Publications

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optimization and validation of a liquid chromatography tandem mass spectrometry (lc/msn) method for analysis of corticosteroids in bovine liver: evaluation of keyhole limpet beta-glucuronidase/sulfatase enzyme extract.a liquid chromatography tandem mass spectrometry (lc/ms(n)) method for the determination of 12 corticosteroids in bovine liver has been optimized and validated in accordance with the european commission decision 2002/657/ec. a bovine liver sample was deconjugated with beta-glucuronidase/sulfatase enzyme, extracted with diethyl ether and further cleaned up with solid phase extraction (spe) before analysis with lc/ms(n). two different enzyme extracts (originating from helix pomatia and keyhole lim ...200919211313
the immunological effects of electrolyzed reduced water on the echinostoma hortense infection in c57bl/6 mice.electrolyzed reduced water (erw) is widely used for drinking by people in asia. the purpose of this study was to examine the immunological effect of erw on the immunity of animals by supplying erw to c57bl/6 mice infected with echinostoma hortense metacercariae. in the non-infected groups, interleukin (il)-4 (p < 0.001), il-5, il-10, il-1beta, tumor necrosis factor (tnf)-alpha and immunoglobulin (ig) a expression of the group fed erw (erw group) increased in small intestine compared with the nor ...200919252295
acetylcholine inhibits nitric oxide (no) synthesis in the gastropod nervous system.acetylcholine (ach) is one of the main signals regulating nitric oxide synthase (nos) expression and nitric oxide (no) biosynthesis in mammals. however, few comparative studies have been performed on the role of ach on nos activity in non-mammalian animals. we have therefore studied the cholinergic control of nos in the snail helix pomatia and compared the effects of ach on no synthesis in the enteric nervous system of the snail and rat. analyses by the nadph-diaphorase reaction, immunocytochemi ...200919255787
identification and characterization of major proteins carrying abo blood group antigens in the human kidney.it is generally admitted that abo(h) blood group antigens are linked to lipids and proteins. although glycolipids carrying abo antigens have been well characterized in human kidneys, glycoproteins carrying abo antigens are largely unknown, and their molecular properties remain to be elucidated.200919384157
lectin mapping reveals stage-specific display of surface carbohydrates in in vitro and haemolymph-derived cells of the entomopathogenic fungus beauveria bassiana.the entomopathogenic fungus beauveria bassiana and its insect host target represent a model system with which to examine host-pathogen interactions. carbohydrate epitopes on the surfaces of fungal cells play diverse roles in processes that include adhesion, non-self recognition and immune invasion with respect to invertebrate hosts. b. bassiana produces a number of distinct cell types that include aerial conidia, submerged conidia, blastospores and haemolymph-derived cells termed in vivo blastos ...200919608611
tumor-specific protein human galectin-1 interacts with anticancer agents.the present work shows a novel binding activity of the tumor specific lectin--recombinant human galectin-1 (hgal-1)--to three porphyrin compounds: (1) zn-porphyrin (zntpps); (2) mn-porphyrin and (3) au-porphyrin. these compounds are widely applied in the photodynamic therapy of cancer (pdt). our data indicate that hgal-1, similar to some plant lectins, a bacterial lectin from pseudomonas aeruginosa and an animal lectin from helix pomatia, possesses dual functions binding to both carbohydrate and ...200919823749
decreasing prevalence of herpes simplex virus-2 antibodies in selected groups of women in sweden.high estimates of herpes simplex virus-2 (hsv-2) seroprevalence have been reported for women who were pregnant in sweden in the 1980s, probably reflecting sexual risk-taking during the 1960s and 1970s. the aim of the present study was to evaluate the hsv-2 seroprevalence in pregnant women and in the female attendees at a clinic for sexually transmitted infections in sweden at the beginning of the 21st century and to compare the results with those of earlier swedish studies. sera were collected d ...200919997695
characterization and role of helix contactin-related proteins in cultured helix pomatia neurons.we report on the structural and functional properties of the helix contactin-related proteins (hcrps), a family of closely related glycoproteins previously identified in the nervous system of the land snail helix pomatia through antibodies against the mouse f3/contactin glycoprotein. we focus on hcrp1 and hcrp2, soluble fniii domains-containing proteins of 90 and 45 kd bearing consensus motifs for both n- and o-glycosylation. using the anti-hcrps serum, we find secreted hcrps in helix nervous ti ...200918756517
purification of cadmium-binding proteins from related species of terrestrial helicidae (gastropoda, mollusca): a comparative study.three species of terrestrial helicidae (helix pomatia, cepaea hortensis and arianta arbustorum) were fed cadmium-rich diet in the laboratory. the snails accumulated high amounts of the metal in their hepatopancreas. most cadmium and some zinc were found, after centrifugation, in the soluble fractions from which a cadmium-binding protein was isolated for each species by ion exchange and gel chromatography. the proteins contained different amounts of cadmium, but little or no zinc, and showed high ...20092725484
an allelic difference determines reciprocal patterns of expression of binding sites for dolichos biflorus lectin in inbred strains of mice.we used staining of tissue sections by lectin conjugates to screen inbred strains of mice for polymorphisms which could be used as histological markers of chimaerism. we found one polymorphism, which involves reciprocal patterns of expression of binding sites for the n-acetyl-galactosamine-binding lectins from dolichos biflorus (dba), helix pomatia (hpa) and wisteria floribunda (wfa) on intestinal epithelium and vascular endothelium. the polymorphism is due to alleles at a single locus, designat ...20094031755
comparison of sample preparation methods for analysis of isoflavones in foodstuffs.due to the lack of one universally applicable and commonly used reference method, sample preparation in isoflavone (if) analysis has been performed by many different methods which renders comparison and quality assessment of published if contents in foodstuffs difficult. in the present work, the impact of different experimental parameters on the if concentrations determined in soybeans, tofu, soy drink and textured vegetable protein by different extraction and hydrolysis methods was assessed and ...200919166724
[monoclonal antibodies to individual antigens of the nervous system of the snail, helix pomatia].seven positive hybridoma clones were chosen by immunoenzyme analysis amons 103 clones obtained by hybridization of nso plasmocytoma cells and splenocytes from balb/c mice, immunized with snail's nervous system antigens. specific binding of mabs with neuron cytoplasmic antigens was indicated on cryostat sections of visceral, pedal and cerebral ganglia. the mabs obtained could be used for the study of physiological role of antigens identified.20082268718
[the effect of high energy irradiation on the membrane potential and impulse activity of neurons in the brain of helix pomatia].in electrophysiological experiments with a preparation of the isolated helix pomatia brain, a study was made of the effect of pulsed irradiation with high-energy electrons (20 mev) on membrane potentials and pulse activity of "silent", pacemaking and postsynaptic neurons. it was shown that after irradiation with 150 and 300 gy (dose rate 5 gy/s and pulse frequency 50 hz) "silent" neurons retain their excitability. pacemaking neurons responded to radiation by a drastic increase in spontaneous pul ...20082587699
[the effect of calcium ions on the deactivation of the calcium current in nerve cells of helix pomatia]. 20082546725
comparison of the carbohydrate-binding specificities of seven n-acetyl-d-galactosamine-recognizing lectins.seven plant lectins, dolichos biflorus agglutinin (dba), griffonia simplicifolia agglutinin (gsa, isolectin a4), helix pomatia agglutinin (hpa), soybean (glycine max) agglutinin (sba), salvia sclarea agglutinin (ssa), vicia villosa agglutinin (vva, isolectin b4) and wistaria floribunda agglutinin (wfa), known to be specific for n-acetyl-d-galactosamine-(galnac) bearing glycoconjugates, have been compared by the binding of their radiolabelled derivatives, to eight well-characterized synthetic oli ...20082384093
characterization of nitric oxidergic neurons in the alimentary tract of the snail helix pomatia l.: histochemical and physiological study.by using nadph-diaphorase (nadph-d) histochemistry, nitric oxide synthase (nos) immunohistochemistry, western blotting, and no pharmacology, we investigated the distribution and possible function of nos-containing neurons in different units of the alimentary tract of the snail, helix pomatia. discrete populations of neurons in the buccal ganglia displayed nadph-d reactivity. nadph-d-reactive and nos-immunoreactive (nos-ir) neurons were present in the caecum, and labeled fibers were found to inne ...200818076086
lectin-binding properties of aeromonas caviae strains.the cell surface carbohydrates of four strains of aeromonas caviae were analyzed by agglutination and lectin-binding assays employing twenty highly purified lectins encompassing all sugar specificities. with the exception of l-fucose and sialic acid, the sugar residues were detected in a. caviae strains. a marked difference, however, in the pattern of cell surface carbohydrates in different a. caviae isolates was observed. specific receptors for tritricum vulgaris (wga), lycopersicon esculentum ...200824031204
structural organization of the sensory systems of the snail.the author's data on the structural organization of the nervous system of the body wall, the gravitational organs (the statocysts, the eyes, and the chemosensory organs), and the head tentacles of the snails helix vulgaris and helix pomatia are correlated in this paper. the localization in the cns and some structural characteristics of the central divisions of the sensory systems are shown. information is presented on the structure of the procerebrum, an associative center participating in the p ...20088208381
[the structural organization of the snail sensory systems].in the paper are reviewed the author's data on the structure of peripheral nervous system of the body wall, gravitational organs--statocysts, eyes, and organs of distant chemoreception--ommatophors, or posterior head tentacles, of the snails helix vulgaris and helix pomatia. localization in the cns of central parts of the main sensory systems is shown and some structural characteristics of them are described. the data on the organization of the procerebrum which takes part in processing of infor ...20081284183
quantification of 19-nortestosterone sulphate and boldenone sulphate in urine from male horses using liquid chromatography/tandem mass spectrometry.following administration of the anabolic steroid 19-nortestosterone or its esters to the horse, a major urinary metabolite is 19-nortestosterone-17beta-sulphate. the detection of 19-nortestosterone in urine from untreated animals has led to it being considered a naturally occurring steroid in the male horse. recently, we have demonstrated that the majority of the 19-nortestosterone found in extracts of 'normal' urine from male horses arises as an artefact through decarboxylation of the 19-carbox ...200818777513
phenotypic and genotypic characterisation of blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential.despite frequent reports on the presence of blastocystis hominis in human intestinal tract, its pathogenicity remains a matter of intense debate. these discrepancies may be due to the varying pathogenic potential or virulence of the isolates studied. the present study represents the first to investigate both phenotypic and genotypic characteristics of b. hominis obtained from symptomatic and asymptomatic individuals. symptomatic isolates had a significantly greater size range and lower growth ra ...200818795333
o-diphenol oxidase activity of molluscan hemocyanins.diphenoloxidase activities of two molluscan hemocyanins, isolated from the marine snails rapana venosa and garden snails helix vulgaris were studied using o-diphenol and l-dopa as substrates. the dimers of h. vulgaris hc show both, diphenol (k(m)=2.86 mm and k(cat)=4.48) and l-dopa activity due to a more open active sites of the enzyme and better access of the substrates. the k(m) value of molluscan h. vulgaris hc is very close to those of helix pomatia and sepia officinalis hcs, but several tim ...200818162195
pharmacokinetics and metabolism of benzophenone 2 in the rat.twelve derivatives of benzophenone (bp1-bp12) are widely used as uv-screens to protect industrial products from light induced damage. there is growing public concern about industrially produced chemicals that might interfere with hormonal signalling pathways, thus having potential adverse effects on human health. the derivative 2,2',4,4'-tetrahydroxybenzophenone (bp2) which is used in cosmetic products and in packaging materials, was previously shown to be an estrogenic endocrine active chemical ...200818242814
optimization of solid phase extraction clean up and validation of quantitative determination of corticosteroids in urine by liquid chromatography-tandem mass spectrometry.a solid phase extraction (spe) method for extraction and clean up of 9 synthetic corticosteroids was optimized for quantification by reversed-phase high-performance liquid chromatography/negative electrospray ionisation mass spectrometry. clean up was accomplished using a mixed mode polymeric strong anion exchange spe column. the final method was validated according to eu regulations for determination of residues of veterinarian drugs in products of animal origin. initial results showed a large ...200818486661
the presence and distribution of pituitary adenylate cyclase activating polypeptide and its receptor in the snail helix pomatia.the aim of this study was to show the presence, distribution and function of the pituitary adenylate cyclase activating polypeptide (pacap) and its receptors in the cns and peripheral nervous system of the mollusk, helix pomatia. pacap-like and pituitary adenylate cyclase activating polypeptide receptor (pac1-r)-like immunoreactivity was abundant both in the cns and the peripheral nervous system of the snail. in addition several non-neuronal cells also revealed pacap-like immunoreactivity. in in ...200818590802
measuring urinary testosterone levels of the great apes--problems with enzymatic hydrolysis using helix pomatia juice.helix pomatia (hp) juice is a common enzymatic preparation for deconjugation of urinary steroids. it has been used in many published studies on urinary testosterone (t) in chimpanzees and bonobos, although the ability of hp juice to convert other urinary steroids into t has been reported for human urine. we developed a protocol for determination of reliable t levels in primate urine using liquid chromatography-mass spectrometry. t levels were determined in a set of human, bonobo and chimpanzee u ...200818639242
the c-type lectin macrophage galactose-type lectin impedes migration of immature apcs.dendritic cells (dcs) are the most potent apcs of the immune system that seed the peripheral tissues and lymphoid organs. in an immature state, dcs sample their surroundings for incoming pathogens. upon ag encounter, dcs mature and migrate to the lymph node to induce adaptive immune responses. the c-type macrophage galactose-type lectin (mgl), expressed in immature dcs, mediates binding to glycoproteins carrying galnac moieties. in the present study, we demonstrate that mgl ligands are present o ...200818713985
f3/contactin-related proteins in helix pomatia nervous tissue (hcrps): distribution and function in neurite growth and neurotransmitter release.by using antibodies against mouse f3/contactin, we found immunologically related glycoproteins expressed in the nervous tissue of the snail helix pomatia. helix contactin-related proteins (hcrps) include different molecules ranging in size from 90 to 240 kd. clones isolated from a cdna expression library allowed us to demonstrate that these proteins are translated from a unique 6.3-kb mrna, suggesting that their heterogeneity depends on posttranslational processing. this is supported by the resu ...200817941055
proteome analysis of metastatic colorectal cancer cells recognized by the lectin helix pomatia agglutinin (hpa).the lectin from helix pomatia (hpa) binds to adenocarcinomas with a metastatic phenotype but the glycoconjugates of cancer cells that bind to the lectin have yet to be characterized in detail. we used a model of metastatic (ht29) and nonmetastatic (sw480) human colorectal cancer cells and a proteomic approach to identify hpa binding glycoproteins. cell membrane proteins purified by hpa affinity chromatography, were separated by 2-de and analyzed by ms. competitive inhibition experiments with n-a ...200717994629
the c-type lectin mgl expressed by dendritic cells detects glycan changes on muc1 in colon carcinoma.the epithelial mucin muc1 is a high molecular weight membrane glycoprotein frequently overexpressed and aberrantly glycosylated in adenocarcinoma. mucins normally contain high amounts of o-linked carbohydrate structures that may influence immune reactions to this antigen. during malignant transformation, certain glyco-epitopes of muc1, such as tn-antigen, tf-antigen and their sialylated forms become exposed. the role of these glycan structures in tumor biology is unknown, but their presence is k ...200717195076
stable expression of cryptosporidium parvum glycoprotein gp40/15 in toxoplasma gondii.cryptosporidium is a cause of diarrheal disease worldwide. parasite glycoproteins involved in invasion of cryptosporidium into host cells have been investigated as possible targets for effective interventions against this parasite. one of these, cpgp40/15, is expressed as a precursor protein that is cleaved by a parasite-derived furin-like protease activity into gp15, a glycophosphatidyl inositol anchored surface protein, and gp40, that associates with gp15 and binds to host cells. investigation ...200717275106
reactivities of n-acetylgalactosamine-specific lectins with human iga1 proteins.lectins are proteins with specificity of binding to certain monosaccharides or oligosaccharides. they can detect abnormal glycosylation patterns on immunoglobulins in patients with various chronic inflammatory diseases, including rheumatoid arthritis and iga nephropathy (igan). however, lectins exhibit binding heterogeneity, depending on their source and methods of isolation. to characterize potential differences in recognition of terminal n-acetylgalactosamine (galnac) on iga1, we evaluated the ...200717275907
[activation of cardiac contractivity of the edible snail h. pomatia under effect of thrombin. study of role of camp].thrombin acts on mammalian cells through specific, the so-called protease-activated receptors (pars). the thrombin action is mediated via three out of four known types of these receptors par(1, 3, 4). mammalian thrombin receptors, apart from performance of other functions, control cardiac and vascular contractility. it is not known whether receptors of such kind exist in invertebrate animals. in the present work we have showed for the first time that thrombin in the concentration range of 0.01-1 ...200717408090
detection of glucocorticoid residues in pig liver by high-performance liquid chromatography with on-line electrogenerated [cu(hio6)2](5-)--luminol chemiluminescence detection.a novel method was developed for the simultaneous determination of glucocorticoid residues such as triamcinolone (tr), prednisolone (pr), hydrocortisone (hc), cortisone (co), methylprednisolone (mp), dexamethasone (de) and triamcinolone acetonide (ta) by high-performance liquid chromatography (hplc) coupled with chemiluminescence (cl) detection. the procedure was based on the enhancement effect of glucocorticoids on the chemiluminescence reaction between luminol and the complex of trivalent copp ...200717449041
tandem exploitation of helix pomatia glycosyltransferases: facile syntheses of h-antigen-bearing oligosaccharides.snails from the family helicidae produce in their albumen glands a highly branched galactan, which consists almost exclusively of d- and l-galactose. the d-gal residues are glycosydically beta(1-->6)- or beta(1-->3)-linked, whereas the l-gal moieties are attached alpha(1-->2). up until the present time, two beta(1-->6)-d-galactosyl transferases and one alpha(1-->2)-l-galactosyl transferase have been identified in a membrane preparation of these glands. these were used to synthesise various oligo ...200717591726
isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, helixpomatia and cornu aspersum (gastropoda: pulmonata).the intestinal microbiota of the edible snails cornu aspersum fsyn: h. aspersa), and helix pomatia were investigated by culture-based methods, 16s rrna sequence analyses and phenotypic characterisations. the study was carried out on aestivating snails and two populations of h. pomatia were considered. the cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) cfu g -1, but the swedish h. pomatia appeared significantly less colonised, suggesting a higher sensitivity ...200717657348
neurohypophysial peptides selectively depressed high voltage-activated ca-current in snail neurons.it was found that neurohypophysial peptides oxytocin and lys-vasopressin selectively decreased the high voltage-activated component of the inward ca-current in voltage-clamped helix pomatia identified neurons in a dose- and time-dependent manner. this effect was unaffected or further enhanced applying phosphodiesterases inhibitors. it is suggested that suppression of high voltage-activated current was due to the activation of the adenylate cyclase system, likely protein kinase a.20071963481
[a neurophysiological analysis of the response pattern to direct electrical stimulation of the lpa3 and rpa3 neurons in the edible snail]. 20072169170
recognition of beta-glucuronidase by the calcium-independent phosphomannosyl surface receptor of haemocytes from the gastropod mollusc, helix pomatia. 20061323490
17 alpha,20 beta-dihydroxy-4-pregnen-3-one 20-sulphate: a major new metabolite of the teleost oocyte maturation-inducing steroid.this paper describes the discovery of 17 alpha,20 beta-dihydroxy-4-pregnen-3-one 20-sulphate (17,20 beta-p-sulphate) in urine of male and female plaice pleuronectes platessa, in female atlantic salmon salmo salar and in female dover sole solea solea. 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-p) induces oocyte final maturation in teleosts and, whereas levels of the free steroid in maturing/ovulating female plaice are generally less than 1 ng ml-1 and poorly associated with the stage ...20061563623
diabetes mellitus associated changes in glomerular glycocompounds: a fluorescence microscopical study.renal biopsy specimens showing histological alterations typical of advanced diabetic glomerulopathy were studied for changes in glomerular glycoconjugates, using fluorochrome-coupled lectins as probes. all samples studied showed a marked reduction in the binding of triticum vulgaris (wga) lectin in the glomerular basement membranes. on the other hand, 'new' glomerular binding sites for the lectins of dolichos biflorus (dba), helix pomatia (hpa) and arachis hypogaea (pna), recognizing galactosyl ...20063667339
purification of the crude solution from helix pomatia for use as beta-glucuronidase and aryl sulfatase in phytoestrogen assays.phytoestrogens occur in a variety of foods and are thought to offer a protective effect against a number of complex diseases. due to the diversity of phytoestrogen conjugates formed in the human body, most assays include an enzymatic hydrolysis step prior to analysis. beta-glucuronidase from helix pomatia, which also contains sulfatase activity, is popular for this task but contains appreciable levels of some phytoestrogens and related compounds, which could affect accurate quantification at low ...200616388994
in vivo xenograft models of breast cancer metastasis.if breast cancer patients are not cured, it is largely because of the fact that the cancer has spread beyond its primary site--the breast--to distant sites, such as, e.g., bone marrow, lung, brain, and/or liver. these secondary tumors are called metastases, and the underlying mechanisms leading to these secondary tumor deposits are complex and still ill understood. in this chapter, we report on how to develop clinically relevant human breast cancer cell line xenografts in severe combined immunod ...200616491620
the effect of lectins on cryptosporidium parvum oocyst in vitro attachment to host cells.the influence of lectins on cryptosporidium parvum oocyst agglutination and on attachment to both fixed madin darby canine kidney (mdck) cells and fixed hct-8 (human colorectal epithelial) cells was examined. oocyst cell wall characteristics were examined by transmission electron microscopy. lectin-free oocysts were shown to adhere equally to both mdck cells and hct-8 cells. in mdck cells, the addition of 1-25 microg/ml codium fragile lectin, 10 microg/ml maclura pomifera lectin, 10 microg/ml he ...200616629306
alternative methodology for the analysis of progesterone, testosterone, and epi-testosterone in bovine liver and veal muscle.research has shown that traditional solvent extraction procedures used for the analysis of endogenous steroids often give inconsistent recoveries and results. however, a single-laboratory validation of a liquid chromatography/tandem mass specrometry method using 2 product ions per transition for progesterone, testosterone, and epi-testosterone in bovine liver and veal muscle showed accuracy and precision to within 23% at concentrations ranging from 0.5 to 2.0 microg/kg. homogenized samples were ...200616640309
biochemical and structural analysis of helix pomatia agglutinin. a hexameric lectin with a novel fold.helix pomatia agglutinin (hpa) is a n-acetylgalactosamine (galnac) binding lectin found in the albumen gland of the roman snail. as a constituent of perivitelline fluid, hpa protects fertilized eggs from bacteria and is part of the innate immunity system of the snail. the peptide sequence deduced from gene cloning demonstrates that hpa belongs to a family of carbohydrate-binding proteins recently identified in several invertebrates. this domain is also present in discoidin from the slime mold di ...200616704980
[comparative analysis of map/erk-kinase activation in the cns of animals with different capability for learning].with the western-blot analysis, we studied the activation of map/erk protein kinases (regulating genes expression by the phosphorylation of transcription factors (tf) creb and elk-1) in native helix pomatia and in animals with disturbed abilities to form long-term conditioned avoidance reflexes. different stages of this reflex formation were shown to be characterized by different levels of map/erk kinases activation, the latter being different in cns's regions (visceral complex of ganglia, cereb ...200616869282
functional interaction of escherichia coli heat-labile enterotoxin with blood group a-active glycoconjugates from differentiated ht29 cells.human colon adenocarcinoma cells (ht29-atcc) and the clone ht29-5f7 were cultured under conditions that differentiate cells to a polarized intestinal phenotype. differentiated cells showed the presence of junctional complexes and intercellular lumina bordered by microvilli. intestinal brush border hydrolase activities (sucrase, aminopeptidase n, lactase and maltase) were detected mainly in differentiated ht29-atcc cells compared with the differentiated clone, ht29-5f7. the presence of non-gm1 re ...200616884490
an accurate and reproducible method for the quantitative analysis of isoflavones and their metabolites in rat plasma using liquid chromatography/mass spectrometry combined with photodiode array detection.to study the safety and potential health benefits of soy isoflavones, a rapid and simple method based on liquid chromatography combined with mass spectrometry (lc/ms) and photodiode array detector (pda) was developed for the determination of isoflavones in rat plasma. the analytes included daidzein, genistein, glycitein, equol, 4-ethyl phenol, and biochanin a over a concentration range of 1.0-4320.0 nm using 75 microl of rat plasma. rat plasma samples were hydrolyzed by adding an enzyme mixture ...200616915859
structure and in vitro cytocompatibility of the gastropod shell of helix pomatia.distinguishing features of biological constructions are high stability and adaptation to their environment. beside biocompatibility, nontoxicity and degradability these characteristics are demanded for new biomaterials in the field of tissue engineering. this study investigated the chemical composition, the organization and the in vitro osteoconductive potential of the terrestrial gastropod shell (helix pomatia) on cal72 and human osteoblast-like cells. chemical composition of the biomaterial wa ...200617010402
assessing the instability of the isoelectric focusing patterns of erythropoietin in urine.ief can be used to differentiate human urinary erythropoietin (uepo), recombinant human erythropoietin or epoetin (repo) and darbepoetin (novel erythropoiesis stimulating protein (nesp)). this is the basis of the method currently used to detect misuse of repo and nesp by elite athletes. recently, an unknown activity has been attributed to some urine samples (denominated 'unstable' urine by the world anti-doping agency; wada). this activity has shown to give rise to artefactual profiles for both ...200617054082
glycoproteome profiling of transforming growth factor-beta (tgfbeta) signaling: nonglycosylated cell death-inducing dff-like effector a inhibits tgfbeta1-dependent apoptosis.transforming growth factor-beta (tgfbeta) is a potent regulator of cell growth, differentiation, and apoptosis. tgfbeta binds to specific serine/threonine kinase receptors, which leads to activation of smad-dependent and smad-independent signaling pathways. o-glycosylation is a dynamic ptm which has been observed in many regulatory proteins, but has not been studied in the context of tgfbeta signaling. to explore the effect of tgfbeta1 on protein o-glycosylation in human breast epithelial cells, ...200617080483
selection and optimization of hydrolysis conditions for the quantification of urinary metabolites of mdma.recovery of 3,4-methylenedioxymethamphetamine (mdma) urinary metabolites requires optimization of the hydrolysis of 4-hydroxy-3-methyoxymethamphetamine (hmma), 4-hydroxy-3-methoxyamphetamine (hma), and 3,4-methylenedioxyamphetamine (mda) conjugates prior to chromatographic analysis. acidic and enzymatic hydrolysis with beta-glucuronidase from escherichia coli and helix pomatia were evaluated. acid hydrolysis yielded 40.0% and 39.3% higher hma recovery compared to e. coli and h. pomatia hydrolysi ...200617132253
systematic analysis of animals used in medieval azerbaijan medicine.in order to study the special composition of animals used in the medieval medicine of azerbaijan, a wide range of medieval sources on medicine and pharmacognosy from the collection of the institute of manuscripts of the azerbaijan academy of sciences in baku has been studied. about 40 medieval sources from the 10-18th centuries including 17 manuscripts in turkic, persian and arabic have been selected as the objects of this study. as a result, 150 species of animals described in medieval azerbaij ...200617153727
detection of synthetic glucocorticoid residues in cattle tissue and hair samples after a single dose administration using lc-ms/ms.a sensitive and specific liquid chromatography-tandem mass spectrometry (lc-ms/ms) assay for the detection of several synthetic glucocorticoids in kidney, muscle and hair samples of cattle after a single intramuscular injection is described. after a dichloromethane wash of the hair samples, analytes were released from the hair matrix by enzymatic digestion. muscle samples were also digested enzymatically using proteinase, while kidney samples were deconjugated by helix pomatia juice. these preli ...200515686988
cancer metastasis: characterization and identification of the behavior of metastatic tumor cells and the cell adhesion molecules, including carbohydrates.this review focuses on the behavior of metastatic tumor cells and their specific adhesion molecules. much of this review is based on the results from our researches over many years. electron microscopic investigations of metastatic processes have demonstrated that desmosomes, tight junctions, or cell fusion-like structures are formed between tumor cells and other cells such as endothelial, mesothelial, hepatic, and nerve cells. these findings suggest that metastatic tumor cells acquire specific ...200515720223
enrichment of cheeses manufactured from cow's and sheep's milk blends with sheep-like species-related alkylphenols.enhancement of concentrations of species-related sheep-like alkylphenols, p- and m-cresols and 3- and 4-ethylphenols, in experimental manchego-type cheeses manufactured from cow's and sheep's milk blends (80:20) by using arylsulfatases was investigated. a food-grade arylsulfatase from aspergillus oryzae (atcc 20719) was produced using a stimulatory medium, and crude dried cells were used as the enzyme source. exogenous arylsulfatases from helix pomatia and a. oryzae were added to cheese curd, an ...200515740062
the tidemark of the chondro-osseous junction of the normal human knee joint.the chondro-osseous junction includes the junction between calcified and non-calcified cartilage matrices often referred to as the tidemark. a detailed knowledge of the structure, function and pathophysiology of the chondro-osseous junction is essential for an understanding both of the normal elongation of bones and of the pathogenesis of osteoarthrosis. in this study the molecular anatomy of the tidemark was studied using histochemical techniques, including lectin histochemistry, on blocks of n ...200515900412
optimization of conditions for the enzymatic hydrolysis of phytoestrogen conjugates in urine and plasma.optimal ph, temperature, and concentration of enzyme conditions for the rate of hydrolysis of five isoflavone conjugates (daidzein, o-desmethylangolensin, equol, genistein, and glycitein) and two lignans (enterodiol and enterolactone) from two biological matrices (urine and plasma) were studied using beta-glucuronidase from helix pomatia. in addition, the use of mixtures of beta-glucuronidase and sulfatase enzymes from different sources was investigated to find enzyme preparations that contained ...200515907867
gas chromatograph-mass spectrometric method for the determination of carvedilol and its metabolites in human urine.a sensitive and efficient method was developed for the determination of carvedilol and its metabolites in human urine by gas chromatography-mass spectrometry (gc-ms). urine samples were hydrolyzed with beta-glucuronidase/arylsulfatase (from helix pomatia) and the target compounds were extracted with liquid-liquid extraction. the extracts were completely derivatized with mstfa and mbtfa and analyzed by gc-ms using an ultra-2 column. the linearity of the assay ranges were 0.75-75 ngml(-1) for carv ...200515996536
the lectin helix pomatia agglutinin as a marker of metastases--clinical and experimental studies.the prognosis for the majority of patients suffering from a solid neoplasm remains bleak, due to the fact that most cancers have already formed metastases at distant sites. any progress in cancer therapy will, therefore, depend on the understanding of the metastatic cascade and therapies derived from this understanding. the development of clinically relevant models of metastases, using lectin-defined human cancer cell lines and severe combined immunodeficient (scid) mice, is described. in conjun ...200516033108
hydrolysis of conjugated metabolites of buprenorphine ii. the quantitative enzymatic hydrolysis of norbuprenorphine-3-beta-d-glucuronide in human urine.in gas chromatographic-mass spectroscopic analysis of buprenorphine metabolites, the urine specimen must be first hydrolyzed to release buprenorphine and norbuprenorphine from their glucuronide conjugates. for evaluation of existing hydrolysis methods and to find out the optimal hydrolysis conditions, buprenorphine-3-beta-d-glucuronide (b3g) and norbuprenorphine-3-beta-d-glucuronide (nb3g) were synthesized. in a previous communication we reported on the optimum conditions for hydrolysis of b3g. ...200516168181
membrane mechanisms of antiarrhythmic effect of quaternidine.complex electrophysiological study of the effects of quaternidine carried out on intact hearts from cats, myocardial fragments from rats, and single ionic channels of large edible snail showed that quaternidine demonstrates properties of class 1b antiarrhythmic drug according to vaughan-williams nomenclature. this agent did not suppress nomotopic pacemaker automaticity, did not change conduction in ventricles, atria, and atrioventricular junction in hearts with preserved sinus rhythm, did not pr ...200516224582
evidence for the cd(2+) activation of the aryl sulfatase from helix pomatia.often used to remove sulfate groups from carbohydrates, the regulatory properties of the aryl sulfatase from helix pomatia remain little characterized. as many hydrolytic enzymes utilize exogenous metal ions in catalysis, the effect of various divalent metal ions on the sulfatase was investigated. evidence for metal ion activation was collected, with cd(2+) being notable for effective activation. the enzyme was inhibited by cu(2+). the response of other common hydrolases to divalent metal ions w ...200516333754
isolation and characterization of metabolically competent pulmonary epithelial cells from pig lung tissue.administration of drugs by inhalation opens new possibilities for entry into the systemic circulation and cultures of porcine pulmonary epithelial cells (pecs) may prove to be valuable in the prediction of pulmonary metabolism of drugs in humans. this paper, therefore, reports a method for the routine isolation and cultivation of pecs from slaughterhouse animals. on average 1.5x10(6) cells g-1 tissue were isolated by discontinuous density-gradient centrifugation. cells were subsequently cultivat ...200516393853
humoral and cell-mediated immune response in patients with malignant melanoma. i. in vitro lymphocyte reactivity to pha and antigens following immunization.in vitro lymphocyte function was evaluated in 61 patients with different clinical stages of malignant melanoma. thirty-one of these patients had localized disease, 13 regional metastases, 10 distant lymph node or skin metastases, and 7 visceral metastases. following immunization, in vitro lymphocyte reactivity to three antigens (diphtheria toxoid, tetanus toxoid and alpha-hemocyanin of helix pomatia) was studied in the presence of autologous serum, in addition to lymphocyte reactivity to phytohe ...20051175127
crassostrea gigas ferritin: cdna sequence analysis for two heavy chain type subunits and protein purification.ferritin has been shown as being the principal iron storage in the majority of living organisms. in marine species, ferritin is also involved in high-level accumulation of (210)po. as part of our work on the investigation of these radionuclides' concentration in natural environment, ferritin was searched at the gene and protein level. ferritin was purified from the visceral mass of the oyster crassostrea gigas by ion-exchange chromatography and hplc. sds-page revealed one band of 20 kda. an expr ...200415315822
structure and glycosylation of the term yolk sac placenta and uterine attachment site in the viviparous shark mustelus canis.the viviparous shark mustelus canis nurtures its young within the uterus by means of a modified yolk sac which functions as a placenta. two term specimens have been examined with a panel of 21 biotinylated lectins to assess whether glycans form a prominent interface between fetal and maternal tissues as in their therian counterparts. the yolk sac placenta was lined by a thin egg envelope which apposed though did not make contact with the uterine epithelium, and expressed fucosyl, n-acetyl glucos ...200415451197
monoclonal antibodies against the tn-specific isolectin b4 from vicia villosa seeds: characterization of the epitope of the blocking antibody vv34.vicia villosa isolectin b4 (vvlb4) recognizes the tn antigen (galnac-o-ser/thr) exposed in certain human carcinomas. we have produced anti-vvlb4 monoclonal antibodies (mabs), and their lectin recognition selectivity was assessed by elisa and western blot against the purified gal/galnac-specific lectins from vicia villosa, salvia sclarea, helix pomatia, arachis hypogaea, glycine max, and dolichos biflorus. the antibodies were also tested for their ability to block the binding of vvlb4 to the tn a ...200415000847
structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes.lung macro- and microvascular endothelial cells exhibit unique functional attributes, including signal transduction and barrier properties. we therefore sought to identify structural and functional features of endothelial cells that discriminate their phenotypes in the fully differentiated lung. rat lung macro- (paec) and microvascular (pmvec) endothelial cells each exhibited expression of typical markers. screening for reactivity with nine different lectins revealed that glycine max and griffon ...200415020205
use of taxol and collagenase for better three-dimensional visualization of microtubules in the enterocyte and brunner's gland cell, with special reference to their relation to the golgi apparatus.cytoskeletal microtubules were visualized in the mouse duodenal mucosa by an improved immunofluorescence method using a microtubule-stabilizing reagent, taxol, and collagenase as an enzymatic epitope retriever. the improvement in immunostaining was shown morphologically and statistically by comparing fluorescence intensities of specimens prepared with or without taxol and collagenase treatment. in free cells in the epithelium and in the lamina propria, microtubules radiated from the gamma-tubuli ...200415077902
amide n-glucuronidation of maxipost catalyzed by udp-glucuronosyltransferase 2b7 in humans.maxipost [(3s)-(+)-(5-chloro-2-methoxyphenyl)-1,3-dihydro-3-fluoro-6-(trifluoromethyl)-2h-indole-2-one), or bms-204352)] is a potent and specific maxi-k channel opener for potential use to treat stroke. this article describes structural characterization of a major human n-glucuronide metabolite of bms-204352 and identification of the enzyme responsible for the n-glucuronidation reaction. after intravenous administrations of [(14)c]bms-204352 (10 mg, 50 microci) to eight healthy human subjects, o ...200415100177
ability of blood group a-active glycosphingolipids to act as escherichia coli heat-labile enterotoxin receptors in ht-29 cells.we examined the ability of blood group a-active glycoconjugates to act as receptors for escherichia coli heat-labile type i enterotoxin (lt-i) in ht-29 cells. these cells contained ~4 times more specific binding sites for lt-i than for cholera toxin (ct). binding of lt-i could not be blocked by the b subunit of ct (ct-b), indicating the existence of lt-i receptors in addition to the glycosphingolipid gm1. lt-i was able to increase levels of cyclic adenosine monophosphate (amp), even in the prese ...200415116290
herpes simplex virus type-2 specific glycoprotein g-2 immunomagnetically captured from hep-2 infected tissue culture extracts.monoclonal antibody h1206 anti-hsv-2 gg-2 bound to tosylactivated paramagnetic dynabeads (dynal) has been used to isolate hsv-2 type-specific gg-2 from solubilized hep-2 hsv-2 infected cell extracts. the immunomagnetically captured type-specific glycoprotein reacted strongly with monoclonal antibody h1206 and demonstrated a single band with apparent molecular weight of 100000 (100 kda) and a doublet band with an apparent molecular weight of 60000-64000 (60-64 kda). we observed the same exact ban ...200415158593
in vitro capacitation and acrosome reaction of dog spermatozoa can be feasibly attained in a defined medium without glucose.incubation of dog spermatozoa in a medium without glucose and in the presence of lactate and pyruvate (l-ccm) for 4 h at 38.5 degrees c in a 5% co(2) atmosphere induced in vitro capacitation of these cells. this was verified after the combined specific capacitation-like changes in percentages of viability and altered acrosomes, motility characteristics, sperm location of reactivity against pisum sativum, arachis hypogaea and helix pomatia lectins and the tyrosine phosphorylation pattern. further ...200415182287
sperm binding to oviductal epithelial cells in the rat: role of sialic acid residues on the epithelial surface and sialic acid-binding sites on the sperm surface.the aim of this study was to assess the participation of carbohydrate residues in the adhesion of spermatozoa to the oviductal epithelium in the rat. we first examined, by lectin labeling, the distribution of glycoconjugates in rat oviducts obtained under various hormonal environments. several classes of glycoconjugates were abundant in the epithelium, and the expression of some of these molecules varied differentially in ampulla and isthmus, along the estrous cycle and with estradiol and proges ...200415201197
epileptogenicity and epileptic activity: mechanisms in an invertebrate model nervous system.epileptic seizures are based on paroxysmal depolarization shifts (pds) which are synchronized in many neurons. mechanisms underlying pds and seizures are still not understood. the present review is based on studies using the buccal ganglia of the snail helix pomatia as a model nervous system. essential mechanisms of epileptic activity in nervous systems are thought to be identical in whatever part of the human or animal nervous system epileptic activity appears. from studies using the buccal gan ...200415216913
functional morphology of the salivary gland of the snail, helix pomatia: a histochemical and immunocytochemical study.functional morphology of helix pomatia salivary gland cells was studied at light microscopic level by using different histochemical methods. three cell types could be demonstrated in the salivary gland: mucocytes, granular and vacuolated cells. the distribution and the number of the different cell types were different in active and inactive snails. in active feeding animals, dilatated interlobular salivary ducts were observed, which were never present in inactive ones. in active animals an addit ...200415270238
continuous increase of epileptogenic effects following application of proteolytic enzymes (buccal ganglia of helix pomatia).epileptic activity of neurons consists of paroxysmal depolarization shifts (pds) which can be induced presumably in any nervous system by application of an epileptogenic drug. the spontaneous appearance of epileptic activity, however, is based on a largely unknown process which increases susceptibility to epileptic activity (seizure susceptibility in man). it is presently shown that the treatment of ganglia with proteolytic enzymes (pronase) decreases the effective concentration of epileptogenic ...200415270243
supercooling ability in two populations of the land snail helix pomatia (gastropoda: helicidae) and ice-nucleating activity of gut bacteria.the land snail helix pomatia (gastropoda: helicidae) is widely distributed in northern and central europe where it may experience subzero temperatures during winter months. its supercooling ability was studied in two populations of h. pomatia. one population originated from southern sweden (gotaland) and the other from central france (auvergne). in the experimental design, they were acclimated, over 2 weeks, to artificial winter conditions (hibernation, t=5 degrees c). the swedish snails showed ...200415710369
the parasitic trematode fasciola hepatica exhibits mammalian-type glycolipids as well as gal(beta1-6)gal-terminating glycolipids that account for cestode serological cross-reactivity.neutral glycosphingolipids from sheep-derived fasciola hepatica liver flukes were isolated and characterized both structurally and serologically. after hplc fractionation, glycolipids were analyzed by linkage analysis, enzymatic cleavage, and maldi-tof as well as electrospray ionization mass spectrometry. obtained results revealed the presence of two types of neutral glycolipids. the first group represented mammalian-type species comprising globo- and isoglobotriaosylceramides (gal(alpha1-4)gal( ...200414638629
metabolism of the soy isoflavones daidzein, genistein and glycitein in human subjects. identification of new metabolites having an intact isoflavonoid skeleton.epidemiological studies have associated high soy intake with a lowered risk for certain hormone-dependent diseases. soy and soy foods are rich sources of isoflavones, which have been shown to possess several biological activities. in this study, the metabolism of soy isoflavones daidzein, genistein and glycitein was investigated in human subjects. the aim was to find and identify urinary phase i metabolites of isoflavones, which have an intact isoflavonoid skeleton, and which might possess some ...200314698210
leptomonas wallacei shows distinct morphology and surface carbohydrates composition along the intestinal tract of its host oncopeltus fasciatus (hemiptera: lygaeidae) and in axenic culture.leptomonas wallacei is a monoxenic trypanosomatid that colonizes the digestive tract of the phytophagous hemipteran oncopeltus fasciatus. this infection was specific and took place exclusively in midgut intestinal ventricles v3 and v4, and in the hindgut. abundances of parasites in the hindgut were 54% less than those in the hindgut. parasites in the hindgut were more slender and had a longer flagellum than those from the hindgut, which were rounded, with a shorter flagellum. moreover, hindgut f ...200314974433
exposed and hidden lectin-binding epitopes at the surface of borrelia burgdorferi.the presence of surface- and subsurface-located lectin-binding epitopes of borrelia burgdorferi was examined by electron microscopy using a variety of gold-labeled lectins. concanavalin a reacted predominantly with extracellular material adjacent to the spirochetes. wheat germ agglutinin bound weakly to the surface of borreliae; however, alterations of the outer membrane by preincubation in 100 ppm triton x-100 or boiling uncovered numerous periplasmic sites recognized by the lectin. the peripla ...200314976724
expression of cpgp40/15 in toxoplasma gondii: a surrogate system for the study of cryptosporidium glycoprotein antigens.cryptosporidium parvum is a waterborne enteric coccidian that causes diarrheal disease in a wide range of hosts. development of successful therapies is hampered by the inability to culture the parasite and the lack of a transfection system for genetic manipulation. the glycoprotein products of the cpgp40/15 gene, gp40 and gp15, are involved in c. parvum sporozoite attachment to and invasion of host cells and, as such, may be good targets for anticryptosporidial therapies. however, the function o ...200314500524
effect of sugars on the association between cowpea vicilin (7s storage proteins) and fungal cells.vicilins (7s storage proteins) found in various legume seeds have been previously shown to interfere with the germination of spores or conidia of phytopathogenic fungi and inhibit yeast growth and glucose stimulated acidification of the medium by yeast cells. in the present work vicilins from cowpea (vigna unguiculata) seeds were added to the growth medium of saccharomyces cerevisiae cells and fusarium oxysporum conidia. helix pomatia lectin, wheat germ agglutinin and ulex europaeus lectin were ...200314510235
optimized glucuronide hydrolysis for the detection of psilocin in human urine samples.in order to develop a sensitive and reliable analytical method for psilocin (pc) in urine samples, the hydrolysis conditions including the acid, alkaline and enzymatic hydrolyses have been investigated by monitoring not only pc but also psilocin glucuronide (pcg) by liquid chromatography tandem mass spectrometry (lc-ms-ms); pcg was initially identified in a "magic mushroom (mm)" user's urine by liquid chromatography mass spectrometry (lc-ms) and lc-ms-ms. the proposed conditions optimized for th ...200314581081
screening of clostebol and its metabolites in bovine urine with elisa and comparison with gc-ms results in an interlaboratory study.an extraction procedure for clostebol metabolites in urine is developed including enzymatic hydrolysis of conjugated metabolites with helix pomatia juice (shp) and solid-phase extraction (spe) with further cleanup of sample extracts. for the enzymatic deconjugation step, variables such as buffer ph, amount of enzyme, incubation time, and temperature are examined. for the spe step, different wash solutions and combinations with subsequent liquid-liquid extractions are examined. incurred bovine ur ...200312820743
the role of calcium in mucin packaging within goblet cells.recent reports hypothesize that calcium plays an important role in providing cationic shielding to keep negatively charged mucins condensed and tightly packed within mucus granules of goblet cells. vitamin d controls mineral ion homeostasis and intestinal calcium absorption, which is mediated by the nuclear vitamin d receptor (vdr). hypocalcemia is observed in mice in which the vdr has been ablated. the purpose of this study was to test the hypothesis that normal levels of calcium are required f ...200312823989
hemocyte-mediated phagocytosis and melanization in the mosquito armigeres subalbatus following immune challenge by bacteria.mosquitoes are important vectors of disease. these insects respond to invading organisms with strong cellular and humoral immune responses that share many similarities with vertebrate immune systems. the strength and specificity of these responses are directly correlated to a mosquito's ability to transmit disease. in the current study, we characterized the hemocytes (blood cells) of armigeres subalbatus by morphology (ultrastructure), lectin binding, enzyme activity, immunocytochemistry, and fu ...200312838409
validation of a simple avidin-biotin detection method for helix pomatia lectin (hpa) binding as a prognostic marker in cancer.helix pomatia lectin (hpa) binding is a marker of metastatic competence in several human cancers. the altered cellular glycosylation detected by hpa is of clinical interest and functional significance, but research has been hampered by uncertainty over appropriate and accessible histochemical methods. most studies have employed a complex multi-layered detection system localising binding of unconjugated hpa by layering with a polyclonal antibody to hpa, a biotinylated secondary antibody against t ...200313677613
bivalve immunity: comparisons between the marine mussel (mytilus edulis), the edible cockle (cerastoderma edule) and the razor-shell (ensis siliqua).much of the current knowledge concerning bivalve immunology and immunotoxicology has come from studies on the mussel genus, mytilus, or from the american oyster, crassostrea virginica. following a major oil spill, it was observed that the marine mussel, mytilus edulis, underwent significant immunosuppression but no oil-induced mortalities, while in contrast, mass mortalities were noted in the edible cockle, cerastoderma edule, and the razor-shell, ensis siliqua. a study comparing immune cells an ...200312892742
lectinocytochemical detection of apoptotic murine leukemia l1210 cells.various cytomorphologic and biochemical markers of apoptosis are found in different compartments (plasma membrane, cytoplasm, nucleus, and mitochondria) of target cells. although the plasma membrane is an easily accessible cellular compartment, relatively little is known about its changes during apoptosis. we investigated whether specific changes in the expression of plasma membrane glycoproteins take place during apoptosis and whether these changes could be used for a quantitative estimation of ...200314608636
cyanogenesis in glucosinolate-producing plants: carica papaya and carica quercifolia.(r)-2-(beta-d-glucopyranosyloxy)-2-phenylacetonitrile (prunasin) was isolated from carica papaya l. and c. quercifolia (a. st.-hil.) hieron. (syn. c. hastata brign.). earlier reported presence of cyclopentanoid cyanohydrin glycosides in c. papaya could not be confirmed, and no cyclopentanoid amino acids could be detected in extracts of c. papaya and c. quercifolia. conversion of [2,3,4,5,6-3h]phenylalanine into tritiated prunasin was demonstrated in both species. on the other hand, when the plan ...200212031445
carbohydrate expression profile of colorectal cancer cells is relevant to metastatic pattern and prognosis.carbohydrate expression of cancer cells is closely related to the metastatic nature of colorectal cancer. in the present study we investigated the relevance of carbohydrate expression profiles of colorectal cancer cells in the primary lesion to metastatic distribution patterns as well as prognosis in 134 cases. carbohydrate expression was estimated by histochemistry with 17 kinds of lectins and 3 kinds of lewis-related monoclonal antibodies (mabs), and correlations between the staining and clini ...200211918084
influence of helix pomatia enzyme preparations on the oxidative conversion of some clostebol acetate metabolites in urine.clostebol acetate (4-chloro-testosterone acetate) is an anabolic steroid used for fattening purposes in cattle breeding. to safeguard public health, its use has been prohibited by the european commission since 1986. screening for its urinary metabolites is therefore an important tool for the control of possible violations. because those metabolites appear conjugated to glucuronic acid or sulfate, deconjugation prior to analysis is necessary. this work describes the variability in results seen wi ...200211916018
[immobilization of immunoglobulins using lectins with an immune sensor method based on surface plasmon resonance].the effect of different lectins upon the response of immune sensor based on surface plasmon resonance (spr) was investigated. lectins affinity to carbohydrates of the igg can be used to increase the density and orientation of igg molecules at their immobilisation on the sensor surface. conditions were elaborated for enhancement of immune sensor response in comparison with that one for bare or preliminary treated with dodecanthiol thin gold sensor surface. it was shown that human igg revealed max ...200212152312
study of natural and artificial corticosteroid phase ii metabolites in bovine urine using hplc-ms/ms.corticosteroid compounds are widely used therapeutically for their anti-inflammatory properties and sometimes as growth promoters in food producing animals. in the field of drug residue analysis, knowledge of the main metabolic pathways of target analytes improves the efficiency of the corresponding control. thus, phase ii metabolism of corticosteroids, for which very little literature is available, was investigated in cattle. an lc-ms/ms detection method was developed for five commercially avai ...200212231123
some new aspects of 17alpha-estradiol metabolism in man.17alpha-estradiol (1,3,5(10)-estratriene-3,17alpha-diol) together with a tracer dose of the tritium-labeled compound was administered orally and sublingually to male volunteers. the serum concentrations of 17alpha-estradiol (free and liberated by enzymatic hydrolysis) were quantified by gc/ms, and the serum total radioactivity and urinary radioactivity excretion were determined. after oral administration, 17alpha-estradiol was rapidly and intensively conjugated; only tiny quantities of the free ...200212234624
investigations into the potential role of aberrant n-acetylgalactosamine glycans in tumour cell interactions with basement membrane components.the expression of the aberrant n-acetylgalactosamine (galnac) glycoconjugates, detected by binding of the lectin from helix pomatia (hpa) is reported to be associated with metastatic competence and poor prognosis in a range of human adenocarcinomas, but the functional significance of the glycoconjugates in metastatic mechanisms is unknown. we have employed seven cell lines derived from normal breast epithelium, primary breast cancer and breast cancer metastases which stably express varying level ...200212405285
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