Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| evaluation of the antimicrobial activity of a new super-oxidized water, sterilox, for the disinfection of endoscopes. | the antimicrobial activity of a new super-oxidized water, sterilox, has been tested against mycobacterium tuberculosis, mycobacterium avium-intracellulare, mycobacterium chelonae, escherichia coli (including type o157), enterococcus faecalis, pseudomonas aeruginosa, bacillus subtilis var niger spores, methicillin-resistant staphylococcus aureus, candida albicans, poliovirus type 2 and human immunodeficiency virus hiv-1. under clean conditions, freshly generated sterilox was found to be highly ac ... | 1999 | 9949966 |
| oral wart associated with human papillomavirus type 2. | more than 100 human papillomavirus (hpv) types have been identified to date. of these, 24 types have been described as being associated with oral lesions. hpv-2 has been frequently associated with skin lesions, but the reports of oral lesions as features of mucosal infection are limited. a biopsy specimen of an oral wart on the right palate was taken from a 48-year-old man and examined for the presence of hpv the sections showed papillary growth of the epithelium with hyperkeratosis and parakera ... | 1999 | 10069543 |
| paralytic poliomyelitis associated with live oral poliomyelitis vaccine in child with hiv infection in zimbabwe: case report. | to describe a complication of oral vaccination with live, attenuated poliomyelitis virus in a child infected with hiv. | 1999 | 10092261 |
| serum antibodies to polioviruses in alexandria, egypt. | random sera, in a total of 192, were collected in the fever hospital of alexandria, egypt, and analysed for the presence of antibodies against polioviruses. the results show good antibody levels, only three sera (1.5%) were negative for poliovirus type 1, 5 (2.6%) for poliovirus type 2 and 10 (5.2%) for poliovirus type 3; one subject was completely negative. | 1999 | 10395057 |
| antiviral activity of south brazilian medicinal plant extracts. | brazilian plants are potential sources of useful edible and medicinal plants. hydromethanolic extracts prepared from 54 medicinal plants used in folk medicine to treat infections were screened for antiviral properties against five different viruses (hsv-1, hsv-2, poliovirus type 2, adenovirus type 2 and vsv). fifty-two percent of the plant extracts exhibited antiviral against one or more tested viruses. more specifically, 42.6% showed activity against hsv-1 (herpes simplex virus type 1), 42.6% a ... | 1999 | 10439486 |
| 'primer alignment-and-extension': a novel mechanism of viral rna recombination responsible for the rescue of inactivated poliovirus cdna clones. | in the course of experiments designed to assess the potential role of alternative open reading frames (orf) present in the 5'-terminal untranslated region (5'-utr) of poliovirus type 1 (mahoney strain) genomic rna, we came across a double mutation that completely abrogated the infectivity of full-length cdna clones. the infectivity was rescued in trans by cotransfecting cos-1 cells with short rna transcripts of the wild-type 5'-utr of poliovirus type 2 lansing, provided a free 3'-oh was availabl ... | 1999 | 10466784 |
| progress toward the global interruption of wild poliovirus type 2 transmission, 1999. | since 1988, when the world health assembly resolved to eradicate poliomyelitis globally by 2000, substantial progress has been made in attaining this goal: the americas, the pacific rim, europe, and central asia appear to be polio-free. the remaining reservoirs where polio is endemic are confined to india and contiguous countries and to sub-saharan africa. in 1999, the recommended polio eradication strategies (i.e., achieving and maintaining high routine vaccination coverage with oral poliovirus ... | 1999 | 10503575 |
| progress towards global poliomyelitis eradication. the approaching extinction of wild poliovirus type 2. | 1999 | 10511815 | |
| epidemiological analysis of immunity to poliovirus after termination of an era of vaccination with opv in germany. an analysis of the german association against viral diseases (dvv). | the global eradication of poliomyelitis by the year 2000 is an objective of the world health organization (who). since 1998, after a period of 37 years of vaccination using live oral poliovirus vaccine (opv), single use of inactivated poliovirus vaccine (ipv) has been recommended in germany. the present epidemiological analysis shows the immunity of 3474 patients to poliovirus types 1, 2 and 3 by using a microneutralization assay. a non-age-specific evaluation of antibodies seroprevalence to pol ... | 1999 | 10603664 |
| biology and pathological associations of the human papillomaviruses: a review. | historical cottontail rabbit papillomavirus studies raised early indications of a mammalian dna oncogenic virus. today, molecular cloning recognises numerous animal and human papillomaviruses (hpvs) and the development of in vitro transformation assays has escalated oncological research in hpvs. currently, their detection and typing in tissues is usually by southern blotting, in-situ hybridization and polymerase chain reaction methods. the complete papillomavirus virion constitutes a protein coa ... | 1998 | 10879257 |
| inactivation of human immunodeficiency virus type 1, hepatitis a virus, respiratory syncytial virus, vaccinia virus, herpes simplex virus type 1, and poliovirus type 2 by hydrogen peroxide gas plasma sterilization. | studies were conducted to determine the capability of a hydrogen peroxide gas plasma sterilization process to inactivate several types of viruses. six test agents were used: hiv type 1, human hepatitis a virus, respiratory syncytial virus, vaccinia, herpes simplex virus type 1, and poliovirus type 2. | 1998 | 9584802 |
| detection of human papillomavirus type 2 related sequence in oral papilloma. | oral papilloma is a benign tumourous lesion. part of this lesion is associated with human papillomavirus (hpv) infection. we analysed the genetical and histopathological evidence for hpv type 2 infection in three oral papillomas. southern blot hybridization showed hpv 2a sequence in one lesion. cells of the positive specimen appeared to contain high copy numbers of the viral dna in an episomal state. in situ staining demonstrated virus capsid antigen in koilocytotic cells and surrounding cells i ... | 1998 | 9699941 |
| a putative human papillomavirus type 57 new subtype isolated from plantar epidermoid cysts without intracytoplasmic inclusion bodies. | human papillomavirus type 60 (hpv-60) is the only virus type that has been identified in epidermoid cysts. in this study, hpv-57 dna was found in three out of 18 plantar epidermoid cysts with different histological features from hpv-60-associated cysts, using pcr and southern hybridization. the hpv-57-associated cysts had features resembling an hpv-2-specific cytopathic effect. the sequences of two hpv-57 dna clones isolated from two patients were identical, but differed at some positions from t ... | 1998 | 9714246 |
| presence of antibodies to human papillomavirus virus-like particles (vlps) in 11-13-year-old schoolgirls. | to allow meaningful approaches to vaccine development, it is important to know the extent of exposure to human papillomavirus (hpv) within the general population, and particularly the age at which the at risk population is infected. the humoral response to human papillomavirus is directed largely to conformationally-dependent epitopes on the whole virion. virus-like particles (vlps) of hpv types 1, 2, and 16 were produced using a baculovirus expression system, and were used in the intact state a ... | 1998 | 9783687 |
| identification of an alternative open reading frame ("hidden gene"?) stringently required for infectivity of poliovirus cdna clones. | translation of the uncapped poliovirus rna starts at the aug triplet spanning positions 741-743, and proceeds uninterrupted for almost the entire length of the genome. such a cap-independent mechanism of internal initiation of translation determines that a long, extra-cistronic region extends between the 5'-end and the main open reading frame (orf). we have identified 10 short orfs initiated by the alternative translation initiation codons acg, aua, and gug in the 5'-terminal extra-cistronic reg ... | 1998 | 9812311 |
| anti-picornavirus activity of synthetic flavon-3-yl esters. | the in vitro antiviral activity against picornaviruses (rhinovirus serotype 1b and 14, and poliovirus type 2) of new synthetic 3-hydroxyflavones, 3-acetoxyflavones, and substituted cinnamic and benzoic acid flavon-3-yl esters was evaluated. the maximum non-toxic concentration of compounds was determined in a human cell line (hela) suitable for the replication of the three viruses. their antiviral potency was measured by a plaque reduction assay. generally, rhinoviruses exhibited a higher sensiti ... | 1998 | 9865389 |
| enteroviruses in sewage: comparison of different technologies for wastewater treatment and reuse. | seven wastewater treatment plants were twice sampled to compare their different technologies for the removal of enteroviruses. these technologies are extended aeration, oxidation ditch, stabilization pond, aerated stabilization pond, submerged fixed film reactor, trickling filter and trickling filter followed by activated sludge in el-serw, el-rahamna, el-adliya, daqahla, el-barashiya, sohag and el-fayoum wastewater treatment plants, respectively. virus counts of raw sewage ranged between 2.7 x ... | 1997 | 17214145 |
| routine immunizations in adult renal transplant recipients. | vaccination guidelines for transplant recipients include regular boosters of tetanus, diphtheria, and inactivated polio vaccine, but there are few published data on the efficacy of these vaccines in patients receiving immunosuppressive therapy. | 1997 | 9089223 |
| a new who international reference reagent for use in potency assays of inactivated poliomyelitis vaccine. | assays of the potency of inactivated poliovirus vaccine require the use of an appropriate reference reagent. preparation 91/574 was shown by international collaborative study to be suitable for determination of antigenic content and immunogenicity of inactivated poliovirus vaccines by in vitro and in vivo assays, respectively. the reagent is a trivalent blend of formaldehyde-inactivated monovalent pools of poliovirus type 1 (mahoney) poliovirus type 2 (mef-1) and poliovirus type 3 (saukett). stu ... | 1997 | 9167009 |
| polio eradication in the world health organization south-east asia region by the year 2000: midway assessment of progress and future challenges. | in the south-east asia region (sear) of who, paralytic poliomyelitis has decreased from 25,711 cases in 1988 to 3304 cases in 1995, representing an 87% reduction. by 1995, in 6 of 10 member countries--india, bangladesh, myanmar, nepal, indonesia, and democratic people's republic of korea--polio remained endemic. two countries, sri lanka and thailand, appear close to polio eradication, and 2, bhutan and maldives, reported no cases during 1989-1995. although reported rates of acute flaccid paralys ... | 1997 | 9203699 |
| sequential use of inactivated poliovirus vaccine followed by oral poliovirus vaccine in oman. | seroprevalence and geometric mean titers (gmts) were compared at 6 and 10 months after vaccination with monovalent type 1 oral poliovirus vaccine (opv) at 6 months and trivalent opv at 7 and 9 months. group 1 had received 4 doses of opv, group 2 opv at birth and 3 doses of opv and inactivated poliovirus vaccine (ipv), and group 3 placebo at birth and 3 doses of ipv. a total of 547 infants completed the study. at 10 months, seroprevalence to poliovirus type 1 was 98%, 99%, and 98% in groups 1, 2, ... | 1997 | 9203722 |
| comparison of a monoclonal antibody-based igm capture elisa with a neutralization assay for assessing response to trivalent oral poliovirus vaccine. | monoclone-based igm capture elisas were developed for each of the three poliovirus serotypes and compared with a neutralization assay for detecting response to trivalent oral poliovirus vaccine among 224 infants. the igm-based response rates were significantly higher than the neutralizing antibody-based rates: 95% versus 83% to poliovirus type 1, 99% versus 94% to poliovirus type 2, and 89% versus 59% to poliovirus type 3. igm responses to the first vaccine dose were significantly associated bet ... | 1997 | 9203727 |
| clinical features and age distribution of patients with hpv 2/27/57-induced common warts. | the morphology of common warts depends on the inducing human papillomavirus (hpv) type. in order to assess the impact of the virus type on wart epidemiology we determined the virus type by pcr and recorded anamnestic data of 238 patients with common warts. warts induced by the related hpv types 2, 27 and 57 predominated in the study population (n = 202). these warts mostly occurred as multiple verrucae vulgares, mosaic warts or endophytic warts. patients aged between 10 and 30 years were most af ... | 1997 | 9209679 |
| structure of poliovirus type 2 lansing complexed with antiviral agent sch48973: comparison of the structural and biological properties of three poliovirus serotypes. | polioviruses are human pathogens and the causative agents of poliomyelitis. polioviruses are icosahedral single-stranded rna viruses, which belong to the picornavirus family, and occur as three distinct serotypes. all three serotypes of poliovirus can infect primates, but only type 2 can infect mice. the crystal structures of a type 1 and a type 3 poliovirus are already known. structural studies of poliovirus type 2 lansing (pv2l) were initiated to try to enhance our understanding of the differe ... | 1997 | 9261087 |
| common association of hpv 2 with anogenital warts in prepubertal children. | anogenital (ag) warts in 31 prepubertal children were hpv typed by nonisotopic in situ hybridization (nish) using digoxigenin-labeled probes for human papilloma virus (hpv) types 1-5, 6, 11, 16, 18, 31, and 33. mode of transmission was determined from historical, clinical, and laboratory data independent of hpv typing. hpv 2 was detected most commonly (13/31 warts) followed by hpv 6 (7/31), hpv 11 (5/31), and hpv 16 (1/31). although not reaching statistical significance, our results suggested th ... | 1997 | 9336800 |
| randomized comparison of early and late vaccination with inactivated poliovirus vaccine after allogeneic bmt. | forty-five adult hla-matched sibling bmt recipients were randomized to receive inactivated poliovirus vaccine (ipv) at 6, 8 and 14 months (early group, n = 23) or at 18, 20 and 26 months after bmt (late group, n = 22). ninety-five percent of the early group patients had protective antibody titres of > or = 4 to poliovirus type 1 (pv1), poliovirus type 2 (pv2) and poliovirus type 3 (pv3) by a microneutralization assay prior to the first vaccination, at 6 months after bmt. the corresponding propor ... | 1997 | 9383230 |
| [squamous intraepithelial lesions in young female university students]. | the authors investigated the frequency of squamous intraepithelial lesions (sil) in young women, in particular university students with special attention to morphological signs of the presence of human papilloma virus (hpv) in sil low grade-sil, lg-hpv. as compared with data in the world literature, they revealed a significantly lower rate of sil lg, hpv-2.81% as compared with 5.5% reported in who studies of young women. the authors analyzed the biological behaviour of sil, lg-hpv. as compared w ... | 1997 | 9424253 |
| phylogenetic analysis of the human papillomavirus type 2 (hpv-2), hpv-27, and hpv-57 group, which is associated with common warts. | human papillomavirus types 2 (hpv-2), hpv-27, and hpv-57, are three closely related viruses within the phylogenetic supergroup formed by the remotely related genital papillomaviruses. in contrast to this phylogenetic association, these three viruses are most often found in common warts at nongenital sites, but also occasionally in genital warts and mucosal lesions of the nasopharyngeal cavity. we studied the genomic diversity of hpv sequences in skin warts presumably caused by these viruses. the ... | 1997 | 9434721 |
| use of free-flow electrophoresis for the analysis of cellular uptake of picornaviruses. | free-flow electrophoresis is a powerful tool to separate subcellular vesicles such as early and late endosomes from plasma membranes. using this technique, the intracellular distribution of poliovirus type 2 sabin (pv2) and its derived subviral particles was analyzed upon infection of hela cells. comparison of various infection conditions showed that maximally 30% of total cell associated pv2 was found in endosomal compartments with the remainder being associated with plasma membrane fractions; ... | 1997 | 9527481 |
| effect of diarrhea on the humoral response to oral polio vaccination. | the purpose of this study was to measure the effect of concurrent diarrheal illness on seroconversion to trivalent oral polio vaccine (opv). | 1996 | 8852907 |
| [the sorption of poliovirus type 2 by a carbon sorbent]. | materials are presented concerning the study of a possibility to use highly active carbon sorbent "dnepr-mh" to liberate the tape water from enteroviruses. investigations were carried out on the model of the vaccine strain of type-ii poliovirus. the sorbent samples, most active in respect of the virus sorption and differing by benzene capacity, were determined. optimal concentration of a sorbent has been established, ph and water temperature were chosen under which the sorption processes are mor ... | 1996 | 9044704 |
| mouse neuropathogenic poliovirus strains cause damage in the central nervous system distinct from poliomyelitis. | poliomyelitis as a consequence of poliovirus infection is observed only in primates. despite a host range restricted to primates, experimental infection of rodents with certain genetically well defined poliovirus strains produces neurological disease. the outcome of infection of mice with mouse-adapted poliovirus strains has been described previously mainly in terms of paralysis and death, and it was generally assumed that these strains produce the same disease syndromes in normal mice and in mi ... | 1995 | 7476091 |
| effect of chloro-, cyano-, and amidino-substituted flavanoids on enterovirus infection in vitro. | synthetic flavans, isoflavans and isoflavenes substituted with chloro, cyano and amidino groups were tested for their in vitro activity against poliovirus type 2, coxsackie virus b4, echovirus type 6 and enterovirus 71. plaque-reduction assays showed that substituted 3-(2h)-isoflavenes, carrying a double bond in the oxygenated ring, possess antiviral activity higher than that of the corresponding isoflavans. the most effective compounds were 4'-chloro-6-cyanoflavan and 6-chloro-4'-cyanoflavan. s ... | 1995 | 7486950 |
| simultaneous administration of oral rhesus-human reassortant tetravalent (rrv-tv) rotavirus vaccine and oral poliovirus vaccine (opv) in thai infants. | rhesus-human reassortant tetravalent (rrv-tv) oral rotavirus vaccine was given at the same time as oral poliovirus vaccine (opv) or inactivated parenteral poliovirus vaccine (ipv) to thai infants at 2, 4 and 6 months of age. sera for rotavirus antibody studies were taken prior to and one month after each vaccination. after the first dose of vaccine at 2 months of age, 37% of the infants receiving rotavirus vaccine with ipv but only 10% of those receiving it with opv showed a seroconversion by ro ... | 1995 | 7625111 |
| prevalence of antibodies to polioviruses & enteroviruses excreted by healthy children in bombay. | a total of 132 healthy children between the ages one month and 12 yr were surveyed to determine the prevalence of antibodies to the three poliovirus serotypes. among infants up to six months of age, 73.2, 85.4 and 56.1 per cent had antibodies to poliovirus types 1, 2 and 3, respectively. in children of age groups 7 months to 3 yr and above 3 yr, antibody prevalence to the three poliovirus serotypes was 90.2, 86.9 and 57.4, and 83.3, 96.7 and 76.7 per cent, respectively. immunization coverage wit ... | 1995 | 7729847 |
| 5' utr of hepatitis a virus rna: mutations in the 5'-most pyrimidine-rich tract reduce its ability to direct internal initiation of translation. | the 5'-terminal untranslated region (5' utr) of the uncapped hepatitis a virus (hav) rna contains two pyrimidine-rich sequences; one about 20 nucleotides (nt) in length in the vicinity of the aug initiation codon (nt 706-726), and a longer one (about 40 nt) encompassing nt 100 to 140. the latter includes a 13 nt 'core' sequence (positions 126-138 in the hm175 strain) which is 80% identical to the pyrimidine-rich tract of poliovirus type 1 rna (mahoney strain). representative cdnas of the entire ... | 1995 | 7730803 |
| immune responses induced by administration of encapsidated poliovirus replicons which express hiv-1 gag and envelope proteins. | several viruses have been exploited for the development of recombinant vaccine vectors in which to express foreign proteins. recently, we have described a system utilizing the rna virus, poliovirus. we have constructed poliovirus genomes in which regions of the capsid have been substituted with gene fragments of the hiv gag and env genes. a complementation system has been designed to encapsidate defective genomes by providing the capsid protein in trans from a recombinant vaccinia virus (vv-p1). ... | 1995 | 8525684 |
| immunity to poliovirus and immunization with inactivated poliovirus vaccine after autologous bone marrow transplantation. | titers of antibody to poliovirus in 102 patients were determined with a sensitive neutralization assay before and 1 year after autologous bone marrow transplantation. at 1 year 14 patients (14%) had lost antibodies to poliovirus type 1 (p < .001), 10 (10%) to poliovirus type 2 (p < .05), and 13 (13%) to poliovirus type 3 (p < .01). twenty-two patients had lost antibodies to at least one type of poliovirus. follow-up of unimmunized patients 2 years (n = 40) and 3 years (n = 23) after transplantat ... | 1994 | 8038308 |
| [typification of a subgroup of cervical papilloma by in situ hybridization. preliminary study]. | the purpose of this study was to analyze 19 patients infected with human papilloma virus (hpv), in order to determine the most frequent subtype. subjects were patients of the national institute of perinatology (inper), between 1991-1992. all the patients had positive cytology, cervical biopsy was done through colposcopy control. sample characteristics were: mean age 29 years, married; most of them multiparous (9/19) and 7 were monogamous. six out of 12 cytologies were positive for hpv, 12 out of ... | 1994 | 8056363 |
| [riboprobes: an alternative in the detection of viral sequences]. | the use of riboprobes for the detection of rna viral sequences is analyzed. subgenomic fragments of cdna from poliovirus type 2, dengue virus type 4 and human immunodeficiency virus type 1, were inserted downstream sp6 and/or t7 promoters in the transcription vectors pgem-4z or psp64. rnas obtained by in vitro transcription in the presence of utp infinity (32p) were used as probes for the detection of rna viral sequences from infected cell lines in slot and northern blot assays. the poliovirus r ... | 1994 | 8073329 |
| cutaneous warts in butchers. | several studies have indicated a high prevalence of hand warts in meat handlers, although the reasons for this are not clear. the high prevalence may be partly due to hpv7, a virus found almost exclusively in meat handlers, but the source of hpv7 is not known. we have carried out a cross-sectional survey of hand warts in male meat workers and controls from other occupational groups, to investigate the reasons for the high prevalence of warts, and particularly of hpv7, in butchers. we studied 240 ... | 1994 | 8305325 |
| common warts from immunocompetent patients show the same distribution of human papillomavirus types as common warts from immunocompromised patients. | we studied the papillomaviruses (hpv) found in 131 common warts from 111 immunocompetent patients by amplification of viral dna sequences with the general-primer-mediated polymerase chain reaction (pcr). the virus types were determined by restriction-enzyme cleavage and reverse-blot analysis. results were confirmed by using the southern blot technique. forty patients harboured hpv 2a, 25 individuals showed hpv 2c and 13 yielded hpv 57. common warts from 16 patients were induced by a variant of h ... | 1993 | 8385983 |
| papillomavirus infection and its pathologic correlate in sexually active adolescents. | we investigated the prevalence of human papillomavirus (hpv) and its pathologic correlation in an adolescent clinic population (13-20 years, mean 16 years) over a 2-year period. 413 cervical specimens were obtained and analyzed cytologically and by a southern blot (sb) method for hpv dna. 277 specimens from 210 patients could be fully analyzed. 23 patients (10.9%) were positive for hpv dna by sb. cytologic findings in these 23 patients demonstrated changes compatible with low-grade squamous intr ... | 1993 | 8390646 |
| sch 38057: a picornavirus capsid-binding molecule with antiviral activity after the initial stage of viral uncoating. | the activity of a new water-soluble molecule, sch 38057, against picornaviruses is described. sch 38057 inhibited plaque formation of selected entero- and rhinoviruses in a range of 10.2 to 29.1 microm (50% endpoint) and had a therapeutic index of 10 against poliovirus type 2 (polio 2) in hela cells. when administered orally or subcutaneously, sch 38057 protected mice infected with either coxsackievirus b3 (cvb3) or echovirus-9 from mortality. the molecule provided a low level of protection agai ... | 1993 | 8391247 |
| childhood condyloma acuminatum: association with genital and cutaneous human papillomaviruses. | we studied 25 children, age 7 months to 12 years 6 months, with anogenital warts, and their parents. in most children the warts were localized in the anal area, in 3 of 18 girls perianally and on the vulva, and in 4 girls exclusively on the vulva. southern blot hybridization studies disclosed an association of condylomata with human papillomaviruses (hpv) 6 and 11 in 74% and hpv 2 in 17.4% of patients. the clinical features were similar in warts induced by genital and cutaneous hpvs. even the hp ... | 1993 | 8393994 |
| chiral discrimination and antipicornavirus activity of 6-oxazolinylisoflavan. | racemic 6-oxazolinylisoflavan, a highly effective inhibitor of rhinovirus serotype 1b in vitro, was resolved by high-performance liquid chromatography on a chiral stationary phase in order to study the activity of the enantiomers against picornaviruses. the absolute configuration of the two isomers was determined by circular dichroism curves. the antipicornavirus activity of each isomer, separately collected, was evaluated in vitro against human rhinovirus serotype 1b, enterovirus 71, echovirus ... | 1993 | 8398593 |
| [mouse neurovirulence of antigenic chimeras (type i/ii) of polioviruses]. | the mouse-adapted lansing strain of poliovirus type 2 pv-2(l) induces fatal poliomyelitis in mice after intracerebral inoculation, while mice inoculated with mahoney strain of poliovirus type 1 pv-1(m) show no signs of disease. previous work had indicated that both the 5' non-translated region of the viral genome and the viral capsid protein, neutralization antigenic site i (n-ag1), were involved in mouse neurovirulence. in order to further explore the role of n-ag1 in mouse neurovirulence, anti ... | 1993 | 7686824 |
| antigenic sequences of poliovirus recognized by t cells: serotype-specific epitopes on vp1 and vp3 and cross-reactive epitopes on vp4 defined by using cd4+ t-cell clones. | a panel of poliovirus-specific murine cd4+ t-cell clones has been established from both balb/c (h-2d) and cba (h-2k) mice immunized with sabin vaccine strains of poliovirus serotype 1, 2, or 3. t-cell clones were found to be either serotype specific or cross-reactive between two or all three serotypes. specificity analysis against purified poliovirus proteins demonstrated that t-cell clones recognized determinants on the surface capsid proteins vp1, vp2, and vp3 and the internal capsid protein v ... | 1992 | 1279196 |
| risk level, knowledge, and preventive behavior for human papillomaviruses among sexually active college women. | during the past decade, there has been mounting scientific evidence linking human papillomavirus (hpv) with cervical cancer and, at the same time, a great increase in physician consultations for hpv infections. hpv infection risk factors include multiple sex partners, early age at first intercourse, history of sexually transmitted diseases (stds), and smoking. this study surveyed 263 sexually active college women and concluded that (1) they are at considerable risk for contracting hpv, (2) they ... | 1992 | 1315349 |
| [condylomata acuminata in children--detection of hpv 6/11 and 2. local therapy with interferon-beta hydrogel]. | four cases of genital warts in children (girls) are reported. hpv 6/11-dna was identified in two cases, and hpv 2-dna in one. in one case no virus identification was possible. the clinical features of the hpv 2-induced genital warts showed the typical morphology of condylomata acuminata. the mode of transmission of the virus, in absence of sexual contact, could not be explained. the hpv 2-associated genital warts might have been transmitted by autoinoculation from warts on the hands. topical tre ... | 1992 | 1315724 |
| identification of candidate sequences that determine virulence in coxsackievirus b4. | we have previously shown that a major determinant of virulence for coxsackievirus b4 mapped to the 5' end of the viral genome. comparison of the corresponding cdna sequences of a virulent and a non-virulent virus has allowed the identification of candidate determinants of virulence in the 5' untranslated region and the capsid proteins vp1, vp2 and vp4. thirteen nucleotide substitutions were observed in a region spanning 3298 nucleotides. four mutations were detected in the non-coding region. of ... | 1992 | 1320798 |
| detection of enteroviruses in faeces by polymerase chain reaction. | a polymerase chain reaction (pcr) technique for the detection of human enteroviruses in stool specimens was developed. the test was based on the synthesis of cdna, followed by pcr and slot blot hybridization. the primers used were selected from a highly conserved sequence in the 5'non-coding region of the enteroviral genome. by this method 27 different enterovirus serotypes (15 echo, 6 coxsackie a, 4 coxsackie b, poliovirus type 2 and enterovirus 71) from 89 patients could be detected. using pos ... | 1992 | 1324520 |
| alterations of the p53 gene in human primary cervical carcinoma with and without human papillomavirus infection. | a previous report using cervical carcinoma cell lines suggests that the inactivation of two tumor suppressor gene products, p53 and prb, either by complex formation with the e6 and e7 proteins of oncogenic human papillomaviruses (hpvs) or by mutation, may be an important step in cervical carcinogenesis (m. scheffner et al., proc. natl. acad. sci. usa, 88: 5523-5527, 1991). the present study was designed to clarify the association between p53 inactivation and infection with oncogenic hpvs in prim ... | 1992 | 1327506 |
| inhibition of rotavirus in vitro transcription by optimal concentrations of monoclonal antibodies specific for rotavirus vp6. | three monoclonal antibodies (mabs) obtained from inoculation of mice with either a serotype 1 human rotavirus or rotavirus sa11 (serotype 3) inhibited the in vitro transcription of rotavirus sa11. two of the mabs exhibited a biphasic inhibitory response. removal of antibody from mab preparations by adsorption with sepharose-protein g reduced the inhibitory activity completely for all three mab preparations. analysis by radioimmunoprecipitation and western blotting indicated that all three mabs r ... | 1992 | 1331302 |
| purification and characterization of poliovirus polypeptide 3cd, a proteinase and a precursor for rna polymerase. | a cdna clone encoding the 3cd proteinase (3cdpro) of poliovirus type 2 (sabin), the precursor to proteinase 3cpro and rna polymerase 3dpol, was expressed in bacteria by using a t7 expression system. site-specific mutagenesis of the 3c/3d cleavage site was performed to generate active proteolytic precursors impaired in their ability to process themselves to 3cpro and 3dpol. of these mutations, the exchange of the thr residue at the p4 position of the 3c/3d cleavage site for a lys residue (3cdpro ... | 1992 | 1331532 |
| lymphoproliferative responses to human papillomaviruses in patients with cutaneous warts. | in vitro lymphoproliferative responses of peripheral blood mononuclear cells (pbmc) from patients with cutaneous warts, caused by infection with human papillomavirus type 1 (hpv-1) or type 2 (hpv-2), were assayed during the course of treatment. purified hpv-1 and hpv-2 were used as antigens, as well as herpes simplex virus (hsv) and concanavalin a (con a). all patients had normal percentages of subsets within the pbmc population and normal lymphoproliferative responses to con a, and those with a ... | 1992 | 1335750 |
| human papillomaviruses and skin cancer. | human papillomavirus (hpv)-induced skin warts are classically benign lesions. however an association between specific hpv types and skin cancer becomes obvious in epidermodysplasia verruciformis (ev). the analysis of this disease suggests that lesions infected with hpv types 5 and 8 carry a high risk of developing squamous cell carcinomas. the oncogenes of ev-viruses appear to be e6 and e2, rather than e7. the 'high risk' ev-viruses, hpv 5, 8, and 47, differ from related hpv types in the transfo ... | 1992 | 1335790 |
| epitope-mapped monoclonal antibodies against the hpv16e1--e4 protein. | the human papillomavirus (hpv) e1--e4 protein is the only nonstructural late protein encoded by the virus. we have isolated three hybridomas producing monoclonal antibodies to the e1--e4 protein of hpv16, which is the hpv type most frequently associated with cervical cancer. the three antibodies (tvg 401, 402, and 403) detect adjacent epitopes within the major seroreactive region of the molecule and show no reactivity against the e4 proteins of hpv1, hpv2, hpv4, or hpv6. the e1--e4 protein migra ... | 1992 | 1371027 |
| [relations of human papillomavirus and lesions of the oral mucosa]. | recently there has been an increased interest in the possible role that viruses and especially the human papilloma virus (hpv) could play in the etiology of lesions of the oral mucosa. a distinction has to be made between the so-called low-risk types of the virus (hpv-2, 6, 11, 13 and 32) which can be found in benign oral mucosal lesions, and the high-risk type (hpv-16), which predominantly is found in malignant oral mucosal lesions. | 1992 | 11894806 |
| a general primer pair for amplification and detection of genital human papillomavirus types. | a general primer pair localized in the e7 and e1 regions was identified and used for the detection of genital human papillomaviruses (hpvs). the genital hpv types 6b, 11, 16, 18, 31 and 33 were amplified and detected by the polymerase chain reaction (pcr) performed at a high stringency annealing temperature (60 degrees c). hpv-2, -3, -7, -13 and -30 were amplified only at lower temperatures. twelve biopsies from women with invasive cancer in the cervix were analysed with the general primer pair. ... | 1991 | 1650785 |
| structural and functional analysis of the ribosome landing pad of poliovirus type 2: in vivo translation studies. | the naturally uncapped genomic and mrnas of poliovirus initiate translation by an internal ribosome-binding mechanism. the mrna 5' untranslated region (utr) of poliovirus is approximately 750 nucleotides in length and has seven to eight (depending on the serotype) aug codons upstream of the initiator aug. the sequence required for internal ribosome binding has been termed the ribosome landing pad (rlp). to better understand the mechanisms of internal initiation, we have determined the boundaries ... | 1991 | 1656077 |
| in vitro mutational analysis of cis-acting rna translational elements within the poliovirus type 2 5' untranslated region. | initiation of translation on poliovirus rna occurs by internal binding of ribosomes to a region within the 5' untranslated region (utr) of the mrna. this region has been previously roughly mapped between nucleotides 140 and 631 of the 5' utr and termed the ribosome landing pad. to identify cis-acting elements in the 5' utr of poliovirus type 2 (lansing strain) rna that confer cap-independent internal initiation, we determined the in vitro translational efficiencies of a series of deletion and po ... | 1991 | 1656078 |
| human papillomavirus infection of the oral mucosa. | this article reviews the lesions of oral mucosa that contain human papillomavirus (hpv). these hpv-associated lesions can be classified into two broad types on the basis of their biologic behavior, benign lesions and premalignant malignant or malignant lesions. benign oral lesions include squamous cell papilloma (scp), verruca vulgaris (vv), condyloma acuminatum (ca), and focal epithelial hyperplasia (feh). of these entities, vv, ca, and feh demonstrate characteristic hpv-induced cytopathic effe ... | 1991 | 1656809 |
| analysis of neutralization-escape mutants selected from a mouse virulent type 1/type 2 chimeric poliovirus: identification of a type 1 poliovirus with antigenic site 1 deleted. | a chimeric type 1/type 2 poliovirus (v510), in which the antigenic site 1 (ag1) of poliovirus type 1 (pv-1) mahoney was replaced by the corresponding site of poliovirus type 2 (pv-2) lansing, is known to be neurovirulent for mice and neutralized by both type 1 and type 2 monoclonal antibodies. neutralization-escape mutants to monoclonal antibodies specifically recognizing the pv-2 sequence were obtained from v510. the nucleotide sequence and the mouse neurovirulence of mutants were determined. a ... | 1991 | 1707949 |
| the effect of site and mode of expression of a heterologous antigenic determinant on the properties of poliovirus hybrids. | the antigenic site normally situated in the ef loop of vp2 (2ef) of poliovirus type 2 (lansing) [pv2 (l)] was expressed in 2ef or in the bc loop of vp1 (1bc) of pv1 (mahoney) [pv1 (m)]. a hybrid virus expressing the site in 2ef of pv1 (m) is known to be neutralizable by pv2 (l)-specific antisera and to induce neutralizing antibodies against pv-2 (l). in contrast, a hybrid expressing a related sequence in 1bc of pv1 (m), which maintained the length of the native bc loop, was not neutralizable by ... | 1991 | 1713280 |
| identification of a consistent pattern of mutations in neurovirulent variants derived from the sabin vaccine strain of poliovirus type 2. | complete nucleotide sequencing of the rnas of two unrelated neurovirulent isolates of sabin-related poliovirus type 2 revealed that two nucleotides and one amino acid (amino acid 143 in the major capsid protein vp1) consistently departed from the sequences of the nonneurovirulent poliovirus type 2 712 and sabin vaccine strains. this pattern of mutation appeared to be a feature common to all neurovirulent variants of poliovirus type 2. | 1991 | 1850043 |
| notes on the pathogenesis of enterovirus infections. observations, experiments, and speculations. | despite extensive investigations over the last decades, several aspects of the pathogenesis of enterovirus infections in humans and animals are only poorly understood, such as the molecular basis of virulence (in particular of neurovirulence), tissue tropisms (e.g. the precise site of initial virus replication in the human intestinal tract), and precipitating factors of paralytic disease ("disposition"). studies on poliovirus myocarditis in man are reviewed. unequivocal evidence on replication o ... | 1990 | 1965548 |
| localization of genomic regions specific for the attenuated, mouse-adapted poliovirus type 2 strain w-2. | in order to begin to elucidate the genomic basis of the attenuation of mouse-adapted, poliovirus type 2 strain w-2 (pv2/w-2), we have cloned and sequenced the virus and compared it with the virulent, mouse-adapted pv2/lansing strain. in addition, we have performed computer-generated comparisons of pv2/w-2 to the non-mouse-adapted, attenuated pv2/sabin strain to determine whether mutational patterns occur that result in attenuation. the pv2/w-2 genome is 7434 nucleotides in length, which is three ... | 1990 | 2154539 |
| antibodies to polioviruses in an israeli population and overseas volunteers. | a seroepidemiological survey of prevalence of antibodies to poliovirus types 1-3 was carried out in 1,800 residents of 30 agricultural settlements (kibbutzim) in israel during 1980 and 1981. the survey showed that in 1980 84%, 90%, and 79% of the kibbutz residents exhibited antibodies to poliovirus types 1-3, respectively. in 1981 the figures were 81%, 91%, and 73%. significantly lower levels of antibodies for poliovirus type 3 in 1980/1981, and for poliovirus type 1 in 1981, were noticed in the ... | 1990 | 2154545 |
| presence and type specificity of papillomavirus antibodies demonstrable by immunoelectron microscopy tests in samples from patients with warts. | sera from 75 patients with warts were tested for antibodies against human papillomaviruses (hpvs) in an immunoelectron microscopy test. a group of 40 patients' sera were tested against a pooled suspension of the hpvs extracted from their warts and 90% of the sera were found to be antibody-positive. prior to serological investigation viruses from a second group of 35 patients were typed by dot blot hybridization with hpv-1 and hpv-2 dna. according to these results the patients were divided into t ... | 1990 | 2155296 |
| electronmicroscopic demonstration of hpv in oral warts. | human papilloma virus (hpv) has been demonstrated in a series of benign proliferative lesions of the skin and the mucosae. the virus has also been found in verrucous laryngeal carcinoma and carcinomas of the oral cavity and other organs. dna hybridization techniques have classified, the hpv into 51 types, some of which seem to be associated with specific lesions. in order to study the intracellular distribution of hpv, we performed ultrastructural analysis with the electron microscope on 14 spec ... | 1990 | 2155374 |
| activities and mechanisms of action of halogen-substituted flavanoids against poliovirus type 2 infection in vitro. | the effects of some halogen-substituted flavanoids (dichloroflavan, halogenated isoflavans, and isoflavenes) on poliovirus type 2 infection was examined. only two isoflavenes exhibited a significant inhibitory activity on the virus-induced cytopathic effect and plaque formation. in a single cycle of viral replication, both compounds reduced the viral yield by approximately 90%. the presence of the isoflavenes from the beginning of infection or during the adsorption period only prevented the shut ... | 1990 | 2159258 |
| human papillomavirus infection and filaggrin expression in paraffin-embedded biopsy specimens of extragenital bowen's disease and genital bowenoid papulosis. | cutaneous bowen's disease (bd) and genital bowenoid papulosis (bp) are considered as precancerous or cancerous lesions that are sometimes infected with human papillomavirus (hpv). we studied retrospectively paraffin-embedded sections of 11 samples of cutaneous bd and 6 samples of genital bp from the general population for hpv infection and filaggrin expression. using in situ hybridization with biotinylated probes of hpv types 1, 2, 5, 6, 11, 16, and 18, under stringent and/or non-stringent condi ... | 1990 | 2164515 |
| [eruptive viral warts of the burn area]. | eruptive viral warts were observed in a second-to-third degree burn area in a 68-year-old woman; the warts healed spontaneously and completely after approximately 3 weeks. hpv-2-related dna was demonstrated by southern blot analysis. | 1990 | 2166009 |
| a new antigenic site of poliovirus recognized by an intertypic cross-neutralizing monoclonal antibody. | a monoclonal antibody (mab 7j6) neutralizing poliovirus type 2 (pv2) and poliovirus type 1 (pv1) was obtained after immunization of balb/c mice with infectious pv2, strain mef-1. preincubation of mab 7j6 with pv1 inhibited its binding to pv2 and vice versa. neutralization-resistant variants of pv2 and pv1 were selected. nucleotide sequencing of the rnas of some variants revealed mutations in the loop of amino acid residues 239 to 245 in vp2 and in the loop of amino acid residues 195 to 207 in vp ... | 1990 | 2171198 |
| severe intractable common warts associated with human papillomavirus 2, 3 and 20. | a 26-year-old male had had numerous common warts on the feet and hands since childhood. they had repeatedly undergone etretinate cryosurgery, always responding well to it. however, the warts reappeared. human papillomavirus (hpv) 2, 3 and 20 were detected in the warts using southern's blot hybridization technique with hpv dna. histologically, the warts showed swollen, clear keratinocytes with vacuolated degeneration not found in common warts. the findings are presented in this paper. | 1990 | 2173668 |
| anti-thymocyte serum delays clearance of poliovirus from the mouse central nervous system. | antibody is of primary importance for protection from poliovirus-induced paralysis (poliomyelitis) and from other enterovirus infections. however, the components of the immune response involved in the clearance of an established enterovirus infection of the central nervous system (cns) are not known. to assess the effect of thymus-dependent immune functions on a cns poliovirus infection, adult balb/c mice inoculated intracerebrally with the w-2 strain of human poliovirus type 2 (pv2) were treate ... | 1989 | 2539386 |
| mapping of attenuating sequences of an avirulent poliovirus type 2 strain. | a mouse model for poliomyelitis was used to identify genomic sequences that attenuate neurovirulence of poliovirus strain p2/p712. this type 2 strain is avirulent in primates and mice yet grows as well as virulent strains in cell culture. the approach used was to exchange portions of the genome of the mouse-virulent p2/lansing strain with the corresponding region from p2/p712 to identify sequences that could attenuate lansing neurovirulence in mice. a full-length infectious cdna of p2/p712 was a ... | 1989 | 2539491 |
| clearance of a persistent human enterovirus infection of the mouse central nervous system by the antiviral agent disoxaril. | enteroviruses can cause persistent central nervous system (cns) infections in agammaglobulinemic individuals. because these infections are rarely cured by passive administration of antibody, a chemotherapeutic approach would be advantageous. in this study, the efficacy of the antienterovirus (and antipicornavirus) drug disoxaril was demonstrated in a murine model of persistent enterovirus infection. disoxaril is a hydrophobic antiviral compound that blocks picornavirus uncoating. the w-2 strain ... | 1989 | 2540248 |
| anogenital warts in childhood. | fifteen children with anogenital warts are presented. twelve cases were referred for assessment of sexual abuse which was established in six cases, strongly suspected in one, and excluded in three. in two, the source was unclear. papillomavirus typing was carried out by hpv dna dot and southern blot hybridization using mixed hpv 6/11, 16/18, and 2/3 dna probes on 15 specimens from 12 of the children. seven biopsy specimens were positive for hpv 6 or 11 and one hybridized with both hpv 6/11 and 1 ... | 1989 | 2545318 |
| [ultrastructural demonstration of the human papilloma virus (hpv) in oral proliferative lesions]. | the human papilloma virus (hpv) has been found in a series of benign proliferative lesions of the skin and mucosa. the virus has also been found in verrucous laryngeal carcinoma and carcinomas of the oral cavity and other organs. dna hybridization techniques have made it possible to classify 51 types of hpvs, some appearing to be associated with specific lesions. in order to study the intracellular distribution of hpvs, an ultrastructural morphological analysis was performed with an electron mic ... | 1989 | 2548368 |
| sequence divergence yet conserved physical characteristics among the e4 proteins of cutaneous human papillomaviruses. | human papillomavirus (hpv) types 1, 2, and 4 together comprise the major cause of cutaneous papillomas in the general population. we have aligned the genomes of these three viruses by partial sequence analysis, and have sequenced the e4 open reading frames (orfs) of hpv 2 and hpv 4. after expression as beta-gal fusion proteins in bacteria, antibodies raised to the putative e4 gene-products of both virus types were used to identify the native e4 proteins in naturally occurring tumors. at the prim ... | 1989 | 2549722 |
| molecular pathogenesis of type 2 poliovirus in mice. | lansing strain of poliovirus type 2 (pv-2) produces a fatal paralytic disease in mice after intracerebral inoculation. to identify virus-containing nerve cells in poliovirus-infected mice, we developed a technique of in situ hybridization for viral rna with a poliovirus-specific riboprobe. large numbers of genomes were found in motoneurons and their processes, as well as in ghosts of neurons with or without inflammatory cells. this indicates that the death of motoneurons is due to a direct effec ... | 1989 | 2551721 |
| human papillomavirus type 29 (hpv-29), an hpv type cross-hybridizing with hpv-2 and with hpv-3-related types. | the cloning and partial characterization of human papillomavirus (hpv) type 29 is presented. by hybridization analyses, this virus appears to be related to hpv types associated with common warts and hpv types associated with flat warts. | 1989 | 2552165 |
| detection of herpes simplex virus dna in a cutaneous squamous cell carcinoma by in situ hybridization. | a case of a cutaneous squamous cell carcinoma (scc) occurring at the site of a long-standing recurrent hsv infection is described. no deficit of the cell-mediated immunity was recorded. hsv2 was isolated in several viral cultures. hsv dna was visualized in the scc by in situ hybridization with biotinylated probes in paraffin-embedded tissue. the samples were negative for hpv2, 5, 16, and 18 probes. a causal relationship between hsv infection and cutaneous scc is hypothesized. | 1989 | 2552936 |
| inhibition of translation in cells infected with a poliovirus 2apro mutant correlates with phosphorylation of the alpha subunit of eucaryotic initiation factor 2. | a poliovirus type 2 lansing mutant was constructed by inserting 6 base pairs into the 2apro region of an infectious cdna clone, resulting in the addition of a leucine and threonine into the polypeptide sequence. the resulting small-plaque mutant, 2a-2, had a reduced viral yield in hela cells and synthesized viral proteins inefficiently. infection with the mutant did not lead to specific inhibition of host cell protein synthesis early in infection, and this defect was attributed to a failure to i ... | 1989 | 2555543 |
| novel variants of human papillomavirus type 2 in warts from immunocompromised individuals. | twenty cutaneous warts from i8 renal transplant recipients (rtr) and a single wart sample from a patient suffering from hodgkin's disease were screened for the presence of human papillomavirus (hpv) sequences by southern blot analysis. thirteen samples were shown to contain hpv dna sequences, one was identified as hpv-3, two as hpv-4, six as hpv-2 and four were unidentifiable by restriction digest analysis. hpv-3 was identified in the wart sample from the patient suffering from hodgkin's disease ... | 1989 | 2557072 |
| [isolation of recombinant vaccine strains of poliovirus from patients with poliomyelitis]. | oligonucleotide maps of some poliovirus type 2 strains isolated from polio cases, while being clearly related to that of the sabin vaccine type 2 strain, exhibited, nevertheless, marked differences from the reference (vaccine) map. several large oligonucleotides derived from 4 such strains were subjected to enzymatic sequencing. the results strongly suggest that all of them were intertypic recombinants between the sabin strains. the 5'-parts of the genomes of these strains were derived from the ... | 1989 | 2560812 |
| a human-human hybridoma which produces antibodies to polioviruses. | a human-human hybridoma which produced antibodies to sabin strain poliovirus types 3 and 2 was established. this hybridoma was constructed by fusing a hat-sensitive variant of a human lymphoblastoid subline r4-4-6 with peripheral blood lymphocytes of a donor who had been previously immunized with poliovirus sabin vaccines and had shown high titer of neutralizing antibodies against these viruses. this hybridoma had been cultured for more than one year and the neutralizing activities of the cultur ... | 1989 | 2561573 |
| warts in fish handlers. | fish handlers frequently suffer from hand warts. the clinical form and hpv type in these lesions were studied. eleven individuals (10 fishmongers and one fisherman) with multiple hand warts were examined clinically and samples from their warts examined by southern blot and reverse blot analysis. clinically, with one exception, the warts were of the common type. hpv dna was detected in all but one individual. hpv4 was found in one sample, hpv1 related virus in three, a virus hybridizing with both ... | 1989 | 2540791 |
| humoral and cellular immunity to papillomavirus in patients with cervical dysplasia. | the cell-mediated and humoral immune responses to human papillomavirus (hpv) were tested in groups of patients with various degrees of cervical intraepithelial neoplasia (cin) using a lymphocyte proliferation assay (lpa) as a measure of circulating sensitised t-cells and an enzyme-linked immunosorbent assay (elisa) for antibodies. twenty-three of 92 patients (25%) gave stimulation indices (s.i.) greater than two to at least one of the several antigen preparations tested in the lpa. of 282 patien ... | 1988 | 2828535 |
| chronic enteric virus infection in two t-cell immunodeficient children. | enteric virus infections were studied in two children with congenital t-cell immunodeficiency. one patient (lc) with cartilage hair hypoplasia developed persistent diarrhea and malabsorption following acute gastroenteritis. electron microscope (em) examination of feces revealed excretion of rotavirus for more than 450 days with concurrent astrovirus infection for at least 225 days, associated with the persistent diarrhea. prolonged infection with poliovirus type 2 following vaccination had previ ... | 1988 | 2835434 |
| oral squamous papillomas: detection of hpv dna by in situ hybridization. | oral squamous papillomas were segregated from other papillary lesions on the basis of histopathologic features. twenty representative papillomas were evaluated for the presence of papillomavirus genus-specific antigen with the use of an immunoperoxidase technique. these same tumors were analyzed for human papillomavirus (hpv) types 2, 4, 6, and 11 with biotinylated full-length double-stranded dna probes by in situ hybridization. only one case exhibited papillomavirus antigen reactivity. alternat ... | 1988 | 2836773 |
| poliovirus host range is determined by a short amino acid sequence in neutralization antigenic site i. | the mouse-adapted strain of poliovirus type 2 (lansing) induces fatal poliomyelitis in mice after intracerebral inoculation, whereas mice inoculated with poliovirus type 1 (mahoney) show no signs of disease. previous work indicated that the adaptation to mouse virulence is associated with the viral capsid proteins and that mutations in neutralization antigenic site i of poliovirus reduce neurovirulence of the lansing strain in mice. the role of antigenic site i in mouse neurovirulence was furthe ... | 1988 | 2838906 |
| epidemiology of butchers' warts. | studies were carried out in two slaughter-houses in different cities differing in the degree of work automation and, for comparison, in workers of nearby factories of the same two cities. there was a high incidence of warts (49.2%) in a slaughterhouse where the workers had direct contact with animals and meat, while a significantly lower incidence (9%) was observed in a modern slaughterhouse where the work was almost completely automated. the types of human papillomaviruses (hpvs) were similar i ... | 1988 | 2841908 |
| coxsackievirus b-3 selection of virus resistant buffalo green monkey kidney cells and chromosome analysis of parental and resistant cells. | following coxsackievirus b-3 (cbv-3) infection and lysis of highly susceptible buffalo green monkey kidney (bgmk) cells, there was a regrowth of cells. cultures of regrown cells were established and they continued to release infectious cbv-3 for up to 20 weeks. the parental bgmk cells were susceptible to cbv-3, cbv-4 and poliovirus type 2 induced cytopathic effect (cpe), while the cured cells were resistant to cbv-3 and cbv-4 but not to poliovirus type 2. attachment of cbv-3 was restricted on cu ... | 1988 | 2845893 |
| mutational analysis of upstream aug codons of poliovirus rna. | the 5' untranslated region of poliovirus type 2 lansing rna consists of 744 nucleotides containing seven aug codons which are followed by in-frame termination codons, thus forming short open reading frames (orfs). to determine the biological significance of these small orfs, all of the upstream aug codons were mutated to uug. the point mutations were introduced into an infectious poliovirus cdna clone, and rna transcribed in vitro from the altered cdna was transfected into hela cells to recover ... | 1988 | 2846865 |
| poliovirus proteinase 3c: large-scale expression, purification, and specific cleavage activity on natural and synthetic substrates in vitro. | proteinase 3c of poliovirus type 2 (sabin) was expressed at 4% total protein in escherichia coli. the protein was soluble and could be purified by a simple scheme. it was weakly active on the capsid precursor p1 (expressed in vitro), which contains two cleavage sites. the products of processing p1 were 1abc and 1d (vp1). the activity was insensitive to triton x-100. crude extracts of cells infected with poliovirus type 1 (mahoney) gave strong processing and yielded 1ab (vp0), 1c (vp3), and 1d in ... | 1988 | 2846872 |
| antibodies to poliomyelitis virus in cord blood of neonates born to from mothers in contact or not in contact with the wild virus. | neutralizing antibodies to polioviruses in cord blood of neonates born from 64 mothers under age 20 and in 53 mothers aged 30 years and over were investigated in order to know and compare the transfer to newborns of antibodies to polioviruses produced by live oral vaccine mainly and those antibodies induced by natural contact with wild poliovirus strains. total immunity for the two groups was higher than 80% for the three types of polioviruses, with only virus 3 showing an immunity below 80% (77 ... | 1988 | 2848883 |
| detection of ebv and hpv dna sequences in oral "hairy" leukoplakia by in situ hybridization. | oral "hairy" leukoplakia (ohl) is a white lesion of the oral mucosa, usually located on the lateral tongue among human immunodeficiency virus (hiv) positive acquired immunodeficiency syndrome (aids)-risk patients. the lesion has been reported to be associated with epstein-barr virus (ebv) and human papillomavirus (hpv). twenty surgical biopsy specimens were evaluated for the presence of hpv genus-specific antigen, hpv 2/4, 6/11, and 16/18 dna and ebv dna by in situ hybridization employing formal ... | 1988 | 2849634 |