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gorham-stout disease of the skull base with hearing loss: dramatic recovery and antiangiogenic therapy.gorham-stout disease (gsd) is a rare disorder of unknown etiology. we present a 6-year-old male with gsd involving the skull base who presented with recurrent cerebrospinal fluid (csf) rhinorrhea, severe hearing loss, and facial palsy secondary to cerebellar herniation into the internal auditory canal. after 2 months of treatment with pegylated interferon (ifn) α-2b (50 μg/week), his hearing recovered dramatically. two years later, new bone formation appeared radiologically and ifn was switched ...201626713883
gorham-stout disease: radiological, histological, and clinical features of 12 cases and review of literature.gorham-stout disease (gsd) is an exceedingly rare disease characterized by progressive osteolysis and angiomatosis. we investigate the features of this disease and evaluate the effects of bisphosphonates (bps) on it. the clinical, radiological, and pathological characteristics of 12 patients diagnosed with gsd were summarized. immunohistochemical staining with specific lymphatic endothelial markers (d2-40), vascular markers (cd 31, cd 34), and vascular endothelial growth factor (vegf) and vascul ...201625227772
the stiffening of the cell walls observed during physiological softening of pears.the young's modulus of the primary cell walls of pears decreases linearly during the pre-harvest on-tree maturation and increases during postharvest storage, and does not correlate with firmness of fruit. the determination of mechanical properties of cell walls is indispensable for understanding the mechanism of physiological softening and deterioration of quality of fruits during postharvest storage. the young's modulus of the primary cell walls from pear fruit (pyrus communis l., cultivars 'co ...201626498014
genomics of pear and other rosaceae fruit trees.the family rosaceae includes many economically important fruit trees, such as pear, apple, peach, cherry, quince, apricot, plum, raspberry, and loquat. over the past few years, whole-genome sequences have been released for chinese pear, european pear, apple, peach, japanese apricot, and strawberry. these sequences help us to conduct functional and comparative genomics studies and to develop new cultivars with desirable traits by marker-assisted selection in breeding programs. these genomics reso ...201627069399
[spontaneous idiopathic osteolysis (gorham syndrome). a case report].the spontaneous idiopathic osteolysis is know as a slow progressive absorbtion process of the bone with unknown causes. it is supposed that in the "gorham-syndrom" a hämangiomatosis of the bone exists. in some cases of spontaneous osteolysis there wasn't any vascular proliferation noted. one case with spontaneous osteolysis will be discussed. the histological picture of this patient is described as a chronic uspecific osteoarthritis. another case could be documented radiologically at the left di ...20166516542
[idiopathic massive osteolysis of jackson-gorham disease. apropos of a recent case]. 20164278890
molecular cloning and functional analysis of a uv-b photoreceptor gene, mduvr8 (uv resistance locus 8), from apple.uvr8 (uv resistance locus 8) is an ultraviolet-b (uv-b; 280-315nm) light receptor that is involved in regulating many aspects of plant growth and development. uv-b irradiation can increase the development of flower and fruit coloration in many fruit trees, such as grape, pear and apple. previous investigations of the structure and functions of uvr8 in plants have largely focused on arabidopsis. here, we isolated the uvr8 gene from apple (malus domestica) and analyzed its function in transgenic a ...201627095405
gene characterization and transcription analysis of two new ammonium transporters in pear rootstock (pyrus betulaefolia).ammonium is the primarily nitrogen source for plant growth, but the molecular basis of ammonium acquisition in fruit species remains poorly understood. in this study, we report on the characterization of two new ammonium transporters (amt) in the perennial tree pyrus betulaefolia. in silico analyses and yeast complementation assays revealed that both pbamt1;3 and pbamt1;5 can be classified in the amt1 sub-family. the specific expression of pbamt1;3 in roots and of pbamt1;5 in leaves indicates th ...201626943161
two myb transcription factors regulate flavonoid biosynthesis in pear fruit (pyrus bretschneideri rehd.).flavonoid compounds play important roles in the modern diet, and pear fruits are an excellent dietary source of these metabolites. however, information on the regulatory network of flavonoid biosynthesis in pear fruits is rare. in this work, 18 putative flavonoid-related myb transcription factors (tfs) were screened by phylogenetic analysis and four of them were correlated with flavonoid biosynthesis patterns in pear fruits. among these myb-like genes, the specific functions of two novel myb tfs ...201626687179
dormancy-associated mads-box genes and micrornas jointly control dormancy transition in pear (pyrus pyrifolia white pear group) flower bud.bud dormancy in perennial plants is indispensable to survival over winter and to regrowth and development in the following year. however, the molecular pathways of endo-dormancy induction, maintenance, and release are still unclear, especially in fruit crops. to identify genes with roles in regulating endo-dormancy, 30 mikc(c)-type mads-box genes were identified in the pear genome and characterized. the 30 genes were analysed to determine their phylogenetic relationships with homologous genes, g ...201626466664
the impact of a cold chain break on the survival of salmonella enterica and listeria monocytogenes on minimally processed 'conference' pears during their shelf life.in recent years, improved detection methods and increased fresh-cut processing of produce have led to an increased number of outbreaks associated with fresh fruits and vegetables. during fruit and vegetable processing, natural protective barriers are removed and tissues are cut, causing nutrient rich exudates and providing attachment sites for microbes. consequently, fresh-cut produce is more susceptible to microbial proliferation than whole produce.201627813092
exploitation of leuconostoc mesenteroides strains to improve shelf life, rheological, sensory and functional features of prickly pear (opuntia ficus-indica l.) fruit puree.strains of leuconostoc mesenteroides were identified from raw prickly pear (opuntia ficus-indica l.). five autochthonous strains were selected based on the kinetics of growth and acidification on prickly pear fruit juice, and the capacity to synthesize exo-polysaccharides. all selected leuc. mesenteroides strains showed an in vitro mucilage-degrading capability. a protocol for processing and storage of fermented prickly pear fruit puree (fp) was set up. unstarted fp and chemically acidified fp w ...201627375258
lactic acid fermentation of cactus cladodes (opuntia ficus-indica l.) generates flavonoid derivatives with antioxidant and anti-inflammatory properties.cactus pear (opuntia ficus-indica l.) is widely distributed in the arid and semi-arid regions throughout the world. in the last decades, the interest towards vegetative crop increased, and cladodes are exploited for nutraceutical and health-promoting properties. this study aimed at investigating the capacity of selected lactic acid bacteria to increase the antioxidant and anti-inflammatory properties of cactus cladodes pulp, with the perspective of producing a functional ingredient, dietary supp ...201627023062
characterization and 1.57 å resolution structure of the key fire blight phosphatase amsi from erwinia amylovora.amsi is a low-molecular-weight protein tyrosine phosphatase that regulates the production of amylovoran in the gram-negative bacterium erwinia amylovora, a specific pathogen of rosaceous plants such as apple, pear and quince. amylovoran is an exopolysaccharide that is necessary for successful infection. in order to shed light on amsi, its structure was solved at 1.57 å resolution at the same ph as its highest measured activity (ph 5.5). in the active site, a water molecule, bridging between the ...201627917839
transcriptional response of erwinia amylovora to copper shock: in vivo role of the copa gene.fire blight is a devastating plant disease caused by the bacterium erwinia amylovora, and its control is frequently based on the use of copper-based compounds whose mechanisms of action are not well known. consequently, in this article, we investigate the response of e. amylovora to copper shock by a whole-genome microarray approach. transcriptional analyses showed that, in the presence of copper, 23 genes were increased in expression; these genes were classified mainly into the transport and st ...201627862834
the in planta proteome of wild type strains of the fire blight pathogen, erwinia amylovora.erwinia amylovora is a gram-negative plant pathogen that causes fire blight. this disease affects most members of the rosaceae family including apple and pear. here, an infection model is introduced to study proteomic changes in a highly virulent e. amylovora strain upon interaction with its host as compared to a lower virulent strain. for this purpose separate shoots of apple rootstocks were wound-infected and when infection became systemic, bacterial cells were isolated and processed for analy ...201626924300
phylogenetic analysis of pr genes in some pome fruit species with the emphasis on transcriptional analysis and ros response under erwinia amylovora inoculation in apple.attempts were made to identify eight pathogenesis related (pr) genes (i.e., pr-1a, pr3-ch1, pr3-ch2, pr3-ch3, pr3-ch4, pr3-ch5, pr-5 and pr-8) from 27 genotypes of apple, quince and pear, which are induced in response to inoculation with the pathogen erwinia amylovora, the causal agent of fire blight. totally, 32 pr genes of different families were obtained, excepting pr3-ch2 (amplified only in apple) and pr3-ch4 (amplified only in apple and pear), the others were successfully amplified in all t ...201626589211
proteomic and ultrastructural analysis of the effect of a new nitazoxanide-n-methyl-1h-benzimidazole hybrid against giardia intestinalis.in an effort to develop alternative drugs for the treatment of giardiasis our research group has synthesized and evaluated a novel nitazoxanide and n-methyl-1h-benzimidazole hybrid molecule, named cmc-20. it showed an ic50 of 0.010 μm on giardia intestinalis, lower than the ic50 values of 0.015, 0.037 and 1.224 μm for nitazoxanide, albendazole and metronidazole, respectively. in addition, we report studies carried out on its mechanism of action and effect at the ultrastructural level on g. intes ...201627033928
integration host factor is required for rpon-dependent hrpl gene expression and controls motility by positively regulating rsmb srna in erwinia amylovora.erwinia amylovora requires an hrp-type iii secretion system (t3ss) to cause disease. it has been reported that hrpl, the master regulator of t3ss, is transcriptionally regulated by sigma factor 54 (rpon), yhbh, and hrps. in this study, the role of integration host factor (ihf) in regulating hrpl and t3ss gene expression was investigated. ihf is a nucleoid-associated protein that regulates gene expression by influencing nucleoid structure and dna bending. our results showed that both ihfa and ihf ...201626368515
dickeyafangzhongdai sp. nov., a plant-pathogenic bacterium isolated from pear trees (pyrus pyrifolia).gram-stain-negative, pectinolytic bacteria were repeatedly isolated from pear trees displaying symptoms of bleeding canker in china. three strains, js5t, ln1 and qzh3, had identical 16s rrna gene sequences that shared 99 % similarity to the type strain of dickeya dadantii. phylogenetic analysis of strains js5t, ln1 and qzh3 with isolates representing all species of the genus dickeya and related pectobacterium species supported their affiliation to dickeya. multi-locus sequence typing employing c ...201627045848
survival and growth of cronobacter sakazakii on fresh-cut fruit and the effect of uv-c illumination and electrolyzed water in the reduction of its population.cronobacter sakazakii, found in foods such as powdered infant formula and plant origin ready-to-eat food, is an opportunistic pathogen to infants, neonates and vulnerable adults. the objective of this study was to monitor the growth of c. sakazakii in fresh-cut 'royal gala' apple, 'rocha' pear, and 'piel de sapo' melon, and the effect of uv-c illumination, acidic electrolyzed water (aew) and neutral electrolyzed water (new) in the reduction of its population. fresh-cut fruits were inoculated and ...201627163653
elucidation of enterotoxigenic bacillus cereus outbreaks in austria by complementary epidemiological and microbiological investigations, 2013.identifying bacillus cereus as the causative agent of a foodborne outbreak still poses a challenge. we report on the epidemiological and microbiological investigation of three outbreaks of food poisoning (a, b, and c) in austria in 2013. a total of 44% among 32 hotel guests (a), 22% among 63 employees (b) and 29% among 362 residents of a rehab clinic (c) fell sick immediately after meal consumption. b. cereus isolated from left overs or retained samples from related foods were characterized by t ...201627257745
characterization of virus-derived small interfering rnas in apple stem grooving virus-infected in vitro-cultured pyrus pyrifolia shoot tips in response to high temperature treatment.heat treatment (known as thermotherapy) together with in vitro culture of shoot meristem tips is a commonly used technology to obtain virus-free germplasm for the effective control of virus diseases in fruit trees. rna silencing as an antiviral defense mechanism has been implicated in this process. to understand if high temperature-mediated acceleration of the host antiviral gene silencing system in the meristem tip facilitates virus-derived small interfering rnas (vsirna) accumulation to reduce ...201627716257
integrated analyses using rna-seq data reveal viral genomes, single nucleotide variations, the phylogenetic relationship, and recombination for apple stem grooving virus.next-generation sequencing (ngs) provides many possibilities for plant virology research. in this study, we performed integrated analyses using plant transcriptome data for plant virus identification using apple stem grooving virus (asgv) as an exemplar virus. we used 15 publicly available transcriptome libraries from three different studies, two mrna-seq studies and a small rna-seq study.201627507588
genome sequences of three apple chlorotic leaf spot virus isolates from hawthorns in china.the genome sequences of apple chlorotic leaf spot virus (aclsv) isolates from three accessions of hawthorns (crataegus pinnatifida) grown at shenyang agricultural university were determined using illumina rna-seq. to confirm the assembly data from the de novo sequencing, two aclsv genomic sequences (sy01 and sy02) were sequenced using the sanger method. the sy01 and sy02 sequences obtained with the sanger method showed 99.5% and 99.7% nucleotide identity with the transcriptome data, respectively ...201627519059
identification and characterization of endophytic bacteria isolated from in vitro cultures of peach and pear rootstocks.endophytes are microorganisms which live symbiotically with almost all varieties of plant and in turn helping the plant in a number of ways. instead of satisfactory surface sterilization approaches, repeatedly occurring bacterial growth on in vitro rootstock cultures of peach and pear was identified and isolated as endophytic bacteria in our present study. five different isolates from peach rootstocks were molecularly identified by 16s rrna gene sequencing as brevundimonas diminuta, leifsonia sh ...201628330195
interaction of antifungal peptide bp15 with stemphylium vesicarium, the causal agent of brown spot of pear.peptide bp15 has shown antifungal activity against several plant pathogenic fungi, including stemphylium vesicarium, the causal agent of brown spot of pear. bp15 inhibits the germination, growth and sporulation of s. vesicarium and displays post-infection activity by stopping fungal infection in pear leaves. in this work, live-cell imaging was undertaken to understand the antifungal mechanism of bp15. a double-staining method based on the combination of calcofluor white and sytox green coupled w ...201626693685
physical mapping of black spot disease resistance/susceptibility-related genome regions in japanese pear (pyrus pyrifolia) by bac-fish.black spot disease, caused by alternaria alternata japanese pear pathotype, is one of the most harmful diseases in japanese pear cultivation. in the present study, the locations of black spot disease resistance/susceptibility-related genome regions were studied by fluorescence in situ hybridization using bac clone (bac-fish) on japanese pear (pyrus pyrifolia (burm. f.) nakai) chromosomes. root tips of self-pollinated seedlings of 'osa gold' were used as materials. chromosome samples were prepare ...201627436955
characterization and comparison of the cpk gene family in the apple (malus × domestica) and other rosaceae species and its response to alternaria alternata infection.as one of the ca2+ sensors, calcium-dependent protein kinase (cpk) plays vital roles in immune and stress signaling, growth and development, and hormone responses, etc. recently, the whole genome of apple (malus × domestica), pear (pyrus communis), peach (prunus persica), plum (prunus mume) and strawberry (fragaria vesca) in rosaceae family has been fully sequenced. however, little is known about the cpk gene family in these rosaceae species. in this study, 123 cpk genes were identified from fiv ...201627186637
fine mapping of the gene for susceptibility to black spot disease in japanese pear (pyrus pyrifolia nakai).black spot disease, which is caused by the japanese pear pathotype of the filamentous fungus alternaria alternata (fries) keissler, is one of the most harmful diseases in japanese pear cultivation. we mapped a gene for susceptibility to black spot disease in the japanese pear (pyrus pyrifolia nakai) cultivar 'kinchaku' (aki gene) at the top of linkage group 11, similar to the positions of the susceptibility genes ani in 'osa nijisseiki' and ana in 'nansui'. using synteny-based marker enrichment, ...201627162498
the rna-binding protein csra plays a central role in positively regulating virulence factors in erwinia amylovora.the gacs/gaca two-component system (also called grrs/grra) is a global regulatory system which is highly conserved among gamma-proteobacteria. this system positively regulates non-coding small regulatory rna csrb, which in turn binds to the rna-binding protein csra. however, how gacs/gaca-csr system regulates virulence traits in e. amylovora remains unknown. results from mutant characterization showed that the csrb mutant was hypermotile, produced higher amount of exopolysaccharide amylovoran, a ...201627845410
expression of biphenyl synthase genes and formation of phytoalexin compounds in three fire blight-infected pyrus communis cultivars.pear (pyrus communis) is an economically important fruit crop. drops in yield and even losses of whole plantations are caused by diseases, most importantly fire blight which is triggered by the bacterial pathogen erwinia amylovora. in response to the infection, biphenyls and dibenzofurans are formed as phytoalexins, biosynthesis of which is initiated by biphenyl synthase (bis). two pcbis transcripts were cloned from fire blight-infected leaves and the encoded enzymes were characterized regarding ...201627410389
genome mapping of postzygotic hybrid necrosis in an interspecific pear population.deleterious epistatic interactions in plant inter- and intraspecific hybrids can cause a phenomenon known as hybrid necrosis, characterized by a typical seedling phenotype whose main distinguishing features are dwarfism, tissue necrosis and in some cases lethality. identification of the chromosome regions associated with this type of incompatibility is important not only to increase our understanding of the evolutionary diversification that led to speciation but also for breeding purposes. devel ...201626770810
fuerstia marisgermanicae gen. nov., sp. nov., an unusual member of the phylum planctomycetes from the german wadden sea.members of the phylum planctomycetes are ubiquitous bacteria that dwell in aquatic and terrestrial habitats. while planctomycetal species are important players in the global carbon and nitrogen cycle, this phylum is still undersampled and only few genome sequences are available. here we describe strain nh11(t), a novel planctomycete obtained from a crustacean shell (wadden sea, germany). the phylogenetically closest related cultivated species is gimesia maris, sharing only 87% 16s rrna sequence ...201628066393
hrcu and hrpp are pathogenicity factors in the fire blight pathogen erwinia amylovora required for the type iii secretion of dspa/e.many gram-negative bacterial pathogens mediate host-microbe interactions via utilization of the type iii secretion (t3s) system. the t3s system is a complex molecular machine consisting of more than 20 proteins. collectively, these proteins translocate effectors across extracellular space and into the host cytoplasm. successful translocation requires timely synthesis and allocation of both structural and secreted t3s proteins. based on amino acid conservation in animal pathogenic bacteria, hrcu ...201627206522
identification and expression analysis of genes related to calyx persistence in korla fragrant pear.the objective of this study was to increase understanding about genetic mechanisms affecting calyx persistence in korla fragrant pear (pyrus brestschneideri rehd). flowers were collected at early bloom, full bloom, and late bloom. the rna was extracted from the flowers and then combined according to calyx type. transcriptome and digital gene expression (dge) profiles of flowers, ovaries, and sepals with persistent calyx (sc_hua, sc_ep, and sc_zf, respectively) were compared with those of flowers ...201626911295
potential for using acetic acid plus pear ester combination lures to monitor codling moth in an sit program.studies were conducted in commercial apple orchards in british columbia, canada, to determine whether lures combining ethyl-(e,z)-2,4-decadienoate, pear ester (pe), with either acetic acid (aa) or sex pheromone, (e,e)-8,10-dodecadien-1-ol (codlemone), might improve monitoring of codling moth, cydia pomonella (l.), in an area-wide programme integrating sterile insect technology (sit) and mating disruption (md). catches of sterile and wild codling moths were compared in apple orchards receiving we ...201627898001
control and monitoring of codling moth (lepidoptera: tortricidae) in walnut orchards treated with novel high-load, low-density "meso" dispensers of sex pheromone and pear ester.low-density per ha "meso" dispensers loaded with pear ester, ethyl (e,z)-2,4-decadienoate, kairomone and codlemone, (e,e)-8,10-dodecadien-1-ol, pheromone of codling moth,cydia pomonella(l)., were evaluated versus meso dispensers loaded with pheromone alone for mating disruption control in walnut orchards receiving no insecticide sprays. meso dispensers loaded with codlemone alone (ph meso) were applied at 50 ha(-1)and compared with mesos combining codlemone and pear ester (ph + pe meso) at 25 an ...201627018424
the chemosensory receptors of codling moth cydia pomonella-expression in larvae and adults.olfaction and gustation play critical roles in the life history of insects, mediating vital behaviors such as food, mate and host seeking. chemosensory receptor proteins, including odorant receptors (ors), gustatory receptors (grs) and ionotropic receptors (irs) function to interface the insect with its chemical environment. codling moth, cydia pomonella, is a worldwide pest of apple, pear and walnut, and behavior-modifying semiochemicals are used for environmentally safe control. we produced an ...201627006164
captures of mfo-resistant cydia pomonella adults as affected by lure, crop management system and flight.the main resistance mechanism of codling moth (cydia pomonella) in the tree fruit area of lleida (ne spain) is multifunction oxidases (mfo). we studied the frequency of mfo-resistant adults captured by different lures, with and without pear ester, and flights in orchards under different crop management systems. the factor year affected codling moth mfo-resistance level, particularly in the untreated orchards, highlighting the great influence of codling moth migration on the spread of resistance ...201626497943
theoretical determination of the pk a values of betalamic acid related to the free radical scavenger capacity: comparison between empirical and quantum chemical methods.health benefits of dietary phytochemicals have been suggested in recent years. among 1000s of different compounds, betalains, which occur in vegetables of the cariophyllalae order (cactus pear fruits and red beet), have been considered because of reducing power and potential to affect redox-modulated cellular processes. the antioxidant power of betalains is strictly due to the dissociation rate of the acid moieties present in all the molecules of this family of phytochemicals. experimentally, on ...201626253717
the influence of taste in willingness-to-pay valuations of sirloin steaks from postextraction algal residue-fed cattle.consumer preferences and willingness-to-pay (wtp) for beef sirloin steaks with differing production, physical, and credence attributes related to the use of postextraction algal residue (pear), a novel feed ingredient, were estimated. ninety-six consumers participated in a sensory tasting panel before completing a choice set survey; 127 consumers completed only the choice set survey without sampling products. steaks from grain- and pear-fed steers had similar warner-bratzler shear force (wbsf) s ...201627482694
encapsulating betalains from opuntia ficus-indica fruits by ionic gelation: pigment chemical stability during storage of beads.betalain encapsulation was performed by ionic gelation as a stabilization strategy for these natural pigments. betalains were extracted from purple cactus fruits and encapsulated in calcium-alginate and in combination of calcium alginate and bovine serum albumin. beads were characterised by scanning electron microscopy and thermal analysis using differential scanning calorimetry and thermogravimetry. moisture sorption isotherms were determined. bead morphology was affected by matrix composition. ...201626920307
reproductive system of brown-throated sloth (bradypus variegatus, schinz 1825, pilosa, xenarthra): anatomy and histology.in recent years, habitat degradation led to the decline of some populations of brown-throated sloth. the aim of this study was to describe morphological features of the female reproductive system of the species. the oval ovaries were partially surrounded by ovarian bursa. an external cortex and an inner medulla were present. corpora lutea and corpora albicans together with follicles at various stages of development each with a single oocyte were found in the cortex. uterine tubes were tortuous, ...201626250652
a proposal of linear assessment scheme for the udder of dairy camels (camelus dromedarius l.).digital images from the left side of the mammary gland of 146 multiparous lactating dromedary camels in mid lactation and managed under intensive conditions were obtained immediately before milking and used to build up a reference scheme for the morphological evaluation of camel mammary glands. a 5-point linear scoring scheme (with 0.5-point accuracy) was subsequently generated based on five mammary traits (udder, depth and floor inclination, teats, shape, length, and width). results showed that ...201627038610
morphological and molecular characterization of myxobolus sheyangensis n. sp. (myxosporea: myxobolidae) with intralamellar sporulation in allogynogenetic gibel carp, carassius auratus gibelio (bloch) in china.allogynogenetic gibel carp is one of the most important freshwater cultured species in china. however, myxosporidiosis represents a severe threat to prevent the sustainable development of aquaculture of this species. during the investigation of myxosporean diversity of reared allogynogenetic gibel carp in east china, a new myxosporean with typical characteristics of myxobolus was found from 169 out of 210 (80.4 %) examined samples, designated as myxobolus sheyangensis n. sp. by combing comparati ...201627225002
intra-oral acid production associated with eating whole or pulped raw fruits.the hypotheses that raw fruits, whether whole or pulped, were cleared rapidly from the mouth and that the sugars in the whole and pulped fruits are fermented with equal efficiency to acids by the oral microflora were tested in this study. groups of 7-9 adult subjects chewed 10 g of raw, whole or pulped fruit (apple, banana, orange, pear and pineapple) for 1 min and whole, unstimulated saliva samples were collected during the following 60-min interval. each saliva sample was assayed for the conce ...201615181333
comparative genomic analysis of the grf genes in chinese pear (pyrus bretschneideri rehd), poplar (populous), grape (vitis vinifera), arabidopsis and rice (oryza sativa).growth-regulating factors (grfs) are plant-specific transcription factors that have important functions in regulating plant growth and development. previous studies on grf family members focused either on a single or a small set of genes. here, a comparative genomic analysis of the grf gene family was performed in poplar (a model tree species), arabidopsis (a model plant for annual herbaceous dicots), grape (one model plant for perennial dicots), rice (a model plant for monocots) and chinese pea ...201627933074
promotion of flowering by apple latent spherical virus vector and virus elimination at high temperature allow accelerated breeding of apple and pear.plant viral vectors are superior tools for genetic manipulation, allowing rapid induction or suppression of expression of a target gene in plants. this is a particularly effective technology for use in breeding fruit trees, which are difficult to manipulate using recombinant dna technologies. we reported previously that if apple seed embryos (cotyledons) are infected with an apple latent spherical virus (alsv) vector (alsv-atft/mdtfl1) concurrently expressing the arabidopsis thaliana florigen (a ...201626941750
pectic polysaccharides are attacked by hydroxyl radicals in ripening fruit: evidence from a fluorescent fingerprinting method.many fruits soften during ripening, which is important commercially and in rendering the fruit attractive to seed-dispersing animals. cell-wall polysaccharide hydrolases may contribute to softening, but sometimes appear to be absent. an alternative hypothesis is that hydroxyl radicals ((•)oh) non-enzymically cleave wall polysaccharides. we evaluated this hypothesis by using a new fluorescent labelling procedure to 'fingerprint' (•)oh-attacked polysaccharides.201626865506
effect of ultrasound on survival and growth of escherichia coli in cactus pear juice during storage.the aim of this study was to investigate the effectiveness of ultrasound as a conservation method for the inactivation of escherichia coli inoculated into cactus pear juices (green and purple). total soluble solids, ph, titratable acidity, and the kinetics of e. coli in cactus pear juices treated by ultrasound (60%, 70%, 80% and 90% amplitude levels for 1, 3 and 5min) were evaluated over 5 days. total inactivation was observed in both fruit juices after 5min of ultrasound treatment at most ampli ...201626991288
iron-modulated pseudocyst formation in tritrichomonas foetus.iron is an essential element for the survival of trichomonads during host-parasite interaction. the availability of this metal modulates several metabolic pathways of the parasites and regulates the expression of virulence factors such as adhesins and proteolytic enzymes. in this study, we investigated the effect of iron depletion on the morphology and life cycle of tritrichomonas foetus. scanning and transmission electron microscopy analyses revealed that depletion of iron from the culture medi ...201627253439
xylella taiwanensis sp. nov., causing pear leaf scorch disease.a gram-stain-negative, nutritionally fastidious bacterium (pls229t) causing pear leaf scorch was identified in taiwan and previously grouped into xylella fastidiosa. yet, significant variations between pls229t and xylellafastidiosa were noted. in this study, pls229t was evaluated phenotypically and genotypically against representative strains of xylellafastidiosa, including strains of the currently known subspecies of xylellafastidiosa, xylella fastidiosa subsp. multiplex and 'xylella fastidiosa ...201627530392
novel approach of using nutraceutic-directed caloric antioxidant density and ion-ratio for evaluating fruit's health quality.seven kinds of indigenous fruits and five imported fruits were compared for their "health quality." methods including the calorific, antioxidant, and ion ratios were carried out. results indicated the order of content (in mg/100g) was: ca(2+) , murcott orange (218.2) > kiwifruit (200.0) > pineapple (138.5) > golden kiwi (117.6); mg(2+) , pitaya (192.2), banana (88.0), kiwifruit (63.4), and golden kiwi (58.4); zn(2+) , pitaya (19.53) > pear (10.8) > kiwifruit (6.09) > irwin mango (4.58). cu(2+) , ...201627467811
population genetic analyses of the fungal pathogen colletotrichum fructicola on tea-oil trees in china.the filamentous fungus colletotrichum fructicola is found in all five continents and is capable of causing severe diseases in a number of economically important plants such as avocado, fig, cocoa, pear, and tea-oil trees. however, almost nothing is known about its patterns of genetic variation and epidemiology on any of its host plant species. here we analyzed 167 isolates of c. fructicola obtained from the leaves of tea-oil tree camellia oleifera at 15 plantations in seven chinese provinces. mu ...201627299731
predicting the dynamic impact behaviour of spray droplets on flat plant surfaces.the dynamic impact behaviour of water droplets on plant surfaces was investigated based on a multiphase computational fluid dynamics (cfd) model. the study was conducted using the volume of fluid (vof) approach. the static contact angle of water droplets on leaf surfaces of different plants (apple, pear, leek and cabbage) was measured and found to vary between 54.9 and 138.2°. impact experiments were conducted by monitoring the flow and impact characteristics of water droplets on leaves in still ...201627501228
traditional chinese medicine, food therapy, and hypertension control: a narrative review of chinese literature.despite the lack of english literature about traditional chinese medicine (tcm) food therapy, there is abundant chinese literature about the application of food therapy for hypertension control. this paper summarizes basic concepts of tcm, the principles of food therapy and its application for hypertension control according to chinese literature. in tcm, food is conceptualized according to both nutritional and functional aspects, and can be used to treat illnesses. four principles of tcm food th ...201627852126
three new and a known species of the genus <i>proleptonchus</i> lordello, 1955 (nematoda: leptonchidae) with a diagnostic compendium of the genus.this paper deals with the description of three new and one known species of the genus proleptonchus lordello, 1955. proleptonchus kazirangus n. sp. from kaziranga national park, india is characterized by having 1.08-1.45 mm long body; cap-like, offset, lip region; 7-8 µm long odontostyle and 10-12 µm long odontophore; short, pear-shaped pharyngeal bulb offset by a constriction, occupying about 18-21% of total neck length; female genital system mono-prodelphic; pars dilatata uteri with distinctly ...201627988759
migrating zooids allow the dispersal of fredericella sultana (bryozoa) to escape from unfavourable conditions and further spreading of tetracapsuloides bryosalmonae.fredericella sultana (bryozoa: phylactolaemata) is a primary host in the two-host life cycle of the myxozoan parasite tetracapsuloides bryosalmonae, the etiological agent of proliferative kidney disease (pkd) in salmonids. overtly infected f. sultana colonies were collected from river kamp (lower austria), following the first pkd outbreak affecting autochthonous brown trout (salmo trutta) in austria. zooids cultured under unfavourable conditions, e.g. hypertrophication or sudden temperature chan ...201627546864
genome-wide identification and comparative analysis of the cation proton antiporters family in pear and four other rosaceae species.the monovalent cation proton antiporters (cpas) play essential roles in plant nutrition, development, and signal transduction by regulating ion and ph homeostasis of the cell. the cpas of plants include the na(+)/h(+) exchanger, k(+) efflux antiporter, and cation/h(+) exchanger families. however, currently, little is known about the cpa genes in rosaceae species. in this study, 220 cpa genes were identified from five rosaceae species (pyrus bretschneideri, malus domestica, prunus persica, fragar ...201627193473
studies of the macroscopic and microscopic morphology (hippocampus) of brain in vencobb broiler.the aim of this study was to study the anatomy of different parts of brain and histology of hippocampus of vencobb broiler chicken.201627284228
distribution of α-transducin and α-gustducin immunoreactive cells in the chicken (gallus domesticus) gastrointestinal tract.the expression and distribution patterns of the taste signaling molecules, α-gustducin (gαgust) and α-transducin (gαtran) g-protein subunits, were studied in the gastrointestinal tract of the chicken (gallus domesticus) using the immunohistochemical method. gαgust and gαtran immunoreactive (-ir) cells were observed in the mucosal layer of all examined segments, except the esophagus, crop, and the saccus cranialis of the gizzard. the highest numbers of gαgust and gαtran-ir cells were found in the ...201626957624
thorectinae (porifera: demospongiae: dictyoceratida) from northeastern brazil: two new species and transfer of scalarispongia cincta (boury-esnault, 1973) to the genus thorecta lendenfeld, 1888.the genus scalarispongia was raised to gather species formerly allocated in cacospongia with rectangular fibrous skeleton of cored primary fibers and uncored secondary fibers. species of the genus thorecta have a similar skeletal architecture, but the presence of a dermal armour distinguishes them from scalarispongia. here we describe two new species of scalarispongia from northeastern brazil and transfer scalarispongia cincta to thorecta cincta. scalarispongia tubulata sp. nov. is thick encrust ...201627811660
further studies on south eastern asian luciolinae: 1. sclerotia ballantyne, <br />a new genus of fireflies with back swimming larvae 2. triangulara pimpasalee, a new genus from thailand (coleoptera: lampyridae).sclerotia ballantyne gen. nov. is established for seven species. sclerotia aquatilis (thancharoen, 2007), scl. brahmina (bourgeois, 1890), scl. carinata (gorham, 1880), scl. flavida (hope, 1845), scl. seriata (olivier, 1891), and scl. substriata (gorham, 1880) are transferred from luciola laporte. sclerotia fui sp. nov. from china is described as new. luciola cingulata olivier, 1885 is synonymised with luciola substriata gorham. luciola formosana pic, 1916 is removed from synonymy with luciola s ...201627701260
two new species of chromadoridae (chromadorida: nematoda) from the east china sea.two new species of chromadoridae, a family of free-living marine nematodes, are described from intertidal sediments of the east china sea. ptycholaimellus pirus sp. nov. is characterized by having a cuticle with six longitudinal rows of double dots and long somatic setae, relatively long cephalic setae, a pear-shaped terminal pharyngeal bulb occupying less than 30% of pharyngeal length, and an elongate conical tail. the new species is distinguished from all related species by the unique characte ...201627470838
a revision of the genus macrolygistopterus pic, 1929 (coleoptera, lycidae, calochromini).a taxonomic review of the lycid genus macrolygistopterus pic, 1929 with illustrations of diagnostic characters, geographic distribution maps and an identification key to the species is presented. also, a key to the world calochromini is given. of the 12 species of the genus, 9 were studied: m. succinctus (latreille, 1811), m. quadricostatus (buquet, 1842), m. caeruleus (gorham, 1884), m. germaini pic, 1930, m. grandjeani pic, 1930, m. subparallelus pic, 1930, m. testaceirostris pic, 1930, m. sim ...201627394782
hyphomonas pacifica sp. nov., isolated from deep sea of the pacific ocean.three gram-negative, aerobic, non-spore-forming, oval- to pear-shaped bacterial strains (t16b2(t), t24b3, and c76ad) were isolated from petroleum-degrading microbial communities through an enrichment of sediments and seawater samples from the pacific ocean. phylogenetic analysis showed strains t16b2(t), t24b3, and c76ad to form a robust clade together with hyphomonas atlanticus 22ii1-22f38(t) and hyphomonas beringensis 25b14_1(t) (16s rrna identity ≥99.6 %). genomic average nucleotide identity a ...201627255138
botrytis pyriformis sp. nov., a novel and likely saprophytic species of botrytis.a novel species of botrytis from sedum sarmentosum was described based on morphology and analyses of dna sequences of nuc rdna its regions and three nuclear genes (g3pdh, hsp60, rpb2). meanwhile pathogenicity in 32 plant species, response to temperature for growth and conidial germination for the species were determined. the botrytis species was named botrytis pyriformis sp. nov. it was characterized by formation of grayish mycelia, brownish conidia and melanized sclerotia on pda. the conidia ar ...201627153884
complex processing of prickly pear cactus (opuntia sp.) by free-ranging long-tailed macaques: preliminary analysis for hierarchical organisation.complex food-processing techniques by gorillas, chimpanzees, and orangutans have allowed comparisons of complex hierarchical cognition between great apes and humans. here, we analyse preliminary observations of free-ranging long-tailed macaques (macaca fascicularis) (n = 3) in thailand processing opuntia sp. cactus fruits. from our observations, we suggest that there is potential to extend the analyses of hierarchical cognition to old world monkeys. we found that the macaques used six behavioura ...201626921023
pregnancy complicated by gorham-stout disease and refractory chylothorax.introduction gorham-stout disease (gsd) is a rare disorder of bony destruction due to lymphangiomatosis, and is often triggered by hormones. one complication of gsd is the development of chylothorax, which carries a high mortality rate. very little experience has been published to guide management in gsd during pregnancy to optimize both fetal and maternal health. case study a 20-year-old woman with known gsd presented with shortness of breath at 18 weeks of pregnancy, due to bilateral chylothor ...201627708981
epilachnini (coleoptera: coccinellidae)-a revision of the world genera.based on the recent revised generic classification of the tribe epilachnini (szawaryn et al. 2015), all 27 genera are re-described, diagnosed, illustrated, and included in an identification key. the following nomenclatural changes are made: epilachna (hypsa) mulsant 1850, epilachna (cleta) mulsant 1850, solanophila weise 1898, epilachna (aparodentata) wang and cao 1993, and epilachna (uniparodentata) wang and cao 1993 are removed from synonymy of epilachna chervolat 1837. the subgenus cleta of e ...201627651424
nonmalignant adult thoracic lymphatic disorders.the thoracic lymphatic disorders are a heterogeneous group of uncommon conditions that are associated with thoracic masses, interstitial pulmonary infiltrates, and chylous complications. accurate diagnosis of the thoracic lymphatic disorders has important implications for the newest approaches to management, including embolization and treatment with antilymphangiogenic drugs. new imaging techniques to characterize lymphatic flow, such as dynamic contrast-enhanced magnetic resonance lymphangiogra ...201627514588
proverb comprehension in individuals with agenesis of the corpus callosum.comprehension of non-literal language involves multiple neural systems likely involving callosal connections. we describe proverb comprehension impairments in individuals with isolated agenesis of the corpus callosum (agcc) and normal-range general intelligence. experiment 1 compared gorham proverb test (gorham, 1956) performance in 19 adults with agcc and 33 neurotypical control participants of similar age, sex, and intelligence. experiment 2 used the proverbs subtest of the delis-kaplan execut ...201627448531
case report: gorham-stoute syndrome with involvement of majority of mandible, and partial maxillary, temporal and zygomatic bones.the gorham-stout syndrome is a rare condition in which spontaneous, progressive resorption of bone occurs. the diagnosis is based on clinical, radiological and histopathological findings after excluding other etiologies and as a result it is often delayed. we present the case of a 21-year-old male diagnosed with gorham-stout syndrome with involvement of the majority of the mandible, partial involvement of right maxillary, temporal and zygomatic bones and discuss its diagnostic and therapeutic fe ...201627408465
effect of sunlight-exposure on antioxidants and antioxidant enzyme activities in 'd'anjou' pear in relation to superficial scald development.influence of preharvest sunlight exposure on superficial scald development in 'd'anjou' pears during cold storage was investigated. the biochemical changes related to scald including α-farnesene, conjugated trienols (ctols), antioxidants, antioxidant enzyme activities were monitored among separated blushed and shaded peels of unbagged fruit as well as the whole peel of bagged fruit. in unbagged fruit, scald symptom was restricted to shaded peel; while there was no difference in α-farnesene betwe ...201627211615
erratum: pregnancy complicated by gorham-stout disease and refractory chylothorax.[this corrects the article doi: 10.1055/s-0036-1593443.].201627822433
painful sporadic osteolysis of the parietal bone 'gorham's disease'.we report a 62-year old man, with painful osteolysis 'gorham disease' of skull. to ameliorate pain, large craniotomy and cranioplasty was performed. there was no sign of recurrence after one year. a brief review of the literature is presented.201627331246
cystic angiomatosis, a heterogeneous condition: four new cases and a literature review.cystic angiomatosis (ca) is a rare disorder causing bony cysts. it displays some similarity to gorham-stout disease (gsd), but has a much better local prognosis, despite the larger number of cysts. these 2 conditions also differ in terms of their location, visceral involvement, and response to treatment.201627787381
18f-naf and 18f-fdg pet/ct in gorham-stout disease.gorham-stout disease (gsd) is an extremely rare skeletal disorder of unknown etiology characterized by benign proliferation of vascular or lymphatic channels, leading to progressive bone resorption. we report on a patient diagnosed with gsd affecting the right scapula and the right ribs, who underwent pet/ct scans using f-fdg and f-naf. the remnant upper portion of the affected scapula did not show f-fdg uptake but demonstrated markedly increased f-naf activity. furthermore, intense f-naf activi ...201627648707
a review of the <i>tillicera</i> genus group with a revision of <i>plathanocera</i> schenkling (coleoptera, cleridae, clerinae).the tillicera genus group is revised and defined to contain 40 species in the following six genera: apopempsis schenkling, 1903; cardiostichus quedenfeldt, 1885; hemitrachys gorham, 1876; placocerus klug, 1837; plathanocera schenkling, 1902 and tillicera spinola, 1941. the definition of the tillicera genus group is mainly based on the presence of the dorsoventrally compressed and expanded antennae (from antennomere 3 onwards). the antennomeres are dilated at least from antennomeres 5 to 9 onward ...201627988691
sirolimus on gorham-stout disease. case report.gorham-stout disease (gsd) is a rare disease of unknown etiology characterized by vascular proliferation that produces destruction of bone matrix.201628348444
multicentric disappearing bone disease treated with arthroplasty.multicentric disappearing bone disease, or gorham disease, is a rare entity. a middle age woman, presented to us with left sided antalgic gait and severe bony deformity of her left knee. radiograph revealed massive bone defect of the medial condyle of the left tibia with subluxation of the knee joint. she was scheduled for knee replacement in six months. however, she developed another lesion over the right hip that typically mimicked the disease progression of disappearing bone disease. the righ ...201628553448
automatic analysis of the 3-d microstructure of fruit parenchyma tissue using x-ray micro-ct explains differences in aeration.3d high-resolution x-ray imaging methods have emerged over the last years for visualising the anatomy of tissue samples without substantial sample preparation. quantitative analysis of cells and intercellular spaces in these images has, however, been difficult and was largely based on manual image processing. we present here an automated procedure for processing high-resolution x-ray images of parenchyma tissues of apple (malus × domestica borkh.) and pear (pyrus communis l.) as a rapid objectiv ...201526518365
molecular characterization and expression analyses of an anthocyanin synthase gene from magnolia sprengeri pamp.anthocyanin synthase (ans), which catalyzes the conversion of colorless leucoanthocyanins into colored anthocyanins, is a key enzyme in the anthocyanin biosynthetic pathway. it plays important roles in plant development and defense. an ans gene designated as msans was cloned from magnolia sprengeri using rapid amplification of complementary dna (cdna) ends technology. the full-length msans is 1171-bp long and contains a 1080-bp open reading frame encoding a 360 amino acid polypeptide. in a seque ...201525315387
fate of alicyclobacillus spp. in enrichment broth and in juice concentrates.in this study, the fate of alicyclobacillus acidoterrestris spores in different types of juice concentrates stored under different conditions was investigated. in addition, the impact of dilution procedures during the enrichment step for the detection of alicyclobacillus in lemon juice concentrates was studied. pear, red grape, mango, tangerine, carrot and lemon juice concentrates (50-69.4°brix, ph1.7-4.3) were inoculated with a. acidoterrestris spores (10(3) spore/ml) and stored at 4 °c and 20 ...201526102554
unifying bacteria from decaying wood with various ubiquitous gibbsiella species as g. acetica sp. nov. based on nucleotide sequence similarities and their acetic acid secretion.bacteria were isolated from necrotic apple and pear tree tissue and from dead wood in germany and austria as well as from pear tree exudate in china. they were selected for growth at 37 °c, screened for levan production and then characterized as gram-negative, facultatively anaerobic rods. nucleotide sequences from 16s rrna genes, the housekeeping genes dnaj, gyrb, reca and rpob alignments, blast searches and phenotypic data confirmed by maldi-tof analysis showed that these bacteria belong to th ...201526071988
planctomicrobium piriforme gen. nov., sp. nov., a stalked planctomycete from a littoral wetland of a boreal lake.an aerobic, budding, non-pigmented and rosette-forming bacterium was isolated from a littoral wetland of a boreal lake located in valaam island, northern russia, and designated strain p3(t). ellipsoidal to pear-shaped cells of this bacterium were covered with crateriform pits and possessed stalks suggesting a planctomycete morphotype. 16s rrna gene sequence analysis confirmed that strain p3(t) was a member of the order planctomycetales and belonged to a phylogenetic lineage defined by the genus ...201525724745
species-specific duplications driving the recent expansion of nbs-lrr genes in five rosaceae species.disease resistance (r) genes from different rosaceae species have been identified by map-based cloning for resistance breeding. however, there are few reports describing the pattern of r-gene evolution in rosaceae species because several rosaceae genome sequences have only recently become available.201525759136
genome-wide identification and comparative analysis of the heat shock transcription factor family in chinese white pear (pyrus bretschneideri) and five other rosaceae species.heat shock transcription factors (hsfs), which act as important transcriptional regulatory proteins in eukaryotes, play a central role in controlling the expression of heat-responsive genes. at present, the genomes of chinese white pear ('dangshansuli') and five other rosaceae fruit crops have been fully sequenced. however, information about the hsfs gene family in these rosaceae species is limited, and the evolutionary history of the hsfs gene family also remains unresolved.201525604453
oxidative stress associated with rootstock-scion interactions in pear/quince combinations during early stages of graft development.exposing a plant to stress situations, such as grafting, generally triggers antioxidant defense systems. in fruit tree grafting, quince (cydonia oblonga) is widely used as a rootstock for pear (pyrus communis l.), but several economically important pear cultivars are incompatible with available quince rootstocks. in this study, grafts were established using an in vitro callus graft system mimicking the events taking place in fruit trees. in vitro grown callus from pear [p. communis l. cv. 'confe ...201525543953
landscape ethnoecological knowledge base and management of ecosystem services in a székely-hungarian pre-capitalistic village system (transylvania, romania).previous studies showed an in-depth ecological understanding by traditional people of managing natural resources. we studied the landscape ethnoecological knowledge (leek) of székelys on the basis of 16-19(th) century village laws. we analyzed the habitat types, ecosystem services and sustainable management types on which village laws had focused.201525567233
agave as a model cam crop system for a warming and drying world.as climate change leads to drier and warmer conditions in semi-arid regions, growing resource-intensive c3 and c4 crops will become more challenging. such crops will be subjected to increased frequency and intensity of drought and heat stress. however, agaves, even more than pineapple (ananas comosus) and prickly pear (opuntia ficus-indica and related species), typify highly productive plants that will respond favorably to global warming, both in natural and cultivated settings. with nearly 200 ...201526442005
changes in the structural organization of the cytoskeleton of tritrichomonas foetus during trophozoite-pseudocyst transformation.tritrichomonas foetus is a parasite that causes bovine trichomonosis, a major sexually transmitted disease in cattle. it grows in axenic media as a trophozoite with a pear-shaped body, three anterior flagella, and one recurrent flagellum. however, under some well-controlled experimental conditions in vitro, as well as in vivo in infected bulls, the parasite acquires a spherical or elliptical shape, and the flagella are internalized but the cells do not display a cyst wall. this form, known as th ...201525880467
genome assembly of schlumbergera virus x infecting prickly pear (opuntia cochenillifera) in brazil.potexviruses frequently infect plants from the family cactaceae. we report the complete genome sequence of a new schlumbergera virus x (schvx) isolate. the genome has 6,615 nucleotides and shares 94% nucleotide identity with schvx-k11 from schlumbergera. this is the first sequence of an isolate of schvx from the genus opuntia.201525792054
occupational allergy to peach (prunus persica) tree pollen and potential cross-reactivity between rosaceae family pollens.orchard workers in north china are highly exposed to orchard pollens, especially peach and other rosaceae family pollens during pollination season. the aim of this study was to investigate whether occupational allergy to peach tree pollen as a member of rosaceae family is ige-mediated and to evaluate the cross-reactivity among rosaceae family pollens. allergen skin test and conjunctival challenge test were performed; enzyme linked immune-sorbent assay (elisa), inhibiting elisa, western immunoblo ...201526742437
interaction of polyphenol oxidase of solanum tuberosum with β-cyclodextrin: process details and applications.polysaccharides differing in structure and chemical nature were screened for their ability to bind non-covalently with polyphenol oxidase (ppo) from potato (as a model) and their effect on enzyme activity. all the polysaccharides selected inhibited the ppo but β-cyclodextrin showed maximum inhibition under optimum conditions. process details for the inhibition of ppo were studied with respect to concentration of β-cyclodextrin, temperature, ph, and time. higher inhibition constant and lower half ...201526187193
patulin and its dietary intake by fruit juice consumption in iran.a survey was conducted to determine levels and dietary intake of patulin (pat) from fruit juices consumed in iran. pat content was determined using high performance liquid chromatography-diode array detection in 161 samples of fruit juices including apple, pineapple, pear, peach, pomegranate and white and red grape juices. results revealed the presence of pat in 16.1% of the samples examined, ranging from 5 to 190.7 µg/kg. average and median pat content in positive samples was 34.5 and 18.5 µg/k ...201525494337
traditional beliefs and practices among mexican american immigrants with type ii diabetes: a case study.to describe selected common health beliefs and practices among mexican american immigrants with type ii diabetes.201525156743
tes, licuados, and capsulas: herbal self-care remedies of latino/hispanic immigrants for type 2 diabetes.the purpose of this cross-sectional, descriptive study was to explore the characteristics of herbal remedy use for diabetes among latinos/hispanics with type 2 diabetes.201524030377
betalains, phenols and antioxidant capacity in cactus pear [opuntia ficus-indica (l.) mill.] fruits from apulia (south italy) genotypes.betacyanin (betanin), total phenolics, vitamin c and antioxidant capacity (by trolox-equivalent antioxidant capacity (teac) and oxygen radical absorbance capacity (orac) assays) were investigated in two differently colored cactus pear (opuntia ficus-indica (l.) mill.) genotypes, one with purple fruit and the other with orange fruit, from the salento area, in apulia (south italy). in order to quantitate betanin in cactus pear fruit extracts (which is difficult by hplc because of the presence of t ...201526783704
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