Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| immune responses of chickens inoculated with a recombinant fowlpox vaccine coexpressing ha of h9n2 avain influenza virus and chicken il-18. | control of the circulation of h9n2 avian influenza virus (aiv) is a major concern for both animal and public health, and h9n2 aiv poses a major threat to the chicken industry worldwide. here, we developed a recombinant fowlpox virus (rfpv-ha) expressing the haemagglutinin (ha) gene of the a/ch/jy/1/05 (h9n2) influenza virus and a recombinant fowlpox virus (rfpv-ha/il18) expressing the ha gene and chicken interleukin-18 (il-18) gene. recombinant plasmid psy-ha/il18 was constructed by cloning chic ... | 2011 | 21549153 |
| differential effects of viral vectors on migratory afferent lymph dendritic cells in vitro predicts enhanced immunogenicity in vivo. | targeting dendritic cells (dc) is key to driving effective immune responses. lymphatic cannulation provides access to the heterogeneous populations of dc draining peripheral sites in rodents and ruminants. afferent lymph dec-205(+)cd11c(+)sirpa(+) dc were preferentially infected ex vivo with three vaccine viral vectors: replication defective human adenovirus-5 (rhuadv5); modified vaccinia virus ankara (rmva) and fowlpox virus (rfpv) all expressing green fluorescent protein (gfp). the rhuadv5 inf ... | 2011 | 21752909 |
| detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in india. | fowl poxvirus (fpv) infection was observed in unvaccinated backyard chickens. a total of 15 birds were affected in a flock of 37. pock lesions were observed on the comb, eyelids, beak and wattles. the birds appeared sick with roughened feathers and stunted growth. no mortality was recorded. dna was isolated from scabs and polymerase chain reaction (pcr) was performed to amplify the 4b core protein gene of fpv, the envelope (env) gene of reticuloendotheliosis virus (rev) and the region of fpv fla ... | 2011 | 21706467 |
| Innate immune recognition of poxviral vaccine vectors. | The study of poxviruses pioneered the field of vaccinology after Jenner's remarkable discovery that 'vaccination' with the phylogenetically related cowpox virus conferred immunity to the devastating disease of smallpox. The study of poxviruses continues to enrich the field of virology because the global eradication of smallpox provides a unique example of the potency of effective immunization. Other poxviruses have since been developed as vaccine vectors for clinical and veterinary applications ... | 2011 | 21988308 |
| delivery of a multivalent scrambled antigen vaccine induces broad spectrum immunity and protection against tuberculosis. | the development of effective anti-tuberculosis (tb) vaccines is an important step towards improved control of tb in high burden countries. subunit vaccines are advantageous in terms of safety, particularly in the context of high rates of hiv co-infection, but they must contain sufficient mycobacterium tuberculosis antigens to stimulate immunity in genetically diverse human populations. we have used a novel approach to develop a synthetic scrambled antigen vaccine (tb-savine), comprised of overla ... | 2011 | 21846485 |
| construction and characterisation of a recombinant fowlpox virus that expresses the human papilloma virus l1 protein. | human papilloma virus (hpv)-16 is the most prevalent high-risk mucosal genotype. virus-like-particle (vlp)-based immunogens developed recently have proven to be successful as prophylactic hpv vaccines, but are still too expensive for developing countries. although vaccinia viruses expressing the hpv-16 l1 protein (hpv-l1) have been studied, fowlpox-based recombinants represent efficient and safer vectors for immunocompromised hosts due to their ability to elicit a complete immune response and th ... | 2011 | 22053827 |
| heterologous prime-boost-boost immunisation of chinese cynomolgus macaques using dna and recombinant poxvirus vectors expressing hiv-1 virus-like particles. | there is renewed interest in the development of poxvirus vector-based hiv vaccines due to the protective effect observed with repeated recombinant canarypox priming with gp120 boosting in the recent thai placebo-controlled trial. this study sought to investigate whether a heterologous prime-boost-boost vaccine regimen in chinese cynomolgus macaques with a dna vaccine and recombinant poxviral vectors expressing hiv virus-like particles bearing envelopes derived from the most prevalent clades circ ... | 2011 | 21899739 |
| gene expression profiling in rmd5- and rmd5deltameq-infected chickens. | marek's disease (md) is a lymphoproliferative disorder of domestic chickens caused by a highly contagious and oncogenic alpha-herpesvirus, marek's disease virus (mdv). md is characterized by bursal-thymic atrophy and rapid onset of t-cell lymphomas that infiltrate lymphoid tissues, visceral organs, and peripheral nerves with severe clinical signs that include transient paralysis, anemia, weight loss, and neurologic disorders. using overlapping cosmids- and bac-cloned mdv, it has been shown that ... | 2011 | 22017031 |
| a pilot safety trial investigating a vector-based vaccine targeting carcinoembryonic antigen in combination with radiotherapy in patients with gastrointestinal malignancies metastatic to the liver. | previous studies have demonstrated the ability of non-lethal doses of radiation to alter the phenotype of tumor cells to facilitate immune mediated killing. this pilot study evaluated the tolerability of a vector-based vaccine targeting carcinoembryonic antigen (cea) in combination with radiation therapy in patients with gastrointestinal malignancies metastatic to the liver. | 2011 | 21871012 |
| outbreak of cutaneous form of poxvirus on a commercial turkey farm caused by the species fowlpox. | the present report documents the occurrence of a poxvirus infection in commercial meat turkeys. the affected farm had six flocks, with a total of 11,680 birds at different ages; birds from two of these flocks were affected. the clinical picture was characterized by severe epithelial lesions and proliferations on the head and neck regions as reported for the cutaneous form of poxvirus infection. except for these lesions, no adverse clinical signs or gross pathologic lesions were observed. only a ... | 2011 | 22312999 |
| efficacy of avian influenza vaccine in poultry: a meta-analysis. | vaccination is an effective method for controlling avian influenza (ai), especially in countries with endemic infection. this study conducted a bayesian meta-analysis to evaluate the efficacy of ai vaccines in chickens. we included both inactivated and recombinant fowlpox virus expressing h5 (rfpv-h5) vaccine studies that used specific-pathogen-free chickens where outcomes against the h5n1 or h5n2 ai viruses were measured. vaccine efficacy was evaluated by protection from mortality, protection f ... | 2010 | 21313840 |
| protection against infectious laryngotracheitis by in ovo vaccination with commercially available viral vector recombinant vaccines. | infectious laryngotracheitis (ilt) is a highly contagious respiratory disease of chickens caused by infectious laryngotracheitis virus (iltv). the disease is mainly controlled through biosecurity and by vaccination with live-attenuated vaccines. the chicken embryo origin (ceo) vaccines, although proven to be effective in experimental settings, have limited efficacy in controlling the disease in dense broiler production sites due to unrestricted use and poor mass vaccination coverage. these facto ... | 2010 | 21313847 |
| molecular biological characterization of avian poxvirus strains isolated from different avian species. | fifteen strains of avipoxvirus from different avian species were isolated and molecular biologically characterized. most strains did not produce evident pocks on the chorioallantoic membranes of commercial and specific-pathogen free embryonated chicken eggs where, on the contrary, microscopic signs of viral growth were always detected. polymerase chain reaction of highly conserved p4b gene was positive for all cases confirming to be a reliable diagnostic method for avipoxvirus. sequencing of the ... | 2010 | 19646826 |
| protective immune response of chickens to oral vaccination with thermostable live fowlpox virus vaccine (strain tpv-1) coated on oiled rice. | the objective of the present study was to develop and evaluate a local vaccine (strain tpv-1) against fowl pox (fp) in chickens. two separate groups of chickens were vaccinated with fp vaccine through oral (coated on oiled rice) and wing web stab routes, respectively. the results showed that the haemagglutination-inhibition (hi) antibody titres in both vaccinated groups were comparable and significantly higher (p < 0.05) than the control chickens. it was further revealed that 14 days after vacci ... | 2010 | 19714476 |
| characterization of host responses against a recombinant fowlpox virus-vectored vaccine expressing the hemagglutinin antigen of an avian influenza virus. | there currently are commercial fowlpox virus (fpv)-vectored vaccines for use in chickens, including trovac-aiv h5, which expresses the hemagglutinin (ha) antigen of an avian influenza virus and can confer immunity against avian influenza in chickens. despite the use of recombinant fpv (rfpv) for vaccine delivery, very little is known about the immune responses generated by these viruses in chickens. the present study was designed to investigate host responses to rfpv in vivo and in vitro. in cul ... | 2010 | 20071494 |
| severe histiolymphocytic and heterophilic bronchopneumonia as a reaction to in ovo fowlpox vaccination in broiler chicks. | broiler chickens on several farms from a single poultry company experienced neurological signs and mortality in chicks between 3 days and 10 days of age over a 3-week period after use of a fowlpox-vectored infectious laryngotracheitis virus vaccine in ovo. at necropsy the lungs contained numerous tan or gray, opaque to translucent, 0.5- to 2.0-mm nodules in the parenchyma. microscopic lesions were a multifocal severe lymphohistiocytic and heterophilic bronchopneumonia. immunohistochemistry was p ... | 2010 | 20080499 |
| cytokine-armed vaccinia virus infects the mesothelioma tumor microenvironment to overcome immune tolerance and mediate tumor resolution. | intratumoral (i.t.) administration of cytokine genes expressed by viral vectors represents a rational approach that induces cytokine secretion at the site they are needed, and i.t. vaccinia virus (vv) has shown promise in mesothelioma patients. however, we and others have shown that the mesothelioma tumor microenvironment includes macrophages, dendritic cells (dcs), and t cells. therefore, we investigated which of these cell types are infected after exposure to vv or fowlpox virus (fpv)-cytokine ... | 2010 | 20150930 |
| canarypox and fowlpox viruses as recombinant vaccine vectors: an ultrastructural comparative analysis. | due to their natural host-range restriction to avian species, canarypox virus (cp) and fowlpox virus (fp) represent efficient and safe vaccine vectors, as they correctly express transgenes in human cells, elicit complete immune responses, and show protective efficacy in preclinical animal models. at present, no information is available on the differences in the abortive replication of these two avipox viruses in mammalian cells. in the present study, the replicative cycles of cp and fp, wild-typ ... | 2010 | 20379750 |
| metal cofactors in the structure and activity of the fowlpox resolvase. | poxvirus dna replication generates linear concatemers containing many copies of the viral genome with inverted repeat sequences at the junctions between monomers. the inverted repeats refold to generate holliday junctions, which are cleaved by the virus-encoded resolvase enzyme to form unit-length genomes. here we report studies of the influence of metal cofactors on the activity and structure of the resolvase of fowlpox virus, which provides a tractable model for in vitro studies. small-molecul ... | 2010 | 20380839 |
| diagnosis of a naturally occurring dual infection of layer chickens with fowlpox virus and gallid herpesvirus 1 (infectious laryngotracheitis virus). | an outbreak of acute respiratory disease in layers was diagnosed as being of dual nature due to fowlpox and infectious laryngotracheitis using a multidisciplinary approach including virus isolation, histopathology, electron microscopy and polymerase chain reaction (pcr). the diagnosis was based on virus isolation of gallid herpesvirus 1 (gahv-1) in chicken kidney cells and fowlpox virus (fwpv) in 9-day-old chicken embryonated eggs inoculated via the chorioallantoic membrane. the histopathology o ... | 2010 | 20390533 |
| fowlpox virus recombinants expressing hpv-16 e6 and e7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits. | around half million new cases of cervical cancer arise each year, making the development of an effective therapeutic vaccine against hpv a high priority. as the e6 and e7 oncoproteins are expressed in all hpv-16 tumour cells, vaccines expressing these proteins might clear an already established tumour and support the treatment of hpv-related precancerous lesions. | 2010 | 20409340 |
| type i interferons mediate the innate cytokine response to recombinant fowlpox virus but not the induction of plasmacytoid dendritic cell-dependent adaptive immunity. | type i interferons (ifns) are considered to be important mediators of innate immunity due to their inherent antiviral activity, ability to drive the transcription of a number of genes involved in viral clearance, and their role in the initiation of innate and adaptive immune responses. due to the central role of type i ifns, we sought to determine their importance in the generation of immunity to a recombinant vaccine vector fowlpox virus (fpv). in analyzing the role of type i ifns in immunity t ... | 2010 | 20410285 |
| a safety assessment of a fowlpox-vectored mycoplasma gallisepticum vaccine in chickens. | a recombinant fowlpox virus vaccine expressing key protective mycoplasma gallisepticum antigens could facilitate in the prevention both of fowlpox virus and m. gallisepticum infections. vectormune fp-mg vaccine, a recombinant fowlpox virus expressing both m. gallisepticum 40k and mgc genes, was assessed for its safety in 8-wk-old specific-pathogen-free white leghorn chickens. the vaccine virus was serially passaged 5 times by wing-web inoculation. based on the postinoculation clinical observatio ... | 2010 | 20460677 |
| characterization and efficacy determination of commercially available central american h5n2 avian influenza vaccines for poultry. | a poultry vaccination program was implemented in central america beginning in january 1995 to control both h5n2 low (lpai) and high pathogenicity avian influenza. this study was conducted to identify seed strain composition and the efficacy of 10 commercially available h5 vaccines against challenge with h5n2 lpai viruses isolated from latin america in 2003. the original 1994 vaccine seed virus in commercial inactivated vaccines did not significantly reduce challenge virus shed titers. however, t ... | 2010 | 20470798 |
| high level of protection induced by two fowlpox vector vaccines against a highly pathogenic avian influenza h5n1 challenge in specific-pathogen-free chickens. | the objective of the study was to compare efficacy of two fowlpox (fp) vector vaccines (fp-ai) against h5n1 highly pathogenic avian influenza (hpai): one (vfp89) expressing the native hemagglutinin (ha) gene from h5n8 a/turkey/ ireland/1378/83 and the other (vfp2211) expressing a modified synthetic ha gene from h5n1 a/chicken/indonesia/7/2003. four groups of 20 1-day-old specific-pathogen-free chickens were made: groups 1 and 2 were immunized with 3 log10 tissue-culture infectious dose 50% (tcid ... | 2010 | 20521642 |
| [recombinant cil-18-binding protein as an antagonist to cil-18 enhanced pbmcs secreting ifn-gamma]. | interleukin-18 (il-18) is a proinflammatory cytokines that plays a key role of immune regulation through activating the th 1 cell and nk cell secreting interferon-gamma (ifn-gamma). il-18-binding proteins(il-18bp) secreted in human and mouse can antagonise the activity of il-18. the homologue gene of il-18bp from fowlpox virus genome was predicted and identified of its expressed protein, to use as an antagonist to il-18 guiding disease. | 2010 | 20560354 |
| [induction of potent immune responses by recombinant fowlpox virus with deleted orf73 or orf214]. | to determine if the fowlpox virus (fpv) orf73 or orf214 gene encoding protein has the function of il-18 binding protein, and to assess the role that orf73 or orf214 gene in regulating the immune response. | 2010 | 20560355 |
| vaccine-induced igg2 anti-hiv p24 is associated with control of hiv in patients with a 'high-affinity' fcgammariia genotype. | we have previously shown that vaccination with a recombinant fowlpox virus carrying the genes for hiv gag-pol and interferon-gamma (ifn-gamma) was associated with partial control of hiv replication after antiretroviral therapy (art) was ceased but not with increased anti-hiv t-cell responses. because ifn-gamma enhances igg2 production, and igg2 antibodies to hiv antigens and the 'high-affinity' polymorphism of fcgammariia (the major fc receptor for igg2) have been associated with a favourable ou ... | 2010 | 20634666 |
| canarypox and fowlpox viruses as recombinant vaccine vectors: a biological and immunological comparison. | canarypox and fowlpox viruses represent alternative vaccine vectors due to their natural host-range restriction to avian species. although they cannot replicate in mammals, they correctly express transgenes in human cells and elicit a complete immune response in vaccinated subjects. several studies have evaluated their genomic differences and protective efficacy in preclinical trials, but detailed information is not available for their transgene expression, cytokine modulation and abortive repli ... | 2010 | 20643163 |
| dasatinib alters the metastatic phenotype of b16-ova melanoma in vivo. | the src/abl tyrosine kinase inhibitor dasatinib is an approved chronic myeloid leukemia treatment and is under investigation for solid tumor therapy. members of the src family of kinases (sfks) are involved in the process of metastasis and dasatinib inhibits the migration and invasiveness of human melanoma cell lines in vitro. sfks are also involved in immune function and angiogenesis, which both contribute to as active and passive immunotherapies continue to be investigated in metastatic melano ... | 2010 | 20676039 |
| induction of both cellular and humoral immunity following a rational prime-boost immunization regimen that incorporates recombinant ovine atadenovirus and fowlpox virus. | recombinant fowlpox viruses (rfpv) and ovine atadenoviruses (roadv) are being developed as safe, nonpathogenic, prophylactic and therapeutic vaccine vectors. there is scope, however, to improve the limited immune responses elicited by each of these vaccine vectors. using previously determined and optimized routes of administration and viral doses, we characterized the primary adaptive immune responses elicited by recombinant variants of each virus. we demonstrate the contrasting nature of the re ... | 2010 | 20810681 |
| the development of persistent duck hepatitis b virus infection can be prevented using antiviral therapy combined with dna or recombinant fowlpoxvirus vaccines. | we recently reported the development of a successful post-exposure combination antiviral and "prime-boost" vaccination strategy using the duck hepatitis b virus (dhbv) model of human hepatitis b virus infection. the current study aimed to simplify the vaccination strategy and to test the post-exposure efficacy of combination therapy with the bristol-myers squibb antiviral drug, entecavir (etv) and either a single dose of dhbv dna vaccines on day 0 post-infection (p.i.) or a single dose of recomb ... | 2010 | 20833122 |
| an hiv-1 clade a/e dna prime, recombinant fowlpox virus boost vaccine is safe, but non-immunogenic in a randomized phase i/iia trial in thai volunteers at low risk of hiv infection. | previously demonstrated safe and highly immunogenic in non-human primates, this study assessed dna (phis-hiv-ae) prime, recombinant fowlpox (rfpv-hiv-ae) boost vaccines in humans. | 2010 | 20864808 |
| interaction of poxvirus intracellular mature virion proteins with the tpr domain of kinesin light chain in live infected cells revealed by two-photon-induced fluorescence resonance energy transfer fluorescence lifetime imaging microscopy. | using two-photon-induced fluorescence lifetime imaging microscopy, we corroborate an interaction (previously demonstrated by yeast two-hybrid domain analysis) of full-length vaccinia virus (vacv; an orthopoxvirus) a36 protein with the cellular microtubule motor protein kinesin. quenching of enhanced green fluorescent protein (egfp), fused to the c terminus of vacv a36, by monomeric red fluorescent protein (mdsred), fused to the tetratricopeptide repeat (tpr) domain of kinesin, was observed in li ... | 2010 | 20943972 |
| preliminary molecular characterization of a fowl poxvirus isolate in grenada. | two 1-mo-old local breed chickens, with gross lesions in the skin of the head region suspected to be fowl poxvirus infection, were submitted to the diagnostic laboratory of the school of veterinary medicine, grenada, west indies. cutaneous lesions were collected from these birds for virus isolation, histopathologic diagnosis, and molecular analysis. fowl poxvirus infection was confirmed by virus isolation in chicken embryo and by histopathology. molecular characterization of the fowl poxvirus wa ... | 2010 | 20945792 |
| pathology and microbiology of dermal squamous cell carcinoma in young brown chickens reared on reused litter. | dermal squamous cell carcinoma (dscc) was found in young brown chicken flocks reared on reused litter in japan. dscc was often detected at slaughter from april 2007 to march 2009, especially in june and july 2007. no dscc was observed in the broiler chickens on the farms. twelve 11-wk-old brown chickens with dscc were investigated pathologically and microbiologically. various degrees of crater-like skin lesions were found on the back, waist, neck, legs, abdomen, and wings of the carcasses. the f ... | 2010 | 20945801 |
| construction and immunogenicity of a recombinant fowlpox vaccine coexpressing s1 glycoprotein of infectious bronchitis virus and chicken il-18. | infectious bronchitis virus (ibv) poses a major threat to the chicken industry worldwide. in this study, we developed a recombinant fowlpox virus (rfpv) vaccine expressing the ibv s1 gene and chicken interleukin-18 gene (il-18), rfpv-s1/il18. recombinant plasmid psy-s1/il18 was constructed by cloning chicken il-18 into fowlpox virus transfer plasmid containing s1 gene and transfected into the chicken embryo fibroblasts cell pre-infected with s-fpv-017 to generate rfpv-s1/il18. expression of the ... | 2010 | 20951182 |
| adjuvant activity of chicken interleukin-12 co-administered with infectious bursal disease virus recombinant vp2 antigen in chickens. | a recombinant fowlpox virus (rfpv/vp2) expressing infectious bursal diseases virus (ibdv) vp2 gene has been constructed. after purification and identification of rfpv/vp2, the adjuvant activity of the recombinant chicken il-12 (rchil-12), synthesized by our previous construct of rfpv/chil-12, in rfpv/vp2-expressed rvp2 antigen was assessed in one-week-old specific-pathogen free chickens. the results indicated that rchil-12 alone or rchil-12 plus mineral oil (mo) co-administered with rvp2 antigen ... | 2010 | 21035196 |
| immune responses of mice to prime-boost vaccination with the recombinant dna and fowlpox virus both expressing hiv-2 gag-gp105. | human immunodeficiency viruses 1 and 2 (hiv-1, 2) present a public health problem for which there is neither an effective antiviral therapy nor a preventive vaccine. in this study, the immune responses of mice to prime-boost vaccination with the recombinant dna (rdna) and recombinant fowlpox virus (rfpv) both expressing hiv-2 gag-gp105 chimeric protein, were compared to those elicited by each vector alone. mice primed with the rdna and boosted with the rfpv showed hiv-2-specific antibody levels, ... | 2010 | 21175253 |
| [construction of recombinant fowlpox virus coexpressing ha gene from h5n1 avian influenza virus and chicken interleukin-2 gene and assessment of its protective efficacy]. | the hemagglutinin (ha) gene from h5n1 avian influenza virus and the chicken interleukin 2 (chiil-2) gene were inserted into a expressing vector p12ls to construct a recombinant transferring vector p12lsh5ail2, in which ha gene under the control of the promoter ps was in inverse tandem connection with the chiil-2 gene under the control of the promoter pe/l. the p12lsh5ail2 was then used to transfect the chicken embryo fibroblasts (cef) pre-infected with a wild-type fowlpox virus 282e4 strain, to ... | 2009 | 20077933 |
| impact of a recombinant fowlpox vaccine on the efficacy of adoptive cell therapy with tumor infiltrating lymphocytes in a patient with metastatic melanoma. | a patient with metastatic melanoma who had progressive disease after prior surgical resections, high dose interleukin-2, and anti-cytotoxic t lymphocyte antigen-4 antibody received sequential treatments with autologous tumor infiltrating lymphocytes that recognized the gp100 melanocyte differentiation antigen. although no clinical response was seen when cells were administered alone, an objective clinical response to therapy was seen with tumor infiltrating lymphocytes administered together with ... | 2009 | 19752747 |
| protection of chickens against infectious bronchitis by a recombinant fowlpox virus co-expressing ibv-s1 and chicken ifngamma. | a fowlpox virus expressing the chicken infectious bronchitis virus (ibv) s1 gene of the lx4 strain (rfpv-ibvs1) and a fowlpox virus co-expressing the s1 gene and the chicken type ii interferon gene (rfpv-ibvs1-chifngamma) were constructed. these viruses were assessed for their immunological efficacy on specific-pathogen-free (spf) chickens challenged with a virulent ibv. although the antibody levels in the rfpv-ibvs1-chifngamma-vaccinated group were lower than those in the attenuated live ib vac ... | 2009 | 19786144 |
| comparing human t cell and nk cell responses in viral-based malaria vaccine trials. | vaccination with viral-based vaccines continues to hold promise for the prevention of malaria. whilst antigen-specific t cell responses are considered a major aim of such an approach, a role for induced nk cells as anti-malarial effector cells, or in shaping t cell responses, has received less attention. in this study naïve human volunteers were vaccinated in a prime-boost vaccination regimen comprising recombinant viral vectors fowlpox (fp9) and modified vaccinia ankara (mva) encoding liver-sta ... | 2009 | 19837090 |
| the antitumor and immunoadjuvant effects of ifn-alpha in combination with recombinant poxvirus vaccines. | ifn-alpha is a pleiotropic cytokine possessing immunomodulatory properties that may improve the efficacy of therapeutic cancer vaccines. the aim of this study was to evaluate the effectiveness and compatibility of combining recombinant ifn-alpha with poxvirus vaccines targeting the human carcinoembryonic antigen (cea) in murine models of colorectal and pancreatic adenocarcinomas, where cea is a self-antigen. | 2009 | 19276249 |
| construction and characterization of recombinant fowlpox viruses expressing human papilloma virus e6 and e7 oncoproteins. | human papilloma virus (hpv)-16 is the most prevalent high-risk mucosal genotype and the expression of the e6 and e7 proteins, which can bind to the p53 and p105rb host cell-cycle regulatory proteins, is related to its tumorigenicity. virus-like-particle (vlp)-based immunogens developed recently are successful as prophylactic hpv vaccines. however, given the high number of individuals infected already with hpv and the absence of expression of the l1 structural protein in hpv-infected or hpv-trans ... | 2009 | 19428588 |
| the fowlpox virus bcl-2 homologue, fpv039, interacts with activated bax and a discrete subset of bh3-only proteins to inhibit apoptosis. | apoptosis is a potent immune barrier against viral infection, and many viruses, including poxviruses, encode proteins to overcome this defense. interestingly, the avipoxviruses, which include fowlpox and canarypox virus, are the only poxviruses known to encode proteins with obvious bcl-2 sequence homology. we previously characterized the fowlpox virus protein fpv039 as a bcl-2-like antiapoptotic protein that inhibits apoptosis by interacting with and inactivating the proapoptotic cellular protei ... | 2009 | 19439472 |
| detection and quantitation of fowl adenovirus genome by a real-time pcr assay. | the purpose of the study was to develop a highly sensitive real-time polymerase chain reaction (pcr) assay to detect and quantitate fowl adenovirus (fadv) dna in chicken tissues, using fadv-9 as a model. the assay had a dynamic range of 7 logs and minimum detection limit of 9.4 viral genome copies. it was shown to be highly specific, as tissues from uninfected chickens and other viral genomes, such as those of marek's disease virus, fowlpox virus and infectious laryngotracheitis virus did not pr ... | 2009 | 19442846 |
| squamous cell carcinoma with presence of poxvirus-like inclusions in the foot of a pink-backed pelican (pelecanus rufescens). | squamous cell carcinoma (scc) or avian keratoacanthoma is a neoplastic skin lesion of unknown aetiology that has been well described in birds. some studies have reported that poxviruses may contribute to the onset of scc. here we describe a case of scc on the underside of a pelican's foot. histologically, the tumour consisted of irregular cords of pleomorphic epithelial cells that invaded the adjacent tissues. additionally, keratinized epithelial cells and moderate numbers of keratin pearls were ... | 2009 | 19468940 |
| identification of ongoing human immunodeficiency virus type 1 (hiv-1) replication in residual viremia during recombinant hiv-1 poxvirus immunizations in patients with clinically undetectable viral loads on durable suppressive highly active antiretroviral therapy. | in most human immunodeficiency virus type 1 (hiv-1)-infected individuals who achieve viral loads of <50 copies/ml during highly active antiretroviral therapy (haart), low levels of plasma virus remain detectable for years by ultrasensitive methods. the relative contributions of ongoing virus replication and virus production from hiv-1 reservoirs to persistent low-level viremia during haart remain controversial. hiv-1 vaccination of haart-treated individuals provides a model for examining low-lev ... | 2009 | 19605490 |
| [anti-tumor effects on human laryngeal carcinoma hep-2 of recombinant fowlpox virus expressing chicken anemia virus apoptin gene]. | to investigate the anti-tumor effects and the mechanism of the recombinant fowlpox virus expressing apoptin gene on human laryngeal carcinoma hep-2. | 2009 | 19621600 |
| serologic response against fowl poxvirus and reticuloendotheliosis virus after experimental and natural infections of chickens with fowl poxvirus. | two infection studies in chickens were done to investigate the humoral immune response against fowl poxvirus (fpv) and reticuloendotheliosis virus (rev) after intradermal infection with different passages of a field isolate and with the vaccine strain hp b. the field isolate in a low passage carried the near-full-length rev provirus and induced antibodies to rev, but not to fpv. the vaccine strain carried only remnants of the long terminal repeat and induced antibodies against fpv, but not again ... | 2009 | 19630225 |
| quantitative pcr as a tool to determine the reticuloendotheliosis virus-proviral load of fowl poxvirus. | sequences of the reticuloendotheliosis virus (rev), an avian retrovirus, are integrated into the genome of fowl poxvirus (fpv). we developed and evaluated a quantitative multiplex real-time pcr (multiplex qpcr) assay to determine the rev-proviral load of fpv strains. amplification efficiencies were 98.7% for the amplification of the fpv dna and 88.7% for the amplification of rev-proviral dna. the ratio between fpv dna and rev-proviral dna was calculated from the pcr efficiencies and the threshol ... | 2009 | 19630226 |
| dna binding and cleavage by the fowlpox virus resolvase. | the first steps of poxvirus dna synthesis yield concatemeric arrays of covalently linked genomes. the virus-encoded holliday junction resolvase is required to process concatemers into unit-length genomes for packaging. previous studies of the vaccinia virus resolvase have been problematic due to poor protein solubility. we found that fowlpox virus resolvase was much more tractable. fowlpox resolvase formed complexes with a variety of branched dna substrates, but not linear dna, and had the highe ... | 2009 | 19004818 |
| dasatinib inhibits recombinant viral antigen-specific murine cd4+ and cd8+ t-cell responses and nk-cell cytolytic activity in vitro and in vivo. | dasatinib (bms-354825) is a small molecule src/abl tyrosine kinase inhibitor approved for the treatment of chronic myeloid leukemia and philadelphia chromosome-positive acute lymphoblastic leukemia. members of the src family of kinases are involved in the induction of innate and adaptive immunity. the purpose of this study was to evaluate the inhibitory action of dasatinib on antigen-specific cd8(+) and cd4(+) t-cell function, as well as natural killer (nk) cell cytotoxicity. | 2009 | 19056158 |
| prime-boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in pekin ducks challenged with asian h5n1 hpai. | the efficacy of different vaccination schedules was evaluated in 17-day-old pekin ducks using an experimental inactivated whole virus vaccine based on the h5n9 a/chicken/italy/22a/98 isolate (h5n9-it) and/or a fowlpox recombinant (vfp-h5) expressing a synthetic ha gene from an asian h5n1 isolate (a/chicken/indonesia/7/2003). full protection against clinical signs and shedding was induced by the different vaccination schemes. however, the broadest antibody response and the lowest antibody increas ... | 2009 | 19056442 |
| mhc-restricted cytotoxic t-lymphocyte assay: an improved method based on normal and sv40-immortalized rabbit epidermal target cells. | although several techniques are available to evaluate cell-mediated immunity, numerous difficulties have prevented their use in rabbits. cytotoxic t-lymphocyte (ctl) assays have been used to determine the ex vivo cytolytic activity of cd8+ t-lymphocytes in immunization protocols. however, this assay cannot be performed with rabbit peripheral blood mononuclear cell (pbmc) targets because of their high spontaneous (51)cr release. to overcome this intrinsic difficulty shown by rabbit cells, syngene ... | 2009 | 18955084 |
| recombinant fowlpox virus vector-based vaccine completely protects chickens from h5n1 avian influenza virus. | with the widespread presence of influenza virus h5n1 in poultry and wildlife species, particularly migrating birds, vaccination has become an important control strategy for avian influenza (ai). in this study, the immune efficacy and hemagglutination inhibition (hi) antibody responses induced by a recombinant fowlpox virus (fpv) vector-based rfpv-ha-na vaccine was evaluated in spf and commercial chickens. four-week old spf chickens vaccinated with one dose of vaccine containing 2 x 10(3) plaque ... | 2009 | 19110002 |
| immunogenicity of recombinant modified vaccinia ankara following a single or multi-dose vaccine regimen in rhesus monkeys. | modified vaccinia ankara (mva) is a replication-defective strain of vaccinia virus (vv) that is being investigated in humans as an alternative vaccine against smallpox. understanding the parameters of a mva vaccine regimen that can effectively enhance protective immunity will be important for clinical development. the present studies utilize cohorts of rhesus monkeys immunized with recombinant mva (rmva) or recombinant vv (rvv) vaccine vectors to investigate the magnitude, breadth, and durabilit ... | 2009 | 19168105 |
| [construction of recombinant fowlpox virus coexpressing ha from subtype h5 of avian influenza virus and chicken interleukin-18]. | we developed recombinant fowlpox viruses (rfpv) coexpressing chicken il-18 and h5 aiv ha. | 2008 | 18956750 |
| transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses. | we have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (ibv) in which a full-length cdna corresponding to the ibv genome is inserted into the vaccinia virus genome under the control of a t7 promoter sequence. vaccinia virus as a vector for the full-length ibv cdna has the advantage that modifications can be introduced into the ibv cdna using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. ... | 2008 | 19057872 |
| safety and immunologic response of a viral vaccine to prostate-specific antigen in combination with radiation therapy when metronomic-dose interleukin 2 is used as an adjuvant. | we have previously reported on the safety and immunologic response of a poxvirus-based vaccine encoding prostate-specific antigen (psa) used in combination with radiation therapy in patients with localized prostate cancer. we hypothesized that a "metronomic" dose of interleukin 2 (il-2) as a biological adjuvant would cause less toxicity while maintaining immunologic response. | 2008 | 18698048 |
| recombinant fowlpox virus elicits transient cytotoxic t cell responses due to suboptimal innate recognition and recruitment of t cell help. | recombinant fowlpox viruses (fpvs) have been used in a variety of vaccine strategies; however strong data clearly demonstrating the characteristics of the strength and nature of the resultant immune response elicited by these vectors are lacking. by utilising a recombinant variant of fpv which expresses the nominal antigen chicken ovalbumin (ova), and assessing innate fpv- and ova-specific adaptive immune responses, we show that recombinant fpv induces a rapid type i interferon (ifn) response, m ... | 2008 | 18541346 |
| integration of the reticuloendotheliosis virus envelope gene into the poultry fowlpox virus genome is not universal. | fowlpox virus (fwpv) is found worldwide in poultry and wild birds. fwpv is a natural example of recombination between viruses, as reticuloendotheliosis virus (rev) fragments have been found in all poultry fwpvs and these are implicated in virulence alteration. we aimed to determine the commonality of this phenomenon and analysed fwpvs collected from 128 poultry flocks and birds over the last 10 years. various fragments of both viruses were amplified and sequenced at the fwpv integration site, lo ... | 2008 | 18796713 |
| safety and immunogenicity of recombinant poxvirus hiv-1 vaccines in young adults on highly active antiretroviral therapy. | a trial to evaluate the safety and immunogenicity of recombinant modified vaccinia ankara (mva) and fowlpox (fp) vectors expressing multiple hiv-1 proteins was conducted in twenty hiv-1 infected youth with suppressed viral replication on haart. the mva and fp-based multigene hiv-1 vaccines were safe and well tolerated. increased frequencies of hiv-1 specific cd4+ proliferative responses and cytokine secreting cells were detected following immunization. increased frequencies and breadth of hiv-1 ... | 2008 | 18940219 |
| avipoxvirus in blackcaps (sylvia atricapilla). | from july to september 2005, 1075 wild birds of 37 species were mist-netted at a location in the north-eastern part of the czech republic. the birds were examined for the presence of avipoxvirus lesions. this was demonstrated by electron microscopy in skin lesions in nine of 244 blackcaps (sylvia atricapilla) examined (4% prevalence). blackcaps skin bioptates were processed using the ultrathin section method. in skin bioptates, avipoxviruses were demonstrated in intracytoplasmic inclusions where ... | 2008 | 18202957 |
| antiviral therapy with entecavir combined with post-exposure "prime-boost" vaccination eliminates duck hepatitis b virus-infected hepatocytes and prevents the development of persistent infection. | short-term antiviral therapy with the nucleoside analogue entecavir (etv), given at an early stage of duck hepatitis b virus (dhbv) infection, restricts virus spread and leads to clearance of dhbv-infected hepatocytes in approximately 50% of etv-treated ducks, whereas widespread and persistent dhbv infection develops in 100% of untreated ducks. to increase the treatment response rate, etv treatment was combined in the current study with a post-exposure "prime-boost" vaccination protocol. four gr ... | 2008 | 18206204 |
| immunization with a recombinant fowlpox virus expressing a hepatitis c virus core-e1 polyprotein variant, protects mice and african green monkeys (chlorocebus aethiops sabaeus) against challenge with a surrogate vaccinia virus. | hcv (hepatitis c virus) is a worldwide health problem nowadays. no preventive vaccine is available against this pathogen, and therapeutic treatments currently in use have important drawbacks, including limited efficacy. in the present work a recombinant fowlpox virus, fpcoe1, expressing a truncated hcv core-e1 polyprotein, was generated. fpcoe1 virus generally failed to elicit a humoral immune response against hcv antigens in balb/c mice. by contrast, mice inoculated with fpcoe1 elicited a posit ... | 2008 | 18215116 |
| eimeria tenella: construction of a recombinant fowlpox virus expressing rhomboid gene and its protective efficacy against homologous infection. | a recombinant fowlpox virus (rfpv) expressing the eimeria tenella rhomboid gene was constructed and its protective efficacy against homologous infection in chickens determined. three-day-old-specific pathogen free (spf) chickens were immunized s.c. with 10(2) plaque forming units (pfu), 10(4) pfu, or 10(6) pfu of rfpv-rhomboid, and challenged with 5x10(4) homologous sporulated oocysts 14 days post-immunization (p.i.). the specific antibody response and lymphocyte proliferation were measured 1, 2 ... | 2008 | 18261729 |
| an mhc-restricted cd8+ t-cell response is induced in cattle by foot-and-mouth disease virus (fmdv) infection and also following vaccination with inactivated fmdv. | foot-and-mouth disease virus (fmdv) causes a highly contagious disease of cloven-hooved animals that carries enormous economic consequences. cd8(+) cytotoxic t lymphocytes play an important role in protection and disease outcome in viral infections but, to date, the role of the cd8(+) t-cell immune response to fmdv remains unclear. this study aimed to investigate major histocompatibility complex (mhc) class i-restricted cd8(+) t-cell responses to fmdv in vaccinated and in infected cattle. an in ... | 2008 | 18272757 |
| targeted delivery of murine ifn-gamma using a recombinant fowlpox virus: nk cell recruitment to regional lymph nodes and priming of tumor-specific host immunity. | interferon-gamma (ifn-gamma) is a proinflammatory cytokine that also acts as a potent immunomodulatory agent. in this study, a replication-deficient recombinant avian (fowlpox) virus was engineered to express the murine ifn-gamma gene (rf-muifn-gamma) with the rationale of delivering concentrated levels of the cytokine to a local tissue microenvironment. subcutaneous (s.c.) rf-muifn-gamma administration resulted in ifn-gamma production that (1) was restricted to the tissue microenvironment of th ... | 2008 | 18279103 |
| recombination-mediated genetic engineering of a bacterial artificial chromosome clone of modified vaccinia virus ankara (mva). | the production, manipulation and rescue of a bacterial artificial chromosome clone of vaccinia virus (vac-bac) in order to expedite construction of expression vectors and mutagenesis of the genome has been described (domi & moss, 2002, pnas99 12415-20). the genomic bac clone was 'rescued' back to infectious virus using a fowlpox virus helper to supply transcriptional machinery. we apply here a similar approach to the attenuated strain modified vaccinia virus ankara (mva), now widely used as a sa ... | 2008 | 18286194 |
| [construction of recombinant fowlpox virus expressing e0 gene of classical swine fever virus shimen strain and the animal immunity experiment]. | the csfv e0 gene was amplified from the plasmid pmd18-t-e0 by pcr and cloned into the fpv-p11 and fpv-psy. the identified recombinant dna was transfected into chicken embryo fibroblasts (cef) to package fowlpox virus. e0 gene was confirmed to be integrated into the genome of recombinant fowlpox virus by pcr, and western blot was employed for detection of e0 expression in the chicken embryo fibroblasts infected with recombinant fowlpox virus . the results of elisa showed that systemic immune resp ... | 2008 | 18320824 |
| detection and differentiation of re-emerging fowlpox virus (fwpv) strains carrying integrated reticuloendotheliosis virus (fwpv-rev) by real-time pcr. | current strains of fowlpox virus (fwpv) carrying circulating reticuloendotheliosis virus (fwpv-rev) sequence are becoming more pathogenic to poultry. this is evidenced by the fact that vaccination with current available fwpv vaccines provides limited protection against them. to characterize rev insertions in a collection of both older and more recent field isolates, we developed three different types of adjacent oligoprobes and primer sets from specific genomic locations of fwpv and rev: rev-env ... | 2008 | 17913400 |
| creation of diversity in the animal virus world by inter-species and intra-species recombinations: lessons learned from poultry viruses. | the biological diversity within viruses is one of the largest found in all other forms of nature. many mechanisms contribute to virus diversity and include incorporating genetic material from the host, recombination between viruses belonging to the same or to a different family, and even recombination between viruses normally infecting different hosts. in particular, avian viruses can utilize all three of these mechanisms to generate new viruses. it is well documented that recombinations can occ ... | 2008 | 17922180 |
| immune responses of swine inoculated with a recombinant fowlpox virus co-expressing p12a and 3c of fmdv and swine il-18. | two recombinant fowlpox viruses (rfpv-p1 and rfpv-il18-2ap12a) containing foot-and-mouth disease virus (fmdv) capsid polypeptide, 3c coding regions of o/ny00 were evaluated to determine their abilities to induce humoral and cellular responses in the presence or absence of swine il-18 as genetic adjuvant. the ability to protect swine against homologous virus challenge was examined. all swine were given booster vaccinations at 21 days after the initial inoculation and were challenged 10 days after ... | 2008 | 17706296 |
| protection of chickens from newcastle disease and infectious laryngotracheitis with a recombinant fowlpox virus co-expressing the f, hn genes of newcastle disease virus and gb gene of infectious laryngotracheitis virus. | a recombinant fowlpox virus (rfpv) coexpressing the newcastle disease virus (ndv) fusion and hemagglutinin-neuraminidase genes and infectious laryngothracheitis virus (iltv) glycoprotein b gene was constructed. this virus was then evaluated for its ability to protect specific-pathogen-free (spf) chickens against clinical symptoms and death after challenge by virulent ndv and iltv. spf chickens were grouped and vaccinated with the rfpv and commercial ndv (la sota) and iltv attenuated live vaccine ... | 2008 | 18459306 |
| clinical safety of a viral vector based prostate cancer vaccine strategy. | the primary objective of this phase i study was to evaluate the clinical safety of a vaccine using recombinant vaccinia virus (prime) and recombinant fowlpox virus (boost) in combination with granulocyte-macrophage colony-stimulating factor in patients with prostate cancer. the vaccines contained transgenes for prostate specific antigen, a triad of co-stimulatory molecules and a tumor antigen whose amino acid sequence had been modified to enhance its immunogenicity. secondary end points were imm ... | 2007 | 17707059 |
| [active immunotherapy of prostate cancer with a focus on dendritic cells]. | recurrent or metastatic prostate cancer is generally considered an incurable disease. given the transient benefit from hormone deprivation therapy and limited successes of systemic chemotherapy, alternative treatment modalities are needed both in the situation of psa recurrence and in hormone-refractory disease. prostate cancer cells express several tumor associated antigens which are currently being evaluated as targets for active and specific immunotherapy approaches. dendritic cells (dc) are ... | 2007 | 17896564 |
| improvements to the hemagglutination inhibition test for serological assessment of recombinant fowlpox-h5-avian-influenza vaccination in chickens and its use along with an agar gel immunodiffusion test for differentiating infected from noninfected vaccinated animals. | in general, avian influenza (ai) vaccines protect chickens from morbidity and mortality and reduce, but do not completely prevent, replication of wild ai viruses in the respiratory and intestinal tracts of vaccinated chickens. therefore, surveillance programs based on serological testing must be developed to differentiate vaccinated flocks infected with wild strains of ai virus from noninfected vaccinated flocks in order to evaluate the success of vaccination in a control program and allow conti ... | 2007 | 17992929 |
| concurrent infection in chickens with fowlpox virus and infectious laryngotracheitis virus as detected by immunohistochemistry and a multiplex polymerase chain reaction technique. | a concurrent infection of chickens with infectious laryngotracheitis virus (iltv), a herpesvirus, and fowlpox virus (fwpv), an avipoxvirus, is described. two techniques, an immunohistochemistry (ihc) technique and a multiplex polymerase chain reaction (pcr), were used to examine 11 tissue samples from chickens clinically diagnosed as fwpv-infected, but only ihc was used to examine six tissue-paraffin blocks prepared from turkeys suspected of having fwpv infection. by multiplex pcr, both fwpv and ... | 2007 | 17992932 |
| poxvirus-based vaccine therapy for patients with advanced pancreatic cancer. | an open-label phase 1 study of recombinant prime-boost poxviruses targeting cea and muc-1 in patients with advanced pancreatic cancer was conducted to determine safety, tolerability and obtain preliminary data on immune response and survival. | 2007 | 18039393 |
| influence of ifngamma co-expression on the safety and antiviral efficacy of recombinant fowlpox virus hiv therapeutic vaccines following interruption of antiretroviral therapy. | therapeutic immunization to stimulate host immune responses and control human immunodeficiency virus (hiv-1) replication is being investigated as a supplementary treatment for the management of hiv infection. on completion of an earlier study involving three vaccinations while taking combination antiretroviral therapy (cart), twenty-five subjects with plasma viral load (pvl) <50 copies/ml received a booster vaccination with either placebo (n = 7); fowl pox vaccine (rfpv) expressing hiv-1 gag/pol ... | 2007 | 18340117 |
| comparative efficacy of subtype ae simian-human immunodeficiency virus priming and boosting vaccines in pigtail macaques. | vaccination against aids is hampered by great diversity between human immunodeficiency virus (hiv) strains. heterologous b-subtype-based simian-human immunodeficiency virus (shiv) dna prime and poxvirus boost vaccine regimens can induce partial, t-cell-mediated, protective immunity in macaques. we analyzed a set of dna, recombinant fowlpox viruses (fpv), and vaccinia viruses (vv) expressing subtype ae hiv type 1 (hiv-1) tat, rev, and env proteins and siv gag/pol in 30 pigtail macaques. siv gag-s ... | 2007 | 17050602 |
| full genome sequence and some biological properties of reticuloendotheliosis virus strain apc-566 isolated from endangered attwater's prairie chickens. | reticuloendotheliosis virus (rev) causes runting, high mortality, immunosuppression, and chronic neoplasia associated with t and/or b cell lymphomas in a variety of domestic and wild birds, including attwater's prairie chickens (apc) (tympanuchus cupido attwateri). the complete proviral sequence of a recent rev isolate from apc (rev apc-566) was determined. this virus was isolated from an apc maintained in captivity in a reproduction program intended to avoid its extinction. rev apc-566 was dete ... | 2007 | 17098316 |
| secis elements in the coding regions of selenoprotein transcripts are functional in higher eukaryotes. | expression of selenocysteine (sec)-containing proteins requires the presence of a cis-acting mrna structure, called selenocysteine insertion sequence (secis) element. in bacteria, this structure is located in the coding region immediately downstream of the sec-encoding uga codon, whereas in eukaryotes a completely different secis element has evolved in the 3'-untranslated region. here, we report that secis elements in the coding regions of selenoprotein mrnas support sec insertion in higher euka ... | 2007 | 17169995 |
| prime-boost immunization with dna, recombinant fowlpox virus and vlp(shiv) elicit both neutralizing antibodies and ifngamma-producing t cells against the hiv-envelope protein in mice that control env-bearing tumour cells. | different primings with dna and fowlpox virus (fp) recombinants or fp alone were used in a pre-clinical trial to evaluate and compare immunogenicity and efficacy against hiv/shiv. three immunization regimens were tested in three groups of mice in which the siv gag/pol and hiv-1 env transgenes were separately expressed by dna and fp vectors, followed by vlp(shiv) boosting. all of the protocols were effective in eliciting homologous neutralizing antibodies, although the mice immunized with dna fol ... | 2007 | 17241705 |
| immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing gp5/gp3 of porcine reproductive and respiratory syndrome virus and swine il-18. | two recombinant fowlpox viruses (rfpv-orf5-orf3 and rfpv-il-18-orf5-orf3) containing the orf5/orf3 cdnas of prrsv (strain chang chun) and il-18 of swine were constructed and evaluated for theirs abilities to induce humoral and cellular responses in piglets. in addition, their abilities to protect piglets against homologous virus challenge were examined. all piglets were given booster vaccinations at 21 days after the initial inoculation, and all piglets were challenged at 60 after the initial in ... | 2007 | 17418456 |
| construction and characterization of a recombinant fowlpox virus containing hiv-1 multi-epitope-p24 chimeric gene in mice. | the epidemic of hiv/aids is sweeping across the world. it is of great importance to figure out new ways to curb this disease. epitope-based vaccine is one of these solutions. in this study, a chimeric gene was obtained by combination of a designed hiv-1 multi-epitope gene (meg) and hiv-1 p24 gene. a recombinant plasmid puta2-megp24 was then constructed by inserting megp24 gene into the downstream of the promoter (ati-p7.5x20) of fowlpox virus (fpv) transfer vector puta2. the recombinant plasmid ... | 2007 | 17447028 |
| drug evaluation: therion's rv-psa-tricom + rf-psa-tricom prime-boost prostate cancer vaccine. | therion biologics corp is developing prostvac-vf-tricom, a prime-boost vaccine regimen that consists of a priming injection with a recombinant attenuated vaccinia virus expressing psa and tricom (the company's proprietary triad of costimulatory molecules: icam-1, b7.1 and lymphocyte function-associated antigen-3), and a booster injection with a fowlpox virus expressing the same combination, for the potential treatment of prostate cancer. phase ii clinical trials are underway. | 2007 | 17458173 |
| efficacy of an inactivated and a fowlpox-vectored vaccine in muscovy ducks against an asian h5n1 highly pathogenic avian influenza viral challenge. | the efficacy of an inactivated vaccine containing the eurasian isolate a/chicken/italy/22a/98 h5n9 (h5n9-it) was compared with that of the fowlpox-vectored trovactm-aiv h5 (rfp-aiv-h5) vaccine against an h5n1 highly pathogenic avian influenza challenge. five-week-old muscovy ducks were vaccinated with either h5n9-it (0.5 ml) or rfp-aiv-h5 (5 log10 50% tissue culture infectious dose (tcid50)/dose), followed by a boost at 7 wk of age with the same vaccine (1.0 ml of h5n9-it or 5 log10 tcid50/dose ... | 2007 | 17494576 |
| the dog that did not bark: malaria vaccines without antibodies. | to date, the only pre-blood stage vaccine to confer protection against malaria in field trials elicits both antigen-specific antibody and t-cell responses. recent clinical trials of new heterologous prime-boost malaria vaccine regimens using dna, fowlpox or mva, have chiefly elicited t-cell responses that have promisingly reduced hepatic merozoites in challenge trials, but failed to protect in field trials. these encouraging results suggest further augmentation of t-cell responses to pre-blood s ... | 2007 | 17512252 |
| heterologous prime/boost immunization of rhesus monkeys by using diverse poxvirus vectors. | as the diversity of potential immunogens increases within certain classes of vectors, the possibility has arisen of employing heterologous prime/boost immunizations using diverse members of the same family of vectors. the present study was initiated to explore the use of divergent pox vectors in a prime/boost regimen to elicit high-frequency cellular immune responses to human immunodeficiency virus type 1 envelope and simian immunodeficiency virus gag in rhesus monkeys. we demonstrated that monk ... | 2007 | 17553898 |
| recovery of genetically defined murine norovirus in tissue culture by using a fowlpox virus expressing t7 rna polymerase. | despite the significant disease burden caused by human norovirus infection, an efficient tissue-culture system for these viruses remains elusive. murine norovirus (mnv) is an ideal surrogate for the study of norovirus biology, as the virus replicates efficiently in tissue culture and a low-cost animal model is readily available. in this report, a reverse-genetics system for mnv is described, using a fowlpox virus (fwpv) recombinant expressing t7 rna polymerase to recover genetically defined mnv ... | 2007 | 17622609 |
| therapeutic vaccination against murine lymphoma by intratumoral injection of recombinant fowlpox virus encoding cd40 ligand. | the interaction between cd40 ligand (cd40l, cd154) and its receptor cd40 on antigen-presenting cells is essential for the initiation of cell-mediated and humoral immune responses. malignant b cells also express cd40 and respond to cd40l by enhancing expression of costimulatory molecules. in this study, we investigated the therapeutic antitumor effect of intratumoral administration of recombinant fowlpox virus encoding murine cd40l (rf-mcd40l) in a murine b-cell lymphoma model. balb/c mice with e ... | 2007 | 17638917 |
| [self-excising reporter gene in recombinant fowlpox virus expressing h5 hemagglutinin gene of influenza a virus using cre-loxp systerm]. | hemagglutinin gene of subtype h5 avian influenza virus was amplified by polymerase chain reaction to construct expression cassette containing fpv early, late promoter and sv40 polya tail. then delivery vector was constructed by subcloning hemagglutinin gene of subtype h5 and gfp gene into fowlpox virus recombinant arm. the delivery vector and lipid were transfected into cef cells preinfected with fpv 282e4 strain virus. recombinant fowlpox virus expressing the green fluorescence protein and hema ... | 2007 | 17672322 |
| fowlpox virus encodes a bcl-2 homologue that protects cells from apoptotic death through interaction with the proapoptotic protein bak. | poxviruses are renowned for encoding numerous immunomodulatory proteins capable of undermining potent immune defenses. one effective barrier against infection is apoptosis, a process controlled at the mitochondria by pro- and antiapoptotic members of the highly conserved bcl-2 family of proteins. although poxviruses are known to encode an array of effective inhibitors of apoptosis, members of the avipoxvirus genus, which includes fowlpox virus, encode proteins with bcl-2 homology. here, we show ... | 2007 | 17686864 |
| [construction and screening of recombinant fowlpox virus expressing eimeria tenella f2 hybrid strain so7 gene]. | to construct the recombinant fowlpox virus expressing eimeria tenella f2 hybrid strain so7 gene. | 2006 | 17361816 |
| development of a method for bacteria and virus recovery from heating, ventilation, and air conditioning (hvac) filters. | the aim of the work presented here is to study the effectiveness of building air handling units (ahus) in serving as high volume sampling devices for airborne bacteria and viruses. an hvac test facility constructed according to ashrae standard 52.2-1999 was used for the controlled loading of hvac filter media with aerosolized bacteria and virus. nonpathogenic bacillus subtilis var. niger was chosen as a surrogate for bacillus anthracis. three animal viruses; transmissible gastroenteritis virus ( ... | 2006 | 17240906 |
| retention of 1.2 kbp of 'novel' genomic sequence in two european field isolates and some vaccine strains of fowlpox virus extends open reading frame fpv241. | the emergence of variant fowlpox viruses (fwpvs) and increasing field use of recombinants against avian influenza h5n1 emphasize the need to monitor vaccines and to distinguish them from field strains. five commercial vaccines, two laboratory viruses and two european field isolates were characterized by pcr and sequencing at 18 loci differing between attenuated fp9 and its pathogenic progenitor. pcr failed to discriminate between the viruses and sequence determination revealed no significant dif ... | 2006 | 17098969 |
| development and use of fowlpox vectored vaccines for avian influenza. | the avian influenza (ai) vaccine designated trovac-aiv h5 (trovac-h5) contains a live recombinant fowlpox rec. (fp) recombinant (recfp), expressing the hemagglutinin (ha) gene of an ai h5 subtype isolate. this recombinant vaccine was granted a license in the united states for emergency use in 1998 and full registration in mexico, guatemala, and el salvador where over 2 billion doses have been administered. one injection of trovac-h5 protects chickens against ai-induced mortality and morbidity fo ... | 2006 | 17135511 |