Publications

genomic sequence of the pathogenic and allergenic filamentous fungus aspergillus fumigatus.	aspergillus fumigatus is exceptional among microorganisms in being both a primary and opportunistic pathogen as well as a major allergen. its conidia production is prolific, and so human respiratory tract exposure is almost constant. a. fumigatus is isolated from human habitats and vegetable compost heaps. in immunocompromised individuals, the incidence of invasive infection can be as high as 50% and the mortality rate is often about 50% (ref. 2). the interaction of a. fumigatus and other airbor ...	2005	16372009
purification and characterization of a low molecular weight endoxylanase from solid-state cultures of alkali-tolerant aspergillus fischeri.	a low molecular weight, alkaline-stable endoxylanase (xylb) was purified to homogeneity from solid-state culture of aspergillus fischeri fxn1. xylb had a molecular mass of 13 kda which is the lowest of reported xylanases. optimal activity was at ph 6 and 55 degrees c. xylb was stable from ph 4.5 to 10 and up to 60 degrees c. it was non-glycosylated. the apparent k(m) and v(max) values of xylb on birch wood xylan were 0.53 mg ml(-1) and 0.2 mmol min-1 mg-1, respectively. the activity of xylb was ...	2004	15483388
target selection in designing pasteurization processes for shelf-stable high-acid fruit products.	this study is focused on the search for targets and criteria for the design of pasteurization processes for high-acid shelf-stable fruit products, such as juices, nectars, pastes, purees, concentrates, jams, jellies, etc. first, an overview of pasteurization is presented and then, frequently used targets for pasteurization processes are reviewed enzymes naturally present in fruits, in decreasing order of heat resistance, were pectinesterase, peroxidase, and polyphenoloxidase, and they may be use ...	2004	15540648
detection of viable fungal spores contaminant on documents and rapid control of the effectiveness of an ethylene oxide disinfection using atp assay.	filamentous fungi are able to damage and even destroy archival and library materials. nowadays the conventional method for detecting such micro-organisms is to put them in cultures but such methods are laborious and time-consuming. atp methodology has been widely applied in other domains and its success on bacteria and yeast has been demonstrated. several commercial reagent kits are available but they did not give satisfactory results on spores mould. we have elaborated new extraction strategies ...	2003	12687632
growth modelling of heat-resistant fungi: the effect of water activity.	growth of three strains of heat-resistant fungi, byssochlamys fulva, neosartorya fischeri and talaromyces avellaneus, was studied at 25 degrees c at eight or nine water activities (a(w)) adjusted with sucrose between 0.995 and 0.85. the flexible growth model of baranyi describing the change in colony diameter (mm) with respect to time was first fitted to measured growth data and from the fitted curves the maximum colony growth rates were estimated. these values were fitted with respect to a(w) t ...	2001	11205942
identification of the configuration of neosartorin by long-range nuclear overhauser effect measurements.	the relative configuration of the two xanthene units of neosartorin, a new ergochrome biosynthesised by the soil mould neosartorya fischeri, was determined using a 1d double-pulsed field gradient spin-echo noesy experiment. it was found that both units have the same relative stereochemistry. long-range nonbonding interactions between the substituents of different xanthene units stabilise the nonplanar configuration of the two aromatic rings a and a' connecting both monomer units of neosartorin.	2001	11579447
genetic variability within the aspergillus viridinutans species.	the intraspecific variability of the aspergillus viridinutans species was examined using various techniques including morphological examinations, carbon-source-utilization tests, restriction-enzyme analysis of the mitochondrial and nuclear dna, and sequence analysis of part of the beta-tubulin gene. although the genetic distances between the isolates were higher than between, e.g., a. fumigatus and neosartorya fischeri, most a. viridinutans isolates, together with n. aureola and n. udagawae stra ...	2000	11347269
mitochondrial cytochrome b gene analysis of aspergillus fumigatus and related species.	nucleotide sequences of 426 bp from the mitochondrial (mt) cytochrome b genes of six anamorph species and two species of neosartorya teleomophs of aspergillus section fumigati were determined. these sequences were used to build nucleotide- and amino acid-based trees for phylogenetic analysis. thirteen strains of a. fumigatus including 10 clinical isolates of a. fumigatus, 1 type culture of a. fumigatus var. fumigatus, 1 type culture of a. fumigatus var. ellipticus, and 1 strain of a. fumigatus v ...	2000	10747106
thermal death rate of ascospores of neosartorya fischeri atcc 200957 in the presence of organic acids and preservatives in fruit juices.	heat-resistant molds, including neosartorya fischeri, are known to spoil thermally processed fruit products. the control measures required for such problems must not cause an appreciable loss of the organoleptic qualities of the final products. in the present study we determined the thermal death rates of ascospores of n. fischeri atcc 200957 in fruit juices containing organic acids and preservatives. the ascospores were able to survive for more than 6 h of heating at 75 degrees c, 5 h at 80 deg ...	1998	9798155
simultaneous aspergillus fischeri and herpes simplex pneumonia in a patient with multiple myeloma.	a patient with light chain myeloma complicated by simultaneous herpes simplex and aspergillus fischeri pneumonia is presented. microscopic examination of her bronchoalveolar specimen showed bronchial cells with cytopathic effects and numerous cleistotheca, the sexual reproductive structures of aspergillus. culture was positive for herpes simplex virus and aspergillus fischeri. the initial partial response to amphotericin b followed by complete clinical response with addition of intravenous acycl ...	1998	9730310
toxinogenicity of heat-resistant fungi detected by a bio-assay.	the ciliostatic activity of exo- and endometabolites of 125 (100%) heat-resistant fungal isolates was evaluated in vitro by a bioassay on tracheal tissue cultures of 1 day old chicks. for 11 (9%) of the isolates investigated chloroform-extractable secondary metabolites were detected in the medium. for 29 (23%) they were detected in mycelium and spores and for 25 (20%) they were detected in the medium, mycelium and spores at the same time. especially dichotomomyces cejpii and eupenicillium baarne ...	1997	9217113
evaluation of growth of neosartorya spp. on some nigerian fruit products.	growth of neosartorya fischeri, n. fischeri var. spinosa and n. quadricincta on inoculated fruit juice agars (fjas) consisting of 1% agar and juices from mango (mja), orange (oja) and pineapple (pja) containing various levels of added sucrose was studied in terms of the average number of colonies produced by their ascospores, time (days) required before colonies were formed and developed to confluent mycelial mat as well as the level of reproductive spores produced. the means of the number of co ...	1997	9650724
shelf life study of some nigerian fruit juices inoculated with ascospores of neosartorya spp.	mango, orange and pineapple juices containing various concentrations of sucrose were dispensed in 100 ml volumes into 100 ml conical flasks with stoppers. each juice in a flask was inoculated with ascospores of one local isolate of either neosartorya fischeri, n. fischeri var. spinosa or n. quadricincta to a final concentration of 120-140 ascospores/ml of juice, and then pasteurized at 80 degrees c for 30 min. a set of 36 inoculated flasks with 12 controls was stored at 4-5 degrees c and two set ...	1997	9477427
morphological characteristics of aspergillus fumigatus strains isolated from patient samples.	the aspergillus fumigatus group splits into the anamorphic a. fumigatus series and the teleomorphic neosartorya fischeri series. the species a. fumigatus fresenius is the most common species within the a. fumigatus series. forty-seven clinical isolates out of the a. fumigatus group were obtained from patients with diseases such as localized and invasive aspergilloses or allergic disorders due to aspergillus and were mainly isolated from respiratory secretions. isolates were studied concerning ma ...	1997	9470420
neosartorya fischeri: an invasive fungal pathogen in an allogeneic bone marrow transplant patient.	invasive fungal infections are a complication of allogeneic bmt. we report the first case of a neosartorya fischeri fungal infection in a patient following allogeneic bmt. neosartorya fischeri is related to aspergillus fumigatus, but it is a distinct fungal species. despite granulocytic engraftment and aggressive anti-fungal therapy with amphotericin b, the patient died of overwhelming fungal infection on day 60 post-bmt. neosartorya fischeri is a pathogen that grows slowly in culture which can ...	1997	9156256
purification and characterization of xylanase from alkali-tolerant aspergillus fischeri fxn1.	alkali-tolerant aspergillus fischeri fxn1 produced two extracellular xylanases. the major xylanase (m(r) 31,000) was purified to electrophoretic homogeneity by ammonium sulfate precipitation, anion exchange chromatography and preparatory page. xylose was the major hydrolysis product from oat spelt and birch wood xylans. it was completely free of cellulolytic activities. the optimum ph and temperature were 6.0 and 60 degrees c, respectively. ph stability ranged from 5 to 9.5 and the t1/2 at 50 de ...	1996	8978101
production of type ii ribotoxins by aspergillus species and related fungi in taiwan.	a molecular investigation was conducted on the production of type ii ribotoxin of the species aspergillus and related fungi in taiwan. species that carried ribotoxin were confirmed by (1) cross-reactivity to anti-alpha-sarcin serum; (2) southern dot hybridization; (3) pcr amplification of genomic dna with specific primers; and (4) analysis of ribotoxic activity. five new strains, a. clavatus, a. oryzae var. effusus, a. ostianus, a. tamarii, and neosartorya fischeri var. spinosa, were identified ...	1995	7778122
molecular characterization of the food-borne fungus neosartorya fischeri (malloch and cain).	the food-borne fungus neosartorya fischeri, which is phenotypically related to the human opportunistic pathogen aspergillus fumigatus, causes spoilage of heat-processed fruit products. genomic methods were used to type n. fischeri strains and identify the genomic relationship between a. fumigatus and n. fischeri and between the different varieties of n. fischeri. ecori restriction fragment length polymorphism (rflp) patterns obtained after ethidium bromide staining could differentiate most of n. ...	1995	7747958
heat resistant fungi isolated from soil.	the thermal death time values (tdt) were estimated for dichotomomyces cejpii, gilmaniella humicola, talaromyces avellaneus and talaromyces bacillisporus isolated from soil. tdt values were compared with the tdt values of the known heat-resistant species, byssochlamys nivea, neosartorya fischeri and talaromyces flavus. all species studied showed considerable heat resistance. the most resistant species talaromyces avellaneus (172 cfu, initial concentration) of the isolates with unknown heat resist ...	1994	7986681
resistance of neosartorya fischeri to wet and dry heat.	dry heat resistance parameters for neosartorya fischeri ascospores were obtained at 90 degrees c and 95 degrees c under 30%, 40%, 50%, 60%, and 75% relative humidity (rh) conditions. the dry heat treated spores were exposed to saturated water vapor (for 20-24 h at 4 degrees c) prior to recovery in buffer held at two temperatures (0 degrees c and 80 degrees c). approximately the same level of recovery was obtained at the two buffer temperatures except at the shortest heating times for the heat tr ...	1994	8004412
heat-resistant fungi of importance to the food and beverage industry.	spoilage of pasteurized and canned fruit and fruit products caused by heat-resistant molds have been reported repeatedly in recent years. species most commonly implicated in fruit and fruit product disintegration are byssochlamys fulva, byssochlamys nivea, neosartorya fischeri, talaromyces flavus, and eupenicillium brefeldianum. these organisms are saprophytic rather than parasitic and usually contaminate fruits on or near the ground. they can survive heat treatments used for fruit processing an ...	1994	7857517
heat resistance of fungi from soil.	the survival of fungi from soil samples has been investigated after exposure to temperatures of 60, 70, 80 and 90 degrees c in sabouraud agar. the least resistant fungi surviving 60 degrees c for 60 min were the aspergillus (a.) niger group, chaetomium spp, penicillium spp, and scytalidium lignicola. none of these survived 70 degrees c for 10 min. the next group surviving 70 degrees c for 60 min included the a. glaucus group, byssochlamys nivea, dichotomomyces cejpii, gelasinospora spp, rhizocto ...	1993	8217516
survey of mycoflora and mycotoxins in egyptian soybean seeds.	after four months in commercial storage, 100 soybean samples from different places of egyptian governorates were assayed for filamentous fungal growth at two incubation temperatures (28 and 45 degrees c). 73 species and 8 varieties belonging to 32 genera were isolated by the dilution plate method. at 28 degrees c, the common species were aspergillus flavus, a. fumigatus, a. niger and a. alutaceus, followed by a. terreus, penicillium chrysogenum, p. citrinum, mucor hiemalis, m. racemosus, emerice ...	1993	8271157
fiscalins: new substance p inhibitors produced by the fungus neosartorya fischeri. taxonomy, fermentation, structures, and biological properties.	three new compounds, named fiscalins a, b, and c, were found in culture broth produced by a neosartorya fischeri. these compounds inhibit the binding of radiolabeled substance p ligand to the human neurokinin (nk-1) receptor, with ki values of 57, 174, and 68 microm, respectively. detailed spectroscopic and amino acid analyses led to the elucidation of structures for the three fiscalins. the structures contain an indolyl moiety linked to an athranilic acid derived tricyclic system. the absolute ...	1993	7684734
factors affecting recovery of neosartorya fischeri ascospores after exposure to dry heat.	recovery of neosartorya fischeri ascospores subjected to a dry heat treatment (dht) at 95 degrees c, 50% relative humidity (rh) for 60 minutes increased exponentially as the initial temperature of the recovery buffer increased. different diluents were evaluated and the same recovery pattern was obtained when water or dilute buffers were used to recover the dht spores. however, when glycerol was added to the buffer, the number of spores recovered in solutions held in ice water increased with incr ...	1993	8120735
5-n-acetylardeemin, a novel heterocyclic compound which reverses multiple drug resistance in tumor cells. ii. isolation and elucidation of the structure of 5-n-acetylardeemin and two congeners.	a family of novel compounds has been detected and isolated following an assay for the attenuation of multiple drug resistance in tumor cells from the fermentation broth and mycelia of a strain of aspergillus fischeri which we have designated var. brasiliensis. the structures of three components were determined employing 1-d and 2-d homonuclear and heteronuclear nmr spectroscopy and mass spectrometry. the structure of 5-n-acetylardeemin was confirmed by single crystal x-ray diffraction. these com ...	1993	8478256
5-n-acetylardeemin, a novel heterocyclic compound which reverses multiple drug resistance in tumor cells. i. taxonomy and fermentation of the producing organism and biological activity.	the ardeemins are a new family of secondary metabolites produced by submerged fermentation of a fungus which was isolated from a soil sample collected in brazil. based on taxonomic studies, the producing culture was identified as aspergillus fischeri var. brasiliensis strain ab 1826m-35. 5-n-acetylardeemin potentiated the cytotoxicity of the anticancer agent vinblastine in multidrug resistant human tumor cells.	1993	8478255
structure of fischerin, a new toxic metabolite from an ascomycete, neosartorya fischeri var. fischeri.	a new toxic metabolite named fischerin [1] from an ascomycete. neosartorya fischeri var. fischeri, which caused lethal peritonitis in mice, was deduced to have a structure including a 1,4-dihydroxy-3,5-disubstituted-2(1h)-pyridone moiety by chemical and spectral data.	1993	8229011
heat-resistant fungi in the soil.	the occurrence of heat-resistant fungi has been demonstrated in samples of soil from the slovak republic. the heat-resistant species isolated were byssochlamys nivea, dichotomomyces cejpii, eupenicillium baarnense, neosartorya fischeri, talaromyces avellaneus, tal. bacillisporus, tal. emersonii, tal. flavus, tal. trachyspermus, tal. wortmanii, botryotrichum piluliferum, gilmaniella humicola and nodulisporium sp. some of them were isolated for the first time from czechoslovakian soil. for the var ...	1992	1445767
graft-related endocarditis caused by neosartorya fischeri var. spinosa.	the first case of endocarditis caused by neosartorya fischeri var. spinosa is reported. the patient was a child who received a calf pericardium graft after removal of a previously inserted dacron graft associated with deterioration of adjacent tissue. copious vegetations removed from the heart were found to be composed of septate hyaline fungal filaments. the fungus was recognized in culture by its bivalved, winged, spiny ascospores, its aspergillus fischerianus anamorph, and its thermotolerance ...	1992	1624579
incidence of heat-resistant fungi in nsukka, southern nigeria.	a total of 42 soil samples collected from different parts of nsukka, southern nigeria, and 20 freshly plucked mango fruits were screened for the presence of heat-resistant fungi. each soil sample, suspended in sterile water, and washings from each mango fruit were separately heated at 70 degrees c for 1 h before plating on double-strength potato dextrose agar containing chloramphenicol and incubating at approx. 28 degrees c. approximately 98% of all soil samples and 17% of mango fruits contained ...	1991	1909547
neosartorya fischeri var fischeri (wehmer) malloch and cain 1972 (anamorph: aspergillus fischerianus samson and gams 1985) as a cause of mycotic keratitis.	the first case of mycotic keratitis caused by neosartorya fischeri var. fischeri the teleomorph of aspergillus fischerianus, is described. the patient, a 62-year-old man, had no history of trauma to his infected left eye. the infection progressed despite treatment with ketoconazole and the eye had to be eviscerated. histological studies revealed the presence of hyaline, septate mycelium in the eye tissue. cultures gave rise to a thermotolerant mould that developed both its asexual and sexual for ...	1990	2091938
growth of and fumitremorgin production by neosartorya fischeri as affected by temperature, light, and water activity.	the effects of temperature, light, and water activity (aw) on the growth and fumitremorgin production of a heat-resistant mold, neosartorya fischeri, cultured on czapek yeast autolysate agar (cya) were studied for incubation periods of up to 74 days. colonies were examined visually, and extracts of mycelia and cya on which the mold was cultured were analyzed for mycotoxin content by high-performance liquid chromatography. growth always resulted in the production of the tremorgenic mycotoxins ver ...	1988	3415223
growth characteristics of selected fungi on polyvinyl chloride film.	the objective of this study was to determine if plasticized polyvinyl chloride film would support the growth of any of nine species of fungi. the film was suspended in distilled water with no nutrients or with glucose or ammonium sulfate. spores of each of the test species were inoculated into the suspension medium, and the mixture was incubated at 30 degrees c for up to 18 weeks. most species were found to be capable of utilizing the film for carbon or nitrogen when the other nutrient was suppl ...	1986	3707118
verruculogen produced by soil fungi in england and wales.	soil fungi, including aspergillus fischeri, penicillium piceum, penicillium nigricans, and penicillium raistrickii, produced a tremorgenic toxin previously described as toxin x. chemical analysis showed that this toxin was predominantly verruculogen.	1981	7316507
effect of cadmium on fungi and on interactions between fungi and bacteria in soil: influence of clay minerals and ph.	fungi (rhizopus stolonifer, trichoderma viride, fusarium oxysporum f. sp. conglutinans, cunninghamella echinulata, and several species of aspergillus and penicillium) tolerated higher concentrations of cadmium (cd) when grown in soil than when grown on laboratory media, indicating that soil mitigated the toxic effects of cd. in soil amended with clay minerals, montmorillonite provided partial or total protection against fungistatic effects of cd, whereas additions of kaolinite provided little or ...	1977	18085
[comparative enzymatic study of two strains of aspergillus fumigatus and of a. fischeri of sapropytic origin or isolated from human or animal lesions: practical diagnostic applications].	14 enzymatic activities (3 dehydrogenases and 11 hydrolases) were systematically investigated in the extracts of 2 strains of aspergillus fischeri and 14 strains of a. fumigatus (8 isolated from human or animal lesions named 'pathogenic' strains and 6 strains of saprophytic origin). the enzymic composition of a. fumigatus appeared to be very different from one strain to another but no relationships could be established between enzymic differences and origin of the strains. in addition, the immun ...	1976	792691
pulmonary aspergillosis caused by aspergillus fischeri var. spinosus: report of a case and value of serologic studies.		1973	4202251
isolation and characterization of antigens of aspergillus fischeri.		1971	5005720
incidence of heat-resistant molds in eastern orchards and vineyards.		1971	5544294
the transformation of progesterone by cladosporium cladosporioides and aspergillus fischeri.		1970	5528374
a new variety of aspergillus fischeri.		1968	5700469
the chemistry of mould tissue: factors influencing the amount and nature of the fat produced by aspergillus fischeri.		1935	16745649
the chemistry of mould tissue: fractionation of the nitrogen in the mycelium of aspergillus fischeri.		1934	16745412