaptation increased the resistance of escherichia coli o157:h7 and salmonella spp. but decreased that of listeria monocytogenes. spoilage yea ... | 2011 | 21802974 |
| diversity of bacteria and yeast in the naturally fermented cotton seed and rice beverage produced by brazilian amerindians. | microorganisms associated with the fermentation of cotton seed and rice were studied using a combination of culture-dependent and -independent methods. samples of the cotton seed and rice beverage were collected every 8-áh during the fermentation process for analysis of the microbiota present over 48-áh. the lactic acid bacteria (lab) population reached values of approximately 8.0-álog-ácfu/ml. a total of 162 bacteria and 81 yeast isolates were identified using polyphasic methods. lab (lactobaci ... | 2011 | 21839389 |
| yeast communities associated with artisanal mezcal fermentations from agave salmiana. | the aims of this work were to characterize the fermentation process of mezcal from san luis potosi, m+®xico and identify the yeasts present in the fermentation using molecular culture-dependent methods (rflp of the 5.8s-its and sequencing of the d1/d2 domain) and also by using a culture-independent method (dgge). the alcoholic fermentations of two separate musts obtained from agave salmiana were analyzed. sugar, ethanol and major volatile compounds concentrations were higher in the first ferment ... | 2011 | 21681584 |
| proanthocyanidin-rich extracts from cranberry fruit ( vaccinium macrocarpon ait.) selectively inhibit the growth of human pathogenic fungi candida spp. and cryptococcus neoformans. | cranberry ( vaccinium macrocarpon ) has been shown in clinical studies to reduce infections caused by escherichia coli and other bacteria, and proanthocyanidins are believed to play a role. the ability of cranberry to inhibit the growth of opportunistic human fungal pathogens that cause oral, skin, respiratory, and systemic infections has not been well-studied. fractions from whole cranberry fruit were screened for inhibition of five candida species and cryptococcus neoformans , a causative ... | 2011 | 22066866 |
| identification and characterization of a novel legume-like lectin cdna sequence from the red marine algae gracilaria fisheri. | a legume-type lectin (l-lectin) gene of the red algae gracilaria fisheri (gfl) was cloned by rapid amplification of cdna ends (race). the full-length cdna of gfl was 1714 bp and contained a 1542 bp open reading frame encoding 513 amino acids with a predicted molecular mass of 56.5 kda. analysis of the putative amino acid sequence with ncbi-blast revealed a high homology (30-68%) with legume-type lectins (l-lectin) from griffithsia japonica, clavispora lusitaniae, acyrthosiphon pisum, tetraodon n ... | 2011 | 22116281 |
| usefulness of mcrapd for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by candida lusitaniae. | a case report of ventriculoperitoneal shunt infection caused by candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the course of antifungal treatment. seven isolates recovered subsequently from the cerebrospinal fluid were studied retrospectively. to confirm identity, isolates were typed using pulsed-field gel electrophoresis and melting curve of random amplified polymorphic dna (mcrapd). further, the ability t ... | 2011 | 21894550 |
| evaluation of luminex xtag fungal analyte-specific reagents for rapid identification of clinically relevant fungi. | invasive fungal infections (ifi) remain a serious threat to immunocompromised hosts. current diagnostic methods, including fungal culture and antigen detection, are slow and often lack specificity. rapid diagnostic tools with increased sensitivity and specificity could improve the care of patients with ifi. recently, luminex molecular diagnostics (toronto, canada) developed 23 analyte-specific reagents (asrs) for the detection of the most common clinically relevant fungi. this study's objective ... | 2011 | 21880976 |
| placental and fetal findings in intrauterine candida lusitaniae infection following in vitro fertilization and embryo transfer. | abstract intrauterine infection with non-albicans candida species is rare but can be catastrophic to the fetus. a subset of intrauterine infections with non-albicans candida species have occurred in women who have undergone in vitro fertilization and embryo transfer (ivf-et). we report a case of a 33 year-old healthy woman, pregnant with triplets by in-vitro fertilization, who experienced preterm premature rupture of membranes of fetus a at 16 weeks gestation and subsequently developed oligohy ... | 2011 | 21864122 |
| defining pheromone-receptor signaling in candida albicans and related asexual candida species. | candida albicans is an important human fungal pathogen in which sexual reproduction is under the control of the novel white-opaque switch. opaque cells are the mating-competent form, whereas white cells do not mate but can still respond to pheromones, resulting in biofilm formation. in this study, we first define the domains of the α-pheromone receptor ste2 that are necessary for signaling in both white and opaque forms. both cell states require the ic loop 3 (ic3) and the c-terminal tail of ste ... | 2011 | 21998194 |
| candida lusitaniae discitis after discogram in an immunocompetent patient. | discitis or epidural abscess after discogram is a rare but known complication. it is more commonly bacterial; however, fungal discitis has been previously reported in immunocompromised patients. the management of fungal discitis in immunocompetent patients is rarely presented or addressed in the literature. | 2011 | 22005083 |
| clavispora lusitaniae and chaetomium atrobrunneum as rare agents of cutaneous infection. | we describe the first case of cutaneous infection caused by chaetomium atrobrunneum and clavispora lusitaniae in a one-and-a-half-year-old boy with acute and severe inflammation around his left eyelid. he presented to our outpatient center with a 6-day history and previously ineffective antibacterial therapy. scanning electron microscopy (sem) revealed hyphae and spores were on the surface of the crusty exudates and also penetrated into it, and the microbiology study further showed their charact ... | 2010 | 20020214 |
| development and evaluation of a real-time quantitative pcr assay for detection and enumeration of yeasts of public health interest in dairy products. | yeast contamination is a problem in the food industry as a cause of spoilage. moreover, various species of yeasts are known to be capable of causing opportunistic infections in humans. we have developed a real-time quantitative pcr (qpcr) assay to directly detect and quantify nine emerging opportunistic yeast species (candida albicans, candida glabrata, candida parapsilosis, candida tropicalis, clavispora lusitaniae, filobasidiella neoformans, issatchenkia orientalis, trichosporon asahii, and tr ... | 2010 | 20223545 |
| in vitro susceptibility to seven antifungal agents of candida lusitaniae isolates from an italian university hospital. | 2010 | 20227998 | |
| in vitro activity of amphotericin b against candida lusitaniae clinical isolates. | 2010 | 20227999 | |
| multidrug resistant yeasts in synanthropic wild birds. | the aim of this study was to investigate the presence of multidrug resistant yeasts in the faeces of synanthropic wild birds from the bangsar suburb of kuala lumpur. | 2010 | 20307325 |
| [susceptibility to fluconazole and voriconazole of candida species isolated from intensive care units patients in medellin, colombia (2001-2007)]. | disseminated candidiasis is caused by different candida species and mainly affects immunocompromised patients and those hospitalized in intensive care units (icu). | 2010 | 20450982 |
| analysis of gene evolution and metabolic pathways using the candida gene order browser. | candida species are the most common cause of opportunistic fungal infection worldwide. recent sequencing efforts have provided a wealth of candida genomic data. we have developed the candida gene order browser (cgob), an online tool that aids comparative syntenic analyses of candida species. cgob incorporates all available candida clade genome sequences including two candida albicans isolates (sc5314 and wo-1) and 8 closely related species (candida dubliniensis, candida tropicalis, candida parap ... | 2010 | 20459735 |
| application of an oligonucleotide microarray-based nano-amplification technique for the detection of fungal pathogens. | the traditional techniques for diagnosis of invasive fungal infections in the clinical microbiology laboratory need improvement. these techniques are prone to delay results due to their time-consuming process, or result in misidentification of the fungus due to low sensitivity or low specificity. the aim of this study was to develop a method for the rapid detection and identification of fungal pathogens. | 2010 | 20618094 |
| chromosomal g + c content evolution in yeasts: systematic interspecies differences, and gc-poor troughs at centromeres. | the g + c content at synonymous codon positions (gc3s) in genes varies along chromosomes in most eukaryotes. in saccharomyces cerevisiae, regions of high gc3s are correlated with recombination hot spots, probably due to biased gene conversion. here we examined how gc3s differs among groups of related yeast species in the saccharomyces and candida clades. the chromosomal locations of gc3s peaks and troughs are conserved among four saccharomyces species, but we find that there have been highly con ... | 2010 | 20693156 |
| study of microbial contamination and dosing accuracy of oral dispensers. | the optimal administration of liquid medications requires accurate dose delivery. this is particularly important in the treatment of infants and children, as well as elderly people, who are more sensitive to dosage errors. previous studies revealed significant dosage inaccuracies with measuring spoons. oral syringes are therefore generally recommended instead. there is no data on the efficacy of standard cleaning techniques, and consequently on the degree of microbial contamination associated wi ... | 2010 | 20831530 |
| [mycological analysis of clinical materials of patients receiving total parenteral nutrition]. | the most frequent etiological factors of fungal infections in patients receiving total parenteral nutrition (tpn) belong to candida genus of the yeastlike fungi. in the tpn patients the several infectious complications can develop: venous catheter infection, catheter candidemia (fungemia), fungal endocarditis or fungal ophtalmitis. the capability of hydrolytic (proteolytic, lipolytic) enzymes secretion as well as biofilm formation on artificial surfaces are the most important factors of fungal s ... | 2010 | 20873489 |
| a two-step cloning-free pcr-based method for the deletion of genes in the opportunistic pathogenic yeast candida lusitaniae. | we describe a new cloning-free strategy to delete genes in the opportunistic pathogenic yeast candida lusitaniae. we first constructed two ura3δ strains in c. lusitaniae for their use in transformation experiments. one was deleted for the entire ura3 coding sequence; the other possessed a partial deletion within the coding region, which was used to determine the minimum amount of homology required for efficient homologous recombination by double crossing-over of a linear dna fragment restoring u ... | 2010 | 21204058 |
| combination of different molecular mechanisms leading to fluconazole resistance in a candida lusitaniae clinical isolate. | we report on the underlying molecular mechanisms likely responsible for the high-level fluconazole resistance in a candida lusitaniae clinical isolate. fluconazole resistance correlated with overexpression of erg11 and of several efflux pump genes, in particular, the orthologs of the candida albicans mdr1, pdr16, cdr1, cdr2, and yor1. | 2009 | 19070454 |
| nonsense and missense mutations in fcy2 and fcy1 genes are responsible for flucytosine resistance and flucytosine-fluconazole cross-resistance in clinical isolates of candida lusitaniae. | the aim of this work was to elucidate the molecular mechanisms of flucytosine (5fc) resistance and 5fc/fluconazole (flc) cross-resistance in 11 genetically and epidemiologically unrelated clinical isolates of candida lusitaniae. we first showed that the levels of transcription of the fcy2 gene encoding purine-cytosine permease (pcp) in the isolates were similar to that in the wild-type strain, 6936. nucleotide sequencing of the fcy2 alleles revealed that 5fc and 5fc/flc resistance could be corre ... | 2009 | 19414575 |
| phylogeny and evolution of the aspartyl protease family from clinically relevant candida species. | aspartyl proteases are a class of enzymes that include the yeast aspartyl proteases and secreted aspartyl protease (sap) superfamilies. several sap superfamily members have been demonstrated or suggested as virulence factors in opportunistic pathogens of the genus candida. candida albicans, candida tropicalis, candida dubliniensis and candida parapsilosis harbour 10, four, eight and three sap genes, respectively. in this work, genome mining and phylogenetic analyses revealed the presence of new ... | 2009 | 19547880 |
| candidaemia with uncommon candida species: predisposing factors, outcome, antifungal susceptibility, and implications for management. | the risk factors for and clinical features of bloodstream infection with uncommon candida spp. (species other than c. albicans, c. glabrata, c. parapsilosis, c. tropicals and c. krusei) are incompletely defined. to identify clinical variables associated with these species that might guide management, 57 cases of candidaemia resulting from uncommon candida spp. were analysed in comparison with 517 episodes of candida albicans candidaemia (2001-2004). infection with uncommon candida spp. (5.3% of ... | 2009 | 19614718 |
| candida albicans hyphal formation and virulence assessed using a caenorhabditis elegans infection model. | candida albicans colonizes the human gastrointestinal tract and can cause life-threatening systemic infection in susceptible hosts. we study here c. albicans virulence determinants using the nematode caenorhabditis elegans in a pathogenesis system that models candidiasis. the yeast form of c. albicans is ingested into the c. elegans digestive tract. in liquid media, the yeast cells then undergo morphological change to form hyphae, which results in aggressive tissue destruction and death of the n ... | 2009 | 19666778 |
| prosthetic valve endocarditis caused by candida lusitaniae, an uncommon pathogen: a case report. | candida lusitaniae was originally described as a human pathogen in 1979 and typically affects immunocompromised patients. | 2009 | 19830215 |
| determination of echinocandin mics for candida species in less than 8 hours: comparison of the rapid susceptibility assay with the clinical and laboratory standards institute's broth microdilution assay. | the echinocandins prevent fungal cell wall synthesis by inhibiting beta-1,3-glucan synthesis, a significant glucose-consuming process. previous studies suggested that echinocandin inhibitory activity is evident within 1 h of exposure. we hypothesized that a susceptibility assay based on glucose consumption may provide clinically useful mics rapidly. the rapid susceptibility assay (rsa), which provides mics in less than 8 h, was compared with the standard broth microdilution susceptibility assay ... | 2009 | 19846655 |
| molecular identification of yeasts associated with traditional egyptian dairy products. | this study aimed to examine the diversity and ecology of yeasts associated with traditional egyptian dairy products employing molecular techniques in yeast identification. a total of 120 samples of fresh and stored domiati cheese, kariesh cheese, and "matared" cream were collected from local markets and examined. forty yeast isolates were cultured from these samples and identified using the restriction-fragment length polymorphism (rflps) of 5.8s-its rdna region and sequencing of the domains d1 ... | 2009 | 19895478 |
| epidemiology and antifungal susceptibility of bloodstream candida isolates in quebec: report on 453 cases between 2003 and 2005. | between may 2003 and april 2005, a population-based surveillance of candida bloodstream infections was conducted in quebec. a total of 453 episodes of candidemia (464 yeast isolates) from 54 participating hospitals were studied. | 2008 | 19145263 |
| candida lusitaniae fungemia in cancer patients: risk factors for amphotericin b failure and outcome. | candida lusitaniae, a candida species frequently resistant to amphotericin b (amb), is a rare cause of candidemia. the clinical significance of this in vitro resistant phenotype, the risk factors for, and the clinical presentation of c. lusitaniae fungemia in comparison with those of candida albicans have not been completely characterized. we reviewed 13 consecutive cases of c. lusitaniae fungemia in cancer patients and compared them with 41 consecutive cases of c. albicans fungemia (1990-2004). ... | 2008 | 19180749 |
| comparison of disc diffusion assay with the clsi reference method (m27-a2) for testing in vitro posaconazole activity against common and uncommon yeasts. | to evaluate the suitability of disc diffusion (dd) assay for testing posaconazole activity and to corroborate its activity against recently isolated yeasts by the clsi reference microdilution m27-a2 method. | 2008 | 18033784 |
| candidadb: a multi-genome database for candida species and related saccharomycotina. | candidadb (http://genodb.pasteur.fr/candidadb) was established in 2002 to provide the first genomic database for the human fungal pathogen candida albicans. the availability of an increasing number of fully or partially completed genome sequences of related fungal species has opened the path for comparative genomics and prompted us to migrate candidadb into a multi-genome database. the new version of candidadb houses the latest versions of the genomes of c. albicans strains sc5314 and wo-1 along ... | 2008 | 18039716 |
| dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses. | an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ... | 2008 | 18160452 |
| candida lusitaniae septic arthritis: case report and review of the literature. | candida lusitaniae is an infrequently encountered candida species that has been associated with resistance to amphotericin b. we present a case of septic arthritis with c. lusitaniae and provide a brief review of the organism, especially in regard to current information about its pattern of resistance to antifungal agents. | 2008 | 18191360 |
| acute calculous cholecystitis caused by candida lusitaniae: an unusual causative organism in a patient without underlying malignancy. | candidiasis of the gallbladder is an uncommon cause of acute cholecystitis. candidal cholecystitis is seen especially in patients with malignancies. in the present case, we report that acute calculous cholecystitis was caused by candida lusitaniae in a 33-year-old patient without underlying malignancy. according to our review of the literature, this is the first report of acute cholecystitis caused by c. lusitaniae. | 2008 | 18362405 |
| contributions of the response regulators ssk1p and skn7p in the pseudohyphal development, stress adaptation, and drug sensitivity of the opportunistic yeast candida lusitaniae. | we recently characterized the histidine kinase receptor genes of candida lusitaniae. for the present study, we have further investigated the role of ssk1 and skn7, encoding response regulators. the results of functional analysis of mutants indicated that ssk1p is involved in osmotolerance and pseudohyphal development, whereas skn7p appears crucial for oxidative stress adaptation. | 2008 | 18408054 |
| identification of yeast and bacteria involved in the mezcal fermentation of agave salmiana. | aims: to identify the yeast and bacteria present in the mezcal fermentation from agave salmiana. methods and results: the restriction and sequence analysis of the amplified region, between 18s and 28s rdna and 16s rdna genes, were used for the identification of yeast and bacteria, respectively. eleven different micro-organisms were identified in the mezcal fermentation. three of them were the following yeast: clavispora lusitaniae, pichia fermentans and kluyveromyces marxianus. the bacteria foun ... | 2008 | 18489025 |
| evidence of recent interkingdom horizontal gene transfer between bacteria and candida parapsilosis. | to date very few incidences of interdomain gene transfer into fungi have been identified. here, we used the emerging genome sequences of candida albicans wo-1, candida tropicalis, candida parapsilosis, clavispora lusitaniae, pichia guilliermondii, and lodderomyces elongisporus to identify recent interdomain hgt events. we refer to these as ctg species because they translate the ctg codon as serine rather than leucine, and share a recent common ancestor. | 2008 | 18577206 |
| identification of yeasts isolated from commercial shell eggs stored at refrigerated temperatures. | yeasts and molds can grow on or in eggs, causing spoilage. washed and unwashed eggs (treatments) were collected aseptically on three separate days (replications) from a commercial processing facility and stored for 10 weeks at 4 degrees c. ten eggs from each treatment were sampled weekly (110 eggs per treatment per replication). yeasts and molds were enumerated from external shell rinses by plating onto acidified potato dextrose agar. yeast colonies were picked randomly and stored for subsequent ... | 2008 | 18592756 |
| debaryomyces hansenii (candida famata), a rare human fungal pathogen often misidentified as pichia guilliermondii (candida guilliermondii). | debaryomyces hansenii is a hemiascomycetous yeast commonly found in natural substrates and in various types of cheese. pichia guilliermondii is widely distributed in nature and is a common constituent of the normal human microflora. both species have been described in human infections but are extremely difficult to differentiate phenotypically. thus, frequent errors in identification occur. the 62 clinical and environmental isolates sent between 2000 and 2007 to the french national reference cen ... | 2008 | 18701668 |
| microbial diversity of traditional vietnamese alcohol fermentation starters (banh men) as determined by pcr-mediated dgge. | the diversity of fungi and bacteria associated with traditional vietnamese alcohol fermentation starters (banh men) was investigated by pcr-mediated dgge. from 52 starter samples, 13 species of fungi (including yeasts) and 23 species of bacteria were identified. the fungal composition of the starters was consistent with little variation among samples. it consisted of amylase producers (rhizopus oryzae, r. microsporus, absidia corymbifera, amylomyces sp., saccharomycopsis fibuligera), ethanol pro ... | 2008 | 18838186 |
| insight into the role of hog pathway components ssk2p, pbs2p, and hog1p in the opportunistic yeast candida lusitaniae. | in the present study, we have investigated the role of ssk2, pbs2, and hog1, encoding modules of the high-osmolarity-glycerol mitogen-activated protein kinase pathway in candida lusitaniae. functional analysis of mutants indicated that ssk2p, pbs2p, and hog1p are involved in osmotolerance, drug sensitivity, and heavy metal tolerance but not in oxidant stress adaptation. | 2008 | 18952902 |
| [in vitro amphotericin b susceptibility of korean bloodstream yeast isolates assessed by the clsi broth microdilution method, etest, and minimum fungicidal concentration test]. | although amphotericin b (amb) has a wide spectrum of activity that encompasses the majority of yeast isolates, there have been recent reports suggesting that some yeast isolates exhibit decreased susceptibility to amb. however, in vitro amb susceptibility of yeast species isolates from blood cultures in korea has not been fully surveyed. | 2008 | 18971615 |
| role of sho1p adaptor in the pseudohyphal development, drugs sensitivity, osmotolerance and oxidant stress adaptation in the opportunistic yeast candida lusitaniae. | in yeast, external signals such as high osmolarity or oxidant conditions activate the high osmolarity glycerol (hog) mitogen-activated protein kinase (mapk) cascade pathway, which consists of two upstream branches, i.e. sho1p and sln1p and common downstream elements, including the pbs2p mapk kinase and the hog1p mapk. we recently showed that the candida lusitaniae sln1 gene, potentially encoding a histidine kinase receptor, is crucial for oxidative stress adaptation when the fungus grows as budd ... | 2008 | 19061190 |
| differential involvement of histidine kinase receptors in pseudohyphal development, stress adaptation, and drug sensitivity of the opportunistic yeast candida lusitaniae. | fungal histidine kinase receptors (hkrs) sense and transduce many extracellular signals. we investigated the role of hkrs in morphogenetic transition, osmotolerance, oxidative stress response, and mating ability in the opportunistic yeast candida lusitaniae. we isolated three genes, sln1, nik1, and chk1, potentially encoding hkrs of classes vi, iii, and x, respectively. these genes were disrupted by a transformation system based upon the "ura3 blaster" strategy. functional analysis of disruptant ... | 2007 | 17660361 |
| candida lusitaniae arthritis in an intravenous drug user. | a case of arthritis of the right knee caused by candida lusitaniae in a 29-year-old intravenous drug abuser is described. the diagnosis was based on the isolation of c. lusitaniae from synovial fluid and was supported by the presence of c. lusitaniae-specific dna and high levels of (1-3)-beta-d-glucan (122 pg ml-1) in the same specimen. while the isolate was susceptible to amphotericin b and fluconazole in vitro, treatment with amphotericin b was not very effective. the patient achieved complete ... | 2007 | 17714366 |
| dna microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients. | the increasing incidence of invasive fungal infections (ifi) in immunocompromised patients emphasizes the need to improve diagnostic tools. we established a dna microarray to detect and identify dna from 14 fungal pathogens (aspergillus fumigatus, aspergillus flavus, aspergillus terreus, candida albicans, candida dubliniensis, candida glabrata, candida lusitaniae, candida tropicalis, fusarium oxysporum, fusarium solani, mucor racemosus, rhizopus microsporus, scedosporium prolificans, and trichos ... | 2007 | 17715373 |
| changing pattern of candidaemia 2001-2006 and use of antifungal therapy at the university hospital of vienna, austria. | a retrospective survey of candidaemia between 2001 and 2006 was performed at the university hospital of vienna, a 2200-bed centre with large organ transplantation and haematology-oncology units. the incidence rate of candida spp. in blood cultures increased from 0.27 cases/1000 admissions in 2001 to 0.77 cases/1000 admissions in 2006 (p <0.005). the incidence of candidaemia caused by candida albicans and by non-albicans candida spp. both increased during this period; although there was a trend t ... | 2007 | 17725647 |
| the pan-ac assay: a single-reaction real-time pcr test for quantitative detection of a broad range of aspergillus and candida species. | in view of the growing incidence and the high mortality of invasive aspergillosis and candidiasis, adequate diagnostic techniques permitting timely onset of treatment are of paramount importance. more than 90 % of all invasive fungal infections in immunocompromised individuals can be attributed to candida and aspergillus species. to date, standardized techniques permitting rapid, sensitive and, no less importantly, economic screening for the clinically most relevant fungi are lacking. in the pre ... | 2007 | 17761478 |
| effect of lemon extract on foodborne microorganisms. | a quantitative investigation was conducted on the antimicrobial effect of lemon extract against some food spoilage microorganisms: yeasts, bacillus species, and lactic acid bacteria. growth kinetics and dose-response profiles were determined from experimental data obtained with a suitable macrodilution methodology based on a turbidimetric technique. growth and no-growth status of microbial suspensions were expressed in terms of noninhibitory concentration (nic) and mic. lemon extract was effecti ... | 2007 | 17803147 |
| correlation of posaconazole minimum fungicidal concentration and time kill test against nine candida species. | we evaluated the in vitro activity of posaconazole against nine candida species using minimum fungicidal concentration (mfc) measurements and time-kill methods. | 2007 | 17875607 |
| development of primers for detecting dominant yeasts in smear-ripened cheeses. | pcr primers were developed for the specific detection of clavispora lusitaniae, debaryomyces hansenii var hansenii, geotrichum candidum, kluyveromyces lactis and k. marxianus and yarrowia lipolytica, yeast species commonly found on the surface of smear cheese. forty eight representative strains frequently found in smear cheeses or taxonomically related to the target yeasts were used as templates, to validate the designed primers. the specific and selective detection of these yeasts was effective ... | 2007 | 17291384 |
| stability of the biodiversity of the surface consortia of gubbeen, a red-smear cheese. | a total of 1,052 bacteria and 828 yeasts were isolated from the surface flora of 6 batches of gubbeen cheese made in 1996-1997 and 2002-2003. stability of the microflora was evaluated over time and also during ripening at 4, 10, and 16 d (batches 4, 5, and 6) or at 4, 16, 23, and 37 d (batches 1, 2, and 3). bacteria were identified using pulsed-field gel electrophoresis, repetitive extragenic palindromic-pcr, and 16s rrna gene sequencing, and yeasts were identified by fourier transform infrared ... | 2007 | 17430918 |
| differentiation of candida albicans from non-albicans yeast directly from blood cultures by gram stain morphology. | clusters of pseudohyphae are commonly seen on gram stain of blood cultures from patients with candida albicans fungemia. whether this morphologic feature is useful for differentiating c. albicans from other yeasts has not previously been systematically evaluated. yeast morphology on gram stain of blood cultures from consecutive patients with fungemia detected by the bactec automated blood culture system was prospectively assessed and correlated with the final culture-based yeast identification. ... | 2007 | 17447090 |
| surface charge influences enterococcal prevalence in mixed-species biofilms. | to study the influence of 15 microbial isolates on the prevalence of charge-heterogeneous and charge-homogeneous enterococcus faecalis strains, all isolated from biliary stents, in mixed-species biofilms. | 2007 | 17448160 |
| species identification and strain differentiation of clinical candida isolates using the diversilab system of automated repetitive sequence-based pcr. | the diversilab system, which uses repetitive sequence-based pcr (rep-pcr) to genotype micro-organisms, was evaluated as a molecular typing tool for members of the genus candida. initially, 41 clinical candida spp. (7 candida krusei, 10 candida parapsilosis, 7 candida albicans, 10 candida tropicalis and 7 candida glabrata), previously identified at the species level by morphological and biochemical analysis, were analysed with the diversilab system. species identification was confirmed by dna seq ... | 2007 | 17510263 |
| ce separation of proteins and yeasts dynamically modified by peg pyrenebutanoate with fluorescence detection. | the optimized protocols of the bioanalytes separation, proteins and yeasts, dynamically modified by the nonionogenic tenside peg pyrenebutanoate, were applied in cze and cief with the acidic gradient in ph range 2-5.5, both with fluorescence detection. peg pyrenebutanoate was used as a buffer additive for a dynamic modification of proteins and/or yeast samples. the narrow peaks of modified analytes were detected. the values of the pi's of the labeled proteins were calculated using new fluorescen ... | 2007 | 17557360 |
| [dacryocystitis caused by candida lusitaniae]. | case report: we report the case of a 60-year-old woman with recurrent dacryocystitis of the right lacrimal sac. she did not recover after medical treatment, nor after dacryocystorhinostomy with canalicular intubation. she also had dacryocystitis on the left side. the material that was obtained during surgery was grown in a culture medium and candida lusitaniae was identified as the responsible organism. all symptoms disappeared after topical treatment with amphotericin b and dacryocystorhinostom ... | 2007 | 17573647 |
| comparison of a randomly amplified polymorphic dna (rapd) analysis and atb id 32c system for identification of clinical isolates of different candida species. | the objective of this work was to compare the usefulness of a randomly amplified polymorphic dna (rapd) assay to that of the atb id32c kit (biomérieux, france) for identification of different species of candida isolated from clinical specimens. the rapd-pcr patterns obtained with ope-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed reproduction of the band patterns. rapd with the ope-18 primer is a very specific and sensitive method ... | 2007 | 17604435 |
| molecular mechanism of flucytosine resistance in candida lusitaniae: contribution of the fcy2, fcy1, and fur1 genes to 5-fluorouracil and fluconazole cross-resistance. | inactivation of the fcy2 (cytosine permease), fcy1 (cytosine deaminase), and fur1 (uracil phosphoribosyltransferase) genes in candida lusitaniae produced two patterns of resistance to flucytosine. mutant fur1 demonstrated resistance to 5-fluorouracil, whereas mutants fcy1 and fcy2 demonstrated fluconazole resistance in the presence of subinhibitory flucytosine concentrations. | 2007 | 17060521 |
| fluconazole susceptibility testing of candida species by flow cytometry. | currently, antifungal drug susceptibility testing is labor-intensive, limited by delays in obtaining results and high costs. the purpose of this study was to determine the usefulness of flow cytometry (fcm) antifungal drug susceptibility testing as a routine laboratory procedure. | 2007 | 17084902 |
| one year prospective survey of candida bloodstream infections in scotland. | a 12 month survey of candidaemia in scotland, uk, in which every scottish hospital laboratory submitted all blood isolates of yeasts for identification, strain typing and susceptibility testing, provided 300 isolates from 242 patients, generating incidence data of 4.8 cases per 100,000 population per year and 5.9 cases per 100,000 acute occupied bed days; 27.9 % of cases occurred in intensive care units. more than half the patients with candidaemia had an underlying disease involving the abdomen ... | 2007 | 17644714 |
| [the fungicidal activity and paradoxical effect of caspofungin against yeast. influence of culture medium and incubation time]. | the effect of culture medium and incubation time on the fungicidal activity of caspofungin and on the frequency of paradoxical effect has been studied in 144 blood culture isolates (42, candida tropicalis, 32 candida glabrata, 21 candida krusei, 20 candida parapsilosis, 11 candida guilliermondii, 4 candida famata, 3 candida lusitaniae, 3 blastoschizomyces capitatus, 2 saccharomyces cerevisiae, 2 yarrowia lipolytica, 1 candida inconspicua, 1 candida lambica, 1 candida sake y 1 pichia ohmeri). the ... | 2007 | 18563217 |
| a fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis. | to date, most fungal phylogenies have been derived from single gene comparisons, or from concatenated alignments of a small number of genes. the increase in fungal genome sequencing presents an opportunity to reconstruct evolutionary events using entire genomes. as a tool for future comparative, phylogenomic and phylogenetic studies, we used both supertrees and concatenated alignments to infer relationships between 42 species of fungi for which complete genome sequences are available. | 2006 | 17121679 |
| global surveillance of in vitro activity of micafungin against candida: a comparison with caspofungin by clsi-recommended methods. | micafungin is an echinocandin antifungal agent that has recently been approved for the prevention of invasive fungal infection and the treatment of esophageal candidiasis. prospective sentinel surveillance for the emergence of in vitro resistance to micafungin among invasive candida sp. isolates is indicated. we determined the in vitro activity of micafungin against 2,656 invasive (bloodstream or sterile site) unique patient isolates of candida spp. collected from 60 medical centers worldwide in ... | 2006 | 17021079 |
| [restropective investigation about the treatment of candidaemia in a french university hospital in 2004]. | this study aimed to retrospectively evaluate the adequate use of antifungal drugs in the treatment of the candidaemia. a collection of clinical, biological and therapeutic data was carried out for the patients who had a positive blood culture for candida species during the year 2004. the antifungal therapy was compared to the guidelines of the french conference of consensus named "prise en charge des candidoses et aspergilloses invasives de l'adulte". the degree of conformity was classified as f ... | 2006 | 17045421 |
| the discovery of moriniafungin, a novel sordarin derivative produced by morinia pestalozzioides. | a novel sordarin derivative, moriniafungin (1), containing a 2-hydroxysebacic acid residue linked to c-3' of the sordarose residue of sordarin through a 1,3-dioxolan-4-one ring was isolated from the fungus morinia pestalozzioides. isolation of moriniafungin employed a highly specific bioassay consisting of a panel of saccharomyces cerevisiae strains containing chimeric eef2 for candida glabrata, candida krusei, candida lusitaniae, crytpococcus neoformans, and aspergillus fumigatus as well as wil ... | 2006 | 16183294 |
| in vitro pharmacodynamics of rapid versus continuous infusion of amphotericin b deoxycholate against candida species in the presence of human serum albumin. | recent open label studies have suggested that dosing amphotericin b (amb) by continuous infusion (ci) may reduce drug-associated infusion reactions and nephrotoxicity. in vitro and in vivo pharmacodynamic (pd) data, however, do not consistently support the concept of ci dosing based on the concentration-dependent activity of this agent and in vitro studies with amb rarely account for the drug's high degree of protein binding. therefore, we compared the pd activity of simulated continuous versus ... | 2006 | 16387749 |
| intrinsic in vitro susceptibility of primary clinical isolates of aspergillus fumigatus, aspergillus terreus, aspergillus nidulans, candida albicans and candida lusitaniae against amphotericin b. | a total of 60 clinical fungal isolates from patients without prior amphotericin b treatment and three control strains were evaluated for their intrinsic susceptibility to amphotericin b (amb) using microdilution, etest and disc diffusion assays, on three media each, roswell park memorial institute (rpmi) 1640, antibiotic medium 3 (am3) and high resolution medium. the fungal strains included isolates of aspergillus fumigatus (n = 10), aspergillus terreus (n = 12), aspergillus nidulans (n = 9), ca ... | 2006 | 16466441 |
| activity of novel non-amphipathic cationic antimicrobial peptides against candida species. | candida species are problematic opportunistic pathogens in the hospital setting, where they are frequently associated with opportunistic infections of indwelling medical devices. there are only a few effective classes of antifungal agents currently available, and some species, such as candida lusitaniae, candida glabrata and candida krusei, are intrinsically resistant to some of these drugs, further reducing existing therapeutic options. we have recently developed synthetic, non-amphipathic cati ... | 2006 | 16524895 |
| polymicrobial keratomycosis in a three-year-old child. | to describe a previously unreported case of polymicrobial mycotic keratitis caused by an association between candida lusitaniae, c. parapsilosis, and geotrichum candidum. | 2006 | 16597544 |
| phenotypic switching in candida lusitaniae on copper sulfate indicator agar: association with amphotericin b resistance and filamentation. | candida lusitaniae is an opportunistic yeast pathogen that has the ability to develop resistance to amphotericin b (amb). the mechanism(s) for this resistance is not well understood, although there are data supporting mutations in sterol pathways and other data supporting phenotypic switching (ps). the goal of this study was to determine whether c. lusitaniae has a ps system and to characterize any phenotypes, including any changes in amb mics. when 10(4) cfu of an amb-resistant (mic of 16 to 32 ... | 2006 | 16597887 |
| changing incidence of candida bloodstream infections among nicu patients in the united states: 1995-2004. | recent reports suggest that candidemia caused by fluconazole-resistant strains is increasing in certain adult populations. we evaluated the annual incidence of neonatal candidemia and the frequency of disease caused by different species of candida among neonates in the united states. | 2006 | 16651324 |
| novel lysine biosynthetic gene sequences (lys1 and lys5) used as pcr targets for the detection of the pathogenic candida yeast. | we report here a sensitive and specific polymerase chain reaction (pcr) detection assay for the pathogenic candida yeast based on the novel lys1 [encoding saccharopine dehydrogenase (sdh)] and lys5 [encoding phosphopantetheinyl transferase (pptase)] gene sequences of the fungal unique lysine biosynthetic pathway. both lys1 and lys5 dna-specific pcr primers sg1, sg2 and sg3, sg4, respectively, amplified predicted 483 and 648-bp fragments from candida albicans genomic dna but not from other select ... | 2006 | 16733732 |
| specificity of the monoclonal antibody 3h8 in the immunohistochemical identification of candida species. | candida albicans has been shown to be involved in the pathogenesis of adult periodontitis (ap). the diagnosis of candida-associated ap depends largely on the identification of yeast and pseudomycelial forms in gingival tissue samples by using periodic acid-schiff and gomori methenamine silver stains. however, these stains are non-specific and also reveal confusing artifacts seemingly rather difficult to distinguish from yeasts. with the recent development and availability of monoclonal antibodie ... | 2006 | 16792731 |
| candida lusitaniae and chronic granulomatous disease. | 2006 | 16874186 | |
| development of a dna microarray for detection and identification of fungal pathogens involved in invasive mycoses. | invasive fungal infections have emerged as a major cause of morbidity and mortality in immunocompromised patients. conventional identification of pathogenic fungi in clinical microbiology laboratories is time-consuming and, therefore, often imperfect for the early initiation of an adequate antifungal therapy. we developed a diagnostic microarray for the rapid and simultaneous identification of the 12 most common pathogenic candida and aspergillus species. oligonucleotide probes were designed by ... | 2005 | 16207946 |
| yeast community survey in the tagus estuary. | the yeast community in the waters of the tagus estuary, portugal, was followed for over a year in order to assess its dynamics. yeast occurrence and incidence were measured and this information was related to relevant environmental data. yeast occurrence did not seem to depend upon tides, but river discharge had a dramatic impact both on the density and diversity of the community. the occurrence of some yeasts was partially correlated with faecal pollution indicators. yeast isolates were charact ... | 2005 | 16329949 |
| antimicrobial activity of flavonoids from pelargonium radula (cav.) l'hérit. | flavonoids from pelargonium radula (cav.) l'hérit were purified by column chromatography. two fractions were obtained: f1 (main flavonoid isoquercitrin) and f2 (main flavonoid rutin). in vitro antimicrobial activity of f1 and f2 were tested against eleven species of bacteria and eleven species of fungi. both fractions demonstrated strong inhibitory activity against staphylococcus aureus, proteus rettgeri, candida tropicalis and microsporum gypseum. staphylococcus sp. (coagulase-negative) and can ... | 2005 | 16375833 |
| antifungal susceptibilities of clinical isolates of candida species, cryptococcus neoformans, and aspergillus species from taiwan: surveillance of multicenter antimicrobial resistance in taiwan program data from 2003. | the susceptibilities of nonduplicate isolates to six antifungal agents were determined for 391 blood isolates of seven candida species, 70 clinical isolates (from blood or cerebrospinal fluid) of cryptococcus neoformans, and 96 clinical isolates of four aspergillus species, which were collected in seven different hospitals in taiwan (as part of the 2003 program of the study group surveillance of multicenter antimicrobial resistance in taiwan). all isolates of candida species other than c. glabra ... | 2005 | 15673726 |
| differentiation between atypical isolates of candida lusitaniae and candida pulcherrima by determination of mating type. | we report on five clinical isolates routinely identified as candida lusitaniae that the id 32c system was unable to discriminate from the closely related species candida pulcherrima. when additional tests did not allow accurate identification, the less usual mating type test identified all of them as clavispora lusitaniae. mating type testing appears to be a valuable tool for assessing the true incidence of this emerging non-albicans candida species. | 2005 | 15750124 |
| chromagar candida medium for direct susceptibility testing of yeast from blood cultures. | an evaluation was performed on 95 blood cultures positive for candida spp. to determine the correlation of direct susceptibility testing of fluconazole versus both standardized disk diffusion and mic methods. for direct testing, an aliquot taken from bd bactec plus and/or bd bactec lytic/10 bottles (becton dickinson [bd], sparks, md) positive by gram stain for yeast was subcultured to chromagar candida (bd), and a 25-microg fluconazole disk (bd) was placed on the plate. the area of growth inhibi ... | 2005 | 15814992 |
| antifungal susceptibilities of candida species causing vulvovaginitis and epidemiology of recurrent cases. | there are limited data regarding the antifungal susceptibility of yeast causing vulvovaginal candidiasis, since cultures are rarely performed. susceptibility testing was performed on vaginal yeast isolates collected from january 1998 to march 2001 from 429 patients with suspected vulvovaginal candidiasis. the charts of 84 patients with multiple positive cultures were reviewed. the 593 yeast isolates were candida albicans (n = 420), candida glabrata (n = 112), candida parapsilosis (n = 30), candi ... | 2005 | 15872235 |
| antifungal susceptibility of candida spp. isolated from pediatric patients: a survey of 4 children's hospitals. | candida species are the most common cause of fungal infections in hospitalized patients. recent studies have reported a relative reduction in the rates of infection caused by candida albicans and a shift toward non-albicans candida spp. data on the distribution and susceptibility of candida spp. from children's hospitals are limited. clinical isolates of candida were collected from 4 us children's hospitals in 2003. broth dilution mics for amphotericin b, fluconazole, voriconazole, caspofungin, ... | 2005 | 15922535 |
| inactivation of the fcy2 gene encoding purine-cytosine permease promotes cross-resistance to flucytosine and fluconazole in candida lusitaniae. | in a previous work, we described the possible relationship between a defect of purine-cytosine permease and the acquisition of a cross-resistance to the antifungal combination flucytosine (5fc) and fluconazole (flc) in candida lusitaniae (t. noël, f. françois, p. paumard, c. chastin, d. brethes, and j. villard, antimicrob. agents chemother. 47:1275-1284, 2003). using degenerate pcr and chromosome walking, we cloned two fcy2-like genes in c. lusitaniae. northern blot analysis revealed that only o ... | 2005 | 16048910 |
| [fungal endocarditis found at onset of lower limb acute aortic occlusion; report of a case]. | fungal endocarditis caused by candida species is associated with high morbidity and mortality. a combination of surgical resection and antifungal drug therapy is the golden standard for treatment. we reported a case of fungal endocarditis due to candida lusitaniae found at onset of lower limb acute aortic occlusion cured by emergency operation. this case suggests that candida endocariditis can be managed medically with antifungal drug therapy in life time. | 2005 | 16104572 |