Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| immunization-induced anaplasma marginale-specific t-lymphocyte responses impaired by a. marginale infection are restored after eliminating infection with tetracycline. | infection of cattle with anaplasma marginale fails to prime sustained effector/memory t-cell responses, and high bacterial load may induce antigen-specific cd4 t exhaustion and deletion. we tested the hypothesis that clearance of persistent infection restores the exhausted t-cell response. we show that infection-induced t-cell exhaustion, characterized as loss of antigen-specific proliferation, and gamma interferon (ifn-γ) and tumor necrosis factor alpha (tnf-α) production are partially restored ... | 2014 | 25008904 |
| murine cutaneous responses to the rocky mountain spotted fever vector, dermacentor andersoni, feeding. | tick salivary glands produce complex cocktails of bioactive molecules that facilitate blood feeding and pathogen transmission by modulating host hemostasis, pain/itch responses, wound healing, and both innate and adaptive immunity. in this study, cutaneous responses at dermacentor andersoni bite-sites were analyzed using affymetrix mouse genome arrays and histopathology at 12, 48, 96 and 120 h post- infestation (hpi) during primary infestations and 120 hpi during secondary infestations. the micr ... | 2014 | 24847317 |
| movement of male dermacentor andersoni (acari: ixodidae) among cattle. | movement of male dermacentor andersoni (stiles) was examined among 54 pairs of artificially infested donor and recipient cattle during a 3-yr period. the number of males declined at a rate independent of the initial level of infestation, while the rate of decline of females on the donor animals tended to increase with initial infestation level. male tick movement to recipient cattle was observed in 26 of 54 (48%) of the animal pairs, but varied among years and trials. movement tended to be great ... | 2013 | 24180101 |
| novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses. | hard ticks subvert the immune responses of their vertebrate hosts in order to feed for much longer periods than other blood-feeding ectoparasites; this may be one reason why they transmit perhaps the greatest diversity of pathogens of any arthropod vector. tick-induced immunomodulation is mediated by salivary components, some of which neutralise elements of innate immunity or inhibit the development of adaptive immunity. as dendritic cells (dc) trigger and help to regulate adaptive immunity, the ... | 2013 | 23825947 |
| protective immunity induced by immunization with a live, cultured anaplasma marginale strain. | despite significant economic losses resulting from infection with anaplasma marginale, a tick-transmitted rickettsial pathogen of cattle, available vaccines provide, at best, only partial protection against clinical disease. the green-fluorescent protein expressing mutant of the a. marginale st. maries strain is a live, marked vaccine candidate (amstm-gfp). to test whether amstm-gfp is safe and provides clinical protection, a group of calves was vaccinated, and clinical parameters, including per ... | 2013 | 23664994 |
| identification of multilocus genetic heterogeneity in anaplasma marginale subsp. centrale and its restriction following tick-borne transmission. | anaplasma marginale subsp. centrale was the first vaccine used to protect against a rickettsial disease and is still in widespread use a century later. as its use preceded development of either cryopreservation or cell culture, the vaccine strain was maintained for decades by sequential passage among donor animals, excluding the natural tick-borne transmission cycle that provides a selective pressure or population "bottleneck." we demonstrated that the vaccine strain is genetically heterogeneous ... | 2013 | 23509140 |
| hitch-hiker taken for a ride: an unusual cause of myocarditis, septic shock and adult respiratory distress syndrome. | rocky mountain spotted fever (rmsf) is a serious tick-borne illness caused by rickettsia rickettsii that is endemic in southeastern usa. although rmsf has been described as causing the classic clinical triad of fever, headache and a characteristic rash, serious and potentially life-threatening manifestations can occur. cardiopulmonary involvement, although infrequent, may occur with severe cases of rmsf. rickettsial myocarditis is an uncommon occurrence. we present a case of a previously healthy ... | 2013 | 23314875 |
| range expansion of dermacentor variabilis and dermacentor andersoni (acari: ixodidae) near their northern distributional limits. | distributional ranges of the ticks dermacentor andersoni stiles and dermacentor variabilis (say) in the canadian prairies were determined by passive surveillance and active collection. these findings were compared with historical records of both species, particularly in the province of saskatchewan, where the northern distributional limits of both tick species occur. before the 1960s, d. variabilis and d. andersoni were allopatric in saskatchewan; however, since then, the distribution of d. vari ... | 2013 | 23802445 |
| ticks (acari: ixodidae) on small mammals in kootenay national park, british columbia, canada. | two hundred and ninety-one ticks (i.e., 185 larvae, 72 nymphs, and 34 adults) were removed from 153 small mammals comprising six species collected in verdant forest, numa forest, and marble canyon within kootenay national park, british columbia, canada. morphological examination and molecular analyses (i.e., polymerase chain reaction-single-strand conformation polymorphism [pcr-sscp] and dna sequencing of the mitochondrial 16s rrna gene) of the ticks revealed that most individuals were ixodes an ... | 2013 | 24843924 |
| transmission of pathogens by stomoxys flies (diptera, muscidae): a review. | stomoxys flies are mechanical vectors of pathogens present in the blood and skin of their animal hosts, especially livestock, but occasionally humans. in livestock, their direct effects are disturbance, skin lesions, reduction of food intake, stress, blood loss, and a global immunosuppressive effect. they also induce the gathering of animals for mutual protection; meanwhile they favor development of pathogens in the hosts and their transmission. their indirect effect is the mechanical transmissi ... | 2013 | 23985165 |
| a novel rickettsia species detected in vole ticks (ixodes angustus) from western canada. | the genomic dna of ixodid ticks from western canada was tested by pcr for the presence of rickettsia. no rickettsiae were detected in ixodes sculptus, whereas 18% of the i. angustus and 42% of the dermacentor andersoni organisms examined were pcr positive for rickettsia. the rickettsiae from each tick species were characterized genetically using multiple genes. rickettsiae within the d. andersoni organisms had sequences at four genes that matched those of r. peacockii. in contrast, the rickettsi ... | 2013 | 24077705 |
| ticks and tick-borne pathogens at the cutaneous interface: host defenses, tick countermeasures, and a suitable environment for pathogen establishment. | ticks are unique among hematophagous arthropods by continuous attachment to host skin and blood feeding for days; complexity and diversity of biologically active molecules differentially expressed in saliva of tick species; their ability to modulate the host defenses of pain and itch, hemostasis, inflammation, innate and adaptive immunity, and wound healing; and, the diverse array of infectious agents they transmit. all of these interactions occur at the cutaneous interface in a complex sequence ... | 2013 | 24312085 |
| a nod to disease vectors: mitigation of pathogen sensing by arthropod saliva. | arthropod saliva possesses anti-hemostatic, anesthetic, and anti-inflammatory properties that facilitate feeding and, inadvertently, dissemination of pathogens. vector-borne diseases caused by these pathogens affect millions of people each year. many studies address the impact of arthropod salivary proteins on various immunological components. however, whether and how arthropod saliva counters nod-like (nlr) sensing remains elusive. nlrs are innate immune pattern recognition molecules involved i ... | 2013 | 24155744 |
| tick salivary compounds: their role in modulation of host defences and pathogen transmission. | ticks require blood meal to complete development and reproduction. multifunctional tick salivary glands play a pivotal role in tick feeding and transmission of pathogens. tick salivary molecules injected into the host modulate host defence responses to the benefit of the feeding ticks. to colonize tick organs, tick-borne microorganisms must overcome several barriers, i.e., tick gut membrane, tick immunity, and moulting. tick-borne pathogens co-evolved with their vectors and hosts and developed m ... | 2013 | 23971008 |
| interaction of the tick immune system with transmitted pathogens. | ticks are hematophagous arachnids transmitting a wide variety of pathogens including viruses, bacteria, and protozoans to their vertebrate hosts. the tick vector competence has to be intimately linked to the ability of transmitted pathogens to evade tick defense mechanisms encountered on their route through the tick body comprising midgut, hemolymph, salivary glands or ovaries. tick innate immunity is, like in other invertebrates, based on an orchestrated action of humoral and cellular immune re ... | 2013 | 23875177 |
| isolation of deer tick virus (powassan virus, lineage ii) from ixodes scapularis and detection of antibody in vertebrate hosts sampled in the hudson valley, new york state. | deer tick virus, dtv, is a genetically and ecologically distinct lineage of powassan virus (powv) also known as lineage ii powv. human incidence of pow encephalitis has increased in the last 15 years potentially due to the emergence of dtv, particularly in the hudson valley of new york state. we initiated an extensive sampling campaign to determine whether powv was extant throughout the hudson valley in tick vectors and/or vertebrate hosts. | 2013 | 24016533 |
| what is the risk for exposure to vector-borne pathogens in united states national parks? | united states national parks attract > 275 million visitors annually and collectively present risk of exposure for staff and visitors to a wide range of arthropod vector species (most notably fleas, mosquitoes, and ticks) and their associated bacterial, protozoan, or viral pathogens. we assessed the current state of knowledge for risk of exposure to vector-borne pathogens in national parks through a review of relevant literature, including internal national park service documents and organismal ... | 2013 | 23540107 |
| endogenous tick viruses and modulation of tick-borne pathogen growth. | ticks transmit a wide range of viral, bacterial and protozoan pathogens, many of which can establish persistent infections of lifelong duration in the vector tick and in some cases are transmitted transovarially to the next generation. in addition many ixodid and argasid tick cell lines and, by inference the parent ticks from which they were derived, harbor endogenous viruses (etv) of which almost nothing is known. in general, low level persistent infections with viral pathogens (arboviruses) ar ... | 2013 | 23875176 |
| hybridization in natural sympatric populations of dermacentor ticks in northwestern north america. | hybridization in ticks has been described in a handful of species and mostly as a result of laboratory experiments. we used 148 aflp loci to describe putative hybridization events between d. andersoni and d. variabilis in sympatric populations from northwestern north america. recently, d. variabilis has expanded its range westward into the natural range of d. andersoni. using a sample of 235 d. andersoni and 62 d. variabilis, we identified 31 individuals as putative hybrids: four f2 individuals ... | 2013 | 23531531 |
| intertwined arbovirus transmission activity: reassessing the transmission cycle paradigm. | arboviruses are emerging/reemerging infectious agents worldwide. the factors within this scenario include vector and host population fluctuations, climatic changes, anthropogenic activities that disturb ecosystems, an increase in international flights, human mobility, and genetic mutations that allow spill-over phenomenon. arboviruses are maintained by biologic transmission among vectors and hosts. sometimes this biological transmission is specific and includes one vector and host species such a ... | 2013 | 23335900 |
| effects of bartonella spp. on flea feeding and reproductive performance. | numerous pathogens are transmitted from one host to another by hematophagous insect vectors. the interactions between a vector-borne organism and its vector vary in many ways, most of which are yet to be explored and identified. these interactions may play a role in the dynamics of the infection cycle. one way to evaluate these interactions is by studying the effects of the tested organism on the vector. in this study, we tested the effects of infection with bartonella species on fitness-related ... | 2013 | 23542614 |
| de novo ixodes ricinus salivary gland transcriptome analysis using two next-generation sequencing methodologies. | tick salivary gland (sg) proteins possess powerful pharmacologic properties that facilitate tick feeding and pathogen transmission. for the first time, sg transcriptomes of ixodes ricinus, an important disease vector for humans and animals, were analyzed using next-generation sequencing. sgs were collected from different tick life stages fed on various animal species, including cofeeding of nymphs and adults on the same host. four cdna samples were sequenced, discriminating tick sg transcriptome ... | 2013 | 23964076 |
| characterization of haemaphysalis flava (acari: ixodidae) from qingling subspecies of giant panda (ailuropoda melanoleuca qinlingensis) in qinling mountains (central china) by morphology and molecular markers. | tick is one of important ectoparasites capable of causing direct damage to their hosts and also acts as vectors of relevant infectious agents. in the present study, the taxa of 10 ticks, collected from qinling giant pandas (ailuropoda melanoleuca qinlingensis) in qinling mountains of china in april 2010, were determined using morphology and molecular markers (nucleotide its2 rdna and mitochondrial 16s). microscopic observation demonstrated that the morphological features of these ticks were simi ... | 2013 | 23894541 |
| comparative evaluation of infected and noninfected amblyomma triste ticks with rickettsia parkeri, the agent of an emerging rickettsiosis in the new world. | the distribution of rickettsia parkeri in south america has been associated with amblyomma triste ticks. the present study evaluated under laboratory conditions two colonies of a. triste: one started from engorged females that were naturally infected by r. parkeri (designated as infected group); the other started from noninfected females (designated as control group). both colonies were reared in parallel for five consecutive generations. tick-naïve domestic rabbits were used for feeding of each ... | 2013 | 23936795 |
| immunoregulation of bovine macrophages by factors in the salivary glands of rhipicephalus microplus. | alternative strategies are required to control the southern cattle tick, rhipicephalus microplus, due to evolving resistance to commercially available acaricides. this invasive ectoparasite is a vector of economically important diseases of cattle such as bovine babesiosis and anaplasmosis. an understanding of the biological intricacies underlying vector-host-pathogen interactions is required to innovate sustainable tick management strategies that can ultimately mitigate the impact of animal and ... | 2012 | 22333193 |
| tick-box for 3'-end formation of mitochondrial transcripts in ixodida, basal chelicerates and drosophila. | according to the trna punctuation model, the mitochondrial genome (mtdna) of mammals and arthropods is transcribed as large polycistronic precursors that are maturated by endonucleolytic cleavage at trna borders and rna polyadenylation. starting from the newly sequenced mtdna of ixodes ricinus and using a combination of mitogenomics and transcriptional analyses, we found that in all currently-sequenced tick lineages (prostriata, metastriata and argasidae) the 3'-end of the polyadenylated nad1 an ... | 2012 | 23077630 |
| the sialotranscriptome of antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food. | the hosts for antricola delacruzi ticks are insectivorous, cave-dwelling bats on which only larvae are found. the mouthparts of nymphal and adult a. delacruzi are compatible with scavenging feeding because the hypostome is small and toothless. how a single blood meal of a larva provides energy for several molts as well as for oviposition by females is not known. adults of a. delacruzi possibly feed upon an unknown food source in bat guano, a substrate on which nymphal and adult stages are always ... | 2012 | 22306723 |
| powassan virus infection: case series and literature review from a single institution. | powassan virus is a flavivirus related to eastern hemisphere's tick-borne encephalitis viruses. it can cause a rare but potentially life-threatening disease including encephalitis. | 2012 | 23111001 |
| detection and identification of putative bacterial endosymbionts and endogenous viruses in tick cell lines. | as well as being vectors of many viral, bacterial, and protozoan pathogens of medical and veterinary importance, ticks harbour a variety of microorganisms which are not known to be pathogenic for vertebrate hosts. continuous cell lines established from ixodid and argasid ticks could be infected with such endosymbiotic bacteria and endogenous viruses, but to date very few cell lines have been examined for their presence. dna and rna extracted from over 50 tick cell lines deposited in the roslin w ... | 2012 | 22743047 |
| the role of nuclear technologies in the diagnosis and control of livestock diseases--a review. | nuclear and nuclear-related technologies have played an important role in animal health, particularly in relation to disease diagnosis and characterization of pathogenic organisms. this review focuses primarily on how and where nuclear technologies, both non-isotopic and isotopic methods, have made their impact in the past and where it might be expected they could have an impact in the future. the review outlines the extensive use of radiation attenuation in attempts to create vaccines for a mul ... | 2012 | 22286376 |
| unusual bacterial infections and the pleura. | rickettsiosis, q fever, tularemia, and anthrax are all bacterial diseases that can affect the pleura. rocky mountain spotted fever (rmsf) and mediterranean spotted fever (msf) are caused by rickettsia rickettsii and rickettsia conorii, respectively. pleural fluid from a patient with msf had a neutrophil-predominant exudate. coxiellaburnetii is the causative agent of q fever. of the two cases described in the literature, one was an exudate with a marked eosinophilia while the other case was a tra ... | 2012 | 22977649 |
| identification of endosymbionts in ticks by broad-range polymerase chain reaction and electrospray ionization mass spectrometry. | many organisms, such as insects, filarial nematodes, and ticks, contain heritable bacterial endosymbionts that are often closely related to transmissible tickborne pathogens. these intracellular bacteria are sometimes unique to the host species, presumably due to isolation and genetic drift. we used a polymerase chain reaction/electrospray ionization-mass spectrometry assay designed to detect a wide range of vectorborne microorganisms to characterize endosymbiont genetic signatures from amblyomm ... | 2012 | 22897044 |
| comparative genomics and transcriptomics of trait-gene association. | the order rickettsiales includes important tick-borne pathogens, from rickettsia rickettsii, which causes rocky mountain spotted fever, to anaplasma marginale, the most prevalent vector-borne pathogen of cattle. although most pathogens in this order are transmitted by arthropod vectors, little is known about the microbial determinants of transmission. a. marginale provides unique tools for studying the determinants of transmission, with multiple strain sequences available that display distinct a ... | 2012 | 23181781 |
| ixodes scapularis saliva mitigates inflammatory cytokine secretion during anaplasma phagocytophilum stimulation of immune cells. | ixodes scapularis saliva enables the transmission of infectious agents to the mammalian host due to its immunomodulatory, anesthetic and anti-coagulant properties. however, how i. scapularis saliva influences host cytokine secretion in the presence of the obligate intracellular rickettsial pathogen anaplasma phagocytophilum remains elusive. | 2012 | 23050849 |
| expansion of variant diversity associated with a high prevalence of pathogen strain superinfection under conditions of natural transmission. | superinfection occurs when a second, genetically distinct pathogen strain infects a host that has already mounted an immune response to a primary strain. for antigenically variant pathogens, the primary strain itself expresses a broad diversity of variants over time. thus, successful superinfection would require that the secondary strain express a unique set of variants. we tested this hypothesis under conditions of natural transmission in both temperate and tropical regions where, respectively, ... | 2012 | 22585962 |
| co2 flagging - an improved method for the collection of questing ticks. | most epidemiological studies on tick-borne pathogens involve collection of ticks from the environment. an efficient collection method is essential for large sample pools. our main aim was to evaluate the efficacy of a new method, where traditional flagging was enhanced by the use of co2 dispersed into the white flannel. the co2 was spread through a rubber hose network inserted into the flag blanket. the research was conducted in spring, in march-april 2011 in two locations from cluj county, roma ... | 2012 | 22720872 |
| why are there so few rickettsia conorii conorii-infected rhipicephalus sanguineus ticks in the wild? | rickettsia conorii conorii is the etiological agent of mediterranean spotted fever, which is transmitted by the brown dog tick, rhipicephalus sanguineus. the relationship between the rickettsia and its tick vector are still poorly understood one century after the first description of this disease. | 2012 | 22724035 |
| composition and seasonal variation of rhipicephalus turanicus and rhipicephalus sanguineus bacterial communities. | a 16s rrna gene approach, including 454 pyrosequencing and quantitative pcr (qpcr), was used to describe the bacterial community in rhipicephalus turanicus and to evaluate the dynamics of key bacterial tenants of adult ticks during the active questing season. the bacterial community structure of rh. turanicus was characterized by high dominance of coxiella and rickettsia and extremely low taxonomic diversity. parallel diagnostic pcr further revealed a novel coxiella species which was present and ... | 2012 | 22467507 |
| tick salivary secretion as a source of antihemostatics. | ticks are mostly obligatory blood feeding ectoparasites that have an impact on human and animal health. in addition to direct damage due to feeding, some tick species serve as the vectors for the causative agents of several diseases, such as the spirochetes of the genus borrelia causing lyme disease, the virus of tick-borne encephalitis, various rickettsial pathogens or even protozoan parasites like babesia spp. hard ticks are unique among bloodfeeders because of their prolonged feeding period t ... | 2012 | 22564820 |
| dispersion and sampling of adult dermacentor andersoni in rangeland in western north america. | a fixed precision sampling plan was developed for off-host populations of adult rocky mountain wood tick, dermacentor andersoni (stiles) based on data collected by dragging at 13 locations in alberta, canada; washington; and oregon. in total, 222 site-date combinations were sampled. each site-date combination was considered a sample, and each sample ranged in size from 86 to 250 10 m2 quadrats. analysis of simulated quadrats ranging in size from 10 to 50 m2 indicated that the most precise sample ... | 2012 | 22493841 |
| subdominant antigens in bacterial vaccines: am779 is subdominant in the anaplasma marginale outer membrane vaccine but does not associate with protective immunity. | identification of specific antigens responsible for the ability of complex immunogens to induce protection is a major goal in development of bacterial vaccines. much of the investigation has focused on highly abundant and highly immunodominant outer membrane proteins. recently however, genomic and proteomic approaches have facilitated identification of minor components of the bacterial outer membrane that have previously been missed or ignored in immunological analyses. immunization with anaplas ... | 2012 | 23029498 |
| tropism and pathogenicity of rickettsiae. | rickettsiae are obligate intracellular parasitic bacteria that cause febrile exanthematous illnesses such as rocky mountain spotted fever, mediterranean spotted fever, epidemic, and murine typhus, etc. although the vector ranges of each rickettsia species are rather restricted; i.e., ticks belonging to arachnida and lice and fleas belonging to insecta usually act as vectors for spotted fever group (sfg) and typhus group (tg) rickettsiae, respectively, it would be interesting to elucidate the mec ... | 2012 | 22737150 |
| parasite diversity and coinfection determine pathogen infection success and host fitness. | while the importance of changes in host biodiversity for disease risk continues to gain empirical support, the influence of natural variation in parasite diversity on epidemiological outcomes remains largely overlooked. here, we combined field infection data from 2,191 amphibian hosts representing 158 parasite assemblages with mechanistic experiments to evaluate the influence of parasite richness on both parasite transmission and host fitness. using a guild of larval trematode parasites (six spe ... | 2012 | 22615371 |
| expression patterns of anaplasma marginale msp2 variants change in response to growth in cattle, and tick cells versus mammalian cells. | antigenic variation of major surface proteins is considered an immune-evasive maneuver used by pathogens as divergent as bacteria and protozoa. likewise, major surface protein 2 (msp2) of the tick-borne pathogen, anaplasma marginale, is thought to be involved in antigenic variation to evade the mammalian host immune response. however, this dynamic process also works in the tick vector in the absence of immune selection pressure. we examined msp2 variants expressed during infection of four tick a ... | 2012 | 22558307 |
| molecular cloning and sequence analysis of the cdnas encoding toxin-like peptides from the venom glands of tarantula grammostola rosea. | tarantula venom glands produce a large variety of bioactive peptides. here we present the identification of venom components obtained by sequencing clones isolated from a cdna library prepared from the venom glands of the chilean common tarantula, grammostola rosea. the cdna sequences of about 1500 clones out of 4000 clones were analyzed after selection using several criteria. forty-eight novel toxin-like peptides (gtx1 to gtx7, and gtx-tctp and gtx-crisp) were predicted from the nucleotide sequ ... | 2012 | 22500178 |
| transovarial transmission of rickettsia spp. and organ-specific infection of the whitefly bemisia tabaci. | the whitefly bemisia tabaci is a cosmopolitan insect pest that harbors portiera aleyrodidarum, the primary obligatory symbiotic bacterium, and several facultative secondary symbionts. secondary symbionts in b. tabaci are generally associated with the bacteriome, ensuring their vertical transmission; however, rickettsia is an exception and occupies most of the body cavity, except the bacteriome. the mode of rickettsia transfer between generations and its subcellular localization in insect organs ... | 2012 | 22660706 |
| q fever: current state of knowledge and perspectives of research of a neglected zoonosis. | q fever is an ubiquitous zoonosis caused by an resistant intracellular bacterium, coxiella burnetii. in certain areas, q fever can be a severe public health problem, and awareness of the disease must be promoted worldwide. nevertheless, knowledge of coxiella burnetii remains limited to this day. its resistant (intracellular and environmental) and infectious properties have been poorly investigated. further understanding of the interactions between the infected host and the bacteria is necessary. ... | 2011 | 22194752 |
| genomotyping of coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates. | c. burnetii is a gram-negative intracellular y-proteobacteria that causes the zoonotic disease q fever. q fever can manifest as an acute or chronic illness. different typing methods have been previously developed to classify c. burnetii isolates to explore its pathogenicity. here, we report a comprehensive genomotyping method based on the presence or absence of genes using microarrays. the genomotyping method was then tested in 52 isolates obtained from different geographic areas, different host ... | 2011 | 22046248 |
| distribution of rickettsia rickettsii in ovary cells of rhipicephalus sanguineus (latreille1806) (acari: ixodidae). | abstract: | 2011 | 22117572 |
| tick cell lines for study of crimean-congo hemorrhagic fever virus and other arboviruses. | abstract continuous cell lines derived from many of the vectors of tick-borne arboviruses of medical and veterinary importance are now available. their role as tools in arbovirus research to date is reviewed and their potential application in studies of tick cell responses to virus infection is explored, by comparison with recent progress in understanding mosquito immunity to arbovirus infection. a preliminary study of propagation of the human pathogen crimean-congo hemorrhagic fever virus (cc ... | 2011 | 21955214 |
| genetics of coxiella burnetii: on the path of specialization. | coxiella burnetii is an extremely infectious, zoonotic agent that causes q fever in humans. with the exception of new zealand, the bacterium is distributed worldwide. coxiella is classified as a select agent based on its past and potential use as a bioweapon and its threat to public health. despite decades of research, we know relatively little regarding coxiella?s molecular pathogenesis, and a vaccine is not widely available. this article briefly reviews the unusual genetics of c. burnetii; a p ... | 2011 | 22082290 |
| characterization of the stop codon readthrough signal of colorado tick fever virus segment 9 rna. | termination codon readthrough is utilized as a mechanism of expression of a growing number of viral and cellular proteins, but in many cases the mrna signals that promote readthrough are poorly characterized. here, we investigated the readthrough signal of colorado tick fever virus (ctfv) segment 9 rna (seg-9). ctfv is the type-species of the genus coltivirus within the family reoviridae and is a tick-borne, double-stranded, segmented rna virus. seg-9 encodes a 36-kda protein vp9, and by readthr ... | 2011 | 22190746 |
| Infection prevalences of common tick-borne pathogens in adult lone star ticks (Amblyomma americanum) and American dog ticks (Dermacentor variabilis) in Kentucky. | Rocky Mountain spotted fever, Lyme disease, and ehrlichiosis are tick-borne diseases that are reported annually in Kentucky. We conducted a survey to describe infection prevalence of tick-borne pathogens in Amblyomma americanum and Dermacentor variabilis ticks collected in Kentucky. During 2007-2008, we collected 287 ticks (179 D. variabilis and 108 A. americanum) from canine, feral hog, horse, raccoon, white-tailed deer, and human hosts in six counties in Kentucky. Ticks were screened for Ricke ... | 2011 | 21976578 |
| a deep insight into the sialotranscriptome of the gulf coast tick, amblyomma maculatum. | saliva of blood sucking arthropods contains compounds that antagonize their hosts' hemostasis, which include platelet aggregation, vasoconstriction and blood clotting; saliva of these organisms also has anti-inflammatory and immunomodullatory properties. perhaps because hosts mount an active immune response against these compounds, the diversity of these compounds is large even among related blood sucking species. because of these properties, saliva helps blood feeding as well as help the establ ... | 2011 | 22216098 |
| a further insight into the sialome of the tropical bont tick, amblyomma variegatum. | ticks--vectors of medical and veterinary importance--are themselves also significant pests. tick salivary proteins are the result of adaptation to blood feeding and contain inhibitors of blood clotting, platelet aggregation, and angiogenesis, as well as vasodilators and immunomodulators. a previous analysis of the sialotranscriptome (from the greek sialo, saliva) of amblyomma variegatum is revisited in light of recent advances in tick sialomes and provides a database to perform a proteomic study ... | 2011 | 21362191 |
| tick paralysis in australia caused by ixodes holocyclus neumann. | ticks are obligate haematophagous ectoparasites of various animals, including humans, and are abundant in temperate and tropical zones around the world. they are the most important vectors for the pathogens causing disease in livestock and second only to mosquitoes as vectors of pathogens causing human disease. ticks are formidable arachnids, capable of not only transmitting the pathogens involved in some infectious diseases but also of inducing allergies and causing toxicoses and paralysis, wit ... | 2011 | 21396246 |
| eschar and neck lymphadenopathy caused by francisella tularensis after a tick bite: a case report. | 2011 | 21418587 | |
| the immunopathology of canine vector-borne diseases. | abstract: the canine vector-borne infectious diseases (cvbds) are an emerging problem in veterinary medicine and the zoonotic potential of many of these agents is a significant consideration for human health. the successful diagnosis, treatment and prevention of these infections is dependent upon firm understanding of the underlying immunopathology of the diseases in which there are unique tripartite interactions between the microorganism, the vector and the host immune system. although signific ... | 2011 | 21489234 |
| one health: the importance of companion animal vector-borne diseases. | abstract: the international prominence accorded the 'one health' concept of co-ordinated activity of those involved in human and animal health is a modern incarnation of a long tradition of comparative medicine, with roots in the ancient civilizations and a golden era during the 19th century explosion of knowledge in the field of infectious disease research. modern one health tends to focus on zoonotic pathogens emerging from wildlife and production animal species, but one of the most significan ... | 2011 | 21489237 |
| experimental infection of amblyomma aureolatum ticks with rickettsia rickettsii. | we experimentally infected amblyomma aureolatum ticks with the bacterium rickettsia rickettsii, the etiologic agent of rocky mountain spotted fever (rmsf). these ticks are a vector for rmsf in brazil. r. rickettsii was efficiently conserved by both transstadial maintenance and vertical (transovarial) transmission to 100% of the ticks through 4 laboratory generations. however, lower reproductive performance and survival of infected females was attributed to r. rickettsii infection. therefore, bec ... | 2011 | 21529391 |
| expression of anaplasma marginale ankyrin repeat-containing proteins during infection of the mammalian host and tick vector. | transmission of tick-borne pathogens requires transition between distinct host environments with infection and replication in host-specific cell types. anaplasma marginale illustrates this transition: in the mammalian host the bacteria infects and replicates in mature (non-nucleated) erythrocytes while in the tick vector replication occurs in nucleated epithelial cells. we hypothesized that proteins containing ankyrin motifs would be expressed by a. marginale only in tick cells and would traffic ... | 2011 | 21576345 |
| francisella tularensis molecular typing using differential insertion sequence amplification (disa). | tularemia is a potentially fatal disease that is caused by the highly infectious and zoonotic pathogen francisella tularensis. despite the monomorphic nature of sequenced f. tularensis genomes, there is a significant degree of plasticity in the organization of genetic elements. the observed variability in these genomes is primarily due to the transposition of direct repeats and insertion sequence (is) elements. since current methods used to genotype f. tularensis are time-consuming and require e ... | 2011 | 21613430 |
| vector competence of the tick ixodes ricinus for transmission of bartonella birtlesii. | bartonella spp. are facultative intracellular vector-borne bacteria associated with several emerging diseases in humans and animals all over the world. the potential for involvement of ticks in transmission of bartonella spp. has been heartily debated for many years. however, most of the data supporting bartonellae transmission by ticks come from molecular and serological epidemiological surveys in humans and animals providing only indirect evidences without a direct proof of tick vector compete ... | 2011 | 21655306 |
| francisella-arthropod vector interaction and its role in patho-adaptation to infect mammals. | francisella tularensis is a gram-negative, intracellular, zoonotic bacterium, and is the causative agent of tularemia with a broad host range. arthropods such as ticks, mosquitoes, and flies maintain f. tularensis in nature by transmitting the bacteria among small mammals. while the tick is largely believed to be a biological vector of f. tularensis, transmission by mosquitoes and flies is largely believed to be mechanical on the mouthpart through interrupted feedings. however, the mechanism of ... | 2011 | 21687425 |
| toward an understanding of the perpetuation of the agent of tularemia. | the epidemiology of tularemia has influenced, perhaps incorrectly skewed, our views on the ecology of the agent of tularemia. in particular, the central role of lagomorphs needs to be reexamined. diverse observations, some incidental, and some that are more generally reproducible, have not been synthesized so that the critical elements of the perpetuation of francisella tularensis can be identified. developing a quantitative model of the basic reproduction number of f. tularensis may require sep ... | 2011 | 21687803 |
| an elisa to detect serum antibodies to the salivary gland toxin of ixodes holocyclus neumann in dogs and rodents. | the ixodes holocyclus tick causes paralysis in up to 10,000 companion and domestic animals each year in australia. treatment requires the removal of the parasite and the administration of a commercial tick antiserum that is prepared from hyperimmune dogs. each batch of this serum is initially tested for toxin-neutralising potency in a mouse bioassay that is expensive, time consuming, and subjective. with the aim of developing a rapid in vitro assay to replace the bioassay, we used a partially pu ... | 2011 | 21687655 |
| association of different genetic types of francisella-like organisms with the rocky mountain wood tick (dermacentor andersoni) and the american dog tick (dermacentor variabilis) from localities near their northern distributional limits. | dermacentor andersoni and dermacentor variabilis from allopatric and sympatric populations near their northern distributional limits were examined for the presence of francisella species using molecular techniques that targeted 373 bp of the 16s ribosomal rna gene. although there was no evidence for the presence of francisella tularensis in any tick, francisella-like endosymbionts (fles) were common in d. andersoni and d. variabilis adults and immatures. a significantly greater proportion of fem ... | 2011 | 22179251 |
| An insight into the sialotranscriptome and proteome of the coarse bontlegged tick, Hyalomma marginatum rufipes. | Ticks are mites specialized in acquiring blood from vertebrates as their sole source of food and are important disease vectors to humans and animals. Among the specializations required for this peculiar diet, ticks evolved a sophisticated salivary potion that can disarm their host's hemostasis, inflammation, and immune reactions. Previous transcriptome analysis of tick salivary proteins has revealed many new protein families indicative of fast evolution, possibly due to host immune pressure. The ... | 2011 | 21851864 |
| Dermacentor andersoni transmission of Francisella tularensis subsp. novicida reflects bacterial colonization, dissemination, and replication coordinated with tick feeding. | Ticks serve as biological vectors for a wide variety of bacterial pathogens which must be able to efficiently colonize specific tick tissues prior to transmission. The bacterial determinants of tick colonization are largely unknown, a knowledge gap attributed in large part to the paucity of tools to genetically manipulate these pathogens. In this study, we demonstrated that Francisella tularensis subsp. novicida, for which a complete two-allele transposon mutant library has been constructed, ini ... | 2011 | 21930762 |
| assessment of bacterial diversity in the cattle tick rhipicephalus (boophilus) microplus through tag-encoded pyrosequencing. | ticks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. the cattle tick, rhipicephalus (boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. tick microbiomes remain largely unexplored. the objective of this study was to explore the r. microplus microbiome by applying the bacterial 16s tag-encoded flx-titanium amplicon pyrosequencing (btefap) technique to characterize its bacterial ... | 2011 | 21211038 |
| short report: new spotted fever group rickettsia in a rhipicephalus turanicus tick removed from a child in eastern sicily, italy. | a new genotype of spotted fever group rickettsia (sfgr) was identified in rhipicephalus turanicus from eastern sicily. on the basis of current molecular criteria, the genetic characteristics obtained from multiple locus sequence typing satisfy the requirements for candidatus status of this sfgr. further detection and identification of this sfgr during entomological and clinical surveys will be required to establish the prevalence of this rickettsia and its potential pathogenicity for humans. | 2011 | 21212209 |
| isolation of a rickettsial pathogen from a non-hematophagous arthropod. | rickettsial diversity is intriguing in that some species are transmissible to vertebrates, while others appear exclusive to invertebrate hosts. of particular interest is rickettsia felis, identifiable in both stored product insect pests and hematophagous disease vectors. to understand rickettsial survival tactics in, and probable movement between, both insect systems will explicate the determinants of rickettsial pathogenicity. towards this objective, a population of liposcelis bostrychophila, c ... | 2011 | 21283549 |
| complementation of rickettsia rickettsii rela/spot restores a nonlytic plaque phenotype. | spotted fever group rickettsiae are known to produce distinct plaque phenotypes. strains that cause lytic infections in cell culture form clear plaques, while nonlytic strains form opaque plaques in which the cells remain intact. clear plaques have historically been associated with more-virulent species or strains of spotted fever group rickettsiae. we have selected spontaneous mutant pairs from two independent strains of rickettsia rickettsii, the virulent r strain and the avirulent iowa strain ... | 2011 | 21300770 |
| novel genotypes of anaplasma bovis, "candidatus midichloria" sp. and ignatzschineria sp. in the rocky mountain wood tick, dermacentor andersoni. | bovine anaplasmosis, caused by anaplasma marginale, is a vector-borne disease that is enzootic in many parts of the usa. although dermacentor andersoni, a major vector of a. marginale, occurs in canada, the canadian cattle herds are currently considered free of bovine anaplasmosis. there have been two outbreaks of the disease in the province of saskatchewan, but these have been linked to the importation of infected animals. however, the distribution of bovine anaplasmosis may alter with range ex ... | 2011 | 21334146 |
| neural control of salivary glands in ixodid ticks. | studies of tick salivary glands (sgs) and their components have produced a number of interesting discoveries over the last four decades. however, the precise neural and physiological mechanisms controlling sg secretion remain enigmatic. major studies of sg control have identified and characterized many pharmacological and biological compounds that activate salivary secretion, including dopamine (da), octopamine, γ-aminobutyric acid (gaba), ergot alkaloids, pilocarpine (pc), and their pharmacolog ... | 2011 | 22119563 |
| a 60-year meta-analysis of tick paralysis in the united states: a predictable, preventable, and often misdiagnosed poisoning. | tick paralysis (tp) is a neurotoxic poisoning primarily afflicting young girls in endemic regions. recent case series of tp have described increasing misdiagnoses of tp as the guillain-barré syndrome (gbs). a meta-analysis of the scientific literature was conducted using internet search engines to assess the evolving epidemiology of tp. fifty well-documented cases of tp were analyzed over the period 1946-2006. cases were stratified by demographics, clinical manifestations, and outcomes. misdiagn ... | 2010 | 20186584 |
| differences in prevalence of borrelia burgdorferi and anaplasma spp. infection among host-seeking dermacentor occidentalis, ixodes pacificus, and ornithodoros coriaceus ticks in northwestern california. | previous studies revealed that the pacific coast tick (dermacentor occidentalis) is infected occasionally with the agents of lyme disease (borrelia burgdorferi) or human granulocytic anaplasmosis (anaplasma phagocytophilum) and that it is an inefficient experimental vector of b. burgdorferi. the relationship of the pajahuello tick (ornithodoros coriaceus) to each of these bacterial zoonotic agents has not been reported. the primary bridging vector of both bacterial zoonotic agents to humans is t ... | 2010 | 21359090 |
| an update on bovine anaplasmosis (anaplasma marginale) in canada. | 2010 | 21037882 | |
| rhipicephalus microplus salivary gland molecules induce differential cd86 expression in murine macrophages. | abstract: | 2010 | 21054882 |
| stability and tick transmission phenotype of gfp-transformed anaplasma marginale through a complete in vivo infection cycle. | we tested the stability and tick transmission phenotype of transformed anaplasma marginale through a complete in vivo infection cycle. similar to the wild type, the gfp-transformed a. marginale strain established infection in cattle, a natural reservoir host, and persisted in immune competent animals. the tick infection rates for the transformed a. marginale and the wild type were the same. however, there were significantly lower levels of the transformed a. marginale than of the wild type in th ... | 2010 | 21057014 |
| identification of anaplasma marginale outer membrane protein antigens conserved between a. marginale sensu stricto strains and the live a. marginale subsp. centrale vaccine. | live vaccination with anaplasma marginale subsp. centrale (synonym for anaplasma centrale) induces protection against severe disease upon challenge with a. marginale sensu stricto strains. despite over a century of field use, the targets of protective immunity remained unknown. using a broad proteomic approach, we identified the proteins in a challenge sensu stricto strain that were bound by the relevant antibody isotype induced by live vaccination with anaplasma marginale subsp. centrale. a cor ... | 2010 | 21189322 |
| francisella acid phosphatases inactivate the nadph oxidase in human phagocytes. | francisella tularensis contains four putative acid phosphatases that are conserved in francisella novicida. an f. novicida quadruple mutant (acpa, acpb, acpc, and hap [deltaabch]) is unable to escape the phagosome or survive in macrophages and is attenuated in the mouse model. we explored whether reduced survival of the deltaabch mutant within phagocytes is related to the oxidative response by human neutrophils and macrophages. f. novicida and f. tularensis subspecies failed to stimulate reactiv ... | 2010 | 20348422 |
| tick paralysis caused by dermacentor andersoni (acari: ixodidae) is a heritable trait. | the heritability of the ability to cause paralysis was examined in crosses of virulent and avirulent dermacentor andersoni (stiles) (acari: ixodidae). virulence was assessed using hamster bioassay. paralysis was caused by the virulent parental strain but not by the avirulent parental strain. four crosses were made: avirulent female x avirulent male (aa cross), virulent female x virulent male (vv cross), avirulent female x virulent male (av cross), and virulent female x avirulent male (va cross). ... | 2010 | 20380302 |
| coxiella burnetii in humans and ticks in rural senegal. | q fever is a worldwide zoonotic disease caused by coxiella burnetii. epidemiologically, animals are considered reservoirs and humans incidental hosts. | 2010 | 20386603 |
| identification of anaplasma marginale proteins specifically upregulated during colonization of the tick vector. | the transition between infection of the mammalian host and colonization of an arthropod vector is required for the ongoing transmission of a broad array of pathogens, from viruses to protozoa. understanding how this transition is mediated provides opportunities to disrupt transmission through either chemotherapy or immunization. we used an unbiased proteomic screen to identify anaplasma marginale proteins specifically upregulated in the tick compared to the mammalian host. comparative mass spect ... | 2010 | 20439479 |
| persistence of buggy creek virus (togaviridae, alphavirus) for two years in unfed swallow bugs (hemiptera: cimicidae: oeciacus vicarius). | alphaviruses (togaviridae) have rarely been found to persist for long in the adult insects that serve as their vectors. the ectoparasitic swallow bug (hemiptera: cimicidae: oeciacus vicarius horvath), the vector for buggy creek virus (bcrv; togaviridae, alphavirus), lives year-round in the mud nests of its host, the cliff swallow (petrochelidon pyrrhonota vieillot). we measured the prevalence of bcrv in swallow bugs at sites with cliff swallows present and at the same sites after cliff swallows ... | 2010 | 20496591 |
| the expression of genes coding for distinct types of glycine-rich proteins varies according to the biology of three metastriate ticks, rhipicephalus (boophilus) microplus, rhipicephalus sanguineus and amblyomma cajennense. | ticks secrete a cement cone composed of many salivary proteins, some of which are rich in the amino acid glycine in order to attach to their hosts' skin. glycine-rich proteins (grps) are a large family of heterogeneous proteins that have different functions and features; noteworthy are their adhesive and tensile characteristics. these properties may be essential for successful attachment of the metastriate ticks to the host and the prolonged feeding necessary for engorgement. in this work, we an ... | 2010 | 20529354 |
| an insight into the sialotranscriptome of the brown dog tick, rhipicephalus sanguineus. | rhipicephalus sanguineus, known as the brown dog tick, is a common ectoparasite of domestic dogs and can be found worldwide. r.sanguineus is recognized as the primary vector of the etiological agent of canine monocytic ehrlichiosis and canine babesiosis. here we present the first description of a r. sanguineus salivary gland transcriptome by the production and analysis of 2,034 expressed sequence tags (est) from two cdna libraries, one consctructed using mrna from dissected salivary glands from ... | 2010 | 20650005 |
| anaplasma marginale infection with persistent high-load bacteremia induces a dysfunctional memory cd4+ t lymphocyte response but sustained high igg titers. | control of blood-borne infections is dependent on antigen-specific effector and memory t cells and high-affinity igg responses. in chronic infections characterized by a high antigen load, it has been shown that antigen-specific t and b cells are vulnerable to downregulation and apoptosis. anaplasma marginale is a persistent infection of cattle characterized by acute and chronic high-load bacteremia. we previously showed that cd4(+) t cells primed by immunization with an a. marginale outer membra ... | 2010 | 20943884 |
| evaluation of rickettsia japonica pathogenesis and reservoir potential in dogs by experimental inoculation and epidemiologic survey. | rickettsia japonica pathogenesis and reservoir potential in dogs were evaluated by both experimental inoculation and epidemiologic survey. in the experimental inoculation study, dogs 1 and 2 were pretreated with an immunosuppressive dose of cyclosporine 14 days before inoculation and became ill after exposure to r. japonica. dogs exhibited clinical signs, including fever, anorexia, depression, and decreased water consumption, between 36 and 96 h after inoculation, but these signs disappeared spo ... | 2010 | 20980481 |
| analysis of rickettsia typhi-infected and uninfected cat flea (ctenocephalides felis) midgut cdna libraries: deciphering molecular pathways involved in host response to r. typhi infection. | murine typhus is a flea-borne febrile illness that is caused by the obligate intracellular bacterium, rickettsia typhi. the cat flea, ctenocephalides felis, acquires r. typhi by imbibing a bloodmeal from a rickettsemic vertebrate host. to explore which transcripts are expressed in the midgut in response to challenge with r. typhi, cdna libraries of r. typhi-infected and uninfected midguts of c. felis were constructed. in this study, we examined midgut transcript levels for select c. felis serine ... | 2010 | 20017753 |
| wide dispersal and possible multiple origins of low-copy-number plasmids in rickettsia species associated with blood-feeding arthropods. | plasmids are mobile genetic elements of bacteria that can impart important adaptive traits, such as increased virulence or antibiotic resistance. we report the existence of plasmids in rickettsia (rickettsiales; rickettsiaceae) species, including rickettsia akari, "candidatus rickettsia amblyommii," r. bellii, r. rhipicephali, and reis, the rickettsial endosymbiont of ixodes scapularis. all of the rickettsiae were isolated from humans or north and south american ticks. r. parkeri isolates from b ... | 2010 | 20097813 |
| rickettsial ompb promoter regulated expression of gfpuv in transformed rickettsia montanensis. | rickettsia spp. (rickettsiales: rickettsiaceae) are gram-negative, obligate intracellular, alpha-proteobacteria that have historically been associated with blood-feeding arthropods. certain species cause typhus and spotted fevers in humans, but others are of uncertain pathogenicity or may be strict arthropod endosymbionts. genetic manipulation of rickettsiae should facilitate a better understanding of their interactions with hosts. | 2010 | 20126457 |
| extraction of total nucleic acids from ticks for the detection of bacterial and viral pathogens. | ticks harbor numerous bacterial, protozoal, and viral pathogens that can cause serious infections in humans and domestic animals. active surveillance of the tick vector can provide insight into the frequency and distribution of important pathogens in the environment. nucleic-acid based detection of tick-borne bacterial, protozoan, and viral pathogens requires the extraction of both dna and rna (total nucleic acids) from ticks. traditional methods for nucleic acid extraction are limited to extrac ... | 2010 | 20180313 |
| detection of a new arsenophonus-type bacterium in canadian populations of the rocky mountain wood tick, dermacentor andersoni. | ticks of the genus dermacentor are important vectors of human and animal pathogens in north america. they also carry a variety of endosymbiotic (i.e. non-pathogenic) bacteria. the american dog tick, d. variabilis, is known to be infected with gammaproteobacteria of the genus arsenophonus. however, there have been no previous reports of arsenophonus-type bacteria in the rocky mountain wood tick, d. andersoni, a species that is sympatric with d. variabilis in the western parts of its distributiona ... | 2010 | 20186465 |
| detection of "candidatus rickettsia sp. strain argentina"and rickettsia bellii in amblyomma ticks (acari: ixodidae) from northern argentina. | ixodid ticks were collected from vegetation and from humans, wild and domestic mammals in a rural area in the semi-arid argentine chaco in late spring 2006 to evaluate their potential role as vectors of spotted fever group (sfg) rickettsiae. a total of 233 adult ticks, identified as amblyomma parvum, amblyomma tigrinum and amblyomma pseudoconcolor, was examined for rickettsia spp. we identified an sfg rickettsia of unknown pathogenicity, "candidatus rickettsia sp. strain argentina", in a. parvum ... | 2010 | 20186466 |
| bartonella spp. transmission by ticks not established. | bartonella spp. infect humans and many animal species. mainly because pcr studies have demonstrated bartonella dna in ticks, some healthcare providers believe that these microorganisms are transmitted by ticks. b. henselae, in particular, is regarded as being present in and transmissible by the ixodes scapularis tick. the presence of a microbial agent within a tick, however, does not imply that the tick might transmit it during the course of blood feeding and does not confer epidemiologic import ... | 2010 | 20202410 |
| isolation of rickettsia parkeri and identification of a novel spotted fever group rickettsia sp. from gulf coast ticks (amblyomma maculatum) in the united states. | until recently, amblyomma maculatum (the gulf coast tick) had garnered little attention compared to other species of human-biting ticks in the united states. a. maculatum is now recognized as the principal vector of rickettsia parkeri, a pathogenic spotted fever group rickettsia (sfgr) that causes an eschar-associated illness in humans that resembles rocky mountain spotted fever. a novel sfgr, distinct from other recognized rickettsia spp., has also been detected recently in a. maculatum specime ... | 2010 | 20208020 |
| association of pathogen strain-specific gene transcription and transmission efficiency phenotype of anaplasma marginale. | efficient transmission of pathogens by an arthropod vector is influenced by the ability of the pathogen to replicate and develop infectiousness within the arthropod host. while the basic life cycle of development within and transmission from the arthropod vector are known for many bacterial and protozoan pathogens, the determinants of transmission efficiency are largely unknown and represent a significant gap in our knowledge. the st. maries strain of anaplasma marginale is a high-transmission-e ... | 2010 | 20308303 |
| expression of heat shock and other stress response proteins in ticks and cultured tick cells in response to anaplasma spp. infection and heat shock. | ticks are ectoparasites of animals and humans that serve as vectors of anaplasma and other pathogens that affect humans and animals worldwide. ticks and the pathogens that they transmit have coevolved molecular interactions involving genetic traits of both the tick and the pathogen that mediate their development and survival. in this paper, the expression of heat shock proteins (hsps) and other stress response proteins (srps) was characterized in ticks and cultured tick cells by proteomics and t ... | 2010 | 22084679 |