Publications

sequence and structure of defective interfering rnas associated with cucumber necrosis virus infections.	the presence of symptom-attenuating defective interfering (di) rnas in a laboratory culture of cucumber necrosis tombusvirus (cnv) was confirmed. sequencing of cdna clones of these di rnas revealed that cnv di rnas retained sequences from the cnv 5'-untranslated and 3'-terminal regions as well as a portion of the coding region for the 92k protein. similar sequence arrangements were also observed in symptom-attenuating di rnas generated de novo from synthetic wild-type cnv transcripts. a comparis ...	1993	8345363
symptomatology and movement of a cucumber necrosis virus mutant lacking the coat protein protruding domain.	a cucumber necrosis virus (cnv) mutant which lacks the coding sequence for the coat protein protruding domain, pd(-), was constructed by site-directed mutagenesis of an infectious cnv cdna clone, pk2/m5 (wild-type, 4701 nt). transcripts of pd(-) were infectious on nicotiana clevelandii; however, local lesions produced were significantly smaller than those on the corresponding leaves of plants inoculated with wild-type transcript. in addition, systemic symptoms took 8 to 12 days longer to develop ...	1993	8460495
rapid de novo generation of defective interfering rna by cucumber necrosis virus mutants that do not express the 20-kda nonstructural protein.	it is generally believed that serial passage at high multiplicity of infection (moi) is required for the generation of defective interfering (di) particles. high levels of di rnas are found associated with persistent infections initiated with laboratory cultures of cucumber necrosis virus (cnv). two synthetic cnv transcripts that were derived through site-directed mutagenesis of a highly infectious cnv cdna clone and that do not express the cnv 20-kda nonstructural protein were found to generate ...	1991	1722320
the complete nucleotide sequence of tobacco necrosis virus strain d.	the complete sequence of the rna genome of tobacco necrosis virus strain d (tnv-d) consisting of 3759 nucleotides has been determined. the positive strand contains five open reading frames (orfs). the 5'-proximal orf encodes a 22k protein terminating with an amber codon which may be read through to produce a 82k protein (p82). two small centrally located orfs each encode two out-of-frame 7k proteins (p7a and p7b). the 3'-proximal orf encodes the 29k coat protein (cp), the n terminus of which has ...	1991	1856691
infectious transcripts from cloned cucumber necrosis virus cdna: evidence for a bifunctional subgenomic mrna.	highly infectious synthetic transcripts from full-length cucumber necrosis virus (cnv) cdna clones have been prepared. infections produced by these transcripts, by cnv rna, and by mutated transcripts are compared. inoculation with natural cnv rna resulted in a mild systemic necrosis in nicotiana clevelandii, whereas inoculation with synthetic cnv transcripts resulted in severe systemic necrosis. an abundant low molecular weight rna species was associated with cnv rna infections but was not detec ...	1991	2014641
the effect of flurbiprofen on herpes simplex virus type 1 stromal keratitis in mice.	the use of steroidal compounds to reduce the inflammation and scarring associated with herpes simplex virus type 1 (hsv-1) stromal keratitis can result in severe exacerbation of the corneal disease. we compared the nonsteroidal anti-inflammatory drug (nsaid) flurbiprofen sodium with dexamethasone for the treatment of hsv-1 induced corneal stromal disease in an inbred mouse model. stromal disease was induced by the direct intrastromal injection of hsv-1. a stromal opacity and corneal neovasculari ...	1990	2167299
genome structure of tobacco necrosis virus strain a.	an almost complete sequence of the rna genome of tobacco necrosis virus (tnv) strain a has been determined. the genome organization is very similar to that of carnation mottle virus (carmv) and turnip crinkle virus (tcv). the 5'-proximal open reading frame (orf) encodes a 23-kda protein and read-through of its amber codon into the second orf is presumably used for the translation of a 82-kda protein. the third large orf encodes the 30-kda coat protein. two small orfs are located upstream and one ...	1990	2371773
translation of cucumber necrosis virus rna in vitro.	the in vitro translation products directed by cucumber necrosis virus (cnv) rna were analysed in both rabbit reticulocyte lysate and wheatgerm extract cell-free translation systems. in rabbit reticulocyte lysates, one major protein of approximate mr 34.6k was produced. in wheatgerm extracts, four proteins of approximate mr values 41.6k, 34.6k, 24k and 20k were produced. the genomic locations of the cnv in vitro translation products were determined using several experimental approaches including, ...	1990	2230730
organization of tomato bushy stunt virus genome: characterization of the coat protein gene and the 3' terminus.	we have synthesized cdna clones of the genome of the cherry strain of tomato bushy stunt virus (tbsv-cherry) and have used them as hybridization probes to identify and position two 3' coterminal subgenomic rnas of approximately 2.2 and 0.9 kilobases (kb) in length. the 5' termini of the two subgenomic rnas have been mapped to positions located 2156 and 936 nucleotides respectively from the 3' terminus of the viral genome. the nucleotide sequence of cdna clones encompassing the region of the geno ...	1989	2922927
cdna cloning of artichoke mottled crinkle virus rna and localization and sequencing of the coat protein gene.	we report the cdna cloning of the genomic rna of artichoke mottled crinkle virus (amcv), which is a member of tombusvirus group. amcv has a monopartite positive sense rna genome, which is not polyadenylated at the 3' end. the genome size is 4.8 kb. we have localized and sequenced the open reading frame (orf) encoding the coat protein. unlike most monopartite positive-strand rna plant viruses, the orf is not located near the 3' end, but like other members of the tombusvirus group, cyrsv (cymbidiu ...	1989	2491684
complete nucleotide sequence of the cucumber necrosis virus genome.	the complete nucleotide sequence of the cucumber necrosis virus (cnv) genome has been determined. the genome is 4701 nucleotides in length and contains five long open reading frames (orf). orf1 begins at the first aug codon at the 5' terminus and terminates at an amber codon. the predicted molecular weight of the polyprotein encoded by orf1 is 33 kilodaltons (kda). readthrough of the orf1 amber codon would yield a protein with a molecular weight of 92 kda. comparison of the amino acid sequence o ...	1989	2705296
clinical neurophysiology of dementia.	the role of eeg in the study of the dementias is to help in the differential diagnosis of the multiple causes of this syndrome. eeg is useful in differentiating early on between treatable and as of now untreatable forms of dementia. space-occupying lesions that give rise to dementia are reliably detected by eeg. infectious, toxic, and metabolic processes are associated with early and severe electroencephalographic abnormalities. the "slow virus" infections show characteristic electrical patterns ...	1986	2940455
purification and properties of cucumber necrosis virus and a smaller top component.		1972	4623504
the relationship between cucumber necrosis virus and its vector, olpidium cucurbitacearum.		1970	4990057
transmission of cucumber necrosis virus by olpidium cucurbitacearum barr & dias.		1970	5450390