Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| inactivation of hantaviruses by n-ethylmaleimide preserves virion integrity. | thiol groups of cysteine residues are crucial for the infectivity of various enveloped viruses, but their role in the infectivity of viruses of the family bunyaviridae has thus far not been studied. this report shows that thiol groups are essential to the infectivity of hantaviruses. alkylation of the thiol functional groups using the membrane-permeable compound n-ethylmaleimide (nem) and membrane-impermeable compound 5,5'-dithio-bis-(2-nitrobenzoic acid) (dtnb) showed nem to be a highly effecti ... | 2011 | 21289161 |
| genetic diversity of imjin virus in the ussuri white-toothed shrew (crocidura lasiura) in the republic of korea, 2004-2010. | recently, imjin virus (mjnv), a genetically distinct hantavirus, was isolated from lung tissues of the ussuri white-toothed shrew (crocidura lasiura) captured near the demilitarized zone in the republic of korea. to clarify the genetic diversity of mjnv, partial m- and l-segment sequences were amplified from lung tissues of 12 of 37 (32.4%) anti-mjnv igg antibody-positive ussuri white-toothed shrews captured between 2004 and 2010. a 531-nucleotide region of the m segment (coordinates 2,255 to 2, ... | 2011 | 21303516 |
| interview with pedro fernando da costa vasconcelos, md, phd. interview by vicki glaser. | 2011 | 21261512 | |
| hantavirus infection during a stay in a mountain hut in northern slovakia. | hantaviruses in europe cause human hemorrhagic fever with renal syndrome (hfrs) with various degree of severity. the most severe form is caused by the dobrava/belgrade virus (dobv), associated with the rodent apodemus flavicollis. during the last decade cases of infection caused by dobv have been reported in central europe. the present study is a report on two czech patients with severe hfrs who were infected during their stay in a mountain hut in northern slovakia. the two patients, combined wi ... | 2011 | 21264871 |
| delayed density-dependent prevalence of sin nombre virus infection in deer mice (peromyscus maniculatus) in central and western montana. | understanding how transmission of zoonoses takes place within reservoir populations, such as sin nombre virus (snv) among deer mice (peromyscus maniculatus), is important in determining the risk of exposure to other hosts, including humans. in this study, we examined the relationship between deer mouse populations and the prevalence of antibodies to snv, a system where the effect of host population abundance on transmission is debated. we examined the relationship between abundance of deer mice ... | 2011 | 21269997 |
| genetic evidence for the presence of two distinct hantaviruses associated with apodemus mice in croatia and analysis of local strains. | in europe, dobrava-belgrade (dobv), saaremaa (saav), and puumala (puuv) viruses are known to cause hemorrhagic fever with renal syndrome (hfrs). all three hantaviruses are now found in croatia. lung tissue samples of 315 apodemus mice trapped in 2003-2004 were screened for the presence of hantaviral n-ag and 20 mice (6.3%) were found either strongly positive or weak/suspected-positive. partial sequences of hantavirus m and s segments were recovered by rt-pcr from six mice and subjected to (phylo ... | 2011 | 21108346 |
| procalcitonin in hantavirus infections. | in hantavirus infections levels of serum leukocytes or c-reactive protein are usually elevated to levels found in serious bacterial infections. however, procalcitonin in patients infected with hantavirus has not yet been discussed in the literature. a total of 29 adult patients with hantavirus infection, 30 with sepsis, and 19 with tick-borne encephalitis were included in this observational retrospective study. the median procalcitonin level in patients with hantavirus infection was 0.53 µg/l (r ... | 2011 | 21344982 |
| detection of crimean-congo hemorrhagic fever, hanta, and sandfly fever viruses by real-time rt-pcr. | the development of sensitive and specific nucleic acid diagnostic assays for viral pathogens is essential for proper medical intervention. this chapter describes four fluorescence-based pcr assays to detect the crimean-congo hemorrhagic fever (cchfv), andes (andv), hantaan (hanv), and sandfly fever sicilian (sfsv) viruses. these assays are based on species-specific hydrolysis probes targeting the nucleocapsid protein gene for cchfv and sfsv and the glycoprotein gene for andv and hanv. all four a ... | 2011 | 21116810 |
| arenaviruses and hantaviruses: from epidemiology and genomics to antivirals. | the arenaviruses and hantaviruses are segmented genome rna viruses that are hosted by rodents. due to their association with rodents, they are globally widespread and can infect humans via direct or indirect routes of transmission, causing considerable human morbidity and mortality. nevertheless, despite their obvious and emerging importance as pathogens, there are currently no effective antiviral drugs (except ribavirin which proved effective against lassa virus) with which to treat humans infe ... | 2011 | 21356244 |
| hantavirus-specific iga in saliva and viral antigen in the parotid gland in patients with hemorrhagic fever with renal syndrome. | the hantavirus genus comprises rodent borne, zoonotic viruses of the bunyaviridae family that cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus cardiopulmonary syndrome (hcps) in the americas. rodent saliva contains infectious hantavirus and evidence suggests that hantavirus is also shed in human saliva, but person-to-person transmission is rare. in saliva, immunoglobulin (ig) a is the predominant immunoglobulin class. secretory iga serves as an important first line of ... | 2011 | 21360546 |
| using geographic information system-based ecologic niche models to forecast the risk of hantavirus infection in shandong province, china. | hemorrhagic fever with renal syndrome (hfrs) is an important public health problem in shandong province, china. in this study, we combined ecologic niche modeling with geographic information systems (gis) and remote sensing techniques to identify the risk factors and affected areas of hantavirus infections in rodent hosts. land cover and elevation were found to be closely associated with the presence of hantavirus-infected rodent hosts. the averaged area under the receiver operating characterist ... | 2011 | 21363991 |
| genetic evidence of hantavirus infections in wild rodents from northwestern colombia. | this report builds on recent serological evidence for the presence of hantavirus in northern colombia by providing sequence-specific and phylogenetic data of hantavirus infections in wild rodents. from august 2007 to august 2008, 354 rodent specimens representing four families were collected in the northwestern antioquia region of colombia. antibodies reactive to sin nombre virus and maciel virus antigens by igg enzyme-linked immunosorbent assay were found in 15 of 109 (14%) cherries cane rats ( ... | 2011 | 21284514 |
| characterization of the interaction between hantavirus nucleocapsid protein (n) and ribosomal protein s19 (rps19). | hantaviruses, members of the bunyaviridae family, are negative-stranded emerging rna viruses and category a pathogens that cause serious illness when transmitted to humans through aerosolized excreta of infected rodent hosts. hantaviruses have evolved a novel translation initiation mechanism, operated by nucleocapsid protein (n), which preferentially facilitates the translation of viral mrnas. n binds to the ribosomal protein s19 (rps19), a structural component of the 40 s ribosomal subunit. in ... | 2011 | 21296889 |
| sero-epidemiological study of the presence of hantaviruses in domestic dogs and cats from belgium. | hantaviruses are worldwide rodent-borne pathogens infecting humans and other animals mainly through inhalation of aerosols contaminated with rodent excreta. few data are available on hantavirus serology and geographical distribution in dogs and cats. we therefore screened sera from pet dogs (n=410) and cats (n=124) in two regions of belgium, using igg elisa and ifa. we analysed the effect of the owner's address as well as pet gender and age on hantavirus status. hantavirus antibodies were found ... | 2011 | 21388647 |
| seroepidemiological survey of hantavirus infection in healthy people in vallès occidental, barcelona. | hantaviruses are the etiological agents of hemorrhagic fever with renal syndrome in europe and asia, and hantavirus pulmonary syndrome in america. approximately 150,000 cases are reported annually worldwide. in spain, some hantavirus infection cases have been described. besides, rodents that have been described as hantavirus reservoirs are present. the aim of the present study was to determinate the seroprevalence of hantavirus in humans in the northeast of spain. | 2011 | 21417923 |
| comparison of hantaan and seoul viral infections among patients with hemorrhagic fever with renal syndrome (hfrs) in heilongjiang, china. | hemorrhagic fever with renal syndrome (hfrs) is a serious public health problem in china, and is primarily caused by either the hantaan virus (htnv) or seoul virus (seov) strains. however, the causative hantavirus has only been definitively identified in a few hfrs cases, and detailed comparisons of patient data for the 2 strains are limited. | 2011 | 21428852 |
| concomitant influence of helminth infection and landscape on the distribution of puumala hantavirus in its reservoir, myodes glareolus. | puumala virus, the agent of nephropathia epidemica (ne), is the most prevalent hantavirus in europe. the risk for human infection seems to be strongly correlated with the prevalence of puumala virus (puuv) in populations of its reservoir host species, the bank vole myodes glareolus. in humans, the infection risks of major viral diseases are affected by the presence of helminth infections. we therefore proposed to analyse the influence of both helminth community and landscape on the prevalence of ... | 2011 | 21303497 |
| serosurvey of hantavirus infection in humans in the border region between brazil and argentina. | according to reports by the ministry of health, in the far western region of the state of santa catarina, there have been no reports of hantavirus pulmonary syndrome, a zoonotic disease transmitted by feces of infected rodents. a seroepidemiological study of residents of this region, was conducted, with the aim of determining the presence of hantavirus infections. a total of 340 volunteers of both genus, from the towns of belmonte and paraíso, were studied. | 2011 | 21468477 |
| viral infections in workers in hospital and research laboratory settings: a comparative review of infection modes and respective biosafety aspects. | to compare modes and sources of infection and clinical and biosafety aspects of accidental viral infections in hospital workers and research laboratory staff reported in scientific articles. | 2011 | 21497126 |
| structural characterization of the crimean-congo hemorrhagic fever virus gn tail provides insight into virus assembly. | the rna virus that causes the crimean congo hemorrhagic fever (cchf) is a tick-borne pathogen of the nairovirus genus, family bunyaviridae. unlike many zoonotic viruses that are only passed between animals and humans, the cchf virus can also be transmitted from human to human with an overall mortality rate approaching 30%. currently, there are no atomic structures for any cchf virus proteins nor for any nairovirus proteins. a critical component of the virus is the envelope gn glycoprotein, which ... | 2011 | 21507948 |
| human pathogenic hantaviruses and prevention of infection. | hantaviruses are emerging viruses which are hosted by small mammals. when transmitted to humans, they can cause two clinical syndromes, hemorrhagic fever with renal syndrome or hantavirus cardiopulmonary syndrome. the review compiles the current list of hantaviruses which are thought to be pathogenic in humans on the basis of molecular or at least serological evidence. whereas induction of a neutralizing humoral immune response is considered to be protective against infection, the dual role of c ... | 2011 | 21508676 |
| maporal virus as a surrogate for pathogenic new world hantaviruses and its inhibition by favipiravir. | pathogenic hantaviruses geographically distributed in the old world cause haemorrhagic fever with renal syndrome (hfrs), whereas new world hantaviruses are the aetiological agents of hantavirus cardiopulmonary syndrome (hcps). ribavirin, a drug associated with toxicities, is presently indicated for treatment of hfrs, whereas treatment of the more frequently lethal hcps is limited to supportive care. because of the need for safe and effective antivirals to treat severe hantaviral infections, we e ... | 2011 | 21566265 |
| infection of human monocyte-derived dendritic cells by andes hantavirus enhances pro-inflammatory state, the secretion of active mmp-9 and indirectly enhances endothelial permeability. | abstract: | 2011 | 21569520 |
| [investigation on the natural infectious status of hantaviruses among small mammals in longquan city, zhejiang province]. | to investigate the situation of the natural infection of hantaviruses (hv) in small mammals and to provide evidence for the control and prevention of hemorrhagic fever with renal syndrome (hfrs) in longquan area, zhejiang province. | 2011 | 21781480 |
| presence of activated airway t lymphocytes in human puumala hantavirus disease. | hantaviruses cause two clinical syndromes: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). the clinical spectrum in hfrs also often involves respiratory symptoms. as information about the pulmonary pathogenesis in hfrs is limited, we aimed to further study the local airway immune response in the lower airways. | 2011 | 21436245 |
| prevalence of hantavirus infection in wild rodents from five provinces in korea, 2007. | hemorrhagic fever with renal syndrome (hfrs) is an infectious disease caused by hantaviruses of the family bunyaviridae. among them, hantaan virus (htnv) is most widely distributed in korea. the striped field mouse, apodemus agrarius, is the natural host of htnv in rural korea. we trapped 766 small mammals of three species (1 eothenomys regulus, 13 crocidura suaveolens, and 752 apodemus agrarius) in five provinces in korea from january to december 2007. we tested 542 rodent sera for htnv antibod ... | 2011 | 21441196 |
| detection of hantaviruses in brazilian rodents by sybr-green-based real-time rt-pcr. | current knowledge of the pathogenic hantavirus indicates that wild rodents are its primary natural reservoir. specific primers to detect the presence of viral genomes were developed using an sybr-green-based real-time rt-pcr protocol. one hundred sixty-four rodents native to the atlantic forest biome were captured in são paulo state, brazil, and their tissues were tested. the presence of hantavirus rna was detected in sixteen rodents: three specimens of akodon montensis, three of akodon cursor, ... | 2011 | 21442233 |
| hantavirus pulmonary syndrome, united states, 1993-2009. | hantavirus pulmonary syndrome (hps) is a severe respiratory illness identified in 1993. since its identification, the centers for disease control and prevention has obtained standardized information about and maintained a registry of all laboratory-confirmed hps cases in the united states. during 1993-2009, a total of 510 hps cases were identified. case counts have varied from 11 to 48 per year (case-fatality rate 35%). however, there were no trends suggesting increasing or decreasing case count ... | 2011 | 21762572 |
| recent advances in hantavirus molecular biology and disease. | hantaviruses are emerging zoonotic pathogens that belong to the bunyaviridae family. they have been classified as category a pathogens by cdc (centers for disease control and prevention). hantaviruses pose a serious threat to human health because their infection causes two highly fatal diseases, hemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps). these pathogens are transmitted to humans through aerosolized excreta of their infected rodent hosts. hantavir ... | 2011 | 21459193 |
| epidemiological study on hemorrhagic fever with renal syndrome in flood areas. | to investigate the infection of hemorrhagic fever with renal syndrome (hfrs) in host animals and inapparent infection of hfrs in general population in dongting lake areas after floods. | 2011 | 21464543 |
| complement activation in puumala hantavirus infection correlates with disease severity. | abstract introduction. hantaviruses are important human pathogens that cause clinical diseases characterized by renal and cardiopulmonary manifestations. their pathogenesis is currently poorly understood. we have studied the role of the complement system in the pathogenesis of puumala (puuv) hantavirus infection. material and methods. we studied the activation of complement by measuring the terminal complement complex sc5b-9 and complement component c3 and c4 levels in patients with acute puuv i ... | 2011 | 21495786 |
| biogeographic and ecological regulation of disease: prevalence of sin nombre virus in island mice is related to island area, precipitation, and predator richness. | the relative roles of top-down and bottom-up forces in affecting disease prevalence in wild hosts is important for understanding disease dynamics and human disease risk. we found that the prevalence of sin nombre virus (snv), the agent of a severe disease in humans (hantavirus pulmonary syndrome), in island deer mice from the eight california channel islands was greater with increased precipitation (a measure of productivity), greater island area, and fewer species of rodent predators. in findin ... | 2011 | 21508614 |
| platelet ligands and adamts13 during puumala hantavirus infection and associated thrombocytopenia. | we aimed here to elucidate the role of adhesive platelet ligands and endothelial involvement during the acute phase of puumala hantavirus (puuv) infection. nineteen hospital-treated patients with serologically confirmed diagnosis of acute puuv infection were included. patient charts were reviewed for clinical and basic laboratory data. plasma levels of von willebrand factor antigen (vwf: ag), ristocetin cofactor (vwf: rco), factor viii (fviii: c) and a disintegrin and metalloproteinase with a th ... | 2011 | 21508829 |
| the delicate pygmy rice rat (oligoryzomys delicatus) is the principal host of maporal virus (family bunyaviridae, genus hantavirus). | abstract choclo virus (chov) and maporal virus (mapv) are enzootic in panama and western venezuela, respectively. the results of previous studies suggested that the fulvous pygmy rice rat (oligoryzomys fulvescens) is the principal host of both viruses. the results of an analysis of nucleotide sequence data in this study indicated that the rodent associated with chov is the costa rican pygmy rice rat (oligoryzomys costaricensis) and that the rodent associated with mapv is the delicate pygmy rice ... | 2011 | 21548760 |
| expression of recombinant araraquara hantavirus nucleoprotein in insect cells and its use as an antigen for immunodetection compared to the same antigen expressed in escherichia coli. | abstract: background: antigens for hantavirus serological tests have been produced using dna recombinant technology for more than twenty years. several different strategies have been used for that purpose. all of them avoid the risks and difficulties involved in multiplying hantavirus in the laboratory. in brazil, the araraquara virus is one of the main causes of hantavirus cardio-pulmonary syndrome (hcps). methods. in this investigation, we report the expression of the n protein of the araraqua ... | 2011 | 21569341 |
| notes from the field: hantavirus pulmonary syndrome --- maine, april 2011. | on april 25, 2011, the maine center for disease control and prevention was notified of a suspected case of hantavirus pulmonary syndrome (hps) in a man aged 70 years with no recent out-of-state travel. the maine resident went to a community hospital in early april with a 5-day history of fatigue, decreased appetite, weakness, chills, myalgias, and progressive shortness of breath. on examination, he was hypoxic and tachypneic. the patient was admitted with laboratory evidence of acute renal insuf ... | 2011 | 21681176 |
| hantavirus ecology in rodent populations in three protected areas of argentina. | in this study, we identified hantavirus genotypes and their reservoirs and evaluated the spatial and temporal distribution of the virus in rodent population in three protected areas of argentina over 3ôçâyears (2007-2010). a total of 837 rodents were captured with an effort of 22ôçâ117 trap-nights. we detected the genotype lechiguanas in oligoryzomys nigripes and o.-áflavescens and pergamino in akodon azarae. there was no correlation between seroprevalence and trap success of the host. the propo ... | 2011 | 21733047 |
| detection of dobrava-belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and sybr green-based real-time reverse transcriptase-polymerase chain reaction. | dobrava (dobv) hantaviruses belong to the genus hantavirus, family bunyaviridae, and are carried by yellow-necked and striped field mice. the goal of this study was to detect dobv using serological and genetic methods in apodemus rodents in hungary and in northern croatia. during the study period, a total of 125 apodemus sp. (67 a. agrarius, 58 a. flavicollis) were tested for the presence of hantaviruses, and 21 rodents (17%) were positive by rrt-pcr and/or elisa. we conclude that the prevalence ... | 2011 | 21573689 |
| phylogenetic analysis of puumala virus subtype bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein. | puumala virus (puuv) is the predominant hantavirus species in germany causing large numbers of mild to moderate cases of haemorrhagic fever with renal syndrome (hfrs). during an outbreak in south-east germany in 2004 a novel puuv subtype designated bavaria was identified as the causative agent of hfrs in humans [1]. here we present a molecular characterization of this puuv strain by investigating novel partial and almost entire nucleocapsid (n) protein-encoding small (s-) segment sequences and p ... | 2011 | 21598005 |
| pulmonary high-resolution computed tomography findings in nephropathia epidemica. | purpose: to evaluate lung high-resolution computed tomography (hrct) findings in patients with puumala hantavirus-induced nephropathia epidemica (ne), and to determine if these findings correspond to chest radiograph findings. materials and methods: hrct findings and clinical course were studied in 13 hospital-treated ne patients. chest radiograph findings were studied in 12 of them. results: twelve patients (92%) showed lung parenchymal abnormalities in hrct, while only 8 had changes in their c ... | 2011 | 21600717 |
| Incidence rate for hantavirus infections without pulmonary syndrome, Panama. | During 2001-2007, to determine incidence of all hantavirus infections, including those without pulmonary syndrome, in western Panama, we conducted 11 communitywide surveys. Among 1,129 persons, antibody prevalence was 16.5%-60.4%. Repeat surveys of 476 found that patients who seroconverted outnumbered patients with hantavirus pulmonary syndrome by 14 to 1. | 2011 | 22000376 |
| Rodent-Borne Hantaviruses in Cambodia, Lao PDR, and Thailand. | In order to evaluate the circulation of hantaviruses present in southeast Asia, a large scale survey of small mammal species was carried out at seven main sites in the region (Cambodia, Lao People's Democratic Republic, and Thailand). Small scale opportunistic trapping was also performed at an eighth site (Cambodia). Using a standard IFA test, IgG antibodies reacting to Hantaan virus antigens were detected at six sites. Antibody prevalence at each site varied from 0 to 5.6% with antibodies detec ... | 2011 | 22124701 |
| a possible case of hantavirus infection in a borneo orangutan and its conservation implication. | natural infection of hantavirus in orangutans has never been reported. | 2011 | 20722771 |
| hantavirus outbreak in western europe: reservoir host infection dynamics related to human disease patterns. | within europe, puumala virus (puuv) is the causal agent of nephropathia epidemica (ne) in humans, a zoonotic disease with increasing significance in recent years. in a region of belgium with a historically high incidence of ne, bank voles (the puuv reservoir hosts), were monitored for puuv igg antibody prevalence in nine study sites before, during, and after the highest ne outbreak recorded in belgium in 2005. we found that the highest numbers of puuv igg-positive voles coincided with the peak o ... | 2011 | 20450527 |
| environmental risk factors for haemorrhagic fever with renal syndrome in a french new epidemic area. | summaryin france, haemorrhagic fever with renal syndrome (hfrs) is endemic along the belgian border. however, this rodent-borne zoonosis caused by the puumala virus has recently spread south to the franche-comté region. we investigated the space-time distribution of hfrs and evaluated the influence of environmental factors that drive the hantavirus reservoir abundance and/or the disease transmission in this area. a scan test clearly indicated space-time clustering, highlighting a single-year (20 ... | 2011 | 20822577 |
| prospective study on ocular findings in acute puumala hantavirus infection in hospitalised patients. | to appraise the ocular manifestations of puumala hantavirus evoked haemorrhagic fever with renal syndrome nephropathia epidemica (ne) and to clarify the mechanisms of ocular changes in the largest series of patients examined to date. | 2011 | 20679079 |
| puumala virus infection: radiologic findings. | a 33-year-old male patient was admitted to our nephrology department with rapidly deteriorating general health, fever, respiratory difficulties, and acute renal failure. computed tomography of the thorax revealed interstitial edema with thickening of the interlobular septa, peribronchial cuffing, ground-glass opacities, and small pleural and pericardial effusions. polymerase chain reaction tests verified puumala virus infection. the patient recovered with supportive treatment. hantavirus infecti ... | 2011 | 20736852 |
| non-human primates in outdoor enclosures: risk for infection with rodent-borne hantaviruses. | different species of non-human primates have been exploited as animal disease models for human hantavirus infections. to study the potential risk of natural hantavirus infection of non-human primates, we investigated serum samples from non-human primates of three species living in outdoor enclosures of the german primate center (gpc), göttingen, located in a hantavirus endemic region of central germany. for that purpose we used serological assays based on recombinant antigens of the bank vole (m ... | 2011 | 20727685 |
| hantavirus protein interactions regulate cellular functions and signaling responses. | rodent-borne pathogenic hantaviruses cause two severe and often lethal zoonotic diseases: hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus cardiopulmonary syndrome (hcps) in the americas. currently, no us fda-approved therapeutics or vaccines are available for hfrs/hcps. infections with hantaviruses are not lytic, and it is currently not known exactly why infections in humans cause disease. a better understanding of how hantaviruses interfere with normal cell functions and ... | 2011 | 21171876 |
| [nephropathia epidemica caused by puumala hantavirus infection: a case report from the outpatient sector]. | a 42-year-old man without pre-existing health problems presented to his family practitioner in baden-württemberg with fever (up to 39˚ c for two days), headache, abdominal pain, back pain and aching limbs. ten days before, he had done heavy gardening work. he felt diffuse pain on abdominal palpation. the physical examination of the skin, lymph nodes, the heart, the lungs and the oral mucosa was unremarkable. | 2010 | 21140328 |
| evidence of human hantavirus infection and zoonotic investigation of hantavirus prevalence in rodents in western java, indonesia. | abstract during febrile surveillance in the western java city of bandung, indonesia, a patient with clinical symptoms consistent with hantavirus infection was found to have elevated titers of hantavirus-specific immunoglobulin m (igm) and igg antibodies. a subsequent epizoological investigation demonstrated a higher prevalence of hantavirus igg antibodies in rodents trapped in the vicinity of the patient's home compared with rodents from a control area (13.2% vs. 4.7%, p = 0.036). the old world ... | 2010 | 21142967 |
| small mammal-associated zoonoses. | 2010 | 20795915 | |
| hantaviruses and their hosts in europe: reservoirs here and there, but not everywhere? | five hantaviruses are known to circulate among rodents in europe, and at least two among insectivores. four (dobrava, saaremaa, seoul, and puumala [puuv] viruses) are clearly associated with hemorrhagic fever with renal syndrome (hfrs). puuv, the most common etiological agent of hfrs in europe, is carried by the bank vole (myodes glareolus), one of the most widespread and abundant mammal species in europe. this host-virus system is among hantaviruses also the most studied one in europe. however, ... | 2010 | 20795916 |
| seroprevalence of hantavirus antibodies among chronic hemodialysis patients in the czech republic. | a total of 348 serum samples were collected from 301 hemodialysis patients with chronic renal failure of four healthcare settings in western bohemia. the sera were screened for the presence of hantavirus antibodies using elisa kits (progen biotechnik gmbh) with hantaan and puumala antigens. specific anti-puumala antibodies were detected in five patients (1.7%). although hantaviruses are known to cause primarily acute renal damage (interstitial nephritis) in eurasia, chronic effects of hantavirus ... | 2010 | 21105569 |
| circulation of hantaviruses in the influence area of the cuiabá-santarém highway. | we describe evidence of circulation of hantaviruses in the influence area of the santarém-cuiabá highway (br-163) in the brazilian amazon through the prevalence of specific antibodies against hantaviruses in inhabitants living in four municipalities of this area: novo progresso (2.16%) and trairão (4.37%), in state of pará (pa), and gua-rantã do norte (4.74%) and marcelândia (9.43%), in state of mato grosso. we also demonstrate the ongoing association between castelo dos sonhos virus (casv) and ... | 2010 | 20835614 |
| hantavirus pulmonary syndrome in argentina, 1995-2008. | we report a large case series of hantavirus pulmonary syndrome (hps) in argentina that was confirmed by laboratory results from 1995 through 2008. the geographic and temporal distribution of cases by age, sex, fatality rate, and risk factors for hps was analyzed. a total of 710 cases were unequally distributed among 4 of the 5 argentine regions. different case-fatality rates were observed for each affected region, with a maximum rate of 40.5%. the male-to-female ratio for hps case-patients was 3 ... | 2010 | 21122213 |
| geographical range of rio mamoré virus (family bunyaviridae, genus hantavirus) in association with the small-eared pygmy rice rat (oligoryzomys microtis). | hantavirus htn.007 was originally isolated from a small-eared pygmy rice rat (oligoryzomys microtis) captured in northeastern peru. the results of analyses of nucleotide and amino acid sequence data in this study indicated that htn.007 is a strain of rio mamoré virus (riomv) which is enzootic in small-eared pygmy rice rat populations in bolivia. as such, the results of this study extend our knowledge of the geographical range of riomv and support the notion that the small-eared pygmy rice rat is ... | 2010 | 20687859 |
| natural history of sin nombre virus infection in deer mice in urban parks in oregon. | sin nombre virus (snv), one of at least 45 hantaviruses described worldwide, is hosted by the deer mouse, peromyscus maniculatus, a common species throughout most of north america. herein, we describe general life-history characteristics of deer mice and the ways in which these factors relate to the incidence of snv infections among populations of this host species in and around portland, oregon. in total, 3,175 deer mice were captured from october 2002 to september 2005. transmission of snv app ... | 2010 | 20688636 |
| long-term patterns of immune investment by wild deer mice infected with sin nombre virus. | immunocompetence of animals fluctuates seasonally, however, there is little consensus on the cause of these fluctuations. some studies have suggested that these patterns are influenced by changes in reproductive condition, whereas others have suggested that differences result from seasonal variations in energy expenditures. the objective of our study was to examine these contrasting views of immunity by evaluating seasonal patterns of immune response and reproduction in wild populations of deer ... | 2010 | 20695811 |
| hantavirus outbreak associated with laboratory rats in yunnan, china. | an outbreak of hemorrhagic fever with renal syndrome occurred among students in a college (college a) in kunming, yunnan province, china in 2003. subsequent investigations revealed the presence of hantavirus antibodies and antigens in laboratory rats at college a and two other institutions. hantavirus antibodies were detected in 15 additional individuals other than the index case in these three locations. epidemiologic data indicated that the human infections were a result of zoonotic transmissi ... | 2010 | 20380897 |
| epidemic viral pneumonia. | two recent viral epidemics producing pneumonitis (severe acute respiratory syndrome and pandemic influenza a h1n1) have highlighted the potential for viral infections to cause respiratory failure with a significant risk of mortality. this review describes these epidemics and other causes of epidemic viral pneumonia. | 2010 | 20087179 |
| travellers and viral haemorrhagic fevers: what are the risks? | viral haemorrhagic fevers (vhf) are caused by zoonotic viral infections transmitted to humans directly or by ticks or mosquitoes. the overall risk to travellers is conservatively estimated at <1 in 1 million travel episodes to african countries where infection is present, and febrile patients returning from these countries are at least 1000 times more likely to have malaria than lassa fever or another vhf. no cases have been reported in fellow travellers exposed to a travelling case and only one ... | 2010 | 20705436 |
| pathogenic hantaviruses andes virus and hantaan virus induce adherens junction disassembly by directing vascular endothelial cadherin internalization in human endothelial cells. | hantaviruses infect endothelial cells and cause 2 vascular permeability-based diseases. pathogenic hantaviruses enhance the permeability of endothelial cells in response to vascular endothelial growth factor (vegf). however, the mechanism by which hantaviruses hyperpermeabilize endothelial cells has not been defined. the paracellular permeability of endothelial cells is uniquely determined by the homophilic assembly of vascular endothelial cadherin (ve-cadherin) within adherens junctions, which ... | 2010 | 20463083 |
| hantavirus-like particles generated in cho cells induce specific immune responses in c57bl/6 mice. | a safe and effective hantavirus vaccine is highly desirable since hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, hfrs). virus-like particles (vlps) displaying functional viral proteins could provide effective vaccines against a few viruses, but their ability to induce hantavirus-specific immune response has not been adequately investigated. to measure the immunogenicity of hantaan virus-like particles (htn-vlps) vaccine, ... | 2010 | 20433802 |
| the severity of acute puumala hantavirus infection does not predict the long-term outcome of patients. | we have found greater urinary protein excretion and higher glomerular filtration rate (gfr) and blood pressure in patients 6 years after acute nephropathia epidemica (ne) compared with seronegative controls. the present aim was to establish whether the long-term outcome is determined by the severity of acute illness. | 2010 | 20502044 |
| relationship of human behavior within outbuildings to potential exposure to sin nombre virus in western montana. | sin nombre virus (snv) causes hantavirus cardiopulmonary syndrome (hcps) in humans. transmission of snv among the deer mouse (peromyscus maniculatus) host predominates during spring and summer, and is greater in peridomestic than sylvan settings where, protected from uv light, snv may survive longer. incidence of hcps reflects these times and settings and is associated with inhalation of mouse excreta. little is known, however, about how human use of outbuildings contributes to potential exposur ... | 2010 | 20508970 |
| sin nombre virus-specific immunoglobulin m and g kinetics in hantavirus pulmonary syndrome and the role played by serologic responses in predicting disease outcome. | sin nombre virus (snv) is the primary cause of hantavirus pulmonary syndrome (hps) in the united states. although other studies have demonstrated a possible association between neutralizing antibody titers and the severity of hps, the exact nature of serologic responses and their association with outcomes have not been fully characterized. | 2010 | 20521946 |
| ecology of hantavirus in a changing world. | hantavirus is a genus of virus represented by 45 different species and is hosted by small mammals, predominantly rats and mice. roughly, half of all hantaviruses cause diseases in humans that vary in morbidity from mild to severe. the natural and anthropogenic changes occurring in the environment appear to be impacting the ecology of hantaviruses and their natural hosts as well as the incidence of hantaviral diseases in humans. although such studies are limited at this time, there is evidence th ... | 2010 | 20536819 |
| hantavirus infections in fluctuating host populations: the role of maternal antibodies. | infected females may transfer maternal antibodies (matabs) to their offspring, which may then be transiently protected against infections the mother has encountered. however, the role of maternal protection in infectious disease dynamics in wildlife has largely been neglected. here, we investigate the effects of puumala hantavirus (puuv)-specific matabs on puuv dynamics, using 7 years' data from a cyclic bank vole population in finland. for the first time to our knowledge, we partition seroposit ... | 2010 | 20591866 |
| acute undifferentiated febrile illness in adult hospitalized patients: the disease spectrum and diagnostic predictors - an experience from a tertiary care hospital in south india. | local prevalences of individual diseases influence the prioritization of the differential diagnoses of a clinical syndrome of acute undifferentiated febrile illness (afi). this study was conducted in order to delineate the aetiology of afi that present to a tertiary hospital in southern india and to describe disease-specific clinical profiles. an 1-year prospective, observational study was conducted in adults (age >16 years) who presented with an undifferentiated febrile illness of duration 5-21 ... | 2010 | 20870680 |
| central nervous system-related symptoms and findings are common in acute puumala hantavirus infection. | puumala hantavirus (puuv) causes a hemorrhagic fever with renal syndrome (hfrs) also called nephropathia epidemica (ne). recent case reports and retrospective studies suggest that ne may damage the pituitary gland. based on these observations, our goal was to explore the nature of this complication prospectively. | 2010 | 20545485 |
| development of a lentiviral vector system to study the role of the andes virus glycoproteins. | to infect target cells, enveloped viruses use their virion surface proteins to direct cell attachment and subsequent entry via virus-cell membrane fusion. how hantaviruses enter cells has been largely unexplored. to study early steps of andes virus (andv) cell infection, a lentiviral vector system was developed based on a simian immunodeficiency virus (siv) vector pseudotyped with the andv-gn/gc envelope glycoproteins. the incorporation of gn and gc onto siv-derived vector particles was assessed ... | 2010 | 20619306 |
| [hantavirus]. | 2010 | 20942073 | |
| the relative abundance of deer mice with antibody to sin nombre virus corresponds to the occurrence of hantavirus pulmonary syndrome in nearby humans. | abstract sin nombre virus (snv) is the principal cause of hantavirus pulmonary syndrome (hps) in the united states and deer mice (peromyscus maniculatus) are its principal rodent host, and thus the natural cycle of the virus is related to the occurrence of hps. prevalence of rodent infection appears to be associated with fluctuations in deer mouse populations and, indirectly, with timing and amount of precipitation, a complex of biologic events. given that rodent population abundances fluctuate, ... | 2010 | 20954865 |
| in vivo kinetics and biodistribution of a hantaan virus dna vaccine after intramuscular injection in mice. | to study the kinetics in vivo of a hantaan virus dna vaccine, we constructed a fusion dna vaccine, pegfp/s, by cloning the s segment of hantavirus into the vector, pegfp-c1, which encodes green fluorescent protein egfp. in this report, we provide evidence that pegfp/s was distributed and persistently expressed for more than 60 days in several organs after inoculation. our findings suggest that the persistent immune responses induced by a hantaan virus dna vaccine are likely due to the plasmid pe ... | 2010 | 20960291 |
| satellite derived forest phenology and its relation with nephropathia epidemica in belgium. | the connection between nephropathia epidemica (ne) and vegetation dynamics has been emphasized in recent studies. changing climate has been suggested as a triggering factor of recently observed epidemiologic peaks in reported ne cases. we have investigated whether there is a connection between the ne occurrence pattern in belgium and specific trends in remotely sensed phenology parameters of broad-leaved forests. the analysis of time series of the modis enhanced vegetation index revealed that ch ... | 2010 | 20644685 |
| pathogenic landscapes: interactions between land, people, disease vectors, and their animal hosts. | landscape attributes influence spatial variations in disease risk or incidence. we present a review of the key findings from eight case studies that we conducted in europe and west africa on the impact of land changes on emerging or re-emerging vector-borne diseases and/or zoonoses. the case studies concern west nile virus transmission in senegal, tick-borne encephalitis incidence in latvia, sandfly abundance in the french pyrenees, rift valley fever in the ferlo (senegal), west nile fever and t ... | 2010 | 20979609 |
| temporal and spatial host abundance and prevalence of andes hantavirus in southern argentina. | andes virus (and) is a hantavirus hosted by the sigmodontine rodent oligoryzomys longicaudatus in southern argentina, where it is responsible for most cases of hantavirus pulmonary syndrome (hps). our study provides data about the spatial variation in abundance of the rodent host of and hantavirus. we report results of a longitudinal study performed in a locality of the andean region of chubut province. from november 2003 (spring) to july 2006 (winter), o. longicaudatus was the most common speci ... | 2010 | 20645121 |
| syndromic surveillance: etiologic study of acute febrile illness in dengue suspicious cases with negative serology. brazil, federal district, 2008. | with the aim of identifying the etiology of acute febrile illness in patients suspected of having dengue, yet with non reagent serum, a descriptive study was conducted with 144 people using secondary serum samples collected during convalescence. the study was conducted between january and may of 2008. all the exams were re-tested for dengue, which was confirmed in 11.8% (n = 17); the samples that remained negative for dengue (n = 127) were tested for rubella, with 3.9% (n = 5) positive results. ... | 2010 | 21049226 |
| [hemorrhagic fever with renal syndrome during pregnancy: case report]. | hemorrhagic fever with renal syndrome (hfwrs) is an acute infectious disease with abrupt onset, high fever, renal failure and frequent hemorragies. hfwrs during the pregnancy has relatively low occurrence, with only few described cases mostly in the region of the former soviet union. although, according to the data from the literature, the disease is less severe during the pregnancy, the eclampsy, stillbirth and fetal infection were also described. | 2010 | 21053473 |
| [construction and immunogenic study of recombinant adenovirus containing chimeric gene g2s0.7 and ctl epitopes of hantaan virus]. | to express g2 fragment of m segment and 0.7 kb fragment of s segment and several ctl epitopes of s segment in adenovirus expression system and investigate the immunological properties of hantaan virus chimeric gene. | 2010 | 21055343 |
| occupation-related respiratory infections revisited. | occupational pulmonary infectious diseases include tuberculosis (tb) and many viral pathogens, including influenza, coronavirus (severe acute respiratory syndrome or sars), varicella, respiratory syncytial virus, and hantavirus. this review focuses on tb, influenza, and sars, because the published literature is extensive for these 3 infections. the lessons from these 3 are relevant for all nosocomial pulmonary infectious diseases. | 2010 | 20674797 |
| structural studies of hantaan virus. | hantaan virus is the prototypic member of the hantavirus genus within the family bunyaviridae and is a causative agent of the potentially fatal hemorrhagic fever with renal syndrome. the bunyaviridae are a family of negative-sense rna viruses with three-part segmented genomes. virions are enveloped and decorated with spikes derived from a pair of glycoproteins (gn and gc). here, we present cryo-electron tomography and single-particle cryo-electron microscopy studies of hantaan virus virions. we ... | 2010 | 21068243 |
| andes virus disrupts the endothelial cell barrier by induction of vascular endothelial growth factor and downregulation of ve-cadherin. | hantavirus pulmonary syndrome (hps) and hemorrhagic fever with renal syndrome (hfrs) are severe diseases associated with hantavirus infection. high levels of virus replication occur in microvascular endothelial cells but without a virus-induced cytopathic effect. however, virus infection results in microvascular leakage, which is the hallmark of these diseases. ve-cadherin is a major component of adherens junctions, and its interaction with the vascular endothelial growth factor (vegf) receptor, ... | 2010 | 20810734 |
| tracking hantavirus nucleocapsid protein using intracellular antibodies. | hantavirus nucleocapsid (n) protein is a multifunctional viral macromolecule involved in multiple stages of the viral replication cycle. the intracellular trafficking of n protein during virus assembly remains unclear. | 2010 | 21092325 |
| small molecule inhibitors of hantavirus infection. | hantaviruses use α(v)β(3) integrins on the surface of human host cells as a gateway to invasion, hence compounds that target this receptor may be used as antiviral agents. to accomplish this aim, new peptidomimetic compounds were selected based on similarity to a cyclic peptide known to bind the α(v)β(3) receptor. this first round of biological screening identified peptidomimetic molecules which were effective hantavirus inhibitors in the low micromolar range, two thousand times more potent than ... | 2010 | 20951038 |
| confirmation of choclo virus as the cause of hantavirus cardiopulmonary syndrome and high serum antibody prevalence in panama. | choclo virus (chov) was described in sigmodontine rodents, oligoryzomys fulvescens, and humans during an outbreak of hantavirus cardiopulmonary syndrome (hcps) in 1999-2000 in western panama. although hcps is rare, hantavirus-specific serum antibody prevalence among the general population is high suggesting that chov may cause many mild or asymptomatic infections. the goals of this study were to confirm the role of chov in hcps and in the frequently detected serum antibody and to establish the p ... | 2010 | 20648614 |
| the 3' untranslated region of the andes hantavirus small mrna functionally replaces the poly(a) tail and stimulates cap-dependent translation initiation from the viral mrna. | in the process of translation of eukaryotic mrnas, the 5' cap and the 3' poly(a) tail interact synergistically to stimulate protein synthesis. unlike its cellular counterparts, the small mrna (smrna) of andes hantavirus (andv), a member of the bunyaviridae, lacks a 3' poly(a) tail. here we report that the 3' untranslated region (3'utr) of the andv smrna functionally replaces a poly(a) tail and synergistically stimulates cap-dependent translation initiation from the viral mrna. stimulation of tra ... | 2010 | 20660206 |
| genetic reassortment between high-virulent and low-virulent dobrava-belgrade virus strains. | the tri-segmented rna genome of hantaviruses facilitates genetic reassortment by segment swapping when cells are co-infected with different virus strains. we found efficient in vitro reassortment between members of two different genetic lineages of the dobrava-belgrade virus species, the weakly virulent dobv-aa and highly virulent dobv-af. in all reassortants, s and l segments originated from the same parental strain, and only the m segment was exchanged. to identify functional differences betwe ... | 2010 | 20734125 |
| hormonal deficiencies during and after puumala hantavirus infection. | previous reports have described panhypopituitarism associated with severe cases of hemorrhagic fever with renal syndrome (hfrs), but the prevalence of hormonal deficiencies after nephropathia epidemica (ne), a milder form of hfrs, has not been studied. this study was conducted in order to determine the prevalence of hormonal defects in patients with acute ne and during long-term follow-up. fifty-four patients with serologically confirmed acute ne were examined by serum hormonal measurements duri ... | 2010 | 20397036 |
| [construction and identification of a new type of recombinant adenovirus containing s0.7 gene of hantavirus]. | to construct a adenovirus vector containing the 0.7 kb fragment of s gene of hantavirus, cag promoter, and wpre (mrna-stabilizing post-transcriptional regulatory element from the woodchuck hepatitis virus). | 2010 | 20423645 |
| [dynamics of hantavirus detection in the excretory organs of apodemus mice and its relation to epidemic manifestations of hantavirus infection]. | this paper presents data on the detection of hantavirus in the excretory organs of apodemus mice which are natural carriers of pathogenic hantaviral serotypes in the primorye region. acute infection periods were detected in naturally infected rodents with the highest hantavirus dissemination to the environment in different phases of rodent population cycles. there was a relationship between the rise of morbidity and the seasonal manifestation of hemorrhagic fever with renal syndrome morbidity an ... | 2010 | 20455471 |
| diagnosis of hantavirus infection in humans and rodents in ribeirão preto, state of são paulo, brazil. | hantavirus pulmonary and cardiovascular syndrome (hpcs) is an emerging serious disease in the americas. hantaviruses (bunyaviridae) are the causative agents of this syndrome and are mainly transmitted through inhalation of aerosols containing the excreta of wild rodents. in the ribeirão preto region (state of são paulo, brazil), hpcs has been reported since 1998, caused by the araraquara virus (arav), for which necromys lasiurus is the rodent reservoir. this study aimed to show diagnostic result ... | 2010 | 20802928 |
| models for the spread and persistence of hantavirus infection in rodents with direct and indirect transmission. | hantavirus, a zoonotic disease carried by wild rodents, is spread among rodents via direct contact and indirectly via infected rodent excreta in the soil. spillover to humans is primarily via the indirect route through inhalation of aerosolized viral particles. rodent-hantavirus models that include direct and indirect transmission and periodically varying demographic and epidemiological parameters are studied in this investigation. two models are analyzed, a nonautonomous system of differential ... | 2010 | 20462286 |
| comprehensive panel of real-time taqman polymerase chain reaction assays for detection and absolute quantification of filoviruses, arenaviruses, and new world hantaviruses. | viral hemorrhagic fever is caused by a diverse group of single-stranded, negative-sense or positive-sense rna viruses belonging to the families filoviridae (ebola and marburg), arenaviridae (lassa, junin, machupo, sabia, and guanarito), and bunyaviridae (hantavirus). disease characteristics in these families mark each with the potential to be used as a biological threat agent. because other diseases have similar clinical symptoms, specific laboratory diagnostic tests are necessary to provide the ... | 2010 | 20439981 |
| infection of in vivo differentiated human mast cells with hantaviruses. | increased vascular permeability is a key feature of the pathological symptoms caused by hantaviruses. here, we analysed the interaction between hantaviruses and mast cells, which regulate vascular homeostasis. in highly purified human skin mast cells increasing amounts of hantaan (htnv) and, to a lower extent, prospect hill (phv) virions were produced. replication was confirmed by the production of viral plus-strand rna as determined by a virus strand-specific rt-pcr. phv but not htnv elicited e ... | 2010 | 20071485 |
| models for the spread and persistence of hantavirus infection in rodents with direct and indirect transmission. | hantavirus, a zoonotic disease carried by wild rodents, is spread among rodents via direct contact and indirectly via infected rodent excreta in the soil. spillover to humans is primarily via the indirect route through inhalation of aerosolized viral particles. rodent-hantavirus models that include direct and indirect transmission and periodically varying demographic and epidemiological parameters are studied in this investigation. two models are analyzed, a nonautonomous system of differential ... | 2010 | 20104956 |
| puumala hantavirus and myodes glareolus in northern europe: no evidence of co-divergence between genetic lineages of virus and host. | the genus hantavirus (family bunyaviridae) includes negative-strand rna viruses that are carried by persistently infected rodent and insectivore species. puumala virus (puuv), carried by bank voles (myodes glareolus), is a pathogenic hantavirus that causes outbreaks of mild haemorrhagic fever with renal syndrome across europe. in northern europe, puuv is represented by several genetic lineages that are maintained by distinct phylogroups of bank voles. the present study describes sequences of new ... | 2010 | 20107019 |
| pathology of black creek canal virus infection in juvenile hispid cotton rats (sigmodon hispidus). | the purpose of this study was to assess the effect of inoculum dose on the pathogenesis of black creek canal virus (bccv) infection in the hispid cotton rat (sigmodon hispidus), the principal host of bccv. no sign of illness was observed in any of the 52 juvenile hispid cotton rats inoculated with 3.1, 1.1, -0.9, or -2.9 log(10) median infectious doses(veroe6) (id(50-veroe6)) of bccv and euthanized on day 9, 18, 27, or 54 postinoculation (pi). analysis of virus assay and serological data indicat ... | 2010 | 20455779 |
| sin nombre virus infection in field workers, colorado, usa. | we report 2 cases of sin nombre virus (snv) infection in field workers, possibly contracted through rodent bites. screening for antibodies to snv in rodents trapped in 2 seasons showed that 9.77% were seropositive. quantitative real-time pcr showed that 2 of 79 deer mice had detectable titers of snv rna. | 2010 | 20113567 |