Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| longitudinal analysis of the human t cell response during acute hantavirus infection. | longitudinal studies of t cell immune responses during viral infections in humans are essential for our understanding of how effector t cell responses develop, clear infection, and provide long-lasting immunity. here, following an outbreak of a puumala hantavirus infection in the human population, we longitudinally analyzed the primary cd8 t cell response in infected individuals from the first onset of clinical symptoms until viral clearance. a vigorous cd8 t cell response was observed early fol ... | 2011 | 21795350 |
| t cells and pathogenesis of hantavirus cardiopulmonary syndrome and hemorrhagic fever with renal syndrome. | we previously hypothesized that increased capillary permeability observed in both hantavirus cardiopulmonary syndrome (hcps) and hemorrhagic fever with renal syndrome (hfrs) may be caused by hantavirus-specific cytotoxic t cells attacking endothelial cells presenting viral antigens on their surface based on clinical observations and in vitro experiments. in hcps, hantavirus-specific t cell responses positively correlated with disease severity. in hfrs, in one report, contrary to hcps, t cell res ... | 2011 | 21994770 |
| hla associated hemorrhagic fever with renal syndrom disease progression in slovenian patients. | mhc class i and class ii genes regulate the balance between appropriate aggressive responses and invading pathogens while minimising the destruction of host tissue. several studies have shown that in hfrs patients the disease outcome is determined by a complex interaction between the virus, immunopathologic and human genetic factors. in slovenia the severity of the disease caused by puuv is significantly lower than that of hfrs due to dobv. we have determined 23 different hla-b and 12 different ... | 2011 | 21775516 |
| young male patients are at elevated risk of developing serious central nervous system complications during acute puumala hantavirus infection. | abstract: | 2011 | 21838931 |
| a possible case of hantavirus infection in a borneo orangutan and its conservation implication. | natural infection of hantavirus in orangutans has never been reported. | 2011 | 20722771 |
| non-human primates in outdoor enclosures: risk for infection with rodent-borne hantaviruses. | different species of non-human primates have been exploited as animal disease models for human hantavirus infections. to study the potential risk of natural hantavirus infection of non-human primates, we investigated serum samples from non-human primates of three species living in outdoor enclosures of the german primate center (gpc), göttingen, located in a hantavirus endemic region of central germany. for that purpose we used serological assays based on recombinant antigens of the bank vole (m ... | 2011 | 20727685 |
| puumala virus infection: radiologic findings. | a 33-year-old male patient was admitted to our nephrology department with rapidly deteriorating general health, fever, respiratory difficulties, and acute renal failure. computed tomography of the thorax revealed interstitial edema with thickening of the interlobular septa, peribronchial cuffing, ground-glass opacities, and small pleural and pericardial effusions. polymerase chain reaction tests verified puumala virus infection. the patient recovered with supportive treatment. hantavirus infecti ... | 2011 | 20736852 |
| environmental risk factors for haemorrhagic fever with renal syndrome in a french new epidemic area. | summaryin france, haemorrhagic fever with renal syndrome (hfrs) is endemic along the belgian border. however, this rodent-borne zoonosis caused by the puumala virus has recently spread south to the franche-comté region. we investigated the space-time distribution of hfrs and evaluated the influence of environmental factors that drive the hantavirus reservoir abundance and/or the disease transmission in this area. a scan test clearly indicated space-time clustering, highlighting a single-year (20 ... | 2011 | 20822577 |
| specific humoral reaction of hemorrhagic fever with renal syndrome (hfrs) patients in china to recombinant nucleocapsid proteins from european hantaviruses. | hemorrhagic fever with renal syndrome (hfrs) is endemic in east asia and europe. this study was initiated to investigate the reactivity of antibodies in sera of chinese hfrs patients with the recombinant nucleocapsid proteins (rnps) of hantaan virus (htnv), dobrava-belgrade virus (dobv), and puumala virus (puuv), which are the prevalent hantavirus strains in europe. forty-eight pairs of acute and convalescent sera were collected from hfrs patients in hubei, china (1985-2002) and tested by indire ... | 2011 | 21222012 |
| time to revise the paradigm of hantavirus syndromes? hantavirus pulmonary syndrome caused by european hantavirus. | hantaviruses have previously been recognised to cause two separate syndromes: hemorrhagic fever with renal syndrome in eurasia, and hantavirus pulmonary syndrome (hps) in the americas. however, increasing evidence suggests that this dichotomy is no longer fruitful when recognising human hantavirus disease and understanding the pathogenesis. herein are presented three cases of severe european puumala hantavirus infection that meet the hps case definition. the clinical and pathological findings we ... | 2011 | 21234633 |
| interview with pedro fernando da costa vasconcelos, md, phd. interview by vicki glaser. | 2011 | 21261512 | |
| hantavirus infection during a stay in a mountain hut in northern slovakia. | hantaviruses in europe cause human hemorrhagic fever with renal syndrome (hfrs) with various degree of severity. the most severe form is caused by the dobrava/belgrade virus (dobv), associated with the rodent apodemus flavicollis. during the last decade cases of infection caused by dobv have been reported in central europe. the present study is a report on two czech patients with severe hfrs who were infected during their stay in a mountain hut in northern slovakia. the two patients, combined wi ... | 2011 | 21264871 |
| hantavirus infection in istanbul, turkey. | 2011 | 21291612 | |
| genetic detection of dobrava/belgrade virus, bulgaria. | 2011 | 21291615 | |
| concomitant influence of helminth infection and landscape on the distribution of puumala hantavirus in its reservoir, myodes glareolus. | puumala virus, the agent of nephropathia epidemica (ne), is the most prevalent hantavirus in europe. the risk for human infection seems to be strongly correlated with the prevalence of puumala virus (puuv) in populations of its reservoir host species, the bank vole myodes glareolus. in humans, the infection risks of major viral diseases are affected by the presence of helminth infections. we therefore proposed to analyse the influence of both helminth community and landscape on the prevalence of ... | 2011 | 21303497 |
| genetic diversity of imjin virus in the ussuri white-toothed shrew (crocidura lasiura) in the republic of korea, 2004-2010. | recently, imjin virus (mjnv), a genetically distinct hantavirus, was isolated from lung tissues of the ussuri white-toothed shrew (crocidura lasiura) captured near the demilitarized zone in the republic of korea. to clarify the genetic diversity of mjnv, partial m- and l-segment sequences were amplified from lung tissues of 12 of 37 (32.4%) anti-mjnv igg antibody-positive ussuri white-toothed shrews captured between 2004 and 2010. a 531-nucleotide region of the m segment (coordinates 2,255 to 2, ... | 2011 | 21303516 |
| [bilateral acute angle-closure glaucoma due to an infection with hantavirus]. | a poor general condition of patients with hemorrhagic fever and elevated creatinine should raise suspicion of a systemic infection with hantavirus. this can often also cause ocular changes. above all, changes of intraocular pressure, in our case bilateral acute angle-closure glaucoma, due to edema and hemorrhage in the ciliary body result in anterolateral rotation of the iris-lens diaphragm with closure of the chamber angle. it can also cause a transient myopia, and intraretinal hemorrhages can ... | 2011 | 21327947 |
| delayed density-dependent prevalence of sin nombre virus infection in deer mice (peromyscus maniculatus) in central and western montana. | understanding how transmission of zoonoses takes place within reservoir populations, such as sin nombre virus (snv) among deer mice (peromyscus maniculatus), is important in determining the risk of exposure to other hosts, including humans. in this study, we examined the relationship between deer mouse populations and the prevalence of antibodies to snv, a system where the effect of host population abundance on transmission is debated. we examined the relationship between abundance of deer mice ... | 2011 | 21269997 |
| rodent community structure and andes virus infection in sylvan and peridomestic habitats in northwestern patagonia, argentina. | modifications of natural habitat in peridomestic rural areas could affect original rodent community composition, diversity, and evenness. in zoonoses such as hantavirus pulmonary syndrome, the presence of a diverse community can dilute the impact of the principal reservoir, reducing risk to humans. the goal of this study was to examine rodent community composition, abundance of andes virus (andv) host (oligoryzomys longicaudatus), andv prevalence, and temporal variability associated with rural p ... | 2011 | 21332352 |
| procalcitonin in hantavirus infections. | in hantavirus infections levels of serum leukocytes or c-reactive protein are usually elevated to levels found in serious bacterial infections. however, procalcitonin in patients infected with hantavirus has not yet been discussed in the literature. a total of 29 adult patients with hantavirus infection, 30 with sepsis, and 19 with tick-borne encephalitis were included in this observational retrospective study. the median procalcitonin level in patients with hantavirus infection was 0.53 µg/l (r ... | 2011 | 21344982 |
| arenaviruses and hantaviruses: from epidemiology and genomics to antivirals. | the arenaviruses and hantaviruses are segmented genome rna viruses that are hosted by rodents. due to their association with rodents, they are globally widespread and can infect humans via direct or indirect routes of transmission, causing considerable human morbidity and mortality. nevertheless, despite their obvious and emerging importance as pathogens, there are currently no effective antiviral drugs (except ribavirin which proved effective against lassa virus) with which to treat humans infe ... | 2011 | 21356244 |
| using geographic information system-based ecologic niche models to forecast the risk of hantavirus infection in shandong province, china. | hemorrhagic fever with renal syndrome (hfrs) is an important public health problem in shandong province, china. in this study, we combined ecologic niche modeling with geographic information systems (gis) and remote sensing techniques to identify the risk factors and affected areas of hantavirus infections in rodent hosts. land cover and elevation were found to be closely associated with the presence of hantavirus-infected rodent hosts. the averaged area under the receiver operating characterist ... | 2011 | 21363991 |
| a novel hantavirus detected in yunnan red-backed vole (eothenomys miletus) in china. | rodents are the major natural reservoir of hantaviruses, which cause two main human zoonoses, haemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus pulmonary syndrome (hps) in the americas. surveillance of hantaviruses in rodents plays an important role in the prevention and control of hfrs and hps. in this study, small mammals were captured in an hfrs-endemic region, luxi county within the yunnan province of china, from the autumn of 2009 to the spring of 2010, and assessed fo ... | 2011 | 21402592 |
| seroepidemiological survey of hantavirus infection in healthy people in vallès occidental, barcelona. | hantaviruses are the etiological agents of hemorrhagic fever with renal syndrome in europe and asia, and hantavirus pulmonary syndrome in america. approximately 150,000 cases are reported annually worldwide. in spain, some hantavirus infection cases have been described. besides, rodents that have been described as hantavirus reservoirs are present. the aim of the present study was to determinate the seroprevalence of hantavirus in humans in the northeast of spain. | 2011 | 21417923 |
| recent advances in hantavirus molecular biology and disease. | hantaviruses are emerging zoonotic pathogens that belong to the bunyaviridae family. they have been classified as category a pathogens by cdc (centers for disease control and prevention). hantaviruses pose a serious threat to human health because their infection causes two highly fatal diseases, hemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps). these pathogens are transmitted to humans through aerosolized excreta of their infected rodent hosts. hantavir ... | 2011 | 21459193 |
| the c-terminal 42 residues of the tula virus gn protein regulate interferon induction. | hantaviruses primarily infect the endothelial cell lining of capillaries and cause two vascular permeability-based diseases. the ability of pathogenic hantaviruses to regulate the early induction of interferon determines whether hantaviruses replicate in endothelial cells. tula virus (tulv) and prospect hill virus (phv) are hantaviruses which infect human endothelial cells but fail to cause human disease. phv is unable to inhibit early interferon (ifn) responses and fails to replicate within hum ... | 2011 | 21367904 |
| prevalence of hantavirus infection in wild rodents from five provinces in korea, 2007. | hemorrhagic fever with renal syndrome (hfrs) is an infectious disease caused by hantaviruses of the family bunyaviridae. among them, hantaan virus (htnv) is most widely distributed in korea. the striped field mouse, apodemus agrarius, is the natural host of htnv in rural korea. we trapped 766 small mammals of three species (1 eothenomys regulus, 13 crocidura suaveolens, and 752 apodemus agrarius) in five provinces in korea from january to december 2007. we tested 542 rodent sera for htnv antibod ... | 2011 | 21441196 |
| viral infections in workers in hospital and research laboratory settings: a comparative review of infection modes and respective biosafety aspects. | to compare modes and sources of infection and clinical and biosafety aspects of accidental viral infections in hospital workers and research laboratory staff reported in scientific articles. | 2011 | 21497126 |
| structural characterization of the crimean-congo hemorrhagic fever virus gn tail provides insight into virus assembly. | the rna virus that causes the crimean congo hemorrhagic fever (cchf) is a tick-borne pathogen of the nairovirus genus, family bunyaviridae. unlike many zoonotic viruses that are only passed between animals and humans, the cchf virus can also be transmitted from human to human with an overall mortality rate approaching 30%. currently, there are no atomic structures for any cchf virus proteins nor for any nairovirus proteins. a critical component of the virus is the envelope gn glycoprotein, which ... | 2011 | 21507948 |
| the delicate pygmy rice rat (oligoryzomys delicatus) is the principal host of maporal virus (family bunyaviridae, genus hantavirus). | abstract choclo virus (chov) and maporal virus (mapv) are enzootic in panama and western venezuela, respectively. the results of previous studies suggested that the fulvous pygmy rice rat (oligoryzomys fulvescens) is the principal host of both viruses. the results of an analysis of nucleotide sequence data in this study indicated that the rodent associated with chov is the costa rican pygmy rice rat (oligoryzomys costaricensis) and that the rodent associated with mapv is the delicate pygmy rice ... | 2011 | 21548760 |
| maporal virus as a surrogate for pathogenic new world hantaviruses and its inhibition by favipiravir. | pathogenic hantaviruses geographically distributed in the old world cause haemorrhagic fever with renal syndrome (hfrs), whereas new world hantaviruses are the aetiological agents of hantavirus cardiopulmonary syndrome (hcps). ribavirin, a drug associated with toxicities, is presently indicated for treatment of hfrs, whereas treatment of the more frequently lethal hcps is limited to supportive care. because of the need for safe and effective antivirals to treat severe hantaviral infections, we e ... | 2011 | 21566265 |
| development and application of a flow cytometric potency assay for dna vaccines. | we have developed a rapid, reliable, and sensitive quantitative flow cytometric assay to measure the in vitro potency and stability of dna vaccines to be delivered either by particle-mediated epidermal delivery (pmed) or by electroporation. the method involves transfecting cells with test dna and comparing the measured antigen expression to that generated with expression from known quantities of reference material dna. the assay was adapted for performance under good laboratory practice (glp) gu ... | 2011 | 21219978 |
| hantavirus pulmonary syndrome: encephalitis caused by virus andes. | hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome (hps) are rodent-borne emerging diseases caused by members of the genus hantavirus, family bunyaviridae. some species of hantavirus may cause encephalitis, but this is the first report in andes virus associated to hps. | 2011 | 21240581 |
| serological evidence of thailand virus-related hantavirus infection among suspected leptospirosis patients in kandy, sri lanka. | a cross-sectional study was undertaken to determine the current prevalence of leptospirosis and hantaviral infections, and the socio-demographic characteristics and risk factors of infected patients, in kandy, sri lanka. this report discusses the serological evidence of hantavirus infections among 105 suspected leptospirosis patients, 8 of whom had hantavirus antibodies. serotyping elisa showed that these 8 patients had high optical density values for thailand virus. most of the sera showed that ... | 2011 | 21266762 |
| glomerulonephritis emerging shortly after puumala hantavirus infection: a report on 7 patients. | aims: nephropathia epidemica (ne) is mild type of hemorrhagic fever caused by puumala (puu) hantavirus. renal biopsy typically shows acute tubulointerstitial nephritis and complete recovery is the usual outcome. we previously described 5 patients with membranoproliferative glomerulonephritis (mpgn) after acute ne. we now report on 7 more patients who developed biopsy-confirmed glomerulonephritis (gn) during the convalescent phase of ne. material and methods: we present case histories of 7 patien ... | 2011 | 21612760 |
| genetic evidence of hantavirus infections in wild rodents from northwestern colombia. | this report builds on recent serological evidence for the presence of hantavirus in northern colombia by providing sequence-specific and phylogenetic data of hantavirus infections in wild rodents. from august 2007 to august 2008, 354 rodent specimens representing four families were collected in the northwestern antioquia region of colombia. antibodies reactive to sin nombre virus and maciel virus antigens by igg enzyme-linked immunosorbent assay were found in 15 of 109 (14%) cherries cane rats ( ... | 2011 | 21284514 |
| hantavirus-specific iga in saliva and viral antigen in the parotid gland in patients with hemorrhagic fever with renal syndrome. | the hantavirus genus comprises rodent borne, zoonotic viruses of the bunyaviridae family that cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus cardiopulmonary syndrome (hcps) in the americas. rodent saliva contains infectious hantavirus and evidence suggests that hantavirus is also shed in human saliva, but person-to-person transmission is rare. in saliva, immunoglobulin (ig) a is the predominant immunoglobulin class. secretory iga serves as an important first line of ... | 2011 | 21360546 |
| shared ancestry between a newfound mole-borne hantavirus and hantaviruses harbored by cricetid rodents. | discovery of genetically distinct hantaviruses in multiple species of shrews (order soricomorpha, family soricidae) and moles (family talpidae) contests the conventional view that rodents (order rodentia, family muridae and cricetidae) are the principal reservoir hosts and suggests that the evolutionary history of hantaviruses is far more complex than previously hypothesized. we now report on rockport virus (rkpv), a hantavirus identified in archival tissues of the eastern mole (scalopus aquatic ... | 2011 | 21632770 |
| biodiversity and leptospirosis risk: a case of pathogen regulation? | well balanced ecosystems have an essential role in disease regulation, and consequently their correct functioning is increasingly recognised as imperative for maintaining human health. disruptions to ecosystems have been found to increase the risk of several diseases, including hantavirus, lyme disease, ross river virus, malaria and ciguatera fish poisoning. leptospirosis is a globally important emerging zoonosis, caused by spirochaete bacteria, borne by many mammalian hosts, and also transmitte ... | 2011 | 21652151 |
| inactivation of hantaviruses by n-ethylmaleimide preserves virion integrity. | thiol groups of cysteine residues are crucial for the infectivity of various enveloped viruses, but their role in the infectivity of viruses of the family bunyaviridae has thus far not been studied. this report shows that thiol groups are essential to the infectivity of hantaviruses. alkylation of the thiol functional groups using the membrane-permeable compound n-ethylmaleimide (nem) and membrane-impermeable compound 5,5'-dithio-bis-(2-nitrobenzoic acid) (dtnb) showed nem to be a highly effecti ... | 2011 | 21289161 |
| characterization of the interaction between hantavirus nucleocapsid protein (n) and ribosomal protein s19 (rps19). | hantaviruses, members of the bunyaviridae family, are negative-stranded emerging rna viruses and category a pathogens that cause serious illness when transmitted to humans through aerosolized excreta of infected rodent hosts. hantaviruses have evolved a novel translation initiation mechanism, operated by nucleocapsid protein (n), which preferentially facilitates the translation of viral mrnas. n binds to the ribosomal protein s19 (rps19), a structural component of the 40 s ribosomal subunit. in ... | 2011 | 21296889 |
| the cytoplasmic tail of hantavirus gn glycoprotein interacts with rna. | we recently characterized the interaction between the intraviral domains of envelope glycoproteins (gn and gc) and ribonucleoprotein (rnp) of puumala and tula hantaviruses (genus hantavirus, family bunyaviridae). herein we report a direct interaction between spike-forming glycoprotein and nucleic acid. we show that the envelope glycoprotein gn of hantaviruses binds genomic rna through its cytoplasmic tail (ct). the nucleic acid binding of gn-ct is unspecific, as demonstrated by interactions with ... | 2011 | 21807393 |
| a new subtype of thottapalayam virus carried by the asian house shrew (suncus murinus) in china. | recently, many novel hantavirus species carried by insectivore had been found. in order to know whether the insectivore-associated hantaviruses exist in china, asian house shrew (suncus murinus) were analyzed for the presence of hantaviruses. 362 asian house shrews were captured in wenzhou of zhejiang province. hantavirus-specific rna was detected by rt-pcr in lung tissue samples of six shrews. the complete hantavirus s and m segment sequences and also partial l segment sequences were successful ... | 2011 | 21801855 |
| pygmy rice rat as potential host of castelo dos sonhos hantavirus. | to study the dynamics of wild rodent populations and identify potential hosts for hantavirus, we conducted an eco-epidemiologic study in campo novo do parecis, mato grosso state, brazil. we detected and genetically characterized castelo dos sonhos virus found in a species of pygmy rice rat (oligoryzomys utiaritensis). | 2011 | 21801642 |
| high activity of indoleamine 2,3-dioxygenase is associated with renal insufficiency in puumala hantavirus induced nephropathia epidemica. | nephropathia epidemica (ne) is a hemorrhagic fever with renal syndrome caused by puumala hantavirus. the severity of ne varies greatly. the aim of the present study was to evaluate whether serum indoleamine 2,3-dioxygenase (ido) activity is associated with the severity of ne. a prospectively collected cohort of 102 consecutive patients with acute serologically confirmed ne was examined. serum kynurenine, tryptophan, creatinine, crp, and blood cell count were measured for up to 5 consecutive days ... | 2011 | 21328391 |
| hantavirus outbreak in western europe: reservoir host infection dynamics related to human disease patterns. | within europe, puumala virus (puuv) is the causal agent of nephropathia epidemica (ne) in humans, a zoonotic disease with increasing significance in recent years. in a region of belgium with a historically high incidence of ne, bank voles (the puuv reservoir hosts), were monitored for puuv igg antibody prevalence in nine study sites before, during, and after the highest ne outbreak recorded in belgium in 2005. we found that the highest numbers of puuv igg-positive voles coincided with the peak o ... | 2011 | 20450527 |
| prospective study on ocular findings in acute puumala hantavirus infection in hospitalised patients. | to appraise the ocular manifestations of puumala hantavirus evoked haemorrhagic fever with renal syndrome nephropathia epidemica (ne) and to clarify the mechanisms of ocular changes in the largest series of patients examined to date. | 2011 | 20679079 |
| genetic evidence for the presence of two distinct hantaviruses associated with apodemus mice in croatia and analysis of local strains. | in europe, dobrava-belgrade (dobv), saaremaa (saav), and puumala (puuv) viruses are known to cause hemorrhagic fever with renal syndrome (hfrs). all three hantaviruses are now found in croatia. lung tissue samples of 315 apodemus mice trapped in 2003-2004 were screened for the presence of hantaviral n-ag and 20 mice (6.3%) were found either strongly positive or weak/suspected-positive. partial sequences of hantavirus m and s segments were recovered by rt-pcr from six mice and subjected to (phylo ... | 2011 | 21108346 |
| detection of crimean-congo hemorrhagic fever, hanta, and sandfly fever viruses by real-time rt-pcr. | the development of sensitive and specific nucleic acid diagnostic assays for viral pathogens is essential for proper medical intervention. this chapter describes four fluorescence-based pcr assays to detect the crimean-congo hemorrhagic fever (cchfv), andes (andv), hantaan (hanv), and sandfly fever sicilian (sfsv) viruses. these assays are based on species-specific hydrolysis probes targeting the nucleocapsid protein gene for cchfv and sfsv and the glycoprotein gene for andv and hanv. all four a ... | 2011 | 21116810 |
| hantavirus pulmonary syndrome in argentina, 1995-2008. | we report a large case series of hantavirus pulmonary syndrome (hps) in argentina that was confirmed by laboratory results from 1995 through 2008. the geographic and temporal distribution of cases by age, sex, fatality rate, and risk factors for hps was analyzed. a total of 710 cases were unequally distributed among 4 of the 5 argentine regions. different case-fatality rates were observed for each affected region, with a maximum rate of 40.5%. the male-to-female ratio for hps case-patients was 3 ... | 2010 | 21122213 |
| hantaviruses and hantavirus pulmonary syndrome, maranhao, brazil. | to confirm circulation of anajatuba virus in maranhao, brazil, we conducted a serologic survey (immunoglobulin g elisa) and phylogenetic studies (nucleocapsid gene sequences) of hantaviruses from wild rodents and persons with hantavirus pulmonary syndrome. this virus is transmitted by oligoryzomys fornesi rodents and is responsible for hantavirus pulmonary syndrome in this region. | 2010 | 21122229 |
| dobrava-belgrade hantavirus infection mimics acute appendicitis. | dobrava-belgrade hantavirus infection mimicked acute appendicitis in a patient suffering from hemorrhagic fever with renal syndrome in hungary. the 27-year-old man was admitted to the local hospital with severe abdominal pain localized mainly at the right lower quadrant of the abdomen and with fever, nausea, vomiting and bloody diarrhea. based on these findings supported by computerized tomography acute perforated appendicitis was suspected and an explorative laparatomy was performed, which did ... | 2010 | 21075678 |
| tracking hantavirus nucleocapsid protein using intracellular antibodies. | hantavirus nucleocapsid (n) protein is a multifunctional viral macromolecule involved in multiple stages of the viral replication cycle. the intracellular trafficking of n protein during virus assembly remains unclear. | 2010 | 21092325 |
| seroprevalence of hantavirus antibodies among chronic hemodialysis patients in the czech republic. | a total of 348 serum samples were collected from 301 hemodialysis patients with chronic renal failure of four healthcare settings in western bohemia. the sera were screened for the presence of hantavirus antibodies using elisa kits (progen biotechnik gmbh) with hantaan and puumala antigens. specific anti-puumala antibodies were detected in five patients (1.7%). although hantaviruses are known to cause primarily acute renal damage (interstitial nephritis) in eurasia, chronic effects of hantavirus ... | 2010 | 21105569 |
| geographical range of rio mamoré virus (family bunyaviridae, genus hantavirus) in association with the small-eared pygmy rice rat (oligoryzomys microtis). | hantavirus htn.007 was originally isolated from a small-eared pygmy rice rat (oligoryzomys microtis) captured in northeastern peru. the results of analyses of nucleotide and amino acid sequence data in this study indicated that htn.007 is a strain of rio mamoré virus (riomv) which is enzootic in small-eared pygmy rice rat populations in bolivia. as such, the results of this study extend our knowledge of the geographical range of riomv and support the notion that the small-eared pygmy rice rat is ... | 2010 | 20687859 |
| one-step rna pathogen detection with reverse transcriptase activity of a mutated thermostable thermus aquaticus dna polymerase. | we describe the cloning and characterization of a mutated thermostable dna polymerase from thermus aquaticus (taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one-step pcr pathogen detection using established real-time detection methods. we demonstrate that this taq polymerase mutant (taq m1) has similar pcr sensitivity and nuclease activity as the respective taq wild-type dna polymerase. in addition, and in marked contrast to the wild-type, taq m1 e ... | 2010 | 20108275 |
| [dynamics of hantavirus detection in the excretory organs of apodemus mice and its relation to epidemic manifestations of hantavirus infection]. | this paper presents data on the detection of hantavirus in the excretory organs of apodemus mice which are natural carriers of pathogenic hantaviral serotypes in the primorye region. acute infection periods were detected in naturally infected rodents with the highest hantavirus dissemination to the environment in different phases of rodent population cycles. there was a relationship between the rise of morbidity and the seasonal manifestation of hemorrhagic fever with renal syndrome morbidity an ... | 2010 | 20455471 |
| pathology of black creek canal virus infection in juvenile hispid cotton rats (sigmodon hispidus). | the purpose of this study was to assess the effect of inoculum dose on the pathogenesis of black creek canal virus (bccv) infection in the hispid cotton rat (sigmodon hispidus), the principal host of bccv. no sign of illness was observed in any of the 52 juvenile hispid cotton rats inoculated with 3.1, 1.1, -0.9, or -2.9 log(10) median infectious doses(veroe6) (id(50-veroe6)) of bccv and euthanized on day 9, 18, 27, or 54 postinoculation (pi). analysis of virus assay and serological data indicat ... | 2010 | 20455779 |
| hantavirus nucleocapsid protein has distinct m7g cap- and rna-binding sites. | hantaviruses, members of the bunyaviridae family, are emerging category a pathogens that carry three negative stranded rna molecules as their genome. hantavirus nucleocapsid protein (n) is encoded by the smallest s segment genomic rna (viral rna). n specifically binds mrna caps and requires four nucleotides adjacent to the cap for high affinity binding. we show that the n peptide has distinct cap- and rna-binding sites that independently interact with mrna cap and viral genomic rna, respectively ... | 2010 | 20164193 |
| genetic analysis of a hantavirus strain carried by niviventer confucianus in yunnan province, china. | hantavirus genome sequences were recovered from lung tissues of chinese white-bellied rats (niviventer confucianus) captured in yunnan province, china. pairwise comparison of the nucleotide and deduced amino acid sequences of the entire s and partial m and l segments indicated that the newly discovered virus strain, which was designated as strain yn509, was very different from other rodent-borne hantaviruses. phylogenetic analysis showed that the new strain fit into a clade containing da bie sha ... | 2010 | 20600393 |
| development of a lentiviral vector system to study the role of the andes virus glycoproteins. | to infect target cells, enveloped viruses use their virion surface proteins to direct cell attachment and subsequent entry via virus-cell membrane fusion. how hantaviruses enter cells has been largely unexplored. to study early steps of andes virus (andv) cell infection, a lentiviral vector system was developed based on a simian immunodeficiency virus (siv) vector pseudotyped with the andv-gn/gc envelope glycoproteins. the incorporation of gn and gc onto siv-derived vector particles was assessed ... | 2010 | 20619306 |
| sin nombre virus-specific immunoglobulin m and g kinetics in hantavirus pulmonary syndrome and the role played by serologic responses in predicting disease outcome. | sin nombre virus (snv) is the primary cause of hantavirus pulmonary syndrome (hps) in the united states. although other studies have demonstrated a possible association between neutralizing antibody titers and the severity of hps, the exact nature of serologic responses and their association with outcomes have not been fully characterized. | 2010 | 20521946 |
| convalescent pulmonary dysfunction following hantavirus pulmonary syndrome in panama and the united states. | the objective of this study was to document persistent pulmonary symptoms and pulmonary function abnormalities in adults surviving hantavirus pulmonary syndrome (hps). acute infection by most hantaviruses result in mortality rates of 25-35%, while in panama the mortality rate of 10% is contrasted by an unusually high incidence. in all types of hps, the viral prodrome, cardiopulmonary phase due to massive pulmonary capillary leak syndrome, and spontaneous diuresis are followed by a convalescent p ... | 2010 | 20524006 |
| models for the spread and persistence of hantavirus infection in rodents with direct and indirect transmission. | hantavirus, a zoonotic disease carried by wild rodents, is spread among rodents via direct contact and indirectly via infected rodent excreta in the soil. spillover to humans is primarily via the indirect route through inhalation of aerosolized viral particles. rodent-hantavirus models that include direct and indirect transmission and periodically varying demographic and epidemiological parameters are studied in this investigation. two models are analyzed, a nonautonomous system of differential ... | 2010 | 20462286 |
| andes virus disrupts the endothelial cell barrier by induction of vascular endothelial growth factor and downregulation of ve-cadherin. | hantavirus pulmonary syndrome (hps) and hemorrhagic fever with renal syndrome (hfrs) are severe diseases associated with hantavirus infection. high levels of virus replication occur in microvascular endothelial cells but without a virus-induced cytopathic effect. however, virus infection results in microvascular leakage, which is the hallmark of these diseases. ve-cadherin is a major component of adherens junctions, and its interaction with the vascular endothelial growth factor (vegf) receptor, ... | 2010 | 20810734 |
| ecology of hantavirus in a changing world. | hantavirus is a genus of virus represented by 45 different species and is hosted by small mammals, predominantly rats and mice. roughly, half of all hantaviruses cause diseases in humans that vary in morbidity from mild to severe. the natural and anthropogenic changes occurring in the environment appear to be impacting the ecology of hantaviruses and their natural hosts as well as the incidence of hantaviral diseases in humans. although such studies are limited at this time, there is evidence th ... | 2010 | 20536819 |
| central nervous system-related symptoms and findings are common in acute puumala hantavirus infection. | puumala hantavirus (puuv) causes a hemorrhagic fever with renal syndrome (hfrs) also called nephropathia epidemica (ne). recent case reports and retrospective studies suggest that ne may damage the pituitary gland. based on these observations, our goal was to explore the nature of this complication prospectively. | 2010 | 20545485 |
| pathogenic hantaviruses andes virus and hantaan virus induce adherens junction disassembly by directing vascular endothelial cadherin internalization in human endothelial cells. | hantaviruses infect endothelial cells and cause 2 vascular permeability-based diseases. pathogenic hantaviruses enhance the permeability of endothelial cells in response to vascular endothelial growth factor (vegf). however, the mechanism by which hantaviruses hyperpermeabilize endothelial cells has not been defined. the paracellular permeability of endothelial cells is uniquely determined by the homophilic assembly of vascular endothelial cadherin (ve-cadherin) within adherens junctions, which ... | 2010 | 20463083 |
| [hantavirus infection associated to a staphylococcal sepsis. report of one case]. | we report a previously healthy 36-year-old mole who developed a pulmonary syndrome caused by hantavirus infection. he was admitted to an intensive care unit in shock and respiratory failure and died four hours after admission. blood cultures were positive for staphylococcus aureus. this patient could be an index case that shows, for the first time, an association of hantavirus pulmonary syndrome with staphylococcus aureus infection similar to what happens with other viruses. | 2010 | 20556338 |
| interaction of hantavirus nucleocapsid protein with ribosomal protein s19. | hantaviruses, members of the bunyaviridae family, are emerging category a pathogens that initiate the translation of their capped mrnas by a novel mechanism mediated by viral nucleocapsid protein (n). n specifically binds to the mrna 5' m7g cap and 40s ribosomal subunit, a complex of 18s rrna and multiple ribosomal proteins. here, we show that n specifically interacts with the ribosomal protein s19 (rps19), located at the head region of the 40s subunit. we suggest that this n-rps19 interaction f ... | 2010 | 20844026 |
| antagonism of type i interferon responses by new world hantaviruses. | evasion of interferon (ifn)-mediated antiviral signaling is a common defense strategy for pathogenic rna viruses. to date, research on ifn antagonism by hantaviruses is limited and has focused on only a subset of the numerous recognized hantavirus species. the host ifn response has two phases, an initiation phase, resulting in the induction of alpha/beta ifn (ifn-α/β), and an amplification phase, whereby ifn-α/β signals through the jak/stat pathway, resulting in the establishment of the cellular ... | 2010 | 20844031 |
| interaction between hantaviral nucleocapsid protein and the cytoplasmic tail of surface glycoprotein gn. | hantaviral n and gn proteins were shown to interact, thus providing the long-awaited evidence for one of the crucial steps in the virus replication at which rnps are directed to the site of the virus assembly. using pull-down assay and point mutagenesis it was demonstrated that intact, properly folded zinc fingers in the gn protein cytoplasmic tail as well as the middle domain of the n protein (that includes aa residues 80-248) are essential for the interaction. | 2010 | 20566401 |
| extinction of refugia of hantavirus infection in a spatially heterogeneous environment. | we predict an abrupt observable transition, on the basis of numerical studies, of hantavirus infection in terrain characterized by spatially dependent environmental resources. the underlying framework of the analysis is that of fisher equations with an internal degree of freedom, the state of infection. the unexpected prediction is of the sudden disappearance of refugia of infection in spite of the existence of supercritical (favorable) food resources, brought about by reduction of their spatial ... | 2010 | 20866661 |
| new world hantaviruses activate ifnlambda production in type i ifn-deficient vero e6 cells. | hantaviruses indigenous to the new world are the etiologic agents of hantavirus cardiopulmonary syndrome (hcps). these viruses induce a strong interferon-stimulated gene (isg) response in human endothelial cells. african green monkey-derived vero e6 cells are used to propagate hantaviruses as well as many other viruses. the utility of the vero e6 cell line for virus production is thought to owe to their lack of genes encoding type i interferons (ifn), rendering them unable to mount an efficient ... | 2010 | 20567522 |
| hantavirus infections in fluctuating host populations: the role of maternal antibodies. | infected females may transfer maternal antibodies (matabs) to their offspring, which may then be transiently protected against infections the mother has encountered. however, the role of maternal protection in infectious disease dynamics in wildlife has largely been neglected. here, we investigate the effects of puumala hantavirus (puuv)-specific matabs on puuv dynamics, using 7 years' data from a cyclic bank vole population in finland. for the first time to our knowledge, we partition seroposit ... | 2010 | 20591866 |
| enhanced thrombin formation and fibrinolysis during acute puumala hantavirus infection. | nephropathia epidemica (ne) is a viral hemorrhagic fever with renal syndrome associated with thrombocytopenia and mild bleeding. we assessed activation of coagulation and fibrinolysis during the acute phase of ne. | 2010 | 20579693 |
| [hantavirus]. | 2010 | 20942073 | |
| temporal and spatial host abundance and prevalence of andes hantavirus in southern argentina. | andes virus (and) is a hantavirus hosted by the sigmodontine rodent oligoryzomys longicaudatus in southern argentina, where it is responsible for most cases of hantavirus pulmonary syndrome (hps). our study provides data about the spatial variation in abundance of the rodent host of and hantavirus. we report results of a longitudinal study performed in a locality of the andean region of chubut province. from november 2003 (spring) to july 2006 (winter), o. longicaudatus was the most common speci ... | 2010 | 20645121 |
| the relative abundance of deer mice with antibody to sin nombre virus corresponds to the occurrence of hantavirus pulmonary syndrome in nearby humans. | abstract sin nombre virus (snv) is the principal cause of hantavirus pulmonary syndrome (hps) in the united states and deer mice (peromyscus maniculatus) are its principal rodent host, and thus the natural cycle of the virus is related to the occurrence of hps. prevalence of rodent infection appears to be associated with fluctuations in deer mouse populations and, indirectly, with timing and amount of precipitation, a complex of biologic events. given that rodent population abundances fluctuate, ... | 2010 | 20954865 |
| in vivo kinetics and biodistribution of a hantaan virus dna vaccine after intramuscular injection in mice. | to study the kinetics in vivo of a hantaan virus dna vaccine, we constructed a fusion dna vaccine, pegfp/s, by cloning the s segment of hantavirus into the vector, pegfp-c1, which encodes green fluorescent protein egfp. in this report, we provide evidence that pegfp/s was distributed and persistently expressed for more than 60 days in several organs after inoculation. our findings suggest that the persistent immune responses induced by a hantaan virus dna vaccine are likely due to the plasmid pe ... | 2010 | 20960291 |
| satellite derived forest phenology and its relation with nephropathia epidemica in belgium. | the connection between nephropathia epidemica (ne) and vegetation dynamics has been emphasized in recent studies. changing climate has been suggested as a triggering factor of recently observed epidemiologic peaks in reported ne cases. we have investigated whether there is a connection between the ne occurrence pattern in belgium and specific trends in remotely sensed phenology parameters of broad-leaved forests. the analysis of time series of the modis enhanced vegetation index revealed that ch ... | 2010 | 20644685 |
| pathogenic landscapes: interactions between land, people, disease vectors, and their animal hosts. | landscape attributes influence spatial variations in disease risk or incidence. we present a review of the key findings from eight case studies that we conducted in europe and west africa on the impact of land changes on emerging or re-emerging vector-borne diseases and/or zoonoses. the case studies concern west nile virus transmission in senegal, tick-borne encephalitis incidence in latvia, sandfly abundance in the french pyrenees, rift valley fever in the ferlo (senegal), west nile fever and t ... | 2010 | 20979609 |
| hantavirus infection: a neglected diagnosis in thrombocytopenia and fever? | thrombocytopenia, fever, and acute renal failure are characteristic features of nephropathia epidemica, the predominant hantavirus infection in europe. however, clinical presentation and blood cell counts may point to other disorders, such as a hematologic disease, particularly when impairment of renal function is not evident. this differential diagnosis often results in further extensive and unnecessary testing. we describe 3 patients with hantavirus infection with no renal failure, in whom a h ... | 2010 | 21037045 |
| the 3' untranslated region of the andes hantavirus small mrna functionally replaces the poly(a) tail and stimulates cap-dependent translation initiation from the viral mrna. | in the process of translation of eukaryotic mrnas, the 5' cap and the 3' poly(a) tail interact synergistically to stimulate protein synthesis. unlike its cellular counterparts, the small mrna (smrna) of andes hantavirus (andv), a member of the bunyaviridae, lacks a 3' poly(a) tail. here we report that the 3' untranslated region (3'utr) of the andv smrna functionally replaces a poly(a) tail and synergistically stimulates cap-dependent translation initiation from the viral mrna. stimulation of tra ... | 2010 | 20660206 |
| [hemorrhagic fever with renal syndrome during pregnancy: case report]. | hemorrhagic fever with renal syndrome (hfwrs) is an acute infectious disease with abrupt onset, high fever, renal failure and frequent hemorragies. hfwrs during the pregnancy has relatively low occurrence, with only few described cases mostly in the region of the former soviet union. although, according to the data from the literature, the disease is less severe during the pregnancy, the eclampsy, stillbirth and fetal infection were also described. | 2010 | 21053473 |
| [construction and immunogenic study of recombinant adenovirus containing chimeric gene g2s0.7 and ctl epitopes of hantaan virus]. | to express g2 fragment of m segment and 0.7 kb fragment of s segment and several ctl epitopes of s segment in adenovirus expression system and investigate the immunological properties of hantaan virus chimeric gene. | 2010 | 21055343 |
| hantavirus infections in humans and animals, china. | hemorrhagic fever with renal syndrome (hfrs) is a serious public health problem in the people's republic of china. although 7 sero/genotypes of hantaviruses have been found in rodents, only hantaan virus (carried by apodemus agrarius mice) and seoul virus (carried by rattus norvegicus rats) reportedly cause disease in humans. during 1950-2007, a total of 1,557,622 cases of hfrs in humans and 46,427 deaths (3%) were reported in china. hfrs has been reported in 29 of 31 provinces in china. after i ... | 2010 | 20678311 |
| infection of in vivo differentiated human mast cells with hantaviruses. | increased vascular permeability is a key feature of the pathological symptoms caused by hantaviruses. here, we analysed the interaction between hantaviruses and mast cells, which regulate vascular homeostasis. in highly purified human skin mast cells increasing amounts of hantaan (htnv) and, to a lower extent, prospect hill (phv) virions were produced. replication was confirmed by the production of viral plus-strand rna as determined by a virus strand-specific rt-pcr. phv but not htnv elicited e ... | 2010 | 20071485 |
| comparison of the effects of puumala and dobrava viruses on early and long-term renal outcomes in patients with haemorrhagic fever with renal syndrome. | the clinical course and outcome of patients with haemorrhagic fever with renal syndrome (hfrs) caused by puumala (puuv) and dobrava viruses (dobv) were analyzed and whether it left long-term consequences on kidney function after 10 years was evaluated. | 2010 | 20470304 |
| the severity of puumala hantavirus induced nephropathia epidemica can be better evaluated using plasma interleukin-6 than c-reactive protein determinations. | nephropathia epidemica (ne) is a scandinavian type of hemorrhagic fever with renal syndrome caused by puumala hantavirus. the clinical course of the disease varies greatly in severity. the aim of the present study was to evaluate whether plasma c-reactive protein (crp) and interleukin (il)-6 levels associate with the severity of ne. | 2010 | 20500875 |
| the severity of acute puumala hantavirus infection does not predict the long-term outcome of patients. | we have found greater urinary protein excretion and higher glomerular filtration rate (gfr) and blood pressure in patients 6 years after acute nephropathia epidemica (ne) compared with seronegative controls. the present aim was to establish whether the long-term outcome is determined by the severity of acute illness. | 2010 | 20502044 |
| steep rise in notified hantavirus infections in germany, april 2010. | from january to april 2010, 396 hantavirus infections were notified in germany, a considerable increase compared with previous years (mean: 83 for january-april 2004-2009) including the record-setting year, 2007 (n=232 january-april). most patients are residents of known puumala virus endemic areas in southern germany. the recent increase in notified hantavirus infections is probably due to an increased population density of the main animal reservoir, the bank vole (myodes glareolus). | 2010 | 20504391 |
| generation and characterization of genetic reassortants between puumala and prospect hill hantavirus in vitro. | hantaviruses belong to the family bunyaviridae characterized by tri-segmented rna genomes. depending on the hantavirus species, infection can lead to hantavirus cardiopulmonary or haemorrhagic fever with renal syndrome. in vitro studies suggest that pathogenic hantaviruses evade induction of innate antiviral responses, and this ability might determine the virulence in humans. since reverse genetic systems are not available, in vitro reassortment is currently the only way to culture defined hanta ... | 2010 | 20505009 |
| relationship of human behavior within outbuildings to potential exposure to sin nombre virus in western montana. | sin nombre virus (snv) causes hantavirus cardiopulmonary syndrome (hcps) in humans. transmission of snv among the deer mouse (peromyscus maniculatus) host predominates during spring and summer, and is greater in peridomestic than sylvan settings where, protected from uv light, snv may survive longer. incidence of hcps reflects these times and settings and is associated with inhalation of mouse excreta. little is known, however, about how human use of outbuildings contributes to potential exposur ... | 2010 | 20508970 |
| hantaviruses. | hantaviruses affect people worldwide, yet they remain poorly understood. this article explores the known history of hantaviruses. it describes diagnostic methods and potential options for treatment and prevention. | 2010 | 20513542 |
| models for the spread and persistence of hantavirus infection in rodents with direct and indirect transmission. | hantavirus, a zoonotic disease carried by wild rodents, is spread among rodents via direct contact and indirectly via infected rodent excreta in the soil. spillover to humans is primarily via the indirect route through inhalation of aerosolized viral particles. rodent-hantavirus models that include direct and indirect transmission and periodically varying demographic and epidemiological parameters are studied in this investigation. two models are analyzed, a nonautonomous system of differential ... | 2010 | 20104956 |
| [hantavirosis: clinical and epidemiological characteristics of pediatric patients in chile]. | the first cases of hantavirus cardiopulmonary syndrome in children were described in the united states and represented 8% of the total reported cases; in chile, the proportion of pediatric cases represents 15% of all national cases. | 2010 | 20140316 |
| seoul virus suppresses nf-kappab-mediated inflammatory responses of antigen presenting cells from norway rats. | hantavirus infection reduces antiviral defenses, increases regulatory responses, and causes persistent infection in rodent hosts. to address whether hantaviruses alter the maturation and functional activity of antigen presenting cells (apcs), rat bone marrow-derived dendritic cells (bmdcs) and macrophages (bmdms) were generated and infected with seoul virus (seov) or stimulated with tlr ligands. seov infected both dcs and macrophages, but copies of viral rna, viral antigen, and infectious virus ... | 2010 | 20170933 |
| hantavirus pulmonary syndrome. | hantavirus pulmonary syndrome, also known as hantavirus cardiopulmonary syndrome, is a recently described infectious syndrome found throughout the americas. although infection is sporadic and uncommon compared with other atypical pneumonia syndromes, its high mortality rate warrants the maintenance of a high index of suspicion in rural settings. because no specific therapies are available for the disease, prevention and early recognition play an important role in reducing mortality from the dise ... | 2010 | 20171551 |
| pathogenic hantaviruses direct the adherence of quiescent platelets to infected endothelial cells. | hantavirus infections are noted for their ability to infect endothelial cells, cause acute thrombocytopenia, and trigger 2 vascular-permeability-based diseases. however, hantavirus infections are not lytic, and the mechanisms by which hantaviruses cause capillary permeability and thrombocytopenia are only partially understood. the role of beta(3) integrins in hemostasis and the inactivation of beta(3) integrin receptors by pathogenic hantaviruses suggest the involvement of hantaviruses in altere ... | 2010 | 20181715 |