Publications

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first detection and molecular identification of babesia gibsoni from rhipicephalus sanguineus ticks.babesia gibsoni was firstly identified in rhipicephalus sanguineus ticks infested on dogs in asia. a total of 1195 ticks collected from veterinary practitioners and pet clinics were examined for babesia by nested-polymerase chain reaction assay based on the 18s small subunit ribosomal rna (ssrrna) gene. babesia infection was detected in nymph, male and female ticks with an infection rate of 2.42%, 0.98% and 1.97%, respectively. sequence and phylogenetic analysis revealed that these babesia spp. ...201727686959
molecular investigation into the presence of a coxiella sp. in rhipicephalus sanguineus ticks in australia.q fever is an infectious disease with a global distribution caused by the intracellular bacterium, coxiella burnetii, which has been detected in a large number of tick species worldwide, including the brown dog tick, rhipicephalus sanguineus. recent reports of a high seroprevalance of c. burnetii in australian dogs, along with the identification of additional coxiella species within r. sanguineus ticks, has prompted an investigation into the presence and identification of coxiella species in r. ...201728284601
ferritin 1 silencing effect in rhipicephalus sanguineus sensu lato (acari: ixodidae) during experimental infection with ehrlichia canis.rhipicephalus sanguineus sensu lato (s.l.) is a very common ectoparasite of domestic dogs able to transmit several pathogens of human and veterinary importance. tick infestations and tick-borne diseases (tbds) remain a serious and persistent problem, due to the lack of efficient control measures. it is therefore vital that novel approaches to control are pursued. whilst vaccination is recognised as a potential control method to reduce tick infestation, no anti-r. sanguineus vaccine is available. ...201727825659
detection and molecular identification of hepatozoon canis and babesia vogeli from domestic dogs in palestine.dogs serve as hosts for a great number of parasites, which may affect their health and wellbeing. this study aimed to observe tick borne pathogens in dogs from palestine including hepatozoon canis and babesia species. the prevalence of both h. canis and babesia species infections in apparently healthy dogs, from ten districts of the west bank was surveyed. dna was extracted from blood samples obtained from dogs (n = 362) and ticks (n = 213) collected from dogs (n = 77). a primer set that amplifi ...201727938422
retrospective molecular study on canine hepatozoonosis in slovakia - does infection risk for dogs really exist?this study provides the very first evidence of hepatozoon canis infection in dogs from slovakia, a rhipicephalus sanguineus sensu lato free area. in total, 297 red foxes (vulpes vulpes) and 293 dogs, from three regions of slovakia, were screened for the presence of h. canis using a conventional 18s rrna polymerase chain reaction (pcr). genomic dna of this blood parasite was detected in 51 (17.1%) foxes from all sampling regions in slovakia, whereas the overall prevalence in examined dogs was sig ...201728366618
feline and canine leishmaniosis and other vector-borne diseases in the aeolian islands: pathogen and vector circulation in a confined environment.vector-borne diseases (vbds) are prevalently investigated in dogs. studies on feline vbds are scant, though feline leishmaniosis (fel) is increasingly recognised as a disease of cats in endemic areas. comprehensive investigations on the distribution of vbds in populations of cats and dogs living in relatively small geographical areas, such as islands, are currently lacking. in this study the prevalence of leishmania infantum and other vbd pathogens was assessed in cohorts of cats and dogs living ...201728288759
heat-stressed metarhizium anisopliae: viability (in vitro) and virulence (in vivo) assessments against the tick rhipicephalus sanguineus.the current study investigated the thermotolerance of metarhizium anisopliae s.l. conidia from the commercial products metarril® sp organic and metarril® wp. the efficacy of these m. anisopliae formulations against the tick rhipicephalus sanguineus s.l. was studied in laboratory under optimum or heat-stress conditions. the products were prepared in water [tween® 80, 0.01 % (v/v)] or pure mineral oil. conidia from metarril® sp organic suspended in water presented markedly delayed germination afte ...201727704216
detection of rickettsia hoogstraalii, rickettsia helvetica, rickettsia massiliae, rickettsia slovaca and rickettsia aeschlimannii in ticks from sardinia, italy.tick-borne diseases represent a large proportion of infectious diseases that have become a world health concern. the presence of rickettsia spp. was evaluated by standard pcr and sequencing in 123 ticks collected from several mammals and vegetation in sardinia, italy. this study provides the first evidence of the presence of rickettsia hoogstralii in haemaphysalis punctata and haemaphysalis sulcata ticks from mouflon and rickettsia helvetica in ixodes festai ticks from hedgehog. in addition, ric ...201728110915
genetic characterization of spotted fever group rickettsiae in questing ixodid ticks collected in israel and environmental risk factors for their infection.this study aimed to genetically characterize spotted fever group rickettsiae (sfgr) in questing ixodid ticks from israel and to identify risk factors associated with sfgr-positive ticks using molecular techniques and geographic information systems (gis) analysis. 1039 ticks from the genus rhipicephalus were collected during 2014. 109/1039 (10·49%) carried sfgr-dna of either rickettsia massiliae (95), 'candidatus rickettsia barbariae' (8) or rickettsia conorii (6). higher prevalence of sfgr was f ...201728330517
mediterranean spotted fever: a rare non-endemic disease in the usa.we report a case of a 43-year-old israeli male who presented with an intermittent fever associated with a gradual appearance of diffusely scattered erythematous non-pruritic maculopapular lesions, generalized body malaise, muscle aches, and distal extremity weakness. he works in the israeli military and has been exposed to dogs that are used to search for people in tunnels and claimed that he had removed ticks from the dogs. in the hospital, he presented with fever, a diffuse maculopapular rash, ...201728191378
"candidatus rickettsia asemboensis" in rhipicephalus sanguineus ticks, brazil."candidatus rickettsia asemboensis" is an obligate intracellular bacterium of the rickettsiales order, genetically related to species belonging to the rickettsia felis group, agents of flea-borne spotted fever. here we report for the first time the detection of "ca. r. asemboensis", a flea-associated organism, in rhipicephalus sanguineus ticks. it is the first occurrence of this emerging bacterium in brazil, which increases the geographical distribution of this r. felis-like agent.201727986544
synanthropic mammals as potential hosts of tick-borne pathogens in panama.synanthropic wild mammals can be important hosts for many vector-borne zoonotic pathogens. the aim of this study was determine the exposure of synanthropic mammals to two types of tick-borne pathogens in panama, spotted fever group rickettsia (sfgr) and borrelia relapsing fever (rf) spirochetes. one hundred and thirty-one wild mammals were evaluated, including two gray foxes, two crab-eating foxes (from zoos), four coyotes, 62 opossum and 63 spiny rats captured close to rural towns. to evaluate ...201728060928
molecular detection of rickettsia species in ticks collected from the southwestern provinces of the republic of korea.rickettsiae constitute a group of arthropod-borne, gram-negative, obligate intracellular bacteria that are the causative agents of diseases ranging from mild to life threatening that impact on medical and veterinary health worldwide.201728069059
molecular ecological insights into neotropical bird-tick interactions.in the tropics, ticks parasitize many classes of vertebrate hosts. however, because many tropical tick species are only identifiable in the adult stage, and these adults usually parasitize mammals, most attention on the ecology of tick-host interactions has focused on mammalian hosts. in contrast, immature neotropical ticks are often found on wild birds, yet difficulties in identifying immatures hinder studies of birds' role in tropical tick ecology and tick-borne disease transmission. in panama ...201627203693
rickettsia felis, an emerging flea-borne rickettsiosis.rickettsia felis is an emerging insect-borne rickettsial pathogen and the causative agent of flea-borne spotted fever. first described as a human pathogen from the usa in 1991, r. felis is now identified throughout the world and considered a common cause of fever in africa. the cosmopolitan distribution of this pathogen is credited to the equally widespread occurrence of cat fleas (ctenocephalides felis), the primary vector and reservoir of r. felis. although r. felis is a relatively new member ...201627340613
comparative efficacy of oral administrated afoxolaner (nexgard™) and fluralaner (bravecto™) with topically applied permethrin/imidacloprid (advantix(®)) against transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs.the ability of the topical spot-on advantix(®) (50 % permethrin/10 % imidacloprid) to prevent transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs has previously been reported. the recent market introduction of chewable tablets containing the novel compounds, afoxolaner (nexgard™) and fluralaner (bravecto™) enabled us to conduct a comparative efficacy study with respect to the ability of these three products to block transmission of e. canis by ticks to dogs. the sp ...201627317101
virulence genes of rickettsia rickettsii are differentially modulated by either temperature upshift or blood-feeding in tick midgut and salivary glands.rickettsia rickettsii, the etiological agent of rocky mountain spotted fever, is transmitted to humans by ticks. during tick feeding, r. rickettsii is exposed to both temperature elevation and components of the blood meal, which have previously been associated with the reactivation of its virulence. these environmental stimuli were also reported to modulate virulence genes of r. rickettsii infecting a set of organs of adult females of its natural vector, amblyomma aureolatum.201627287539
risk factors for bartonella species infection in blood donors from southeast brazil.bacteria from the genus bartonella are emerging blood-borne bacteria, capable of causing long-lasting infection in marine and terrestrial mammals, including humans. bartonella are generally well adapted to their main host, causing persistent infection without clinical manifestation. however, these organisms may cause severe disease in natural or accidental hosts. in humans, bartonella species have been detected from sick patients presented with diverse disease manifestations, including cat scrat ...201626999057
first identification and description of rickettsioses and q fever as causes of acute febrile illness in nicaragua.rickettsial infections and q fever present similarly to other acute febrile illnesses, but are infrequently diagnosed because of limited diagnostic tools. despite sporadic reports, rickettsial infections and q fever have not been prospectively studied in central america.201628036394
host body size and the diversity of tick assemblages on neotropical vertebrates.identifying the factors that influence the species diversity and distribution of ticks (acari: ixodida) across vertebrate host taxa is of fundamental ecological and medical importance. host body size is considered one of the most important determinants of tick abundance, with larger hosts having higher tick burdens. the species diversity of tick assemblages should also be greater on larger-bodied host species, but empirical studies testing this hypothesis are lacking. here, we evaluate this rela ...201627812506
host specificity in a diverse neotropical tick community: an assessment using quantitative network analysis and host phylogeny.host specificity is a fundamental determinant of tick population and pathogen transmission dynamics, and therefore has important implications for human health. tick host specificity is expected to be particularly high in the tropics, where communities of ticks, hosts and pathogens are most diverse. yet the degree to which tropical tick species are host-specific remains poorly understood. combining new field data with published records, we assessed the specificity of tick-host associations in pan ...201627357506
brazilian spotted fever with an approach in veterinary medicine and one health perspective.there is increasing interaction between man and pathogens transmitted by arthropods, especially by ticks. it is on this background that a holistic approach stands out, for the sake of public health. brazilian spotted fever is an endemic disease at the country's southeast, with amblyomma sculptum as its major contributor, followed by a. aureolatum and potentially rhipicephalus sanguineus. dogs have been considered sentinels, and in some areas the disease in dogs can precede human disease. conside ...201626881183
growth of ehrlichia canis, the causative agent of canine monocytic ehrlichiosis, in vector and non-vector ixodid tick cell lines.canine monocytic ehrlichiosis is caused by ehrlichia canis, a small gram-negative coccoid bacterium that infects circulating monocytes. the disease is transmitted by the brown dog tick rhipicephalus sanguineus s.l. and is acknowledged as an important infectious disease of dogs and other members of the family canidae worldwide. e. canis is routinely cultured in vitro in the canine monocyte-macrophage cell line dh82 and in non-vector ixodes scapularis tick cell lines, but not in cells derived from ...201626837859
morphological and molecular identification of the brown dog tick rhipicephalus sanguineus and the camel tick hyalomma dromedarii (acari: ixodidae) vectors of rickettsioses in egypt.rickettsioses have an epidemiological importance that includes pathogens, vectors, and hosts. the dog tick rhipicephalus sanguineus and the camel tick hyalomma dromedarii play important roles as vectors and reservoirs of rickettsiae. the aim of this study was to determine the prevalence of rickettsiae in ixodid ticks species infesting dogs and camels in egypt, in addition to, the morphological and molecular identification of r. sanguineus and h. dromedarii.201627847418
kampinos national park: a risk area for spotted fever group rickettsioses, central poland?ixodid ticks are important vectors of a variety of bacterial and protozoan pathogens which cause infections in humans. in this study, altogether 1041 questing ixodes ricinus (n = 305) and dermacentor reticulatus ticks (n = 736), sympatrically occurring in kampinos national park (kpn), central-east poland, were analyzed by pcr for rickettsia species. overall, the pathogen prevalence in ticks was 27.5 % for i. ricinus and 42.8 % for d. reticulatus. sequencing analysis showed that the first tick sp ...201627631765
an evidence-based review on medicinal plants used as insecticide and insect repellent in traditional iranian medicine.insects can be the cause of major ecological problems; they can transmit microbes and parasites that affect humans, and damage food crops, trees, and homes. the total economic cost of insect-related damage and disease is immeasurable. in traditional iranian medicine (tim), several medicinal plants have been identified as insecticides or insect repellents, but many of them are still unknown. therefore, the aim of this study was to review the insecticidal or insect repellent activity of certain me ...201627186389
effect of the saliva from different triatomine species on the biology and immunity of tlr-4 ligand and trypanosoma cruzi-stimulated dendritic cells.triatomines are blood-sucking vectors of trypanosoma cruzi, the causative agent of chagas disease. during feeding, triatomines surpass the skin host response through biomolecules present in their saliva. dendritic cells (dcs) play a crucial role in the induction of the protection to aggressive agents, including blood-sucking arthropods. here, we evaluated if salivary components of triatomines from different genera evade the host immunity by modulating the biology and the function of lps- or t. c ...201627938380
an update on the epidemiological situation of spotted fever in brazil.spotted fever is a tick-borne rickettsial disease. in brazil, its notification to the ministry of health is compulsory. since 2007, cases of spotted fever have been integrated to the notifiable diseases information system, and epidemiological analyzes are part of the routines on surveillance programs.201627555867
rangelia vitalii in a free-ranging maned wolf (chrysocyon brachyurus) and co-infections.an adult free-ranged female maned wolf was rescued from a periurban area subject to anthropogenic disturbances in the minas gerais, brazil. the animal presented poor body condition and anemia. the clinical condition rapidly deteriorated culminating in dead and a necropsy was performed. the main gross lesions were marked anemia and blood content in the intestines accompanied by many types of parasites. the protozoa rangelia vitalii was identified by histopathological analysis predominantly within ...201627761403
effect of amblyomma maculatum (acari: ixodidae) saliva on the acute cutaneous immune response to rickettsia parkeri infection in a murine model.rickettsia parkeri luckman (rickettsiales: rickettsiaceae) is a pathogenic spotted fever group rickettsia transmitted by amblyomma maculatum koch (acari: ixodidae) in the united states. the acute innate immune response to this pathogen and the effect of tick feeding or salivary components on this response is largely unknown. we hypothesized that a. maculatum saliva enhances r. parkeri infection via downregulation of the acute cellular and cytokine immune response. c3h/hen mice were intradermally ...201627521760
comparative speed of kill of sarolaner (simparica™ chewables) and fluralaner (bravecto(®)) against induced infestations of amblyomma americanum on dogs.the lone star tick, amblyomma americanum, infests dogs and cats in north america and transmits the pathogens ehrlichia chaffeensis and ehrlichia ewingii, which cause monocytic and granulocytic ehrlichiosis in dogs and humans, and cytauxzoon felis which causes cytauxzoonosis in cats. a parasiticide's speed of kill is important to minimize the direct deleterious effects [related to blood-feeding] of tick infestation and reduce the risk of transmission of tick-borne pathogens. in this study the spe ...201627430425
evasins: therapeutic potential of a new family of chemokine-binding proteins from ticks.blood-sucking parasites, such as ticks, remain attached to their hosts for relatively long periods of time in order to obtain their blood meal without eliciting an immune response. one mechanism used to avoid rejection is the inhibition of the recruitment of immune cells, which can be achieved by a class of chemokine-binding proteins (ckbps) known as evasins. we have identified three distinct evasins produced by the salivary glands of the common brown dog tick, rhipicephalus sanguineus. they dis ...201627375615
comparative speed of kill of oral treatments with simparica™ (sarolaner) and bravecto®(fluralaner) against induced infestations of rhipicephalus sanguineus on dogs.rhipicephalus sanguineus is the most widely distributed tick species infesting dogs worldwide, which may cause discomfort to the host and transmit diseases. acaricides with a rapid and sustained speed of kill are thus important to prevent infestation and to reduce the risk of disease transmission. in this study, the speed of kill of a monthly administered simparica™ (sarolaner) treatment against induced infestations with r. sanguineus on dogs was evaluated and compared with a single dose of brav ...201626911244
small regulatory rnas of rickettsia conorii.small regulatory rnas comprise critically important modulators of gene expression in bacteria, yet very little is known about their prevalence and functions in rickettsia species. r. conorii, the causative agent of mediterranean spotted fever, is a tick-borne pathogen that primarily infects microvascular endothelium in humans. we have determined the transcriptional landscape of r. conorii during infection of human microvascular endothelial cells (hmecs) by strand-specific rna sequencing to ident ...201627834404
identification, distribution and population dynamics of francisella-like endosymbiont in haemaphysalis doenitzi (acari: ixodidae).francisella-like endosymbionts (fles) with significant homology to francisella tularensis (γ-proteobacteria) have been characterized in several tick species, whereas knowledge on their distribution and population dynamics in ticks remains meager. hence, in the current study, we identified a novel francisella-like endosymbiont (fles-hd) from the tick haemaphysalis doenitzi and evaluated the putative functions of this symbiont. results indicated that fles-hd had 100% infection rate and a perfect v ...201627731377
spotted fever group rickettsiae in ticks of migratory birds in romania.birds are important hosts and dispersers of parasitic arthropods and vector-borne zoonotic pathogens. particularly migratory species may carry these parasites over long distances in short time periods. migratory hotspots present ideal conditions to get a snapshot of parasite and pathogen diversity of birds migrating between continents. the aim of this study was to investigate the presence and diversity of rickettsia spp. in ticks collected from birds at a migratory hot-spot in the danube delta, ...201627207258
candidatus coxiella massiliensis infection.bacteria genetically related to coxiella burnetii have been found in ticks. using molecular techniques, we detected coxiella-like bacteria, here named candidatus coxiella massiliensis, in skin biopsy samples and ticks removed from patients with an eschar. this organism may be a common agent of scalp eschar and neck lymphadenopathy after tick bite.201626811945
rickettsial disease in the peruvian amazon basin.using a large, passive, clinic-based surveillance program in iquitos, peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. blood specimens from humans and animals were assayed for spotted fever group rickettsiae (sfgr) and typhus group rickettsiae (tgr) by elisa and/or pcr; ectoparasites were screened by pcr. logistic regression was used ...201627416029
sero-epidemiological survey of crimean-congo hemorrhagic fever virus in tunisia.crimean-congo hemorrhagic fever (cchf) is a tick-borne disease associated with a high case fatality rate and transmitted mainly by hyalomma marginatum. the geographical distribution of h. marginatum covers most of the western mediterranean basin. we aimed to investigate whether cchf virus (cchfv) is circulating in tunisia. samples from unexplained acute febrile patients (n = 181) and a high risk group of humans, mainly slaughter workers (n = 38), were collected in the summer of 2014 and analyzed ...201626956221
safety evaluation of parastar(®) plus in dogs and assessment of transferable residue of fipronil and cyphenothrin from dogs to humans.dogs are easily infested with fleas, ticks, and other ectoparasites serving as vectors for transmitting bacterial, viral, and parasitic diseases. therefore, the use of ectoparasiticides is inevitable and important. the present investigation was undertaken with two specific objectives: one, to evaluate the safety of fipronil and cyphenothrin in dogs after topical application of parastar(®) plus, and two, to determine the transferable residue of these insecticides from dogs to humans. six healthy, ...201627747219
molecular detection and characterization of zoonotic and veterinary pathogens in ticks from northeastern china.tick-borne diseases are considered as emerging infectious diseases in humans and animals in china. in this study, ixodes persulcatus (n = 1699), haemaphysalis concinna (n = 412), haemaphysalis longicornis (n = 390), dermacentor nuttalli (n = 253), and dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern china, and detected for infection with anaplasma, ehrlichia, babesia, and hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. an ...201627965644
proceedings of the ix international symposium on phlebotomine sandflies (isops ix), reims, france, june 28th-july 1st, 2016. 201627677701
anaplasma species of veterinary importance in japan.anaplasma species of the family anaplasmataceae, order rickettsiales are tick-borne organisms that can cause disease in animals and humans. in japan, all recognized species of anaplasma (except for anaplasma ovis) and a potentially novel anaplasma sp. closely related to anaplasma phagocytophilum have been reported. most of these detected tick-borne pathogens are believed to be lowly pathogenic in animals in japan although the zoonotic a. phagocytophilum has recently been reported to cause clinic ...201627956767
a survey of ticks (acari: ixodidae) of companion animals in australia.ticks are among the most important vectors of pathogens affecting companion animals, and also cause health problems such as tick paralysis, anaemia, dermatitis, and secondary infections. twenty ixodid species have previously been recorded on dogs, cats, and horses in australia, including rhipicephalus sanguineus, ixodes holocyclus and haemaphysalis longicornis, which transmit tick-borne diseases. a survey of hard ticks (acari: ixodidae) was conducted during 2012-2015 to investigate tick species ...201627160149
prediction of g protein-coupled receptor encoding sequences from the synganglion transcriptome of the cattle tick, rhipicephalus microplus.the cattle tick, rhipicephalus (boophilus) microplus, is a pest which causes multiple health complications in cattle. the g protein-coupled receptor (gpcr) super-family presents a candidate target for developing novel tick control methods. however, gpcrs share limited sequence similarity among orthologous family members, and there is no reference genome available for r. microplus. this limits the effectiveness of alignment-dependent methods such as blast and pfam for identifying gpcrs from r. mi ...201626922323
detection of bartonella tamiae, coxiella burnetii and rickettsiae in arthropods and tissues from wild and domestic animals in northeastern algeria.in recent years, the scope and importance of emergent vector-borne diseases has increased dramatically. in algeria, only limited information is currently available concerning the presence and prevalence of these zoonotic diseases. for this reason, we conducted a survey of hematophagous ectoparasites of domestic mammals and/or spleens of wild animals in el tarf and souk ahras, algeria.201626791781
tick-borne pathogens of zoonotic and veterinary importance in nigerian cattle.ticks and tick-borne diseases undermine cattle fitness and productivity in the whole of sub-saharan africa, including nigeria. in this west african country, cattle are challenged by numerous tick species, especially during the wet season. consequently, several tbds are known to be endemic in nigerian cattle, including anaplasmosis, babesiosis, cowdriosis and theilerioris (by theileria mutans and theileria velifera). to date, all investigations on cattle tbds in nigeria have been based on cytolog ...201627090756
ectoparasites of stray dogs in mazandaran, gilan and qazvin provinces, north and center of iran.the aim of the present study was to determine the prevalence of ectoparasite infestations in stray dogs in mazandaran, gilan and qazvin provinces in fall and winter in 2013(december to march).201627308294
induction of humoral immune response to multiple recombinant rhipicephalus appendiculatus antigens and their effect on tick feeding success and pathogen transmission.rhipicephalus appendiculatus is the primary vector of theileria parva, the etiological agent of east coast fever (ecf), a devastating disease of cattle in sub-saharan africa. we hypothesized that a vaccine targeting tick proteins that are involved in attachment and feeding might affect feeding success and possibly reduce tick-borne transmission of t. parva. here we report the evaluation of a multivalent vaccine cocktail of tick antigens for their ability to reduce r. appendiculatus feeding succe ...201627589998
arsenophonus nasoniae and rickettsiae infection of ixodes ricinus due to parasitic wasp ixodiphagus hookeri.arsenophonus nasoniae, a male-killing endosymbiont of chalcid wasps, was recently detected in several hard tick species. following the hypothesis that its presence in ticks may not be linked to the direct occurrence of bacteria in tick's organs, we identified a. nasoniae in wasps emerging from parasitised nymphs. we confirmed that 28.1% of ixodiphagus hookeri wasps parasitizing ixodes ricinus ticks were infected by a. nasoniae. moreover, in examined i. ricinus nymphs, a. nasoniae was detected on ...201626901622
ticks infesting domestic dogs in the uk: a large-scale surveillance programme.recent changes in the distribution of tick vectors and the incidence of tick-borne disease, driven variously by factors such as climate change, habitat modification, increasing host abundance and the increased movement of people and animals, highlight the importance of ongoing, active surveillance. this paper documents the results of a large-scale survey of tick abundance on dogs presented to veterinary practices in the uk, using a participatory approach that allows relatively cost- and time-eff ...201627388169
a review of canine babesiosis: the european perspective.canine babesiosis is a significant tick-borne disease caused by various species of the protozoan genus babesia. although it occurs worldwide, data relating to european infections have now been collected for many years. these data have boosted the publication record and increased our working knowledge of these protozoan parasites. both the large and small forms of babesia species (b. canis, b. vogeli, b. gibsoni, and b. microti-like isolates also referred to as "b. vulpes" and "theileria annae") ...201627289223
detection of vector-borne pathogens in cats and their ectoparasites in southern italy.vector-borne pathogens are the subject of several investigations due to the zoonotic concern of some of them. however, limited data are available about the simultaneous presence of these pathogens in cats and their ectoparasites. the aim of the present study was to define the species of ectoparasites found on cats as well as to investigate vector-borne pathogens in cats and their ectoparasites in southern italy.201627160725
evaluation of the in vitro expression of atp binding-cassette (abc) proteins in an ixodes ricinus cell line exposed to ivermectin.ticks are among the most important vectors of pathogens causing human and animal disease. acaricides are used to control tick infestation, although there are increasing reports of resistance. recently, over-expression of atp-binding cassette (abc) transporter proteins (p-glycoproteins, pgp) has been implicated in resistance to the acaricide ivermectin in the ticks rhipicephalus (boophilus) microplus and rhipicephalus sanguineus sensu lato. ixodid tick cell lines have been used to investigate dru ...201627090214
knock-down and speed of kill of a combination of fipronil and permethrin for the prevention of ctenocephalides felis flea infestation in dogs.a topical combination of fipronil + permethrin (frontline tri-act/frontect, merial) has recently been developed to control fleas, ticks, mosquitoes, sandflies and stable flies on dogs. two studies were conducted to assess its speed of kill and knock-down effect on ctenocephalides felis fleas. the combination was compared to either fipronil alone or to a combination of permethrin, dinotefuran, and pyriproxyfen,201626830931
fatal babesia canis canis infection in a splenectomized estonian dog.a previously splenectomized dog from estonia was presented with a sudden lack of appetite and discoloration of the urine. despite supportive therapy, its condition deteriorated dramatically during 1 day. severe thrombocytopenia and high numbers of protozoan hemoparasites were evident in blood smears, and the hematocrit dropped from 46 to 33 %. the dog was euthanized before specific antibabesial treatment was initiated. blood samples from the dog and from two other dogs in the same household test ...201626810086
assessment of the prophylactic speed of kill of frontline tri-act(®) against ticks (ixodes ricinus and rhipicephalus sanguineus) on dogs.the objective of the study was to assess the speed of kill of a single topical treatment with a combination of fipronil and permethrin (frontline tri-act(®)/frontect(®)) against experimental infestations of ixodes ricinus and rhipicephalus sanguineus ticks on dogs. in this parallel group designed, randomised, single centre, controlled efficacy study, 16 healthy adult dogs were allocated to two groups: 8 dogs were treated with the topical combination on day 0 and the other 8 dogs served as untrea ...201626795064
first report of cytauxzoon sp. infection in a domestic cat from portugal.cytauxzoonosis is an emerging and life-threatening tick-borne feline disease caused by haemoprotozoan parasites of the genus cytauxzoon. information regarding epidemiological and clinical presentation of infections by species other than cytauxzoon felis is scant. a case of cytauxzoon sp. infection is described in a 2-year-old mixed breed male domestic cat from portugal, presenting a history of acute lethargy, anorexia and pyrexia.201627160193
hierarchical bayesian spatio-temporal analysis of climatic and socio-economic determinants of rocky mountain spotted fever.this study aims to examine the spatio-temporal dynamics of rocky mountain spotted fever (rmsf) prevalence in four contiguous states of midwestern united states, and to determine the impact of environmental and socio-economic factors associated with this disease. bayesian hierarchical models were used to quantify space and time only trends and spatio-temporal interaction effect in the case reports submitted to the state health departments in the region. various socio-economic, environmental and c ...201626942604
molecular detection and identification of spotted fever group rickettsiae in ticks collected from the west bank, palestinian territories.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group (sfg) rickettsiae. although spotted fever is prevalent in the middle east, no reports for the presence of tick-borne pathogens are available or any studies on the epidemiology of this disease in the west bank. we aimed to identify the circulating hard tick vectors and genetically characterize sfg rickettsia species in ixodid ticks from the west bank-palestinian territories.201626771654
seroepidemiological studies of crimean-congo hemorrhagic fever virus in domestic and wild animals.crimean-congo hemorrhagic fever (cchf) is a widely distributed, tick-borne viral disease. humans are the only species known to develop illness after cchf virus (cchfv) infection, characterized by a nonspecific febrile illness that can progress to severe, often fatal, hemorrhagic disease. a variety of animals may serve as asymptomatic reservoirs of cchfv in an endemic cycle of transmission. seroepidemiological studies have been instrumental in elucidating cchfv reservoirs and in determining endem ...201626741652
associated factors to seroprevalence of ehrlichia spp. in dogs of quintana roo, mexico.the objective of this study was to determine the seroprevalence to ehrlichia spp. in dogs from xcalak, quintana roo, mexico, and the associated factors. serum samples were obtained from 118 dogs and used in an indirect immunofluorescent assay test for the detection of antibodies against ehrlichia spp. a questionnaire was used to obtain information about possible variables associated with seroprevalence. these variables were analyzed through chi(2) test and logistic regression. dog seroprevalence ...201628096818
exon-intron structure and sequence variation of the calreticulin gene among rhipicephalus sanguineus group ticks.calreticulin proteins (crts) are important components of tick saliva, which is involved in the blood meal success, pathogen transmission and host allergic responses. the characterization of the genes encoding for salivary proteins, such as crts, is pivotal to understand the mechanisms of tick-host interaction during blood meal and to develop tick control strategies based on their inhibition. in hard ticks, crt genes were shown to have only one intron with conserved position among species. in thi ...201627955695
a rare case of mediterranean spotted fever and encephalitis.mediterranean spotted fever is a tick-borne zoonotic disease caused by rickettsia conorii. it is transmitted by the dog tick rhipicephalus sanguineus. it usually presents as a benign self-limited disease characterized by a skin rash, high fever, and, sometimes, a characteristic ulcer at the tick bite site called tache noir. the course of this disease is usually benign, although severe manifestations have been previously described, mainly in adults. neurological manifestations are very unusual. w ...201628053795
a comparative laboratory trial evaluating the immediate efficacy of fluralaner, afoxolaner, sarolaner and imidacloprid + permethrin against adult rhipicephalus sanguineus (sensu lato) ticks attached to dogs.acaricides are used to treat and prevent tick infestations, and a common clinical scenario is to administer an acaricide on observing an attached tick. consequently, immediate acaricidal efficacy (onset of activity and speed of kill) results are clinically valuable. this study evaluated the immediate efficacy of four commercially available acaricides against adult rhipicephalus sanguineus (sensu lato).201627912787
the role of cc-chemokines in the regulation of angiogenesis.angiogenesis, the formation of new blood vessels, is critical for survival and in the regenerative response to tissue injury or ischemia. however, in diseases such as cancer and atherosclerosis, inflammation can cause unregulated angiogenesis leading to excessive neovascularization, which exacerbates disease. current anti-angiogenic therapies cause complete inhibition of both inflammatory and ischemia driven angiogenesis causing a range of side effects in patients. specific inhibition of inflamm ...201627834814
canine and ovine tick-borne pathogens in camels, nigeria.in april 2008, whole blood samples were collected from 36 dromedary camels in sokoto, north-western nigeria. following pcr and reverse line blotting, twenty-two samples (61%) resulted positive for ehrlichia/anaplasma spp. and three (8%) for theileria/babesia spp., with three (8%) cases of co-infections being found. both sequence and blast analyses identified ehrlichia/anaplasma spp. and theileria/babesia spp. positive cases as anaplasma platys and theileria ovis, respectively. this is the first ...201627692338
the intracellular bacterium anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector ixodes scapularis.the intracellular bacteria anaplasma phagocytophilum are emerging zoonotic pathogens affecting human and animal health, and a good model for the study of tick-host-pathogen interactions. this tick-borne pathogen is transmitted by ixodes scapularis in the united states where it causes human granulocytic anaplasmosis. tick midguts and salivary glands play a major role during tick feeding and development, and in pathogen acquisition, multiplication and transmission. vertebrate host proteins are fou ...201627561965
canine babesiosis and tick activity monitored using companion animal electronic health records in the uk.recent publications highlighting autochthonous babesia canis infection in dogs from essex that have not travelled outside the uk are a powerful reminder of the potential for pathogen emergence in new populations. here the authors use electronic health data collected from two diagnostic laboratories and a network of 392 veterinary premises to describe canine babesia cases and levels of babesia concern from january 2015 to march 2016, and the activity of ticks during december 2015-march 2016. in m ...201627484328
artisanal extraction and traditional knowledge associated with medicinal use of crabwood oil (carapa guianensis aublet.) in a peri-urban várzea environment in the amazon estuary.várzea forests of the amazon estuary contain species of importance to riverine communities. for example, the oil extracted from the seeds of crabwood trees is traditionally used to combat various illnesses and as such artisanal extraction processes have been maintained. the objectives of this study were to (1) describe the process involved in artisanal extraction of crabwood oil in the fazendinha protected area, in the state of amapá; (2) characterise the processes of knowledge transfer associat ...201627478479
efficacy of fluralaner spot-on solution against induced infestations with rhipicephalus sanguineus on dogs.the efficacy of fluralaner spot-on solution administered once topically against induced infestations with rhipicephalus sanguineus was evaluated in dogs over a 12-week post-treatment period.201627241176
anthelmintic effect of bacillus thuringiensis strains against the gill fish trematode centrocestus formosanus.parasitic agents, such as helminths, are the most important biotic factors affecting aquaculture, and the fluke centrocestus formosanus is considered to be highly pathogenic in various fish species. there have been efforts to control this parasite with chemical helminthicides, but these efforts have had unsuccessful results. we evaluated the anthelmintic effect of 37 strains of bacillus thuringiensis against c. formosanus metacercariae in vitro using two concentrations of total protein, and only ...201627294137
efficacy and speed of kill of a topically applied formulation of dinotefuran-permethrin-pyriproxyfen against weekly tick infestations with rhipicephalus sanguineus (sensu lato) on dogs.rhipicephalus sanguineus (sensu lato) is a vector of canine babesiosis, anaplasmosis and ehrlichiosis. in order to reduce the chance of transmission of these diseases, an ectoparasiticide should rapidly repel or kill new infestations with this tick. the primary objective of the present study was to evaluate the treatment and preventive acaricidal efficacy of vectra® 3d (54.45 mg/ml of dinotefuran, 396.88 mg/ml of permethrin and 4.84 mg/ml of pyriproxyfen) against r. sanguineus (s.l.) measured at ...201627183970
molecular investigation of tick-borne pathogens in dogs from luanda, angola.no molecular data have been available on tick-borne pathogens that infect dogs from angola. the occurrence of agents from the genera anaplasma, babesia, ehrlichia and hepatozoon was assessed in 103 domestic dogs from luanda, by means of the polymerase chain reaction (pcr) and dna sequence analysis.201627160839
canine vector-borne pathogens in semi-domesticated dogs residing in northern cambodia.in southeast asia, the canine vector-borne pathogens babesia spp., ehrlichia canis, anaplasma platys, hepatozoon canis, haemotropic mycoplasmas and dirofilaria immitis cause significant morbidity and mortality in dogs. moreover, dogs have also been implicated as natural reservoirs for rickettsia felis, the agent of flea-borne spotted fever, increasingly implicated as a cause of undifferentiated fever in humans in southeast asia. the objective of this study was to determine the prevalence and div ...201627161452
vertical transmission of anaplasma platys and leishmania infantum in dogs during the first half of gestation.leishmania infantum is a canine zoonotic vector-borne protozoan pathogen transmitted by phlebotomine sand flies, whereas anaplasma platys is a bacterium most likely transmitted by ticks. while vertical transmission of l. infantum from pregnant bitches to their offspring has been documented, thus far no studies have explored the possibility of vertical transmission of a. platys in dogs. this study investigated the occurrence of vertical transmission of l. infantum and a. platys in sheltered dogs ...201627161003
efficacy of a novel topical combination of fipronil 9.8% and (s)-methoprene 8.8% against ticks and fleas in naturally infested dogs.the efficacy of a novel topical combination of fipronil 9.8% (w/v) and (s)-methoprene 8.8% (w/v) (fiprofort® plus) was tested against ticks and fleas in naturally infested dogs. a total of fifty dogs were allocated in the study with ticks infestation (n = 35) and fleas infestation (n = 15). on day 0, thirty-five tick and fifteen flea infested dogs received the test formulation, a combination of fipronil 9.8% (w/v) and (s)-methoprene 8.8% (w/v) spot-on solution. ticks and flea counts were taken o ...201627190688
rhipicephalus sanguineus (acari: ixodidae) biting a human being in portoalegre city, rio grande do sul, brazil.we report the finding of a female brown dog tick, rhipicephalus sanguineus (acari: ixodidae) on the scalp of a male patient inporto alegre, rio grande do sul, brazil. human parasitism by this tick is rare and has seldomly been reported in the literature, despite its recognized importance since it can act as a vector of rickettsia rickettsii, the agent of spotted fever.201627074329
deltamethrin-mediated toxicity and cytomorphological changes in the midgut and nervous system of the mayfly callibaetis radiatus.immature instars of mayflies are important constituents of the food web in aquatic ecosystems (especially in neotropical regions) and they are among the most susceptible arthropods to pyrethroid insecticides. these insecticides have been recognized as important stressors of freshwater ecosystems, but their cellular effects in aquatic insects have been neglected. here, we assessed the susceptibility to deltamethrin (a typical type ii pyrethroid) as well as the deltamethrin-mediated cytomorphologi ...201627031238
molecular detection of anaplasma platys infection in free-roaming dogs and ticks from kenya and ivory coast.anaplasma platys is a bacterium parasitic in the canine platelets, representing the causative agent of canine cyclic thrombocytopenia, with a worldwide distribution, mainly in tropical countries. the agent has zoonotic potential, being reported in several human clinical cases. the suspected vector is the tick rhipicephalus sanguineus (sensu lato), widely distributed in animals and humans in the tropical regions of south america, africa, asia and australia, but also in southern europe. only few s ...201626984538
comparative speed of kill of sarolaner (simparica) and afoxolaner (nexgard) against induced infestations of amblyomma americanum on dogs.the lone star tick, amblyomma americanum, infests dogs and cats in north america and is the vector of the pathogens that cause monocytic and granulocytic ehrlichiosis in dogs and humans. a parasiticide's speed of kill is important to minimize the direct and deleterious effects of tick infestation and especially to reduce the risk of transmission of tick-borne pathogens. in this study, speed of kill of a novel orally administered isoxazoline parasiticide, sarolaner (simparica chewable tablets), a ...201626897175
comparative speed of kill of sarolaner (simparica) and afoxolaner (nexgard) against induced infestations of rhipicephalus sanguineus s.l. on dogs.the brown dog tick, rhipicephalus sanguineus sensu lato, commonly infests dogs globally, is the major vector of the pathogen that causes canine monocytic ehrlichiosis and also transmits babesia vogeli. a rapid speed of kill of a parasiticide is essential to reduce the direct deleterious effects of tick infestation and the risk of tick-borne pathogen transmission. the speed of kill of a novel orally administered isoxazoline parasiticide, sarolaner (simparica), against r. sanguineus sensu lato on ...201626896456
refractory hypoglycaemia in a dog infected with trypanosoma congolense.a 20 kg german shepherd dog was presented to a french veterinary teaching hospital for seizures and hyperthermia. the dog had returned 1 month previously from a six-month stay in senegal and sub-saharan africa. biochemistry and haematology showed severe hypoglycaemia (0.12 g/l), anaemia and thrombocytopenia. despite administration of large amounts of glucose (30 ml of 30% glucose iv and 10 ml of 70% sucrose by gavage tube hourly), 26 consecutive blood glucose measurements were below 0.25 g/l (ex ...201626795063
retrospective analyses of dogs found serologically positive for ehrlichia canis in cebu, philippines from 2003 to 2014.the study aimed to document the clinical and hematological observations of dogs found serologically positive for ehrlichia canis and to identify parameters or factors that are associated with the disease with focus on the anemic and thrombocytopenic state of the infected dogs.201627051183
molecular assay on crimean congo hemorrhagic fever virus in ticks (ixodidae) collected from kermanshah province, western iran.crimean-congo hemorrhagic fever (cchf) is a feverous and hemorrhagic disease endemic in some parts of iran and caused by an arbovirus related to bunyaviridae family and nairovirusgenus. the main virus reservoir in the nature is ticks, however small vertebrates and a wide range of domestic and wild animals are regarded as reservoir hosts. this study was conducted to determine the infection rate of cchf virus in hard ticks of sarpole-zahab county, kermanshah province, west of iran.201627308296
ehrlichiosis in household dogs and parasitized ticks in kerman-iran: preliminary zoonotic risk assessment.ehrlichiosis is an emerging tick-borne zoonotic disease caused by the family of anaplasmatacea. recently, outbreak of human monocytic ehrlichiosis was reported in northern part of iran. besides, serological evidence of canine monocytic ehrlichiosis caused by ehrlichia canis was reported from southeastern of iran but the epidemiology of this disease is almost undetermined in iran. the present study was designed to use pcr for detection of ehrlichia spp. in tick infested household dogs and determi ...201627308282
gray wolf exposure to emerging vector-borne diseases in wisconsin with comparison to domestic dogs and humans.world-wide concern over emerging vector-borne diseases has increased in recent years for both animal and human health. in the united sates, concern about vector-borne diseases in canines has focused on lyme disease, anaplasmosis, ehrlichiosis, and heartworm which infect domestic and wild canids. of these diseases, lyme and anaplasmosis are also frequently diagnosed in humans. gray wolves (canis lupus) recolonized wisconsin in the 1970s, and we evaluated their temporal and geographic patterns of ...201627898670
ticks (acari: ixodidae) infesting cattle and some other domestic and wild hosts on the french mediterranean island of corsica.corsica is a mountainous french island in the north-western mediterranean presenting a large diversity of natural environments where many interactions between domestic animals and wild fauna occur. despite a favourable context for ticks and tick-borne diseases (tbds), the tick fauna of corsica has not systematically been investigated.201627842608
systemically and cutaneously distributed ectoparasiticides: a review of the efficacy against ticks and fleas on dogs.acaricidal (tick) and insecticidal (flea) efficacy of systemically and cutaneously distributed ectoparasiticide products for dogs are compared based on permethrin and fluralaner as representative molecules. results of efficacy studies against fleas and ticks are reviewed that show generally good to excellent results. both externally and systemically distributed treatments have benefits and weaknesses in potentially preventing pathogen transmission by these arthropod vectors.four general properti ...201627502490
molecular evidence of bartonella species in ixodid ticks and domestic animals in palestine.ticks play an important role in disease transmission as vectors for human and animal pathogens, including the gram-negative pathogen bartonella. here, we evaluated the presence of bartonella in ixodid ticks and domestic animals from palestine. we tested 633 partly engorged ticks and 139 blood samples from domestic animals (dogs, sheep and camels) for bartonella using its-pcr. bartonella dna was detected in 3.9% of the tested ticks. none of the ticks collected from sheep and goats were positive f ...201627540374
identification of a novel β-adrenergic octopamine receptor-like gene (βaor-like) and increased atp-binding cassette b10 (abcb10) expression in a rhipicephalus microplus cell line derived from acaricide-resistant ticks.the cattle tick rhipicephalus (boophilus) microplus is an economically important parasite of livestock. effective control of ticks using acaricides is threatened by the emergence of resistance to many existing compounds. several continuous r. microplus cell lines have been established and provide an under-utilised resource for studies into acaricide targets and potential genetic mutations associated with resistance. as a first step to genetic studies using these resources, this study aimed to de ...201627484910
passive surveillance of ixodes scapularis (say), their biting activity, and associated pathogens in massachusetts.a passive surveillance of tick-borne pathogens was conducted over a 7-year period (2006-2012), in which a total of 3551 ticks were submitted to the university of massachusetts for pcr testing. the vast majority of these ticks were ixodes scapularis from massachusetts (n = 2088) and hence were the focus of further analysis. two taqman duplex qpcr assays were developed to test i. scapularis ticks for the presence of three human pathogens: borrelia burgdorferi, anaplasma phagocytophilum, and babesi ...201627248292
countrywide serological evaluation of canine prevalence for anaplasma spp., borrelia burgdorferi (sensu lato), dirofilaria immitis and ehrlichia canis in mexico.canine vector-borne diseases (cvbd) have become a major concern for canine and human public health. the aim of the study described here is to add epidemiological data regarding four pathogens responsible for cvbd, namely anaplasmosis, borreliosis, dirofilariosis and ehrlichiosis in a national survey conducted in mexico.201627474020
exposure to vector-borne pathogens in candidate blood donor and free-roaming dogs of northeast italy.many vector-borne pathogens including viruses, bacteria, protozoa and nematodes occur in northeast italy, representing a potential threat to animal and human populations. little information is available on the circulation of the above vector-borne pathogens in dogs. this work aims to (i) assess exposure to and circulation of pathogens transmitted to dogs in northeast italy by ticks, sandflies, and mosquitoes, and (ii) drive blood donor screening at the newly established canine blood bank of the ...201627357128
borrelia sp. phylogenetically different from lyme disease- and relapsing fever-related borrelia spp. in amblyomma varanense from python reticulatus.species of the genus borrelia are causative agents of lyme disease and relapsing fever. lyme disease is the most commonly reported vector-borne disease in the northern hemisphere. however, in some parts of the world lyme borreliosis and relapsing fever may be caused by novel borrelia genotypes. herein, we report the presence of a borrelia sp. in an amblyomma varanense collected from python reticulatus.201627342652
dermacentor reticulatus: a vector on the rise.dermacentor reticulatus is a hard tick species with extraordinary biological features. it has a high reproduction rate, a rapid developmental cycle, and is also able to overcome years of unfavourable conditions. dermacentor reticulatus can survive under water for several months and is cold-hardy even compared to other tick species. it has a wide host range: over 60 different wild and domesticated hosts are known for the three active developmental stages. its high adaptiveness gives an edge to th ...201627251148
seroprevalence of vector-borne pathogens and molecular detection of borrelia afzelii in military dogs from portugal.canine vector-borne diseases (cvbds) are increasingly being reported worldwide and represent a serious threat to both animal and public health. military dogs may constitute a risk group for the agents causing these diseases, as they frequently work outdoors in different areas and are thus exposed to vector arthropods. in order to assess the risk of exposure of this type of dogs, a serological and molecular survey was conducted in military working dogs in portugal. one hundred apparently healthy ...201627160284
molecular detection of tick-borne bacteria and protozoa in cervids and wild boars from portugal.wildlife can act as reservoir of different tick-borne pathogens, such as bacteria, parasites and viruses. the aim of the present study was to assess the presence of tick-borne bacteria and protozoa with veterinary and zoonotic importance in cervids and wild boars from the centre and south of portugal.201627160767
factors associated with anaplasma spp. seroprevalence among dogs in the united states.dogs in the united states are hosts to a diverse range of ticks and tick-borne pathogens, including a. phagocytophilum, an important emerging canine and human pathogen. previously, a companion animal parasite council (capc)-sponsored workshop proposed factors purported to be associated with the infection risk for tick-transmitted pathogens in dogs in the united states, including climate conditions, socioeconomic characteristics, local topography, and vector distribution.201627004557
novel spotted fever group rickettsiae in haemaphysalis qinghaiensis ticks from gansu, northwest china.rickettsia spp. are obligate intracellular bacteria and well known as transmitted by arthropods. these pathogens have a broad geographic distribution and a high degree of biological and clinical diversity. this study was conducted to determine the prevalence and molecular characterization of rickettsia spp. in ticks collected from gansu, where borrelia burgdorferi sensu lato and anaplasma phagocytophilum were previously reported in ticks and ruminants.201626968160
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