Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| transaminase on rotavirus gastroenteritis. | background: children infected with rotavirus often show increased levels of transaminase, and their symptom is characterized by white stool similar as in biliary atresia. rotavirus infections are also sporadically accompanied with convulsions, encephalopathy and reye syndrome. methods: we evaluated the results of the liver function tests, mainly the elevation of transaminase and interleukin (il)-6 in rotavirus gastroenteritis with or without convulsions in order to better understand their clin ... | 2011 | 22136601 |
| Phages bearing affinity peptides to bovine rotavirus differentiate the virus from other viruses. | The aim of this study was to identify potential ligands and develop a novel diagnostic test to pathogenic bovine rotavirus (BRV) using phage display technology. The viruses were used as an immobilized target followed by incubation with a 12-mer phage display random peptide library. After five rounds of biopanning, phages had a specific binding activity to BRV were isolated. DNA sequencing indicated that phage displayed peptides HVHPPLRPHSDK, HATNHLPTPHNR or YPTHHAHTTPVR were potential ligands to ... | 2011 | 22163050 |
| Accelerating introduction of new vaccines: barriers to introduction and lessons learned from the recent Haemophilus influenzae type B vaccine experience. | Adoption of new vaccines in developing countries is critical to reducing child mortality and meeting Millennium Development Goal 4. However, such introduction has historically suffered from significant delays that can be attributed to various factors including (i) lack of recognition of the value of a vaccine, (ii) factors related to weak health systems, and (iii) policy considerations. Recently, the Global Alliance for Vaccines and Immunization (GAVI) supported efforts to accelerate the introdu ... | 2011 | 21893547 |
| [Current events in vaccination]. | The annual meeting of the Infectious Disease Society of America (IDSA) ; which brought together nearly 5000 participants from over 80 countries in Vancouver, Canada, October 21 to 24, 2010 ; provided a review of the influenza (H1N1) 2009 pandemic, evaluated vaccination programmes and presented new vaccines under development. With 12,500 deaths in the United States in 2009-2010, the influenza (H1N1) 2009 pandemic was actually less deadly than the seasonal flu. But it essentially hit the young, an ... | 2011 | 22019286 |
| Healthy-days time equivalents for outcomes of acute rotavirus infections. | Rotavirus is the most common cause of severe gastroenteritis in infants and young children worldwide. Health-state utility measures used in economic evaluations of rotavirus vaccines do not reflect differences between mild and severe symptoms of rotavirus gastroenteritis and, therefore, do not adequately capture preferences for non-fatal outcomes associated with rotavirus common in industrialized countries. This paper describes the development and results of a survey specifically designed to dev ... | 2011 | 21864612 |
| unexpected benefits of rotavirus vaccination in the united states. | 2011 | 21878426 | |
| Tilt-pair analysis of images from a range of different specimens in single-particle electron cryomicroscopy. | The comparison of a pair of electron microscope images recorded at different specimen tilt angles provides a powerful approach for evaluating the quality of images, image-processing procedures, or three-dimensional structures. Here, we analyze tilt-pair images recorded from a range of specimens with different symmetries and molecular masses and show how the analysis can produce valuable information not easily obtained otherwise. We show that the accuracy of orientation determination of individua ... | 2011 | 21939668 |
| bluetongue virus vp1 polymerase activity in vitro: template dependency, dinucleotide priming and cap dependency. | bluetongue virus (btv) protein, vp1, is known to possess an intrinsic polymerase function, unlike rotavirus vp1, which requires the capsid protein vp2 for its catalytic activity. however, compared with the polymerases of other members of the reoviridae family, btv vp1 has not been characterized in detail. | 2011 | 22110731 |
| Low prevalence of rotavirus and high prevalence of norovirus in hospital and community wastewater after introduction of rotavirus vaccine in nicaragua. | Rotavirus (RV) and norovirus (NoV) are major causes of pediatric diarrhea and are altogether associated with approximately 800,000 deaths in young children every year. In Nicaragua, national RV vaccination program using the pentavalent RV5 vaccine from Merck was implemented in October 2006. To determine whether RV vaccination decreased the overall number of RV infections, we investigated the occurrence of RV and NoV in wastewater in the city of León from July 2007 to July 2008 and compared these ... | 2011 | 22016794 |
| maintaining vaccine delivery following the introduction of the rotavirus and pneumococcal vaccines in thailand. | although the substantial burdens of rotavirus and pneumococcal disease have motivated many countries to consider introducing the rotavirus vaccine (rv) and heptavalent pneumococcal conjugate vaccine (pcv-7) to their national immunization programs (epis), these new vaccines could affect the countries' vaccine supply chains (i.e., the series of steps required to get a vaccine from their manufacturers to patients). we developed detailed computational models of the trang province, thailand, vaccine ... | 2011 | 21931805 |
| interaction of enterovirus infection and cow's milk-based formula nutrition in type 1 diabetes-associated autoimmunity. | background: enteral virus infections and early introduction of cow's milk (cm)-based formula are among the suggested triggers of type 1 diabetes (t1d)- associated autoimmunity, although studies on their role have remained contradictory. here, we aimed to analyse whether interactions between these factors might clarify the controversies. materials: the study population comprised 107 subjects developing positivity for at least two t1d-associated autoantibodies and 446 control subjects from the fin ... | 2011 | 21922634 |
| Global study of viral diarrhea in hospitalized children in Spain: results of structural surveillance of viral gastroenteritis net work (VIGESS-net) 2006-2008. | Detection and characterization of gastroenteritis cases (viruses) was conducted during eleven years through the surveillance VIGESS-net, which was created in an effort to conduct a structured surveillance of rotavirus genotypes co-circulating in Spain. | 2011 | 21963269 |
| intussusception after rotavirus vaccination--spontaneous reports. | 2011 | 22129263 | |
| identification of lineage iii of g12 rotavirus strains in diarrheic children in the northern region of brazil between 2008 and 2010. | this study reports on the surveillance for rotavirus genotypes and the identification of g12 human rotavirus in the northern region of brazil. rotavirus-positive samples were collected from children <5 years of age with acute diarrhea from january 2008 to october 2010. g2p[4] was the most prevalent genotype, accounting for 45.6% (126/303) of cases. five rotavirus strains bearing g12p[6] genotype specificity were detected. phylogenetic analysis of the vp7 gene showed that g12 strains clustered in ... | 2011 | 21947565 |
| [rotavirus vaccines: a long and difficult journey]. | diarrhea is the second cause of death worldwide. the main causative agent in infants and children less than 5 years is rotavirus. consequently, for the world health organization and the global alliance for vaccines and immunization (gavi), rotavirus vaccination is an urgent priority. the global distribution of rotavirus serotypes varies from country to country, but in 80-90% of cases is caused by five types: g1p[8], g2p[4], g3p[8], g4p[8] and g9p[8]. currently, two rotavirus vaccines are availab ... | 2011 | 22112634 |
| glycan-modifying bacteria-derived soluble factors from bacteroides thetaiotaomicron and lactobacillus casei inhibit rotavirus infection in human intestinal cells. | rotaviruses attach to intestinal cells in a process that requires glycan recognition. some bacteria from the gut microflora have been shown to modify cell-surface glycans. in this study, human intestinal cultured cells were incubated with bacteria-derived soluble factors and infected with rotavirus. results show that only bacterial soluble factors that increase cell-surface galactose namely, those of bacteroides thetaiotaomicron and lactobacillus casei were able to efficiently block rotavirus in ... | 2011 | 22079149 |
| [hemicerebellitis associated with rotavirus gastroenteritis.] | 2011 | 22206883 | |
| patient volume and quality of care for young children hospitalized with acute gastroenteritis. | to explore the relationship between the volume of children admitted to the hospital with acute gastroenteritis and adherence to recommended quality indicators. | 2011 | 21893651 |
| vaccines and future global health needs. | increased international support for both research into new vaccines and their deployment in developing countries has been evident over the past decade. in particular, the gavi alliance has had a major impact in increasing uptake of the six common infant vaccines as well as those against hepatitis b and yellow fever. it further aims to introduce pneumococcal and rotavirus vaccines in the near future and several others, including those against human papillomavirus, meningococcal disease, rubella a ... | 2011 | 21893548 |
| 2008 estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis. | background: who recommends routine use of rotavirus vaccines in all countries, particularly in those with high mortality attributable to diarrhoeal diseases. to establish the burden of life-threatening rotavirus disease before the introduction of a rotavirus vaccine, we aimed to update the estimated number of deaths worldwide in children younger than 5 years due to diarrhoea attributable to rotavirus infection. methods: we used pubmed to identify studies of at least 100 children younger than 5 y ... | 2011 | 22030330 |
| childhood diarrhea deaths after rotavirus vaccination in mexico. | 2011 | 21864191 | |
| occurrence of norovirus and other enteric viruses in untreated groundwaters of korea. | a total of 39 water samples from 23 different groundwater wells in korea were collected and analyzed in order to monitor the occurrence of norovirus (nov) and other indicator microbes as the first part of a national survey of groundwater. more than 500 l of untreated groundwater were filtered through 1mds filters. following elution and concentration by organic flocculation, pcr and sequence analysis were employed to detect and identify nov, enterovirus, rotavirus, hepatitis a virus and adenoviru ... | 2011 | 21976201 |
| incidence of intussusception among infants in a large commercially insured population in the united states. | background: to estimate the incidence of intussusception among infants treated in inpatient and emergency department settings during the period preceding the us launch of second generation rotavirus vaccines. methods: from a large us health insurance claims database, we sampled 100,000 infants aged 1 to 3 months at first diphtheria-tetanus-acellular pertussis (dtap) vaccination between 2001 and 2005. potential intussusception cases were identified on the basis of claims and confirmed by medical ... | 2011 | 22173141 |
| immunogenicity, reactogenicity and safety of the 10-valent pneumococcal nontypeable haemophilus influenzae protein d conjugate vaccine (phid-cv) in mexican infants. | the immunogenicity and safety of the 10-valent pneumococcal conjugate vaccine, phid-cv, have been documented in european and asian studies. in this open study conducted in mexico (nct00489554), 230 healthy infants received three doses of phid-cv and dtpa-hbv-ipv/hib vaccines at 2, 4 and 6 months of age and two doses of oral human rotavirus vaccine at 2 and 4 months. serotype-specific pneumococcal responses and opsonophagocytic activity (opa) were measured one month post-dose 3. phid-cv's primary ... | 2011 | 22048109 |
| impact of rotavirus vaccination on childhood gastroenteritis-related mortality and hospital discharges in panama. | background: rotavirus vaccination was introduced in panama in march 2006. this study was carried out in order to describe the trends in gastroenteritis-related (ger) hospitalizations and mortality in children <5 years of age during the pre- and post-vaccination periods. methods: data from the expanded program on immunization (ministry of health) were used to calculate vaccine coverage. ger mortality and hospitalizations were obtained through database review of the contraloría general de la repúb ... | 2011 | 22154592 |
| gavi rolls out vaccines against child killers to more countries. | 2011 | 21957215 | |
| health economics of rotavirus immunization in vietnam: potentials for favorable cost-effectiveness in developing countries. | introduction: rotavirus is the most common cause of severe diarrhoea worldwide. vietnam is situated in the region of high rotavirus infection incidence and eligible for financial support to introduce rotavirus vaccines into the expanded program of immunization (epi) from the gavi. this study was designed to assess the cost-effectiveness of rotavirus immunization in vietnam, explicitly the use of rotateq(®) and to assess the affordability of implementing universal rotavirus immunization based on ... | 2011 | 22119590 |
| antigenemia and cytokine expression in rotavirus gastroenteritis in children. | background: antigenemia is commonly found in children with rotavirus infection, although its clinical significance is undetermined. the aim of this study was to evaluate the association of antigenemia with clinical manifestations and cytokine profiles in children infected by rotavirus. methods: in total, 68 children hospitalized with rotavirus gastroenteritis were enrolled. serum samples were collected for detection of antigenemia and viremia. clinical, laboratory and demographic data were analy ... | 2011 | 22169122 |
| a multi-center, qualitative assessment of pediatrician and maternal perspectives on rotavirus vaccines and the detection of porcine circovirus. | in 2010, researchers using novel laboratory techniques found that us-licensed rotavirus vaccines contain dna or dna fragments from porcine circovirus (pcv), a virus common among pigs but not believed to cause illness in humans. we sought to understand pediatricians' and mothers' perspectives on this finding. | 2011 | 21943237 |
| rotavirus shedding in premature infants following first immunization. | there is limited data regarding rotavirus vaccine shedding in premature infants. we describe the natural history of rotavirus shedding in premature infants in the 2-week period following first immunization with rotateq(®), the pentavalent rotavirus vaccine (rv5), and the risk for symptomatic transmission to household contacts (hhc). | 2011 | 21856359 |
| Effect of Detection Methods on Risk Estimates of Exposure to Biosolids-Associated Human Enteric Viruses. | This study illustrates the effect of virus detection methods on estimates of risks of infection of biosolids-associated viruses for occupational workers and residential population during a hypothetical exposure of biosolids. Five gastroenteritis-associated human enteric viruses-enteroviruses (echovirus-12, enteroviruse types 68-71), adenoviruses, rotaviruses, and noroviruses genotype-I-were considered to represent human enteric viruses for risk estimation purposes. Ingested viral doses were calc ... | 2011 | 22050302 |
| sensitivity of poliovirus type 1 chat strain and rotavirus sa-11 to e-beam irradiation on iceberg lettuce and spinach: quantifying the reduction in potential health risks. | fresh produce such as lettuce and spinach serve as routes of foodborne illnesses. the u.s. fda has approved the use of ionizing irradiation upto 4 kgy as a pathogen kill step for fresh cut lettuce and spinach. the focus of this study was to determine the inactivation of poliovirus and rotavirus on lettuce and spinach when exposed to varying doses of high energy e-beam irradiation and to calculate the theoretical reduction in infection risks that can be achieved under different contamination scen ... | 2011 | 22179244 |
| co-circulation of g1, g2 and g9 rotaviruses in hospitalized patients in bangladesh during 2006-2009. | rotavirus strain diversity in bangladesh has been explored since 1985 and as seen in other parts of the world, rotaviruses have shown tremendous strain diversity overtime. rotavirus antigen was detected in stool specimens using a solid-phase sandwich-type enzyme immunoassay. a multiplex reverse transcription polymerase chain reaction (rt-pcr) was performed for rotavirus g and p genotypes. this current study was carried out between 2006 and 2009 during which time 1,607 (23%) of 7,058 fecal specim ... | 2011 | 21860255 |
| detection of avian rotaviruses of groups a, d, f and g in diseased chickens and turkeys from europe and bangladesh. | avian rotaviruses (avrvs) represent a diverse group of intestinal viruses, which are suspected as the cause of several diseases in poultry with symptoms of diarrhoea, growth retardation or runting and stunting syndrome (rss). to assess the distribution of avrvs in chickens and turkeys, we have developed specific pcr protocols. these protocols were applied in two field studies investigating faecal samples or intestinal contents of diseased birds derived from several european countries and banglad ... | 2011 | 22079218 |
| vaccine-preventable diseases and their impact on latin american children. | a joint meeting of the latin american society of pediatric infectious diseases, the dominican society of pediatrics and the dominican society of vaccinology was held in the dominican republic. this report highlights the most relevant issues that were presented and discussed about vaccine-preventable diseases, their epidemiology and impact in latin american children, the need to move forward and expand national immunization programs and the economical and political obstacles to introduce ?new? va ... | 2011 | 22085169 |
| inhibitory effects of bile acids and synthetic farnesoid x receptor agonists on rotavirus replication. | rotaviruses (group a rotaviruses) are the most important cause of severe gastroenteritis in infants and children worldwide. currently, an antiviral drug is not available and information on therapeutic targets for antiviral development is limited for rotavirus infection. previously, it was shown that lipid homeostasis is important in rotavirus replication. since farnesoid x receptor (fxr) and its natural ligands bile acids (such as chenodeoxycholic acid [cdca]) play major roles in cholesterol and ... | 2011 | 21957312 |
| horizontal transmission of a human rotavirus vaccine strain--a randomized, placebo-controlled study in twins. | transmission of excreted vaccine-derived infectious virus from vaccinated to unvaccinated individuals is possible within close contacts. this randomized (1:1), double-blind study evaluated the potential for transmission of human rotavirus vaccine strain, hrv (rotarix™) from vaccine recipients to unvaccinated close contacts (twins). 100 pairs of healthy twins aged 6-14 weeks at the time of dose 1 of hrv vaccine/placebo were enrolled and one randomly selected twin from each pair received two vacci ... | 2011 | 22008819 |
| national and state vaccination coverage among children aged 19-35 months--united states, 2010. | the national immunization survey (nis) monitors vaccination coverage among children aged 19-35 months using a random-digit-dialed sample of telephone numbers of households to evaluate childhood immunization programs in the united states. this report describes the 2010 nis coverage estimates for children born during january 2007-july 2009. nationally, vaccination coverage increased in 2010 compared with 2009 for ≥ 1 dose of measles, mumps, and rubella vaccine (mmr), from 90.0% to 91.5%; ≥ 4 doses ... | 2011 | 21881546 |
| rotavirus nonstructural protein 1 antagonizes innate immune response by interacting with retinoic acid inducible gene i. | abstract: | 2011 | 22152002 |
| monitoring impact and effectiveness of rotavirus vaccination. | rotavirus infection is the most common cause of severe gastroenteritis in children <5 years of age globally. since 2009, the who has recommended inclusion of rotavirus vaccine in the national immunization programs of all countries. data regarding rotavirus vaccine impact and effectiveness under conditions of routine use are important for encouraging countries to implement vaccination programs. in the absence of a national rotavirus vaccination program in france, the ivanhoe study was initiated t ... | 2011 | 21854307 |
| rotavirus infection induces the unfolded protein response of the cell and controls it through the nonstructural protein nsp3. | the unfolded protein response (upr) is a cellular mechanism that is triggered in order to cope with the stress caused by the accumulation of misfolded proteins in the endoplasmic reticulum (er). this response is initiated by the endoribonuclease inositol-requiring enzyme 1 (ire1), activating transcription factor 6 (atf6), and pkr-like er kinase, which increase the expression of the genes involved in the folding and degradation processes and decrease the protein input into the er by inhibiting tr ... | 2011 | 21937647 |
| antigenemia, rnaemia, and innate immunity in children with acute rotavirus diarrhea. | antigenemia is commonly detected in children with acute rotavirus diarrhea, but the prevalence of viremia has not been clearly defined. we examined antigenemia in plasma and rnaemia in peripheral blood mononuclear cells (pbmc) of children with acute diarrhea by eia, rt-pcr, and southern hybridization, using primers and a probe specific to rotavirus nsp4 gene. we detected the presence of rotavirus antigen in 33.3% and almost full-length nsp4 gene in 70.8% of the acute-phase plasma and pbmc, respe ... | 2011 | 22176605 |
| compatible immuno-nasba loc device for quantitative detection of waterborne pathogens: design and validation. | waterborne pathogens usually pose a global threat to animals and human beings. there has been a growing demand for convenient and sensitive tools to detect the potential emerging pathogens in water. in this study, a lab-on-a-chip (loc) device based on the real-time immuno-nasba (immuno-nucleic acid sequence-based amplification) assay was designed, fabricated and verified. the disposable immuno-nasba chip is modelled on a 96-well elisa microplate, which contains 43 reaction chambers inside the bi ... | 2011 | 22146918 |
| Increased prevalence of rotavirus among children associated gastroenteritis in Riyadh Saudi Arabia. | ABSTRACT: The aim of this study is to assess the epidemiology along with the molecular structure of rotavirus causing pediatric diarrhea among Saudi patients. However, in this report we sited the epidemiological reflect coming from our project. METHODS: One thousand and seven diarrheal stool samples had been collected between Jan1st, 2008 and OCT 31st, 2010 from hospitalized patients below the age of 5 year. Samples were then examined using Enzyme-linked immunosorbent assay (ELISA). Demographic ... | 2011 | 22176997 |
| outstanding challenges for rotavirus vaccine introduction in low-income countries--a systematic review. | rotavirus infections are the most common cause of severe diarrhoea in children worldwide. two internationally licensed rotavirus vaccines have proven to be efficacious in middle and high-income countries and they could potentially be valuable tools for the prevention of rotavirus-associated diarrhoea in low-income countries where the disease burden is greatest. however, before the vaccines can be introduced into the national immunisation programmes in these countries, many challenges related to ... | 2011 | 21975154 |
| developing novel antisecretory drugs to treat infectious diarrhea. | diarrhea, a disease of poverty and poor sanitation, kills an estimated two million children each year. oral rehydration therapy is a very simple and inexpensive treatment that has significantly reduced mortality from secretory diarrhea caused by rotavirus, cholera and enterotoxigenic escherichia coli. the efficacy and adoption of oral rehydration therapy would be enhanced by a drug that reduces fluid loss associated with these diseases and alleviates disease symptoms. secretion and absorption by ... | 2011 | 21859305 |
| molecular detection of three gastroenteritis viruses in urban surface waters in beijing and correlation with levels of fecal indicator bacteria. | to assess the presence of three gastroenteritis viruses responsible for human acute gastroenteritis in surface water, a 1-year study was carried out in the city of beijing, china. a total of 108 urban surface water samples were collected from nine collection sites which were defined with a global positioning system in rivers or lakes from september 2006 to august 2007. the water samples were subjected to virus concentration using an ha electronegative filter, followed by polymerase chain reactio ... | 2011 | 21915594 |
| analysis of rotavirus antigenemia in hematopoietic stem cell transplant recipients. | systemic rotavirus infection, such as rotavirus antigenemia, has been found in immunocompetent rotavirus gastroenteritis patients. however, the pathogenesis of rotavirus infection in immunocompromised transplant recipients remains unclear. enzyme-linked immunosorbent assay was used to measure rotavirus antigen levels in serially collected serum samples obtained from 62 pediatric patients receiving allogeneic hematopoietic stem cell transplants (hsct). rotavirus antigen was detected in 43 (6.8%) ... | 2011 | 22093089 |
| probiotics for prevention and treatment of diarrhea. | probiotics have been extensively studied over the past several years in the prevention and, to a larger extent, in the treatment of diarrheal diseases, especially in pediatric populations. diarrhea is a symptom, and not a disease. this review will not address chronic disorders associated with diarrhea, or clostridium difficile-induced diarrhea. rather it will focus on published clinical trials performed on acute-onset, likely infectious diarrhea occurring in the settings of day-care centers, in ... | 2011 | 21992955 |
| lack of correlation between virus barosensitivity and the presence of a viral envelope during inactivation of human rotavirus, vesicular stomatitis virus, and avian metapneumovirus by high-pressure processing. | high-pressure processing (hpp) is a nonthermal technology that has been shown to effectively inactivate a wide range of microorganisms. however, the effectiveness of hpp on inactivation of viruses is relatively less well understood. we systematically investigated the effects of intrinsic (ph) and processing (pressure, time, and temperature) parameters on the pressure inactivation of a nonenveloped virus (human rotavirus [hrv]) and two enveloped viruses (vesicular stomatitis virus [vsv] and avian ... | 2011 | 22003028 |
| detection of human g10 rotavirus strains with similarity to bovine and bovine-like equine strains from untypable samples. | g10 rotaviruses, which are usually found in cattle, have also been reported in neonatal infections in recent years. during the rotavirus surveillances of children less than 4years of age between 2003 and 2006 in kolkata, eastern india, 60 out of 1153 samples could not be typed. all 60 samples gave usual electropherotype pattern in polyacrylamide gel. thirty-one out of these 60 g and p untypable rotavirus strains were successfully characterized during the study. among 31 samples, g9p[4] (n=8), g1 ... | 2011 | 22155585 |
| prevalence of human rotavirus genotypes in wuhan, china, during 2008-2011: changing trend of predominant genotypes and emergence of strains with the p[8]b subtype of the vp4 gene. | hospital-based surveillance of rotavirus genotypes was conducted in wuhan, china, between march 2008 and may 2011. the detection rates of group a rotavirus were 24.6% (458/1859) and 12.1% (96/795) in children and adults, respectively, with diarrhea. among the 554 positive specimens, the most frequent genotype was g3p[8] (57.9%), followed by g1p[8] (29.4%). compared with previous studies in wuhan (2000-2008), the relative frequency of g3p[8] has been decreasing year by year, while the predominant ... | 2011 | 21986895 |
| hospitalizations due to rotavirus gastroenteritis in catalonia, spain, 2003-2008. | abstract: | 2011 | 22013948 |
| socio-economic modelling of rotavirus vaccination in castilla y leon, spain. | rotavirus is one of the main causes of acute gastroenteritis in infants and young children. furthermore, rotavirus is the leading cause of hospitalization and death from acute gastroenteritis among infants and young children worldwide. although death due to rotavirus is rare in industrialized regions such as spain, the rotavirus disease burden and its economic impact is severe. this study aims to assess systematic vaccination against rotavirus economically and socially in a spanish region. econo ... | 2011 | 22037437 |
| Infant rotavirus vaccination may provide indirect protection to older children and adults in the United States. | Following the introduction of rotavirus vaccination in the United States, rotavirus and cause-unspecified gastroenteritis discharges significantly decreased in 2008 in the 0-4, 5-14, and 15-24-year age groups, with significant reductions observed in March, the historic peak rotavirus month, in all age groups. We estimate that 15% of the total 66 000 averted hospitalizations and 20% of the $204 million in averted direct medical costs attributable to the vaccination program were among unvaccinated ... | 2011 | 21878425 |
| Oral immunoglobulin for the treatment of rotavirus diarrhea in low birth weight infants. | Rotavirus infection is the most common neonatal nosocomial viral infection. It is a major health problem worldwide. Epidemics with the newer P(6)G9 strains have been reported in neonatal units globally. These strains can cause severe symptoms in most infected infants. Infection control measures become necessary and the utilization of hospital resources increase. Local mucosal immunity in the intestine to rotavirus is important in the resolution of infection and protection against subsequent infe ... | 2011 | 21975740 |
| the african vaccine-preventable diseases network: a vaccine advocacy initiative. | achieving high and equitable childhood immunisation coverage in africa will not only protect children from disability and premature death, it will also boost productivity, reduce poverty and support the economic growth of the continent. thus, africa needs innovative and sustainable vaccine advocacy initiatives. one such initiative is the african vaccine-preventable diseases network, formed in 2009. this association of immunisation practitioners, vaccinologists, paediatricians, and infectious dis ... | 2011 | 22121433 |
| cost-effectiveness of a pentavalent human-bovine reassortant rotavirus vaccine for children ≤5 years of age in taiwan. | a markov model was used to assess the impact of rv5, a pentavalent (g1, g2, g3, g4, p1a[8]) human bovine (wc3 strain) reassortant rotavirus vaccine, on reducing the healthcare burden and cost associated with rotavirus gastroenteritis (rge) in taiwan. other cost-effectiveness analyses for rotavirus vaccination in industrialized countries have produced varying results depending on the input parameters assumed. | 2011 | 21919673 |
| [rotavirus vaccine]. | rotavirus is the single main cause of severe acute gastroenteritis in children less than 5 years of age, resulting in over 527,000 deaths worldwide annually. the majority of rotavirus-related deaths are seen in less-developed countries; in developed countries, rotavirus is an infrequent cause of death but a commom cause of hospitalizations. rotavirus gastroenteritis was also estimated to result in approximately 790,000 doctor visits every year leading to medical intervention among children aged ... | 2011 | 21922762 |
| toxic megacolon. | toxic megacolon represents a dreaded complication of mainly inflammatory or infectious conditions of the colon. it is most commonly associated with inflammatory bowel disease (ibd), i.e., ulcerative colitis or ileocolonic crohn's disease. lately, the epidemiology has shifted toward infectious causes, specifically due to an increase of clostridium difficile-associated colitis possibly due to the extensive (ab)use of broad-spectrum antibiotics. other important infectious etiologies include salmone ... | 2011 | 22009735 |
| the rotavirus enterotoxin (nsp4) promotes re-modeling of the intracellular microtubule network. | expression of the rotavirus enterotoxin (nsp4) in transfected monkey kidney cells was found to result in a dramatic re-modeling of the microtubule (mt) network. this important centrosome organized cytoskeletal element was dissolved by expression of nsp4 and re-formed in a ring array at the periphery of the cell, similar to that seen following normal virus infection. site directed mutagenesis of the n-linked glycosylation sites in nsp4 was employed to show that glycosylation of nsp4 was not requi ... | 2011 | 22036730 |
| determination of bovine rotavirus g serotype by polymerase chain reaction. | a total of 113 diarrheic samples comprising of 68 buffalo calves and 45 cow calves were screened by rna-page for the detection of presence of rotavirus. rna-page analysis of these samples revealed 11 (9.73%) was found positive for rotavirus. out of 68 faecal samples of buffalo calves tested for viral gastroenteritis, 8 (11.76%) were found positive for rotavirus. similarly, out of 45 faecal samples of cattle calves tested for viral gastroenteritis, 3 (6.66%) was found positive for rotavirus. rota ... | 2011 | 21892766 |
| genetic analyses reveal differences in the vp7 and vp4 antigenic epitopes between human rotaviruses circulating in belgium and rotaviruses in rotarix™ and rotateq™ | two live-attenuated rotavirus group a (rva) vaccines, rotarix™ (g1p[8]) and rotateq™ (g1-g4, p[8]), have been successfully introduced in many countries worldwide, including belgium. the parental rva strains used to generate the vaccines were isolated more than 20 years ago in france (g4 parental strain in rotateq™) and the united states (all other parental strains). at present, little is known about the relationship between currently circulating human rvas and the vaccine strains. in this study, ... | 2011 | 22189107 |
| Usefulness of fecal lactoferrin in predicting and monitoring the clinical severity of infectious diarrhea. | To explore the value of fecal lactoferrin in predicting and monitoring the clinical severity of infectious diarrhea. | 2011 | 22072854 |
| [Application of clinical pathways in children with Rotavirus enteritis]. | Clinical pathways are standardized, multidisciplinary, integrated management plans. This study aimed to evaluate the efficacy of clinical pathways in children with Rotavirus enteritis. | 2011 | 22000440 |
| Changing patterns of rotavirus genotypes in Turkey. | To describe the circulation dynamics of human rotavirus genotypes and to understand the epidemiological changes of rotavirus infection in Turkey, one of the countries where the highest mortality rates are seen due to rotavirus in Europe. Stool samples of children under 5 years of age which gave positive results for rotavirus antigen were stored at -20°C and then genotyped using multiplex reverse transcription polymerase-chain reaction. Of the 494 stool samples, 137 (28.1%) were positive for rota ... | 2011 | 21938522 |
| In situ gastrointestinal protection against anthrax edema toxin by single-chain antibody fragment producing lactobacilli. | ABSTRACT: BACKGROUND: Anthrax is caused by the bacterium Bacillus anthracis and is regarded as one of the most prominent bioterrorism threats. Anthrax toxicity is induced by the tripartite toxin complex, composed of the receptor-binding anthrax protective antigen and the two enzymatic subunits, lethal factor and edema factor. Recombinant lactobacilli have previously been used to deliver antibody fragments directed against surface epitopes of a variety of pathogens, including Streptococcus mutan ... | 2011 | 22185669 |
| genetic diversity of group a rotaviruses associated with repeated outbreaks of diarrhea in a farrow-to-finish farm: identification of a porcine rotavirus strain bearing a novel vp7 genotype, g26. | abstract: group a rotaviruses (gars) are one of the most common causes of diarrhea in suckling pigs. although a number of g and p genotypes have been identified in porcine gars, few attempts have been made to study the molecular epidemiology of these viruses associated with diarrhea outbreaks within a farm over an extended period of time. here, we investigated the molecular characteristics of gars that caused four outbreaks of diarrhea among suckling pigs in a farrow-to-finish farm over the cour ... | 2011 | 22067072 |
| effects of lactoferrin-containing formula in the prevention of enterovirus and rotavirus infection and impact on serum cytokine levels: a randomized trial. | lactoferrin has been shown to exhibit anti-enterovirus 71 (ev71) and antirotavirus properties. this trial was conducted to determine whether a formula containing bovine lactoferrin (blf) exerts a protective effect against ev71 or rotavirus infection among children from 2 to 6 years old. | 2011 | 21880194 |
| detection of enterovirus 71 using reverse transcription loop-mediated isothermal amplification (rt-lamp). | reverse transcription loop-mediated isothermal amplification (rt-lamp), which is a visual assay for nucleic acids, is performed in a single step using one tube at 65°c for 1.5h. in this study, rt-lamp was established as a method for the detection of enterovirus 71 (ev71). the detection limit of the assay was approximately 10 copies, and no cross-reactivity was noted with coxsackievirus a16, echovirus, human rotavirus (hrv) or norovirus. this assay, which offers greater sensitivity at a lower cos ... | 2011 | 22155579 |
| the role of type-2 turkey astrovirus in poult enteritis syndrome. | an experimental study was conducted to determine the comparative pathogenicity of type-2 turkey astrovirus (tastv-2) obtained from turkey flocks afflicted with poult enteritis syndrome (pes) and from turkey flocks displaying no apparent signs of infection. in total, ninety 7-d-old poults, which tested negative for the presence of astrovirus, rotavirus, coronavirus, and reovirus by reverse transcriptase (rt) pcr , were divided evenly into 3 groups: a, b, and c. birds in group a were inoculated or ... | 2011 | 22080013 |
| Novel structural insights into rotavirus recognition of ganglioside glycan receptors. | Rotaviruses ubiquitously infect children under the age of 5, being responsible for more than half a million diarrhoeal deaths each year worldwide. Host cell oligosaccharides containing sialic acid(s) are critical for attachment by rotaviruses. However, to date, no detailed three-dimensional atomic model showing the exact rotavirus interactions with these glycoconjugate receptors has been reported. Here, we present the first crystallographic structures of the rotavirus carbohydrate-recognizing pr ... | 2011 | 21945555 |
| acute motor-sensory axonal guillain-barré syndrome with unilateral facial nerve paralysis after rotavirus gastroenteritis in a 2-year-old boy. | guillain-barré syndrome (gbs) is well known as presenting with acute immune-mediated polyneuropathies, with strong associations with antecedent infections. several variant forms of gbs have been described, including acute inflammatory demyelinating polyneuropathy, acute motor axonal neuropathy, acute motor-sensory axonal neuropathy, and sensory gbs. we present a rare case of 2-year-old boy with acute motor and sensory polyneuropathy and left-sided facial nerve paralysis after rotavirus infection ... | 2011 | 21915637 |
| [infectious enteritis]. | infectious diarrhea belongs to the most frequent infections worldwide and can be elicited by a wide array of microbial pathogens. in developed countries transmission occurs much more frequently from contaminated food as compared to direct person-to-person contact, except for enteric viruses which can also be transmitted by aerosol formation after vomiting. in germany, more than 90% of cases are caused by the four pathogens norovirus, rotavirus, campylobacter and salmonella. therapy of infectious ... | 2011 | 21847579 |
| post marketing evaluation of the short-term safety of the pentavalent rotavirus vaccine. | background: a pentavalent rotavirus vaccine (rv5) demonstrated efficacy and safety in a large clinical trial before us licensure in 2006. the primary objective of this observational study was to assess the occurrence of intussusception (is) among infants who received rv5 in routine use. secondary objectives assessed the occurrence of kawasaki disease (kd) and general safety. methods: we identified and followed infants with a health insurance claim for rv5 during the first 2 years of rv5 availabi ... | 2011 | 22173146 |
| Silencing of the rotavirus NSP4 protein decreases the incidence of biliary atresia in murine model. | Biliary atresia is a common disease in neonates which causes obstructive jaundice and progressive hepatic fibrosis. Our previous studies indicate that rotavirus infection is an initiator in the pathogenesis of experimental biliary atresia (BA) through the induction of increased nuclear factor-kappaB and abnormal activation of the osteopontin inflammation pathway. In the setting of rotavirus infection, rotavirus nonstructural protein 4 (NSP4) serves as an important immunogen, viral protein 7 (VP7 ... | 2011 | 21876759 |
| high prevalence of community-acquired norovirus gastroenteritis among hospitalized children: a prospective study. | acute gastroenteritis (age) causes significant morbidity, especially in young children, and frequently requires hospitalization even in developed countries. surveillance studies of age are important to determine the prevalence and variety of bacterial and viral pathogens, to initiate targeted preventive measures, such as vaccine programmes, and to monitor its impact. a prospective study was conducted in children <5 years old, admitted with age between april 2006 and april 2007 to the virgen del ... | 2011 | 21848976 |
| Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes. | Edible fruits are inexpensive biofactories for human health-promoting molecules that can be ingested as crude extracts or partially purified formulations. We show here the production of a model human antibody for passive protection against the enteric pathogen rotavirus in transgenically labelled tomato fruits. Transgenic tomato plants expressing a recombinant human immunoglobulin A (hIgA_2A1) selected against the VP8* peptide of rotavirus SA11 strain were obtained. The amount of hIgA_2A1 protei ... | 2011 | 22070155 |
| Sapovirus as a gastrointestinal pathogen in febrile pediatric patients with cancer. | Human caliciviruses are the second most common cause of viral gastroenteritis after rotavirus in children. Unlike norovirus, sapovirus infection is less well characterized and defined in the clinical setting of gastrointestinal disease, and there are no reports of sapovirus infections in pediatric oncology patients receiving chemotherapy treatment. Stool samples from all pediatric oncology patients presenting with fever and diarrhea at one pediatric oncology unit were tested prospectively for sa ... | 2011 | 22012734 |
| Decreasing Rate of Biliary Atresia in Taiwan: A Survey, 2004-2009. | Objectives: The pathogenesis of biliary atresia (BA) is unclear, but epidemiological studies may help to elucidate possible causes. The goals of this study were to identify BA incidence changes in Taiwan in 2004-2009 and to survey the factors that might influence incidence changes to elucidate the possible causes of BA. Methods: A Taiwan national registry system for BA has been established since 2004. By using data from the national registry system for BA, we identified BA incidence changes in 2 ... | 2011 | 21873702 |
| the cost-effectiveness of rotavirus vaccination in armenia. | the cost-effectiveness of introducing infant rotavirus vaccination in armenia in 2012 using rotarix(r) was evaluated using a multiple birth cohort model. the model considered the cost and health implications of hospitalisations, primary health care consultations and episodes not leading to medical care in children under five years old. rotavirus vaccination is expected to cost the ministry of health $220,000 in 2012, rising to $830,000 in 2016 following termination of gavi co-financing, then dec ... | 2011 | 21945959 |
| applications of pcr (real-time and masstag) and enzyme-linked immunosorbent assay in diagnosis of respiratory infections and diarrheal illness among deployed u.s. military personnel during exercise balikatan 2009, philippines. | laboratory-based surveillance for diarrheal and respiratory illness was conducted at the 2009 republic of the philippines-united states balikatan exercise to determine the presence of specific pathogens endemic in the locations where the military exercises were conducted. ten stool and 6 respiratory specimens were obtained from individuals meeting case definitions for diarrhea or respiratory illness. stool specimens were frozen in dry ice and remotely tested using enzyme-linked immunosorbent ass ... | 2011 | 22128641 |
| First description of group A rotavirus from fecal samples of ostriches (Struthio camelus). | This study investigated the occurrence of rotavirus infections in ostriches (Struthio camelus) reared in Northern Paraná, Brazil. Fecal (n=66) and serum (n=182) samples from nine farms located in four different cities were analyzed by silver stained-polyacrylamide gel electrophoresis (ss-PAGE), RT-PCR assay, virus isolation, and counterimmunoelectroosmophoresis (CIE). Rotavirus group A seropositivity occurred in 5.49% (10/182) of serum samples of ostriches originated from two farms. Only 9.09% ( ... | 2011 | 22209018 |
| Novel pentameric structure of the diarrhea-inducing region of the rotavirus enterotoxigenic protein NSP4. | A novel pentameric structure which differs from the previously reported tetrameric form of the diarrhea-inducing region of the rotavirus enterotoxin NSP4 is reported here. A significant feature of this pentameric form is the absence of the calcium ion located in the core region of the tetrameric structures. The lysis of cells, the crystallization of the region spanning residues 95 to 146 of NSP4 (NSP4(95-146)) of strain ST3 (ST3:NSP4(95-146)) at acidic pH, and comparative studies of the recombin ... | 2011 | 21917949 |
| Regulation of gene expression by the NSP1 and NSP3 non-structural proteins of rotavirus. | The role of the rotavirus non-structural proteins NSP1 and NSP3 in regulating cellular and viral mRNA translation has been investigated by examining the effect of added recombinant NSP3 on protein translation in a T7-based in vitro coupled transcription-translation system. Addition of purified NSP3 to assays primed solely with cellular mRNA was found to have no effect on the translation efficiency of the mRNA. However, as expected, the addition of viral mRNA to such assays competitively inhibite ... | 2011 | 21947567 |
| receptor tyrosine kinase inhibitors that block replication of influenza a and other viruses. | we have previously reported that two receptor tyrosine kinase inhibitors (rtkis), called ag879 and tyrphostin a9 (a9), can each block the replication of influenza a virus in cultured cells. in this study, we further characterized the in vitro antiviral efficacies and specificities of these agents. the 50% effective concentration (ec(50)) of each against influenza a was found to be in the high nanomolar range, and the selectivity index (si = 50% cytotoxic concentration [cc(50)]/ec(50)) was determ ... | 2011 | 21930873 |
| characterization of norovirus strains in australian children from 2006 to 2008: prevalence of recombinant strains. | noroviruses are highly infectious and are the most common cause of gastroenteritis outbreaks. genotype ii.4 strains have been the dominant type identified in adults, however the genotype distribution in children is less clearly defined. this study aimed to detect and genotype norovirus strains infecting children hospitalized with acute gastroenteritis in melbourne, australia from 2006 to 2008. stool samples were collected from 272 children admitted to the royal children's hospital, melbourne, au ... | 2011 | 22012731 |
| an assay for detecting neutralization of rotavirus infection by quantitative determination of vp6 protein fluorescence intensity. | previous rotavirus infection studies used the focus reduction assay extensively to evaluate cellular responses to viral infection, but this technique has a number of limitations. in this study, we developed a simplified, accurate rotavirus infection assay to evaluate the effects of inhibitory substances on rotavirus infection in vitro by measurement of the fluorescence intensities of stained cells. | 2011 | 21979094 |
| Norovirus prevalence in 'pathogen negative' gastroenteritis in children from periurban areas in Lima, Peru. | Norovirus was detected in 17.4% of 224 diarrhoeal samples from children younger than 24 months of age in Lima, in whom all common pathogens had been excluded (pathogen negative). Norovirus was identified more frequently in children older than 12 months of age than in younger children (34% vs 8%, P<0.001). Among norovirus-positive samples, genogroup II was the predominant group (92%). Compared with rotavirus, norovirus episodes tended to be of shorter duration and less severe. The role of norovir ... | 2011 | 21962615 |
| cross-linking of rotavirus outer capsid protein vp7 by antibodies or disulfides inhibits viral entry. | antibodies that neutralize rotavirus infection target outer coat proteins vp4 and vp7 and inhibit viral entry. the structure of a vp7-fab complex (s. t. aoki, et al., science 324:1444-1447, 2009) led us to reclassify epitopes into two binding regions at inter- and intrasubunit boundaries of the calcium-dependent trimer. it further led us to show that antibodies binding at the intersubunit boundary inhibit uncoating of the virion outer layer. we have now tested representative antibodies for each ... | 2011 | 21849465 |
| immunization schedule of the spanish association of pediatrics: 2012 recommendations. | the advisory committee on vaccines of the spanish association of pediatrics (cav-aep) updates the immunization schedule every year, taking into account epidemiological data as well as evidence on the effectiveness and efficiency of vaccines. the present schedule includes grades of recommendation. we have graded as routine vaccinations those that the cav-aep believes all children should receive; as recommended those that fit the profile for universal childhood immunization and would ideally be gi ... | 2011 | 22177960 |
| [Detection and genotyping of rotavirus in diarrhea infant feces in Chengdu]. | To study the genotype of rotavirus and the genetic variations of the major neutralization antigen VP4 of group A rotavirus in fecal samples from infants with diarrhea in Chengdu, Sichuan province, China. | 2011 | 21866637 |
| Novel Probiotic Bifidobacterium longum subsp. infantis CECT 7210 Strain Active against Rotavirus Infections. | Rotavirus is the leading cause of severe acute gastroenteritis among children worldwide. It is well known that breast-feeding and vaccination afford infants protection. Since breast-feeding has drastically decreased in developed countries, efforts have been focused on the potential use of probiotics as preventive agents. In this study, a novel Bifidobacterium longum subsp. infantis strain was isolated from infant feces and selected, based on its capacity to inhibit in vitro rotavirus Wa replicat ... | 2011 | 22003027 |
| suspected zoonotic transmission of rotavirus group a in danish adults. | summarygroup a rotaviruses infect humans and a variety of animals. in july 2006 a rare rotavirus strain with g8p[14] specificity was identified in the stool samples of two adult patients with diarrheoa, who lived in the same geographical area in denmark. nucleotide sequences of the vp7, vp4, vp6, and nsp4 genes of the identified strains were identical. phylogenetic analyses showed that both danish g8p[14] strains clustered with rotaviruses of animal, mainly, bovine and caprine, origin. the high ... | 2011 | 21943834 |
| impact of introducing the pneumococcal and rotavirus vaccines into the routine immunization program in niger. | objectives. we investigated whether introducing the rotavirus and pneumococcal vaccines, which are greatly needed in west africa, would overwhelm existing supply chains (i.e., the series of steps required to get a vaccine from the manufacturers to the target population) in niger. methods. as part of the bill and melinda gates foundation funded vaccine modeling initiative, we developed a computational model to determine the impact of introducing these new vaccines to niger's expanded programs on ... | 2011 | 21940923 |
| early observations in the use of oral rotavirus vaccination in infants with functional short gut syndrome. | aim: universal vaccination with an oral live-attenuated rotavirus vaccine at 2, 4 and 6 months of age was introduced in australia in july 2007. there are no data on the short-term effects of vaccination for those infants most at risk of severe complications from rotavirus infection. the aim of this study was to describe the effects of rotavirus vaccination on weight gain and gastrointestinal losses in infants with functional short gut syndrome secondary to an ileostomy. methods: a retrospectiv ... | 2011 | 22107074 |
| characteristics of rotavirus gastroenteritis in hospitalized children in israel. | diarrhea is a leading cause of child mortality worldwide. rotavirus is one of the most common causes of severe diarrhea and dehydration in children. | 2011 | 21845967 |
| report of the second european expert meeting on rotavirus vaccination. | 2011 | 22178724 | |
| Full genomic analyses of human rotavirus G4P[4], G4P[6], G9P[19] and G10P[6] strains from North-eastern India: evidence for interspecies transmission and complex reassortment events. | In hospitalized patients with acute gastroenteritis in Manipur, India, four rotavirus strains were found to possess VP7 and/or VP4 genes with porcine or bovine characteristics. Considering the animal-like nature of these strains, the remaining eight gene segments were analysed to decipher their exact origin. Analyses of full genome of these strains exhibited their origin from porcine/bovine rotaviruses. This study suggests single or multiple events of reassortment involving multiple gene segment ... | 2011 | 21884295 |
| Rapid and sensitive detection of PRRSV by a reverse transcription-loop-mediated isothermal amplification assay. | A real-time monitoring reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the sensitive and specific detection of prototypic, prevalent North American porcine reproductive and respiratory syndrome virus (PRRSV) strains. As a higher sensitivity and specificity method than reverse transcription polymerase chain reaction (RT-PCR), the RT-LAMP method only used a turbidimeter, exhibited a detection limit corresponding to a 10(-4) dilution of template RNA ex ... | 2011 | 21847756 |