Publications

infections of virulent and avirulent viruses differentially influenced the expression of dicer-1, ago-1, and micrornas in bombus terrestris.	the microrna (mirna) pathway is well established to be involved in host-pathogen interactions. as key insect pollinators, bees are suffering from widely spreading viruses, especially honeybees and bumblebees. in order to better understand bee-virus interaction, we comparatively analyzed the involvement of the bumblebee mirna pathway upon infection by two different viruses. in our setup, an avirulent infection is induced by slow bee paralysis virus (sbpv) and a virulent infection is induced by is ...	2017	28374846
in search of evidence for the experience of pain in honeybees: a self-administration study.	despite their common use as model organisms in scientific experiments, pain and suffering in insects remains controversial and poorly understood. here we explore potential pain experience in honeybees (apis mellifera) by testing the self-administration of an analgesic drug. foragers were subjected to two different types of injuries: (i) a clip that applied continuous pressure to one leg and (ii) amputation of one tarsus. the bees were given a choice between two feeders, one offering pure sucrose ...	2017	28374827
three years of banning neonicotinoid insecticides based on sub-lethal effects: can we expect to see effects on bees?	the 2013 eu ban of three neonicotinoids used in seed coating of pollinator attractive crops was put in place because of concern about declining wild pollinator populations and numbers of honeybee colonies. it was also concluded that there is an urgent need of good field data to fill knowledge gaps. in the meantime such data have been generated. based on recent literature we question the existence of recent pollinator declines and their possible link with the use of neonicotinoids. because of tem ...	2017	28374565
free-flying honeybees extrapolate relational size rules to sort successively visited artificial flowers in a realistic foraging situation.	learning and applying relational concepts to solve novel tasks is considered an indicator of cognitive-like ability. it requires the abstraction of relational concepts to different objects independent to the physical nature of the individual objects. recent research has revealed the honeybee's ability to rapidly learn and manipulate relations between visual stimuli such as 'same/different', 'above/below', or 'larger/smaller' despite having a miniature-sized brain. while honeybees can solve probl ...	2017	28374206
a novel interval type-2 fractional order fuzzy pid controller: design, performance evaluation, and its optimal time domain tuning.	in this paper, a novel concept of an interval type-2 fractional order fuzzy pid (it2fo-fpid) controller, which requires fractional order integrator and fractional order differentiator, is proposed. the incorporation of takagi-sugeno-kang (tsk) type interval type-2 fuzzy logic controller (it2flc) with fractional controller of pid-type is investigated for time response measure due to both unit step response and unit load disturbance. the resulting it2fo-fpid controller is examined on different del ...	2017	28372800
longevity extension of worker honey bees (apis mellifera) by royal jelly: optimal dose and active ingredient.	in the western honey bee, apis mellifera, queens and workers have different longevity although they share the same genome. queens consume royal jelly (rj) as the main food throughout their life, including as adults, but workers only eat worker jelly when they are larvae less than 3 days old. in order to explore the effect of rj and the components affecting longevity of worker honey bees, we first determined the optimal dose for prolonging longevity of workers as 4% rj in 50% sucrose solution, an ...	2017	28367370
the effect of nest box distribution on sustainable propagation of osmia lignaria (hymenoptera: megachilidae) in commercial tart cherry orchards.	the blue orchard bee, osmia lignaria (say), is a solitary bee that is an excellent pollinator of tree fruit orchards. due to the annual rising costs of honey bee hive rentals, many orchardists are eager to develop management tools and practices to support o. lignaria as an alternative pollinator. establishing o. lignaria pollination as a sustainable industry requires careful consideration of both bee and orchard management. here, we test the effect of artificial nest box distribution on in-orcha ...	2017	28365763
caffeic acid phenethyl ester protects against glucocorticoid-induced osteoporosis in vivo: impact on oxidative stress and rankl/opg signals.	glucocorticoid-induced osteoporosis (gio) is one of the most common causes of secondary osteoporosis. given that glucocorticoids are considered as a main component of the treatment protocols for a variety of inflammation and immune-mediated diseases besides its use as adjuvant to several chemotherapeutic agents, it is crucial to find ways to overcome this critical adverse effect. caffeic acid phenethyl ester (cape), which is a natural compound derived from honeybee propolis displayed promising a ...	2017	28363435
effect of major royal jelly proteins on spatial memory in aged rats: metabolomics analysis in urine.	royal jelly (rj) produced by worker honeybees is the sole food for the queen bee throughout her life as well as the larvae of worker bees for the first 3 days after hatching. supplementation of rj in the diet has been shown to increase spatial memory in rodents. however, the key constituents in rj responsible for improvement of cognitive function are unknown. our objective was to determine if the major royal jelly proteins (mrjps) extracted from rj can improve the spatial memory of aged rats. th ...	2017	28362493
micrornas associated with caste determination and differentiation in a primitively eusocial insect.	in eusocial hymenoptera (ants, bees and wasps), queen and worker adult castes typically arise via environmental influences. a fundamental challenge is to understand how a single genome can thereby produce alternative phenotypes. a powerful approach is to compare the molecular basis of caste determination and differentiation along the evolutionary trajectory between primitively and advanced eusocial species, which have, respectively, relatively undifferentiated and strongly differentiated adult c ...	2017	28361900
condition-dependent virulence of slow bee paralysis virus in bombus terrestris: are the impacts of honeybee viruses in wild pollinators underestimated?	slow bee paralysis virus (sbpv)-previously considered an obligate honeybee disease-is now known to be prevalent in bumblebee species. sbpv is highly virulent in honeybees in association with varroa mites, but has been considered relatively benign otherwise. however, condition-dependent pathogens can appear asymptomatic under good, resource abundant conditions, and negative impacts on host fitness may only become apparent when under stressful or resource-limited conditions. we tested whether sbpv ...	2017	28361244
common antibiotic hurts bee survival.		2017	28360316
a case series of bee sting keratopathy with different outcomes in malaysia.	we report three patients with corneal bee sting at our tertiary care center in a three-year period starting from 2014 to 2016. all patients sustained a bee sting injury to the cornea. all patients received early preoperative topical antibiotics, topical cycloplegic and intensive topical steroids. however, the timing of the initial presentation, the duration, and the location of the retained stinger differed in each case leading to different postsurgical outcomes.	2017	28357167
melittin inhibits the invasion of mcf-7 cells by downregulating cd147 and mmp-9 expression.	tumor invasion and metastasis are the critical steps in determining the aggressive phenotype of human cancers. melittin, a major component of bee venom, has been reported to induce apoptosis in several cancer cells. however, the mechanisms of melittin involvement in cancer invasion and metastasis remain unclear. our previous study indicated that melittin inhibits cyclophilin a (cypa), a ubiquitously distributed peptidylprolyl cis-trans isomerase, in macrophage cells. in the present study, the tr ...	2017	28356935
the dynamic association between ovariole loss and sterility in adult honeybee workers.	in the social insects, ovary state (the presence or absence of mature oocytes) and ovary size (the number of ovarioles) are often used as proxies for the reproductive capacity of an individual worker. ovary size is assumed to be fixed post-eclosion whereas ovary state is demonstrably plastic post-eclosion. here, we show that in fact ovary size declines as honeybee workers age. this finding is robust across two honeybee species: apis mellifera and a. cerana the ovariole loss is likely to be due t ...	2017	28356452
biophysical characterization of the varroa destructor nav1 sodium channel and its affinity for τ-fluvalinate insecticide.	the decline of the western honeybee (apis mellifera) has been reported to be due to parasitism by varroa destructor mites and to colony collapse disorder in which these mites may be involved. in-hive chemicals such as τ-fluvalinate are being used to control vdestructor populations. this approach may lead to the chronic exposure of bees to this liposoluble chemical, which tends to accumulate in hives. we cloned a variant of the v. destructor sodium channel (vdnav1) and studied its biophysical cha ...	2017	28356346
drag reduction in a natural high-frequency swinging micro-articulation: mouthparts of the honey bee.	worker-bee mouthparts consist of the glossa, the galeae and the vestigial labial palp, and it is these structures that enable bees to feed themselves. the articulation joints, 60∼70 µm in diameter, are present on the tip of the labial palp and are covered with olfactory sensilla, allowing movements between the segments. using a specially designed high-speed camera system, we discovered that the articulation joint could swing in the nectar at a frequency of ∼50 hz, considerably higher than the us ...	2017	28355472
food consumption and food exchange of caged honey bees using a radioactive labelled sugar solution.	we measured the distribution of sugar solution within groups of caged honey bees (apis mellifera) under standard in vitro laboratory conditions using 14c polyethylene glycol as a radioactive marker to analyze ingestion by individual bees after group feeding. we studied the impact of different experimental setups by varying the number of bees, age of bees, origin of bees, duration of experiment, the amount of available diet, and the influence of the neurotoxic pesticide imidacloprid in the diet o ...	2017	28355267
melittin inhibits osteoclast formation through the downregulation of the rankl-rank signaling pathway and the inhibition of interleukin-1β in murine macrophages.	melittin is a major toxic component of bee venom (apis mellifera). it is not known whether melittin is involved in bone metabolism and osteoclastogenesis. the aim of this study was to determine the role of melittin in the regulation of osteoclastogenesis. in vitro osteoclastogenesis assays were performed using mouse raw 264.7 cells and bone marrow-derived macrophages (bmms) treated with receptor activator of nuclear factor-κb ligand (rankl) and macrophage colony-stimulating factor (m-csf). morph ...	2017	28350111
improvement of identification methods for honeybee specific lactic acid bacteria; future approaches.	honeybees face many parasites and pathogens and consequently rely on a diverse set of individual and group-level defenses to prevent disease. the crop microbiota of apis mellifera, composed of 13 lactic acid bacterial (lab) species within the genera lactobacillus and bifidobacterium, form a beneficial symbiotic relationship with each other and the honeybee to protect their niche and their host. possibly playing a vital role in honeybee health, it is important that these honeybee specific lactic ...	2017	28346815
six years of instand e. v. sige proficiency testing: an evaluation of in vitro allergy diagnostics.	even though allergies are an important health issue, wide manufacturer-dependent differences in the detected amounts of allergen-specific ige (sige) have repeatedly been found. these discrepancies hinder diagnostics and research into clinically significant cutoff points for life-threatening symptoms.	2017	28344920
direct interaction between caffeic acid phenethyl ester and human neutrophil elastase inhibits the growth and migration of panc-1 cells.	pancreatic ductal adenocarcinoma (pdac) is one of the most lethal malignant tumors of the digestive system, but the mechanisms of its development and progression are unclear. inflammation is thought to be fundamental to pancreatic cancer development and caffeic acid phenethyl ester (cape) is an active component of honey bee resin or propolis with anti-inflammatory and anticancer activities. we investigated the inhibitory effects of cape on cell growth and migration induced by human neutrophil el ...	2017	28339071
antioxidant and anti-tyrosinase activities of phenolic extracts from rape bee pollen and inhibitory melanogenesis by camp/mitf/tyr pathway in b16 mouse melanoma cells.	rape bee pollen possesses many nutritional and therapeutic properties because of its abundant nutrimental and bioactive components. in this study, free (fpe) and bound (bpe) phenolic extracts of rape bee pollen were obtained, phenolic and flavonoid contents were determined, and composition of phenolic acids was analyzed. in vitro antioxidant and anti-tyrosinase (tyr) activities of fpe and bpe were compared, and inhibitory melanogenesis of fpe was further evaluated. results showed fpe and bpe con ...	2017	28337140
erratum to: unity in defence: honeybee workers exhibit conserved molecular responses to diverse pathogens.		2017	28335723
pollinator identity and spatial isolation influence multiple paternity in an annual plant.	the occurrence and extent of multiple paternity is an important component of variation in plant mating dynamics. however, links between pollinator activity and multiple paternity are generally lacking, especially for plant species that attract functionally diverse floral visitors. in this study, we separated the influence of two functionally distinct floral visitors (hawkmoths and solitary bees) and characterized their impacts on multiple paternity in a self-incompatible, annual forb, oenothera ...	2017	28334485
a bio-economic case study of canadian honey bee (hymenoptera: apidae) colonies: marker-assisted selection (mas) in queen breeding affects beekeeper profits.	over the past decade in north america and europe, winter losses of honey bee (hymenoptera: apidae) colonies have increased dramatically. scientific consensus attributes these losses to multifactorial causes including altered parasite and pathogen profiles, lack of proper nutrition due to agricultural monocultures, exposure to pesticides, management, and weather. one method to reduce colony loss and increase productivity is through selective breeding of queens to produce disease-, pathogen-, and ...	2017	28334400
the synergistic effects of almond protection fungicides on honey bee (hymenoptera: apidae) forager survival.	the honey bee (apis mellifera l.) contributes ∼$17 billion annually to the united states economy, primarily by pollinating major agricultural crops including almond, which is completely dependent on honey bee pollination for nut set. almond growers face constant challenges to crop productivity owing to pests and pathogens, which are often controlled with a multitude of agrochemicals. for example, fungicides are often applied in combination with other products to control fungal pathogens during a ...	2017	28334292
population growth of varroa destructor (acari: varroidae) in colonies of russian and unselected honey bee (hymenoptera: apidae) stocks as related to numbers of foragers with mites.	varroa (varroa destructor anderson and trueman) is an external parasite of honey bees (apis mellifera l.) and a leading cause of colony losses worldwide. varroa populations can be controlled with miticides, but mite-resistant stocks such as the russian honey bee (rhb) also are available. russian honey bee and other mite-resistant stocks limit varroa population growth by affecting factors that contribute to mite reproduction. however, mite population growth is not entirely due to reproduction. nu ...	2017	28334279
ecology, life history, and management of tropilaelaps mites.	parasitic mites are the major threat to the western honey bee, apis mellifera l. for much of the world, varroa destructor anderson & trueman single-handedly inflicts unsurmountable problems to a. mellifera beekeeping. however, a. mellifera in asia is also faced with another genus of destructive parasitic mite, tropilaelaps. the life history of these two parasitic mites is very similar, and both have the same food requirements (i.e., hemolymph of developing brood). hence, parasitism by tropilaela ...	2017	28334185
managed bumble bees (bombus impatiens) (hymenoptera: apidae) caged with blueberry bushes at high density did not increase fruit set or fruit weight compared to open pollination.	highbush blueberry (vaccinium corymbosum l.) is an important crop grown throughout florida. currently, most blueberry growers use honey bees (apis mellifera l.) to provide pollination services for highbush blueberries even though bumble bees (bombus spp.) have been shown to be more efficient at pollinating blueberries on a per bee basis. in general, contribution of bumble bees to the pollination of commercial highbush blueberries in florida is unknown. herein, we determined if managed bumble bee ...	2017	28334127
floral strips attract beneficial insects but do not enhance yield in cucumber fields.	natural enemies and pollinators require nutritional and habitat resources that are often not found in conventional agricultural fields. the addition of flowering plants within agroecosystems may provide the resources necessary to support beneficial insects at the local scale. we hypothesized that insect pollinator and natural enemy abundance would increase in cucumber (cucumis sativus) plots containing flower strips and that the effect would be greatest in the crop rows closest to the flower str ...	2017	28334107
honey bee hairs and pollenkitt are essential for pollen capture and removal.	while insect grooming has been observed and documented for over one hundred years, we present the first quantitative analysis of this highly dynamic process. pollinating insects, like honey bees, purposely cover themselves with millions of pollen particles that, if left ungroomed, would make sensing and controlled flight difficult. how do they get clean? we show that the hairs on insect eyes are tuned to the pollen they collect; namely, the hairs are spaced so that they suspend pollen above the ...	2017	28332480
pollinator population size and pollination ecosystem service responses to enhancing floral and nesting resources.	modeling pollination ecosystem services requires a spatially explicit, process-based approach because they depend on both the behavioral responses of pollinators to the amount and spatial arrangement of habitat and on the within- and between-season dynamics of pollinator populations in response to land use. we describe a novel pollinator model predicting flower visitation rates by wild central-place foragers (e.g., nesting bees) in spatially explicit landscapes. the model goes beyond existing ap ...	2017	28331597
a clinical trial protocol to treat massive africanized honeybee (apis mellifera) attack with a new apilic antivenom.	envenomation caused by multiple stings from africanized honeybees apis mellifera constitutes a public health problem in the americas. in 2015, the brazilian ministry of health reported 13,597 accidents (incidence of seven cases per 100,000 inhabitants) with 39 deaths (lethality of 0.25%). the toxins present in the venom, which include melittin and phospholipase a2, cause lesions in diverse organs and systems that may be fatal. as there has been no specific treatment to date, management has been ...	2017	28331487
taxonomy of the african large carpenter bees of the genus xylocopa latreille, 1802, subgenus xenoxylocopa hurd & moure, 1963 (hymenoptera, apidae).	the taxonomy of the genus xylocopa latreille, 1802, subgenus xenoxylocopa hurd & moure, 1963, is reviewed. there is a single valid species in this subgenus, xylocopa (xenoxylocopa) inconstans smith, 1874, which is widely distributed throughout sub-saharan africa, from senegal to ethiopia and south to northern republic of south africa. synonyms of xylocopa inconstans include xylocopa abyssinica radoszkowski, 1899, proposed for a male specimen from ethiopia, as well as three names proposed for fem ...	2017	28331398
the genus amegilla (hymenoptera, apidae, anthophorini) in australia: a revision of the subgenera notomegilla and zonamegilla.	the australian bees in the subgenera notomegilla and zonamegilla of the genus amegilla are revised. commonly in australia the species in these subgenera are called blue-banded bees, although not all species have blue bands. a phylogeny based on mitochondrial cytochrome oxidase 1 sequence data was used to delineate the species and a set of morphological criteria was developed for species identification. strong support was obtained for separating the australian species into the three subgenera pre ...	2017	28331394
linking magnetite in the abdomen of honey bees to a magnetoreceptive function.	previous studies of magnetoreception in honey bees, apis mellifera, focused on the identification of magnetic material, its formation, the location of the receptor and potential underlying sensory mechanisms, but never directly linked magnetic material to a magnetoreceptive function. in our study, we demonstrate that ferromagnetic material consistent with magnetite plays an integral role in the bees' magnetoreceptor. subjecting lyophilized and pelletized bee tagmata to analyses by a superconduct ...	2017	28330921
behavioral, transcriptomic and epigenetic responses to social challenge in honey bees.	understanding how social experiences are represented in the brain and shape future responses is a major challenge in the study of behavior. we addressed this problem by studying behavioral, transcriptomic and epigenetic responses to intrusion in honey bees. previous research showed that initial exposure to an intruder provokes an immediate attack; we now show that this also leads to longer-term changes in behavior in the response to a second intruder, with increases in the probability of respond ...	2017	28328153
neuroscience: intelligence in the honeybee mushroom body.	intelligence, in most people's conception, involves combining pieces of evidence to reach non-obvious conclusions. a recent theoretical study shows that intelligence-like brain functions can emerge from simple neural circuits, in this case the honeybee mushroom body.	2017	28324737
c-type allatostatins mimic stress-related effects of alarm pheromone on honey bee learning and memory recall.	as honey bee populations worldwide are declining there is an urgent need for a deeper understanding of stress reactivity in these important insects. our data indicate that stress responses in bees (apis mellifera l.) may be mediated by neuropeptides identified, on the basis of sequence similarities, as allatostatins (asta, astc and astcc). effects of allatostatin injection are compared with stress-related changes in learning performance induced by the honeybee alarm pheromone, isopentylacetate ( ...	2017	28323874
developmental and loco-like effects of a swainsonine-induced inhibition of α-mannosidase in the honey bee, apis mellifera.	deficiencies in lysosomal a-mannosidase (lam) activity in animals, caused either by mutations or by consuming toxic alkaloids, lead to severe phenotypic and behavioural consequences. yet, epialleles adversely affecting lam expression exist in the honey bee population suggesting that they might be beneficial in certain contexts and cannot be eliminated by natural selection.	2017	28321369
a novel melittin nano-liposome exerted excellent anti-hepatocellular carcinoma efficacy with better biological safety.	melittin is the main effective component of bee venom and has extensive biological functions; however, serious side effects have restricted its clinical application. preclinical and clinical studies showed that the main adverse events were allergic reaction and pain at the administration site. to decrease the toxicity, we prepared melittin nano-liposomes by encapsulating melittin with poloxamer 188 and explored the inhibitory activities on liver cancer together with biological safety. here, we s ...	2017	28320480
is floral morphology a good predictor of floral visitors to antirrhineae (snapdragons and relatives)?	the association between plants and flower visitors has been historically proposed as a main factor driving the evolutionary change of both flower and pollinator phenotypes. the considerable diversity in floral morphology within the tribe antirrhineae has been traditionally related to pollinator types. we used empirical data on the flower visitors from 59 antirrhineae taxa from the literature and our own field surveys, which provide an opportunity to test whether flower phenotypes are reliable pr ...	2017	28316136
octopamine and tyramine modulate the thermoregulatory fanning response in honey bees (apis mellifera l.).	biogenic amines regulate the proximate mechanisms underlying most behavior, including those that contribute to the overall success of complex societies. for honey bees, one critical set of behaviors contributing to the welfare of a colony is involved with nest thermoregulation. worker honeybees cool the colony by performing a fanning behavior, the expression of which is largely influenced by response thresholds modulated by the social environment. here, we examined how changes in biogenic amines ...	2017	28314750
insights into the role of age and social interactions on the sexual attractiveness of queens in an eusocial bee, melipona flavolineata (apidae, meliponini).	the attraction of sexual partners is a vital necessity among insects, and it involves conflict of interests and complex communication systems among male and female. in this study, we investigated the developing of sexual attractiveness in virgin queens (i.e., gynes) of melipona flavolineata, an eusocial stingless bee. we followed the development of sexual attractiveness in 64 gynes, belonging to seven age classes (0, 3, 6, 9, 15, 18 days post-emergence), and we also evaluated the effect of diffe ...	2017	28299419
multifractality in individual honeybee behavior hints at colony-specific social cascades: reanalysis of radio-frequency identification data from five different colonies.	honeybees (apis mellifera) exhibit complex coordination and interaction across multiple behaviors such as swarming. this coordination among honeybees in the same colony is remarkably similar to the concept of informational cascades. the multifractal geometry of cascades suggests that multifractal measures of individual honeybee activity might carry signatures of these colony-wide coordinations. the present work reanalyzes time stamps of entrances to and exits from the hive captured by radio-freq ...	2017	28297945
stingless bee honey, the natural wound healer: a review.	the stingless bee is a natural type of bee that exists in almost every continent. the honey produced by this bee has been widely used across time and space. the distinctive feature of this honey is that it is stored naturally in the pot (cerumen), thus contributing to its beneficial properties, especially in the wound healing process.	2017	28291965
antibiotic exposure perturbs the gut microbiota and elevates mortality in honeybees.	gut microbiomes play crucial roles in animal health, and shifts in the gut microbial community structure can have detrimental impacts on hosts. studies with vertebrate models and human subjects suggest that antibiotic treatments greatly perturb the native gut community, thereby facilitating proliferation of pathogens. in fact, persistent infections following antibiotic treatment are a major medical issue. in apiculture, antibiotics are frequently used to prevent bacterial infections of larval be ...	2017	28291793
firewalls in bee nests-survival value of propolis walls of wild cape honeybee (apis mellifera capensis).	the cape bee is endemic to the winter rainfall region of south africa where fires are an integral part of the ecology of the fynbos (heathland) vegetation. of the 37 wild nests in pristine peninsula sandstone fynbos in the cape point section of table mountain national park that have been analyzed so far, only 22 could be accessed sufficiently to determine the existence of a propolis wall of which 68% had propolis walls which entirely enclosed their openings. the analysis of the 37 wild nests rev ...	2017	28289772
stress-mediated allee effects can cause the sudden collapse of honey bee colonies.	the recent rapid decline in global honey bee populations could have significant implications for ecological systems, economics and food security. no single cause of honey bee collapse has yet to be identified, although pesticides, mites and other pathogens have all been shown to have a sublethal effect. we present a model of a functioning bee hive and introduce external stress to investigate the impact on the regulatory processes of recruitment to the forager class, social inhibition and the lay ...	2017	28288794
taxonomic and functional trait diversity of wild bees in different urban settings.	urbanization is one of the major anthropogenic processes contributing to local habitat loss and extirpation of numerous species, including wild bees, the most widespread pollinators. little is known about the mechanisms through which urbanization impacts wild bee communities, or the types of urban green spaces that best promote their conservation in cities. the main objective of this study was to describe and compare wild bee community diversity, structure, and dynamics in two canadian cities, m ...	2017	28286711
effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: a randomized, double-blind clinical trial.	propolis, a natural resinous substance made by bees from material extracted from plants, flowers and bee's wax, has shown great therapeutic effects and been widely used in food and drug industries. recently, some researchers have studied the effect of this substance in the treatment of diabetes.	2017	28285617
pharmacist use of the electronic medical record to identify adults at risk for anaphylaxis without epinephrine for self-administration.	to describe an innovative pharmacist-led approach, with the use of electronic medical record (emr) data, to identify patients at risk of anaphylaxis in need of epinephrine auto-injector (eai) for self-administration.	2017	28285065
agricultural pesticides and veterinary substances in uruguayan beeswax.	over the last decade, uruguay has expanded and intensified its rainfed crop production. this process has affected beekeeping in several ways: for example, by reducing the space available. this has increased the density of apiaries, the risk of varroosis and acaricide use. additionally, the dominance of no-tillage crops has increased the frequencies of application and of loads of pesticides in regions where such crops share the land with beekeeping and honey production. therefore, the exposure of ...	2017	28284118
bee community of commercial potato fields in michigan and bombus impatiens visitation to neonicotinoid-treated potato plants.	we conducted a bee survey in neonicotinoid-treated commercial potato fields using bowl and vane traps in the 2016 growing season. traps were placed outside the fields, at the field edges, and 10 and 30 m into the fields. we collected 756 bees representing 58 species, with lasioglossum spp. comprising 73% of all captured bees. we found seven bombus spp., of which b. impatiens was the only known visitor of potato flowers in our region. the majority of the bees (68%) were collected at the field edg ...	2017	28282931
agrochemical synergism imposes higher risk to neotropical bees than to honeybees.	bees are key pollinators whose population numbers are declining, in part, owing to the effects of different stressors such as insecticides and fungicides. we have analysed the susceptibility of the africanized honeybee, apis mellifera, and the stingless bee, partamona helleri, to commercial formulations of the insecticides deltamethrin and imidacloprid. the toxicity of fungicides based on thiophanate-methyl and chlorothalonil were investigated individually and in combination, and with the insect ...	2017	28280585
risk assessment of pesticides and other stressors in bees: principles, data gaps and perspectives from the european food safety authority.	current approaches to risk assessment in bees do not take into account co-exposures from multiple stressors. the european food safety authority (efsa) is deploying resources and efforts to move towards a holistic risk assessment approach of multiple stressors in bees. this paper describes the general principles of pesticide risk assessment in bees, including recent developments at efsa dealing with risk assessment of single and multiple pesticide residues and biological hazards. the efsa guidanc ...	2017	28279532
pollination effects on antioxidant content of perilla frutescens seeds analysed by nmr spectroscopy.	the effects of perilla frutescens pollination on the content of seed antioxidants were analysed by agronomical and pollination trials, comparing seeds produced from bagged plants in 2013 (a) to prevent access to pollinating insects, and seeds from open-pollinated plants in 2013 (b) and 2015 (c). the seeds of open-pollinated plants were significantly more numerous and heavier than those of self-pollinated plants. (1)h nmr seed analysis showed a higher presence of phenolic compounds in open-pollin ...	2017	28278651
a pan-european epidemiological study reveals honey bee colony survival depends on beekeeper education and disease control.	reports of honey bee population decline has spurred many national efforts to understand the extent of the problem and to identify causative or associated factors. however, our collective understanding of the factors has been hampered by a lack of joined up trans-national effort. moreover, the impacts of beekeeper knowledge and beekeeping management practices have often been overlooked, despite honey bees being a managed pollinator. here, we established a standardised active monitoring network fo ...	2017	28278255
accelerated solvent extraction by using an 'in-line' clean-up approach for multiresidue analysis of pesticides in organic honey.	the worldwide loss of honeybee colonies may be due to their exposure to several contaminants (i.e., pesticides); such contamination may also have impacts on consumers' health. therefore, it is essential to develop quick and new methods to detect several pesticide residues in honey samples. in this study, the effectiveness of accelerated solvent extraction (ase) was compared with quechers methods for the analysis of 53 pesticides in organic honey by gas chromatography-triple quadrupole mass spect ...	2017	28277176
shuffling cross-validation-bee algorithm as a new descriptor selection method for retention studies of pesticides in biopartitioning micellar chromatography.	bee algorithm (ba) is an optimization algorithm inspired by the natural foraging behaviour of honey bees to find the optimal solution which can be proposed to feature selection. in this paper, shuffling cross-validation-ba (cv-ba) was applied to select the best descriptors that could describe the retention factor (log k) in the biopartitioning micellar chromatography (bmc) of 79 heterogeneous pesticides. six descriptors were obtained using ba and then the selected descriptors were applied for mo ...	2017	28277080
questionable diagnostic benefit of the commercially available panel of bee venom components.	for many years, only the major allergen rapi m 1 has been available on the immunocap system for routine diagnosis of bee venom (bv) allergy. now, there are five components available, and we aimed to detect the sensitivity and specificity of rapi m 1, 2, 3, 5, and 10 in bv-allergic patients. we further evaluated the sensitivity of rapi m 1 and 2 of an alternative platform and investigated possible differences in the sensitization profile between monosensitization and clinically relevant double se ...	2017	28273336
distribution and predictors of wing shape and size variability in three sister species of solitary bees.	morphological traits can be highly variable over time in a particular geographical area. different selective pressures shape those traits, which is crucial in evolutionary biology. among these traits, insect wing morphometry has already been widely used to describe phenotypic variability at the inter-specific level. on the contrary, fewer studies have focused on intra-specific wing morphometric variability. yet, such investigations are relevant to study potential convergences of variation that c ...	2017	28273178
cuticular hydrocarbon cues of immune-challenged workers elicit immune activation in honeybee queens.	recently, evidence has shown that variations in the cuticular hydrocarbons (chcs) profile allow healthy honeybees to identify diseased nestmates, eliciting agonistic responses in the former. here, we determined whether these 'immunologic cues' emitted by diseased nestmates were only detected by workers, who consequently took hygienic measures and excluded these individuals from the colony, or whether queens were also able to detect these cues and respond accordingly. healthy honeybee queens were ...	2017	28271576
feeding preference and sub-chronic effects of zno nanomaterials in honey bees (apis mellifera carnica).	the extensive production of zinc oxide (zno) nanomaterials (nms) may result in high environmental zinc burdens. honeybees need to have special concern due to their crucial role in pollination. our previous study indicated that low concentrations of zno nms, corresponding to 0.8 mg zn/ml, have a neurotoxic potential for honeybees after a 10-day oral exposure. present study was designed to investigate the effect of a short, dietary exposure of honeybees to zno nms at concentrations 0.8-8 mg zn/ml ...	2017	28271210
the choosing of sleeping position in the overnight aggregation by the solitary bees amegilla florea urens in iriomote island of japan.	in addition to the process of joining the sleeping aggregation, the choice of sleeping position is an important night-time behaviour of small diurnal insects because of the increased risk for predator attacks as well as bad weather. the aggregation behaviour of the solitary bee amegilla florea urens was investigated to elucidate the choice of sleeping position on substrates. male and female constructed single-sex aggregations on hanging leaves during may and june, respectively. most individuals ...	2017	28271178
sequential social experiences interact to modulate aggression but not brain gene expression in the honey bee (apis mellifera).	in highly structured societies, individuals behave flexibly and cooperatively in order to achieve a particular group-level outcome. however, even in social species, environmental inputs can have long lasting effects on individual behavior, and variable experiences can even result in consistent individual differences and constrained behavioral flexibility. despite the fact that such constraints on behavior could have implications for behavioral optimization at the social group level, few studies ...	2017	28270855
oral health of patients treated with acrylic partial dentures using a toothpaste containing bee product.	this study was carried out to investigate the influence of a propolis and tee tree oil-containing hygienic agent on selected oral health parameters, oral microflora, and the condition of periodontal health. thirty-seven patients who underwent oral rehabilitation with a removable acrylic denture were selected and randomly assigned into two groups: study group (a) which received a newly formulated propolis and tee tree oil-containing toothpaste or a control group (c) without an active ingredient. ...	2017	28265291
review of <i>marmasoma</i> white with lectotype designation of <i>m. sumptuosum</i> white and an identification key to the australasian/oceanian genera of ecliminae (diptera, bombyliidae).	the bombyliid genus marmasoma white is restricted to australia. marmasoma sumptuosum white, the only known species of the genus, is studied and a lectotype and paralectotypes are designated. based on the type series and on additional material, a redescription of the species is presented and the main characters of the external morphology of adults are illustrated and photographed, including male and female genitalia. in addition, the remarkable aedeagus of m. sumptuosum, unique among all eclimina ...	2017	28264390
taxonomic revision of the afrotropical species of <i>pachyanthidium</i> friese (hymenoptera: megachilidae: anthidiini).	the afrotropical species of pachyanthidium friese are revised. sixteen species are recognized in four subgenera. one new species, pachyanthidium (trichanthidium) anoplos sp. n. is described; and one species, pachyanthidium (trichanthidium) cucullatum (friese), is reinstated as a valid species. four new synonymy are proposed: pachyanthidium minutulum pasteels =pachyanthidium (trichanthidiodes) semiluteum pasteels syn. n.; anthidium africanum smith = p. (p.) bicolor (lepeletier) syn. n.; pachyanth ...	2017	28264276
synopsis of the bee genus <i>thygater</i> holmberg 1884 (hymenoptera, apidae) in the brazilian state of minas gerais, with the description of a new species and a key to all brazilian species.	thygater holmberg 1884, a neotropical bee genus distributed from argentina to mexico, was last revised almost 50 years ago. considering the species recognized then, and a few others described subsequently, 30 species are currently included in the genus. the brazilian state of minas gerais is a large, environmentally heterogeneous territory, including areas in the phytogeographic domains of the atlantic tropical rain forest, the semiarid caatinga and the highly seasonal cerrado. the state insect ...	2017	28264264
epigenetic modifications and their relation to caste and sex determination and adult division of labor in the stingless bee melipona scutellaris.	stingless bees of the genus melipona, have long been considered an enigmatic case among social insects for their mode of caste determination, where in addition to larval food type and quantity, the genotype also has a saying, as proposed over 50 years ago by warwick e. kerr. several attempts have since tried to test his mendelian two-loci/two-alleles segregation hypothesis, but only recently a single gene crucial for sex determination in bees was evidenced to be sex-specifically spliced and also ...	2017	28257527
the exposure of honey bees (apis mellifera; hymenoptera: apidae) to pesticides: room for improvement in research.	losses of honey bees have been repeatedly reported from many places worldwide. the widespread use of synthetic pesticides has led to concerns regarding their environmental fate and their effects on pollinators. based on a standardised review, we report the use of a wide variety of honey bee matrices and sampling methods in the scientific papers studying pesticide exposure. matrices such as beeswax and beebread were very little analysed despite their capacities for long-term pesticide storage. mo ...	2017	28256316
the effects of disturbance threat on leaf-cutting ant colonies: a laboratory study.	the flexibility of organisms to respond plastically to their environment is fundamental to their fitness and evolutionary success. social insects provide some of the most impressive examples of plasticity, with individuals exhibiting behavioral and sometimes morphological adaptations for their specific roles in the colony, such as large soldiers for nest defense. however, with the exception of the honey bee model organism, there has been little investigation of the nature and effects of environm ...	2017	28255181
characterizing the structure and oligomerization of major royal jelly protein 1 (mrjp1) by mass spectrometry and complementary biophysical tools.	royal jelly (rj) triggers the development of female honeybee larvae into queens. this effect has been attributed to the presence of major royal jelly protein 1 (mrjp1) in rj. mrjp1 isolated from royal jelly is tightly associated with apisimin, a 54-residue α-helical peptide that promotes the noncovalent assembly of mrjp1 into multimers. no high-resolution structural data are available for these complexes, and their binding stoichiometry remains uncertain. we examined mrjp1/apisimin using a range ...	2017	28252287
the darwin cure for apiculture? natural selection and managed honeybee health.	recent major losses of managed honeybee, apis mellifera, colonies at a global scale have resulted in a multitude of research efforts to identify the underlying mechanisms. numerous factors acting singly and/or in combination have been identified, ranging from pathogens, over nutrition to pesticides. however, the role of apiculture in limiting natural selection has largely been ignored. this is unfortunate, because honeybees are more exposed to environmental stressors compared to other livestock ...	2017	28250807
transfer assessment of carbendazim residues from rape flowers to apicultural products.	carbendazim is usually used to control the sclerotinia sclerotiorum of rapes during the flowering period. this paper presents a study on transfer assessment of carbendazim residues from rape flowers to apicultural products. in the field trials, the rapes were sprayed with carbendazim on standard dosage. bees produced apicultural products (bee pollen, honey, and royal jelly) from sprayed rapes. apicultural products were collected on a regular basis. carbendazim residues were extracted from bee po ...	2017	28246574
kidney injury in a dog following bee sting-associated anaphylaxis.	this report describes a case of honeybee envenomation in a dog that developed anaphylaxis after being stung by approximately 10 bees. the dog subsequently developed acute kidney injury. the dog had a previous mild increase in blood urea nitrogen with normal creatinine, possibly indicating an insidious chronic renal degenerative process that went into acute decompensation at the time of bee envenomation.	2017	28246414
floral abundance, richness, and spatial distribution drive urban garden bee communities.	in urban landscapes, gardens provide refuges for bee diversity, but conservation potential may depend on local and landscape features. foraging and population persistence of bee species, as well as overall pollinator community structure, may be supported by the abundance, richness, and spatial distribution of floral resources. floral resources strongly differ in urban gardens. using hand netting and pan traps to survey bees, we examined whether abundance, richness, and spatial distribution of fl ...	2017	28245886
volume and density of microglomeruli in the honey bee mushroom bodies do not predict performance on a foraging task.	the mushroom bodies (mbs) are insect brain regions important for sensory integration, learning, and memory. in adult worker honey bees (apis mellifera), the volume of neuropil associated with the mbs is larger in experienced foragers compared with hive bees and less experienced foragers. in addition, the characteristic synaptic structures of the calycal neuropils, the microglomeruli, are larger but present at lower density in 35-day-old foragers relative to 1-day-old workers. age- and experience ...	2017	28245532
insects, arachnids and centipedes venom: a powerful weapon against bacteria. a literature review.	currently, new antimicrobial molecules extracted or obtained by natural sources, could be a valide alternative to traditional antibiotics. most of these molecules are represented by antimicrobial peptides (amps), which are essential compounds of insect, arachnids and centipedes venom. amps, due to their strong effectiveness, low resistance rates and peculiar mode of action, seem to have all the suitable features to be a powerful weapon against several bacteria, especially considering the increas ...	2017	28242227
markov chain model-based optimal cluster heads selection for wireless sensor networks.	the longer network lifetime of wireless sensor networks (wsns) is a goal which is directly related to energy consumption. this energy consumption issue becomes more challenging when the energy load is not properly distributed in the sensing area. the hierarchal clustering architecture is the best choice for these kind of issues. in this paper, we introduce a novel clustering protocol called markov chain model-based optimal cluster heads (mochs) selection for wsns. in our proposed model, we intro ...	2017	28241492
dna methylation mediates neural processing after odor learning in the honeybee.	dna methyltransferases (dnmts) - epigenetic writers catalyzing the transfer of methyl-groups to cytosine (dna methylation) - regulate different aspects of memory formation in many animal species. in honeybees, dnmt activity is required to adjust the specificity of olfactory reward memories and bees' relearning capability. the physiological relevance of dnmt-mediated dna methylation in neural networks, however, remains unknown. here, we investigated how dnmt activity impacts neuroplasticity in th ...	2017	28240742
the buzz about bees and poverty alleviation: identifying drivers and barriers of beekeeping in sub-saharan africa.	the potential of beekeeping to mitigate the exposure of rural sub-sahara african farmers to economic stochasticity has been widely promoted by an array of development agencies. robust outcome indicators of the success of beekeeping to improve household well-being are unfortunately lacking. this study aimed to identify the key drivers and barriers of beekeeping adoption at the household level, and quantified the associated income contribution in three agro-ecological zones in uganda. beekeepers w ...	2017	28235072
repeated evolution of soldier sub-castes suggests parasitism drives social complexity in stingless bees.	the differentiation of workers into morphological castes represents an important evolutionary innovation that is thought to improve division of labor in insect societies. given the potential benefits of task-related worker differentiation, it is puzzling that physical worker castes, such as soldiers, are extremely rare in social bees and absent in wasps. following the recent discovery of soldiers in a stingless bee, we studied the occurrence of worker differentiation in 28 stingless bee species ...	2017	28232746
assessment of the toxic effect of pesticides on honey bee drone fertility using laboratory and semifield approaches: a case study of fipronil.	concern about the reproductive toxicity of plant protection products in honey bee reproducers is increasing. because the reproductive capacity of honey bees is not currently considered during the risk assessment procedure performed during plant protection product registration, it is important to provide methods to assess such potential impairments. to achieve this aim, we used 2 different approaches that involved semifield and laboratory conditions to study the impact of fipronil on drone fertil ...	2017	28224659
nadh autofluorescence, a new metabolic biomarker for cancer stem cells: identification of vitamin c and cape as natural products targeting "stemness".	here, we assembled a broad molecular "tool-kit" to interrogate the role of metabolic heterogeneity in the propagation of cancer stem-like cells (cscs). first, we subjected mcf7 cells to "metabolic fractionation" by flow cytometry, using fluorescent mitochondrial probes to detect pcg1α activity, as well ros and hydrogen-peroxide (h2o2) production; nadh levels were also monitored by auto-fluorescence. then, the various cell populations were functionally assessed for "stem cell activity", using the ...	2017	28223550
wild pollinators enhance oilseed rape yield in small-holder farming systems in china.	insect pollinators play an important role in crop pollination, but the relative contribution of wild pollinators and honey bees to pollination is currently under debate. there is virtually no information available on the strength of pollination services and the identity of pollination service providers from asian smallholder farming systems, where fields are small, and variation among fields is high. we established 18 winter oilseed rape (brassica napus l.) fields along a large geographical grad ...	2017	28222708
pancreatoprotective effects of geniotrigona thoracica stingless bee honey in streptozotocin-nicotinamide-induced male diabetic rats.	stingless bee honey (slbh) has been claimed to possess multiple health benefits. its anti-diabetic properties are however unknown. in this study, ability of slbh from geniotrigona thoracica stingless bee species in ameliorating pancreatic damage and in maintaining metabolic profiles were investigated in diabetic condition.	2017	28222394
bee venom phospholipase a2 ameliorates house dust mite extract induced atopic dermatitis like skin lesions in mice.	atopic dermatitis (ad) is a biphasic inflammatory skin disease that is provoked by epidermal barrier defects, immune dysregulation, and increased skin infections. previously, we have demonstrated that bvpla2 evoked immune tolerance by inducing regulatory t cells (treg), and thus alleviated th2 dominant allergic asthma in mice. here, we would like to determine whether treatment with bvpla2 exacerbates the ad-like allergic inflammations induced by house dust mite extract (dfe) in a murine model. e ...	2017	28218721
the importance of pollen chemistry in evolutionary host shifts of bees.	although bee-plant associations are generally maintained through speciation processes, host shifts have occurred during evolution. understanding shifts between both phylogenetically and morphologically unrelated plants (i.e., host-saltation) is especially important since they could have been key processes in the origin and radiation of bees. probably far from being a random process, such host-saltation might be driven by hidden constraints associated with plant traits. we selected two clades of ...	2017	28216663
seasonal cycle of inbreeding and recombination of the parasitic mite varroa destructor in honeybee colonies and its implications for the selection of acaricide resistance.	varroa destructor is the most devastating parasite of the western honeybee, apis mellifera. in the light of the arm race opposing the host and its parasite, the population dynamics and genetic diversity of these organisms are key parameters. however, the life cycle of v. destructor is characterized by extreme inbreeding due to full sibling mating in the host brood cells. we here present an equation reflecting the evolution of inbreeding in such a clonal system, and compare our predictions with e ...	2017	28216419
nesting habits influence population genetic structure of a bee living in anthropogenic disturbance.	while most organisms are negatively affected by anthropogenic disturbance, a few species thrive in landscapes altered by humans. typically, native bees are negatively impacted by anthropogenic environmental change, including habitat alteration and climate change. here, we investigate the population structure of the eastern carpenter bee xylocopa virginica, a generalist pollinator with a broad geographic range spanning eastern north america. eastern carpenter bees now nest almost exclusively in a ...	2017	28214357
regional differences in the preferred e-vector orientation of honeybee ocellar photoreceptors.	in addition to compound eyes, honeybees (apis mellifera) possess three single lens eyes called ocelli located on the top of the head. ocelli are involved in head-attitude control and in some insects have been shown to provide celestial compass information. anatomical and early electrophysiological studies have suggested that uv and blue-green photoreceptors in ocelli are polarization sensitive. however, their retinal distribution and receptor characteristics have not been documented. here, we us ...	2017	28213397
multiple pesticide residues in live and poisoned honeybees - preliminary exposure assessment.	study combines data about the exposure of honeybees to pesticides from plant protection products and veterinary medicinal products. residues of 200 pesticide and pesticide metabolites in 343 live and 74 poisoned honeybee samples, obtained during the years of 2014-2015, were determined by lc-ms/ms and gc-ms/ms. in 44% of live honeybee 48 different pesticide residues were found, mainly amitraz metabolites (dmf, dmpf) and chlorpyrifos. in 98% of poisoned honeybee 57 pesticides and metabolites were ...	2017	28211333
simultaneous determination of absolute configuration and quantity of lipopeptides using chiral liquid chromatography/mass spectrometry and diastereomeric internal standards.	lipopeptides promote innate immune response and are related to disease pathology. to investigate the newly emerging roles of lipopeptides, accurate measurements of stereoisomers with multiple chiral centers are essential yet challenging. this work uses (3r)- and (3s)-(15-methyl-3-((13-methyltetradecanoyl)oxy)hexadecanoyl)glycyl-l-serine, abbreviated as l-serine-(r+s)-lipid 654, to develop a method that combines chiral liquid chromatography, a diastereomeric mixture of isotopically labeled intern ...	2017	28207237
immune system stimulation by the gut symbiont frischella perrara in the honey bee (apis mellifera).	gut bacteria engage in various symbiotic interactions with their host and impact gut immunity and homeostasis in different ways. in honey bees, the gut microbiota is composed of a relatively simple, but highly specialized bacterial community. one of its members, the gammaproteobacterium frischella perrara induces the so-called scab phenotype, a dark-coloured band that develops on the epithelial surface of the pylorus. to understand the underlying host response, we analysed transcriptome changes ...	2017	28207182
using dna metabarcoding to investigate honey bee foraging reveals limited flower use despite high floral availability.	understanding which flowers honey bees (apis mellifera) use for forage can help us to provide suitable plants for healthy honey bee colonies. accordingly, honey dna metabarcoding provides a valuable tool for investigating pollen and nectar collection. we investigated early season (april and may) floral choice by honey bees provided with a very high diversity of flowering plants within the national botanic garden of wales. there was a close correspondence between the phenology of flowering and th ...	2017	28205632
melittin inhibits osteoclast formation through the downregulation of the rankl-rank signaling pathway and the inhibition of interleukin-1β in murine macrophages.	melittin is a major toxic component of bee venom (apis mellifera). it is not known whether melittin is involved in bone metabolism and osteoclastogenesis. the aim of this study was to determine the role of melittin in the regulation of osteoclastogenesis. in vitro osteoclastogenesis assays were performed using mouse raw 264.7 cells and bone marrow-derived macrophages (bmms) treated with receptor activator of nuclear factor-κb ligand (rankl) and macrophage colony-stimulating factor (m-csf). morph ...	2017	28204822
migratory bee hive transportation contributes insignificantly to transgenic pollen movement between spatially isolated alfalfa seed fields.		2017	28204486