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the nematicidal effect of camellia seed cake on root-knot nematode meloidogyne javanica of banana.suppression of root-knot nematodes is crucially important for maintaining the worldwide development of the banana industry. growing concerns about human and environmental safety have led to the withdrawal of commonly used nematicides and soil fumigants, thus motivating the development of alternative nematode management strategies. in this study, meloidogyne javanica was isolated, and the nematicidal effect of camellia seed cake on this pest was investigated. the results showed that in dish exper ...201525849382
the diverse nematicidal properties and biocontrol efficacy of bacillus thuringiensis cry6a against the root-knot nematode meloidogyne hapla.cry6a toxin from bacillus thuringiensis is a representative nematicidal crystal protein with a variety of nematicidal properties to free-living nematode caenorhabditis elegans. cry6a shares very low homology and different structure with cry5b, another representative nematicidal crystal protein, and probably acts in a distinct pathway. all these strongly indicate that cry6a toxin is likely a potent candidate for nematicide. the present study dealt with global investigation to determine the detrim ...201525556591
sequence composition of bac clones and ssr markers mapped to upland cotton chromosomes 11 and 21 targeting resistance to soil-borne pathogens.genetic and physical framework mapping in cotton (gossypium spp.) were used to discover putative gene sequences involved in resistance to common soil-borne pathogens. chromosome (chr) 11 and its homoeologous chr 21 of upland cotton (g. hirsutum) are foci for discovery of resistance (r) or pathogen-induced r (pr) genes underlying qtls involved in response to root-knot nematode (meloidogyne incognita), reniform nematode (rotylenchulus reniformis), fusarium wilt (fusarium oxysporum f.sp. vasinfectu ...201526483808
the application of arabidopsis thaliana in studying tripartite interactions among plants, beneficial fungal endophytes and biotrophic plant-parasitic nematodes.the research demonstrated that arabidopsis can be used as a model system for studying plant-nematode-endophyte tripartite interactions; thus, opening new possibilities for further characterizing the molecular mechanisms behind these interactions. arabidopsis has been established as an important model system for studying plant biology and plant-microbe interactions. we show that this plant can also be used for studying the tripartite interactions among plants, the root-knot nematode meloidogyne i ...201525548000
genetic mapping of resistance to meloidogyne arenaria in arachis stenosperma: a new source of nematode resistance for peanut.root-knot nematodes (rkn; meloidogyne sp.) are a major threat to crops in tropical and subtropical regions worldwide. the use of resistant crop varieties is the preferred method of control because nematicides are expensive, and hazardous to humans and the environment. peanut (arachis hypogaea) is infected by four species of rkn, the most damaging being m. arenaria, and commercial cultivars rely on a single source of resistance. in this study, we genetically characterize rkn resistance of the wil ...201526656152
pasteuria penetrans for control of meloidogyne incognita on tomato and cucumber, and m. arenaria on snapdragon.meloidogyne incognita and meloidogyne arenaria are important parasitic nematodes of vegetable and ornamental crops. microplot and greenhouse experiments were conducted to test commercial formulations of the biocontrol agent pasteuria penetrans for control of m. incognita on tomato and cucumber and m. arenaria on snapdragon. three methods of application for p. penetrans were assessed including seed, transplant, and post-plant treatments. efficacy in controlling galling and reproduction of the two ...201526527842
broad meloidogyne resistance in potato based on rna interference of effector gene 16d10.root-knot nematodes (meloidogyne spp.) are a significant problem in potato (solanum tuberosum) production. there is no potato cultivar with meloidogyne resistance, even though resistance genes have been identified in wild potato species and were introgressed into breeding lines. the objectives of this study were to generate stable transgenic potato lines in a cv. russet burbank background that carry an rna interference (rnai) transgene capable of silencing the 16d10 meloidogyne effector gene, an ...201525861119
mitochondrial genome plasticity among species of the nematode genus meloidogyne (nematoda: tylenchina).the mitochondrial (mt) genomes of the plant-parasitic root-knot nematodes meloidogyne arenaria, meloidogyne enterolobii and meloidogyne javanica were sequenced and compared with those of three other root-knot nematode species in order to explore the mt genome plasticity within meloidogyne. the mt genomes of m. arenaria, m. enterolobii and m. javanica are circular, with an estimated size of 18.8, 18.9 and 19.6 kb, respectively. compared to other nematodes these mt genomes are larger, due to the p ...201525655462
calcium is involved in the r mc1 (blb)-mediated hypersensitive response against meloidogyne chitwoodi in potato.functional characterization of the columbia root-knot nematode resistance gene r mc1 ( blb ) in potato revealed the r gene-mediated resistance is dependent on a hypersensitive response and involves calcium. the resistance (r) gene r mc1(blb) confers resistance against the plant-parasitic nematode, meloidogyne chitwoodi. avirulent and virulent nematodes were used to functionally characterize the r mc1(blb)-mediated resistance mechanism in potato (solanum tuberosum). histological observations indi ...201525315813
metagenomic insights into communities, functions of endophytes, and their associates with infection by root-knot nematode, meloidogyne incognita, in tomato roots.endophytes are known to play important roles in plant's health and productivity. in this study, we investigated the root microbiome of tomato in association with infection by root knot nematodes. our objectives were to observe the effects and response of the bacterial endophytes before nematode attacks and to reveal the functional attributes of microbes in plant health and nematode pathogenesis. community analysis of root-associated microbiomes in healthy and nematode-infected tomatoes indicated ...201526603211
pyramiding taro cystatin and fungal chitinase genes driven by a synthetic promoter enhances resistance in tomato to root-knot nematode meloidogyne incognita.meloidogyne incognita, one of the major root-knot nematode (rkn) species in agriculture, attacks many plant species, causing severe economic losses. genetic engineering of plants with defense-responsive genes has been demonstrated to control rkn. these studies, however, focused on controlling rkn at certain growth stages. in the present study, a dual gene overexpression system, utilizing a plant cysteine proteinase inhibitor (cecpi) and a fungal chitinase (pjchi-1), was used to transform tomato ...201525575993
in vitro nematicidal activity of aryl hydrazones and comparative gc-ms metabolomics analysis.a series of aryl hydrazones were synthesized and in vitro assayed for their activity on the root-knot nematode meloidogyne incognita. the phenylhydrazones of thiophene-2-carboxyaldehyde 5, 3-methyl-2-thiophenecarboxyaldehyde, 6, and salicylaldehyde, 2, were the most potent with ec50/48h values of 16.6 ± 2.2, 23.2 ± 2.7, and 24.3 ± 1.4 mg/l, respectively. a gc-ms metabolomics analysis, after in vitro nematode treatment with hydrazone 6 at 100 mg/l for 12 h, revealed elevated levels of fatty acids ...201526528945
nematicidal triterpenoids from lantana camara.a new triterpene, lancamarolide (1), and seven known triterpenes, oleanonic acid (2), lantadene a (3), 11α-hydroxy-3-oxours-12-en-28-oic acid (4), betulinic acid (5), lantadene b (6), and lantaninilic acid (7) were isolated from the aerial parts of lantana camara in the course of bioassay-guided isolation, and their nematicidal activities against meloidogyne incognita, the root knot nematode, were carried out. oleanonic acid was found to be the most active compound and exhibited 80% mortality af ...201526363887
management of root-knot nematode (meloidogyne incognita) in bottle gourd using different botanicals in pots.a pot experiment was conducted to study the efficacy of different botanicals in varying doses for management of root-knot nematode, m. incognita in bottle gourd. the results exhibited that madar (calotropis procera) and neem (azadirachta indica) leaves application proved to be more effective in improving plant growth characters and reducing root-knot index and final nematode population. among the doses tested, higher dose of 1.5 % (w/w) was more effective than lower ones.201526345048
mi-1-mediated nematode resistance in tomatoes is broken by short-term heat stress but recovers over time.tomato (solanum lycopersicum l.) is among the most valuable agricultural products, but meloidogyne spp. (root-knot nematode) infestations result in serious crop losses. in tomato, resistance to root-knot nematodes is controlled by the gene mi-1, but heat stress interferes with mi-1-associated resistance. inconsistent results in published field and greenhouse experiments led us to test the effect of short-term midday heat stress on tomato susceptibility to meloidogyne incognita race 1. under cont ...201526170475
yellow pigment aurovertins mediate interactions between the pathogenic fungus pochonia chlamydosporia and its nematode host.nematophagous fungi are globally distributed soil fungi and well-known natural predators of soil-dwelling nematodes. pochonia chlamydosporia can be found in diverse nematode-suppressive soils as a parasite of nematode eggs and is one of the most studied potential biological control agents of nematodes. however, little is known about the functions of small molecules in the process of infection of nematodes by this parasitic fungus or about small-molecule-mediated interactions between the pathogen ...201526151481
synthesis and nematicidal activities of 1,2,3-benzotriazin-4-one derivatives against meloidogyne incognita.a series of novel 1,2,3-benzotriazin-4-one derivatives were synthesized by the reaction of 3-bromoalkyl-1,2,3-benzotriazin-4-ones with potassium salt of 2-cyanoimino-4-oxothiazolidine in the presence of potassium iodide. nematicidal assays in vivo showed that some of them exhibited good control efficacy against the cucumber root-knot nematode disease caused by meloidogyne incognita, up to 100% at the concentration of 10.0 mg l(-1), which indicated that 1,2,3-benzotriazin-4-one derivatives might ...201526145729
overexpression of mic-3 indicates a direct role for the mic gene family in mediating upland cotton (gossypium hirsutum) resistance to root-knot nematode (meloidogyne incognita).transgene-based analysis of the mic-3 gene provides the first report of a cotton gene having a direct role in mediating cotton resistance to root-knot nematode. major quantitative trait loci have been mapped to upland cotton (gossypium hirsutum l.) chromosomes 11 and 14 that govern the highly resistant phenotype in response to infection by root-knot nematode (rkn; meloidogyne incognita); however, nearly nothing is known regarding the underlying molecular determinants of this rkn-resistant phenot ...201525376794
a meloidogyne incognita effector is imported into the nucleus and exhibits transcriptional activation activity in planta.root-knot nematodes are sedentary biotrophic endoparasites that maintain a complex interaction with their host plants. nematode effector proteins are synthesized in the oesophageal glands of nematodes and secreted into plant tissue through a needle-like stylet. effectors characterized to date have been shown to mediate processes essential for nematode pathogenesis. to gain an insight into their site of action and putative function, the subcellular localization of 13 previously isolated meloidogy ...201524863562
development and validation of lna-based quantitative real-time pcr assays for detection and identification of the root-knot nematode meloidogyne enterolobii in complex dna backgrounds.meloidogyne enterolobii is a quarantine root-knot nematode posing a major threat to agricultural production systems worldwide. it attacks many host plants, including important agricultural crops, ornamentals, and trees. m. enterolobii is a highly virulent and pathogenic root-knot nematode species, able to reproduce on plants resistant to other meloidogyne spp. significant crop damage has been reported in asia, south america, africa, the united states, france, and greenhouses in switzerland. to i ...201525775103
biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections.biochar is a solid coproduct of biomass pyrolysis, and soil amended with biochar has been shown to enhance the productivity of various crops and induce systemic plant resistance to fungal pathogens. the aim of this study was to explore the ability of wood biochar to induce resistance to the root-knot nematode (rkn) meloidogyne graminicola in rice (oryza sativa cv. nipponbare) and examine its histochemical and molecular impact on plant defense mechanisms.201526537003
intraspecific variability of the facultative meiotic parthenogenetic root-knot nematode (meloidogyne graminicola) from rice fields in vietnam.twenty years ago, the facultative meiotic parthenogenetic root-knot nematode (rkn), meloidogyne graminicola, was recognised as an important rice pathogen in south vietnam. although this country is one of the most important rice exporters worldwide, a comprehensive picture of the occurrence of m. graminicola in vietnamese rice fields is still not available. therefore a nematode survey was carried out with the aim of better understanding the geographical distribution, and the pathogenic and geneti ...201526026576
the role of thionins in rice defence against root pathogens.thionins are antimicrobial peptides that are involved in plant defence. here, we present an in-depth analysis of the role of rice thionin genes in defence responses against two root pathogens: the root-knot nematode meloidogyne graminicola and the oomycete pythium graminicola. the expression of rice thionin genes was observed to be differentially regulated by defence-related hormones, whereas all analysed genes were consistently down-regulated in m. graminicola-induced galls, at least until 7 da ...201525676661
β-aminobutyric acid-induced resistance against root-knot nematodes in rice is based on increased basal defense.the nonprotein amino acid β-aminobutyric acid (baba) is known to protect plants against various pathogens. the mode of action is relatively diverse and specific in different plant-pathogen systems. to extend the analysis of the mode of action of baba to plant-parasitic nematodes in monocot plants, we evaluated the effect of baba against the root-knot nematode (rkn) meloidogyne graminicola in rice. baba treatment of rice plants inhibited nematode penetration and resulted in delayed nematode and g ...201525608179
impact of grapevine (vitis vinifera) varieties on reproduction of the northern root-knot nematode (meloidogyne hapla).one of the most commonly encountered plant-parasitic nematodes in eastern washington vitis vinifera vineyards is meloidogyne hapla; however, limited research exists on the impact of this nematode on v. vinifera. the objectives of this research were to determine the impact of m. hapla on chardonnay and cabernet sauvignon vine establishment and to determine the host status of v. vinifera varieties/clones predominantly grown in washington to m. hapla. in a microplot experiment, chardonnay and caber ...201526170476
first report of northern root-knot nematode, meloidogyne hapla, parasitic on oaks, quercus brantii and q. infectoria in iran.root-knot nematodes (rkn) are the most serious plant parasitic nematodes having a broad host range exceeding 2,000 plant species. quercus brantii lindl. and q. infectoria oliv are the most important woody species of zagros forests in west of iran where favors sub-mediterranean climate. national botanical garden of iran (nbgi) is scheduled to be the basic center for research and education of botany in iran. this garden, located in west of tehran, was established in 1968 with an area of about 150 ...201525861121
nematicidal activity of the volatilome of eruca sativa on meloidogyne incognita.research on new pesticides based on plant extracts, aimed at the development of nontoxic formulates, has recently gained increased interest. this study investigated the use of the volatilome of rucola (eruca sativa) as a powerful natural nematicidal agent against the root-knot nematode, meloidogyne incognita. analysis of the composition of the volatilome, using gc-ms-spme, showed that the compound (z)-3-hexenyl acetate was the most abundant, followed by (z)-3-hexen-1-ol and erucin, with relative ...201526082278
proteome of soybean seed exudates contains plant defense-related proteins active against the root-knot nematode meloidogyne incognita.several studies have described the effects of seed exudates against microorganisms, but only few of them have investigated the proteins that have defensive activity particularly against nematode parasites. this study focused on the proteins released in the exudates of soybean seeds and evaluated their nematicidal properties against meloidogyne incognita. a proteomic approach indicated the existence of 63 exuded proteins, including β-1,3-glucanase, chitinase, lectin, trypsin inhibitor, and lipoxy ...201526034922
knock-down of heat-shock protein 90 and isocitrate lyase gene expression reduced root-knot nematode reproduction.crop losses caused by nematode infections are estimated to be valued at usd 157 billion per year. meloidogyne incognita, a root-knot nematode (rkn), is considered to be one of the most important plant pathogens due to its worldwide distribution and the austere damage it can cause to a large variety of agronomically important crops. rna interference (rnai), a gene silencing process, has proven to be a valuable biotechnology alternative method for rkn control. in this study, the rnai approach was ...201526020830
tomato transgenic plants expressing hairpin construct of a nematode protease gene conferred enhanced resistance to root-knot nematodes.root-knot nematodes (meloidogyne incognita) cause substantial yield losses in vegetables worldwide, and are difficult to manage. continuous withdrawal of environmentally-harmful nematicides from the global market warrants the need for novel nematode management strategies. utility of host-delivered rnai has been demonstrated in several plants (arabidopsis, tobacco, and soybean) that exhibited resistance against root-knot and cyst nematodes. herein, a m. incognita-specific protease gene, cathepsin ...201525883594
transcriptome analysis of resistant and susceptible alfalfa cultivars infected with root-knot nematode meloidogyne incognita.nematodes are one of the major limiting factors in alfalfa production. root-knot nematodes (rkn, meloidogyne spp.) are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. as of today, no studies have been published on global gene expression profiling in alfalfa infected with rkn or any other plant parasitic nematode. very little information is available about molecular mechanisms that contribute to ...201525822722
associated bacteria of different life stages of meloidogyne incognita using pyrosequencing-based analysis.the root knot nematode (rkn), meloidogyne incognita, belongs to the most damaging plant pathogens worldwide, and is able to infect almost all cultivated plants, like tomato. recent research supports the hypothesis that bacteria often associated with plant-parasitic nematodes, function as nematode parasites, symbionts, or commensal organisms etc. in this study, we explored the bacterial consortia associated with m. incognita at different developmental stages, including egg mass, adult female and ...201525809195
cowpea-meloidogyne incognita interaction: root proteomic analysis during early stages of nematode infection.cowpea (vigna unguiculata l. walp) is an important legume species well adapted to low fertility soils and prolonged drought periods. one of the main problems that cause severe yield losses in cowpea is the root-knot nematode meloidogyne incognita. the aim of this work was to analyze the differential expression of proteins in the contrasting cultivars of cowpea ce 31 (highly resistant) and ce 109 (slightly resistant) during early stages of m. incognita infection. cowpea roots were collected at 3, ...201525736976
transcriptome analysis of resistant and susceptible alfalfa cultivars infected with root-knot nematode meloidogyne incognita.nematodes are one of the major limiting factors in alfalfa production. root-knot nematodes (rkn, meloidogyne spp.) are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. as of today, no studies have been published on global gene expression profiling in alfalfa infected with rkn or any other plant parasitic nematode. very little information is available about molecular mechanisms that contribute to ...201525710378
maturation of nematode-induced galls in medicago truncatula is related to water status and primary metabolism modifications.root-knot nematodes are obligatory plant parasitic worms that establish and maintain an intimate relationship with their host plants. during a compatible interaction, these nematodes induce the redifferentiation of root cells into multinucleate and hypertrophied giant cells (gcs). these metabolically active feeding cells constitute the exclusive source of nutrients for the nematode. we analyzed the modifications of water status, ionic content and accumulation of metabolites in development and ma ...201525617326
resistance of upland-rice lines to root-knot nematode, meloidogyne incognita.despite the benefits of crop rotation, occurrence of nematodes is a common problem for almost all crops within the cerrado biome, especially for rice. the use of resistant cultivars is one of the main methods for control of nematodes. thus, the present study aimed to evaluate the reaction of 36 upland-rice lines, with desirable agronomic characteristics, according to their resistance to root-knot nematodes (meloidogyne incognita). the experimental design was entirely randomized with four replica ...201526782379
molecular identification and functional characterization of the fatty acid- and retinoid-binding protein gene rs-far-1 in the burrowing nematode radopholus similis (tylenchida: pratylenchidae).fatty acid- and retinoid-binding protein (far) is a nematode-specific protein expressed in the nematode hypodermis. it is involved in nematode development, reproduction, and infection and can disrupt the plant defense reaction. in this study, we obtained the full-length sequence of the far gene from radopholus similis (rs-far-1), which is 828 bp long and includes a 558 bp orf encoding 186 amino acids. a protein homology analysis revealed that rs-far-1 is 75% similar to mj-far-1 from meloidogyne ...201525734501
draft genome sequence of stenotrophomonas maltophilia strain b418, a promising agent for biocontrol of plant pathogens and root-knot nematode.stenotrophomonas maltophilia strain b418 was isolated from a barley rhizosphere in china. this bacterium exhibits broad-spectrum inhibitory activities against plant pathogens and root-knot nematode along with growth-promoting effects. here, we present the draft genome sequence of s. maltophilia b418.201525700397
phenotyping nematode feeding sites: three-dimensional reconstruction and volumetric measurements of giant cells induced by root-knot nematodes in arabidopsis.the control of plant parasitic nematodes is an increasing problem. a key process during the infection is the induction of specialized nourishing cells, called giant cells (gcs), in roots. understanding the function of genes required for gc development is crucial to identify targets for new control strategies. we propose a standardized method for gc phenotyping in different plant genotypes, like those with modified genes essential for gc development. the method combines images obtained by bright- ...201525613856
instrumental and sensory analyses of quality attributes of grafted specialty melons.soilborne disease management remains a great challenge in melon production with the phaseout of soil fumigant methyl bromide. grafting has been shown to be an effective approach to control soilborne diseases. however, previous research has yielded mixed results regarding the impacts of rootstock on fruit quality. very few studies have assessed melon quality attributes using both sensory evaluation and instrumental methods.201525512001
disruption of prefoldin-2 protein synthesis in root-knot nematodes via host-mediated gene silencing efficiently reduces nematode numbers and thus protects plants.the aim of this study is to demonstrate the feasibility of down-regulating endogeneous prefoldin-2 root-knot nematode transcripts by expressing dsrna with sequence identity to the nematode gene in tobacco roots under the influence of strong arabidopsis ubiquitin (ubq1) promoter. root-knot nematodes (rkns) are sedentary endoparasites infecting a wide range of plant species. they parasitise the root system, thereby disrupting water and nutrient uptake and causing major reductions in crop yields. t ...201525491640
sclareol induces plant resistance to root-knot nematode partially through ethylene-dependent enhancement of lignin accumulation.the root-knot nematode (rkn) is one of the most devastating parasitic nematodes of plants. although some secondary metabolites released by the host plant play roles as defense substances against parasitic nematodes, the mechanism underlying the induction of such defense responses is not fully understood. we found that sclareol, a natural diterpene known as an antimicrobial and defense-related molecule, inhibited rkn penetration of tomato and arabidopsis roots. sclareol induced genes related to e ...201525423264
nematicidal activity of furanocoumarins from parsley against meloidogyne spp.this report describes activity against meloidogyne spp. and chemical characterisation of the essential oil and methanol extract of petroselinum crispum aerial parts. the study was based on the hypothesis that p. crispum could be used as an intercrop and soil amendment in tomato culture for nematode control.201525157855
conversion of food waste into biofertilizer for the biocontrol of root knot nematode by paecilomyces lilacinus.the feasibility of converting food waste into nematocidal biofertilizer by nematophagous fungus paecilomyces lilacinus (p. lilacinus) was investigated. the culture conditions of p. lilacinus were optimized through response surface methodology. results showed that fermentation time, the amount of food waste, initial ph and temperature were most important factors for p. lilacinus production. the p. lilacinus production under optimized conditions was 10(9.6 ± 0.3) conidia ml⁻¹. after fermentation, ...201526075798
isolation and characterization of multifunctional streptomyces species with antimicrobial, nematicidal and phytohormone activities from marine environments in egypt.different strategies have been employed for selective isolation of streptomycetes from 20 marine samples varied in their biological nature. the recovery of streptomycetes isolates (112) was influenced preferentially by different strategies; sediment samples were the best source of potential candidate streptomycetes. all isolates exhibited antimicrobial activities with variable spectrum; the most promising isolates (31) were phenotypically characterized and identified as streptomyces sp.; these i ...201525805507
molecular characterisation and diagnosis of root-knot nematodes (meloidogyne spp.) from turfgrasses in north carolina, usa.root-knot nematodes (meloidogyne spp.) are the most common and destructive plant-parasitic nematode group worldwide and adversely influence both crop quality and yield. in this study, a total of 51 root-knot nematode populations from turfgrasses were tested, of which 44 were from north carolina, 6 from south carolina and 1 from virginia. molecular characterisation was performed on these samples by dna sequencing on the ribosomal dna 18s, its and 28s d2/d3. species-specific primers were developed ...201526599462
the transcriptome of nacobbus aberrans reveals insights into the evolution of sedentary endoparasitism in plant-parasitic nematodes.within the phylum nematoda, plant-parasitism is hypothesized to have arisen independently on at least four occasions. the most economically damaging plant-parasitic nematode species, and consequently the most widely studied, are those that feed as they migrate destructively through host roots causing necrotic lesions (migratory endoparasites) and those that modify host root tissue to create a nutrient sink from which they feed (sedentary endoparasites). the false root-knot nematode nacobbus aber ...201425123114
parasitic success without sex – the nematode experience.asexual reproduction is usually considered as an evolutionary dead end, and difficulties for asexual lineages to adapt to a fluctuating environment are anticipated due to the lack of sufficient genetic plasticity. yet, unlike their sexual congeners, mitotic parthenogenetic root-knot nematode species, meloidogyne spp., are remarkably widespread and polyphagous, with the ability to parasitize most flowering plants. although this may reflect in part the short-term stability of agricultural environm ...201425105196
members of the meloidogyne avirulence protein family contain multiple plant ligand-like motifs.sedentary plant-parasitic nematodes engage in complex interactions with their host plants by secreting effector proteins. some effectors of both root-knot nematodes (meloidogyne spp.) and cyst nematodes (heterodera and globodera spp.) mimic plant ligand proteins. most prominently, cyst nematodes secrete effectors that mimic plant clavata3/esr-related (cle) ligand proteins. however, only cyst nematodes have been shown to secrete such effectors and to utilize cle ligand mimicry in their interactio ...201425014776
a simple and efficient synthesis of fused morpholine pyrrolidines/piperdines with potential insecticidal activities.a simple and efficient synthesis of fused morpholine pyrrolidine/piperdine core structures was accomplished via a petasis-borono mannich reaction of cyclic amino alcohols, glyoxal and arylboronic acids with moderate to good yields. the bioassay data showed that the synthesized compounds displayed selective insecticidal activities against armyworm and root-knot nematode.201424927942
plant-parasitic nematode infections in rice: molecular and cellular insights.being one of the major staple foods in the world, and an interesting model monocot plant, rice (oryza sativa l.) has recently received attention from molecular nematologists studying the cellular and molecular aspects of the interaction between this crop and plant-parasitic nematodes. in this review, we highlight recent advances in this field, with a focus on the best-studied root-knot nematodes. histological studies have revealed the cellular changes inside root-knot nematode-induced feeding si ...201424906129
a role for lateral organ boundaries-domain 16 during the interaction arabidopsis-meloidogyne spp. provides a molecular link between lateral root and root-knot nematode feeding site development.plant endoparasitic nematodes induce the formation of their feeding cells by injecting effectors from the esophageal glands into root cells. although vascular cylinder cells seem to be involved in the formation of root-knot nematode (rkn) feeding structures, molecular evidence is scarce. we address the role during gall development of lateral organ boundaries-domain 16 (lbd16), a key component of the auxin pathway leading to the divisions in the xylem pole pericycle (xpp) for lateral root (lr) fo ...201424803293
re-evaluation of the inheritance for root-knot nematode resistance in the upland cotton germplasm line m-120 rnr revealed two epistatic qtls conferring resistance.we report a second major qtl for root-knot nematode resistance in the highly resistant upland cotton line m-120rnr and show epistasis between two resistant qtls with different mechanisms conferring resistance. in an earlier study, we identified a major qtl on chromosome 11 associated with resistance to root-knot nematode in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source. herein, we re-evaluated the genetics of the resistance to root-knot nematode in the m-1 ...201424728014
responses of tomato genotypes to avirulent and mi-virulent meloidogyne javanica isolates occurring in israel.the behavior of naturally virulent meloidogyne isolates toward the tomato resistance gene mi in major tomato-growing areas in israel was studied for the first time. virulence of seven selected isolates was confirmed over three successive generations on resistant (mi-carrying) and susceptible (non-mi-carrying) tomato cultivars. diagnostic markers verified the predominance of meloidogyne javanica among virulent isolates selected on resistant tomato cultivars or rootstocks. to better understand the ...201424724816
robust in vitro assay system for quantitative analysis of parasitic root-knot nematode infestation using lotus japonicus.root-knot nematodes are sedentary endoparasites that induce permanent infestation sites inside the roots of a broad range of crop plants. the development of effective control strategies require understanding the root-knot nematode parasitic process, however, the key molecular determinants for host manipulation during infestation remain elusive. one limiting factor has been the lack of a standardized conventional method for quantitative measurement of host parasitism by root-knot nematodes, parti ...201424704340
nematicidal activity of microbial pigment from serratia marcescens.ineffectiveness of available nematicides and the high damage caused by plant-parasitic nematodes result in the urgent need to find some natural remedy for their control. bioactivity of the pigment extracted from serratia marcescens was screened for controlling nematodes at their juvenile stage. test pigment was found effective against juvenile stages of radopholus similis and meloidogyne javanica at low concentrations (lc50 values, 83 and 79 μg/ml, respectively) as compared with positive control ...201424697144
nematicidal effects of silver nanoparticles on root-knot nematode in bermudagrass.certain nematodes are common soilborne organisms found in turfgrass in the united states that cause significant economic damage to golf course turf. one of the most prevalent plant-parasitic nematodes infesting turfgrass are root-knot nematodes (meloidogyne spp.). chemical treatment options for root-knot nematodes in turfgrass are limited, and there is a need for new nematicidal active ingredients to address this problem. in this study, we evaluated the use of silver nanoparticles (agnp) as a po ...201425275999
coexistence of minicircular and a highly rearranged mtdna molecule suggests that recombination shapes mitochondrial genome organization.recombination has been proposed as a possible mechanism to explain mitochondrial (mt) gene rearrangements, although the issue of whether mtdna recombination occurs in animals has been controversial. in this study, we sequenced the entire mt genome of the megaspilid wasp conostigmus sp., which possessed a highly rearranged mt genome. the sequence of the a+t-rich region contained a number of different types of repeats, similar to those reported previously in the nematode meloidogyne javanica, in w ...201424336845
vascularization and nutrient delivery at root-knot nematode feeding sites in host roots.plants are constantly challenged by pathogens and pests, which can have a profound impact on the yield and quality of produce in agricultural systems. the vascular system of higher plants is critical for growth and for their ability to counteract changing external conditions, serving as a distribution network for water, nutrients, and photosynthates from the source organs to regions where they are in demand. unfortunately, these features also make it an attractive target for pathogens and pests ...201424336493
plant resistance to aphid feeding: behavioral, physiological, genetic and molecular cues regulate aphid host selection and feeding.aphids damage major world food and fiber crops through direct feeding and transmission of plant viruses. fortunately, the development of many aphid-resistant crop plants has provided both ecological and economic benefits to food production. plant characters governing aphid host selection often dictate eventual plant resistance or susceptibility to aphid herbivory, and these phenotypic characters have been successfully used to map aphid resistance genes. aphid resistance is often inherited as a d ...201424282145
expression of phenylalanine ammonia lyase genes in maize lines differing in susceptibility to meloidogyne incognita.maize is a well-known host for meloidogyne incognita, and there is substantial variation in host status among maize genotypes. in previous work it was observed that nematode reproduction increased in the moderately susceptible maize inbred line b73 when the zmlox3 gene from oxylipid metabolism was knocked out. additionally, in this mutant line, use of a nonspecific primer for phenyl alanine ammonialyase (pal) genes indicated that expression of these genes was reduced in the mutant maize plants w ...201425580029
response of resistant and susceptible bell pepper (capsicum annuum) to a southern california meloidogyne incognita population from a commercial bell pepper field.to determine the presence and level of root-knot nematode (meloidogyne spp.) infestation in southern california bell pepper (capsicum annuum) fields, soil and root samples were collected in april and may 2012 and analyzed for the presence of root-knot nematodes. the earlier samples were virtually free of root-knot nematodes, but the later samples all contained, sometimes very high numbers, of root-knot nematodes. nematodes were all identified as m. incognita. a nematode population from one of th ...201425580027
evaluation of 31 potential biofumigant brassicaceous plants as hosts for three meloiodogyne species.brassicaceous cover crops can be used for biofumigation after soil incorporation of the mowed crop. this strategy can be used to manage root-knot nematodes (meloidogyne spp.), but the fact that many of these crops are host to root-knot nematodes can result in an undesired nematode population increase during the cultivation of the cover crop. to avoid this, cover crop cultivars that are poor or nonhosts should be selected. in this study, the host status of 31 plants in the family brassicaceae for ...201425276003
fast assembly of the mitochondrial genome of a plant parasitic nematode (meloidogyne graminicola) using next generation sequencing.little is known about the variations of nematode mitogenomes (mtdna). sequencing a complete mtdna using a pcr approach remains a challenge due to frequent genome reorganizations and low sequence similarities between divergent nematode lineages. here, a genome skimming approach based on hiseq sequencing (shotgun) was used to assemble de novo the first complete mtdna sequence of a root-knot nematode (meloidogyne graminicola). an at-rich genome (84.3%) of 20,030 bp was obtained with a mean sequenci ...201424841955
inheritance and mapping of mj-2, a new source of root-knot nematode (meloidogyne javanica) resistance in carrot.root-knot nematodes limit carrot production around the world by inducing taproot forking and galling deformities that render carrots unmarketable. in warmer climates, meloidogyne javanica and meloidogyne incognita are most prevalent. in f2 and f3 progeny from the cross between an asian carrot resistant to m. javanica, pi 652188, and a susceptible carrot, resistance response was incompletely dominant with a relatively high heritability (h (2) = 0.78) and provided evidence for a single gene, desig ...201424336925
effects of meloidogyne incognita and thielaviopsis basicola on cotton growth and root morphology.effects of the root-knot nematode meloidogyne incognita and the fungal pathogen thielaviopsis basicola on cotton seedling growth and root morphology were evaluated in controlled environmental experiments. four pathogen treatments, including noninfested soil, soil infested with m. incognita, soil infested with t. basicola, and soil infested with both pathogens were evaluated at soil bulk densities (bds) of 1.25 and 1.50 g/cm(3). plant growth and the morphology of the root systems were evaluated 4 ...201424283540
screening and histopathological characterization of korean carrot lines for resistance to the root-knot nematode meloidogyne incognita.in total, 170 carrot lines developed in korea were screened for resistance to meloidogyne incognita race 1 to select parental genetic resources useful for the development of nematode-resistant carrot cultivars. using the gall index (gi), gall formation was examined on carrot roots inoculated with approximately 1,000 second-stage juveniles of the nematode 7 weeks after inoculation. sixty-one carrot lines were resistant (gi ≤ 1.0), while the other 109 were susceptible (gi > 1.0) with coefficient o ...201425288988
the feeding behavior of adult root-knot nematodes (meloidogyne incognita) in rose balsam and tomato.meloidogyne incognita is a parasitic root-knot nematode that causes considerable yield loss in a wide range of plants. in this study we documented the movement of adult female nematodes for more than 2 hr in micro-slices of infected tomato (solanum lycopersicum) and rose balsam (impatiens balsamina) plants using light and video microscopy. stylet thrusting was followed by short pumping actions of the esophagus, dorsal esophageal gland ampulla, and metacorpal bulb. regular thrusting was normally ...201425276004
identification of msp1 gene variants in populations of meloidogyne incognita using pcr-dgge.effectors of root-knot nematodes are essential for parasitism and prone to recognition by adapted variants of the host plants. this selective pressure initiates hypervariability of effector genes. diversity of the gene variants within nematode populations might correlate with host preferences. in this study we developed a method to compare the distribution of variants of the effector gene msp1 among populations of meloidogyne incognita. primers were designed to amplify a 234-bp fragment of msp1. ...201425276001
benzothiadiazole effect in the compatible tomato-meloidogyne incognita interaction: changes in giant cell development and priming of two root anionic peroxidases.bth application is effective in root-knot nematode-tomato interaction in a way that involves a delay in the formation of nematode feeding site and triggers molecular responses at several levels. the compatible interaction between root-knot nematodes and their hosts requires the nematode to overcome plant defense systems so that a sophisticated permanent feeding site (giant cells) can be produced within the host roots. it has been suggested that activators of plant defenses may provide a novel ma ...201425085693
ovicidal activity of lactic acid produced by lysobacter capsici ys1215 on eggs of root-knot nematode, meloidogyne incognita.lysobacter capsici ys1215 isolated from soil previously showed nematicidal potential for biological control of the root-knot nematode. in this study, lactic acid, a nematicidal compound, was isolated from culture filtrate of ys1215, and its ovicidal activity was investigated. purification and identification of lactic acid were performed by a series of column chromatographies and identified by (1)h and (13)c nmr spectra and gc-ms analysis. our results showed that bacterial culture filtrate contai ...201425085571
effect of supreme 15 cs against meloidogyne incognita on tomato.pot and laboratory experiments were conducted to study the efficacy of supreme 15 cs in varying doses for management of root- knot nematode, m. incognita in tomato. the results indicated that supreme 15 cs containing alkali metal n-(n-propyl) dithiocarbamate proved to be more effective in improving plant growth characters and reducing root-knot index and final nematode population. among the doses tested, higher dose of 0.5 % (w/w) was more effective than lower doses.201425035599
development of enzyme linked immunosorbent assay (elisa) for the detection of root-knot nematode meloidogyne incognita.root-knot nematodes (meloidogyne incognita) are obligate, sedentary plant endoparasites that are extremely polyphagous in nature and cause severe economic losses in agriculture. hence, it is essential to control the parasite at an early stage. for any control strategy to be effective, an early and accurate diagnosis is of paramount importance. immunoassays have the inherent advantages of sensitivity and specificity; have the potential to identify and quantify these plant-parasitic nematodes. hen ...201425035590
management of meloidogyne incognita with chemicals and cultivars in cotton in a semi-arid environment.management of meloidogyne incognita (root-knot nematode) in cotton in the united states was substantially affected by the decision to stop production of aldicarb by its principle manufacturer in 2011. the remaining commercially available tools to manage m. incognita included soil fumigation, nematicide seed treatments, postemergence nematicide application, and cultivars partially resistant to m. incognita. small plot field studies were conducted on a total of nine sites from 2011-2013 to examine ...201424987161
management of meloidogyne incognita on tomato with endophytic bacteria and fresh residue of wasabia japonica.to characterize the nematicidal endophytic bacteria (neb) of wasabia japonica (wasabi) and evaluated the control efficacies of promising neb as well as fresh wasabi residue (fwr) against meloidogyne incognita on tomato.201424965702
elucidating the molecular bases of epigenetic inheritance in non-model invertebrates: the case of the root-knot nematode meloidogyne incognita.root-knot nematodes of the genus meloidogyne are biotrophic plant parasites that exhibit different life cycles and reproduction modes, ranging from classical amphimixis to obligatory mitotic parthenogenesis (apomixis), depending on the species. meloidogyne incognita, an apomictic species, exhibits a worldwide distribution and a wide host range affecting more than 3000 plant species. furthermore, evidences suggest that apomixis does not prevent m. incognita from adapting to its environment in con ...201424936189
control of meloidogyne incognita using mixtures of organic acids.this study sought to control the root-knot nematode (rkn) meloidogyne incognita using benign organo-chemicals. second-stage juveniles (j2) of rkn were exposed to dilutions (1.0%, 0.5%, 0.2%, and 0.1%) of acetic acid (aa), lactic acid (la), and their mixtures (mx). the nematode bodies were disrupted severely and moderately by vacuolations in 0.5% of mx and single organic acids, respectively, suggesting toxicity of mx may be higher than aa and la. the mortality of j2 was 100% at all concentrations ...201425506312
enhanced synthesis of antioxidant enzymes, defense proteins and leghemoglobin in rhizobium-free cowpea roots after challenging with meloydogine incognita.the root knot nematodes (rkn), meloydogine spp., particularly meloidogyne incognita and meloidogyne javanica species, parasitize several plant species and are responsible for large annual yield losses all over the world. only a few available chemical nematicides are still authorized for rkn control owing to environmental and health reasons. thus, plant resistance is currently considered the method of choice for controlling rkn, and research performed on the molecular interactions between plants ...201428250394
lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts mi-flp-18 expression to block infection.tomato (solanum lycopersicum) crops can be severely damaged due to parasitism by the root-knot nematode (rkn) meloidogyne incognita, but are protected when intercropped with crown daisy (chrysanthemum coronarium l.). root exudate may be the determining factor for this protection. an experiment using pots linked by a tube and petri dish experiments were undertaken to confirm that tomato-crown daisy intercropping root exudate decreased the number of nematodes and alleviated nematode damage, and to ...201424170741
mitochondrial genomes of meloidogyne chitwoodi and m. incognita (nematoda: tylenchina): comparative analysis, gene order and phylogenetic relationships with other nematodes.root-knot nematodes (meloidogyne spp.) are among the most important plant pathogens. in this study, the mitochondrial (mt) genomes of the root-knot nematodes, m. chitwoodi and m. incognita were sequenced. pcr analyses suggest that both mt genomes are circular, with an estimated size of 19.7 and 18.6-19.1kb, respectively. the mt genomes each contain a large non-coding region with tandem repeats and the control region. the mt gene arrangement of m. chitwoodi and m. incognita is unlike that of othe ...201424751670
a distinct role of pectate lyases in the formation of feeding structures induced by cyst and root-knot nematodes.pectin in the primary plant cell wall is thought to be responsible for its porosity, charge density, and microfibril spacing and is the main component of the middle lamella. plant-parasitic nematodes secrete cell wall-degrading enzymes that macerate the plant tissue, facilitating the penetration and migration within the roots. in sedentary endoparasitic nematodes, these enzymes are released only during the migration of infective juveniles through the root. later, nematodes manipulate the express ...201424905398
two closely related members of arabidopsis 13-lipoxygenases (13-loxs), lox3 and lox4, reveal distinct functions in response to plant-parasitic nematode infection.the responses of two closely related members of arabidopsis 13-lipoxygenases (13-loxs), lox3 and lox4, to infection by the sedentary nematodes root-knot nematode (meloidogyne javanica) and cyst nematode (heterodera schachtii) were analysed in transgenic arabidopsis seedlings. the tissue localization of lox3 and lox4 gene expression using β-glucuronidase (gus) reporter gene constructs showed local induction of lox3 expression when second-stage juveniles reached the vascular bundle and during the ...201424286169
phenotypic comparison of clinical and plant-beneficial strains of pantoea agglomerans.certain strains of pantoea are used as biocontrol agents for the suppression of plant diseases. however, their commercial registration is hampered in some countries because of biosafety concerns. this study compares clinical and plant-beneficial strains of p. agglomerans and related species using a phenotypic analysis approach in which plant-beneficial effects, adverse effects in nematode models, and toxicity were evaluated. plant-beneficial effects were determined as the inhibition of apple fru ...201426418852
manipulation of two α-endo-β-1,4-glucanase genes, atcel6 and gmcel7, reduces susceptibility to heterodera glycines in soybean roots.plant endo-β-1,4-glucanases (egases) include cell wall-modifying enzymes that are involved in nematode-induced growth of syncytia (feeding structures) in nematode-infected roots. egases in the α- and β-subfamilies contain signal peptides and are secreted, whereas those in the γ-subfamily have a membrane-anchoring domain and are not secreted. the arabidopsis α-egase at1g48930, designated as atcel6, is known to be down-regulated by beet cyst nematode (heterodera schachtii) in arabidopsis roots, wh ...201424844661
field efficacy of furfural as a nematicide on turf.a commercial formulation of furfural was recently launched in the united states as a turfgrass nematicide. three field trials evaluated efficacy of this commercial formulation on dwarf bermudagrass putting greens infested primarily with belonolaimus longicaudatus, meloidogyne graminis, or both these nematodes, and in some cases with mesocriconema ornatum or helicotylenchus pseudorobustus. in all these trials, furfural improved turf health but did not reduce population densities of b. longicaudat ...201424644368
bacterial antagonists of fungal pathogens also control root-knot nematodes by induced systemic resistance of tomato plants.the potential of bacterial antagonists of fungal pathogens to control the root-knot nematode meloidogyne incognita was investigated under greenhouse conditions. treatment of tomato seeds with several strains significantly reduced the numbers of galls and egg masses compared with the untreated control. best performed bacillus subtilis isolates sb4-23, mc5-re2, and mc2-re2, which were further studied for their mode of action with regard to direct effects by bacterial metabolites or repellents, and ...201424587352
exploitation of fta cartridges for the sampling, long-term storage, and dna-based analyses of plant-parasitic nematodes.the use of dna-based analyses in molecular plant nematology research has dramatically increased over recent decades. therefore, the development and adaptation of simple, robust, and cost-effective dna purification procedures are required to address these contemporary challenges. the solid-phase-based approach developed by flinders technology associates (fta) has been shown to be a powerful technology for the preparation of dna from different biological materials, including blood, saliva, plant t ...201424093923
efficacy evaluation of fungus syncephalastrum racemosum and nematicide avermectin against the root-knot nematode meloidogyne incognita on cucumber.the root-knot nematode (rkn) is one of the most damaging agricultural pests.effective biological control is need for controlling this destructive pathogen in organic farming system. during october 2010 to 2011, the nematicidal effects of the syncephalastrum racemosum fungus and the nematicide, avermectin, alone or combined were tested against the rkn (meloidogyne incognita) on cucumber under pot and field condition in china. under pot conditions, the application of s. racemosum alone or combined ...201424586982
isolation and characterization of a rhizobacterial antagonist of root-knot nematodes.the rhizobacterial strain jdm2 was isolated from the rhizosphere of the traditional chinese medicinal herb trichosanthes kirilowii in jiangsu province, china, and was identified as bacillus subtilis. exposure of cell-free filtrate of the strain to the root-knot nematode meloidogyne incognita under in vitro conditions caused substantial mortality of the second stage juvenile (j2) and significantly reduced egg hatchability. a greenhouse trial demonstrated that 56 days after treatment with jdm2, th ...201424465828
meloidogyne incognita - rice (oryza sativa) interaction: a new model system to study plant-root-knot nematode interactions in monocotyledons.plant-parasitic nematodes developed strategies to invade and colonize their host plants, including expression of immune suppressors to overcome host defenses. meloidogyne graminicola and m. incognita are root-knot nematode (rkn) species reported to damage rice (oryza sativa l.) cultivated in upland and irrigated systems. despite m. incognita wide host range, study of the molecular plant - rkn interaction has been so far limited to a few dicotyledonous model plants. the aim of this study was to i ...201426224554
apoplastic venom allergen-like proteins of cyst nematodes modulate the activation of basal plant innate immunity by cell surface receptors.despite causing considerable damage to host tissue during the onset of parasitism, nematodes establish remarkably persistent infections in both animals and plants. it is thought that an elaborate repertoire of effector proteins in nematode secretions suppresses damage-triggered immune responses of the host. however, the nature and mode of action of most immunomodulatory compounds in nematode secretions are not well understood. here, we show that venom allergen-like proteins of plant-parasitic ne ...201425500833
the tomato leucine-rich repeat receptor-like kinases slserk3a and slserk3b have overlapping functions in bacterial and nematode innate immunity.the somatic embryogenesis receptor kinase 3 (serk3)/brassinosteroid (br) insensitive 1-associated kinase 1 (bak1) is required for pattern-triggered immunity (pti) in arabidopsis thaliana and nicotiana benthamiana. tomato (solanum lycopersicum) has three slserk members. two of them exhibit particularly high levels of sequence similarity to atserk3 and, therefore, were named slserk3a and slserk3b. to characterize a role for slserk3a and slserk3b in defense, we suppressed each gene individually or ...201424675749
specific microbial attachment to root knot nematodes in suppressive soil.understanding the interactions of plant-parasitic nematodes with antagonistic soil microbes could provide opportunities for novel crop protection strategies. three arable soils were investigated for their suppressiveness against the root knot nematode meloidogyne hapla. for all three soils, m. hapla developed significantly fewer galls, egg masses, and eggs on tomato plants in unsterilized than in sterilized infested soil. egg numbers were reduced by up to 93%. this suggested suppression by soil ...201424532076
biological control of meloidogyne hapla using an antagonistic bacterium.we examined the efficacy of a bacterium for biocontrol of the root-knot nematode (rkn) meloidogyne hapla in carrot (daucus carota subsp. sativus) and tomato (solanum lycopersicum). among 542 bacterial isolates from various soils and plants, the highest nematode mortality was observed for treatments with isolate c1-7, which was identified as bacillus cereus based on cultural and morphological characteristics, the biolog program, and 16s rrna sequencing analyses. the population density and the nem ...201425289015
interspecific and host-related gene expression patterns in nematode-trapping fungi.nematode-trapping fungi are soil-living fungi that capture and kill nematodes using special hyphal structures called traps. they display a large diversity of trapping mechanisms and differ in their host preferences. to provide insights into the genetic basis for this variation, we compared the transcriptome expressed by three species of nematode-trapping fungi (arthrobotrys oligospora, monacrosporium cionopagum and arthrobotrys dactyloides, which use adhesive nets, adhesive branches or constrict ...201425384908
efficacy of root-associated fungi and pgpr on the growth of pisum sativum (cv. arkil) and reproduction of the root-knot nematode meloidogyne incognita.the effects of root-associated fungi (aspergillus awamori and glomus mosseae) and plant growth promoting rhizobacteria (pgpr) (pseudomonas putida, pseudomonas alcaligenes and paenibacillus polymyxa) were studied alone and in combination in glasshouse experiments on the growth of pea, enzyme activity (peroxidase and catalase) and reproduction of root-knot nematode meloidogyne incognita. application of a. awamori, g. mosseae and pgpr caused a significant increase in pea growth and enzyme activitie ...201322915081
characterization of thermo- and detergent stable antigenic glycosylated cysteine protease of euphorbia nivulia buch.-ham. and evaluation of its ecofriendly applications.an antigenic glycosylated cysteine protease has been purified from the latex of euphorbia nivulia buch.-ham. it exhibits remarkable protease activity in the presence of metal ions, oxidizing agents, organic solvents, and detergents. this enzyme showed potential role in leather processing industry due to its dehairing activity for animal hide without hydrolyzing fibrous proteins, producing, by this way, a better quality product. the enzyme can also be used for silver recovering from x-ray plates. ...201324348183
knocking-down meloidogyne incognita proteases by plant-delivered dsrna has negative pleiotropic effect on nematode vigor.the root-knot nematode meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. analysis of the m. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. several reports indicate that m. incognita proteases could play important roles in nematode parasitism, besides their function in ordinary digestion of giant cell contents for feeding. the precise roles of these proteins during parasitism however are still u ...201324392004
fatty acid-and retinol-binding protein, mj-far-1 induces tomato host susceptibility to root-knot nematodes.plant-parasitic nematodes produce at least one structurally unique class of small helix-rich retinol- and fatty-acid-binding proteins that have no counterparts in their plant hosts. herein we describe a protein of the plant-parasitic root-knot nematode meloidogyne javanica, which is a member of the nematode-specific fatty-acid- and retinol-binding (mj-far-1) family of proteins. the mj-far-1 mrna was detected through m. javanica pre-parasitic j2s, migratory and sedentary parasitic stages by quant ...201323717636
ectopic expression of atpad4 broadens resistance of soybean to soybean cyst and root-knot nematodes.the gene encoding pad4 (phytoalexin-deficient4) is required in arabidopsis for expression of several genes involved in the defense response to pseudomonas syringae pv. maculicola. atpad4 (arabidopsis thaliana pad4) encodes a lipase-like protein that plays a regulatory role mediating salicylic acid signaling.201323617694
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