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transgalactosylation of lactose for synthesis of galacto-oligosaccharides using kluyveromyces marxianus ncim 3551.among a number of yeast strains screened for whole cell transgalactosylating activity, kluyveromyces marxianus ncim 3551 was found to be most suitable biocatalyst for production of galacto-oligosaccharides (gos). cell permeabilization lead to an efficient bioconversion by β-galactosidase resulting in synthesis of gos. a maximum gos yield of 36% (w/w) of total sugars was achieved and the products consisted of tri- and tetra-galacto-oligosaccharides. a lactose conversion rate of 80% and productivi ...201525976627
[expression of inulinase genes in the yeasts saccharomyces cerevisiae and kluyveromyces marxianus].expression of the genes encoding the enzymes involved in inulin, sucrose, and glucose metabolism in the yeasts saccharomyces cerevisiae and kluyveromyces marxianus was studied. the exon-intron structure of the relevant genes was identified and the primers for quantitative pcr were optimized. expression of the genes was found to depend on the carbon source. glucose was shown to exhibit a repressive effect on inulinase synthesis by k. marxianus, while in s. cerevisiae glucose and sucrose were inul ...201525916145
complete genome sequence of kluyveromyces marxianus nbrc1777, a nonconventional thermotolerant yeast.we determined the genome sequence of the thermotolerant yeast kluyveromyces marxianus strain nbrc1777. the genome of strain nbrc1777 is composed of 4,912 open reading frames (orfs) on 8 chromosomes, with a total size of 10,895,581 bp, including mitochondrial dna.201525908152
synthetic signal sequences that enable efficient secretory protein production in the yeast kluyveromyces marxianus.targeting of cellular proteins to the extracellular environment is directed by a secretory signal sequence located at the n-terminus of a secretory protein. these signal sequences usually contain an n-terminal basic amino acid followed by a stretch containing hydrophobic residues, although no consensus signal sequence has been identified. in this study, simple modeling of signal sequences was attempted using gaussia princeps secretory luciferase (gluc) in the yeast kluyveromyces marxianus, which ...201525889890
genetic basis of the highly efficient yeast kluyveromyces marxianus: complete genome sequence and transcriptome analyses.high-temperature fermentation technology with thermotolerant microbes has been expected to reduce the cost of bioconversion of cellulosic biomass to fuels or chemicals. thermotolerant kluyveromyces marxianus possesses intrinsic abilities to ferment and assimilate a wide variety of substrates including xylose and to efficiently produce proteins. these capabilities have been found to exceed those of the traditional ethanol producer saccharomyces cerevisiae or lignocellulose-bioconvertible ethanolo ...201525834639
comparison of a pectinolytic extract of kluyveromyces marxianus and a commercial enzyme preparation in the production of ives (vitis labrusca) grape juice.this study analyses the effect of the crude enzymatic extract produced by kluyveromyces marxianus (eeb) in the maceration and clarification of juice produced from ives (vitis labrusca) grapes compared to the commercial enzyme preparation pectinex(®)ultra color (pec). treatments were conducted with a total pectinolytic activity of 1 u/ml of fruit juice, at 40 °c, for 60 min. after the enzymatic treatment, the juices were evaluated with respect to yield, viscosity, and degree of clarification, as ...201525721421
[investigation of mechanisms of interaction between inulinase from kluyveromyces marxianus and the matrices of ion-exchange resins and fiber].it is established that ion exchange resins av-17-2p, ku-2, av-16-gs, am 21a, imac-hp, purolite and fiber vion kn-1 can be applied as carriers for inulinase immobilization. the analysis of ir spectra for an enzyme, carriers and heterogeneous enzyme preparations showed that inulinase binding to matrices of various carriers occurs in general through electrostatic interactions. it is assumed that the mechanisms of interaction between inulinase from kluyveromyces marxianus and the matrices of cation- ...201525702479
enhanced enzymatic hydrolysis and ethanol production from cashew apple bagasse pretreated with alkaline hydrogen peroxide.the effect of combinations and ratios between different enzymes has been investigated in order to assess the optimal conditions for hydrolysis of cashew apple bagasse pretreated with alkaline hydrogen peroxide (the solids named cab-ahp). the separate hydrolysis and fermentation (shf) and simultaneous saccharification and fermentation (ssf) processes were evaluated in the ethanol production. the enzymatic hydrolysis conducted with cellulase complex and β-glucosidase in a ratio of 0.61:0.39, enzym ...201525545094
studies on the mechanism of synthesis of ethyl acetate in kluyveromyces marxianus dsm 5422.kluyveromyces marxianus converts whey-borne sugar into ethyl acetate, an environmentally friendly solvent with many applications. k. marxianus dsm 5422 presumably synthesizes ethyl acetate from acetyl-scoa. iron limitation as a trigger for this synthesis is explained by a diminished aconitase and succinate dehydrogenase activity (both enzymes depend on iron) causing diversion of acetyl-scoa from the tricarboxic acid cycle to ester synthesis. copper limitation as another trigger for ester synthes ...201525487884
isotope labeling pattern study of central carbon metabolites using gc/ms.determination of fluxes by (13)c tracer experiments depends on monitoring the (13)c labeling pattern of metabolites during isotope experiments. in metabolome-based (13)c metabolic flux analysis, liquid chromatography combined with mass spectrometry or tandem mass spectrometry (lc/ms or lc/ms/ms, respectively) has been mainly used as an analytical platform for isotope pattern studies of central carbon metabolites. however, gas chromatography with mass spectrometry (gc/ms) has several advantages o ...201525463204
cell factory applications of the yeast kluyveromyces marxianus for the biotechnological production of natural flavour and fragrance molecules.kluyveromyces marxianus is emerging as a new platform organism for the production of flavour and fragrance (f&f) compounds. this food-grade yeast has advantageous traits, such as thermotolerance and rapid growth, that make it attractive for cell factory applications. the major impediment to its development has been limited fundamental knowledge of its genetics and physiology, but this is rapidly changing. k. marxianus produces a wide array of volatile molecules and contributes to the flavour of ...201525393382
screening of accurate clones for gene synthesis in yeast.methods for error-less gene synthesis are desired because synthesized genes often contain mutations. by cloning pcr-assembled oligonucleotide fragments fused to a selection marker in yeast, we developed a novel method to screen accurate clones in gene synthesis. as a model case, the 555-bp luciferase gene from gaussia princeps (gluc) was synthesized to contain yeast-optimized codons (called ygluc hereafter). after standard pcr-mediated oligonucleotide assembly, many clones showed no luciferase a ...201525201012
data for rapid ethanol production at elevated temperatures by engineered thermotolerant kluyveromyces marxianus via the nadp(h)-preferring xylose reductase-xylitol dehydrogenase pathway.a thermo-tolerant nadp(h)-preferring xylose pathway was constructed in kluyveromyces marxianus for ethanol production with xylose at elevated temperatures (zhang et al., 2015 [25]). ethanol production yield and efficiency was enhanced by pathway engineering in the engineered strains. the constructed strain, yzj088, has the ability to co-ferment glucose and xylose for ethanol and xylitol production, which is a critical step toward enabling economic biofuel production from lignocellulosic biomass. ...201526543879
protein enrichment of an opuntia ficus-indica cladode hydrolysate by cultivation of candida utilis and kluyveromyces marxianus.the cladodes of opuntia ficus-indica (prickly pear cactus) have a low protein content; for use as a balanced feed, supplementation with other protein sources is therefore desirable. we investigated protein enrichment by cultivation of the yeasts candida utilis and kluyveromyces marxianus in an enzymatic hydrolysate of the cladode biomass.201525371280
influence of carbon and nitrogen source on production of volatile fragrance and flavour metabolites by the yeast kluyveromyces marxianus.the yeast kluyveromyces marxianus produces a range of volatile molecules with applications as fragrances or flavours. the purpose of this study was to establish how nutritional conditions influence the production of these metabolites. four strains were grown on synthetic media, using a variety of carbon and nitrogen sources and volatile metabolites analysed using gas chromatography-mass spectrometry (gc-ms). the nitrogen source had pronounced effects on metabolite production: levels of the fusel ...201525308412
growth and by-product profiles of kluyveromyces marxianus cells immobilized in foamed alginate.the aim of this research was to study how the yeast cell immobilization technique influences the growth and fermentation profiles of kluyveromyces marxianus cultivated on apple/chokeberry and apple/cranberry pomaces. encapsulation of the cells was performed by droplet formation from a foamed alginate solution. the growth and metabolic profiles were evaluated for both free and immobilized cells. culture media with fruit waste produced good growth of free as well as immobilized yeast cells. the fe ...201525277269
whey fermentation by yeast strains kluyveromyces marxianus ucm y-2096 and ucm y-2388.two kluyveromyces marxianus strains have been tested for their ability to ferment lactose in the medium containing cheese whey powder (cwp) and lactose at different concentrations. the ethanol production by the selected strains was inhibited by the increase in substrate concentration (100 g/l). the strain k. marxianus ucm y-2096 was found to yield the higher ethanol formation and to be more resistant to ethanol under microaerophilic conditions.201524800512
optimizing promoters and secretory signal sequences for producing ethanol from inulin by recombinant saccharomyces cerevisiae carrying kluyveromyces marxianus inulinase.inulin is a polyfructan that is abundant in plants such as jerusalem artichoke, chicory and dahlia. inulinase can easily hydrolyze inulin to fructose, which is consumed by microorganisms. generally, saccharomyces cerevisiae, an industrial workhorse strain for bioethanol production, is known for not having inulinase activity. the inulinase gene from kluyveromyces marxianus (kminu), with the ability of converting inulin to fructose, was introduced into s. cerevisiae d452-2. the inulinase gene was ...201525142154
yeasts from kefir grains: isolation, identification, and probiotic characterization.kefir-a traditional beverage whose consumption has been associated with health benefits-is a logical natural product to investigate for new probiotic strains. the aim of the present work was to isolate and identify kefir yeasts and select those with acid and bile tolerance to study their adhesion to epithelial cells and their transit through mouse gut. from 4 milky and 3 sugary kefir grains, 34 yeast strains were isolated and identified by means of classical microbiological and molecular-genetic ...201423824665
physiological characterization of thermotolerant yeast for cellulosic ethanol production.the conversion of lignocellulose into fermentable sugars is considered a promising alternative for increasing ethanol production. higher fermentation yield has been achieved through the process of simultaneous saccharification and fermentation (ssf). in this study, a comparison was performed between the yeast species saccharomyces cerevisiae and kluyveromyces marxianus for their potential use in ssf process. three strains of s. cerevisiae were evaluated: two are widely used in the brazilian etha ...201424535257
hydrolysis of agave fourcroydes lemaire (henequen) leaf juice and fermentation with kluyveromyces marxianus for ethanol production.carbon sources for biofuel production are wide-ranging and their availability depends on the climate and soil conditions of the land where the production chain is located. henequen (agave fourcroydes lem.) is cultivated in yucatán, mexico to produce natural fibers from the leaves, and a juice containing fructans is produced during this process. fructans can be hydrolyzed to fructose and glucose and metabolized into ethanol by appropriate yeasts. in mexico, different agave species provide the car ...201424529165
a broad-range yeast expression system reveals arxula adeninivorans expressing a fungal self-sufficient cytochrome p450 monooxygenase as an excellent whole-cell biocatalyst.the feasibility of using a single vector to clone a cytochrome p450 monooxygenase (p450) in different yeasts and then compare whole-cell hydroxylase activity was investigated. a broad-range yeast expression vector using the yltefp to drive expression of the cloned gene and the sctefp to drive the hygromycin resistance marker gene was used to clone the genes encoding two self-sufficient p450s, cyp102a1 and cyp505a1. both genes were cloned into saccharomyces cerevisiae, kluyveromyces marxianus, ya ...201424521080
permeabilization of kluyveromyces marxianus with mild detergent for whey lactose hydrolysis and augmentation of mixed culture.cheese whey is a by-product of cheese-manufacturing industries, and the utilization of whey is a challenging problem either to use it or dispose it, because only few microorganisms can metabolize the whey lactose. enzymatic hydrolysis of whey lactose to glucose and galactose by β-galactosidase is the approach for biotechnological application. kluyveromyces marxianus cells were permeabilized with non-toxic, biodegradable, anionic detergent n-lauroyl sarcosine (n-ls) for the enzyme activity. the p ...201424500798
novel antihypertensive lactoferrin-derived peptides produced by kluyveromyces marxianus: gastrointestinal stability profile and in vivo angiotensin i-converting enzyme (ace) inhibition.novel antihypertensive peptides released by kluyveromyces marxianus from bovine lactoferrin (lf) have been identified. k. marxianus lf permeate was fractionated by semipreparative high performance liquid chromatography and 35 peptides contained in the angiotensin i-converting enzyme (ace)-inhibitory fractions were identified by using an ion trap mass spectrometer. on the basis of peptide abundance and common structural features, six peptides were chemically synthesized. four of them (dpyklrp, py ...201424476136
effects of signal sequences and folding accessory proteins on extracellular expression of carboxypeptidase y in recombinant saccharomyces cerevisiae.carboxypeptidase y (cpy) is a yeast vacuolar protease with useful applications including c-terminal sequencing of peptides and terminal modification of target proteins. to overexpress cpy with the pro-sequence (procpy) encoded by the saccharomyces cerevisiae prc1 gene in recombinant s. cerevisiae, the procpy gene was combined with the gene coding for a signal sequence of s. cerevisiae mating factor α (mfα), invertase (suc2), or kluyveromyces marxianus inulinase (inu1). among the three constructs ...201424146283
solid state fermentation of food waste mixtures for single cell protein, aroma volatiles and fat production.growth of selected microorganisms of industrial interest (saccharomyces cerevisiae, kluyveromyces marxianus and kefir) by solid state fermentation (ssf) of various food industry waste mixtures was studied. the fermented products were analysed for protein, and nutrient minerals content, as well as for aroma volatile compounds by gc/ms. the substrate fermented by k. marxianus contained the highest sum of fat and protein concentration (59.2% w/w dm) and therefore it could be considered for utilisat ...201424128535
albariño wine aroma enhancement through the use of a recombinant polygalacturonase from kluyveromyces marxianus.the possible biotechnological application of a recombinant endopolygalacturonase of kluyveromyces marxianus (kmpg) for the aroma enhancement of albariño wine was studied. the addition of this enzyme to the must gives rise to a significant increase of the total compounds responsible for the aroma as opposed to the effect when using a commercial pectic enzyme. this increase also results in a significant rise of the odoriferous aglycones which are direct determinants of the aroma. wines made by usi ...201424128465
ethanol production from sunflower meal biomass by simultaneous saccharification and fermentation (ssf) with kluyveromyces marxianus atcc 36907.the lignocellulosic materials are considered promising renewable resources for ethanol production, but improvements in the processes should be studied to reduce operating costs. thus, the appropriate enzyme loading for cellulose saccharification is critical for process economics. this study aimed at evaluating the concentration of cellulase and β-glucosidase in the production of bioethanol by simultaneous saccharification and fermentation (ssf) of sunflower meal biomass. the sunflower biomass wa ...201424794173
perspectives for the biotechnological production of ethyl acetate by yeasts.ethyl acetate is an environmentally friendly solvent with many industrial applications. the production of ethyl acetate currently proceeds by energy-intensive petrochemical processes which are based on natural gas and crude oil without exception. microbial synthesis of ethyl acetate could become an interesting alternative. the formation of esters as aroma compounds in food has been repeatedly reviewed, but a survey which deals with microbial synthesis of ethyl acetate as a bulk product is missin ...201424788328
development of a hybrid fermentation-enzymatic bioprocess for the production of ethyl lactate from dairy waste.this work explores the potential for the development of a hybrid fermentation-enzymatic process for the production of ethyl lactate from dairy waste. cheese whey was used in kluyveromyces marxianus and lactobacillus bulgaricus batch cultures to produce ethanol and lactic acid respectively. subsequently, the fermentation products were transferred into an organic phase through liquid-liquid extraction and ethyl lactate was formed in an esterification reaction catalyzed by lipases. the production o ...201424785788
growth inhibition of thermotolerant yeast, kluyveromyces marxianus, in hydrolysates from cassava pulp.in this study, we report the inhibition of kluyveromyces marxianus tistr5925 growth and ethanol fermentation in the presence of furan derivatives and weak acids (acetic acid and lactic acid) at high temperatures. cassava pulp, obtained as the waste from starch processing, was collected from 14 starch factories located in several provinces of thailand. at a high temperature (42 °c), the cassava pulp hydrolysate from some starch factories strongly inhibited growth and ethanol production of both k. ...201424781978
a biosynthetic pathway for hexanoic acid production in kluyveromyces marxianus.hexanoic acid can be used for diverse industrial applications and is a precursor for fine chemistry. although some natural microorganisms have been screened and evolved to produce hexanoic acid, the construction of an engineered biosynthetic pathway for producing hexanoic acid in yeast has not been reported. here we constructed hexanoic acid pathways in kluyveromyces marxianus by integrating 5 combinations of seven genes (atob, bktb, crt, hbd, mct1, ter, and tes1), by which random chromosomal si ...201424768798
effect of oxygenation and temperature on glucose-xylose fermentation in kluyveromyces marxianus cbs712 strain.the yeast kluyveromyces marxianus features specific traits that render it attractive for industrial applications. these include production of ethanol which, together with thermotolerance and the ability to grow with a high specific growth rate on a wide range of substrates, could make it an alternative to saccharomyces cerevisiae as an ethanol producer. however, its ability to co-ferment c5 and c6 sugars under oxygen-limited conditions is far from being fully characterized.201424712908
industrial robust yeast isolates with great potential for fermentation of lignocellulosic biomass.the search of robust microorganisms is essential to design sustainable processes of second generation bioethanol. yeast strains isolated from industrial environments are generally recognised to present an increased stress tolerance but no specific information is available on their tolerance towards inhibitors that come from the pretreatment of lignocellulosic materials. in this work, a strategy for the selection of different yeasts using hydrothermal hydrolysate from eucalyptus globulus wood, co ...201424704884
interactions between kluyveromyces marxianus and saccharomyces cerevisiae in tequila must type medium fermentation.traditional tequila fermentation is a complex microbial process performed by different indigenous yeast species. usually, they are classified in two families: saccharomyces and non-saccharomyces species. using mixed starter cultures of several yeasts genera and species is nowadays considered to be beneficial to enhance the sensorial characteristics of the final products (taste, odor). however, microbial interactions occurring in such fermentations need to be better understood to improve the proc ...201424677041
potential of some yeast strains in the stereoselective synthesis of (r)-(-)-phenylacetylcarbinol and (s)-(+)-phenylacetylcarbinol and their reduced 1,2-dialcohol derivatives.whole cells of different yeast species have been widely used for a number of asymmetric transformations. in the present study, the screening of several yeast strains revealed the utility of debaryomyces etchellsii in acyloin condensation for (r)-(-)-phenylacetylcarbinol production. some conditions for the efficient biotransformation of benzaldehyde and minimization in the production of by-products were explored: ph of the reaction medium, use of additives (ethanol or acetonitrile), temperature, ...201424671162
modeling the microbial growth and temperature profile in a fixed-bed bioreactor.aiming to scale up and apply control and optimization strategies, currently is required the development of accurate plant models to forecast the process nonlinear dynamics. in this work, a mathematical model to predict the growth of the kluyveromyces marxianus and temperature profile in a fixed-bed bioreactor for solid-state fermentation using sugarcane bagasse as substrate was built up. a parameter estimation technique was performed to fit the mathematical model to the experimental data. the es ...201424658796
production and characterization of β-glucanase secreted by the yeast kluyveromyces marxianus.an extracellular β-glucanase secreted by kluyveromyces marxianus was identified for the first time. the optimal conditions for the production of this enzyme were evaluated by response surface methodology. the optimal conditions to produce β-glucanase were a glucose concentration of 4% (w/v), a ph of 5.5, and an incubation temperature of 35 °c. response surface methodology was also used to determine the ph and temperature required for the optimal enzymatic activity. the highest enzyme activity wa ...201424390854
variation in phenolic compounds, anthocyanins, and color in red wine treated with enzymatic extract of kluyveromyces marxianus.the effect of the addition of enzymatic extract of kluyveromyces marxianus nrrl-y-7571 during the maceration and fermentation steps of cabernet sauvignon wine production was evaluated. the results obtained in the analytical determinations of the wines showed levels within the limits established by legislation and similar to values found in other studies. the results show that by adding the enzyme to the red wines these showed color characteristics considered to be superior to those of the contro ...201424347085
xylitol production at high temperature by engineered kluyveromyces marxianus.several recombinant kluyveromyces marxianus strains were constructed through overexpressing the neurospora crassa xylose reductase genes. yzj015, which maintained the original xylitol dehydrogenase gene, produced xylitol with the highest productivity (1.49 g l(-1) h(-1)) from 100 g l(-1) xylose at 42 °c. even at 45 °c, yzj015 was still able to produce 60.03 g l(-1) xylitol from 100 g l(-1) xylose with a productivity of 1.25 g l(-1)h(-1). in addition, for 20 rounds of cell recycling at 42 °c, yzj ...201424291795
optimization, kinetics, and modeling of inulinase production by k. marxianus var. marxianus.pressmud, a by-product from the sugarcane industry, was used as a carbon source for the production of inulinase in solid-state fermentation (ssf). statistical experimental designs were employed to screen the nutrients and optimize the media composition for the production of inulinase by kluyveromyces marxianus var. marxianus. eighteen various nutrients were selected for preliminary screening of production medium component by plackett-burman design (pbd) technique. five nutrients were found to be ...201424274017
selection of a thermotolerant kluyveromyces marxianus strain with potential application for cellulosic ethanol production by simultaneous saccharification and fermentation.the development of technologies for cellulosic ethanol production by simultaneous saccharification and fermentation (ssf) depends on the use of microorganisms with high fermentative rates and thermotolerance. in this study, the ability of five kluyveromyces marxianus strains to produce ethanol from glucose at 45 °c was investigated. the highest fermentative parameters were observed with k. marxianus nrrl y-6860, which was then further studied. an initial evaluation of the oxygen supply on ethano ...201424222495
simultaneous single-cell protein production and cod removal with characterization of residual protein and intermediate metabolites during whey fermentation by k. marxianus.cheese whey fermentation with kluyveromyces marxianus was carried out at 40 °c and ph 3.5 to examine simultaneous single-cell protein production and chemical oxygen demand (cod) removal, determine the fate of soluble whey protein and characterize intermediate metabolites. after 36 h of batch fermentation, the biomass concentration increased from 2.0 to 6.0 g/l with 55 % cod reduction (including protein), whereas soluble whey protein concentration decreased from 5.6 to 4.1 g/l. it was confirmed t ...201424185705
opuntia ficus-indica cladodes as feedstock for ethanol production by kluyveromyces marxianus and saccharomyces cerevisiae.the feasibility of ethanol production using an enzymatic hydrolysate of pretreated cladodes of opuntia ficus-indica (prickly pear cactus) as carbohydrate feedstock was investigated, including a comprehensive chemical analysis of the cladode biomass and the effects of limited aeration on the fermentation profiles and sugar utilization. the low xylose and negligible mannose content of the cladode biomass used in this study suggested that the hemicellulose structure of the o. ficus-indica cladode w ...201425248867
sustainable conversion of coffee and other crop wastes to biofuels and bioproducts using coupled biochemical and thermochemical processes in a multi-stage biorefinery concept.the environmental impact of agricultural waste from the processing of food and feed crops is an increasing concern worldwide. concerted efforts are underway to develop sustainable practices for the disposal of residues from the processing of such crops as coffee, sugarcane, or corn. coffee is crucial to the economies of many countries because its cultivation, processing, trading, and marketing provide employment for millions of people. in coffee-producing countries, improved technology for treat ...201425204861
analysis of the yeast short-term crabtree effect and its origin.the short-term crabtree effect is defined as the immediate occurrence of aerobic alcoholic fermentation in response to provision of a pulse of excess sugar to sugar-limited yeast cultures. here we have characterized ten yeast species with a clearly defined phylogenetic relationship. yeast species were cultivated under glucose-limited conditions, and we studied their general carbon metabolism in response to a glucose pulse. we generated an extensive collection of data on glucose and oxygen consum ...201425161062
genomic sequence of the yeast kluyveromyces marxianus cct 7735 (ufv-3), a highly lactose-fermenting yeast isolated from the brazilian dairy industry.here, we present the draft genome sequence of kluyveromyces marxianus cct 7735 (ufv-3), including the eight chromosomes and the mitochondrial genomic sequences.201425377712
draft genome sequence of kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate.we determined the genome sequence of a thermotolerant yeast, kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate, and the sequence provides further insights into the genomic differences between this strain and other reported k. marxianus strains. the genome described here is composed of 11,165,408 bases and has 4,943 protein-coding genes.201425059876
steam explosion treatment for ethanol production from branches pruned from pear trees by simultaneous saccharification and fermentation.this study investigated the production of ethanol from unutilized branches pruned from pear trees by steam explosion pretreatment. steam pressures of 25, 35, and 45 atm were applied for 5 min, followed by enzymatic saccharification of the extracted residues with cellulase (cellic ctec2). high glucose recoveries, of 93.3, 99.7, and 87.1%, of the total sugar derived from the cellulose were obtained from water- and methanol-extracted residues after steam explosion at 25, 35, and 45 tm, respectively ...201425036499
fine structure of tibetan kefir grains and their yeast distribution, diversity, and shift.tibetan kefir grains (tkgs), a kind of natural starter for fermented milk in tibet, china, host various microorganisms of lactic acid bacteria, yeasts, and occasionally acetic acid bacteria in a polysaccharide/protein matrix. in the present study, the fine structure of tkgs was studied to shed light on this unusual symbiosis with stereomicroscopy and thin sections. the results reveal that tkgs consist of numerous small grain units, which are characterized by a hollow globular structure with a di ...201424977409
deletion of a ku80 homolog enhances homologous recombination in the thermotolerant yeast kluyveromyces marxianus.targeted gene replacement in the thermotolerant yeast kluyveromyces marxianus kctc 17555 has been hampered by its propensity to non-homologous end joining (nhej). to enhance homologous recombination (hr) by blocking nhej, we identified and disrupted the k. marxianus ku80 gene. the ku80 deletion mutant strain (kmku80∆) of k. marxianus kctc 17555 did not show apparent growth defects under several conditions with the exception of exposure to tunicamycin. the targeted disruption of the three model g ...201424930110
sulfuric acid hydrolysis and detoxification of red alga pterocladiella capillacea for bioethanol fermentation with thermotolerant yeast kluyveromyces marxianus.one-step sulfuric acid saccharification of the red alga pterocladiella capillacea was optimized, and various detoxification methods (neutralization, overliming, and electrodialysis) of the acid hydrolysate were evaluated for fermentation with the thermotolerant yeast kluyveromyces marxianus. a proximate composition analysis indicated that p. capillacea was rich in carbohydrates. a significant galactose recovery of 81.1 ± 5% was also achieved under the conditions of a 12% (w/v) biomass load, 5% ( ...201424851812
mixed culture of kluyveromyces marxianus and candida krusei for single-cell protein production and organic load removal from whey.the study was conducted to evaluate the potential of mixed culture of kluyveromyces marxianus and candida krusei to enhance cod removal efficiency, minimize contamination at extreme conditions (high temperature 40°c and low ph 3.5) during batch and continuous aerobic fermentation and to obtain improved quality single-cell protein (scp) using whey as substrate. the batch fermentation of mono-culture and mixed culture result showed that the mixed culture resulted in 8.8% higher cod removal efficac ...201424844166
whole cells in enantioselective reduction of benzyl acetoacetate.the β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (s)-3-hydroxybutanoate by seven microorganism species. the best result using free cells was obtained with the yeast hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. after immobilization in calcium alginate spheres, k.marxianus showed to be more stable after 2 cycles of reaction.201425477927
biosynthesis of 2-phenylethanol from glucose with genetically engineered kluyveromyces marxianus.2-phenylethanol (2-pe) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-pe is produced in a few yeast strains by ehrlich pathway. in this study, kluyveromyces marxianus was genetically engineered for overproduction of 2-pe from glucose. about 1.0g/l of 2-pe was produced by overexpressing phenylpyruvate decarboxylase (aro10) and alcohol dehydrogenase (adh2) genes of saccharomyces cerevisiae. a similar level of 2-pe was also produced from e ...201424910335
neuro-fuzzy based model of batch fermentation of kluyveromyces marxianus var. lactis mc5.in this work a neuro-fuzzy based model of a whey batch fermentation process by a strain kluyveromyces marxianus var. lactis mc5 is presented. a three-layered neuro-fuzzy network is realized. the simulation results are compared with conventional models (based on mass balance and differential equations). the neuro-fuzzy model provides a better fitness and allows inclusion of linguistic variables (such as colour, smell, taste, morphophysiology, etc.). the accuracy is approximately equal to this ach ...201426019585
polymicrobial chronic endophthalmitis diagnosed by culture and molecular technique.accurate etiological diagnosis is the key to prevention of ocular morbidity in endophthalmitis cases. a 66 year old male was suffering from chronic endophthalmitis post-cataract surgery. polymerase chain reaction examination on anterior chamber fluid was positive for propionibacterium acnes but negative for the panfungal genome. he was advised vitrectomy with intravitreal injections. polymerase chain reaction of vitreous aspirate was positive for p.acnes as well as panfungal genome. the vitreous ...201425008833
production of folate in oat bran fermentation by yeasts isolated from barley and diverse foods.the focus of the research was to identify yeasts from barley kernels in order to study their folate production capability while maintaining high viscosity caused by soluble fibres in oat bran fermentation.201424916316
susceptibility of species within the sporothrix schenckii complex to a panel of killer yeasts.the sporothrix schenckii complex is the etiologic agent of sporotrichosis, a subacute or chronic mycosis which can affect humans and animals. killer yeasts have been used in the medical field for development of novel antimycotics and biotyping of pathogenic fungi. the action of 18 killer yeasts on the growth of 88 characterized s. schenckii, sporothrix globosa, sporothrix brasiliensis, and sporothrix mexicana clinical and environmental isolates was evaluated. killer studies were performed on pet ...201423686831
impact of microbial cultures on proteolysis and release of bioactive peptides in fermented milk.this study aimed at evaluating co-cultures of selected microorganisms for their proteolytic activity and capability to produce fermented milk enriched with ace-inhibitory (acei) peptides. selected yeasts (torulaspora delbruekii kl66a, galactomyces geotrichum kl20b, pichia kudriavzevii kl84a and kluyveromyces marxianus kl26a) and lactic acid bacteria strains (lactobacillus plantarum lat03, lb. plantarum klat01 and the not virulent enterococcus faecalis ke06) were screened as single cultures for t ...201424929726
alcohol dehydrogenases from kluyveromyces marxianus: heterologous expression in escherichia coli and biochemical characterization.kluyveromyces marxianus has recently become a species of interest for ethanol production since it can produce ethanol at high temperature and on a wide variety of substrates. however, the reason why this yeast can produce ethanol at high temperature is largely unknown.201424885162
non-homologous end joining-mediated functional marker selection for dna cloning in the yeast kluyveromyces marxianus.the cloning of dna fragments into vectors or host genomes has traditionally been performed using escherichia coli with restriction enzymes and dna ligase or homologous recombination-based reactions. we report here a novel dna cloning method that does not require dna end processing or homologous recombination, but that ensures highly accurate cloning. the method exploits the efficient non-homologous end-joining (nhej) activity of the yeast kluyveromyces marxianus and consists of a novel functiona ...201424307396
two missense mutations, e123q and k151e, identified in the erg11 allele of an azole-resistant isolate of candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia.we report on the first cloning and nucleotide sequencing of an erg11 allele from a clinical isolate of candida kefyr cross-resistant to azole antifungals. it was recovered from a stem cell transplant patient, in an oncohematology unit exhibiting unexpected high prevalence of c. kefyr. two amino acid substitutions were identified: k151e, whose role in fluconazole resistance was already demonstrated in candida albicans, and e123q, a new substitution never described so far in azole-resistant candid ...201424936404
[inulinases from various producers: the features of their permolecular organization].the structural organization of inulinases from yeasts, fungi, and plants are researched. for studying their sizes, molecular weight, and permolecular organization, an approach consisting of a combination of atomic force microscopy with methods of dynamic light scattering, gel chromatography, and electrophoresis was used. it is shown that inulinases from kluyveromyces marxianus and aspergillus niger form geterodimers and inulinases from tubers of helianthus tuberosus are present as both dimers an ...201425272747
direct fermentation of raw starch using a kluyveromyces marxianus strain that expresses glucoamylase and alpha-amylase to produce ethanol.raw starch and raw cassava tuber powder were directly and efficiently fermented at elevated temperatures to produce ethanol using the thermotolerant yeast kluyveromyces marxianus that expresses α-amylase from aspergillus oryzae as well as α-amylase and glucoamylase from debaryomyces occidentalis. among the constructed k. marxianus strains, yrl 009 had the highest efficiency in direct starch fermentation. raw starch from corn, potato, cassava, or wheat can be fermented at temperatures higher than ...201424478139
uncommon opportunistic yeast bloodstream infections from qatar.eleven uncommon yeast species that are associated with high mortality rates irrespective of antifungal therapy were isolated from 17/187 (201 episodes) pediatric and elderly patients with fungemia from qatar. the samples were taken over a 6-year period (january 2004-december 2010). isolated species included kluyveromyces marxianus, lodderomyces elongisporus, lindnera fabianii, candida dubliniensis, meyerozyma guilliermondii, candida intermedia, pichia kudriavzevii, yarrowia lipolytica, clavispor ...201424934803
diversity of culturable yeasts in phylloplane of sugarcane in thailand and their capability to produce indole-3-acetic acid.yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in thailand. all sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. on the basis of the d1/d2 domain of the large subunit rrna gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the ascomycota viz. candida akabanensis, candida dendronema, candida mesorugosa, candida michaelii, candida nivarien ...201424442819
the predominance, biodiversity and biotechnological properties of kluyveromyces marxianus in the production of pecorino di farindola cheese.pecorino di farindola is a handicraft cheese made by farmers on small scale using raw ewes' milk and pig rennet. in this study, yeast consortia were evaluated during pecorino di farindola making and ripening. molecular identification of 156 isolates was achieved by a combination of pcr-rflp of the 5.8s its rrna region and sequencing of the d1/d2 domain of the 26s rrna gene. kluyveromyces marxianus was the predominant species, while other species (pichia kudriavzevii, candida parapsilosis, candid ...201425038503
selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting.the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ...201426139877
yeasts are essential for cocoa bean fermentation.cocoa beans (theobroma cacao) are the major raw material for chocolate production and fermentation of the beans is essential for the development of chocolate flavor precursors. in this study, a novel approach was used to determine the role of yeasts in cocoa fermentation and their contribution to chocolate quality. cocoa bean fermentations were conducted with the addition of 200ppm natamycin to inhibit the growth of yeasts, and the resultant microbial ecology and metabolism, bean chemistry and c ...201424462702
microbial diversity and dynamics during the production of may bryndza cheese.diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ...201424291178
influencing cocoa flavour using pichia kluyveri and kluyveromyces marxianus in a defined mixed starter culture for cocoa fermentation.the potential impact of aromatic and pectinolytic yeasts on cocoa flavour was investigated using two defined mixed starter cultures encompassing strains of pichia kluyveri and kluyveromyces marxianus for inoculating cocoa beans in small scale tray fermentations. samples for microbial and metabolite analysis were collected at 12-24 hour intervals during 120 h of fermentation. yeast isolates were grouped by (gtg)5-based rep-pcr fingerprinting and identified by sequencing of the d1/d2 region of the ...201323866910
candida utilis and chlorella vulgaris counteract intestinal inflammation in atlantic salmon (salmo salar l.).intestinal inflammation, caused by impaired intestinal homeostasis, is a serious condition in both animals and humans. the use of conventional extracted soybean meal (sbm) in diets for atlantic salmon and several other fish species is known to induce enteropathy in the distal intestine, a condition often referred to as sbm induced enteropathy (sbmie). in the present study, we investigated the potential of different microbial ingredients to alleviate sbmie in atlantic salmon, as a model of feed-i ...201324386162
assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for cbp ethanol production.many microorganisms possess enzymes that can efficiently degrade lignocellulosic materials, but do not have the capability to produce a large amount of ethanol. thus, attempts have been made to transform such enzymes into fermentative microbes to serve as hosts for ethanol production. however, an efficient host for a consolidated bioprocess (cbp) remains to be found. for this purpose, a synthetic biology technique that can transform multiple genes into a genome is instrumental. moreover, a strat ...201323374631
diversity of fungal flora in raw milk from the italian alps in relation to pasture altitude.the present paper explores the diversity of mycobiota inhabiting raw milk sampled at different altitudes (1400 m, 1800 m, 2200 m) from cows grazing alpine pastures of valle d'aosta (north-western italian alps). to this aim, multilocus sequencing was performed at barcodes commonly used for fungal identification (its1, d1/d2 domains of the 26s rrna gene, and part of the β-tubulin gene). a total of 31 species were detected, most of them yeasts, followed by moulds and by 2 sequences of macroscopic f ...201324024093
yeast dynamics during spontaneous fermentation of mawè and tchoukoutou, two traditional products from benin.mawè and tchoukoutou are two traditional fermented foods largely consumed in benin, west africa. their preparations remain as a house art and they are the result of spontaneous fermentation processes. in this study, dynamics of the yeast populations occurring during spontaneous fermentations of mawè and tchoukoutou were investigated using both culture-dependent and -independent approaches. for each product, two productions were followed. samples were taken at different fermentation times and yea ...201323756236
role of hydrophobicity in adhesion of wild yeast isolated from the ultrafiltration membranes of an apple juice processing plant.the role of cell surface hydrophobicity in the adhesion to stainless steel (ss) of 11 wild yeast strains isolated from the ultrafiltration membranes of an apple juice processing plant was investigated. the isolated yeasts belonged to four species: candida krusei (5 isolates), candida tropicalis (2 isolates), kluyveromyces marxianus (3 isolates) and rhodotorula mucilaginosa (1 isolate). surface hydrophobicity was measured by the microbial adhesion to solvents method. yeast cells and surfaces were ...201323837866
determination of yeast diversity in ogi, mawè, gowé and tchoukoutou by using culture-dependent and -independent methods.the maize based ogi and mawè and the sorghum based gowé and tchoukoutou are traditional, spontaneously fermented products widely consumed by the population of benin (west africa). yeast occurrence in the products, as sold on local markets at different locations, was studied using a combination of culture-dependent and independent methods. number of yeasts is varied from 3.75 log10 colony forming units (cfu)/g for ogi to 5.60 log10 cfu/g for tchoukoutou. isolated yeasts (236) were identified base ...201323727651
yeasts from autochthonal cheese starters: technological and functional properties.the aim of this work was to identify 20 yeasts isolated from autochthonal cheese starters and evaluate their technological and functional properties.201323600736
protective effect of a mixture of kefir-isolated lactic acid bacteria and yeasts in a hamster model of clostridium difficile infection.the objective of this work was to test the protective effect of a mixture (mm) constituted by kefir-isolated microorganisms (lactobacillus plantarum, lactobacillus kefir, lc. lactis, kluyveromyces marxianus and saccharomyces cerevisiae) in a hamster model of infection with clostridium difficile, an anaerobic gram-positive bacterium that causes diarrhoea. placebo or mm was administered ad libitum in drinking water from day 0 to the end of treatment. hamsters received orally 200 μg of clyndamicin ...201323542116
kefir-isolated lactococcus lactis subsp. lactis inhibits the cytotoxic effect of clostridium difficile in vitro.kefir is a dairy product obtained by fermentation of milk with a complex microbial population and several health-promoting properties have been attributed to its consumption. in this work, we tested the ability of different kefir-isolated bacterial and yeast strains (lactobacillus kefir, lb. plantarum, lactococcus lactis subps. lactis, saccharomyces cerevisiae and kluyveromyces marxianus) or a mixture of them (mm) to antagonise the cytopathic effect of toxins from clostridium difficile (tcda and ...201323217732
identification and assessment of kefir yeast potential for sugar/ethanol-resistance.biochemical and molecular analysis was used for identification of different kefir yeasts species from brazil, canada and the united states of america. the sugar/ethanol-resistant activity of the yeasts was evaluated. saccharomyces cerevisiae and kluyveromyces marxianus had the highest growth rates, suggesting biotechnological applications possible for these strains.201324159292
identification of auxotrophic mutants of the yeast kluyveromyces marxianus by non-homologous end joining-mediated integrative transformation with genes from saccharomyces cerevisiae.the isolation and application of auxotrophic mutants for gene manipulations, such as genetic transformation, mating selection and tetrad analysis, form the basis of yeast genetics. for the development of these genetic methods in the thermotolerant fermentative yeast kluyveromyces marxianus, we isolated a series of auxotrophic mutants with defects in amino acid or nucleic acid metabolism. to identify the mutated genes, linear dna fragments of nutrient biosynthetic pathway genes were amplified fro ...201324150815
biosorption of lead by kluyveromyces marxianus immobilized in alginate beads.the uptake and recovery of pb (ii) ions were investigated by using sodium alginate beads. biosorption experiments are carried out in batch mode. the experimental results showed that the beads were effective in removing pb (ii) ions from solution. biosorption equilibrium was approached within 16hrs. pseudo first order was applicable to all the sorption data over the entire time range. the sorption data conformed well for the langmuir isotherm model. the maximum adsorption capacity (qmax) onto alg ...201324558794
growth of kluyveromyces marxianus and formation of ethyl acetate depending on temperature.conversion of lactose into ethyl acetate by kluyveromyces marxianus allows economic reuse of whey-borne sugar. the high volatility of ethyl acetate enables its process-integrated recovery by stripping. this stripping is governed by both the aeration rate and the partition coefficient, k ea,l/g. cultivation at elevated temperatures should decrease the k ea,l/g value and thus favor stripping. k. marxianus dsm 5422 as a potent producer of ethyl acetate was cultivated aerobically in whey-borne media ...201324104467
fermentative production of ribonucleotides from whey by kluyveromyces marxianus: effect of temperature and ph.ribonucleotides have shown many promising applications in food and pharmaceutical industries. the aim of the present study was to produce ribonucleotides (rna) by kluyveromyces marxianus atcc 8,554 utilizing cheese whey, a dairy industry waste, as a main substrate under batch fermentation conditions. the effects of temperature, ph, aeration rate, agitation and initial cellular concentration were studied simultaneously through factorial design for rna, biomass production and lactose consumption. ...201324426003
simultaneous saccharification and fermentation of agave tequilana fructans by kluyveromyces marxianus yeasts for bioethanol and tequila production.agave tequilana fructans (atf) constitute a substrate for bioethanol and tequila industries. as kluyveromyces marxianus produces specific fructanases for atf hydrolysis, as well as ethanol, it can perform simultaneous saccharification and fermentation. in this work, fifteen k. marxianus yeasts were evaluated to develop inoculums with fructanase activity on atf. these inoculums were added to an atf medium for simultaneous saccharification and fermentation. all the yeasts, showed exo-fructanhydrol ...201323941710
impact of high temperature on ethanol fermentation by kluyveromyces marxianus immobilized on banana leaf sheath pieces.ethanol fermentation was carried out with kluyveromyces marxianus cells at various temperatures (30, 35, 40, and 45 °c). fermentation performance of the immobilized yeast on banana leaf sheath pieces and the free yeast were evaluated and compared. generally, ethanol production of the immobilized and free yeast was stable in a temperature range of 30-40 °c. temperature of 45 °c restricted yeast growth and lengthened the fermentation. the immobilized yeast demonstrated faster sugar assimilation an ...201323900619
ethanol fermentation from xylose by metabolically engineered strains of kluyveromyces marxianus.we constructed a xylose-fermenting recombinant strain of thermotolerant yeast kluyveromyces marxianus, dmb3-7. both xylose consumption and ethanol production were remarkably increased in dmb3-7 compared to the control strain at 30°c. furthermore, dmb3-7 produced ethanol from xylose at both 42°c and 45°c, above which xylose metabolic activity decreased.201323871200
simultaneous integration of multiple genes into the kluyveromyces marxianus chromosome.while kluyveromyces marxianus is a promising yeast strain for biotechnological applications, genetic engineering of this strain is still challenging, especially when multiple genes are to be transformed. sequential gene integration, which takes advantage of repetitive insertion/excision of the ura3 gene as a marker, has been the best option until now, because the ura3-deletion mutant is the only precondition for this method. however, we found that the introduced gene is co-excised during the ura ...201323845272
bioethanol production from lignocellulosic biomass by a novel kluyveromyces marxianus strain.the yeast kluyveromyces marxianus is considered as a potential alternative to saccharomyces cerevisiae in producing ethanol as a biofuel. in this study, we investigated the ethanol fermentation properties of novel k. marxianus strain dmb1, isolated from bagasse hydrolysates. this strain utilized sorbitol as well as various pentoses and hexoses as single carbon sources under aerobic conditions and produced ethanol from glucose in hydrolysates of the japanese cedar at 42 °c. reference strains k. m ...201323832346
bioprospecting of thermo- and osmo-tolerant fungi from mango pulp-peel compost for bioethanol production.the persistent edaphic stress on microbial succession due to dynamic changes during composting was explored for selection of multi-stress tolerant microbe(s) desirable for ethanol production. a total of 23 strains were isolated from mango compost using four successive enrichments in yp broth (g l(-1)): glucose, 100; 150; 250 with ethanol (40) and cycloheximide (0.4) at 40 °c, ph 6.0. based on multi-gene ribotyping, 14 yeasts (61 %) of saccharomycetaceae, 2 filamentous fungi (8.6 %) and 7 bacteri ...201323180376
formation of ethyl acetate from whey by kluyveromyces marxianus on a pilot scale.whey arising in huge amounts during milk processing is a valuable renewable resource in the field of white biotechnology. kluyveromyces marxianus is able to convert whey-borne lactose into ethyl acetate, an environmentally friendly solvent. formation of ethyl acetate as a bulk product is triggered by iron (fe). k. marxianus dsm 5422 was cultivated aerobically in whey-borne medium originally containing 40 μg/l fe, supplemented with 1, 3 or 10 mg/l fe in the pre-culture, using an 1 l or 70 l stirr ...201323089728
[effect of aeration and inulin concentration on ethanol production by kluyveromyces marxinaus yx01].consolidated bioprocessing technology can be used for kluyveromyces marxianus yx01 to produce ethanol from jerusalem artichoke, which is one of the potential processes to produce biofuel from non-cereal crops. in this study, we combined the aeration rate with the substrate concentration to conduct cross-over experiments for k. marxinaus yx01, and studied ethanol fermentation and the influence of inulin enzyme activity. the substrate concentration had a little repressive effect on ethanol product ...201323789273
discarded oranges and brewer's spent grains as promoting ingredients for microbial growth by submerged and solid state fermentation of agro-industrial waste mixtures.the exploitation of various agro-industrial wastes for microbial cell mass production of kluyveromyces marxianus, kefir, and saccharomyces cerevisiae is reported in the present investigation. specifically, the promotional effect of whole orange pulp on cell growth in mixtures consisting of cheese whey, molasses, and potato pulp in submerged fermentation processes was examined. a 2- to 3-fold increase of cell mass was observed in the presence of orange pulp. likewise, the promotional effect of br ...201323780341
expression of exoinulinase genes in saccharomyces cerevisiae to improve ethanol production from inulin sources.to improve inulin utilization and ethanol fermentation, exoinulinase genes from the yeast kluyveromyces marxianus and the recently identified yeast, candida kutaonensis, were expressed in saccharomyces cerevisiae. s. cerevisiae harboring the exoinulinase gene from c. kutaonensis gave higher ethanol yield and productivity from both inulin (0.38 vs. 0.34 g/g and 1.35 vs. 1.22 g l(-1) h(-1)) and jerusalem artichoke tuber flour (0.47 vs. 0.46 g/g and 1.62 vs. 1.54 g l(-1) h(-1)) compared with the st ...201323743955
inulinase production in a packed bed reactor by solid state fermentation.in this work, production of inulinase was carried out in a packed bed reactor (pbr) under solid state fermentation. kluyveromyces marxianus var. marxianus was used to produce the inulinase using pressmud as substrate. the parameters like air flow rate, packing density and particle size were optimized using response surface methodology (rsm) to maximize the inulinase production. the optimum conditions for the maximum inulinase production were: air flow rate - 0.82 l/min, packing density - 40 g/l ...201323688470
improved xylose fermentation of kluyveromyces marxianus at elevated temperature through construction of a xylose isomerase pathway.to improve the xylose fermentation ability of kluyveromyces marxianus, a xylose assimilation pathway through xylose isomerase was constructed. the genes encoding xylose reductase (kmxyl1) and xylitol dehydrogenase (kmxyl2) were disrupted in k. marxianus yhj010 and the resultant strain was named yrl002. a codon-optimized xylose isomerase gene from orpinomyces was transformed into k. marxianus yrl002 and expressed under gapdh promoter. the transformant was adapted in the sd medium containing 1 % c ...201323657586
evaluation of hardboard manufacturing process wastewater as a feedstream for ethanol production.waste streams from the wood processing industry can serve as feedstream for ethanol production from biomass residues. hardboard manufacturing process wastewater (hpw) was evaluated on the basis of monomeric sugar recovery and fermentability as a novel feedstream for ethanol production. dilute acid hydrolysis, coupled with concentration of the wastewater resulted in a hydrolysate with 66 g/l total fermentable sugars. as xylose accounted for 53 % of the total sugars, native xylose-fermenting yeast ...201323604526
thermal adaptability of kluyveromyces marxianus in recombinant protein production.kluyveromyces marxianus combines the ease of genetic manipulation and fermentation with the ability to efficiently secrete high molecular weight proteins, performing eukaryotic post-translational modifications. it is able to grow efficiently in a wide range of temperatures. the secretion performances were analyzed in the host k. marxianus l3 in the range between 5°c and 40°c by means of 3 different reporter proteins, since temperature appears a key parameter for production and secretion of recom ...201323587421
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