Publications

pyrene is metabolized to bound residues by penicillium janthinellum sfu403.	we have previously shown that the filamentous fungus, penicillium janthinellum sfu403 (sfu403) oxidizes pyrene to pyrene 1,6- and 1,8-quinones and that the level of pyrenequinones (pqs) subsequently declines suggesting that pqs are not terminal metabolites. the purpose of this study was to determine the fate of pqs in sfu403. first, we compared the fate of 14c-pyrene in sfu403 and a non-pyrene-oxidizing fungus, a paecilomyces sp.. after 7 days of incubation, more than 80% of the radioactivity wa ...	2000	11487060
degradation and mineralization of high-molecular-weight polycyclic aromatic hydrocarbons by defined fungal-bacterial cocultures.	this study investigated the biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons (pahs) in liquid media and soil by bacteria (stenotrophomonas maltophilia vun 10,010 and bacterial consortium vun 10,009) and a fungus (penicillium janthinellum vuo 10, 201) that were isolated from separate creosote- and manufactured-gas plant-contaminated soils. the bacteria could use pyrene as their sole carbon and energy source in a basal salts medium (bsm) and mineralized significant amounts ...	2000	10698765
penicillopepsin-jt2, a recombinant enzyme from penicillium janthinellum and the contribution of a hydrogen bond in subsite s3 to k(cat).	the nucleotide sequence of the gene (pepa) of a zymogen of an aspartic proteinase from penicillium janthinellum with a 71% identity in the deduced amino acid sequence to penicillopepsin (which we propose to call penicillopepsin-jt1) has been determined. the gene consists of 60 codons for a putative leader sequence of 20 amino acid residues, a sequence of about 150 nucleotides that probably codes for an activation peptide and a sequence with two introns that codes for the active aspartic proteina ...	2000	10850809
isolation and characterization of acid- and al-tolerant microorganisms.	acid- and aluminum (al)-tolerant microorganisms were isolated from tea fields, from which six strains were selected and identified as cryptococcus humicola, rhodotorula glutinis, aspergillus flavus link, penicillium sp., penicillium janthinellum biourge and trichoderma asperellum. they were tolerant to al up to 100-200 mm and could grow at low ph, 2.5-2.2. in a glucose medium (ph 3.5) the ph of the spent medium decreased to below 3.0. the toxic inorganic monomeric al in the spent medium decrease ...	2000	10930728
beta-xylosidase recovery by reversed micelles. use of cationic surfactant.	beta-xylosidase, an enzyme produced by penicillium janthinellum fungus, was prepurified by fractionated precipitation with ethanol and extracted by reversed micelles of n-benzyl-n-dodecyl-n-bis(2-hydroxyethyl) ammonium chloride (bdbac) cationic surfactant. a 2(5-1) fractional factorial design was employed to evaluate the influence of the following factors on the enzyme extraction: ph (a), conductivity (b), surfactant concentration (c), cosolvent concentration (d) and temperature (e). a statistic ...	2000	10849861
in vitro expression of penicillium janthinellum cellobiohydrolase i gene in a coupled transcription-translation system.	an enzymatically-active fungal cellobiohydrolase i (cbh i) was first synthesized in a coupled reticulocyte lysate system lacking of glycosylation modification by the template dna(cbh1) in the presence of t7 rna polymerase. the synthesized cbh i had the expected size (57 kda) and catalyzed the substrate of p-nitrophenyl-beta-d-cellobioside (pnpc), and had no activity against carboxymethyl cellulose (cmc-na). the k(m) and v(max) values of the cbh i for pnpc were 0.82 mmol and 0.067 micromol min(-1 ...	2000	10989180
partitioning of xylanolitic complex from penicillium janthinellum by an aqueous two-phase system.	this work evaluates the influences of five parameters (ph, peg molecular mass, peg concentration, concentration of buffer k2hpo4-kh2po4 and nacl concentration) on xylanolitic complex partitioning produced by p. janthinellum in aqueous two-phase systems, using a 2(5) factorial experimental design. a mathematical model to quantify the influence of these parameters was attained and statistically tested. the optimum point for total protein extraction was obtained under the following conditions: ph 7 ...	2000	10942304
inactivation of mucor plumbeus by the combined actions of chitinase and high hydrostatic pressure.	sporangiospores were treated with high hydrostatic pressure and/or fungal chitinase in order to study the inhibition of germination and growth of the food spoiling mold mucor plumbeus. total fungal inhibition was obtained either at 4.0 kbar or by 10 u/ml of chitinase from penicillium janthinellum. a pretreatment with 1 u/ml of the same chitinase reduced the pressure necessary to obtain complete spore inhibition to 3 kbar.	1999	10573398
optimization of pyrene oxidation by penicillium janthinellum using response-surface methodology.	at present, there is little information on the optimization of the degradation of polycyclic aromatic hydrocarbons (pah) by deuteromycete filamentous fungi, a reaction catalyzed by cytochrome p450 monooxygenases. we utilized response-surface methodology to determine the optimal growth conditions for the oxidation of the pah pyrene by penicillium janthinellum sfu403, with respect to the variables glucose concentration, nitrate concentration and bioconversion time. models were derived for the rela ...	1999	10341435
subcellular localization of fructosyl amino acid oxidases in peroxisomes of aspergillus terreus and penicillium janthinellum.	fructosyl amino acid oxidase (faod) is the enzyme catalyzing the oxidative deglycation of amadori compounds, such as fructosyl amino acids, yielding the corresponding amino acids, glucosone, and h(2)o(2). in a previous report, we determined the primary structures of cdnas coding for faods from two fungal strains aspergillus terreus ap1 and penicillium janthinellum and we found that both fungal faods included the putative peroxisome targeting signal 1 (pts1) at the carboxyl terminal (yoshida, n. ...	1999	16232435
[complete amino acid sequence of catalase from the fungus penicillium vitale].	the polypeptide sequence of the unmodified catalase from penicillium vitale containing 696 amino acid residues was deduced. the sequences of 76 tryptic peptides of the unmodified catalase, 63 tryptic peptides of the catalase with modified lys residues, 48 peptides resulting from catalase cleavage by the staphylococcus aureus v8 protease, and 9 fragments obtained by brcn-treatment were considered, and a comparison with the sequences of other catalases was made.	1998	9612556
the amino acid sequences of carboxypeptidases i and ii from aspergillus niger and their stability in the presence of divalent cations.	the amino acid sequences of serine carboxypeptidase i (cpd-i) and ii (cpd-ii), respectively, from aspergillus niger have been determined by conventional edman degradation of the reduced and vinylpyridinated enzymes and peptides hereof generated by cleavage with cyanogen bromide, iodobenzoic acid, glutamic acid cleaving enzyme, aspn-endoproteinase and endolysc proteinase. cpd-i consists of a single peptide chain of 471 amino acid residues, three disulfide bridges and nine n-glycosylated asparagin ...	1998	9748653
xylanase recovery by ethanol and na2so4 precipitation.	xylans are the major components of the hemicellulosic fraction of lignocellulosic biomass and their hydrolysis can be obtained using xylanases from penicillium janthinellum. in this work, sugarcane bagasse hemicellulosic hydrolysate was used as the substrate for producing xylanase. the precipitation of these enzymes was studied using ethanol and na2so4 as precipitating agents. ethanol precipitation experiments were performed batchwise in concentrations ranging from 10 to 80%, ph 4.0 to 7.0, at 4 ...	1998	18576030
xylanase recovery. effect of extraction conditions on the aqueous two-phase system using experimental design.	the partitioning of xylanase produced by penicillium janthinellum in aqueous two-phase systems (atps) using poly(ethylene glycol) (peg) and phosphate (k2hpo4/kh2po4) was studied employing a statistical experimental design. the aim was to identify the key factors governing xylanase partitioning. the interactions of five factors (peg concentration molecular weight, concentration of buffer k2hpo4/kh2po4, ph, and nacl concentration) and their main effects on the partition coefficient (k) were evalua ...	1998	18576027
penicillium janthinellum in sputum and bronchoalveolar lavage in an aids patient with pneumonia.		1997	11864114
structure of the heme d of penicillium vitale and escherichia coli catalases.	a heme d prosthetic group with the configuration of a cis-hydroxychlorin gamma-spirolactone has been found in the crystal structures of penicillium vitale catalase and escherichia coli catalase hydroperoxidase ii (hpii). the absolute stereochemistry of the two heme d chiral carbon atoms has been shown to be identical. for both catalases the heme d is rotated 180 degrees about the axis defined by the alpha-gamma-meso carbon atoms, with respect to the orientation found for heme b in beef liver cat ...	1996	8621527
endoglucanase ii (egii) of penicillium janthinellum: cdna sequence, heterologous expression and promotor analysis.	the cdna coding for the endoglucanase egii of p. janthinellum was cloned and sequenced. the open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the t. reesei egl2. the cellulose-binding domain of egii represents a typical member of the a family of cellulases. the egl2 gene is only induced by cellulose or cellobiose and not by sophorose. a promotor fragment including 1 kb was cloned and sequenced. three major transcription startp ...	1996	8625430
primary structures of fungal fructosyl amino acid oxidases and their application to the measurement of glycated proteins.	fructosyl amino acid oxidase (faod), which is active toward model compounds of the glycated proteins in blood, n epsilon-fructosyl n sigma-z-lysine and n-fructosyl valine, was purified to homogeneity from aspergillus terreus gp1. though the enzyme did not use glycated proteins directly as its substrate, it used glycated human serum albumin (hsa) when hsa was treated with a protease. linear relationships between both the concentration and the increase in absorbance and the glycation rate of glyca ...	1996	9022674
the oxidation of pyrene and benzo[a]pyrene by nonbasidiomycete soil fungi.	the purpose of this study was to determine the ability of nonbasidiomycete soil fungi to oxidize pyrene (four rings) and benzo[a]pyrene (bap) (five rings). fungi were isolated from five different soils in which the polycyclic aromatic hydrocarbon content ranged from 0.8 to 80 micrograms/g dry soil. approximately 50% of the isolates in all sites were able to oxidize pyrene. the pyrene-oxidizing species belonged to all fungal divisions except basidiomycetes. the most common were penicillium spp. o ...	1995	7627908
crystal structure of catalase hpii from escherichia coli.	catalase is a ubiquitous enzyme present in both the prokaryotic and eukaryotic cells of aerobic organisms. it serves, in part, to protect the cell from the toxic effects of small peroxides. escherichia coli produces two catalases, hpi and hpii, that are quite distinct from other catalases in physical structure and catalytic properties. hpii, studied in this work, is encoded by the kate gene, and has been characterized as an oligomeric, monofunctional catalase containing one cis-heme d prosthetic ...	1995	7663946
the primary structure of carboxypeptidase s3 from penicillium janthinellum ibt 3991.	the complete amino acid sequence of penicillopeptidase s3, a serine carboxypeptidase isolated from penicillium janthinellum ibt 3991, has been determined. the enzyme consists of 481 amino acids arranged in a single polypeptide chain. six glycosylation sites were established in positions 41, 218, 256, 326, 384 and 392. the molecule contains six cysteinyl residues among which disulfide bridges was established between cys-71-cys-333 and cys-233-cys-289. carboxypeptidase s3 is homologous to carboxyp ...	1995	7664873
distribution and properties of fructosyl amino acid oxidase in fungi.	fructosyl amino acid oxidase, and enzyme that can be used for the determination of glycated proteins in blood samples from diabetic patients, was used to screen cultures in our microorganism culture collection. fructosyl amino acid oxidase was found only in the strains of four genera of fungi, aspergillus, fusarium, gibberella, and penicillium and exhibited different substrate specificities against fructosyl valine and n epsilon-fructosyl n alpha-z-lysine. a fructosyl valine-specific enzyme from ...	1995	8534116
production of extracellular xylanases by penicillium janthinellum. effect of selected growth conditions.	xylanase production by penicillium janthinellum using 10-100 mm of 2,2-dimethylsuccinate (dms) buffer, in a range of ph 4.5-6.0 was studied. the enzyme activity was enhanced using oat xylan as the carbon source. under these conditions a culture produced 1.14 mumol/min (11.4 u/ml or 84.4 u/mg) of beta-xylanase after 5 d of growth in a 10-mm buffer solution at ph 4.5. protease was absent in the dms buffer except when 100 mm phosphate buffer at ph 6.0 was used (4 u/ml). beta-xylosidase was only fou ...	1994	7944349
pea (pisum sativum l.) seed isolectins 1 and 2 and pea root lectin result from carboxypeptidase-like processing of a single gene product.	the complete amino acid sequences of the alpha-subunits of pea (pisum sativum l.) seed and root lectin, the c-terminal amino acids of the beta-subunits of pea seed lectin, and most of the sequence of the beta-subunit of pea root lectin were determined. in contrast to earlier reports it was shown that the beta-subunits of both seed isolectins end at asn-181. the alpha 1 subunits end at gln-241 (major fraction) or lys-240 (minor fraction), whereas the alpha 2 subunits end at ser-239, ser-238, ser- ...	1994	8111028
cloning, sequencing, and heterologous expression of a cellulase-encoding cdna (cbh1) from penicillium janthinellum.	from a penicillium janthinellum cdna library, two clones with 1.8- and 1.9-kb inserts were isolated by hybridization to a trichoderma reesei cellulase-encoding gene probe (egl1). both cdnas have identical 5' ends and coding sequences, but different polyadenylation start points in their 3' untranslated regions. in the nucleotide (nt) sequence, one open reading frame of 537 amino acids was detected which shows 56% homology with endoglucanase i of t. reesei and 70% homology with cellobiohydrolase i ...	1993	8440481
molecular analysis and overexpression of the gene encoding endothiapepsin, an aspartic protease from cryphonectria parasitica.	the gene, epn-1, encoding endothiapepsin (epn), an aspartic protease (aspp) synthesized and secreted by the ascomycete fungus responsible for chestnut blight, cryphonectria (endothia) parasitica, was identified and characterized. inspection of the nucleotide and deduced amino acid (aa) sequences revealed perfect agreement with the experimentally derived 330-aa sequence of mature epn [barkholt, eur. j. biochem. 167 (1987) 327-338] and an additional 89 aa of putative preprosequence. of the nine fu ...	1993	8462868
novel serine penicillocarboxypeptidase cpd-s3 from penicillium janthinellum ibt 3991: purification, characterization, and uses in peptide synthesis and modification.	a novel carboxypeptidase (cpd-s3) from penicillium janthinellum ibt 3991 has been isolated in a two-step purification procedure by cation exchange and affinity chromatography. the enzyme is a serine carboxypeptidase with a denatured molecular mass determined by sds of 62 kda of which 32% is carbohydrate. the isoelectric point is 5.1. cpd-s3 exhibits a high stability towards organic solvents and elevated temperatures. besides the carboxypeptidase activity, cpd-s3 exhibits esterase, amidase, and c ...	1993	7764295
the primary structure of carboxypeptidase s1 from penicillium janthinellum.	the complete amino acid sequence of carboxypeptidase s1 from penicillium janthinellium has been determined by n-terminal sequencing of the reduced and vinylpyridinated protein and of peptides obtained by cleaved with cyanogen bromide, iodosobenzoic acid, hydroxylamine, endoproteinase lysc, endoproteinase aspn and glu-specific proteinase from b. licheniformis. the enzyme consists of a single peptide chain of 433 amino acid residues and contains 9 half-cystine residues and one glycosylated asparag ...	1993	8224168
[identification of toxigenic mould in soft drink causing food poisoning].	in a soft drink caused food poisoning, white floccus was found and mould count was 6.0 x 10(2) cfu/ml. the mycoflora was made of only one kind of mould which was identified as penicillium janthinellum biourge. this isolate can grow under anaerobic condition. the culture liquid of the isolate was fed to mice orally for toxicity test, which made the mice lose weight. an extract of the culture liquid was tested in weaned mice inaberitoneally for toxicity and all mice died in 24h. the toxic symptoms ...	1992	1606874
[dermatomycoses in workers in enterprises of the microbiological industry].	mycologic examination of 54 patients with clinical manifestations of dermatomycosis, engaged in glucose oxidase and catalase production has found a producer fungus, penicillium vitale in 31 (38%); 3 out of 37 people contacting with producers of glucamylase and pectofetidine appeared to be infected by aspergillus awamori (1.1%) and aspergillus foetidus (1.1%), respectively. the producers were isolated from the skin of 40 (16.44%) out of 243 workers having no clinical signs of dermatomycosis, main ...	1992	1427292
laminarinase from penicillium funiculosum and its role in release of beta-glucosidase.	an extracellular laminarinase (1----3)-beta-glucan glucohydrolase (ec 3.2.1.6) was purified from culture filtrates of penicillium funiculosum. it was homogeneous on polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate. it had a mr of 14,000 and isoelectric point of ph 4.2. the apparent km value for lamimarinase was 8.3 mg/ml and vmax was 8 mumol/min/mg. the distribution of beta-glucosidase activity in two different species of penicillium showed that p. funicul ...	1991	1904246
differentiation of species and strains among filamentous fungi by dna fingerprinting.	we have analyzed 11 strains and clones, representing five species (penicillium janthinellum, p. citrioviridae, p. chrysogenum, aspergillus niger, trichoderma harzianum) and three genera of filamentous fungi, for the presence of hypervariable loci in their genomes by hybridization with simple repeat oligonucleotides and the dna of phage m13. the oligonucleotide probes (ct)8, (gtg)5 and (gaca)4, as well as m13 dna, are informative probes for fingerprinting in all genera and species tested. the pro ...	1991	1907892
electrophoretic karyotype of cellulolytic penicillium janthinellum strains.	the genome of the cellulolytic fungus penicillium janthinellum biourge was resolved completely by rotating field electrophoresis. the gel pattern revealed 8-10 different chromosomes. on the basis of data for yeast chromosome size standards from schizosaccharomyces pombe and saccharomyces cerevisiae, chromosome sizes in the range from 2.0 to 8 mb were estimated. by southern hybridization with heterologous probes, the chromosomal locations of rdna, the elongation factor ef1, actin and ubiquitin ha ...	1991	1934133
comparative spectral analysis of mammalian, fungal, and bacterial catalases. resonance raman evidence for iron-tyrosinate coordination.	resonance raman spectra are reported for catalases from bovine liver, the ascomycete fungus aspergillus niger, and the bacterium micrococcus luteus. the vibrational frequencies of the oxidation-, spin-, and coordination number-sensitive spectral bands are indicative of high spin pentacoordinate hemes in the resting ferric enzymes of each of these organisms. this result is in accord with the crystal structure of bovine catalase (fita, i., and rossmann, m.g. (1985) j. mol. biol. 185, 21-37). in co ...	1989	2753885
carboxypeptidase s-1 from penicillium janthinellum: enzymatic properties in hydrolysis and aminolysis reactions.	carboxypeptidase s-1 from penicillium janthinellum has been isolated by affinity chromatography and characterized. the enzyme activity is unusually stable in organic solvents, e.g. 80% methanol. the hydrolysis of peptide substrates is apparently dependent on three ionizable groups. one group, with pka of 4.0-4.5, is a catalytically essential residue in its deprotonated form, and another group with a pka of 6.5-7.0 functions in its protonated form, apparently as the binding site for the c-termina ...	1988	3256309
amino acid sequence of endothiapepsin. complete primary structure of the aspartic protease from endothia parasitica.	the amino acid sequence of endothiapepsin, the aspartic protease from endothia parasitica has been determined. the enzyme consists of 330 residues. the sequence determination was performed exclusively at the protein level. the homology of this fungal milk-clotting enzyme with aspartic proteases is demonstrated by alignment with pepsin, chymosin, gastricsin, renin, and cathepsin d from various vertebrates and proteinase a from saccharomyces cerevisiae showing 25-30% identity. the identity with mu ...	1987	3305016
nucleotide sequence of the saccharomyces cerevisiae ctt1 gene and deduced amino-acid sequence of yeast catalase t.	a 2642-base-pair dna fragment containing the catalase t (ctt1) structural gene of the yeast saccharomyces cerevisiae and its flanking regions has been sequenced. the gene codes for a protein of 562 amino acids (relative molecular mass 64,449) and appears to contain no intron. the amino acid sequence of catalase t derived from the dna sequence shows 40.7% homology (52.2% including conservative replacements) to that of bovine liver catalase. all amino acids previously postulated to participate dir ...	1986	3536508
comparison of beef liver and penicillium vitale catalases.	the structures of penicillium vitale and beef liver catalase have been determined to atomic resolution. both catalases are tetrameric proteins with deeply buried heme groups. the amino acid sequence of beef liver catalase is known and contains (at least) 506 amino acid residues. although the sequence of p. vitale catalase has not yet been determined chemically, 670 residues have been built into the 2 a resolution electron density map and have been given tentative assignments. a large portion of ...	1986	3712444
three-dimensional structure of catalase from penicillium vitale at 2.0 a resolution.	the three-dimensional structure analysis of crystalline fungal catalase from penicillium vitale has been extended to 2.0 a resolution. the crystals belong to space group p3(1)21, with the unit cell parameters of a = b = 144.4 a and c = 133.8 a. the asymmetric unit contains half a tetrameric molecule of 222 symmetry. each subunit is a single polypeptide chain of approximately 670 amino acid residues and binds one heme group. the amino acid sequence has been tentatively determined by computer grap ...	1986	3712443
release of cellulases from penicillium janthinellum.		1986	18553855
penicillopepsin, the aspartic proteinase from penicillium janthinellum: substrate-binding effects and intermediates in transpeptidation reactions.		1985	3912235
the nadph binding site on beef liver catalase.	beef liver and human erythrocyte catalases (ec 1.11.1.6) bind nadp tenaciously [kirkman, h. n. & gaetani, g. f. (1984) proc. natl. acad. sci. usa 81, 4343-4348]. the position of nadp on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. in contrast to nearly all other known structures of protein-bound nadp, nad, and fad, the nadp molecule of beef l ...	1985	3856839
autoradiographic method to screen for soil microorganisms which accumulate zinc.	an autoradiographic method was developed to screen for and isolate soil microorganisms which accumulate zinc (zn). diluted soil samples (rubicon fine sand, entic haplorthods [ph 5.9]) were plated on soil extract-glucose agar containing radioactive 65zn. after 7 days of incubation, individual colonies which accumulated sufficient 65zn could be detected by autoradiography. these colonies were isolated and confirmed as zn accumulators in pure culture by using the autoradiographic plate technique. m ...	1985	3883897
action of serine carboxypeptidases on endopeptidase substrates, peptide-4-methyl-coumaryl-7-amides.	carboxypeptidase y hydrolyzed n-substituted peptide-4-methylcoumarin-7-amides (peptide-nh-mec) at ph 7 by releasing 7-amino-4-methylcoumarin (nh2-mec) which was then followed by carboxypeptidase action. in particular, a chymotrypsin-directed substrate, suc-leu-leu-val-tyr-nh-mec, was hydrolyzed by the enzyme with a second-order rate constant of 7200 m-1 s-1, which is compatible with the rate for an anilide substrate and some n-substituted dipeptides. the activity was completely inhibited by phen ...	1985	3905405
the active center of catalase.	the refined structure of beef liver catalase (i. fita, a. m. silva, m. r. n. murthy & m. g. rossmann, unpublished results) is here examined with regard to possible catalytic mechanisms. the distal side of the deeply buried heme pocket is connected with the surface of the molecule by one (or possibly two) channel. the electron density representing the heme group, in each of the two crystallographically independent subunits, is consistent with degradation of the porphyrin rings. the heme group app ...	1985	4046038
repression of endo-1,4-beta-glucanase formation in penicillium janthinellum and product inhibition of its 1,4-beta-glucanases and cellobiases.	endo-1,4-beta-glucanase formation of penicillium janthinellum was repressed by glucose, sophorose, and glycerol. chromatography on deae-sephadex a-50 was employed to separate the 1,4-beta-glucanases from two cellobiases. the 1,4-beta-glucanases were inhibited competitively by cellobiose and glucose, and the two cellobiases were inhibited by glucose and glucono-delta-lactone.	1982	6799497
formation and location of 1,4-beta-glucanases and 1,4-beta-glucosidases from penicillium janthinellum.	formation and location of 1,4-beta-glucanases and 1,4-beta-glucosidases were studied in cultures of penicillium janthinellum grown on avicel, sodium carboxymethyl cellulose, cellobiose, glucose, mannose, and maltose. endo-1,4-beta-glucanases were found to be cell free, and their formation was induced by cellobiose. 1,4-beta-glucosidases, on the other hand, were formed constitutively and were primarily cell free, but with a small amount strongly associated with the cell wall. low 1,4-beta-glucosi ...	1981	16345751
[comparative kinetics of reactions catalyzed by glucose oxidase in the presence of different electron acceptors].	it was shown that six redox indicators, beside oxygen, can be used as substrates for glucose oxidase from penicillium vitale. the ph dependence of the rate of glucose oxidase-catalyzed reactions in the presence of oxygen and artificial electron acceptors was studied. in contrast to the reaction involving oxygen, in which the maxima of the ph activity profile were observed at ph 5.6, glucose oxidase reveals maximal ph activity profile at ph 7.5 -- 7.6 in the presence of phenasine methosulfate, ba ...	1981	7284485
growth of penicillium janthinellum on glycine as sole carbon and nitrogen source.	penicillium janthinellum is able to grow on glycine as the sole carbon and nitrogen source. the amino acid is transaminated to glyoxylate which is further metabolised to pyruvate by the glycerate pathway. the reaction product of partially purified glycerate kinase from this fungus is 2-phosphoglycerate. phosphoglycerate mutase initiates gluconeogenesis from glycine. partially purified phosphoglycerate mutase is inhibited by fructose 6-phosphate. the possible significance of this regulation is di ...	1980	6251917
the janthitrems: fluorescent tremorgenic toxins produced by penicillium janthinellum isolates from ryegrass pastures.	new tremorgenic mycotoxins named janthitrem a, b, and c (molecular weights 601, 585, and 569, respectively) were produced by more than half of 21 penicillium janthinellum isolates obtained from ryegrass pastures involved in ryegrass staggers outbreaks in sheep.	1980	7356319
the primary structure of calf chymosin.	the complete amino acid sequence of calf chymosin (rennin) (ec 3.4.23.4) has been determined. the sequence consists of a single peptide chain of 323 amino acid residues. the primary structure of the precursor part of calf prochymosin was published previously (pedersen, v.b., and foltmann, b. (1975) eur. j. biochem. 55, 95-103), thus we are now able to account for the total 365 amino acid residues of calf prochymosin. comparison of the sequence of calf prochymosin with that of pig pepsinogen a (e ...	1979	381305
production of tremorgenic toxins by penicillium janthinellum biourge: a possible aetiological factor in ryegrass staggers.	topsoil, herbage and faeces collected during an outbreak of ryegrass staggers in sheep were examined for tremorgenic penicillia. no such fungi were recovered from the plant material, but they were found among the predominant fungi in the soil and faecal samples. the commonest species of penicillium, and almost the only tremorgenic species encountered, was penicillium janthinellum biourge. when fed to sheep, the mycelium of this fungus evoked a number of the clinical signs seen in field cases of ...	1979	475667
the effects of temperature on growth of four high arctic soil fungi in a three-phase system.	the effect of temperature on the growth of chrysosporium pannorum, cylindrocarpon sp., penicillium janthinellum, and phoma herbarum, isolated from tundra soils, was studied. the growth in two systems, glucose-mineral agar plates and sand, moistened with glucose-mineral broth, was compared. all isolates showed an exponential increase in mass (measured as protein increase) in sand and a linear rate of extension on agar. radial increase on agar was shown not to be a good index of growth in sand. tr ...	1978	565246
[effect of amino acids on transformation of glucose oxidase as antigen in tissues of immunized animals].	the effect of certain amino acids on transformation of glucoseoxidase as an antigen in different tissues of the animals immunized with it showed that the used glucoseoxidase of the fungus penicillium vitale pidopl. et bilaj possesses antigenic properties peculiar to this enzyme isolated from other sources. 1.5 minutes after a single administration of the antigen to nonimmunized rabbits it is determined in them in descending amounts in such an order: blood, lungs, liver, kidneys, lymphatic nodes. ...	1978	567396
[spectral properties of the prosthetic group of penicillium vitale catalase].	differences in the absorption spectrum of the penicillum vitale catalase in the visible region as compared to the absorption spectrum for catalase of animal origin are established to be due to the prosthetic group of the enzyme. a molecule of p. vitale catalase is determined to contain 0.051 +/- 0.0003% of iron. it corresponds to two iron atoms per enzyme molecule and to a twice as low content of iron as in a molecule of the bovine liver catalase. an assumption is advanced that the p. vitale cat ...	1978	664035
kinetics and stability of glucooxidase from penicillium vitale.	the kinetics, thermal stability and ultrasonic resistance of glucosoxidase from penicillium vitale have been investigated. its oxidative constant is close to, catalytic constant is 1.7 times less and the reductive constant is 5.4 times more than the respective constants of the enzyme from aspergillus niger. the relationship between the thermal inactivation constant (k(i)=2.1 pt 15.3. the same relationship is true of the ultrasonic inactivation constant and ph in the ph range of 6.5-8.0. in a mor ...	1978	27781
purification & properties of an extracellular dextranase from penicillium janthinellum.		1978	748164
[stabilization of modified glucose oxidase from penicillium vitale incorporated into the polymeric chains of gels].	glucose oxidase from penicillium vitale was modified by unsaturated compounds, e.g. acrolein, allylisothiocyanate, acryloyl chloride and maleic anhydride. the degree of modification for the respective agents made up to 27.2, 8.3, 11.1 and 35.0% of the amine residues; the enzymatic activity was thereby retained by 98, 100, 0.03 and 58%, respectively. the thermal stability of modified enzymes was decreased 2-7 times. the modified preparations were copolymerized with acrylamide or 2-hydroxyethyl me ...	1978	656485
penicillopepsin from penicillium janthinellum crystal structure at 2.8 a and sequence homology with porcine pepsin.	the polypeptide chain of the acid protease penicillo pepsin folds via an 18-stranded mixed beta-sheet into two distinct lobes separated by a 30-a long groove which is the extended substrate binding site. the catalytic residues asp-32 and asp-215 are located in this groove and their carboxyl groups are in intimate contact. alignment of the amino acid sequence with that of pepsin shows regions of high homology.	1977	323722
penicillopepsin: 2.8 a structure, active site conformation and mechanistic implications.	the crystal structure of penicillopepsin, an extracellular acid protease isolated from the mold penicillium janthinellum, has been determined at 2.8 a resolution by the method of multiple isomorphous replacement. the resulting electron density map computed from the native structure factor amplitudes and mir phases has an overall mean figure of merit of 0.90. the molecule is decidedly nonspherical, with the majority of residues in beta-structure. there is an 18-stranded mixed beta-sheet which for ...	1977	339694
microbial degradation of the thiolcarbamate herbicide, diallate, in soils and by pure cultures of soil microorganisms.	the disappearance of the herbicide, avadex (40% diallate), from five agricultural soils (differing in either ph, carbon content, or nitrogen content), incubated under sterile and non-sterile conditions, was followed for a period of 20 weeks. avadex was rapidly lost from microbiologically active soils, with over 50% of the applied (2.5 ppm) dosage disappearing within four weeks; losses from sterile soils were much slower with recoveries of over 50% after 20 weeks. incubation of soil with avadex t ...	1976	1267481
[method of purification of glucose oxidase by means of affinity chromatography on immunoabsorbent].	the possibility to purify glucose oxidase from penicillium vitale on immunosorbent containing specific antibodies to the enzyme covalently bound with sepharose 4b is studied. the method of affinity chromatography was applied, beside routine methods of fractionating blood serum proteins, to isolate specific antibodies from antiserum of rabbits immunized with glucose oxidase. immobilized on sepharose glucose oxidase was used as biospecific sorbent. specific antibodies to the enzyme were isolated u ...	1975	1203397
kininase and anti-inflammatory activities of acid carboxypeptidase from penicillium janthinellum.	the acid carboxypeptidase from penicillium janthinellum catalyzed the rapid release of arginine, and the slow release of phenylalanine, proline, serine and glycine from the carboxy-terminal of bradykinin at ph 4.15 to 4.8. anti-inflammatory activity of the acid carboxypeptidase seems to suggest that the enzyme hydrolyzed bradykinin in vivo.	1975	1204716
action of crystalline acid carboxypeptidase from penicillium janthinellum.	acid carboxypeptidase (ec 3.4.12.-) crystallized from culture filtrate of penicillium janthinellum has been investigated for its use in carboxy-terminal sequence determination of z-gly-pro-leu-gly, z-gly-pro-leu-gly-pro, angiotensin i, native lysozyme, native ribonuclease t1, and reduced s-carboxy-methyl-lysozyme. the examination indicated that proline and glycine were liberated from z-gly-pro-leu-gly-pro. at high enzyme concentration, the enzyme catalyzed complete sequential release of amino ac ...	1975	239751
[preparation in crystalline form of catalase from penicillium vitale pidopl. et bilai].		1975	1204470
[variability in penicillium janthinellum biourge, a producer of the antibiotic janthinellin, under the action of nitrosomethylurea].	variation of the janthinellin-producing organism p. janthinellum was induced by nitrozomethylurea (nmu). the following concentrations of nmu were tested: 0.5; 0.25, 0.125 per cent at the exposure time of 15 minutes, 1 and 10 hours. the conidia survival had back dependence on the concentration and exposure time. the morphological variation was evident from the presence of forms with changed colour of the colony surface mycelium, light green, brown, light yellow, yellow-pink and white conidia and ...	1975	1211902
purification and properties of an extracellular acid ribonuclease from penicillium janthinellum.		1974	4371002
submerged production, purification, and crystallization of acid carboxypeptidase from penicillium janthinellum ifo-8070.	penicillium janthinellum ifo-8070 produced an acid carboxypeptidase of molecular weight 51,000 in a liquid medium at 25 c. maximum enzyme concentration was obtained within 3 to 6 days in a medium containing 2% wheat bran, 1% defatted soybean, and 1% kh(2)po(4); the initial ph was 2 to 4. when submerged aerobic conditions were used, a 51,000-molecular-weight acid carboxypeptidase was produced and no detectable amounts of 160,000-molecular-weight acid carboxypeptidase were produced. acid carboxype ...	1974	4474829
[study of tubular crystals of penicillium vitale glucose oxidase and its quaternary structure].		1973	4754779
influence of inorganic nitrate on the formation of extracellular protease and ribonuclease by penicillium janthinellum.		1973	4762798
[formation of glucose oxidase and catalase during the growth of penicillium vitale pidopl. et bilai on media with different nitrogen and carbon ratios].		1972	4667024
[obtaining purified preparations of catalase from the fungus penicillium vitale pidopl. et bilai].		1972	4661073
large-scale preparation and some properties of penicillopepsin, the acid proteinase of penicillium janthinellum.		1970	5418964
a crystalline proteinase (peptidase a) from penicillium janthinellum: preliminary x-ray data.		1969	5346053
[natural variability of the strain penicillium janthinellum biourge--producer of janthinellin].		1968	5707360
a pepsin-like enzyme from penicillium janthinellum.		1968	4866867
the role of a protease in sporulation of penicillium janthinellum.		1966	5961655
[on some properties of crystalline and purified non-crystalline glucose-oxidase preparations from penicillium vitale pidopl. et bilai].		1965	5869922
proteolytic enzymes of penicillium janthinellum. ii. properties of peptidase b.		1964	14264888
proteolytic enzymes of penicillium janthinellum. i. purification and properties of a trypsinogen-activating enzyme (peptidase a).		1964	14264887
[isolation of glucose oxidase from penicillium vitale pidopl. et bilai].		1964	14335573
effect of salinity and temperature on coccidioides immitis and three antagonistic soil saprophytes.	egeberg, roger o. (university of southern california, los angeles), ann f. elconin, and margaret c. egeberg. effect of salinity and temperature on coccidioides immitis and three antagonistic soil saprophytes. j. bacteriol. 88:473-476. 1964.-experiments exploring the factors which determine the ability of coccidioides immitis to survive and reproduce in the soil in situ are described and discussed. previous work indicated that soil temperatures and salinity influence the growth and distribution o ...	1964	14203366
[iantinellin--an antibiotic with antifungal properties produced from penicillium janthinellum biourge].		1963	13970363
[an industrial method for the purification and crystallization of and some properties of glucose oxidase from the fungus penicillium vitale pidopl. et bilai].		1962	13902922