Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| nonsuppurative meningoencephalitis associated with aleutian mink disease parvovirus infection in ranch mink. | severe nonsuppurative meningoencephalitis associated with aleutian mink disease parvovirus (adv) infection was observed in adult ranch mink. brain lesions included severe, locally extensive to coalescing lymphoplasmacytic meningoencephalitis with accompanying gliosis, satellitosis, and mild extension of inflammation into the leptomeninges. adv was identified in mesenteric lymph node, spleen, brain, and liver of affected mink by polymerase chain reaction techniques. sequences of the adv isolate ( ... | 2000 | 10730948 |
| identification of a cell surface protein from crandell feline kidney cells that specifically binds aleutian mink disease parvovirus. | aleutian mink disease parvovirus (adv) is the etiological agent of aleutian disease of mink. the acute disease caused by adv consists of permissive infection of alveolar type ii cells that results in interstitial pneumonitis. the permissive infection is experimentally modeled in vitro by infecting crandell feline kidney (crfk) cells with a tissue culture-adapted isolate of adv, adv-g. adv-g vp2 empty virions expressed in a recombinant baculovirus system were analyzed for the ability to bind to t ... | 1999 | 10196278 |
| epitope mapping of aleutian mink disease parvovirus virion protein vp1 and 2. | six overlapping fragments of the aleutian mink disease parvovirus (amdv) virion protein vp1 and 2 (vp1/2) gene were inserted into the expression vector pmal-c2. four of the clones carried large overlapping fragments covering the entire vp1/2 gene. the remaining two clones covered specifically chosen regions within the vp1/2 gene. using a western blotting detection system, sera from amdv-infected mink were tested against the recombinant polypeptides. these studies showed reactions primarily direc ... | 1999 | 10219758 |
| three-dimensional structure of aleutian mink disease parvovirus: implications for disease pathogenicity. | the three-dimensional structure of expressed vp2 capsids of aleutian mink disease parvovirus strain g (advg-vp2) has been determined to 22 a resolution by cryo-electron microscopy and image reconstruction techniques. a structure-based sequence alignment of the vp2 capsid protein of canine parvovirus (cpv) provided a means to construct an atomic model of the advg-vp2 capsid. the advg-vp2 reconstruction reveals a capsid structure with a mean external radius of 128 a and several surface features si ... | 1999 | 10400786 |
| replication of aleutian mink disease parvovirus in vivo is influenced by residues in the vp2 protein. | aleutian mink disease parvovirus (adv) is the etiological agent of aleutian disease of mink. several adv isolates have been identified which vary in the severity of the disease they elicit. the isolate adv-utah replicates to high levels in mink, causing severe aleutian disease that results in death within 6 to 8 weeks, but does not replicate in crandell feline kidney (crfk) cells. in contrast, adv-g replicates in crfk cells but does not replicate in mink. the ability of the virus to replicate in ... | 1999 | 10482625 |
| molecular and structural basis of the evolution of parvovirus tropism. | parvoviruses have small genomes and, consequently, are highly dependent on their host for various functions in their reproduction. since these viruses generally use ubiquitous receptors, restrictions are usually intracellularly regulated. a lack of mitosis, and hence absence of enzymes required for dna replication, is a powerful block of virus infection. allotropic determinants have been identified for several parvoviruses: porcine parvovirus, canine parvovirus (cpv), feline parvovirus (feline p ... | 1999 | 10497831 |
| unusual, high genetic diversity of aleutian mink disease virus. | the genetic diversity of aleutian mink disease virus (amdv) was examined. sequences obtained from 35 clinical samples were compared with five published sequences. an unusual, high genetic variability was revealed. three phylogenetic subgroups of amdv were identified, and the presence of more than one genotype at some farms was detected. | 1999 | 10565948 |
| analyses of leucocytes in blood and lymphoid tissues from mink infected with aleutian mink disease parvovirus (amdv). | mink were infected with aleutian mink disease parvovirus (amdv) and sacrificed at monthly intervals after infection. during this time humoral immune responses and leucocyte numbers in blood, mesenteric lymph node, spleen and thymus were monitored. serum hypergammaglobulinaemia was observed together with elevated antibody responses to amdv ns1 and vp1/2 proteins. in blood, a highly significant increase in cd8+ lymphocytes was observed. however, (presumed)cd4+ cells defined as cd3+cd8- cells, and ... | 1998 | 9656422 |
| vaccination with aleutian mink disease parvovirus (amdv) capsid proteins enhances disease, while vaccination with the major non-structural amdv protein causes partial protection from disease. | vaccination studies were performed with partially purified recombinant amdv vp1/2 capsids as well as with the major amdv non-structural protein (ns1). all vaccine constructs induced an antibody response, but did not prevent infection upon challenge with amdv. the severity of aleutian disease (ad) was judged by the serum gammaglobulin level, the quantity of peripheral blood cd8 lymphocytes, antibody titers to vp1/2 and ns1 proteins and mink death rates. the vp1/2 vaccine constructs enhanced the d ... | 1998 | 9682374 |
| a comparison between permissive and restricted infections with aleutian mink disease parvovirus (adv): characterization of the viral protein composition at nuclear sites of virus replication. | we used three-color fluorescent labeling and confocal microscopy to compare the permissive and the antibody-mediated, restricted replication of aleutian mink disease parvovirus (adv). in both permissive (crfk cells) and restricted (k562 cells) situations, both adv non-structural proteins (ns1 and ns2) concentrated at focal sites in the nucleus, which also contained viral dna. bromodeoxyuridine labeling demonstrated that these sites also supported active adv single-strand dna synthesis, indicatin ... | 1998 | 9784064 |
| construction of pathogenic molecular clones of aleutian mink disease parvovirus that replicate both in vivo and in vitro. | the adv-g isolate of aleutian mink disease parvovirus (adv) replicates permissively in crandell feline kidney (crfk) cells but is nonpathogenic for mink, whereas the highly pathogenic adv-utah isolate is nonviable in crfk cells. to assign control of host range in crfk cells and pathogenicity to specific regions of the adv genome, we constructed a full-length molecular clone chimeric between adv-g and adv-utah. if either the map unit (mu) 54-65 (clone g/u-5) or mu 65-88 (clone g/u-7) sections wer ... | 1998 | 9837793 |
| expression of aleutian mink disease parvovirus capsid proteins in defined segments: localization of immunoreactive sites and neutralizing epitopes to specific regions. | the capsid proteins of the adv-g isolate of aleutian mink disease parvovirus (adv) were expressed in 10 nonoverlapping segments as fusions with maltose-binding protein in pmal-c2 (pvp1, pvp2a through pvp2i). the constructs were designed to capture the vp1 unique sequence and the portions analogous to the four variable surface loops of canine parvovirus (cpv) in individual fragments (pvp2b, pvp2d, pvp2e, and pvp2g, respectively). the panel of fusion proteins was immunoblotted with sera from mink ... | 1997 | 8985402 |
| s-phase-dependent cell cycle disturbances caused by aleutian mink disease parvovirus. | we examined replication of the autonomous parvovirus aleutian mink disease parvovirus (adv) in relation to cell cycle progression of permissive crandell feline kidney (crfk) cells. flow cytometric analysis showed that adv caused a composite, binary pattern of cell cycle arrest. adv-induced cell cycle arrest occurred exclusively in cells containing de novo-synthesized viral nonstructural (ns) proteins. production of adv ns proteins, indicative of adv replication, was triggered during s-phase trav ... | 1997 | 8995664 |
| aleutian mink disease parvovirus infection of k562 cells is antibody-dependent and is mediated via an fc(gamma)rii receptor. | aleutian mink disease parvovirus (adv) infects macrophages but infection is restricted. using the human macrophage cell line u937, we reported infection to be antibody-dependent [22,23]. recently, we learned that the u937 cells were contaminated with the human cell line, k562 [12]. to clarify which cell line actually supported adv infection, we studied the antibody-dependent enhancement (ade) of adv infection in pure lines of u937 and k562. after infection with adv complexed with mink anti-adv a ... | 1997 | 9125049 |
| two parvoviruses that cause different diseases in mink have different transcription patterns: transcription analysis of mink enteritis virus and aleutian mink disease parvovirus in the same cell line. | the two parvoviruses of mink cause very different diseases. mink enteritis virus (mev) is associated with rapid, high-level viral replication and acute disease. in contrast, infection with aleutian mink disease parvovirus (adv) is associated with persistent, low-level viral replication and chronic severe immune dysregulation. in the present report, we have compared viral transcription in synchronized crfk cells infected with either mev or adv using a nonradioactive rnase protection assay. the ov ... | 1997 | 9188563 |
| the relationship between capsid protein (vp2) sequence and pathogenicity of aleutian mink disease parvovirus (adv): a possible role for raccoons in the transmission of adv infections. | aleutian mink disease parvovirus (adv) dna was identified by pcr in samples from mink and raccoons on commercial ranches during an outbreak of aleutian disease (ad). comparison of dna sequences of the hypervariable portion of vp2, the major capsid protein of adv, indicated that both mink and raccoons were infected by a new isolate of adv, designated adv-tr. because the capsid proteins of other parvoviruses play a prominent role in the determination of viral pathogenicity and host range, we decid ... | 1996 | 8551624 |
| investigation of the pathogenesis of transplacental transmission of aleutian mink disease parvovirus in experimentally infected mink. | the transplacental transmission of aleutian mink disease parvovirus (adv) was studied in experimental infection of 1-year-old female non-aleutian mink. the adv-seronegative female mink were inoculated with adv prior to mating or after the expected implantation of the embryos during pregnancy. a group of uninfected females served as a control group. animals from each group were killed prior to or shortly after parturition. the in situ hybridization technique with radiolabeled strand-specific rna ... | 1996 | 8627663 |
| subcellular localization of aleutian mink disease parvovirus proteins and dna during permissive infection of crandell feline kidney cells. | confocal microscopy allowed us to localize viral nonstructural (ns) and capsid (vp) proteins and dna simultaneously in cells permissively infected with aleutian mink disease parvovirus (adv). early after infection, ns proteins colocalized with viral dna to form intranuclear inclusions, whereas vp proteins formed hollow intranuclear shells around the inclusions. later, nuclei had irregular outlines and were virtually free of adv products. in these cells, inclusions of viral dna with or without as ... | 1996 | 8627805 |
| clusters of lymphoma in ferrets. | cluster outbreaks of lymphoma and leukemia have been associated with viral infections in many species including humans, cattle, and cats. this study describes epidemiological, clinical, and pathological features of cluster outbreaks of lymphoma in multiferret households and examines and compares the aleutian disease virus (adv) and feline leukemia virus (felv) status of cases, ferrets at risk, and controls. three ferret groups with 21 cases of histologically diagnosed lymphoma (12.6% cumulative ... | 1996 | 8630683 |
| identification of a dna segment in ferret aleutian disease virus similar to a hypervariable capsid region of mink aleutian disease parvovirus. | a 401bp dna fragment of ferret aleutian disease virus (adv) was amplified using pcr primers spanning a hypervariable region of mink adv capsid sequence. the amplified fragment was 88-89% homologous to the same region of previously known sequence of three different strains of mink adv, however, as low as 54% homology was observed when compared with a 39bp segment known as hypervariable region. within the predicted 13 amino acid hypervariable region, the ferret adv sequence differed at 6 positions ... | 1996 | 8774691 |
| a cluster of cases of juvenile mediastinal lymphoma in a ferret colony. | three cases of juvenile mediastinal lymphoma developed in a laboratory colony of ferrets. two ferrets became acutely moribund, and one was found dead with no preceding signs of illness. splenomegaly, hepatomegaly, and a large thoracic mass were the primary features in each case. all three ferrets had multiorgan metastasis of the tumor. two ferrets were tested for feline leukemia virus and aleutian disease virus with negative results. | 1996 | 8799931 |
| investigation of an outbreak of aleutian disease on a commercial mink ranch. | to determine the modes of transmission of aleutian mink disease in a natural outbreak. | 1996 | 8950422 |
| purification and characterization of the major nonstructural protein (ns-1) of aleutian mink disease parvovirus. | we have previously described the expression of the major nonstructural protein (ns-1) of aleutian mink disease parvovirus (adv) in insect cells by using a baculovirus vector (j. christensen, t. storgaard, b. bloch, s. alexandersen, and b. aasted, j. virol. 67:229-238, 1993). to study its biochemical properties, adv ns-1 was expressed in sf9 insect cells and purified to apparent homogeneity with a combination of nuclear extraction, zn2+ ion chromatography, and immunoaffinity chromatography on mon ... | 1995 | 7853520 |
| aleutian mink disease: puzzles and paradigms. | aleutian mink disease (ad) is a naturally occurring persistent virus infection of mink caused by the aleutian mink disease parvovirus (adv). the classical form of ad, which occurs in adult mink, is notable for high titers of antiviral antibodies, hypergammaglobulinemia, plasmacytosis, and immune complex disease. in addition, there is a progressive renal disease characterized by mesangial proliferative glomerulonephritis and severe interstitial nephritis. development of ad depends on both host an ... | 1994 | 7889316 |
| sequence comparison of the non-structural genes of four different types of aleutian mink disease parvovirus indicates an unusual degree of variability. | the present work shows that at least four different sequence types of aleutian mink disease parvovirus (adv) are present in adv isolates from mink. we here report the nucleotide sequences of these four types of adv from nucleotide 123 to 2208 (map unit 3 to 46). this part of the genome encodes three non-structural (ns) proteins of adv. comparison of the deduced amino acid sequences of these ns proteins showed that the adv proteins are much less conserved than the ns proteins from other members o ... | 1994 | 7998830 |
| expression of aleutian mink disease parvovirus capsid proteins in a baculovirus expression system for potential diagnostic use. | a 2.3-kb cdna clone encoding aleutian mink disease parvovirus (adv) structural proteins vp1 and vp2 was inserted into the polyhedron gene of autographa californica nuclear polyhedrosis virus (acnpv) and expressed by the recombinant virus, acadv-1, in spodoptera frugiperda-9 cells. sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western immunoblot analysis (wia) indicated that synthesis of both vp1 and vp2 was being directed by acadv-1. fluorescence microscopic examination of acadv- ... | 1994 | 8011776 |
| role of alveolar type ii cells and of surfactant-associated protein c mrna levels in the pathogenesis of respiratory distress in mink kits infected with aleutian mink disease parvovirus. | neonatal mink kits infected with aleutian mink disease parvovirus (adv) develop an acute interstitial pneumonia with clinical symptoms and pathological lesions that resemble those seen in preterm human infants with respiratory distress syndrome and in human adults with adult respiratory distress syndrome. we have previously suggested that adv replicates in the alveolar type ii epithelial cells of the lung. by using double in situ hybridization, with the simultaneous use of a probe to detect adv ... | 1994 | 8139047 |
| acute interstitial pneumonia in mink kits inoculated with defined isolates of aleutian mink disease parvovirus. | the present study addressed the causal role of aleutian mink disease parvovirus (adv) in acute interstitial pneumonia in mink kits. all the examined isolates of adv caused interstitial pneumonia in newborn kits, although the severity of disease and the mortality varied. these findings indicate that adv is the direct causal agent of this disease in mink kits and that cofactors, which could have been present in the original adv-k isolate, do not play a role. acute interstitial pneumonia characteri ... | 1994 | 8203085 |
| interstitial nephritis in aleutian mink disease. possible role of cell-mediated immunity against virus-infected tubular epithelial cells. | aleutian mink disease (ad) has been characterized by immune complex glomerulonephritis associated with persistent infection of aleutian mink disease parvovirus (adv). histopathological examination of kidneys from adv-infected mink in this study revealed that interstitial nephritis characterized by prominent damage of renal tubuli and lymphocyte infiltration was also common in ad along with glomerulonephritis. by using strand-specific in situ molecular hybridization technique, replication of adv ... | 1994 | 8203470 |
| pathogenesis of aleutian mink disease parvovirus infection: effects of suppression of antibody response on viral mrna levels and on development of acute disease. | we suppressed the b-cell development and antibody response in mink by using treatment with polyclonal anti-immunoglobulin m (anti-igm) to study the effects of antiviral antibodies on development of aleutian mink disease parvovirus (adv)-induced disease in more detail. newborn mink kits were injected intraperitoneally with 1 mg of either anti-igm or a control preparation three times a week for 30 to 34 days. at 21 days after birth, groups of mink kits were infected with the highly virulent united ... | 1994 | 8289377 |
| expression of aleutian mink disease parvovirus proteins in a baculovirus vector system. | we have previously published a detailed transcription map of aleutian mink disease parvovirus (adv) and proposed a model for the translation of the two virion structural proteins (vp1 and vp2) and three nonstructural proteins (ns-1, ns-2, and ns-3) (s. alexandersen, m. e. bloom, and s. perryman, j. virol. 62:3684-3994, 1988). to verify and further characterize this model, we cloned the predicted open reading frames for ns-1, ns-2, ns-3, vp1-vp2, and vp2 alone into a recombinant baculovirus and e ... | 1993 | 8380073 |
| comparison of promoter activity in aleutian mink disease parvovirus, minute virus of mice, and canine parvovirus: possible role of weak promoters in the pathogenesis of aleutian mink disease parvovirus infection. | aleutian mink disease parvovirus (adv) infection causes both acute and chronic disease in mink, and we have previously shown that it is the level of viral gene expression that determines the disease pattern. to study the gene regulation of adv, we have cloned the p3 adv and p36 adv promoters in front of a reporter gene, the chloramphenicol acetyltransferase (cat) gene, and analyzed these constructs by transient transfection in a feline kidney cell line and mouse nih 3t3 cells. the genes for adv ... | 1993 | 8383215 |
| cis-acting sequences in the aleutian mink disease parvovirus late promoter important for transcription: comparison to the canine parvovirus and minute virus of mice. | we are currently investigating the regulation of transcription of the aleutian mink disease parvovirus (adv). adv causes a chronic immune complex-mediated condition known as classical aleutian disease, characterized by slow viral replication. this slow replication is an intrinsic property of adv and distinguishes it from the more prototypic parvoviruses such as minute virus of mice (mvm) and canine parvovirus (cpv). we have previously suggested a role for the weak adv promoters in the slow repli ... | 1993 | 8383216 |
| aleutian mink disease parvovirus infection of mink peritoneal macrophages and human macrophage cell lines. | aleutian mink disease parvovirus (adv) mrnas are found in macrophages in lymph nodes and peritoneal exudate cells from adv-infected mink. therefore, we developed an in vitro infection system for adv by using primary cultures of mink macrophages or macrophage cell lines. in peritoneal macrophage cultures from adult mink, virulent adv-utah i strain showed nuclear expression of viral antigens with fluorescein isothiocyanate-labeled adv-infected mink serum, but delineation of specific viral proteins ... | 1993 | 8383229 |
| characterization of chimeric full-length molecular clones of aleutian mink disease parvovirus (adv): identification of a determinant governing replication of adv in cell culture. | the adv-g strain of aleutian mink disease parvovirus (adv) is nonpathogenic for mink but replicates permissively in cell culture, whereas the adv-utah 1 strain is highly pathogenic for mink but replicates poorly in cell culture. in order to relate these phenotypic differences to primary genomic features, we constructed a series of chimeric plasmids between a full-length replication-competent molecular clone of adv-g and subgenomic clones of adv-utah 1 representing map units (mu) 15 to 88. after ... | 1993 | 8396664 |
| aleutian mink disease parvovirus infection of mink macrophages and human macrophage cell line u937: demonstration of antibody-dependent enhancement of infection. | aleutian mink disease parvovirus (adv) infects macrophages in adult mink. the virulent adv-utah i strain, but not the cell culture-adapted adv-g strain, infects mink peritoneal macrophage cultures and the human macrophage cell line u937 in vitro. however, preincubation of adv-g with adv-infected mink serum enhanced its infectivity for u937 cells. the enhancing activity was present in the protein a-binding immunoglobulin g fraction in the serum, but f(ab')2 fragments failed to enhance the infecti ... | 1993 | 8230426 |
| nursing disease in mink: clinical and postmortem findings. | one hundred fifty lactating mink on one ranch in southern ontario were monitored over the lactation period in 1989 for evidence of clinical disease, and serial blood samples were collected for biochemical analysis. antemortem blood samples were collected and necropsies performed on the 17 females that developed nursing disease and on 17 controls matched to the same stage of lactation and on ten nonlactating controls. twenty-two additional nursing disease cases were selected from seven ranches in ... | 1993 | 8116144 |
| topographical analysis of the g virion of aleutian mink disease parvovirus with monoclonal antibodies. | the topography of the aleutian mink disease parvovirus (adv) g virion was analyzed with monoclonal antibodies and polyclonal antiserum. there was homology between the two major structural proteins as others have previously reported. trypsin treatment of the virion with subsequent immunoblotting revealed that vp2 represents the main peptide on the exterior of virion and that vp1 is probably embedded within the capsid. additional analyses of the trypsin-treated virions showed that vp2 is responsib ... | 1992 | 1280944 |
| follicular dendritic cell-b cell interactions in virus disease. common localization but different cell damage caused by antibody immobilized virus? | follicular dendritic cells (fdc) are involved in the trapping and retention of antigen-antibody complexes in lymphoid follicles. this fdc immobilized antigen is thought to be involved in the generation of memory b-lymphocytes. follicular trapping of both aleutian disease virus and hiv particles has been demonstrated. however as far as known their affects on fdc and follicular b-cells are completely different. it is hypothesized that the trapping of (antibody-complexed) virus particles by the fdc ... | 1992 | 1309642 |
| expression of aleutian mink disease parvovirus capsid proteins by a recombinant vaccinia virus: self-assembly of capsid proteins into particles. | a portion of a cdna clone containing coding sequences for both structural proteins (vp1 and vp2) of aleutian mink disease parvovirus (adv) was inserted into recombinant vaccinia viruses, vv:adsp. immunohistochemical staining of vv:adsp-infected cells revealed that the adv antigen was readily detected and localized in the nuclei of infected cells. analysis of vv:adsp-infected cell lystates indicated that both vp1 and vp2 were produced and comigrated with authentic vp1 and vp2 from adv-infected cr ... | 1992 | 1313919 |
| identification of aleutian mink disease parvovirus transcripts in macrophages of infected adult mink. | we examined aleutian mink disease parvovirus (adv) mrna expression in lymph nodes of adult mink infected with adv by northern (rna) blot and in situ hybridization. in northern blot analysis, adv transcripts were detected in the poly(a) rna fraction extracted from mesenteric lymph nodes of two of five mink 10 days after intraperitoneal inoculation with the virulent utah i strain of adv. in strand-specific in situ hybridization, adv dna and mrna were detected in some macrophagelike cells located i ... | 1992 | 1323697 |
| screening for antibodies against aleutian disease virus (adv) in mink. elucidation of dubious results by additive counterimmunoelectrophoresis. | in order to distinguish true positive results in counterimmunoelectrophoresis from false positive ones an additive counterimmunoelectrophoresis was developed. the method was tested on selected mink serum samples as part of a routine testing for antibodies towards aleutian disease virus on 3 million blood samples. the procedure of the method is, that a known positive serum sample is mixed with the patient serum to be tested. the result from a false positive sample will be one precipitin line towa ... | 1992 | 1335756 |
| nucleotide sequence analysis of aleutian mink disease parvovirus shows that multiple virus types are present in infected mink. | different isolates of aleutian mink disease parvovirus (adv) were cloned and nucleotide sequenced. analysis of individual clones from two in vivo-derived isolates of high virulence indicated that more than one type of adv dna were present in each of these isolates. analysis of several clones from two preparations of a cell culture-adapted isolate of low virulence showed the presence of only one type of adv dna. we also describe the nucleotide sequence from map units 44 to 88 of a new type of adv ... | 1991 | 1649336 |
| virus-specific b-lymphocytes are probably the primary targets for aleutian disease virus. | 368 1- to 5-year-old mink of wild-type or black genetic background were infected with aleutian disease virus (adv) naturally or using virus-containing immune complexes or purified virus. thirty of the mink were immunized with dinitrophenol-conjugated ovalbumin (dnp-oa) before and during infection. blood samples were taken at monthly intervals. we found that weak (and transient) monoclonal or oligoclonal immunoglobulin components were present in the plasma or serum approximately 1 month after inf ... | 1991 | 1651029 |
| investigation of mink mhc (mhcmuvi) class i molecules by isoelectric focusing (ief). | mink (mustela vision) class i leucocyte antigens, here abbreviated mhcmuvi according to the proposal given by klein et al. (1990), were characterized by isoelectric focusing (ief), using triton x-114-extracted and neuraminidase-treated membrane proteins from spleen cells, followed by immunoblotting and development with a murine monoclonal antibody raised against denatured major histocompatibility complex (mhc) class i antigens. muvi class i antigens were investigated in a group of 97 danish mink ... | 1991 | 1681893 |
| genetic polymorphism of igg in the mink. 7. expression of the c gamma-allotypes in domestic mink infected with the aleutian disease virus. | the aleutian disease (ad), i.e., viral plasmocytosis in mink can be used as a model of the natural development of immune complex pathology in man. the immunogenetic aspect of ad was studied with the help of genetic markers of the constant region of the mink immunoglobulin gamma-heavy chain (the c gamma allotypes h2, h3, h4, h6, h7 and h8). the frequencies of 2 of the 6 allotypes, h3 and h4, were significantly higher in the ad-infected than in normal minks from the same population. this supports ... | 1991 | 1789990 |
| replication of aleutian mink disease parvovirus in lymphoid tissues of adult mink: involvement of follicular dendritic cells and macrophages. | by using strand-specific in situ hybridization and immunohistochemistry, evidence for replication of the aleutian mink disease parvovirus was observed in cells resembling macrophages and cells resembling follicular dendritic cells at 10 days after infection but only in macrophages at 60 days. sequestration of the aleutian mink disease parvovirus in larger numbers of macrophages and follicular dendritic cells was noted at both 10 and 60 days. | 1991 | 1846208 |
| [activation of expression of two immunoglobulin sn-genes in the american mink with aleutian disease]. | 110 ranch-raised minks were injected with the aleutian disease virus. allotypes of constant regions of gamma-heavy chains of the mink immunoglobulins secreted have been analysed during 3 months. activation of the expression of two markers (h3 and/or h4) up to minor or to nominal level (above 200 micrograms/ml) was observed. no such enhancement of expression of two other allotypes (h6 and h8) was found. the results suggest that the expression of two mink immunoglobulin ch genes induced by viral i ... | 1991 | 1916255 |
| simultaneous identification of viral proteins and nucleic acids in cells infected with aleutian mink disease parvovirus. | a method combining in situ hybridization and immunohistochemistry was used to characterize cells infected with aleutian mink disease parvovirus (adv). single-stranded rna hybridization probes specific for obligate replicative intermediates and antisera specific for virion or non-structural proteins were employed. crandell feline kidney cells in which the adv-g strain of adv was permissively replicating contained virion and non-structural proteins, large amounts of single stranded virion dna, dup ... | 1990 | 1965846 |
| mechanisms contributing to the virus persistence in aleutian disease. | in this review published results and further studies concerning the persistence of aleutian disease virus (adv) isolate sl3 are presented. by southern blot and in situ hybridization with strand-specific rna probes focal replication of adv-dna was demonstrated in spleen, mesenteric lymph nodes, sporadically in mononuclear cells of the peripheral blood and bone marrow cells. these findings further support the concept of the lymphotropism of adv. all cell culture-adapted adv strains appear to have ... | 1990 | 2155772 |
| the monomer covalently closed linear replicative form dna is an intermediate of aleutian disease parvovirus dna replication. | in this report we present data indicating that the recently detected monomer covalently closed linear replicative form dna (mccl rf dna) is an intermediate of aleutian disease virus (adv) dna replication. this dna molecule is characterized by covalently closed terminal hairpins on either end of the linear ds genomic molecule. its first detection early after infection in vitro, the association with adv-specific replication complexes and the de novo synthesis of mccl rf dna by isolated replication ... | 1990 | 2155994 |
| proteins of viral and cellular origin bind to the aleutian disease virus (adv) dna 3'-terminal hairpin: presentation of a scheme for encapsidation of adv dna. | we have observed the binding of viral and cellular proteins to the aleutian disease virus (adv) 3' terminus of replicative-form dna. gel retardation assays showed specific band shifts produced by whole-cell extracts from either adv-infected or uninfected cells, as well as band reduction produced by adv capsids. in all cases, binding was confined to the turnaround, t-shaped terminal form; no binding to the extended conformation of replicative-form dna was detected. this indicates the importance o ... | 1990 | 2157046 |
| analysis of experimental mink enteritis virus infection in mink: in situ hybridization, serology, and histopathology. | strand-specific hybridization probes were used in in situ hybridization studies to localize cells containing mink enteritis virus (mev) virion dna or mev replicative-form dna and mrna. following the experimental mev infection of 3-month-old unvaccinated mink, a significant increase in serum antibodies to mev was detected at postinfection day (pid) 6, 2 days after the onset of fecal shedding of virus. prior to the appearance of virus in feces, viral dna could be detected in the mesenteric lymph n ... | 1990 | 2159543 |
| pathogenesis of disease caused by aleutian mink disease parvovirus. | a review of the pathogenesis of aleutian mink disease parvovirus (adv) infection based on recent knowledge gained by the author and collaborators is given. the review focuses mainly on the following topics. 1) development of an easy, sensitive and fast assay for detection of adv antigens and antibodies directed against these antigens. a highly sensitive rocket line immunoelectrophoretic assay (rlie) was developed. this assay turned out to be 32 times more sensitive than the counter current elect ... | 1990 | 2159766 |
| nucleotide sequence of the 5'-terminal palindrome of aleutian mink disease parvovirus and construction of an infectious molecular clone. | the 5'-terminal palindrome of the adv-g strain of aleutian mink disease parvovirus (adv) was molecularly cloned and sequenced. a full-length molecular clone of adv-g, denoted pxvb, was then constructed. when this clone was transfected into cell cultures, infectious adv could be rescued. virus derived from pxvb was nonpathogenic for adult mink, as is the parent adv-g strain. | 1990 | 2161958 |
| violet mink develop an acute disease after experimental infection with aleutian disease virus (adv) isolate adv sl3. | six-aleutian (aa)-genotype violet mink were infected intraperitoneally with the aleutian disease virus (adv) bone marrow derived isolate adv sl3. all animals developed virus-specific antibodies and hypergammaglobulinaemia. mortality during the fourteen week duration of the infection was 50%. the virus induced (histo)pathological lesions typical for aleutian disease. by immunohistochemical examination using a virus capsid-specific monoclonal antibody viral antigen was detected in lymph nodes, spl ... | 1990 | 2163578 |
| passive transfer of antiviral antibodies restricts replication of aleutian mink disease parvovirus in vivo. | when mink kits were infected neonatally with a highly virulent strain of aleutian disease virus (adv), 100% of both aleutian and non-aleutian genotype mink died of interstitial pneumonia characterized by permissive adv infection of alveolar type ii cells. treatment of infected kits with either mink anti-adv gamma globulin or mouse monoclonal antibodies against adv structural proteins reduced mortality by 50 to 75% and drastically reduced the severity of clinical signs. interestingly, mink kits t ... | 1989 | 2535756 |
| a novel replicative form dna of aleutian disease virus: the covalently closed linear dna of the parvoviruses. | the analysis of replicative form (rf) dna of aleutian disease virus (adv) by alkaline gel electrophoresis revealed that all rf dna species segregate into dna single strands which represent integral multiples of a genome equivalent. this demonstrates that as with other autonomous parvoviruses, the virion and complementary dna strands are frequently linked by hairpin structures and that also, nicks are present at subterminal sites. approximately 50% of the 5'-terminal hairpins contain a subtermina ... | 1989 | 2543770 |
| analysis of parvovirus infections using strand-specific hybridization probes. | the autonomous parvoviruses cause a broad spectrum of acute and chronic infections of animals and man. the discrimination of sites of viral replication from sites of viral sequestration is an important goal in elucidating the pathogenesis of these diseases. it is possible to employ strand-specific rna hybridization probes in such analyses because a 'plus' sense probe will react with single stranded virion dna and duplex replicative form dna, but a 'minus' sense probe will react preferentially wi ... | 1989 | 2554612 |
| [restriction analysis of the dna of aleutian mink disease virus strain p-1]. | the ii-1 strain of the aleutian disease virus (adv-ii-1) was isolated from experimentally infected mink organs. the viral particles were isolated having 23 to 24 nm in diameter with the buoyant density of the virions in cscl gradient being 1.41 g.ml-1. the single stranded adv dna extracted from the purified virus particles had the molecular mass about 1.4 . 10(6) (4800 bases). the double-stranded replicative form of adv dna has been synthesized in vitro with the use of a large "klenow" fragment ... | 1989 | 2561377 |
| mink with aleutian disease have autoantibodies to some autoantigens. | thirty mink infected with aleutian disease virus (adv) were found to have elevated levels of antibody to double-stranded dna (dsdna) in their sera when compared to 30 healthy mink. the anti-dsdna antibody levels in the diseased mink were, however, not found to correlate with the total amount of immunoglobulin. this was a common observation for all autoantibodies tested. the concentration of rheumatoid factors of igg class, but not those of igm class, was found to be significantly higher in the d ... | 1989 | 2617848 |
| mink infected with aleutian disease virus have an elevated level of cd8-positive t-lymphocytes. | lymphocytes, monocytes, granulocytes, b-lymphocytes and cd8-positive t-lymphocytes of non-infected mink and mink infected with aleutian disease virus (adv) were measured by flow cytometry. the gammaglobulin levels of the sera were also measured. besides development of hypergammaglobulinaemia in the infected mink, the most pronounced finding was that the number of cd8-positive lymphocytes doubled on average during development of aleutian disease, while the number of b-lymphocytes did not change d ... | 1989 | 2785727 |
| detection of aleutian disease virus dna in tissues of naturally infected mink. | organs of naturally infected mink were examined for the presence of aleutian disease virus (adv) dna by in situ hybridization. spleen, lymph nodes, thymus, bone marrow, kidney, liver, lung and small intestine were found to be positive for adv to differing extents. infected lymphoid organs showed a focal distribution of positive cells. southern blot analysis of dna extracted from infected organs revealed replicative forms of viral dna in spleen and bone marrow samples only. these findings are con ... | 1988 | 2832533 |
| nucleotide sequence and genomic organization of aleutian mink disease parvovirus (adv): sequence comparisons between a nonpathogenic and a pathogenic strain of adv. | a dna sequence of 4,592 nucleotides (nt) was derived for the nonpathogenic adv-g strain of aleutian mink disease parvovirus (adv). the 3'(left) end of the virion strand contained a 117-nt palindrome that could assume a y-shaped configuration similar to, but less stable than, that of other parvoviruses. the sequence obtained for the 5' end was incomplete and did not contain the 5' (right) hairpin structure but ended just after a 25-nt a + t-rich direct repeat. features of adv genomic organization ... | 1988 | 2839709 |
| capsid protein vp1 (p85) of aleutian disease virus is a major dna-binding protein. | the interaction between aleutian disease virus (adv) dna and proteins isolated from adv-infected cells or adv virions, respectively, was examined. proteins were separated on sds-polyacrylamide gels, transferred to nitrocellulose, and probed with 32p-labeled restriction fragments of replicative form (rf) dna or with single-stranded virion dna. the adv capsid protein vp1 was found to bind the 3'-terminal bamhi fragment of rf dna extending from map units (m.u.) 0 to 15. no binding was observed with ... | 1988 | 2842956 |
| detailed transcription map of aleutian mink disease parvovirus. | we studied the transcription program of aleutian mink disease parvovirus (adv) by using a combination of cdna cloning and sequencing, primer extension, and northern (rna) blot hybridization with splice-specific oligonucleotides. the 4.8-kilobase adv genome was transcribed in the rightward direction, yielding plus-sense polyadenylated transcripts of 4.3 (r1 rna), 2.8 (r2), 2.8 (r3), 1.1 (rx), and 0.85 (r2') kilobases. each rna transcript had potential translation initiation sites within open read ... | 1988 | 2843669 |
| treatment of neonatally aleutian disease virus (adv) infected mink kits with gammaglobulin containing antibodies to adv reduces the death rate of mink kits. | 1988 | 2476006 | |
| pregnancy rate and foetal mortality in aleutian disease virus infected mink. | 1988 | 3223478 | |
| studies on the sequential development of acute interstitial pneumonia caused by aleutian disease virus in mink kits. | we studied different parameters during the development of acute interstitial pneumonia in mink kits caused by neonatal infection with aleutian disease virus (adv). when histological lesions, presence of intranuclear inclusion bodies, and intranuclearly localized adv antigen were correlated with levels of single-stranded virion and duplex replicative forms of adv dna in the different tissues, it was concluded that the lung, probably alveolar type ii cells, is the major primary target for viral re ... | 1987 | 3023709 |
| apparent lack of neutralizing antibodies in aleutian disease is due to masking of antigenic sites by phospholipids. | it is generally accepted that aleutian disease virus (adv) cannot be neutralized by antibodies either in vivo or in vitro. we found several ways to demonstrate neutralization of adv by specific antibodies from mink. it was essential to make adv monodisperse by treatment with sodium lauroyl sarkosyl or n-butanol or by filtration through 0.05-micron membranes before neutralization tests. in kinetic experiments, there was a 95% loss of virus infectivity within the first 5 min of reaction, but a res ... | 1987 | 2437696 |
| restricted viral antibody specificity in many ferrets infected with the ferret aleutian disease parvovirus. brief report. | the majority of ferrets infected with a ferret strain of aleutian disease virus (adv) produce antibody only to a detergent-sensitive common determinant on the two closely related virion proteins. ferrets with high antibody titers and mink infected with this virus also produce antibody to one or more virion immunogenic determinants unaffected by detergent. | 1987 | 2434063 |
| lymphotropic strain sl3 of aleutian disease virus: identification of replicative form dna, molecular cloning and expression of capsid-specific proteins. | replicative form (rf) dna of the lymphotropic strain sl3 of aleutian disease virus was isolated from infected cell cultures. a novel intermediate of about 7.6 kilobases was demonstrated in hirt lysates in addition to single-stranded viral, double-stranded monomer and dimer rf dna. the monomer rf dna exhibited a length heterogeneity of 70 bp and 160 bp at its 3' and 5' termini. the two major monomer rf dna species each contained hairpins in the extended or the foldback configurations. a central f ... | 1987 | 3033124 |
| in situ molecular hybridization for detection of aleutian mink disease parvovirus dna by using strand-specific probes: identification of target cells for viral replication in cell cultures and in mink kits with virus-induced interstitial pneumonia. | strand-specific hybridization probes were utilized in in situ molecular hybridization specifically to localize replicative form dna of aleutian mink disease parvovirus (adv). throughout in vitro infection, duplex replicative form dna of adv was located in the cell nuclei. single-stranded virion dna and capsid proteins were present in the nuclei early in infection, but were later translocated to the cytoplasm. in neonatal mink, adv causes acute interstitial pneumonia, and replicative forms of vir ... | 1987 | 3037104 |
| epitopic mapping of structural and nonstructural aleutian disease virus proteins. | common antigenic properties for p85 and p75 but a different antigenic character for p71 aleutian disease virus (adv) proteins were demonstrated by western blot analysis with monoclonal antibodies. it was shown that four hybridomas (adv-hy 47, 66, 77 and 84) with specific reactivity for structural proteins p85 and p75 also recognized p25 but not the p71, nonstructural, protein. in turn, the monoclonal antibody adv-hy 2 recognized the p71 protein only. for further studies of their antigenic proper ... | 1986 | 2437069 |
| restricted heterogeneity of the early antibody response to aleutian disease virus in mink kits. | mink kits born of aleutian disease (ad) negative dams were infected neonatally with different isolates of aleutian disease virus (adv). they were then sacrificed at different days after infection (10-45 days) and their sera were analysed by different techniques for concentrations of immunoglobulins and quality of the produced antibodies to adv. the infected mink kits produced significantly higher concentrations of igm and igg immunoglobulins than non-infected-age matched control mink (p less tha ... | 1986 | 3024449 |
| replication of aleutian disease virus in mink lymphocytes infected in vitro. | the kinetics of aleutian disease virus (adv) replication in mink lymphocytes was followed by the analysis of virus-specific antigens, infectious virus, and viral dna. stimulation with pokeweed mitogen (pwm; 20 micrograms/ml) increased the synthesis of adv dna in cultivated cells of the b-cell fraction. maximum virus titers [10(6.5) fluorescence-forming units (ffu)/ml] were achieved after incubating infected cells for 60 h at 32 degrees. at this time, 10.4% of the cells in the b-cell-enriched fra ... | 1986 | 3026990 |
| monoclonal antibodies against aleutian disease virus distinguish virus strains and differentiate sites of virus replication from sites of viral antigen sequestration. | monoclonal antibodies (mabs) were used to study antigenic differences among strains of aleutian disease virus (adv) and to characterize viral proteins in vitro and in vivo. a number of adv field strains could be discriminated, and highly virulent utah i adv was clearly delineated from the tissue culture-adapted avirulent adv-g strain. this specificity could be demonstrated by indirect immunofluorescence against infected cultures of crandell feline kidney cells or against tissues of utah i adv-in ... | 1986 | 3001352 |
| demonstration of non-degraded aleutian disease virus (adv) proteins in lung tissue from experimentally infected mink kits. brief report. | isolates of adv replicate to rather high quantities in lungs from neonatally infected mink kits. the virus was analysed for polypeptide composition, and for the first time high molecular weight polypeptides have been observed in in vivo produced advs. these polypeptides are analogous to those of in vitro produced advs. the molecular weights of the structural polypeptides of the low virulence pullman adv and the highly virulent dk and utah i isolates of adv were found to be 88kd and 78kd and in v ... | 1986 | 3002299 |
| acute interstitial pneumonia in mink kits: experimental reproduction of the disease. | organ homogenates from kits that died of interstitial pneumonia were inoculated into adult aleutian disease virus (adv)-negative mink and shown to contain infectious adv. acute interstitial pneumonia was experimentally reproduced with the organ homogenate but only by inoculation of newborn kits born from adv-negative dams. older kits and kits from adv-positive dams did not develop interstitial pneumonia, but later developed the classic form of aleutian disease. electron microscopic examination w ... | 1986 | 3022453 |
| interferon response in normal and aleutian disease virus-infected mink. | studies were done to determine whether differences in interferon production are responsible for the resistance of pastel mink to aleutian disease. the abilities of normal pastel and sapphire mink to produce interferon when inoculated with either newcastle disease virus or a synthetic polyribonucleotide, poly (i):poly (c), were identical, even to the production of a novel, acid-labile interferon. the resistance of pastel mink to aleutian disease did not correlate with interferon production, becau ... | 1986 | 2426469 |
| mink parvoviruses and interferons: in vitro studies. | although interferons can inhibit the replication of a number of viruses, little is known about their ability to inhibit parvovirus replication. therefore, in vitro experiments were done to determine if aleutian disease virus and mink enteritis virus, two autonomously replicating mink parvoviruses, induced interferon, were sensitive to the effects of interferon, or inhibited the production of interferon. the results indicated that these parvoviruses neither induced nor were sensitive to the effec ... | 1986 | 2431162 |
| analysis of aleutian disease virus infection in vitro and in vivo: demonstration of aleutian disease virus dna in tissues of infected mink. | aleutian disease virus (adv) infection was analyzed in vivo and in vitro to compare virus replication in cell culture and in mink. initial experiments compared cultures of crandell feline kidney (crfk) cells infected with the avirulent adv-g strain or the highly virulent utah i adv. the number of adv-infected cells was estimated by calculating the percentage of cells displaying adv antigen by immunofluorescence (ifa), and several parameters of infection were determined. infected cells contained ... | 1985 | 3894686 |
| rocket line immunoelectrophoresis: an improved assay for simultaneous quantification of a mink parvovirus (aleutian disease virus) antigen and antibody. | a rocket line immunoelectrophoretic assay (rlie) was developed for the simultaneous quantification of viral antigens and antiviral antibodies of the important mink parvovirus, aleutian disease virus (adv). the sensitivity of the rlie assay was found to be 5 log2 higher than that of the counter current immunoelectrophoresis which is the assay routinely used for diagnostic purposes. | 1985 | 2984229 |
| temporal replication of the pullman strain of aleutian disease virus in royal pastel mink. | information was sought on the temporal replication of aleutian disease virus in 27 royal pastel mink. groups of three were examined 8 to 126 days after they were inoculated subcutaneously with 10(3) 50% lethal doses of the pullman strain. much individual variation was noted in the onset of infection, occurrence of viremia, and extent of virus replication in the tissues. thus, virus was detected in lymph nodes regional to the site of inoculation in only some mink during the first 14 days after in ... | 1985 | 2991603 |
| experimental transmission of aleutian disease virus (adv) to different animal species. | two animals from each of 8 different species (mink, finn raccoon, cat, dog, ferret, blue fox, mouse and rabbit) were inoculated with the highly virulent utah i strain of adv. only the mink developed hypergammaglobulinemia. all animals produced antibodies to adv antigens, but with different titres. mink sera had much higher antibody titres than the other animal sera. antibodies to the adv-coded, non-structural polypeptide (p71) were found in mink, finn raccoons and dogs only. presence of this kin ... | 1985 | 2994362 |
| royal pastel mink respond variously to inoculation with aleutian disease virus of low virulence. | information was sought on the varied responses of royal pastel mink (a non-aleutian genotype) to aleutian disease virus of low virulence. thus, of 20 yearling female pastel mink inoculated subcutaneously with a large amount of the pullman strain of aleutian disease virus, only 3 succumbed to the disease. of the other 17 mink, 3 had neither viremia nor a rise in level of serum gamma globulin during the 24 weeks after inoculation. the other 14 mink were viremic for variable periods during the firs ... | 1984 | 6199516 |
| serological analyses of different mink aleutian disease virus strains. | four different isolates of aleutian disease virus have been compared electrophoretically and serologically. these were the dk and utah 1 isolates, known as highly virulent strains, the pullman isolate, known as a low virulent strain and the avirulent adv-g isolate, which is the only strain grown in cell culture. adv-g was shown to migrate in agarose electrophoresis 22 per cent slower than the other strains. several murine monoclonal antibodies were prepared against each of the isolates. each one ... | 1984 | 6200092 |
| aleutian disease virus, a parvovirus, is proteolytically degraded during in vivo infection in mink. | the polypeptides of the highly virulent mink-passaged utah i and the nonvirulent cell culture-adapted adv-g strain of aleutian disease virus (adv) were compared. when crfk cells infected with either utah i or adv-g were analyzed by immunoprecipitation, both viruses induced proteins with molecular weights characteristic of the adv-g 85,000 ( 85k )- and 75k-dalton structural proteins (p85 and p75) as well as the 71k -dalton nonvirion protein p71 . however, when utah i, pullman adv, and dk adv (a d ... | 1984 | 6202887 |
| immunoenzyme western blotting analysis of antibody specificity in aleutian disease of mink, a parvovirus infection. | aleutian disease virus (adv), an autonomous parvovirus, persistently infects mink and induces very high levels of virus-specific antibody. all strains of adv infect all mink, but only highly virulent strains cause progressive disease in non-aleutian mink. the development of antibody to individual adv proteins was evaluated by western blotting by using the sera of 22 uninfected mink and 163 naturally or experimentally infected mink. adv has virion proteins of 86,000 and 78,000 daltons that are cl ... | 1984 | 6092706 |
| acute interstitial pneumonitis caused by aleutian disease virus in mink kits. | in four danish mink ranches acute interstitial pneumonitis caused excessive mortality among kits within the first 2 1/2 months after parturition. the disease was found to be due to an aleutian disease virus (adv) and could be reproduced experimentally in neonatal kits by inoculation with material from spontaneous cases, as well as with other strains of adv. experimental reproduction was only possible in kits from dams free of aleutian disease (ad) whereas kits from dams experimentally or natural ... | 1984 | 6095587 |
| aleutian disease virus in b and t lymphocytes from blood and spleen and in bone marrow cells from naturally infected mink. | in the nuclei of 4% of peripheral blood or spleen mononuclear cells (mnc), aleutian disease virus(adv)-specific antigens were found by a direct immunofluorescence test. the mnc were further fractionated by nylon wool, affinity chromatography using staphylococcus aureus protein, or percoll gradient techniques. adv and specific antigens were detected in mnc fractions enriched in either the b or t lymphocytes. in the bone marrow, up to 40% antigen-positive cells were demonstrated over a period of 1 ... | 1984 | 6096293 |
| aleutian disease in mink: virology, immunology and pathogenesis. | aleutian disease is an immunological disease of mink caused by a persistent virus infection. recently aleutian disease viral antigen has been extracted from tissue of mink early in the course of infection and the aleutian disease virus has been isolated. the virus particles were similar in morphology and size to certain parvovirus having a 23 nm diameter, spherical shape and icosahedral capsid. recent developments in serological techniques, i.e. immunofluorescence, complement fixation, countercu ... | 1984 | 6096541 |
| examination of aleutian disease virus in charge-shift crossed immunoelectrophoresis. | the surface properties of aleutian disease virus were studied by charge-shift crossed immunoelectrophoresis. when different strains of aleutian disease virus were treated with non-charged detergent followed by charged detergents, they showed bi-directional migration velocity shifts in electrophoresis, indicating amphiphilic surface properties of the virus. | 1984 | 6099679 |
| much of the increased igg in aleutian disease of mink is viral antibody. | aleutian disease (ad) is caused by a persistent infection of mink with an autonomous parvovirus. chronically infected mink develop widespread plasmacytosis, a marked elevation of their serum igg, and immune complex disease. a substantial fraction of the igg in the serum of mink with aleutian disease may be specifically absorbed by monolayer cell cultures infected with aleutian disease virus. the maximum percentage of absorption of igg found was 81% in a mink with 5.4 g/dl of igg. mink with the m ... | 1984 | 6100982 |
| immunoglobulin classes of aleutian disease virus antibody. | aleutian disease virus (adv) persistently infects mink and causes marked hypergammaglobulinemia. immunoglobulin class-specific antisera were used to define the total immunoglobulin of each class by radial immunodiffusion and the immunoglobulin class of adv-specific antibody by immunofluorescence in experimentally and naturally infected mink. electrophoretic gamma globulin closely reflects the immunoglobulin g (igg) level in mink, and the majority of the increased immunoglobulin and adv antibody ... | 1984 | 6319283 |
| analysis of the quantity of antiviral antibodies from mink infected with different aleutian disease virus strains. | mink persistently infected with aleutian disease virus (adv) develop hypergammaglobulinaemia and immune complex disease. radiolabelled antibodies from mink infected with adv-g, dk, pullman , and utah i strains of adv were reacted against all four adv strains in radioimmunoassay (ria). the amount of anti-adv antibody in two equally hypergammaglobulinaemic serum pools varied from 13% (anti- pullman ) to 57% (anti-utah i). serum pools from two other sources (anti-dk and anti-adv-g), although less h ... | 1984 | 6328641 |
| mink with aleutian disease have high-affinity antiviral antibodies. | mink persistently infected with aleutian disease virus (adv) develop plasmacytosis (hypergammaglobulinaemia) and immune complex disease. mink of different colour phases were infected with different strains of adv and bled at different times after infection. the average antibody affinities (kav) were measured in the sera and found to fall in the range of 2 x 10(9) - 2 x 10(10) m-1, thus indicating good-quality antibodies. in sera of non-aleutian genotype mink a decline in kav during development o ... | 1984 | 6328642 |
| immune complexes in aleutian disease: demonstration of antibody on isolated virus. | the specific binding of staphylococcal protein a for mammalian immunoglobulin g was used to demonstrate igg associated with aleutian disease virus (adv) when isolated from infected mink tissues. protein a specifically bound to mink serum ig with no reaction with other serum or tissue proteins. protein a labeled with 131iodine reacted with crude virus preparations but not with virus that had been purified by freon extraction to the point where it became reactive with antibody by counterimmunoelec ... | 1984 | 6328738 |
| isolation of virus and antibody containing immune complexes from mink with aleutian disease by affinity chromatography of equine complement clq. | affinity chromatography on immobilized equine complement clq was used for the isolation of complement-binding immune complexes in sera of mink infected with aleutian disease virus. immune complexes were isolated and quantitated from 4 of 5 infected mink, as early as 2 weeks after infection and before hypergammaglobulinemia had appeared. the quantity of immunoglobulin g in these immune complexes ranged from 180 to 370 micrograms/ml serum. there were no clq-binding immune complexes found in mink w ... | 1983 | 6297342 |
| characterization of the aleutian disease virus genome and its intracellular forms. | aleutian disease virus (adv) of mink is a nondefective parvovirus with a single-stranded dna genome. we characterized the viral dna forms found in infected cells prepared by a modified hirt extraction procedure. double-stranded dna molecules corresponding in size to 4.8-kilobase-pair duplex monomers and 9.6-kilobase-pair duplex dimers were identified in agarose gels by blot hybridization to 32p-labeled adv dna. a rapidly reannealing adv duplex monomer was isolated on a preparative scale and phys ... | 1983 | 6300467 |