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age grading anopheles arabiensis: their gorging and surviving responses using a membrane feeding system.literature on artificial membrane feeding has traditionally supported the notion that mosquitoes have to be enough old to present a high gorging rate and enough young to present a high surviving rate. in order to know the best mosquito age to perform a plasmodium sp. experimental infection revealed by oocyst examination, anopheles arabiensis of known ages were fed on baudruche membrane with fresh human blood thermostated at 37 degrees c. the response was evaluated in term of gorging after ten mi ...19989754303
sequence variation in the small-subunit rrna gene of plasmodium malariae and prevalence of isolates with the variant sequence in sichuan, china.by two pcr-based diagnostic methods, plasmodium malariae infections have been rediscovered at two foci in the sichuan province of china, a region where no cases of p. malariae have been officially reported for the last 2 decades. in addition, a variant form of p. malariae which has a deletion of 19 bp and seven substitutions of base pairs in the target sequence of the small-subunit (ssu) rrna gene was detected with high frequency. alignment analysis of plasmodium sp. ssu rrna gene sequences reve ...19989774600
[control of blood transfusion malaria in an endemic and in a non-endemic region in brazil].the aim of the present work was to establish appropriate criteria for screening of donor blood from regions with distinct malaria epidemiological characteristics. three locations with different screening criteria were studied: são paulo, sp (with no vectorial transmission), belém, pa (with low active transmission) and matupá and peixoto de azevedo, mt (with high active transmission). the malaria parasite--plasmodium sp--was searched for by "thick film", qbc test and antigen immunofluorescence te ...19989477695
acquired immune responses to the n- and c-terminal regions of plasmodium vivax merozoite surface protein 1 in individuals exposed to malaria.in this study, we evaluated the naturally acquired immune response to plasmodium vivax merozoite surface protein 1 (pvmsp1) in individuals with recent clinical episodes of malaria from the state of para, brazil. ten recombinant proteins representing the first 682 amino acids (aa) of the n-terminal region and one representing the final 111 aa of the c-terminal region were expressed in escherichia coli as glutathione s-transferase fusion proteins. both of these regions have been suggested as candi ...19979125537
parasitic infections in sickle cell crisis: nigerian experience.data collected on 150 sickle cell patients in nigeria were analyzed to determine the frequency of parasitic infections in clinical and hematologic crisis. fifty-three adult and 97 pediatric patients (mean age: 27.6 years and 9.7 years, respectively) were studied. of these patients, 82 were males and 68 females. one hundred thirty-nine had the ss and 11 the sc genotype. blood samples collected from patients on admission for sickle cell-related illnesses were examined microscopically for evidence ...19979145635
autologous lymphoblastoid cell lines stably transfected with plasmodium falciparum circumsporozoite protein as targets in cytotoxic t-lymphocyte assays.to produce cell lines that can be used as a continuous source of antigen presenting cells for stimulating t-cell lines and clones and as targets in cytotoxic t-lymphocyte (ctl) assays, we used a retroviral vector with a simian virus (sv40) early promotor to transfer a plasmodium falciparum circumporozoite (pfcsp) gene into human ebv transformed b-lymphoblastoid cell lines (b-lcl). we herein report successful, stable transfection and cell surface expression of this gene, as confirmed by pcr, west ...19979161886
malaria in a captive emu (dromaius novaehollandiae) from florida.severe anemia and intraerythrocytic stages of an unusual plasmodium sp. were identified in the blood of a 5-month-old female emu (dromaius novaehollandiae) that had a 4-week history of malaise. examination of blood taken 3 days prior to the death of the bird revealed a packed cell volume of 10%. erythrocytes showed a marked regenerative response. approximately 4% of erythrocytes had intracytoplasmic inclusions, mainly within immature cells, resembling malarial parasites. the schizonts were a com ...19968790903
the mic1 microneme protein of toxoplasma gondii contains a duplicated receptor-like domain and binds to host cell surface.the cdna encoding the toxoplasma gondii microneme protein mic1 and the corresponding gene have been cloned and sequenced. the mic1 gene contains three introns. the cdna encodes a 456 amino acid (aa) sequence, with a typical signal sequence and no other trans-membrane domain. the protein contains a tandemly duplicated domain with conservation of cysteines and presents distant homology with the plasmodium sp. microneme protein trap-ssp2. the mic1 protein from tachyzoite lysates and a pmal recombin ...19969027753
hemosporid (apicomplexa, hematozoea, hemosporida) community structure off pattern in wintering wild turkeys.the hemosporid community of 76 wild turkeys (meleagris gallopavo silvestris) from south carolina (usa) was examined using thin blood smears collected during january and february 1994. high prevalences and low abundances of hemosporids characterized this community. leucocytozoon smithi and haemoproteus meleagridis occurred in 100% and 54% of the turkeys, respectively; a plasmodium sp. was found in one bird. prevalence of h. meleagridis was significantly higher in juvenile turkeys than adults, but ...19958592366
a pcr test for avian malaria in hawaiian birds.the decline of native hawaiian forest birds since european contact is attributed to factors ranging from habitat destruction to interactions with introduced species. remaining populations of hawaiian honeycreepers (fringillidae: drepanidinae) are most abundant and diverse in high elevation refuges above the normal range of disease-carrying mosquitoes. challenge experiments suggest that honeycreepers are highly susceptible to avian malaria (plasmodium sp.) but resistance exists in some species. i ...19958564006
hemosporid parasitism of mallards at the southern periphery of their breeding range.thin blood smears from 37 juvenile mallards (anas platyrhynchos) collected in the playa lakes region of texas during summers 1990 and 1991 were examined for hemosporids. only one bird was infected with a plasmodium sp. parasite intensity was seven per 10,000 erthrocytes. the low prevalence of plasmodium sp. and the lack of other blood parasites in resident mallards suggests that hemosporid parasitism at the southern periphery of their breeding range is very different from that at the northern br ...19947702525
cytokines and t-cell response in malaria.the intracellular protozoan plasmodium sp induces a complex immune response which sometimes implies serious pathological effects for the host. according to in vitro studies and epidemiological surveys, several effector mechanisms are displayed against plasmodial blood stages and a large interaction between humoral and cell-mediated immunity is presumed to occur among protected individuals. the key role of t cells in the antiplasmodial immune response is now well established, but all the regulato ...19947919100
blood parasites in birds from monteverde, costa rica.in a survey of avian blood parasites in costa rica, 51 (11%) of 479 birds sampled were infected by at least one species of hematozoan. fourteen of the 60 species of birds in the survey were examined for the first time. infections were most common in ramphastids and emberizids and infrequent in other taxa. among resident species, infections were more commonly detected during the wet season when most birds breed than during the dry season when few birds breed. infections caused by haemoproteus sp. ...19938258853
stage-specific ultrastructural effects of desferrioxamine on plasmodium falciparum in vitro.desferrioxamine (dfo) is an iron chelator that inhibits the in vitro and in vivo growth of rodent and human malarial parasites. previous studies with this chelator have suggested that it might interfere with the intraerythrocytic growth of plasmodium sp. by withholding iron from any of several essential iron-dependent parasite enzymes, including those involved in co2 fixation, mitochondrial electron transport, pyrimidine synthesis, and the reduction of ribonucleotides for dna synthesis. we studi ...19911951869
abnormalities of leukocyte histograms resulting from microorganisms.the authors report a series of 13 patients seen in their laboratory during october 1985 to august 1988 in which the presence of bacterial, fungal, or malarial parasites visible on peripheral smear was correlated with an abnormal leukocyte histogram. samples submitted for complete blood count and differential counts were analyzed with coulter s-plus vi (seven specimens) or s-plus stkr (six specimens) instrumentation. organisms visualized on the wright-stained peripheral smears included histoplasm ...19902321584
[plasmodia of lemurs in madagascar].lemur macaco macaco from ambanja region was found polyparasitized by four different species of plasmodium: --plasmodium coulangesi recently described by lepers et al. (1989). --p. bucki n. sp.: its main differential characterisitics are the large numvber (32) of merozoites produced in mature schizonts and the stippling, resembling maurer's dots, in an hypertrophied pinkish erythrocyte. --p. percygarnhami n. sp. (= p. girardi sensu uilenberg, 1970 pro parte and sensu garnham et uilenberg, 1975 pr ...19892764452
[a new plasmodium species from a lemur: plasmodium coulangesi n. sp].the splenectomy of a female lemur macaco macaco, showing a scanty blood infection by haemosporidians and a trypanosome was followed by a dramatic increase of the rate of intra-erythrocytic parasitaemia. three species of plasmodium where then identified. the first species was unknown; it is described in this paper and named plasmodium coulangesi n. sp. it is characterised by--the low (6). constant number of merozoites in mature schizonts,--the disposition of the pigment, well apart from the paras ...19892764451
pneumocystis carinii: taxing taxonomy.the detailed taxonomy of pneumocystis carinii has not been decided. most experts agree that it is either a fungus or a protozoan. in favor of a fungal designation are the recent reports that 16s and 18s rrna sequences of p. carinii have greater homology to saccharomyces cerevisiae and neurospora crassa than to selected protozoa. other studies show that sequences of the 18s and 26s rrna, microtubular ultrastructure, membrane fusion potential and dna content per cell resemble protozoa more than fu ...19892477272
neutral proteases involved in the reinvasion of erythrocytes by plasmodium merozoites.neutral proteases of plasmodium sp erythrocytic stages were studied by means of a sensitive fluorogenic method and gelatin-sds-page. the substrates gluconoyl-val-leu-gly-lys(or arg)-3-amido-9-ethylcarbazole were selectively hydrolyzed by an endopeptidase from rodent plasmodium berghei (pb) and plasmodium chabaudi (pc) and from human plasmodium falciparum (pf) parasites. these endopeptidases were purified from 100,000-g soluble schizont extract by high pressure liquid chromatography; they have a ...19883067801
selected epidemiological and clinical aspects of malaria in the material of the institute of maritime and tropical medicine in gdynia.despite numerous endeavours to eradicate malaria remains a problem to be solved. this is reflected in high morbidity and mortality rates in endemic zones of this disease, and in the continually growing parasites resistance to the drugs applied within chemoprophylaxis and the treatment employed. in the compass of 1976-1987 a group of 20 thousand people, back from the tropics, was examined. in that number 415 having suffered from malaria while stay in the endemic zone of the disease, in 64 patient ...19883077994
hematozoan parasites of rio grande wild turkeys from southern texas.one hundred twenty-three of 300 blood samples (41%) taken from rio grande wild turkeys (meleagris gallopavo intermedia) from three locations in southern texas (welder wildlife refuge, chaparrosa ranch, and campo alegre ranch) and subinoculated into domestic broad-breasted white turkey poults were positive for a plasmodium (novyella) sp. analysis of blood films from 350 turkeys revealed haemoproteus meleagridis in 76% of the birds. a significantly greater mean parasite intensity was observed in b ...19883127605
plasmodium relictum as a cause of avian malaria in wild-caught magellanic penguins (spheniscus magellanicus).avian malaria (plasmodium relictum) caused significant mortality in wild-caught magellanic penguins (spheniscus magellanicus) in 1986 at the blank park zoo in des moines, iowa (usa). in early winter, wild birds were captured off the southern coast of chile and flown to detroit, michigan for a 38 day quarantine. after quarantine, 18 birds were dispersed to lansing, michigan, six to a facility in maine, and 46 to des moines, iowa. upon arrival in des moines, several penguins became weak and inacti ...19883193555
[attempted immunodepression of a lemur macaco macaco carrying plasmodium sp. utilization of corticoids]. 19883242426
observations on malaria in european perching birds (passeriformes).893 passeriformes birds belonging to 44 species and 14 families were investigated for blood parasites during a study running over 5 years. more than 1700 blood samples were taken. an average of 14% of the birds proved to be infected, most frequently with leucozytozoon and haemoproteus ( parahaemoproteus ). plasmodium sp. as well as other blood parasites were rarely found. positive findings were correlated to the number of birds investigated per species, to the frequency and mode of inspections, ...19846428078
an outbreak of malaria in penguins at the farm-land zoo.in june of 1983 authors had investigated the occurrence of the avian malaria in the humboldt penguins imported from japan to the farm-land zoo in central district of korea. the disease was characterized by acute course and high mortality. the peripheral blood smears from the affected penguins demonstrated different developmental stages of plasmodium sp. in the mature erythrocytes. the predominant gross lesions noticed were pulmonary and epicardial edema and hepato-splenomegaly. microscopically t ...198412891023
parasitic infections of the grey-breasted helmet guinea-fowl (numida meleagris galeata) in nigeria.the major helminth parasites found in wild, semi-wild and golden sovereign stock guinea fowl were heterakis gallinarum, ascaridia galli, capillaria caudinflata, raillietina tetragona and r. echinobothrida, while eimeria species was the most important gastro-intestinal protozoan parasite. the incidence of the latter was higher in the semi-wild stock than in the wild stock. necropsy of dead guinea-fowl indicated that a. galli, h. gallinarum and eimeria species were indeed responsible for their dea ...19836683038
comparative study of amino acid production in erythrocytes parasitized by plasmodium sp. and babesia hylomysci.parasitized red blood cells are incubated in an isotonic buffer and the release of amino acids is determined in the cells and the buffer according to time. erythrocytes parasitized by p. yoelii nigeriensis showed an intense release of amino acids whereas those parasitized by b. hylomysci behaved almost like the uninfected erythrocytes. a large amount of the amino acids produced by proteolysis are thus released out of the red blood cell. red blood cells parasitized by p. berghei and p. berghei ch ...19836872523
blood parasites of wild and domestic animals from south kalimantan (borneo), indonesia.wild and domestic animals trapped from forests, villages, and rice fields in south kalimantan (3 degrees 20' s, 115 degrees 02' e, 25 m) were examined for blood parasites using giemsa stained thick and thin blood films and nuclepore filter preparations of peripheral vein and heart puncture blood. presbytis cristatus (silvered leaf monkey) (25%) and felis catus (domestic cat) (7%) were infected with brugia malayi and b. pahangi. in addition, p. cristatus was infected with wuchereria kalimantani ( ...19816789456
complement does not facilitate plasmodial infections.the influence of the complement (c) system on plasmodial infections in vivo (plasmodium berghei in rats) and in vitro (plasmodium falciparum) has been determined. in rats c3 depletion by treatment of animals with the c3 inactivator isolated from cobra venom factor results in infection that develops more rapidly, reaches a higher peak of parasitemia and is associated with an increased mortality rate (60%), in contrast to a lower degree of parasitemia and lack of mortality in c3-intact rats. the i ...19817012239
resistance to babesia spp. and plasmodium sp. in mice pretreated with an extract of coxiella burnetii.mice injected intravenously with a commercially available extract of coxiella burnetii prepared for use as the antigen in the complement fixation diagnostic test for q fever were subsequently resistant to infection with babesia microti, babesia rodhaini, and plasmodium vinckei petteri. the parasites appeared to die inside circulating erythrocytes. protection was unaffected by exposing the pretreated mice to 900 rads on the day before they were infected. to explain these findings, it is postulate ...1979378850
infection and mortality in captive wild-trapped canvasback ducks.fungal, bacterial and malarial infections, as well as malnutrition caused heavy mortality in a group of wild-trapped canvasbacks (aythya valisineria) held in 10 x 3 x 2 m open-water pens. deaths occurred between 21 and 158 days after confinement and were associated with infections of aspergillus fumigatus, escherichia coli, staphylococcus sp., streptococcus sp., klebsiella sp., enterobacter sp., and plasmodium sp. infection and mortality was believed to result from reduced resistance associated ...19761255909
hemoprotozoa in mourning doves and other small birds of western oklahoma.blood smears obtained from 370 birds live-trapped in western oklahoma were examined for hemoprotozoa. haemoproteus spp. were found in 189 (90.4%) mourning doves (zenaida macroura), one oriole (icterus galbula), two mockingbirds (mimus polyglottos), and three brown thrashers (toxostoma rufum). plasmodium sp. was present in one brown thrasher. haemoproteus spp. in the mourning dove were identified as h. sacharovi and h. maccallumi, with the latter species predominating. the average parasitemia for ...1975811816
a plasmodium sp. from the crested agamid lizard (gonyocephalus grandis) in peninsular malaysia. 19744432107
plasmodium sp infecting turkeys in northern nigeria. 19744450382
plasmodium sp.: topography of intra- and extracellular parasites. 19734130524
plasmodium sp.: the relationship between erythrocyte morphology and parasitization in chickens, rats, and mice. 19725010603
resistance of ducks to a plasmodium sp. induced by a filterable agent. 19694987047
a plasmodium sp. from a glossy tree-starling (aplonis panayensis strigatus). 196514306412
sporozoites of plasmodium sp. in anopheles mangyanus and oocysts in a. kochi. 194818915970
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