Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| normal values for heart electrophysiology parameters of healthy swine determined on electrophysiology study. | swine are a well-recognized animal model for human cardiovascular diseases. despite the widespread use of porcine model in experimental electrophysiology, still no reference values for intracardiac electrical activity and conduction parameters determined during an invasive electrophysiology study (eps) have been developed in this species thus far. | 2017 | 28028980 |
| impact of synthetic elements on aortic root haemodynamics: computed fluid dynamics of aortic root reconstruction and valve reimplantation. | the aim was to evaluate the impact of the aortic valve reimplantation (david) and of the aortic root (aor) remodelling (yacoub) on the aor haemodynamics. | 2017 | 28013289 |
| anemia tolerance during normo-, hypo-, and hypervolemia. | restrictive intraoperative fluid management has been demonstrated to improve outcome of visceral and lung surgery in several studies. however, subsequent hypovolemia (hov) may be accompanied by a decrease of anemia tolerance, resulting in increased transfusion needs. we therefore investigated the effect of volume status on anemia tolerance. | 2017 | 27990642 |
| safety and efficacy of new biodegradable polymer-based sirolimus-eluting stents in a preclinical model. | new drug-eluting stents (des) designed to overcome the limitations of existing devices should initially be tested in preclinical studies. our objective was to analyze the safety and efficacy of new biodegradable polymer-based des compared with bare-metal stents (bms) and commercially available des in a model of normal porcine coronary arteries. | 2017 | 28647315 |
| annotation of the domestic pig genome by quantitative proteogenomics. | the pig is one of the earliest domesticated animals in the history of human civilization and represents one of the most important livestock animals. the recent sequencing of the sus scrofa genome was a major step toward the comprehensive understanding of porcine biology, evolution, and its utility as a promising large animal model for biomedical and xenotransplantation research. however, the functional and structural annotation of the sus scrofa genome is far from complete. here, we present mass ... | 2017 | 28625053 |
| protein requirements of collared peccary (pecari tajacu). | a nitrogen (n) balance digestion trial was conducted to determine the protein requirement of collared peccaries (pecari tajacu). in a 4 × 4 latin square design, four captive adult male peccaries were fed four isoenergy diets containing four different levels of n (11.7, 16.3, 22.8, and 26.7 g n/kg of dry matter-dm). after 15 days of adaptation, a total collection of feces and urine was carried out for five consecutive days. regression analyses between n intake and n in feces and urine allowed to ... | 2017 | 28623602 |
| adrenergic regulation of cytoplasmic structures related to secretory processes in pig pinealocytes-an ultrastructural, quantitative study. | two structures, considered as secretory in nature, are present in the pinealocytes in of the domestic pig show the presence of two structures, which are considered as secretory in nature - the dense core vesicles (dcv) and the membrane bounded (dense) bodies (mbb). the latter are extremely numerous in pig pinealocytes (they occupy 6-20% of the cytoplasm), and the number of mbb changes under different physiological and experimental conditions. norepinephrine is the main neurotransmitter that regu ... | 2017 | 28622599 |
| epidemiological studies on trichinellosis in pigs (sus scofa) in india. | the present study was conducted to determine the prevalence of trichinellosis in domestic pigs (sus scrofa)in india. a total of 319 tissue and 279 serum samples were collected from 279 slaughter pigs. the samples were collected from small slaughter shops located in assam, chandigarh (union territory), punjab and uttarakhand states of the country. the presence oftrichinellaspp. antibodies in serum was determined using a commercially available enzyme-linked immunosorbent assay (elisa) kit and tiss ... | 2017 | 28615865 |
| genome-wide dynamics of nascent noncoding rna transcription in porcine heart after myocardial infarction. | microarrays and rna sequencing are widely used to profile transcriptome remodeling during myocardial ischemia. however, the steady-state rna analysis lacks in sensitivity to detect all noncoding rna species and does not provide separation between transcriptional and post-transcriptional regulations. here, we provide the first comprehensive analysis of nascent rna profiles of mrnas, primary micro-rnas, long noncoding rnas, and enhancer rnas in a large animal model of acute infarction. | 2017 | 28611032 |
| repeated subretinal surgery and removal of subretinal decalin is well tolerated - evidence from a porcine model. | subretinal perfluorocarbon liquid (pfcl) is a serious complication that can occur after retinal detachment repair. it is possible to remove the pfcl surgically, but retinal damage related to the procedure is unknown. also, increasing interest in subretinal treatment makes it relevant to examine the functional and morphological consequences of repeated subretinal manipulation. we hypothesized that pfcl in a porcine model can be injected in the subretinal space and removed with minimal effect on r ... | 2017 | 28608271 |
| periosteal elevation induces supracortical peri-implant bone formation. | the aim of the study was to evaluate the possibility of supracortical peri-implant bone formation after periosteal elevation. | 2017 | 28606438 |
| reliability of transcardiopulmonary thermodilution cardiac output measurement in experimental aortic valve insufficiency. | monitoring cardiac output (co) is important to optimize hemodynamic function in critically ill patients. the prevalence of aortic valve insufficiency (ai) is rising in the aging population. however, reliability of co monitoring techniques in ai is unknown. the aim of this study was to investigate the impact of ai on accuracy, precision, and trending ability of transcardiopulmonary thermodilution-derived cotcptd in comparison with pulmonary artery catheter thermodilution copac. | 2017 | 29049339 |
| population admixture in chinese and european sus scrofa. | relationships between different populations were investigated using porcine 60 k data from 1,135 domestic pigs and wild boars across europe and china. the results indicate that most european breeds have been introgressed with chinese ancestry, but the extent of introgression varies considerably among breeds. moreover, the main source of this introgression is pigs from south china, closely related to bamaxiang and dongshan pigs. contributions from east and central chinese pig breeds are also dete ... | 2017 | 29030577 |
| simultaneous profiles of sulfonated androgens, sulfonated estrogens and sulfonated progestogens in postpubertal boars (sus scrofa domestica) measured by lc-ms/ms. | sulfonated steroids (s-st) have been usually regarded as inactive metabolites but are progressively considered as precursors for the intra-tissue formation of bioactive steroids. moreover, independent effects without preceding removal of the sulfate group have been observed. we use the porcine testicular-epididymal compartment as a model to investigate the still largely unknown s-st physiology as the boar exhibits an intriguingly broad s-st spectrum predominantly originating from the testis. the ... | 2017 | 29030154 |
| biocompatibility and osseointegration of titanium implants with a silver-doped polysiloxane coating: an in vivo pig model. | to test the antimicrobial properties, surface topography, reaction of surrounding tissue (biocompatibility), and osseointegration of ultrathin implant surfaces containing polysiloxane and nanoscaled silver particles. | 2017 | 29028852 |
| why is african swine fever still present in sardinia? | african swine fever (asf) is an infectious disease of swine that has been present in sardinia since 1978. soon after introduction of the disease, several control and eradication programmes were established with limited success. some researchers attributed the persistence of the disease in central and eastern areas to certain socio-economic factors, the existence of some local and traditional farming practices (i.e., unregistered free-ranging pigs known as brado animals) and the high density of w ... | 2017 | 29027378 |
| characterization of the african swine fever virus decapping enzyme during infection. | african swine fever virus (asfv) infection is characterized by a progressive decrease in cellular protein synthesis with a concomitant increase in viral protein synthesis, though the mechanism by which the virus achieves this is still unknown. decrease of cellular mrna is observed during asfv infection, suggesting that inhibition of cellular proteins is due to an active mrna degradation process. asfv carries a gene (ba71v d250r/malawi g5r) that encodes a decapping protein (asfv-dp) that has a nu ... | 2017 | 29021398 |
| a preclinical evaluation comparing the performance of a novel 19-g flexible needle to a commercially available 22-g ebus-tbna sampling needle. | needle samples may not provide sufficient diagnostic material for the assessment of mediastinal lymph nodes. | 2017 | 29020679 |
| signatures of selection and interspecies introgression in the genome of chinese domestic pigs. | chinese domestic pigs have experienced strong artificial selection for thousands of years. however, the molecular mechanisms underlying the selection-causing phenotypic changes in chinese domestic pigs are still largely unknown. here we used whole-genome resequencing data of 54 pigs from 9 chinese diverse breeds and 16 wild boars from 7 localities across china to identify genes that show evidence of positive selection in the process of domestication. a total of 14 candidate domestication regions ... | 2017 | 29016799 |
| improved method for the establishment of an in vitro blood-brain barrier model based on porcine brain endothelial cells. | the aim of this protocol presents an optimized procedure for the purification and cultivation of pbecs and to establish in vitro blood-brain barrier (bbb) models based on pbecs in mono-culture (mc), mc with astrocyte-conditioned medium (acm), and non-contact co-culture (ncc) with astrocytes of porcine or rat origin. pbecs were isolated and cultured from fragments of capillaries from the brain cortices of domestic pigs 5-6 months old. these fragments were purified by careful removal of meninges, ... | 2017 | 28994773 |
| ex vivo treatment with inhaled n-acetylcysteine in porcine lung transplantation. | we have shown the beneficial effects of n-acetylcysteine (nac) on posttransplant lung function, when both donor and recipient were pretreated intravenously. however, systemic treatment of multiorgan donors may not be clinically relevant. thus, we hypothesized that ex vivo treatment of donors with nebulized nac would be adequate to prevent from ischemia-reperfusion injury after lung transplantation. | 2017 | 28985871 |
| molecular study of porcine circovirus type 2 in wild boars and domestic pigs in uruguay from 2010 to 2014: predominance of recombinant circulating strains. | porcine circovirus type 2 (pcv2) is a worldwide distributed pathogen and one of the most economically relevant swine infections. four genotypes have been recognized and it is well known that pcv2a, pcv2b and pcv2d have a global distribution. however, the information about recombinant strains circulation and their influence in driving pcv2 evolution is a poorly studied area. in uruguay, pcv2 associated symptoms began to be frequently observed in pigs from different farms since 2010. the main purp ... | 2017 | 28964894 |
| complete genome sequences and phylogenetic analysis of encephalomyocarditis virus strains isolated from pigs and rats origin. | in order to evaluate the genetic variability of encephalomyocarditis virus (emcv), the whole genomes of six emcv field isolates originating from pigs and rats origin in different regions of central china, were phylogenetically and comparatively analyzed. phylogenetic analysis of whole genome sequences, open reading frame (orf), capsid coding region (ccr) and vp3/vp1 using neighbor-joining analysis revealed that these isolates belonged to lineage 1. nucleotide sequences of six isolates showed mor ... | 2017 | 28964856 |
| detection of toxoplasma gondii in naturally infected domestic pigs in northern serbia. | insufficiently cooked pork is considered as an important source of human infection with toxoplasma gondii. the aim of our study was to investigate the presence of t. gondii in pigs intended for human consumption from northern serbia. blood and diaphragm samples were collected from 182 naturally infected market-weight pigs, originating from both commercial farms and smallholdings. sera were examined using modified agglutination test (mat), and diaphragms from seropositive, as well as from some ma ... | 2017 | 28956155 |
| interventional magnetic resonance imaging guided carotid embolectomy using a novel resonant marker catheter: demonstration of preclinical feasibility. | to assess the visualization and efficacy of a wireless resonant circuit (wrc) catheter system for carotid artery occlusion and embolectomy under real-time mri guidance in vivo, and to compare mr imaging modality with x-ray for analysis of qualitative physiological measures of blood flow at baseline and after embolectomy. the wrc catheter system was constructed using a mr compatible peek fiber braided catheter (penumbra, inc, alameda, ca) with a single insulated longitudinal copper loop soldered ... | 2017 | 28948399 |
| single step genome-wide association studies based on genotyping by sequence data reveals novel loci for the litter traits of domestic pigs. | in this study, data genotyping by sequence (gbs) was used to perform single step gwas (ssgwas) to identify snps associated with the litter traits in domestic pigs and search for candidate genes in the region of significant snps. after quality control, 167,355 high-quality snps from 532 pigs were obtained. phenotypic traits on 2112 gilt litters from 532 pigs were recorded including total number born (tnb), number born alive (nba), and litter weight born alive (lwb). a single-step genomic blup app ... | 2017 | 28943389 |
| multiplex real-time polymerase chain reaction for the differential detection of porcine circovirus 2 and 3. | a multiplex quantitative real-time polymerase chain reaction (mqpcr) assay was developed for the rapid and differential detection of porcine circovirus 2 (pcv2) and pcv3. each of the capsid genes of pcv2 and pcv3 were amplified using specific primers and probe sets, while no other porcine pathogen genes were detected. limit of detection of the assay was below 50 copies of the target genes of pcv2 and pcv3, and was comparable to that of previously described methods the assay showed high repeatabi ... | 2017 | 28941615 |
| genome-wide snp data unveils the globalization of domesticated pigs. | pigs were domesticated independently in eastern and western eurasia early during the agricultural revolution, and have since been transported and traded across the globe. here, we present a worldwide survey on 60k genome-wide single nucleotide polymorphism (snp) data for 2093 pigs, including 1839 domestic pigs representing 122 local and commercial breeds, 215 wild boars, and 39 out-group suids, from asia, europe, america, oceania and africa. the aim of this study was to infer global patterns in ... | 2017 | 28934946 |
| diagnostic specificity of the african swine fever virus antibody detection enzyme-linked immunosorbent assay in feral and domestic pigs in the united states. | african swine fever (asf) is a highly contagious haemorrhagic disease of pigs that has the potential to cause mortality nearing 100% in naïve animals. while an outbreak of asf in the united states' pig population (domestic and feral) has never been reported, an introduction of the disease has the potential to cause devastation to the pork industry and food security. during the recovery phase of an outbreak, an antibody detection diagnostic assay would be required to prove freedom of disease with ... | 2017 | 28921873 |
| vocal correlates of emotional reactivity within and across contexts in domestic pigs (sus scrofa). | vocalizations have long been recognized to encode information about an individual's emotional state and, as such, have contributed to the study of emotions in animals. however, the potential of vocalizations to also encode information about an individual's emotional reactivity has received much less attention. in this study, we aimed to test whether the vocalizations of domestic pigs contain correlates of emotional reactivity that are consistent between different contexts. we recorded vocalizati ... | 2017 | 28912089 |
| persistence of mycoplasma hyopneumoniae sequence types in spite of a control program for enzootic pneumonia in pigs. | enzootic pneumonia (ep) in pigs caused by mycoplasma (m.) hyopneumoniae has successfully been combatted in switzerland. a control program was fully implemented in 2004 which is based on total depopulation strategies of affected fattening farms as well as partial depopulation on breeding farms. thereby, the number of cases has dropped drastically from more than 200 in 2003 to two cases in 2013. currently monitoring is done based on clinical observation and subsequent diagnostic of coughing pigs. ... | 2017 | 28903876 |
| evolution and genome specialization of brucella suis biovar 2 iberian lineages. | swine brucellosis caused by b. suis biovar 2 is an emergent disease in domestic pigs in europe. the emergence of this pathogen has been linked to the increase of extensive pig farms and the high density of infected wild boars (sus scrofa). in portugal and spain, the majority of strains share specific molecular characteristics, which allowed establishing an iberian clonal lineage. however, several strains isolated from wild boars in the north-east region of spain are similar to strains isolated i ... | 2017 | 28899413 |
| immortalization and characterization of porcine macrophages that had been transduced with lentiviral vectors encoding the sv40 large t antigen and porcine telomerase reverse transcriptase. | the domestic pig is an important agricultural animal, and thus, infectious diseases that affect pigs can cause severe economic losses in the global swine industry. various porcine pathogens target macrophages, which are classical innate immune cells. although macrophages basically protect the host from pathogens, they also seem to contribute to infectious processes. therefore, cultured macrophages can be used to developin vitromodels for studying not only genes associated with porcine innate imm ... | 2017 | 28871285 |
| genomic characteristics ofbifidobacterium thermacidophilumpig isolates and wild boar isolates reveal the unique presence of a putative mobile genetic element withtetwfor pig farm isolates. | genomic analysis was performed on seven strains ofbifidobacterium thermacidophilum, asus-associatedbifidobacterium. three strains from the feces of domestic pigs (sus scrofa domesticus) and four strains from the rectal feces of free-range japanese wild boars (s. s. scrofa) were compared. the phylogenetic position of these isolates suggested by genomic analyses were not concordant with that suggested by 16s rrna sequence. there was biased distribution of genes for virulence, phage, metabolism of ... | 2017 | 28861055 |
| corrigendum. | sayler ka, bigelow t, koster lg, swenson s, bounds c, hernández f, wisely sm. development of a rapid, simple, and specific real-time pcr assay for detection of pseudorabies viral dna in domestic swine herds. j vet diagn invest 2017;29:522-528. (original doi:10.1177/1040638717706593). in the article titled "development of a rapid, simple, and specific real-time pcr assay for detection of pseudorabies viral dna in domestic swine herds" by katherine a. sayler et al., the acknowledgements section, s ... | 2017 | 28859602 |
| synthesis, radiofluorination, and preliminary evaluation of the potential 5-ht2areceptor agonists [18f]cimbi-92 and [18f]cimbi-150. | an agonist pet tracer is of key interest for the imaging of the 5-ht2areceptor, as exemplified by the previously reported success of [11c]cimbi-36. fluorine-18 holds several advantages over carbon-11, making it the radionuclide of choice for clinical purposes. in this respect, an18f-labelled agonist 5-ht2areceptor (5-ht2ar) tracer is highly sought after. herein, we report a 2-step, 1-pot labelling methodology of 2 tracer candidates. both ligands display high in vitro affinities for the 5-ht2ar. ... | 2017 | 28856700 |
| the impact of alteplase on pulmonary graft function in donation after circulatory death - an experimental study. | lung transplantation is hampered by the lack of organs resulting in deaths on the waiting list. the usage of donation after circulatory death (dcd) lungs would dramatically increase donor availability. the most optimal organ preservation method, and the need for antithrombotic and fibrinolytic treatment to prevent thrombosis in the donor lungs is currently on debate. the present study investigated, in a simulated clinical dcd situation, whether the addition of alteplase in the flush-perfusion so ... | 2017 | 28839932 |
| the epidemiology of african swine fever in "nonendemic" regions of zambia (1989-2015): implications for disease prevention and control. | african swine fever (asf) is a highly contagious and deadly viral hemorrhagic disease of swine. in zambia, asf was first reported in 1912 in eastern province and is currently believed to be endemic in that province only. strict quarantine measures implemented at the luangwa river bridge, the only surface outlet from eastern province, appeared to be successful in restricting the disease. however, in 1989, an outbreak occurred for the first time outside the endemic province. sporadic outbreaks hav ... | 2017 | 28832525 |
| multiple stent delivery system multi-loc, a new technology for spot-stenting of the femoropopliteal artery - proof of concept study in a preclinical large animal model. | a new stent system was studied in a porcine model to evaluate its feasibility for spot-stenting of the femoropopliteal artery. | 2017 | 28825354 |
| convergent and divergent genetic changes in the genome of chinese and european pigs. | since 10,000 bc, continuous human selection has led to intense genetic and phenotypic changes in pig (sus scrofa) domestication. through whole genome analysis of 257 individuals, we demonstrated artificial unidirectional and bidirectional selection as the primary force to shape the convergent and divergent changes between chinese domestic pigs (chd) and european domestic pigs (eud). we identified 31 genes in unidirectional selection regions that might be related to fundamental domestication requ ... | 2017 | 28819228 |
| effect of ha330 resin-directed hemoadsorption on a porcine acute respiratory distress syndrome model. | blood purification is an emerging approach to dampening the cytokine storm. this study aims to assess the efficacy of ha330 resin-directed hemoadsorption (ha) on endotoxin-induced porcine acute respiratory distress syndrome (ards) model. | 2017 | 28808944 |
| origin and dispersal of early domestic pigs in northern china. | it is widely accepted that modern pigs were domesticated independently at least twice, and chinese native pigs are deemed as direct descendants of the first domesticated pigs in the corresponding domestication centers. by analyzing mitochondrial dna sequences of an extensive sample set spanning 10,000 years, we find that the earliest pigs from the middle yellow river region already carried the maternal lineages that are dominant in both younger archaeological populations and modern chinese pigs. ... | 2017 | 28798357 |
| quantitative approach for the risk assessment of african swine fever and classical swine fever introduction into the united states through legal imports of pigs and swine products. | the us livestock safety strongly depends on its capacity to prevent the introduction of transboundary animal diseases (tads). therefore, accurate and updated information on the location and origin of those potential tads risks is essential, so preventive measures as market restrictions can be put on place. the objective of the present study was to evaluate the current risk of african swine fever (asf) and classical swine fever (csf) introduction into the us through the legal importations of live ... | 2017 | 28797058 |
| a test for paedomorphism in domestic pig cranial morphology. | domestic animals are often described as paedomorphic, meaning that they retain juvenile characteristics into adulthood. through a three-dimensional landmark-based geometric morphometric analysis of cranial morphology at three growth stages, we demonstrate that wild boar (n= 138) and domestic pigs (n= 106) (sus scrofa) follow distinct ontogenetic trajectories. with the exception of the size ratio between facial and neurocranial regions, paedomorphism does not appear to be the primary pattern desc ... | 2017 | 28794276 |
| preclinical efficacy and safety of kcnh2-g628s gene therapy for postoperative atrial fibrillation. | postoperative atrial fibrillation (poaf) is the most common complication occurring after cardiac surgery. multiple studies have shown significantly increased risks of stroke, myocardial infarction, and death associated with poaf. current prophylaxis strategies are inadequate to eliminate this problem. we examined the preclinical efficacy and safety of kcnh2-g628s gene transfer to prevent poaf. | 2017 | 28676183 |
| immunoregulatory effects of multipotent adult progenitor cells in a porcine ex vivo lung perfusion model. | primary graft dysfunction (pgd) is considered to be the end result of an inflammatory response targeting the new lung allograft after transplant. previous research has indicated that mapc cell therapy might attenuate this injury by its paracrine effects on the pro-/anti-inflammatory balance. this study aims to investigate the immunoregulatory capacities of mapc cells in pgd when administered in the airways. | 2017 | 28676074 |
| a high sucrose and high fat diet induced the development of insulin resistance in the skeletal muscle of bama miniature pigs through the akt/glut4 pathway. | a high sucrose and high fat (hshf) diet induces insulin resistance (ir) and increased susceptibility to type 2 diabetes mellitus (t2dm), but the underlying mechanisms are poorly characterized. this study aimed to investigate the molecular mechanisms by which the hshf diet impairs insulin sensitivity in bama miniature pigs (sus scrofa domesticus). twelve bama miniature pigs were randomly assigned to the control diet (cd) group (n=6) or the hshf group (n=6) for 6 months. biochemical parameters wer ... | 2017 | 28674285 |
| physiological mechanisms through which heat stress compromises reproduction in pigs. | seasonal variations in environmental temperatures impose added stress on domestic species bred for economically important production traits. these heat-mediated stressors vary on a seasonal, daily, or spatial scale, and negatively impact behavior and reduce feed intake and growth rate, which inevitably lead to reduced herd productivity. the seasonal infertility observed in domestic swine is primarily characterized by depressed reproductive performance, which manifests as delayed puberty onset, r ... | 2017 | 28667793 |
| endoluminal nd:yag laser application in ex vivo biliary porcine tissue. | adequate biliary drainage with endoscopic or percutaneous placement of self-expandable metal stents represents the goal of palliation in patients with inoperable malignant obstruction of the biliary tree. as an adjunct to stenting, various tissue ablation treatments have been proposed with conflicting results. the aim of this study was to test the effect on biliary tissue of a new ablation technique based on nd:yag laser light delivery. the study was conducted on ex vivo specimens of 18 healthy ... | 2017 | 28667507 |
| questionnaire-based assessment of wild boar/domestic pig interactions and implications for disease risk management in corsica. | wild boars and domestic pigs belong to the same species (sus scrofa). when sympatric populations of wild boars, feral pigs, and domestic pigs share the same environment, interactions between domestic and wild suids (idws) are suspected to facilitate the spread and maintenance of several pig pathogens which can impact on public health and pig production. however, information on the nature and factors facilitating those idws are rarely described in the literature. in order to understand the occurr ... | 2017 | 29250528 |
| a nationwide serosurvey of hepatitis e virus antibodies in the general population of portugal. | evidence has shown that hepatitis e virus (hev) genotype 3 is autochthonous in industrialized countries due to zoonotic transmission through direct contact or consumption of raw or undercooked meat from domestic swine or wild boar. as there is lack of data on seroprevalence of hev in the general portuguese population, a wide survey was conducted as part of the hepecontrol project (60dt2), under eea grants funding. | 2017 | 29237007 |
| comparison of continuous compression with regular ventilations versus 30:2 compressions-ventilations strategy during mechanical cardiopulmonary resuscitation in a porcine model of cardiac arrest. | a compression-ventilation (c:v) ratio of 30:2 is recommended for adult cardiopulmonary resuscitation (cpr) by the current american heart association (aha) guidelines. however, continuous chest compression (ccc) is an alternative strategy for cpr that minimizes interruption especially when an advanced airway exists. in this study, we investigated the effects of 30:2 mechanical cpr when compared with ccc in combination with regular ventilation in a porcine model. | 2017 | 29221300 |
| hepatitis e seroprevalence and genotyping in a cohort of wild boars in southern germany and eastern alsace. | in the last few years it has been realized that the hepatitis e virus (hev) is endemic in most industrialized countries and that it is a zoonotic disease. potential reservoirs for hev have been identified to be wild boars and deers, but hev has also been found in domestic pigs and other animals. due to the probable spread of the virus via contaminated food or contact to infected animals, hev antibodies are present in more than 16% of the german adult population and rates are increasing with age. ... | 2017 | 29214558 |
| effects of intramuscularly administered enrofloxacin on the susceptibility of commensal intestinal escherichia coli in pigs (sus scrofa domestica). | in the european union, various fluoroquinolones are authorised for the treatment of food producing animals. each administration poses an increased risk of development and spread of antimicrobial resistance. the aim of this study was to investigate the impact of parenteral administration of enrofloxacin on the prevalence of enrofloxacin and ciprofloxacin susceptibilities in the commensal intestinal e. coli population. | 2017 | 29202759 |
| behavioural lateralization in domestic pigs (sus scrofa)-variations between motor functions and individuals. | motor lateralization is hypothesized to depend on the complexity of the motor function, but it might at the same time reflect hemispheric dominance within an individual across motor functions. we investigated possible motor lateralization patterns in four motor functions of different complexity (snout use in a manipulative task, foot use in two-stepping tasks and tail curling) in the domestic pig, a tetrapod species relevant as farm animal but also as a model in human neuroscience. a significant ... | 2017 | 29191074 |
| magnetic resonance elastography can monitor changes in medullary stiffness in response to treatment in the swine ischemic kidney. | low-energy shockwave (sw) therapy attenuates damage in the stenotic kidney (stk) caused by atherosclerotic renal artery stenosis (aras). we hypothesized that magnetic resonance elastography (mre) would detect attenuation of fibrosis following sw in unilateral aras kidneys. | 2017 | 29289980 |
| mitochondrial targeted peptides preserve mitochondrial organization and decrease reversible myocardial changes in early swine metabolic syndrome. | the mechanisms responsible for cardiac damage in the early stages of metabolic syndrome (mets) remain unknown. mitochondria are intimately associated with cellular myofibrils, with the cytoskeleton functioning as a linkage coordinator, and closely associated to the calcium release sites of the sarcoplasmic reticulum (sr). objective: we hypothesized that early mets is characterized by mitochondria-related myocardial damage, associated with altered cytoskeletal-mitochondria-sr interaction. methods ... | 2017 | 29267873 |
| effects of autologous fat and ascs on swine hypertrophic burn scars: a multimodal quantitative analysis. | hypertrophic scar formation is unpredictable and poorly understood, afflicting both the pediatric and adult populations. treatment methods with conservative and invasive approaches have low rates of compliance and high rates of morbidity. the purpose of this study was to test a reproducible scar model and investigate a new technique of scar modification through the use of adipose- derived progenitor stromal cells (ascs). | 2017 | 29263956 |
| spontaneously arrested transmission of cysticercosis in a highly endemic village with a very low migration rate. | taenia soliumcysticercosis is difficult to eliminate without interventions or societal development. atahualpa is a rural ecuadorian village with documented low migration rate, where domestic pig raising is common and human cysticercosis is endemic. to assess neurocysticercosis (ncc) prevalence, 1,273 villagers aged ≥ 20 years underwent neuroimaging studies, which showed calcified lesions in 121 (9.5%) individuals, but no active disease. likewise, positive reactions, apparently nonspecific, were ... | 2017 | 29260655 |
| the silent spread of porcine bocavirus in croatian pigs: should we be concerned? | a survey was conducted to evaluate the presence and prevalence of porcine bocavirus (pbov) in croatian domestic pigs by means of pcr targeting the ns1 gene fragment of pbov. this study included testing of faecal samples collected from 10 small commercial farms and 11 small backyard holdings in croatia. the presence of pbov was confirmed by pcr in 24 out of 57 composite faecal samples from small commercial farms and in 12 out of 43 composite faecal samples from small backyard holdings. the pcr pr ... | 2017 | 29256289 |
| inferring the evolution of the major histocompatibility complex of wild pigs and peccaries using hybridisation dna capture-based sequencing. | the major histocompatibility complex (mhc) is a key genomic model region for understanding the evolution of gene families and the co-evolution between host and pathogen. to date, mhc studies have mostly focused on species from major vertebrate lineages. the evolution of mhc classical (ia) and non-classical (ib) genes in pigs has attracted interest because of their antigen presentation roles as part of the adaptive immune system. the pig family suidae comprises over 18 extant species (mostly wild ... | 2017 | 29256177 |
| species specific antiviral activity of porcine interferon-α8 (ifnα8). | interferons (ifns) have been known as antiviral genes and they are classified by type 1, type 2, and type 3 ifn. the type 1 ifn consists of ifnα, ifnβ, ifnτ, and ifnω whereas the type 2 ifn consists of only ifnγ, which is a key cytokine driving t helper cell type 1 immunity. ifnλ belongs to the type 3 ifn, which is also known as il-28 and il-29 possessing antiviral activities. type 1 ifn is produced by viral infection whereas type 2 ifn is induced by mitogenic or antigenic t-cell stimuli. the if ... | 2017 | 29302255 |
| a prototype single-port device for pressurized intraperitoneal aerosol chemotherapy. technical feasibility and local drug distribution. | to evaluate the technical feasibility and homogeneity of drug distribution of pressurized intraperitoneal aerosol chemotherapy (pipac) based on a novel process of intraperitoneal drug application (multidirectional aerosolization). | 2017 | 29319734 |
| efficacy and safety of sic-8000 (eleview®) for submucosal injection for endoscopic mucosal resection and endoscopic submucosal dissection in an in vivo porcine model. | submucosal injection is generally required for both endoscopic-mucosal resection (emr) and submucosal dissection (esd). sic-8000 (eleview™) is a new fda 510(k) cleared and ce marked liquid composition for submucosal injection, containing a biocompatible polymer as a cushioning agent. | 2017 | 29396133 |
| feasibility of electromagnetically guided transjugular intrahepatic portosystemic shunt procedure. | to develop an electromagnetic navigation technology for transjugular intrahepatic portosystemic shunt (tips) creation and translate it from phantom to an in-vivo large animal setting. | 2017 | 27686414 |
| the 'heritability' of domestication and its functional partitioning in the pig. | we propose to estimate the proportion of variance explained by regression on genome-wide markers (or genomic heritability) when wild/domestic status is considered the phenotype of interest. this approach differs from the standard fst in that it can accommodate genetic similarity between individuals in a general form. we apply this strategy to complete genome data from 47 wild and domestic pigs from asia and europe. when we partitioned the total genomic variance into components associated to subs ... | 2017 | 27649617 |
| a simulation model to determine sensitivity and timeliness of surveillance strategies. | animal surveillance systems need regular evaluation. we developed an easily applicable simulation model of the german wild boar population to investigate two evaluation attributes: the sensitivity and timeliness (i.e. the ability to detect a disease outbreak rapidly) of a surveillance system. classical swine fever (csf) was used as an example for the model. csf is an infectious disease that may lead to massive economic losses. it can affect wild boar as well as domestic pigs, and csf outbreaks i ... | 2017 | 27619421 |
| influence of on-farm pig salmonella status on salmonella shedding at slaughter. | the risk of salmonella shedding among pigs at slaughter with regard to their previous on-farm salmonella status was assessed in a group of pigs from a farm from ne of spain. a total of 202 pigs that had been serologically monitored monthly during the fattening period and from which mesenteric lymph nodes (mln) and faecal (sfec) samples were collected at slaughter for salmonella isolation were included. a repeated-measures anova was used to assess the relationship between mean od% values during t ... | 2017 | 27549508 |
| periconceptional undernutrition affects in utero methyltransferase expression and steroid hormone concentrations in uterine flushings and blood plasma during the peri-implantation period in domestic pigs. | female undernutrition during early pregnancy may affect the physiological pattern of genomic dna methylation. we hypothesised that in utero dna methylation may be impaired in females fed a restrictive diet in early pregnancy. in this study we evaluated whether poor maternal nutritional status, induced by applying a restricted diet during the peri-conceptional period, may influence: (1) the potential for in utero dna methylation, expressed as changes in the mrna expression and protein abundance o ... | 2017 | 27534526 |
| cholinergic profiles in the goettingen miniature pig (sus scrofa domesticus) brain. | central cholinergic structures within the brain of the even-toed hoofed goettingen miniature domestic pig (sus scrofa domesticus) were evaluated by immunohistochemical visualization of choline acetyltransferase (chat) and the low-affinity neurotrophin receptor, p75ntr. chat-immunoreactive (-ir) perikarya were seen in the olfactory tubercle, striatum, medial septal nucleus, vertical and horizontal limbs of the diagonal band of broca, and the nucleus basalis of meynert, medial habenular nucleus, z ... | 2017 | 27490949 |
| a cartographic tool for managing african swine fever in eurasia: mapping wild boar distribution based on the quality of available habitats. | the current african swine fever (asf) epidemic in eurasia represents a risk for the swine industry with devastating socio-economic and political consequences. wild boar appears to be a key factor in maintaining the disease in endemic areas (mainly the russian federation) and spreading the disease across borders, including within the european union. to help predict and interpret the dynamics of asf infection, we developed a standardized distribution map based on global land cover vegetation (glob ... | 2017 | 27596983 |
| histopathological comparative analysis of periimplant bone inflammatory response after dental implant insertion using flap and flapless surgical technique. an experimental study in pigs. | to evaluate by histopathological analysis the peri-implant bone inflammation degree, in certain time intervals (7, 14, 21 and 28 days), following mini-incision flapless and flap implant placement. | 2017 | 27393033 |
| still room for improvement: preclinical and bench testing of a thin-strut cobalt-chromium bare-metal stent with passive coating. | there is a renewed interest in bare-metal stent (bms) design as degradable polymer coatings are increasingly used as drug-delivery vehicles in drug-eluting stents (dess), leaving it to the bms platform to determine the long-term outcome of des treatment. in this study comprising preclinical and bench tests, we compare two modern thin-strut bmss of different design. angiography, morphometry, and histopathology data were acquired in a porcine coronary artery model in a 28-day single-stent study (1 ... | 2017 | 27149341 |
| biodegradable polymeric stents for vascular application in a porcine carotid artery model: english version. | over the past years the development of biodegradable polymeric stents has made great progress; nevertheless, essential problems must still be solved. modifications in design and chemical composition should optimize the quality of biodegradable stents and remove the weaknesses. new biodegradable poly-l-lactide/poly-4-hydroxybutyrate (plla/p4hb) stents and permanent 316l stents were implantedendovascularly into both common carotid arteries of 10 domestic pigs. at 4 weeks following implantation, co ... | 2017 | 27034581 |
| a simple porcine model of inducible sustained atrial fibrillation. | the surgical management of atrial fibrillation (af) is an evolving field with a history of testing various lesion sets and ablation technologies. previous animal models of af require a chronic intervention to make af reliably inducible. our objective was to create an acute, reliable, and reproducible porcine model of sustained af. to accomplish this, 21 adult domestic pigs underwent median sternotomy. methods to induce af were then performed sequentially: manual stimulation, rapid pacing (200 be ... | 2017 | 26889882 |
| dynamics of myosin heavy chain isoform transition in the longissimus muscle of domestic and wild pigs during growth: a comparative study. | dynamics of myofiber differentiation/maturation in porcine skeletal muscle is associated with domestication, breeding and rearing conditions. this study was aimed to comparatively elucidate the age-dependent myosin heavy chain (myhc) isoform expression and transition pattern in domestic and wild pig (wp) skeletal muscle from birth until adulthood. domestic pigs (dps) of large white breed raised in conventional production system were compared with wps reared in a large hunting enclosure. muscle s ... | 2017 | 27345286 |
| effects of carbon dioxide insufflation during direct cholangioscopy on biliary pressures and vital parameters: a pilot study in porcine models. | direct per-oral cholangioscopy allows endoscopic visualization of the biliary tract. insufflation with carbon dioxide (co2) is an alternative to saline solution irrigation during direct cholangioscopy. there are no data on maximal co2insufflation in direct cholangioscopy without causing biliary barotrauma or fatal gas embolism. we aimed to evaluate the safety of increasing co2insufflation in direct cholangioscopy without causing biliary barotrauma or fatal gas embolism. | 2017 | 27327853 |
| spatio-temporal analysis of african swine fever in sardinia (2012-2014): trends in domestic pigs and wild boar. | african swine fever (asf) is a notifiable viral disease affecting domestic pigs and wild boars that has been endemic in sardinia since 1978. several risk factors complicate the control of asf in sardinia: generally poor level of biosecurity, traditional breeding practices, illegal behaviour in movements and feeding of pigs, and sporadic occurrence of long-term carriers. a previous study describes the disease in sardinia during 1978-2013. the aim of this study was to gain more in-depth knowledge ... | 2017 | 26392004 |
| statistical exploration of local transmission routes for african swine fever in pigs in the russian federation, 2007-2014. | african swine fever (asf) is a devastating viral disease of swine that is present in both pigs and wild boar in the western part of the russian federation and the eastern part of the european union. it represents a significant threat for the european pig production industry as neither treatment nor vaccine is available. this study analysed the spatial and spatio-temporal distributions of asf cases that were reported in domestic pigs and wild boar for assessing the likelihood of wild boar-to-dome ... | 2017 | 26192820 |
| neurophysiological monitoring during acute and progressive experimentally induced compression injury of the spinal cord in pigs. | to evaluate the degree of acute or progressive lateral compression needed to cause neurologic injury to the spinal cord assessed by electrophysiological monitoring. | 2017 | 25862652 |
| performance of a novel bipolar/monopolar radiofrequency ablation device on the beating heart in an acute porcine model. | although the advent of ablation technology has simplified and shortened surgery for atrial fibrillation, only bipolar clamps have reliably been able to create transmural lesions on the beating heart. currently, there are no devices capable of reproducibly creating the long linear lesions in the right and left atria needed to perform a cox-maze procedure. this study evaluated the performance of a novel suction-assisted radiofrequency device that uses both bipolar and monopolar energy to create le ... | 2017 | 24145972 |
| the effect of high-fat diet on the composition of the gut microbiota in cloned and non-cloned pigs of lean and obese phenotype. | the aim of this study was to investigate the effect of high-far-high-energy diet on cloned and non-cloned domestic pigs of both lean and obese phenotype and to evaluate if the lean cloned pigs had a lower inter-individual variation as compared with non-cloned pigs. the microbiota of colon and terminal ileum was investigated in cloned and non-cloned pigs that received a high-far-high-energy diet with either restricted or ad libitum access to feed, resulting in lean and obese phenotypes, respectiv ... | 2017 | 23974297 |
| 1ppab5. acoustic radiation force to reposition kidney stones. | our group has introduced transcutaneous ultrasound to move kidney stones in order to expel small stones or relocate an obstructing stone to a nonobstructing location. human stones and metalized beads (2-8 mm) were implanted ureteroscopically in kidneys of eight domestic swine. ultrasonic propulsion was performed using a diagnostic imaging transducer and a verasonics ultrasound platform. stone propulsion was visualized using fluoroscopy, ultrasound, and the ureteroscope. successful stone movement ... | 2017 | 26207161 |
| seroprevalence of anti-hepatitis e virus antibodies in domestic pigs in mexico. | hepatitis e virus (hev) infection is one of the most common causes of acute liver diseases in humans worldwide. in developing countries, hev is commonly associated with waterborne outbreaks. conversely, in industrialized countries, hev infection is often associated with travel to endemic regions or ingestion of contaminated animal products. limited information on both, human and animal hev infection in mexico is available. as a consequence, the distribution of the virus in the country is largely ... | 2017 | 28934965 |
| a novel culture method reveals unique neural stem/progenitors in mature porcine iris tissues that differentiate into neuronal and rod photoreceptor-like cells. | iris neural stem/progenitor cells from mature porcine eyes were investigated using a new protocol for tissue culture, which consists of dispase treatment and matrigel embedding. we used a number of culture conditions and found an intense differentiation of neuronal cells from both the iris pigmented epithelial (ipe) cells and the stroma tissue cells. rod photoreceptor-like cells were also observed but mostly in a later stage of culture. neuronal differentiation does not require any additives suc ... | 2017 | 28866055 |
| genetics of body fat mass and related traits in a pig population selected for leanness. | obesity is characterized as the excessive accumulation of body fat and has a complex genetic foundation in humans including monogenic high-risk mutations and polygenic contributions. domestic pigs represent a valuable model on an obesity-promoting high-caloric diet while constantly evaluated for body characteristics. as such, we investigated the genetics of obesity-related traits, comprising subcutaneous fat thickness, lean mass percentage, and growth rate, in a pig population. we conducted geno ... | 2017 | 28831160 |
| cross-species transmission potential between wild pigs, livestock, poultry, wildlife, and humans: implications for disease risk management in north america. | cross-species disease transmission between wildlife, domestic animals and humans is an increasing threat to public and veterinary health. wild pigs are increasingly a potential veterinary and public health threat. here we investigate 84 pathogens and the host species most at risk for transmission with wild pigs using a network approach. we assess the risk to agricultural and human health by evaluating the status of these pathogens and the co-occurrence of wild pigs, agriculture and humans. we id ... | 2017 | 28798293 |
| genetically modified pigs as organ donors for xenotransplantation. | the growing shortage of available organs is a major problem in transplantology. thus, new and alternative sources of organs need to be found. one promising solution could be xenotransplantation, i.e., the use of animal cells, tissues and organs. the domestic pig is the optimum donor for such transplants. however, xenogeneic transplantation from pigs to humans involves high immune incompatibility and a complex rejection process. the rapid development of genetic engineering techniques enables geno ... | 2017 | 28698981 |
| coping style modifies general and affective autonomic reactions of domestic pigs in different behavioral contexts. | based on individual adaptive strategies (coping), animals may react differently to environmental challenges in terms of behavior and physiology according to their emotional perception. emotional valence as well as arousal may be derived by measuring vagal and sympathetic tone of the autonomic nervous system (ans). we investigated the situation-dependent autonomic response of 16 domestic pigs with either a reactive or a proactive coping style, previously selected according to the backtest which i ... | 2017 | 28611608 |
| the yucatan minipig temporomandibular joint disc structure-function relationships support its suitability for human comparative studies. | frequent involvement of the disc in temporomandibular joint (tmj) disorders warrants attempts to tissue engineer tmj disc replacements. physiologically, a great degree of similarity is seen between humans and farm pigs (fps), but the pig's rapid growth confers a significant challenge for in vivo experiments. minipigs have a slower growth rate and are smaller than fps, but minipig tmj discs have yet to be fully characterized. the objective of this study was to determine the suitability of the min ... | 2017 | 28548559 |
| sero-prevalence of specific leptospira serovars in fattening pigs from 5 provinces in vietnam. | leptospirosis is a zoonotic bacterial disease with a worldwide distribution. in vietnam, leptospirosis is considered endemic. in pigs, leptospirosis can result in reproductive problems (such as abortion and infertility) which lead to economic loss. in addition, transmission to people presents a public health risk. in vietnam, few national studies have been conducted on sero-prevalence of leptospirosis in pigs. the main objective of this study was to evaluate the sero-prevalence and incidence of ... | 2017 | 28482843 |
| noninvasive neuromodulation and thalamic mapping with low-intensity focused ultrasound. | objective ultrasound can be precisely focused through the intact human skull to target deep regions of the brain for stereotactic ablations. acoustic energy at much lower intensities is capable of both exciting and inhibiting neural tissues without causing tissue heating or damage. the objective of this study was to demonstrate the effects of low-intensity focused ultrasound (lifu) for neuromodulation and selective mapping in the thalamus of a large-brain animal. methods ten yorkshire swine ( su ... | 2017 | 28430035 |
| localization of the corticospinal tract within the porcine spinal cord: implications for experimental modeling of traumatic spinal cord injury. | spinal cord injury (sci) researchers have predominately utilized rodents for sci modeling and experimentation. unfortunately, a large number of novel therapies developed in rodent models have failed to demonstrate efficacy in human clinical trials which suggests that improved animal models are an important translational tool. recently, porcine models of sci have been identified as a valuable intermediary model for preclinical evaluation of promising therapies to aid clinical translation. however ... | 2017 | 28323088 |
| a domestic porcine model for studying the effects of radiation on head and neck cancers. | radiation therapy (rt) of the head and neck region is often accompanied by serious side effects. research in this area is needed to improve treatment outcomes and ameliorate therapy tolerance. laboratory rodents are barely matching today's clinical standards in rt research. yet domestic swine (sus scrofa domestica) have previously proved suitable for various advanced tests in clinical research and training. we therefore investigated whether s. scrofa domestica is also appropriate for irradiation ... | 2017 | 28130028 |
| occurrence and first molecular characterization of sarcocystis spp. in wild boars (sus scrofa) and domestic pigs (sus scrofa domesticus) in romania: public health significance of the isolates. | domestic and wild pigs, as intermediate hosts, can harbor tissue cysts of three sarcocystis species namely s. miescheriana, s. suihominis and s. porcifelis. out of them, s. suihominis is zoonotic. romania is a country with high consumption of raw and/or undercooked traditional pork products. this fact may greatly favor the acquiring of the zoonotic sarcocystis infections by humans, as definitive host. based on this consideration and in order to investigate the occurrence and public health signif ... | 2017 | 28041999 |
| azoospermia and testicular hypoplasia in a boar carrier of a novel y-autosome translocation. | few sex-autosome chromosome abnormalities have been documented in domestic animal species. in humans, y-autosome chromosome abnormalities may occur at a rate of 1/2,000 live births, whereas in the domestic pig only 2 y-autosome reciprocal translocations have been previously described. during a routine cytogenetic screening of young boars, we identified a new y-autosome translocation carrier, which after puberty showed semen devoid of sperm and testicular hypoplasia with spermatogenesis arrest. w ... | 2017 | 27974725 |
| utilization of mri for cerebral white matter injury in a hypobaric swine model-validation of technique. | repetitive hypobaric exposure in humans induces subcortical white matter change, observable on magnetic resonance imaging (mri) and associated with cognitive impairment. similar findings occur in traumatic brain injury (tbi). we are developing a swine mri-driven model to understand the pathophysiology and to develop treatment interventions. | 2017 | 29087921 |
| prevalence and risk factors of japanese encephalitis virus (jev) in livestock and companion animal in high-risk areas in malaysia. | japanese encephalitis (je) is vector-borne zoonotic disease which causes encephalitis in humans and horses. clinical signs for japanese encephalitis virus (jev) infection are not clearly evident in the majority of affected animals. in malaysia, information on the prevalence of jev infection has not been established. thus, a cross-sectional study was conducted during two periods, december 2015 to january 2016 and march to august in 2016, to determine the prevalence and risk factors in jev infecti ... | 2017 | 29243139 |
| serological evidence of hepatitis e virus infection in zoo animals and identification of a rodent-borne strain in a syrian brown bear. | hepatitis e virus (hev) is the causative agent of hepatitis e, an emerging infectious disease of humans. hev infections have also been described in various animal species. whereas domestic pigs and wild boars are well-known animal reservoirs for hev, the knowledge on natural hev infection in zoo animals is scarce so far. here, we analysed 244 sera from 66 mammal species derived from three zoos in germany using a commercial double antigen sandwich elisa. hev-specific antibodies were detected in 1 ... | 2017 | 29173594 |
| identification of tick species collected from wild boars and habitats of wild boars and domestic pigs in the republic of korea. | tick is one of the most important arthropods in the transmission of vector-borne diseases. in this study, we investigated the abundance and species of ticks associated with swine and their habitats to assess the risk of spread of tick-borne diseases in host species, such as wild boars. ticks were collected from 24 grazing or traditionally reared domestic pig farms and 8 habitats of wild boars in 8 provinces and 1 city in the republic of korea, by using the dragging and flagging methods. ticks we ... | 2017 | 28506041 |