Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
potential of synthetic endoperoxides against trichomonas vaginalis in vitro.metronidazole is well known for medicine against trichomonas vaginalis infection, but it has side effects though it is effective, and especially because reports of metronidazole-tolerant species are increasing, the development of new medicine is being required. here, we noticed the killing effects of endoperoxide compounds, n-89 and n-251 as new antimalarial drug candidates, on t. vaginalis and searched the possibility of development of new medicine. we added each of metronidazole, artemisinin, ...201728571765
c-reactive protein is differentially modulated by co-existing infections, vitamin deficiencies and maternal factors in pregnant and lactating indigenous panamanian women.the usefulness of c-reactive protein (crp) as a non-specific marker of inflammation during pregnancy and lactation is unclear in impoverished populations where co-existing infections and vitamin deficiencies are common.201728571565
strategies for prevention and treatment of trichomonas vaginalis infections.the last estimated annual incidence of trichomonas vaginalis worldwide exceeds that of chlamydia and gonorrhea combined. this critical review updates the state of the art on advances in t. vaginalis diagnostics and strategies for treatment and prevention of trichomoniasis. in particular, new data on treatment outcomes for topical administration of formulations are reviewed and discussed.201728539504
a community based study on pelvic inflammatory disease in postmenopausal females: microbiological spectrum and socio-demographic correlates.pelvic inflammatory disease (pid) is a common and serious complication of sexually transmitted diseases in young women but is rarely diagnosed in the postmenopausal women. very few studies have been done regarding post-menopausal pid.201728511414
interaction between trichomonas vaginalis and the prostate epithelium.most men infected with trichomonas vaginalis are asymptomatic and can remain undiagnosed and untreated. this has been hypothesized to result in chronic persistent prostatic infection. adhesion of the protozoan organisms to mucosal cells is considered a first and prerequisite step for t. vaginalis infection. adhesion of t. vaginalis to prostate epithelial cells has not yet been observed; however, there are several reports about inflammation of prostate epithelial cells induced by t. vaginalis. th ...201728506046
differential protein expressions in virus-infected and uninfected trichomonas vaginalis.protozoan viruses may influence the function and pathogenicity of the protozoa. trichomonas vaginalis is a parasitic protozoan that could contain a double stranded rna (dsrna) virus, t. vaginalis virus (tvv). however, there are few reports on the properties of the virus. to further determine variations in protein expression of t. vaginalis, we detected 2 strains of t. vaginalis; the virus-infected (v(+)) and uninfected (v(-)) isolates to examine differentially expressed proteins upon tvv infecti ...201728506033
glycogen synthase from the parabasalian parasite trichomonas vaginalis: an unusual member of the starch/glycogen synthase family.trichomonas vaginalis, a parasitic protist, is the causative agent of the common sexually-transmitted infection trichomoniasis. the organism has long been known to synthesize substantial glycogen as a storage polysaccharide, presumably mobilizing this compound during periods of carbohydrate limitation, such as might be encountered during transmission between hosts. however, little is known regarding the enzymes of glycogen metabolism in t. vaginalis. we had previously described the identificatio ...201728465215
immunocytochemistry improving the diagnosis of trichomonas vaginalis infections.the aim of this study was to evaluate the immunocytochemistry (icc) to diagnose trichomoniasis, particularly asymptomatic infections. by culture serial dilutions, icc was able to detect 1 trophozoite/ml, while the culture was positive up to 100 trophozoites/ml. the icc in vivo detection capability was assessed in vaginal secretions of mice experimentally infected and in vaginal swabs from asymptomatic hiv-positive pregnant women compared with culture. all vaginal secretion samples from mice were ...201728424786
expression of the enzymatically active legumain-like cysteine proteinase tvlegu-1 of trichomonas vaginalis in pichia pastoris.the legumain-like cysteine proteinase tvlegu-1 from trichomonas vaginalis plays a major role in trichomonal cytoadherence. however, its structure-function characterization has been limited by the lack of a reliable recombinant expression platform to produce this protein in its native folded conformation. tvlegu-1 has been expressed in escherichia coli as inclusion bodies and all efforts to refold it have failed. here, we describe the expression of the synthetic codon-optimized tvlegu-1 (tvlegu-1 ...201728419821
urinary meatal swabbing detects more men infected with mycoplasma genitalium and four other sexually transmitted infections than first catch urine.urinary meatal swabs compared with urine showed higher infection rates for mycoplasma genitalium (15.3% vs 12.6%, p = 0.035), chlamydia trachomatis (11.3% vs 9.3%, p = 0.039), neisseria gonorrhoeae (1.4% vs 1.1%, p = 1.00), trichomonas vaginalis (8.0% vs 1.7%, p < 0.001), and high-risk human papillomavirus (5.9% vs 3.4%, p = 0.078) respectively.201728703728
risk of bacterial vaginosis, trichomonas vaginalis and candida albicans infection among new users of combined hormonal contraception vs lng-ius.the study assessed the risk of bacterial vaginosis, trichomonas vaginalis and candida albicans infection among new users of either a combined oral contraceptive pill (coc) or the levonorgestrel-releasing intrauterine system (lng-ius).201728849960
parents' and teachers' views on sexual health education and screening for sexually transmitted infections among in-school adolescent girls in kenya: a qualitative study.to successfully develop and implement school-based sexual health interventions for adolescent girls, such as screening for chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis, it is important to understand parents' and teachers' attitudes towards sexual health education and acceptability of sexually transmitted infection (sti) screening interventions.201728806985
the detection of microorganisms related to urethritis from the oral cavity of male patients with urethritis.to investigate the presence of microorganisms related to urethritis in the oral cavity of male patients with urethritis and the efficacies of antimicrobials for urethritis on microorganisms in the oral cavity.201728803864
high prevalence of asymptomatic sexually transmitted infections among human immunodeficiency virus-infected pregnant women in a low-income south african community.there is a lack of evidence on the burden of chlamydia trachomatis (ct), neisseria gonorrhoeae (ng) and trichomonas vaginalis (tv) among hiv-infected pregnant women in south africa. we conducted a cross-sectional analysis of hiv-infected pregnant women in two healthcare facilities in a south african township to determine the prevalence of ct, ng and tv. hiv-infected pregnant women were recruited during the first antenatal care visit for their current pregnancy and requested to self-collect vulvo ...201728799824
cross-sectional study to evaluate trichomonas vaginalis positivity in women tested for neisseria gonorrhoeae and chlamydia trachomatis, attending genitourinary medicine and primary care clinics in bristol, south west england.highly sensitive, commercial nucleic acid amplification tests (naat) for trichomonas vaginalis have only recently been recommended for use in the uk. while testing for t. vaginalis is routine in symptomatic women attending genitourinary medicine (gum) clinics, it is rare in asymptomatic women or those attending primary care. the aim of this study was to evaluate the positivity of t. vaginalis using a commercial naat, in symptomatic and asymptomatic women undergoing testing for chlamydia and gono ...201728798195
prevalence and impact of sexually transmitted infections in pregnant women in central ethiopia.human immunodeficiency virus (hiv) continues to be a major global public health issue and omnipresent sexually transmitted infections (stis) increase the risk of hiv acquisition. moreover, stis and hiv in pregnant women can harm the unborn child. in this study, we systematically investigated the prevalence of hiv, relevant stis and vaginal group b streptococcus colonization among pregnant women presenting at asella teaching hospital in central ethiopia and their effect on perinatal mortality. a ...201728776463
sexually transmitted infections: a novel screening strategy for improving women's health in vulnerable populations.migrant women are one of the most vulnerable population to health problems and well-being. this study aimed at implementing a counseling and preventive strategy for sexually transmitted infections (stis) in undocumented migrant women in milan, italy.201728632191
burden and characteristics of hiv infection among female sex workers in kampala, uganda - a respondent-driven sampling survey.sex workers in uganda are at significant risk for hiv infection. we characterized the hiv epidemic among kampala female sex workers (fsw).201728601086
prevalence and risk factors associated with sexually transmitted infections (stis) among women of reproductive age in swaziland.sexually transmitted infections (stis) remain an important public health problem with approximately half a billion new cases annually among persons aged 15-49 years. epidemiological data on stis among women of reproductive age in swaziland are limited. the availability of epidemiological data on stis and associated risk factors in this population is essential for the development of successful prevention, diagnosis and management strategies in the country. the study aimed to determine the prevale ...201728559923
epidemiological investigation of the relationship between common lower genital tract infections and high-risk human papillomavirus infections among women in beijing, china.the incidence of lower genital tract infections in china has been increasing in recent years. the link between high-risk human papillomavirus (hr-hpv) and other sexually transmitted diseases (stds) remains unclear.201728531212
aerobic vaginitis: no longer a stranger.aerobic vaginitis (av) is the name given in 2002 to a vaginal infectious entity which was not recognized as such before. it is characterized by abnormal (dysbiotic) vaginal microflora containing aerobic, enteric bacteria, variable levels of vaginal inflammation and deficient epithelial maturation. although av and bacterial vaginosis (bv) share some characteristics, such as a diminished number or absence of lactobacilli, increased discharge (fishy smelling in bv, while in severe forms of av, a fo ...201728502874
prevalence and risk factors associated with stis among women initiating contraceptive implants in kingston, jamaica.there is limited information on rates of stis in jamaica due to syndromic management and limited aetiological surveillance. we examined the prevalence of chlamydia trachomatis (ct), neisseria gonorrhoeae (ng) and trichomonas vaginalis (tv) and characteristics associated with stis among sexually active women who participated in a randomised trial of a progestin implant initiation in jamaica (the sino-implant study (sis)).201728476913
evaluating the utility of syndromic case management for three sexually transmitted infections in women visiting hospitals in delhi, india.utility of syndromic case management (scm) in women visiting obstetrics & gynecology department needs to be evaluated as it is subjective and imperfect. consequently, antibiotic resistance has accelerated along with increased risk of infection to the partners. to understand the effectiveness and/or inadequacies of scm, 11000 women were recruited and examined by clinicians for infection by chlamydia trachomatis (ct), neisseria gonorrhoeae (ng), trichomonas vaginalis (tv), bacterial vaginosis (bv) ...201728469158
prevalence of human papillomavirus, chlamydia trachomatis, and trichomonas vaginalis infections in amazonian women with normal and abnormal cytology.sexually transmitted infections are an important cause of morbidity among sexually active women worldwide, and have been implicated as cofactors in the pathogenesis of cervical cancer. we investigated the prevalence of human papillomavirus (hpv), chlamydia trachomatis (ct), and trichomonas vaginalis (tv), and accessed the diversity of hpv in women with normal and abnormal cytology in manaus, brazil. we used polymerase chain reaction and hpv genotyping by direct sequencing. the chi-square test wa ...201728453175
proton pump inhibitors are associated with a reduced likelihood for sexually transmitted diseases in women in the emergency department.proton pump inhibitors (ppis) have been shown in cell culture to kill trichomonas vaginalis (tv) at lower half maximal inhibitory concentration values than metronidazole (flagyl), the most common medication used to treat the infection. however, there have been no previous clinical investigations to determine if ppis are associated with reduced risk for tv.201728442805
cervicovaginal levels of human β-defensin 1, 2, 3, and 4 of reproductive-aged women with chlamydia trachomatis infection.this study included women attending primary health care units in botucatu, são paulo, brazil, to assess the cervicovaginal levels of human β-defensin (hbd) 1, 2, 3, and 4 during chlamydia trachomatis infection.201728430679
profile of the triplex assay for detection of chlamydia, gonorrhea and trichomonas using the bd max™ system.chlamydia, gonorrhea and trichomonas are the most common curable sti. improved access to testing could reduce infection rates and prevent sequelae. nucleic acid amplification tests are the recommend class of diagnostic assay for these infections which are often asymptomatic. areas covered: a description of the bd max™ system (max) and the bd max ct/gc/tv assay is provided along with data from a large us clinical trial. the capacity of the system for other tests and for lab developed assays is al ...201728425774
dorsal longitudinal foreskin cut is associated with reduced risk of hiv, syphilis and genital herpes in men: a cross-sectional study in papua new guinea.various forms of penile foreskin cutting are practised in papua new guinea. in the context of an ecological association observed between hiv infection and the dorsal longitudinal foreskin cut, we undertook an investigation of this relationship at the individual level.201728406272
primary health clinic toilet/bathroom surface swab sampling can indicate community profile of sexually transmitted infections.the microbiome of built environment surfaces is impacted by the presence of humans. in this study, we tested the hypothesis that analysis of surface swabs from clinic toilet/bathroom yields results correlated with sexually transmitted infection (sti) notifications from corresponding human populations. we extended a previously reported study in which surfaces in toilet/bathroom facilities in primary health clinics in the australian northern territory (nt) were swabbed then tested for nucleic acid ...201728652937
ureaplasma urealyticum and u. parvum in sexually active women attending public health clinics in brazil.ureaplasma urealyticum and u. parvum have been associated with genital infections. the purpose of this study was to detect the presence of ureaplasmas and other sexually transmitted infections in sexually active women from brazil and relate these data to demographic and sexual health, and cytokines il-6 and il-1β. samples of cervical swab of 302 women were examined at the family health units in vitória da conquista. the frequency of detection by conventional pcr was 76·2% for mollicutes. in qpcr ...201728637523
prevalence and risk factors of chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis and other sexually transmissible infections among women attending antenatal clinics in three provinces in papua new guinea: a cross-sectional survey.background papua new guinea (png) is estimated to have among the highest prevalences of hiv and sexually transmissible infections (stis) of any asia-pacific country, and one of the highest burdens of maternal syphilis globally. the prevalence of curable stis, such as chlamydia trachomatis (ct), neisseria gonorrhoeae (ng) and trichomonas vaginalis (tv), among pregnant women in png is relatively unknown.201628636866
antiprotozoal activity of extracts of elaeodendron trichotomum (celastraceae).chagas disease, amebiasis, giardiasis and trichomoniasis represent a serious health problem in latin america. the drugs employed to treat these illnesses produce important side effects and resistant strains have appeared. the present study was aimed to evaluate the antiprotozoal activity of leaves, stem bark and root bark of elaeodendron trichotomum, a celastraceus, that is used in mexico as an anti-infective in febrile-type diseases.201628852732
sexually transmitted disease and male infertility: a systematic review.theoretically, sexually transmitted diseases (stds) have the potential to disrupt male fertility; however, the topic remains controversial.201628723470
[common genital infections].vulvo-vaginal infections are frequently encountered by gynecologists and general practitioners. the diagnosis and the recurrence of these affections must lead to a discussion about sexually transmitted disease, notably when trichomonas vaginalis and herpetic infections are found. even if they do not correspond to classical sexually transmitted diseases, their prevalence is increased by sexual intercourses reports and the number of partners which should also be treated. it is necessary to documen ...201628525226
pareto optimality in organelle energy metabolism analysis.in low and high eukaryotes, energy is collected or transformed in compartments, the organelles. the rich variety of size, characteristics, and density of the organelles makes it difficult to build a general picture. in this paper, we make use of the pareto-front analysis to investigate the optimization of energy metabolism in mitochondria and chloroplasts. using the pareto optimality principle, we compare models of organelle metabolism on the basis of single- and multiobjective optimization, app ...201624334395
genital trichomonas vaginalis is rare among female attendees at a sydney metropolitan sexual health clinic. 201626558330
exploring the mechanism of how tvmyb2 recognizes and binds ap65-1 by molecular dynamics simulations and free energy calculations.tvmyb2, one of the myb-like transcriptional factors in trichomonas vaginalis, binds to two closely spaced promoter sites, mre-1/mre-2r and mre-2f, on the ap65-1 gene. however, detailed dynamical structural characteristics of the tvmyb2-ap65-1 complex and a detailed study of the protein in the complex have not been done. focused on a specific tvmyb2-mre-2-13 complex (pdb code: ) and a series of mutants k51a, r84a and r87a, we applied molecular dynamics (md) simulation and molecular mechanics gene ...201626548411
trichomonas vaginalis: pathogenicity and potential role in human reproductive failure.trichomonas vaginalis, which colonizes the genitourinary tract of men and women, is a sexually transmitted parasite causing symptomatic or asymptomatic trichomoniasis. the host-parasite relationship is very complex, and clinical symptoms cannot likely be attributed to a single pathogenic effect. among the many factors responsible for interactions between t. vaginalis and host tissues, contact-dependent and contact-independent mechanisms are important in pathogenicity, as is the immune response.201626546373
five putative nucleoside triphosphate diphosphohydrolase genes are expressed in trichomonas vaginalis.trichomonas vaginalis is a protozoan that parasitizes the human urogenital tract causing trichomoniasis, the most common non-viral sexually transmitted disease. the parasite has unique genomic characteristics such as a large genome size and expanded gene families. ectonucleoside triphosphate diphosphohydrolase (e-ntpdase) is an enzyme responsible for hydrolyzing nucleoside tri- and diphosphates and has already been biochemically characterized in t. vaginalis. considering the important role of th ...201626590960
trichomonas vaginalis lipophosphoglycan exploits binding to galectin-1 and -3 to modulate epithelial immunity.trichomoniasis is the most common non-viral sexually transmitted infection caused by the vaginotropic extracellular protozoan parasite trichomonas vaginalis. the infection is recurrent, with no lasting immunity, often asymptomatic, and linked to pregnancy complications and risk of viral infection. the molecular mechanisms of immune evasion by the parasite are poorly understood. we demonstrate that galectin-1 and -3 are expressed by the human cervical and vaginal epithelial cells and act as patho ...201626589797
in vitro trichomonacidal activity and preliminary in silico chemometric studies of 5-nitroindazolin-3-one and 3-alkoxy-5-nitroindazole derivatives.a selection of 1,2-disubstituted 5-nitroindazolin-3-ones (1-19) and 3-alkoxy-5-nitroindazoles substituted at positions 1 (20-24) or 2 (25-39) from our in-house compound library were screened in vitro against the most common curable sexually transmitted pathogen, trichomonas vaginalis. a total of 41% of the studied molecules (16/39) achieved a significant activity of more than 85% growth inhibition at the highest concentration assayed (100 µg ml(-1)). among these compounds, 3-alkoxy-5-nitroindazo ...201626525913
trichomonas vaginalis erythrophagocytosis in the urine sediment. 201626468272
comparative aspects of immunity and vaccination in human and bovine trichomoniasis: a review.trichomonas vaginalis and tritrichomonas foetus are important extracellular protozoans that cause, respectively, human and bovine venereal diseases. trichomonads are extracellular parasites that primarily inhabit the genital tracts of the mammalian hosts where they overcome the mucus barrier and parasitize mucosa by contact-dependent or contact-independent cytotoxicity. transient immunity is usually achieved by the host after clinical infection. at present, vaccination in cattle reduces infectio ...201626424377
bifunctional activity of deoxyhypusine synthase/hydroxylase from trichomonas vaginalis.the trichomonas vaginalis genome analysis suggested the presence of a putative deoxyhypusine synthase (tvdhs) that catalyzes the posttranslational modification of eif-5a. herein, we expressed and purified the recombinant tvdhs (rtvdhs) protein (43 kda) and the recombinant tveif-5a (rtveif-5a) precursor protein (46 kda). a 41 kda band of the native tvdhs was recognized by western blot analysis in t. vaginalis total protein extract by a mouse polyclonal anti-rtvdhs antibody. the enzymatic activity ...201626410361
trichomoniasis in older individuals: a preliminary report from iran.infection with trichomonas vaginalis is one of the most common sexually transmitted diseases (stds) in humans. the prevalence of infection in iran has been reported 0.009-8 % depending on deferent socio-cultural conditions. this study aimed to determine the frequency of t. vaginalis according to age in patients referred to clinics, hospitals and medical diagnostic laboratories in karaj city, iran. for this purpose, fifty positive samples were collected from july 2012 to june 2014 from clinics, m ...201627876991
a preliminary evaluation of a new genexpert (gx) molecular point-of-care test for the detection of trichomonas vaginalis.global concerns regarding the prevalence, asymptomatic nature and burden of disease associated with trichomonas vaginalis (tv) continue. the lack of a portable molecular point-of-care assay to detect this infectious disease has meant that many remote or low-resource settings still need to rely on delayed results from central laboratories and/or syndromic management as treatment strategies. we evaluated the new genexpert (gx) tv nucleic acid amplification test (naat) compared with an in-house lab ...201626702132
design, synthesis and biological evaluation of chalconyl blended triazole allied organosilatranes as giardicidal and trichomonacidal agents.a series of chalconyl blended triazole allied silatranes (7a-g/8a-g/9a-g) were synthesized in good yields using a simple, economical and biocompatible synthetic route. the blend of three different pharmacologically active moieties into a single scaffold resulted into synergistic effect in their bio-activity. various substitutions were tried to study the structure activity relationship (sar) of the synthesized compounds on the basis of biological results. all the newly synthesized compounds were ...201626695730
novel structural features of the immunocompetent ceramide phospho-inositol glycan core from trichomonas vaginalis.the ceramide phosphoinositol glycan core (cpi-gc) of the lipophosphoglycan of trichomonas vaginalis is a major virulent factor of this common genitourinary parasite. while its carbohydrate composition has been reported before, its structure has remained largely unknown. we isolated the glycan portions of cpi-gc by nitrous acid deamination and hydrofluoric acid treatment and investigated their structures by methylation analysis and 1- and 2-d nmr. we found that the α-anomer of galactose is a majo ...201626671321
the past, present and future of fluorescent protein tags in anaerobic protozoan parasites.the world health organization currently recognizes diarrhoeal diseases as a significant cause of death in children globally. protozoan parasites such as giardia and entamoeba that thrive in the oxygen-deprived environment of the human gut are common etiological agents of diarrhoea. in the urogenital tract of humans, the anaerobic protozoan parasite trichomonas vaginalis is notorious as the most common non-viral, sexually transmitted pathogen. even with high medical impact, our understanding of a ...201626653973
recent advances in the trichomonas vaginalis field.the microaerophilic protist parasite trichomonas vaginalis is occurring globally and causes infections in the urogenital tract in humans, a condition termed trichomoniasis. in fact, trichomoniasis is the most prevalent non-viral sexually transmitted disease with more than 250 million people infected every year. although trichomoniasis is not life threatening in itself, it can be debilitating and increases the risk of adverse pregnancy outcomes, hiv infection, and, possibly, neoplasias in the pro ...201626918168
comparison of permanent staining methods for the laboratory diagnosis of trichomoniasis.trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease (std) in the world. the diagnosis is based on wet mount preparation and direct microscopy on fixed and stained clinical specimens. the aim of this study was to compare the performance of different fixing and staining techniques used in the detection of t. vaginalis in urine. the smears were fixed and submitted to different methods of permanent staining and then, the morphologica ...201626910452
hiv risk and behaviour among part-time versus professional fsw: baseline report of an interventional cohort in burkina faso.to readjust hiv control programmes in africa, we assessed the factors associated with high-risk behaviours and hiv infection among young female sex workers (fsw) in burkina faso.201626834073
inflammatory response of a prostate stromal cell line induced by trichomonas vaginalis.while trichomonas vaginalis, a cause of sexually transmitted infection, is known as a surface-dwelling protozoa, trichomonads have been detected in prostatic tissue from benign prostatic hyperplasia and prostatitis by immunoperoxidase assay or pcr. however, the immune response of prostate stromal cells infected with t. vaginalis has not been investigated. our objective was to investigate whether t. vaginalis could induce an inflammatory response in prostate stromal cells. incubation of a human p ...201626832322
a new strategy for trichomonas testing female adolescents in the emergency department.sensitive trichomonas diagnostic testing has become available, including nucleic acid amplification tests (naats) and a rapid antigen test. the study purpose was to determine if adding sensitive trichomonas testing to routine female sexually transmitted infection (sti) evaluations would increase trichomonas identification and treatment.201626820440
trichomonas vaginalis infection and risk of advanced prostate cancer.the epidemiologic evidence for an association of trichomonas vaginalis (tv) with overall prostate cancer is mixed, but some studies suggest tv may increase risk of more aggressive disease. the aim of this study was to assess whether tv serostatus is associated with advanced or fatal prostate cancer.201626818005
osmc and incomplete glycine decarboxylase complex mediate reductive detoxification of peroxides in hydrogenosomes of trichomonas vaginalis.osmotically inducible protein (osmc) and organic hydroperoxide resistance protein (ohr) are small, thiol-dependent peroxidases that comprise a family of prokaryotic protective proteins central to the defense against deleterious effects of organic hydroperoxides, which are reactive molecules that are formed during interactions between the host immune system and pathogens. trichomonas vaginalis, a sexually transmitted parasite of humans, possesses osmc homologues in its hydrogenosomes, anaerobic m ...201626794804
trichomonas vaginalis: an updated overview towards diagnostic improvement.the protozoan trichomonas vaginalis (tv) is responsible for trichomonosis, a sexually transmitted disease (std) with a significant incidence worldwide. this infection is one of the most common non-viral stds, representing almost 50% of all curable stds. trichomonosis has an incidence of 180 million new cases worldwide. nowadays, the 'gold standard' for tv diagnosis remains the use of in vitro cultures combined with daily visual microscopic evaluations, which is a time-consuming and low sensitive ...201626751868
amaurocine: anti-trichomonas vaginalis protein produced by the basidiomycete amauroderma camerarium.trichomonas vaginalis is the causative agent of trichomoniasis, the most common nonviral std worldwide. this infection can lead to severe health conditions, especially when women are affected. metronidazole and tinidazole are the only choices of treatment. in this sense, natural bioactive compounds against t. vaginalis are an interesting approach in the search for more efficient therapies. herein, amaurocine, a 12 kda protein, produced by the mushroom amauroderma camerarium was purified and test ...201626706604
a prospective study of trichomonas vaginalis and prostate cancer risk among african american men.african americans (aa) have a higher prevalence of trichomonas vaginalis (tv) infection and a higher prostate (pc) risk. past studies suggest an association between tv seropositivity and pc, and therefore we prospectively investigated this association among aa men.201627091219
role of disulfide linkage in action of bis(dialkylaminethiocarbonyl)disulfides as potent double-edged microbicidal spermicide: design, synthesis and biology.trichomoniasis and candidiasis are amongst the most common morbidity-causing reproductive tract infections, generally treated by metronidazole and fluconazole respectively. poor vaginal efficacy, drug-resistance and non-spermicidal nature limit their use as topical microbicidal contraceptives. bis(dialkylaminethiocarbonyl)disulfides (4-38) were designed as dually active, non-surfactant molecules capable of eliminating trichomonas vaginalis and candida strains as well as irreversibly immobilizing ...201627084496
evaluation of the in vitro activity of ceragenins against trichomonas vaginalis.trichomonosis, caused by the protozoan parasite trichomonas vaginalis, is a curable sexually transmitted disease that is most commonly encountered worldwide. increasing importance of trichomoniasis and emerging of resistance against metronidazole lead to search for alternative drugs with different mode of activity. the purpose of this study was to determine in vitro activity of ceragenins (csa-13, csa-44, csa-13, and csa-138) against the metronidazole-susceptible (atcc 30001) and metronidazole-r ...201627078662
are the fatty acids responsible for the higher effect of oil and alcoholic extract of nigella sativa over its aqueous extract on trichomonas vaginalis trophozoites?trichomoniasis, the disease caused by the flagellate protozoan trichomonas vaginalis is the sexually transmitted infection with the largest annual incidence. metronidazole is the drug of choice recommended for the treatment of human trichomoniasis but it can lead to drug resistance and many other adverse effects. so, it is necessary for new alternatives for the treatment of this infection. medicinal plants or herbs could be good alternative regimens to be inexpensive, effective and safe to use. ...201627065592
caatinga plants: natural and semi-synthetic compounds potentially active against trichomonas vaginalis.trichomonas vaginalis causes trichomoniasis; the most common but overlooked non-viral sexually transmitted disease worldwide. the treatment is based at 5'-nitroimidazoles, however, failure are related to resistance of t. vaginalis to chemotherapy. caatinga is a uniquely brazilian region representing a biome with type desert vegetation and plants present diverse biological activity, however, with few studies. the aim of this study was to investigate the activity against t. vaginalis of different ...201627020521
antichagasic and trichomonacidal activity of 1-substituted 2-benzyl-5-nitroindazolin-3-ones and 3-alkoxy-2-benzyl-5-nitro-2h-indazoles.two series of new 5-nitroindazole derivatives, 1-substituted 2-benzylindazolin-3-ones (6-29, series a) and 3-alkoxy-2-benzyl-2h-indazoles (30-37, series b), containing differently functionalized chains at position 1 and 3, respectively, have been synthesized starting from 2-benzyl-5-nitroindazolin-3-one 5, and evaluated against the protozoan parasites trypanosoma cruzi and trichomonas vaginalis, etiological agents of chagas disease and trichomonosis, respectively. many indazolinones of series a ...201627017556
trichomonas vaginalis induces il-1β production in a human prostate epithelial cell line by activating the nlrp3 inflammasome via reactive oxygen species and potassium ion efflux.trichomonas vaginalis is a sexually transmitted protozoan parasite that causes vaginitis in women, and urethritis and prostatitis in men. il-1β is synthesized as immature pro-il-1β, which is cleaved by activated caspase-1. caspase-1 is, in turn, activated by a multi-protein complex known as an inflammasome. in this study, we investigated the inflammatory response of a prostate epithelial cell line (rwpe-1) to t. vaginalis and, specifically, the capacity of t. vaginalis to activate the nlrp3 infl ...201626959386
influence of 120 kda pyruvate:ferredoxin oxidoreductase on pathogenicity of trichomonas vaginalis.trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. however, the exact mechanism was not clarified. in this study, the author investigated whether the 120 kda protein of t. vaginalis may be involved in pathogenicity of trichomonads. antibodies against 120 kda protein of t. vaginalis, which was identifie ...201626951982
correlation between trichomonas vaginalis and concurrency: an ecological study.objective. there is a large variation in the prevalence of trichomonas vaginalis (tv) between different countries and between racial groups within countries. sexual partner concurrency may play a role. we investigate the correlation between the prevalence of sexual partner concurrency and tv prevalence. methods. spearman's correlation to assess relationship between tv prevalence in women and point prevalence of concurrency in men in (1) 11 countries with comparable data (concurrency data from wh ...201626949392
trichomonas vaginalis ntpdase and ecto-5'-nucleotidase hydrolyze guanine nucleotides and increase extracellular guanosine levels under serum restriction.trichomonas vaginalis is the aethiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease in the world. the purinergic signaling pathway is mediated by extracellular nucleotides and nucleosides that are involved in many biological effects as neurotransmission, immunomodulation and inflammation. extracellular nucleotides can be hydrolyzed by a family of enzymes known as ectonucleotidases including the ecto-nucleoside triphosphate diphosphohydrolases (e-ntpdases) fam ...201627150347
trichomonas vaginalis repair of iron centres proteins: the different role of two paralogs.trichomonas vaginalis, the causative parasite of one of the most prevalent sexually transmitted diseases is, so far, the only protozoan encoding two putative repair of iron centres (ric) proteins. homologs of these proteins have been shown to protect bacteria from the chemical stress imposed by mammalian immunity. in this work, the biochemical and functional characterisation of the t. vaginalis rics revealed that the two proteins have different properties. expression of ric1 is induced by nitros ...201627124376
structural investigation and inhibitory response of halide on phosphoserine aminotransferase from trichomonas vaginalis.phosphoserine aminotransferase (psat) catalyses the second reversible step of the phosphoserine biosynthetic pathway in trichomonas vaginalis, which is crucial for the synthesis of serine and cysteine.201627102280
recombinant trichomonas vaginalis eif-5a protein expressed from a eukaryotic system binds specifically to mammalian and putative trichomonal eif-5a response elements (eres).trichomonas vaginalis eif-5a-like protein (tveif-5a) belongs to the highly conserved eif-5a family of proteins that contains a unique polyamine-derived amino acid, hypusine. recently, we determined that the polyamine putrescine is required for tveif-5a mrna stability, and it is necessary for stability and maturation of the tveif-5a protein. eukaryotic eif-5a is known to be involved in mrna turnover and is capable of sequence-specific rna binding to eif-5a response elements (eres). these ere sequ ...201627620329
sending a message: extracellular vesicles of pathogenic protozoan parasites.parasitic unicellular eukaryotes use extracellular vesicles (evs) as vehicles for intercellular communication and host manipulation. by using various mechanisms to generate evs and by transferring a wide range of molecules through evs, pathogenic protozoans are able to establish infective niches, modulate the immune system of the host and cause disease. in addition to effects on the host, evs are able to transfer virulence factors, drug-resistance genes and differentiation factors between parasi ...201627615028
trichomonas vaginalis: a possible foe to prostate cancer.prostate cancer (pca) is the most common malignancy in men in usa, and the role of trichomonas vaginalis (t. vag) in the development of pca is still controversial. clonogenic assay, pcna staining, tunel staining and caspase-3 activity assay were used to investigate the in vitro role of t. vag in human prostate cancer. we further investigated the possible molecular mechanisms using rt-pcr and immunohistochemical staining. culture supernatant of t. vag inhibits growth of pc-3 prostate cancer cells ...201627613161
effect of nigella sativa alcoholic extract and oil, as well as phaseolus vulgaris (kidney bean) lectin on the ultrastructure of trichomonas vaginalis trophozoites.trichomonas vaginalis is a parasitic protozoan that is the aetiological agent of trichomoniasis, the most common non-viral sexually transmitted disease worldwide. currently, the compound of choice for the treatment of t. vaginalis infections is metronidazole, however, it has many side effects and an increase in metronidazole-resistant trichomoniasis has been observed. medicinal plants could be a source of new antiprotozoal drugs with high activity, low toxicity and lower price. the present work ...201627605771
a calcium sensor - protein kinase signaling module diversified in plants and is retained in all lineages of bikonta species.calcium (ca(2+)) signaling is a universal mechanism of signal transduction and involves ca(2+) signal formation and decoding of information by ca(2+) binding proteins. calcineurin b-like proteins (cbls), which upon ca(2+) binding activate cbl-interacting protein kinases (cipks) regulate a multitude of physiological processes in plants. here, we combine phylogenomics and functional analyses to investigate the occurrence and structural conservation of cbl and cipk proteins in 26 species representi ...201627538881
purification and identification of amylases released by the human pathogen trichomonas vaginalis that are active towards glycogen.the parasitic protist trichomonas vaginalis is the causative agent of the sexually transmitted infection trichomoniasis. in the laboratory, t. vaginalis is typically cultured in a serum-containing medium with maltose or glucose as the carbon source. the nature of the carbohydrates used by the organism in the environment of its host is unclear. however, the vagina contains substantial amounts of glycogen, which is believed to provide a growth substrate for the vaginal microbiota. we have shown pr ...201627506885
self-testing for trichomonas vaginalis at home using a point-of-care test by women who request kits via the internet.we offered a point-of-care test for trichomonas vaginalis to women via the internet to determine if it was acceptable to women to perform the test at home. most of the 102 participants felt that it was easy to collect the specimen, follow the instructions, and read and interpret the results for the trichomonas self-testing assay.201627491592
the glycolytic enzyme triosephosphate isomerase of trichomonas vaginalis is a surface-associated protein induced by glucose that functions as a laminin- and fibronectin-binding protein.triosephosphate isomerase of trichomonas vaginalis (tvtim) is a 27-kda cytoplasmic protein encoded by two genes, tvtim1 and tvtim2, that participates in glucose metabolism. tvtim is also localized to the parasite surface. thus, the goal of this study was to identify the novel functions of the surface-associated tvtim in t. vaginalis and to assess the effect of glucose as an environmental factor that regulates its expression and localization. reverse transcription-pcr (rt-pcr) showed that the tvt ...201627481251
molecular sensitivity threshold of wet mount and an immunochromatographic assay evaluated by quantitative real-time pcr for diagnosis of trichomonas vaginalis infection in a low-risk population of childbearing women.vaginal trichomoniasis is a sexually transmitted infection caused by trichomonas vaginalis, a flagellated protozoan. diagnosis of t. vaginalis infection is mainly performed by wet mount microscopy, with a sensitivity ranging from 38% to 82%, compared to culture, still considered the gold standard. commercial immunochromatographic tests for monoclonal-antibody-based detection have been introduced as alternative methods for diagnosis of t. vaginalis infection and have been reported in some studies ...201627367320
morphological changes of trichomonas vaginalis treated by ornidazole: an in-vitro study.nitroimidazoles, which are drugs that are used to effectively treat trichomonas vaginalis, alter the structure of the t. vaginalis cell membrane, penetrate into its cytoplasm and nucleus and block cellular metabolism. in this study, we observed the morphological changes that occurred in t. vaginalis during in vitro exposure to 1.3 μg/ml of ornidazole at various time intervals ranging from 10 minutes to 10 hours.201627349003
aggregation kinetic dataset to determine the stability of the purified and refolded recombinant pptvcp4 protein of trichomonas vaginalis.the recombinant pptvcp4 (pptvcp4r) protein, a specific inhibitor of the proteolytic activity and virulence properties of trichomonas vaginalis, depending on cathepsin l-like cysteine proteinases (cps) (http:dx.doi.org/ 10.1016/j.biocel.2014.12.001[1], http:dx.doi.org/ 10.1016/j.micinf.2013.09.002[2], http:dx.doi.org/ 10.1155/2015/946787[3]) was stable in the elution buffer up to two months at 4 °c. however, it was prone to aggregate in pbs (functional assay buffer) [1]. therefore, before functio ...201627331109
proliferation of prostate stromal cell induced by benign prostatic hyperplasia epithelial cell stimulated with trichomonas vaginalis via crosstalk with mast cell.chronic inflammation has a role in the pathogenesis of benign prostatic hyperplasia (bph) and prostate cancer. mast cells have been detected in chronic inflammatory infiltrate of the prostate, and it is possible that the interaction between prostate epithelial cells and trichomonas vaginalis influences the activity of mast cells in the prostate stroma. activated mast cells might influence the biological functions of nearby tissues and cells. in this study, we investigated whether mast cells reac ...201627325623
comparative protein structure modeling using modeller.comparative protein structure modeling predicts the three-dimensional structure of a given protein sequence (target) based primarily on its alignment to one or more proteins of known structure (templates). the prediction process consists of fold assignment, target-template alignment, model building, and model evaluation. this unit describes how to calculate comparative models using the program modeller and how to use the modbase database of such models, and discusses all four steps of comparativ ...201627322406
a molecular-beacon-based asymmetric pcr assay for easy visualization of amplicons in the diagnosis of trichomoniasis.the currently available nucleic acid amplification tests (naats) for trichomoniasis are accurate, quick and confirmative with superior sensitivity than traditional culture-based microbiology assays. however, these assays are associated with problems of carry over contamination, false positive results, requirement of technical expertise for performance and detection of end product. hence, a diagnostic assay with easy visualization of the amplified product will be profitable. an in-house, rapid, s ...201627318568
molecular characterization of double-stranded rna virus in trichomonas vaginalis egyptian isolates and its association with pathogenicity.trichomoniasis is a common human sexually transmitted infection caused by trichomonas vaginalis. the parasite can be infected with double-stranded rna viruses (tvv). this viral infection may have important implications on trichomonal virulence and disease pathogenesis. this study aimed to determine the prevalence of t. vaginalis virus among isolates obtained from infected (symptomatic and asymptomatic) women in ismailia city, egypt, and to correlate the virus-infected isolates with the clinical ...201627316695
maldi-tof mass spectrometry as a potential tool for trichomonas vaginalis identification.trichomonas vaginalis is a flagellated protozoan causing trichomoniasis, a sexually transmitted human infection, with around 276.4 million new cases estimated by world health organization. culture is the gold standard method for the diagnosis of t. vaginalis infection. recently, immunochromatographic assays as well as pcr assays for the detection of t. vaginalis antigen or dna, respectively, have been also available. although the well-known genome sequence of t. vaginalis has made possible the a ...201627282151
novel hydrogenosomes in the microaerophilic jakobid stygiella incarcerata.mitochondrion-related organelles (mros) have arisen independently in a wide range of anaerobic protist lineages. only a few of these organelles and their functions have been investigated in detail, and most of what is known about mros comes from studies of parasitic organisms such as the parabasalid trichomonas vaginalis here, we describe the mro of a free-living anaerobic jakobid excavate, stygiella incarcerata we report an rnaseq-based reconstruction of s. incarcerata's mro proteome, with an a ...201627280585
in response to 'successful treatment of refractory trichomonas vaginalis infection using intravenous metronidazole' by hawkins et al. 201627278450
evaluation of presto(plus) assay and lightmix kit trichomonas vaginalis assay for detection of trichomonas vaginalis in dry vaginal swabs.this is an evaluation study of the presto(plus) assay for t. vaginalis by comparing to the tib molbiol lightmix kit trichomonas vaginalis assay using 615 dry collected vaginal and rectal swabs. discordant samples were analyzed by the qiagen® microbial dna qpcr for tv assay. both assays showed comparable performances (mcnemar p>0.05).201627268968
rapid diagnosis of trichomonas vaginalis by testing vaginal swabs in an isothermal helicase-dependent amplivue assay.the amplivue trichomonas assay (quidel) is a new federal drug administration-cleared rapid test for qualitative detection of trichomonas vaginalis (tv) dna in female vaginal specimens. the assay is based on biohelix's helicase-dependent amplification isothermal technology in conjunction with a disposable lateral-flow detection device, with a total turnaround time of approximately 45 minutes.201627196258
optimization of trichomonas vaginalis diagnosis during pregnancy at a university hospital, argentina.the aim of this study was to evaluate different methods for trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. a total of 386 vaginal exudates from pregnant women were analyzed. t. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged may-grunwald giemsa (mgg) staining, and wet mount with sodium-acetate-formalin (saf)/methylene blue method. pcr for 18s rrna gene as wel ...201627180578
prevalence of trichomoniasis by pcr in women attending health screening in korea.trichomoniasis is the most common curable sexually-transmitted infection (sti) worldwide. there are few reports on the prevalence of trichomonas vaginalis in korea. the purpose of this study was to examine the prevalence of trichomoniasis by pcr in guri city, korea. all adult women who visited hanyang university guri hospital for health screening within the national health care service were invited to participate in the study, and 424 women were enrolled between march and june 2011. pcr was used ...201627180577
inflammatory responses in a benign prostatic hyperplasia epithelial cell line (bph-1) infected with trichomonas vaginalis.trichomonas vaginalis causes the most prevalent sexually transmitted infection worldwide. trichomonads have been detected in prostatic tissues from prostatitis, benign prostatic hyperplasia (bph), and prostate cancer. chronic prostatic inflammation is known as a risk factor for prostate enlargement, benign prostatic hyperplasia symptoms, and acute urinary retention. our aim was to investigate whether t. vaginalis could induce inflammatory responses in cells of a benign prostatic hyperplasia epit ...201627180569
comparative inhibition study of compounds identified in the methanolic extract of apamarga kshara against trichomonas vaginalis carbamate kinase (tvck): an enzoinformatics approach.in the present study, we have identified ten compounds, namely dodecanol acid, myristic acid, neophytadiene, palmitic acid, heptadecanoic acid, linoleic acid, elaidic acid, 3-7-dimethyl acid, stearic acid and methyl eicos acid, of the methanolic extract of apamarga kshara by gc-ms analysis. apamarga kshara has been reported to be active against cervical erosion. major causal organism for cervical erosion is trichomonas vaginalis. however, there is a paucity of information about the mechanism of ...201626310620
a case of metronidazole-resistant trichomonas vaginalis in pregnancy.trichomonas vaginalis is a sexually transmitted protozoan infection resulting in vulvo-vaginitis and altered vaginal discharge in symptomatic women. trichomoniasis has been implicated in causing adverse pregnancy outcomes such as low birth weight and pre-term labour. metronidazole is the recommended first-line treatment for trichomonal infection. other nitroimidazoles, such as tinidazole, are used as alternative regimens with similar activity but at a greater expense. treatment failure usually r ...201626384945
trichomonas vaginalis infection among homeless and unstably housed adult women living in a resource-rich urban environment.the social context of poverty is consistently linked to trichomonas vaginalis infection, yet few studies regarding t. vaginalis have been conducted exclusively among low-income individuals. we identified social determinants of health associated with prevalent t. vaginalis infection among homeless and unstably housed adult women.201626347544
eicosapentaenoic acid modulates trichomonas vaginalis activity.trichomonas vaginalis is a sexually transmitted parasite and, while it is often asymptomatic in males, the parasite is associated with disease in both sexes. metronidazole is an effective treatment for trichomoniasis, but resistant strains have evolved and, thus, it has become necessary to investigate other possible therapies. in this study, we examined the effects of native and oxidized forms of the sodium salts of eicosapentaenoic, docosahexaenoic, and arachidonic acids on t. vaginalis activit ...201626333486
anti-trichomonas vaginalis properties of the oil of amomum tsao-ko and its major component, geraniol.trichomonosis, caused by the flagellate protozoan trichomonas vaginalis, is the most common non-viral sexually transmitted disease (std) and 5-nitroimidazole drugs are used for the treatment. however, a growing number of t. vaginalis isolates are resistant to these drugs, which make it becomes an urgent issue.201625963227
management of recalcitrant trichomonas vaginalis in pregnancy: a case report.a case report of a pregnant woman with recalcitrant trichomonas vaginalis is described. this case was managed with suppressive treatment with metronidazole during pregnancy and cleared with paromomycin vaginal treatment after delivery.201625829518
the secretory products of trichomonas vaginalis decrease fertilizing capacity of mice sperm in vitro.trichomonas vaginalis infection is one of the most prevalent sexually transmitted infections in humans and is now recognized as an important cause of infertility in men. there is little information about the effect of extracellular polymeric substances (eps) from t. vaginalis on sperm, but previous reports do not provide a conclusive description of the functional integrity of the sperm. to investigate the impact of eps on the fertilizing capacity of sperm, we assessed sperm motility, acrosomal s ...201625578937
Refresh
firstprev
12345678910...
nextlast
Displaying items 101 - 200 of 4838