Publications

restriction fragment analysis of the temporal program of bacteriophage spo1 transcription and its control by phage-modified rna polymerases.		1977	412317
streptomycin-resistant, asporogenous mutant of bacillus subtilis.	a streptomycin-resistant mutantant of bacillus subtilis that is also asporogenous, was isolated and partially characterized. this strain, srb15, sporulated at a frequency of about 1% compared to the wild type frequency of greater than 70%. the two phenotypes were inseparable by transformation, suggesting that this strain carries a single mutation that causes it to be both streptomycin-resistant and spore-minus. the mutation cotransduces with cysa, the closest auxotrophic marker to the "ribosomal ...	1977	414073
regulatory gene 28 of bacteriophage spo1 codes for a phage-induced subunit of rna polymerase.		1976	815553
the virus-specified subunits of a modified b. subtilis rna polymerase are determinants of dna binding and rna chain initiation.	the phage spo1-modified rna polymerase b-p can form rapidly initiating complexes with spo1 dna but not with heterologous phi1 dna. the b-p enzyme binds only weakly to heterologous phi29 dna: preincubation with phi29 dna does not substantially slow the formation of rapidly initiating complexes between polymerase b-p and subsequently added spo1 dna. in contrast, b. subtilis holoenzyme and core polymerase are substantially sequestered by preincubation with phi29 dna. the results show that at least ...	1976	821619
bacteriophage sp01 regulatory proteins directing late gene transcription in vitro.		1976	822348
immunogenic properties of bacteriophage spo1 and t4 dna photooxidized in the presence of methylene blue, irradiated with ultra-violet light or containing 5-bromdesoxyuridine.		1976	1254318
bacteriophage spo1-induced macromolecular synthesis in minicells of bacillus subtilis.	spo1 bacteriophage injects its dna into minicells produced by bacillus subtilis cu403 divivb1. the injected dna is partially degraded to small trichloracetic acid-precipitable material and trichloroacetic acid-soluble material. the injected dna is not replicated; however, it serves as a template for rna and protein synthesis. the rna produced specifically hybridizes to spo1 dna, and the amount of rna hybridized can be reduced by competition with rna isolated at all stages of the phage cycle from ...	1975	806703
bacteriophage spo1 dna- and rna-directed protein synthesis in vitro: the effect of tf1, a template-selective transcription inhibitor.	transcription factor one (tf1), a protein synthesized after infection of b. subtilis by phage spo1, is a specific inhibitor of spo1 in vitro transcription. in this paper, we investigate the effect of tf1 on spo1-specific in vitro protein synthesis, using spo1 dna or messenger rna as templates. protein syhthesis is measured by incorporation of radioactive amino acids into acid insoluble form, synthesis of a phage-specific enzymatic activity, and analysis of radioactive polypeptides by acrylamide ...	1975	810655
bacteriophage spo1 dna- and rna-directed protein synthesis in vitro: comparison with in vivo control.	a cell free protein synthesizing system, derived from e. coli, is shown to be a quantitiative assay system for messenger rna extracted from b. subtilis infected with bacteriophage spo1. dna-directed protein synthesis in this system is shown to be limited mostly to those proteins whose messages are contained in early rna. a phage induced enzyme, dcmp deaminase, is shown to be dependent on appearance of a class mrna made in vivo in response to new initiations of transcription dependent on prior sy ...	1975	810656
synthesis of specific functional messenger rna in vitro by phage-sp01-modified rna polymerase of bacillus subtilis.	rna polymerase (nucleosidetriphosphate: rna nucleotidyltransferase, ec 2.7.7.6) was purified from rifampicin-resistant bacillus subtilis, from both uninfected cells and cells infected with bacteriophage sp01. the enzyme from infected cells lacked all traces of the sigma subunit, contained several polypeptides absent from the enzyme made in uninfected cells, and had an altered template specificity in a transcription assay. a cell-free protein synthesizing system from escherichia coli, when poison ...	1975	813216
bacillus subtilis bacteriophage sp01, sp82, and phi e require host lysyl- and tryptophanyl-trna synthetases for phage development.	infection of bacillus subtilis mutants having temperature-sensitive lysyl- or tryptophanyl-trna synthetases with bacteriophage sp01, sp82, or phie indicates that both host enzymes are essential for phage development. no apparent modification of the temperature-sensitive phenotype of the mutant host enzymes occurs during phage infection.	1974	4211168
new phage-spo1-induced polypeptides associated with bacillus subtilis rna polymerase.	rna polymerase was precipitated from extracts of bacillus subtilis infected with phage sp01 by antiserum prepared against core rna polymerase. as shown by sodium dodecyl sulfate gel electrophoresis, the precipitates contained at least five new polypeptides not present in uninfected bacteria, in addition to the known subunits of rna polymerase. the molecular weights of these polypeptides are (1) 85,000; (ii) 40,000; (iii) 28,000; (iv) 25,000; and (v) 23,000. four of the polypeptides (i, iii, iv, ...	1974	4212197
purification of tf1--a template-specific dna-binding protein and transcription inhibitor from bacteriophage spo1-infected bacillus subtilis.		1974	4212291
deoxyribonucleic acid synthesis in bacteriophage sp01-infected bacillus subtilis. ii. purification and catalytic properties of a deoxyribonucleic acid polymerase induced after infection.		1973	4200923
transcription specificity of an rna polymerase fraction from bacteriophage sp01-infected b. subtilis.		1973	4201173
minicells of bacillus subtilis: a new bacteriophage-blocking agent.	bacteriophage sp01, sp17, and phi29 rapidly absorb irreversibly to minicells of bacillus subtilis but do not produce a lytic cycle in minicells.	1973	4204693
deoxyribonucleic acid synthesis in bacteriophage spo1-infected bacillus subtilis. i. bacteriophage deoxyribonucleic acid synthesis and fate of host deoxyribonucleic acid in normal and polymerase-deficient strains.	the effect of bacteriophage spo1 infection of bacillus subtilis and a deoxyribonucleic acid (dna) polymerase-deficient (pol(-)) mutant of this microorganism on the synthesis of dna has been examined. soon after infection, the incorporation of deoxyribonucleoside triphosphates into acid-insoluble material by cell lysates was greatly reduced. this inhibition of host dna synthesis was not a result of host chromosome degradation nor did it appear to be due to the induction of thymidine triphosphate ...	1972	4622590
specific inhibition of bacteriophage spo1 dna-directed protein synthesis by the spo1 transcription factor, tf 1.		1972	4622613
purification of the bacteriophage sp01 transcription factor 1.		1972	4624121
the genetics of bacteriophage spo1.		1972	4628367
immunogenic specificity of bacillus subtilis phage spo1-dna.		1971	4107659
rna synthesis during bacteriophage spo1 development: six classes of spo1 rna.		1971	4996233
transcription during bacteriophage spo1 development: mutations affecting the program of viral transcription.		1971	4996235
effect of nalidixic acid on deoxyribonucleic acid synthesis in bacteriophage spo1-infected bacillus subtilis.	the effect of nalidixic acid on deoxyribonucleic acid (dna) synthesis in bacillus subtilis cells infected with bacteriophage spo1 was studied. nalidixic acid had little inhibitory effect on spo1 dna synthesis at concentrations that drastically inhibited b. subtilis dna synthesis. inhibition of dna synthesis, appropriate to the concentration used, was imposed within 1 min after addition of nalidixic acid, suggesting that it acts directly on dna synthesis in both infected and uninfected cells. the ...	1969	4976473
a template-selective inhibitor of in vitro transcription.	a macromolecular factor, tf1, has been isolated from bacteriophage sp01-infected b. subtilis. tf1 selectively inhibits in vitro transcription of sp01 and related viral dna. evidence is presented regarding these properties of the repressor-like tf1: (1) template and conformational specificity; (2) interaction with dna rather than rna polymerase; (3) trypsin sensitivity; (4) reversibility of action; and (5) ability to block initiation, but not propagation, of rna synthesis.	1969	4978743