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interactions between various microbes and ginseng botanicals.three kinds of interactions occur between ginseng botanicals and microorganisms: a) spoilage of the botanical by various fungi (e.g., aspergillus, penicillium, alternaria, and eurotium species) and bacteria; b) transformation of ginsenosides into more bioactive forms by bacteria such as intrasporangium sp. gs603, microbacterium sp. gs514, caulobacter leidyia, bifidobacterium sp. int57, bifidobacterium sp. sj32, fusobacterium sp. and bacteroides sp., and moulds (e.g., aspergillus niger, fusarium ...201121254831
antifungal activity and mechanism of fengycin in the presence and absence of commercial surfactin against rhizopus stolonifer.the antifungal activity and mechanism of fengycin in the presence and absence of commercial surfactin against rhizopus stolonifer were investigated. the mic (minimal inhibitory concentration) of fengycin without commercial surfactin added was 0.4 mg/ml while the mic of fengycin with commercial surfactin added was 2.0 mg/ml. fengycin acted on cell membrane and cellular organs and inhibited dna synthesis. the antifungal effect of fengycin was reduced after commercial surfactin was added. all these ...201121369992
molecular cloning and expression analysis of a delta 6-fatty acid desaturase gene from rhizopus stolonifer strain yf6 which can accumulate high levels of gamma-linolenic acid.the delta 6-desaturase gene was cloned from rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (gla, c18:3 ?(6,9,12)) to the total fatty acids. the cloned dna contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and hpgg motif. notably, this deduced sequence had a shorter c-terminus. results demonstrated that the cdna sequence exhibite ...201121369993
antifungal activity of essential oil from asteriscus graveolens against postharvest phytopathogenic fungi in apples.the essential oils of the aerial parts of asteriscus graveolens have been studied using gc and gc-ms. twenty-eight compounds were identified in the essential oil amounting to 94.9% of the total oil. the aerial part oils showed similar chromatographic profiles and were characterized by having a high content of oxygenated sesquiterpenes with 6-oxocyclonerolidol (66.7% +/- 5.5) and 6-hydroxycyclonerolidol (8.8% +/-1.2) as the major components. the antifungal effect of the essential oil from a. grav ...201122224306
Liriodenine, early antimicrobial defence in Annona diversifolia.Annonaceae aporphine alkaloids, of which liriodenine is the most abundant, have not been extensively studied from a biological standpoint. The goal of this study was to investigate the role of liriodenine in antimicrobial defense during early developmental stages in Annona diversifolia. The fungi Rhizopus stolonifer and Aspergillus glaucus, which are responsible for seed deterioration, were isolated during imbibition, and their antifungal activity was determined by diffusion, macrodilution, and ...201121950162
antifungal activity in ethanolic extracts of carica papaya l. cv. maradol leaves and seeds.bioactive compounds from vegetal sources are a potential source of natural antifungic. an ethanol extraction was used to obtain bioactive compounds from carica papaya l. cv. maradol leaves and seeds of discarded ripe and unripe fruit. both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. the effect of time on extraction efficiency was confirmed by qualitative identif ...201122282629
influence of fungal elicitation on glycyrrhizin production in transformed cell cultures of abrus precatorius linn.glycyrrhizin, obtained from abrus precatorius (indian liquorice), is a phytoconstituent of importance for pharmaceutical and food industries.201122262933
currency notes and coins as a possible source of transmitting fungal pathogens of man and plants.currency (notes and coins) handling by people during transaction is one of the most mobile objects within the community, which has a potential of transmitting pathogens. a survey carried out recently in nanded city (maharashtra) revealed heavy contamination of currency notes and coins by important fungal pathogens of plants and man, i.e. aspergillus niger (60.37%), a. flavus (3.98%), a.nidulans (0.2%), penicillium citrinum (17.80%), alternaria tenuis (0.20%), curvularia pallescens (0.20%), clado ...201123505834
natural resistance of rose petals to microbial attack.petals of red, yellow and white roses (rosa damascene mill.) of the family rosaceae were extracted with (1:1) methylene chloride/methanol and tested for their antimicrobial activities against four species of gram-positive bacteria (bacillus cereus, bacillus subtilis, micrococcus luteus and staphylococcus aureus), five species of gram-negative bacteria (enterobacter aerogenes, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa and serratia marcescens) and five species of fungi (penici ...201121614712
antagonistic action of bacillus subtilis strain fmbj on the postharvest pathogen rhizopus stolonifer.the antagonistic activities of bacillus subtilis fmbj against the rhizopus stolonifer pathogen causing peach soft rot disease were studied in this paper. bacillus subtilis fmbj exhibited a high antifungal effect on the mycelium growth, sportulation, and germ tube elongation of r. stolonifer with the inhibition rate of 86.5% and 97.42%, respectively, spore germination rate of 20% under antagonist strain concentration of 10⁸ cfu/ml. by using of scanning electron microscope (sem) and transmission e ...201122417435
molecular characterization of mucor circinelloides and rhizopus stolonifer strains isolated from some saudi fruits.thirteen strains of mucor circinelloides and nine strains of rhizopus stolonifer were collected from different saudi fruits. also, two strains of m. circinelloides and one strain of r. stolonifer isolated from soil were used in this study to analyze the genetic diversity among these 25 strains. the dendrogram constructed from random amplified polymorphic dna-polymerase chain reaction results using two primers (v6 and m13) showed no correlation between clustering system and sources of isolation f ...201019877768
a combination of heat treatment and pichia guilliermondii prevents cherry tomato spoilage by fungi.this study investigated the effectiveness of heat treatment and pichia guilliermondii, either alone or in combination, to combat postharvest fungal spoilage in cherry tomato fruit. in vitro experiments demonstrated that heat treatment at 38 degrees c significantly inhibited mycelial growth of three different pathogens (botrytis cinerea, alternaria alternata and rhizopus stolonifer ehrenb). in vivo experiments unveiled that either heat treatment or p. guilliermondii reduced decay caused by these ...201019923029
[7alpha-hydroxylation of steroid 5-olefins by mold fungi].hydroxylation activity of the mold fungi belonging to the orders dothideales, hypocreales, and mucorales towards delta(5)-3beta-hydroxysteroids was studied. the fungi bipolaris sorokiniana, fusarium sp., and rhizopus nigricans were able to introduce hydroxy group at position 7alpha; however, this ability was detected only at a low substrate load and with a low yield. a 7alpha-hydroxylase activity of the curvularia lunata vkpm f-981 culture was shown for the first time. it was demonstrated that t ...201020198922
biocrystallization of silver and gold ions by inactive cell filtrate of rhizopus stolonifer.microbes and their cell filtrates are known to synthesize metal nanoparticles. but maintenance of aseptic conditions and irregularly shaped and sized nanoparticles are major drawbacks of the system. in this study cell filtrate from inactive biomass of rhizopus stolonifer was used for the first time to produce near uniformly sized and shaped ag and au nanoparticles at room temperature. the size of ag and au nanoparticles were found in the range of 25-30 nm and 1-5 nm, respectively. uv-vis spectru ...201020627484
ntkti1, a kunitz trypsin inhibitor with antifungal activity from nicotiana tabacum, plays an important role in tobacco's defense response.a cdna library from tobacco inoculated with rhizoctonia solani was constructed, and several cdna fragments were identified by differential hybridization screening. one cdna clone that was dramatically repressed, ntkti1, was confirmed as a member of the kunitz plant proteinase inhibitor family. rt-pcr analysis revealed that ntkti1 was constitutively expressed throughout the whole plant and preferentially expressed in the roots and stems. furthermore, rt-pcr analysis showed that ntkti1 expression ...201020735473
isolation and identification of microbes associated with mobile phones in dammam in eastern saudi arabia.this study was conducted to determine microbial contamination of mobile phones in the city of dammam, in the eastern region of saudi arabia, and identify the most important microbial species associated with these phones in order to take the necessary remedial measures.201022022665
an attempt of postharvest orange fruit rot control using essential oils from mediterranean plants.twelve essential oils from mediterranean aromatic plants were tested at different doses against four fungi known as causal agents of post-harvest orange fruit rot: botrytis cinerea, penicillium italicum, phytophthora citrophthora, and rhizopus stolonifer. essential oils were obtained from hyssopus officinalis, lavandula angustifolia, majorana hortensis, melissa officinalis, ocimum basilicum, origanum vulgare, salvia officinalis, and thymus vulgaris (family lamiaceae), verbena officinalis (family ...201020874226
influence of growth regulators and elicitors on cell growth and α-tocopherol and pigment productions in cell cultures of carthamus tinctorius l.the present study examined the effects of plant growth hormones, incubation period, biotic (trametes versicolor, mucor sp., penicillium notatum, rhizopus stolonifer, and fusarium oxysporum) and abiotic (nacl, mgso(4), feso(4), znso(4), and fecl(3)) elicitors on cell growth and α-tocopherol and pigment (red and yellow) productions in carthamus tinctorius cell cultures. the cell growth and α-tocopherol and pigment contents improved significantly on murashige and skoog (ms) liquid medium containing ...201021120469
identification and partial characterization of rhizopus nigricans gβ proteins and their expression in the presence of progesterone.the mammalian steroid hormone progesterone actuates a signalling pathway in the zygomycete rhizopus nigricans which includes heterotrimeric g proteins. to investigate the possibility that the gβ subunit of these proteins is involved in the signalling, a cdna library from r. nigricans exposed to progesterone was prepared and a sequence coding for a gβ subunit was searched for. using degenerate primers, two sequences, rngpb1 and rngpb2, were identified that exhibited a high degree of identity with ...201021195176
screening filamentous tropical fungi for their nutritional potential as sources of crude proteins, lipids and minerals.the use of fungal and yeast biomass in foodstuff, either as supplements or as major ingredients in formulations is an area of growing interest for the modern food industry. the aim of this study was to evaluate the nutritional potential of biomasses obtained from filamentous fungi penicillium sclerotiorum, penicillium janthinellum, rhizopus stolonifer and syncephalastrum racemosum. biomasses presented 26-37% of total proteins, 1.7-3.5% of lipids and 4.6-9.1% of ashes. the humidity level reached ...201021339148
screening of functional rhizopus stolonifer for alcohol fermentation and production of high quality korean traditional rice wine.different strains of mold were screened for the production of high quality korean traditional rice wine with anti-hypertension and good acceptability. we isolated 867 nuruk mold strains and selected 24 for further study based on measurement of amylase activity. among them, mold no. 17 showed high ethanol production upon fermentation with saccharomyces cerevisiae as well as anti-hypertensive properties. the no. 17 strain was therefore selected as the functional mold and later identified as rhizop ...201023956639
novel agents for enzymatic and fungal hydrolysis of stevioside.a comparative study on the potential of some biological agents to perform the hydrolysis of stevioside was carried out, aiming at establishing an alternative methodology to achieve the aglycon steviol or its rearranged derivative isosteviol, in high yields to be used in the preparation of novel bioactive compounds. hydrolysis reactions were performed by using filamentous fungi (aspergillus niger, rhizopus stolonifer and rhizopus arrhizus), a yeast (saccharomyces cerevisiae) and enzymes (pancreat ...200924031374
antifungal activities of copper(ii) with biosensitive macrocyclic schiff base ligands derived from 4-aminoantipyrine derivatives.novel copper(ii) complexes have been synthesized from the macrocyclic schiff bases derived from knoevenagel condensed β-ketoanilides (obtained by the condensation of acetoacetanilide and substituted benzaldehydes), 4-aminoantipyrine and o-phenylene diamine. the structural features have been determined from their analytical and spectral data. all the cu(ii) complexes exhibit square planar geometry. their high molar conductance values support their 1: 2 electrolytic nature. the magnetic moment dat ...200923983523
a new selective medium for the recovery and enumeration of monilinia fructicola, m. fructigena, and m. laxa from stone fruits.isolation of monilinia spp. from stone and pome fruit surfaces is difficult due to the presence of several fast-growing fungal species such as rhizopus, alternaria, and penicillium spp. therefore, a new selective medium (acidified potato dextrose agar [ph 3.6] amended with fosetyl-aluminum [fosetyl-al] at 500 microg/ml) (apda-f500) was developed for the recovery of monilinia propagules. the antifungal agents fosetyl-al, dichloran, ammonium molybdate, and 2-deoxy-d-glucose (2-dd-glucose) were tes ...200919740034
identification and characterization of a novel delta6-fatty acid desaturase gene from rhizopus nigricans.fatty acid composition of fungi is analysed through the gas chromatography technique. with specific activity a novel enzyme delta6-fatty acid desaturase was screened and isolated from rhizopus nigricans. in this study r. nigricans was identified as a fungal species that produced plentiful gamma-linolenic acid. a 1,475 bp full-length cdna, designated as rnd6d here, with high homology to fungal delta6-fatty acid desaturase genes was isolated from r. nigricans using reverse transcription polymerase ...200919152021
[relationship of zn content and na/k ratio to the resistance to pathogen invasion in antisense acs transgenic tomato fruit and normal tomato].in the present work, inductively coupled plasma atomic emission spectrometry (icp-aes) was used to determine the content of zn, na and k and the ratio of na/k in antisense acs transgenic tomato fruit (in which the antisense acc synthase gene construct was inserted) and in normal tomato fruit of the same variety (lycopersicon esculentum cv. lichun). the difference in mineral elements content between the two tomatoes and the involvement of zn content and na/k ratio in the disease resistance of pos ...200919445235
evaluation of food additives as alternative or complementary chemicals to conventional fungicides for the control of major postharvest diseases of stone fruit.to evaluate potential alternatives to conventional fungicides to control decay, more than 20 food additives and generally regarded as safe compounds were tested at three concentrations in in vivo primary screenings with several cultivars of california peaches, nectarines, and plums that had been artificially inoculated with seven major postharvest pathogens: monilinia fructicola, botrytis cinerea, geotrichum candidum, alternaria alternata, penicillium expansum, mucor piriformis, and rhizopus sto ...200919517732
rapid detection and quantification of aspergillus fumigatus in environmental air samples using solid-phase cytometry.aspergillus fumigatus is an ubiquitous fungus capable of causing severe infections such as aspergilloma, allergic bronchopulmonary aspergillosis, and invasive aspergillosis, especially in immunocompromised patients. monitoring the number of aspergillus fumigatus spores in the air is crucial for infection control. in the present study, a novel approach for the quantification of aspergillus fumigatus, based on solid-phase cytometry (spc) and immunofluorescent labeling, was developed. the sensitivi ...200919534140
heat treatment in combination with antagonistic yeast reduces diseases and elicits the active defense responses in harvested cherry tomato fruit.this study investigated the effects of heat treatment (hot air at 38 degrees c) and antagonistic yeast (pichia guilliermondii) alone or in combination against postharvest diseases (botrytis cinerea, alternaria alternata and rhizopus nigricans) on cherry tomato fruit, and evaluated the elicitation of active defense responses. results showed that heat treatment at 38 degrees c for 24 h in combination with p. guilliermondii at 1 x 10(8) cfu ml(-1) was the most effective approach to reduce various i ...200919637930
cloning and expression of cellulase gene eg1 from rhizopus stolonifer var. reflexus tp-02 in escherichia coli.a novel gene, eg encoding enzymes involved in carboxymethyl cellulose (cmc) degradation was isolated, sequenced from the filamentous fungus rhizopus stolonifer var. reflexus tp-02, and expressed in escherichia coli bl21. the results showed that the gene amplified from the cdna of the strain could be classified as the family of endoglucanase. during the fermentation process, the maximum endoglucanase activity (i.e. 0.715 iu/ml) of the recombinant bacteria was obtained at 36 h. the sds-page analys ...200919640700
new oxandrolone derivatives by biotransformation using rhizopus stolonifer.structural transformation of the steroidal lactone, oxandrolone (1), by suspended-cell cultures of the plant pathogen fungus rhizopus stolonifer, resulted in the production of three new metabolites. these metabolites were identified as 11alpha-hydroxyoxandrolone (2), 6alpha-hydroxyoxandrolone (3) and 9alpha-hydroxyoxandrolone (4), by different spectroscopic methods and single-crystal x-ray diffraction analysis for metabolite 2. compounds 1 and 3 showed a significant beta-glucuronidase inhibitory ...200919698730
sporicidal activity of synthetic antifungal undecapeptides and control of penicillium rot of apples.the antifungal activity of cecropin a(2-8)-melittin(6-9) hybrid undecapeptides, previously reported as active against plant pathogenic bacteria, was studied. a set of 15 sequences was screened in vitro against fusarium oxysporum, penicillium expansum, aspergillus niger, and rhizopus stolonifer. most compounds were highly active against f. oxysporum (mic < 2.5 microm) but were less active against the other fungi. the best peptides were studied for their sporicidal activity and for sytox green upt ...200919617390
biosorption of simulated dyed effluents by inactivated fungal biomasses.treatment of dyed effluents presents several problems mainly due to the toxicity and recalcitrance of dyestuffs. innovative technologies, such as biosorption, are needed as alternatives to conventional methods to find inexpensive ways of removing dyes from large volumes of effluents. inactivated biomasses do not require a continuous supply of nutrients and are not sensitive to the toxicity of dyes or toxic wastes. they can also be regenerated and reused in many cycles and are both safe and envir ...200817888654
quantifying fungal viability in air and water samples using quantitative pcr after treatment with propidium monoazide (pma).a method is described to discriminate between live and dead cells of the infectious fungi aspergillus fumigatus, aspergillus flavus, aspergillus terreus, mucor racemosus, rhizopus stolonifer and paecilomyces variotii. to test the method, conidial suspensions were heat inactivated at 85 degrees c or held at 5 degrees c (controls) for 1 h. polycarbonate filters (25 mm diameter, 0.8 microm pore size) were placed on "welled" slides (14 mm diameter) and the filters treated with either pbs or pma. pro ...200818160156
high-affinity iron permease (ftr1) gene sequence-based molecular identification of clinically important zygomycetes.the clinical importance of zygomycosis, an emerging and frequently fatal mycotic disease, has increased during recent years. this report describes an identification method based on pcr amplification and sequencing of the high-affinity iron permease 1 gene (ftr1). primers and amplification protocols were established and tested for the identification of rhizopus oryzae, rhizopus microsporus var. rhizopodiformis, r. microsporus var. oligosporus, rhizopus schipperae, rhizopus niveus and rhizopus sto ...200818190575
is allergenic similarity predictable in respiratory allergies?first degree relatives of patients with allergic diseases are at increased risk of having the disorder. however, it is not clear whether two such related patients with allergic diseases are sensitive to the same antigens or not.200818479656
growth of phytopathogenic fungi in the presence of partially acetylated chitooligosaccharides.four phytopathogenic fungi were cultivated up to six days in media containing chitooligosaccharide mixtures differing in average dp and fa. the three different mixtures were named q3 (which contained oligosaccharides of dp2-dp10, with dp2-dp7 as main components), q2 (which contained oligosaccharides of dp2-dp12, with dp2-dp10 as main components) and q1 (which derived from q2 and contained oligomers of dp5-dp8 with hexamer and a heptamer as the main components). the novel aspect of this work is t ...200818523865
headspace sorptive extraction and gc-tofms for the identification of volatile fungal metabolites.volatiles from fungi cultivated in petri dishes were collected by a simple headspace polydimethylsiloxane (pdms) sorptive extraction technique (hsse), thermally desorbed into a gas chromatographic capillary column and detected and identified by gas chromatography-time-of-flight mass spectrometry (gc-tofms). the method was used to compare metabolite profiles of seven species of fungi grown on two types of sterile agars - potato dextrose and sabouraud dextrose. three species from the genus penicil ...200818625272
evaluation of messenger plant activator as a preharvest and postharvest treatment of sweet cherry fruit under a controlled atmosphere.the preservation methods as an alternative to chemical control to prevent postharvest quality losses of sweet cherry were examined. the efficacy of preharvest and postharvest messenger (m) treatments on sweet cherry cv. '0900 ziraat' was tested under a controlled atmosphere in 2004 and 2005. the factors investigated included the separate or combined effect of low oxygen, high carbon dioxide and m on the quality and fungal pathogens of sweet cherries in a normal atmosphere (na) and in a controlle ...200818671163
fungal transformation of dydrogesterone and inhibitory effect of its metabolites on the respiratory burst in human neutrophils.the biotransformation of the synthetic hormone dydrogesterone (1) by a number of fungal strains - including cephalosporium aphidicola, rhizopus stolonifer, cunninghamella elegans, and fusarium lini - afforded ten different metabolites, compounds 2-11. from a structural point of view, these transformations involved a combination of hydroxylation, oxidation, reduction, and/or epoxidation. the pregnane-based metabolites 10 and 11 are new compounds. all the known compounds were obtained for the firs ...200818293446
in vitro antifungal activity and mechanism of action of chitinase against four plant pathogenic fungi.to determine why chitinase has different antifungal activity on different pathogenic fungi in vitro, we purified recombinant rice chitinase from pichia pastoris and investigated its antifungal activity against four fungi - rhizopus stolonifer (ehrenb. et fr.) vuill, botrytis squamosa walker, pythium aphanidermatum (eds.) fitzp, and aspergillus niger van tiegh. scanning electron microscopy (sem) and fourier transform infrared spectroscopy (ftir) were used to analyze the surface microstructure and ...200818720488
microbial oxidation of anabolic steroids.microbial transformation of two anabolic steroids, ethylestrenol (1) and nandrolone (2), were carried out. ethylestrenol (1), when incubated with rhizopus stolonifer (tsy 0471), yielded two oxidative metabolites named 17alpha-ethyl-3beta,17beta-dihydroxy-19-norndrost-4-ene (3) and 17alpha-ethyl-17beta-hydroxy-19-norandrost-4-en-3-one (4), while incubation of compound 2 with the same fungus yielded two oxidative metabolites, 19-norandrost-4-en-3,17-dione (5) and 6alpha,17beta-dihydroxy-19-norandr ...200819023784
effect of some food preservatives on the lipolytic activity of beef luncheon fungi.beef luncheon meat is one of the most popular meals in several countries in the world including egypt. thirty one fungal species and 3 species varieties were recovered from 30 samples of beef luncheon meat collected from different supermarkets in qena. alternaria, aspergillus, emericella, mucor, mycosphaerella, penicillium and rhizopus were the most common genera on the two types of media. from the above genera, the most prevalent species were alternaria alternate, aspergillus flavus, a. fumigat ...200823997619
antibacterial and antifungal studies on some schiff base complexes of zinc(ii).two schiff base ligands l1and l2 were obtained by the condensation of glycylglycine respectively with imidazole-2-carboxaldehyde and indole-3-carboxaldehyde and their complexes with zn(ii) were prepared and characterized by microanalytical, conductivity measurement, ir, uv-vis., xrd and sem. the molar conductance measurement indicates that the zn(ii) complexes are 1: 1electrolytes. the ir data demonstrate the tetradentate binding of l1and tridentate binding of l2. the xrd data show that zn(ii) c ...200823990740
microbial transformation of testosterone by rhizopus stolonifer and fusarium lini.the microbial transformation of testosterone by the fungi rhizopus stolonifer and fusarium lini has been investigated for the first time. the bioconversion reactions observed from r. stolonifer included oxidation of a 17beta-hydroxyl group, a hydroxylation at equatorial 11alpha position, reduction of the double bond at c-4 with oxidation of the methylene at c-6 to the corresponding keto group, and lactonisation of ring d; the latter is the first report of this reaction by a rhizopus species. fus ...200819023814
electrochemical, antifungal, antibacterial and dna cleavage studies of some co(ii), ni(ii), cu(ii) and zn(ii)-copolymer complexes.cyclic voltammetric measurements were performed for co(ii), ni(ii), cu(ii) and zn(ii) complexes of 1: 1alternating copolymer, poly(3-nitrobenzylidene-1-naphthylamine-co-succinic anhydride) (l) and ni(ii) and cu(ii) complexes of 1: 1alternating copolymer, poly(3-nitrobenzylidene-1-naphthylamine-co-methacrylic acid) (l1). the in vitro biological screening effects of the investigated compounds were tested against the fungal species including aspergillus niger, rhizopus stolonifer, aspergillus flavu ...200823997637
antifungal effects of chitosan with different molecular weights on in vitro development of rhizopus stolonifer (ehrenb.:fr.) vuill.determination of the molecular weight of three types of chitosan was carried out by hpsec-ri. the effect of low, medium and high molecular weight chitosan was evaluated on development of three isolates of rhizopus stolonifer. image analysis and electronic microscopy observations were done in spores of this fungus. germination of r. stolonifer in potato dextrose broth with chitosan was also evaluated. results pointed out that the low molecular weight chitosan was more effective for inhibition of ...200826048219
enzyme activities and substrate degradation by fungal isolates on cassava waste during solid state fermentation.the growth and bioconversion potential of selected strains growing on cassava waste substrate during solid state fermentation were assessed. rhizopus stolonifer showed the highest and the fastest utilization of starch and cellulose in the cassava waste substrate. it showed 70% starch utilization and 81% cellulose utilization within eight days. the release of reducing sugars indicating the substrate saccharification or degradation potential of the organisms reached the highest value of 406.5 mg/g ...200724015097
variation of soil mycoflora in decomposition of rice stubble from rice-wheat cropping system.the colonization pattern and extent of decay produced in paddy stubble by soil inhabiting mycoflora were done by using nylon net bag technique. among the three methods used for isolation of fungi, dilution plate technique recorded the highest number of fungi followed by damp chamber and direct observation method. nutrient availability and climatic conditions (temperature, humidity and rainfall) influenced the occurrence and colonization pattern of fungi. maximum fungal population was recorded in ...200724015096
synthesis and spectral characterization of antifungal sensitive schiff base transition metal complexes.new n2o2 donor type schiff base has been designed and synthesized by condensing acetoacetanilido-4-aminoantipyrine with 2-aminobenzoic acid in ethanol. solid metal complexes of the schiff base with cu(ii), ni(ii), co(ii), mn(ii), zn(ii), vo(iv), hg(ii) and cd(ii) metal ions were synthesized and characterized by elemental analyses, magnetic susceptibility, molar conduction, fast atom bombardment (fab) mass, ir, uv-vis, and (1)h nmr spectral studies. the data show that the complexes have the compo ...200724015086
microbial transformation of the steroidal alkaloid dictyophlebine by rhizopus stolonifer.the microbial transformation of a steroidal alkaloid, dictyophlebine (1) with rhizopus stolonifer (atcc 10404) afforded three oxidized metabolites 2-4. compound 2 was found to be a new product. these metabolites were structurally characterized on the basis of modern spectroscopic techniques. their inhibitory activity towards acetyl- and butyrylcholinesterase has been evaluated and the new product 2 has been found to be more potent than the parent compound and other metabolites.200717409573
[cloning and expression of a delta6-fatty acid desaturase gene from rhizopus stolonifer in saccharomyces cervisiae].fatty acid composition of fungi is analysed through the gas chromatography( gc) technique. with specific activity a novel enzyme delta6-fatty acid desaturase was screened and isolated from rhizopus stolonifer. in this study r. stolonifer was identified as a fungal species that produced plentiful gamma-linolenic acid. a 1475bp full-length cdna, designated as rnd6d here, with high homology to fungal delta6-fatty acid desaturase genes was isolated from r. stolonifer using reverse transcription poly ...200717436625
suppression of phytopathogenic fungi by hexane extract of nepenthes ventricosa x maxima leaf.the hexane extract of nepenthes ventricosa x maxima leaf exhibited antifungal activity against alternaria alternata, aspergillus niger, bipolaris oryzae, fusarium oxysporum, phytophthora capsici, rhizoctonia solani, rhizopus stolonifer var. stolonifer and sclerotinia sclerotiorum.200717573203
the identification of antibacterial compounds for the development of enhanced media for the detection of foodborne fungi.in an effort to identify a more suitable antibiotic for utilization in mycological media, 12 food borne fungal species from various genera including alternaria alternata, aspergillus flavus, a. niger, eurotium chevalieri, fusarium moniliforme, penicillium sp., rhizopus stolonifer, saccharomyces cerevisiae, candida tropicalis, geotrichum candidum, rhodotorula glutinis and kluyveromyces thermotolerans along with 21 chloramphenicol-resistant bacterial isolates from fresh produce and atcc cultures o ...200717574697
steroid extraction in a microchannel system--mathematical modelling and experiments.the continuous ethyl acetate extraction of progesterone and 11alpha-hydroxyprogesterone, a reactant and the product of the biotransformation step involved in corticosteroid production, was studied in a microchannel at different flow velocities. in addition, non-steady state batch extraction without mixing was performed and modelled in order to verify the theoretically predicted parameters. in order to analyze experimental data and to forecast microreactor performance, a three-dimensional mathema ...200717594008
interactions between statins and penicillium chrysogenum antifungal protein (paf) to inhibit the germination of sporangiospores of different sensitive zygomycetes.this study reports on the antifungal activities of statins combined with an antifungal compound secreted by penicillium chrysogenum, paf. several species belonging in the class zygomycetes are considered to be agents of human or animal mycoses; other species have significance as postharvest plant pathogens. in the present work, four species (rhizopus stolonifer, mortierella wolfii, syncephalastrum racemosum and mycotypha africana) that exhibited different sensitivities to lovastatin and paf in p ...200717302920
association between sensitization to aureobasidium pullulans (pullularia sp) and severity of asthma.recent data indicate that fungi may contribute to increased severity of asthma.200717304882
polyphasic approach to the taxonomy of the rhizopus stolonifer group.phylogenetic analysis of rhizopus strains based on the d1/d2 region of lsu rdna sequences yielded a phylogram with four well-supported clades. the r. microsporus clade concurs with classification obtained by traditional methods. the r. oryzae group was found to include species of the genus amylomyces. the traditional r. stolonifer group was divided into two well-supported clades in the phylogram, with one clade comprising r. stolonifer var. stolonifer, r. sexualis var. sexualis, and r. sexualis ...200717324757
biosorption of anionic textile dyes by nonviable biomass of fungi and yeast.the nonviable biomass of aspergillus niger, aspergillus japonica, rhizopus nigricans, rhizopus arrhizus, and saccharomyces cerevisiae were screened for biosorption of textile dyes. the selected anionic reactive dyes were c.i. reactive black 8, c.i. reactive brown 9, c.i. reactive green 19, c.i. reactive blue 38, and c.i. reactive blue 3. experiments were conducted at initial dye concentration of 50, 100, 150 and 200mg/l. the effect of initial dye concentration, dose of biosorbent loading, temper ...200716997547
effect of yeast antagonist in combination with heat treatment on postharvest blue mold decay and rhizopus decay of peaches.the potential of using heat treatment alone or in combination with an antagonistic yeast for the control of blue mold decay and rhizopus decay of peaches caused by penicillium expansum and rhizopus stolonifer respectively, and in reducing natural decay development of peach fruits, as well as its effects on postharvest quality of fruit was investigated. in vitro tests, spore germination of pathogens in pdb was greatly controlled by the heat treatment of 37 degrees c for 2 d. in vivo test to contr ...200717140691
microbial and plant derived biomass for removal of heavy metals from wastewater.discharge of heavy metals from metal processing industries is known to have adverse effects on the environment. conventional treatment technologies for removal of heavy metals from aqueous solution are not economical and generate huge quantity of toxic chemical sludge. biosorption of heavy metals by metabolically inactive non-living biomass of microbial or plant origin is an innovative and alternative technology for removal of these pollutants from aqueous solution. due to unique chemical compos ...200716427277
synthesis of pseudopeptides based l-tryptophan as a potential antimicrobial agent.four compounds named l-btrppa, l-trp-o-pa, l-trp-m-pa and l-trp-p-pa, pseudopeptides constructed from pyridine and tryptophan units, were synthesized and tested against the gram-positive, gram-negative strains of bacteria and human pathogenic fungi. l-trp-o-pa proved to be a broad-spectrum antimicrobial agent, showing a significant inhibition of the growth of gram-positive bacteria (staphylococcus aureus, methicillin-resistant s. aureus, bacillus subtilis, micrococcus luteus), and pathogenic fun ...200717257839
mycoflora of two types of portuguese dry-smoked sausages and inhibitory effect of sodium benzoate, potassium sorbate, and methyl p-hydroxybenzoate on mold growth rate.the mycoflora of chouriqo types alentejano and ribatejano, two varieties of portuguese dry-smoked sausages, have been investigated after a producer-defined shelf life period (120 days at 20 +/- 5 degrees c) in modified atmosphere packaging (55% n2 and 45% co2). on the basis of morphological and physiological characteristics, the isolates were identified as penicillium, aspergillus, fusarium, rhizopus, monilia, absidia, and cephalosporium. the species identified were as follows: penicillium terre ...200717612078
bioremediation of aflatoxins by some reference fungal strains.aspergillus parasiticus rcmb 002001 (2) producing four types of aflatoxins b1, b2, g1, and g2 was used in this study as an aflatoxin-producer. penicillium griseofulvum, p. urticae, paecilomyces lilacinus, trichoderma viride, candida utilis, saccharomyces cerevisiae as well as a non-toxigenic strain of aspergillus flavus were found to be able to exhibit growth on aflatoxin b1-containing medium up to a concentration of 500 ppb. it was also found that several fungal strains exhibited the growth in ...200718062656
in vitro antioxidant and anti-rhizopus activities of lamiaceae herbal extracts.eighty-eight extracts of different polarity obtained from 18 lamiaceae medicinal and aromatic plants were screened for their antioxidant and antifungal properties. a tlc analysis combined with two bioassays were used to detect these activities. in most cases the highest radical scavenger capacity was detected in methanolic and aqueous extracts and polyphenols may be responsible. the most interesting antioxidant activity was observed in polar extracts obtained from lycopus europaeus, melissa offi ...200717912643
antifungal lactic acid bacteria with potential to prolong shelf-life of fresh vegetables.the aim of this study was to isolate and identify antifungal lactic acid bacteria from fresh vegetables, and evaluate their potential in preventing fungal spoilage of vegetables.200717850302
biotransformations of imbricatolic acid by aspergillus niger and rhizopus nigricans cultures.microbial transformation of imbricatolic acid (1) by aspergillus niger afforded 1alpha-hydroxyimbricatolic acid (2), while transformation with rhizopus nigricans yielded 15-hydroxy-8,17-epoxylabdan-19-oic acid (3). when the diterpene 1 was added to a cunninghamella echinulata culture, the main products were the microbial metabolites mycophenolic acid (4) and its 3-hydroxy derivative 5. all the structures were elucidated by spectroscopic methods. the cytotoxicity of these compounds towards human ...200717873843
microbial transformation of (-)-guaiol and antibacterial activity of its transformed products.microbial transformation of the sesquiterpene (-)-guaiol (1) [1(5)-guaien-11-ol] was investigated using three fungi, rhizopus stolonifer, cunninghamella elegans, and macrophomina phaseolina. fungal transformation of 1 with rhizopus stolonifer yielded a hydroxylated product, 1-guaiene-9 beta,11-diol (2). in turn, cunninghamella elegans afforded two mono- and dihydroxylated products, 1-guaiene-3beta,11-diol (3) and 1(5)-guaiene-3beta,9 alpha,11-triol (4), while macrophomina phaseolina produced two ...200717385913
composition and antifungal activity of essential oils of mentha piperita and lavendula angustifolia on post-harvest phytopathogens.the general antifungal activity of essential oils is well documented. the advantage of essential oils is their bioactivity in the vapor phase, a characteristic that makes them attractive as possible fumigants for stored product protection. essential oils of aerial parts of mentha piperita and lavendula angustifolia were obtained with hydrodistillation and oils composition identified with gc-ms. menthanol (36.24%) and menthone (32.42%) were the major compounds of the m. piperata essential oil. th ...200617390896
a comparative study on biosorption characteristics of certain fungi for bromophenol blue dye.laboratory investigations of the potential use of dried biomasses of rhizopus stolonifer, fusarium sp., geotrichum sp., and aspergillus fumigatus as biosorbents for the removal of bromophenol blue (bpb) dye from aqueous solutions were conducted. kinetics studies indicated that the bpb dye uptake processes can be well described by the pseudo-second-order model. the fungal biomasses exhibited the highest dye biosorption at ph 2.0. the langmuir adsorption model appears to fit the dye biosorption be ...200616891666
bioconversion of lignocellulose materials.one of the most economically viable processes for the bioconversion of many lignocellulosic waste is represented by white rot fungi. phanerochaete chrysosporium is one of the important commercially cultivated fungi which exhibit varying abilities to utilize different lignocellulosic as growth substrate. examination of the lignocellulolytic enzyme profiles of the two organisms phanerochaete chrysosporium and rhizopus stolonifer show this diversity to be reflected in qualitative variation in the m ...200624039492
biotransformation of physalin h and leishmanicidal activity of its transformed products.the transformation of physalin h (1) with rhizopus stolonifer and cunninghamella elegans has afforded two new physalins, 6,7-dehydrophysalin h (2) and 6-deoxyphysalin h (3), along with a known isophysalin b (4). their structures were elucidated by spectroscopic analysis. all of these compounds have shown potent leishmanicidal activity with ic50 values in the range of 6.03-13.80 microm.200616819205
the effect of storage time and agroecological zone on mould incidence and aflatoxin contamination of maize from traders in uganda.a study to determine mould incidence and aflatoxin contamination of maize kernels was carried out among dealers (traders) in the three agroecological zones of uganda. the maize kernels were categorized into those stored for two to six months or for more than six months to one year. results indicate that the mean moisture content of the kernels was within the recommended safe storage levels of < or =15% but was significantly lower in the highland maize kernels followed by the mid-altitude (dry) k ...200616822572
toxic micromycetes in grain raw material during its processing.in 2003-2005 micromycetes were isolated and identified from wheat, barley, rye, buckwheat grain brought into mills or from processing enterprises. contamination of the produced flour with micromycete propagules (cfu g(-1)), changes in micromycete diversity and abundance in the course of flour storage, preparation and baking of bread, production of groats or other food products and fodder were determined. most attention was given to widely distributed micromycetes, known producers of toxins: alte ...200616841886
exposure to airborne fungi during conservative dental treatment.the aim of the study was a mycological assessment of bioaerosol forming during conservative dental treatment, taking into account concentration and type of fungal microflora, and evaluation of the influence of duwl disinfecting protocol on the fungal contamination of the bioaerosol. the research was conducted on 25 operative sites located in public dental clinics. the air contained in the space between a patient and a dentist during conservative dental treatment with the use of a high-speed hand ...200616841889
biocatalytic and antimetastatic studies of the marine cembranoids sarcophine and 2-epi-16-deoxysarcophine.the soft coral sarcophyton glaucum is a rich resource of several bioactive cembranoids. sarcophytol a (1) and sarcophine (2) are cembranoid diterpenes reported from this soft coral and extensively investigated for their cancer chemopreventive properties. this study aimed at investigating the antimetastatic potential of the major cembranoids, sarcophine (2) and 2-epi-16-deoxysarcophine (3), from the red sea soft coral s. glaucum. biocatalytic transformation of 3 using rhizopus stolonifer atcc 622 ...200616872134
hydroxylation of the sesterterpene leucosceptrine by the fungus rhizopus stolonifer.the microbial transformation of leucosceptrine (1), the first member of class leucosesterterpenes, by rhizopus stolonifer afforded two metabolites, 1alpha-hydroxyleucosceptrine (2), and 8alpha-hydroxyleucosceptrine (3).200616426649
survey and significance of filamentous fungi from tap water.fungi in drinking water are involved in the production of tastes and odours in water. health problems are possible, originating from mycotoxins, animal pathogens and allergies. this report concerns the surveillance of mesophilic fungi in tap water and assessment of their potential for causing problems. the methods for the determination of the filamentous fungi (ff) were filtering, swabbing and baiting. tap water, half-strength corn meal, neopeptone-glucose rose bengal aureomycin (ngrba) and oomy ...200616459143
the microbial hydroxylation of levonorgestrel.the microbial transformation of levonorgestrel (1) by cunningham elegans resulted in the formation of five hydroxylated metabolites, 13-ethyl-10beta, 17beta-dihydroxy-18,19-dinor-17alpha-pregn-4-en-20-yn-3-one(2), 13-ethyl-6beta,17beta-dihydroxy-18,19-dinor-17alpha-pregn-4-en-20-yn-3-one (3) 13-ethyl 6beta, 10beta, 17beta-trihydroxy-18,19-dinor-17alpha-pregn-4-en-20-yn-3-one (4) 13-ethyl-15alpha-17beta-dihydroxy-18,19-dinor-17alpha-pregn-4-en-20-yn-3-one (5) and 13-ethyl-11alpha, 17beta-dihydrox ...200617201044
[moulds and yeasts in bottled water and soft drinks].some damaged cartons of soft drinks and carbonated water were analyzed to detect the microorganisms that caused the damage. the contaminants of sugar used in the production of one of the drinks were also studied. the methods of déak & beuchat and pitt & hocking were used for the identification of yeasts and moulds, respectively. the agents of the spoilage of soft drinks were debaryomyces hansenii, debaryomyces polymorphus, galactomyces geotrichum, metschnikowia pulcherrima, mucor circinelloides, ...200617037258
synthesis, structure and biological activity of cobalt(ii) and copper(ii) complexes of valine-derived schiff bases.we have synthesized two cobalt(ii) 2 and copper(ii) 3 complexes of valine-derived schiff bases. the obtained complexes were characterized by elemental analysis, ft-ir and x-ray diffraction. biological studies of complexes 2 and 3 had been carried out in vitro for antimicrobial activity against gram-positive, gram-negative bacteria and human pathogenic fungi. compound 3 was proven to be a broad spectrum agent, showed a significant inhibition of the growth of gram-positive bacteria (staphylococcus ...200616965817
characterization of some mushroom and earthy off-odors microbially induced by the development of rot on grapes.grape rot is one of the major causes of degradation of many grape components and, thus, of deterioration in wine quality. in particular, the association of botrytis cinerea with other, less visible, fungi frequently leads to the development of organoleptic defects in grapes and sometimes in wines. this study examines the nature of the volatile compounds responsible for mushroom, mossy, or earthy odors detected by gas chromatography-olfactometry in organic extracts of rotten grapes and musts. 2-m ...200617117809
structure elucidation and antibacterial activity of new fungal metabolites of sclareol.the transformation of the antibacterial diterpene sclareol (1) by two different fungal strains was investigated (scheme). in the presence of rhizopus stolonifer, (3beta)-3-hydroxysclareol (2), 18-hydroxysclareol (3), (6alpha)-6,18-dihydroxysclareol (4), and (11s)-11,18-dihydroxysclareol (5) were formed. fermentation of 1 with fusarium lini afforded (1beta)-1-hydroxysclareol (6) and (12s)-12-hydroxysclareol (7). compounds 4-7 were identified as new compounds, and some of them were active against ...200617193216
screening of cyanobacteria (blue-green algae) from rice paddy soil for antifungal activity against plant pathogenic fungi.soil cyanobacteria isolated from the rice paddy fields of 10 different locations across korea were evaluated by agar plate diffusion test for antifungal activity. aqueous, petroleum ether, and methanol extracts from one hundred and forty two cyanobacterial strains belonging to the 14 genera were examined for antifungal properties against seven phytopathogenic fungi causing diseases in hot pepper (capsicum annuum l). of total cyanobacteria, nine cyanobacteria (6.34%) exhibited antifungal effects. ...200624039487
raw starch degrading amylase production by various fungal cultures grown on cassava waste.the solid waste of sago industry using cassava was fermented by aspergillus niger, aspergillus terreus and rhizopus stolonifer in solid state fermentation. cassava waste contained 52 per cent starch and 2.9 per cent protein by dry weight. the amylase activity was maintained at a high level and the highest amylase activity was observed on the 8(th) day in r. stolonifer mediated fermentation. r. stolonifer was more efficient than aspergillus niger and aspergillus terreus in bioconverting cassava w ...200624039485
antifungal activities of biorelevant complexes of copper(ii) with biosensitive macrocyclic ligands.four copper(ii) complexes have been prepared using macrocyclic ligands. the macrocyclic ligands have been synthesized by the condensation reaction of diethyl phthalate with schiff bases derived from o-phenylene diamine and knoevenagel condensed β-ketoanilides (obtained by the condensation of acetoacetanilide and substituted benzaldehydes). the ligands and copper complexes have been characterized on the basis of microanalytical, mass, uv-vis., ir and cv spectral studies, as well as conductivity d ...200624039502
rhizopus soft rot on pear (pyrus serotina) caused by rhizopus stolonifer in korea.rhizopus soft rot caused by rhizopus stolonifer occurred on pears (pyrus serotina) in the jinju city agricultural products wholesale market in korea from 2004 to 2005. the infection usually started from wounds due to cracking at harvest time. the lesions started as water-soaked, rapidly softened, then gradually expanded. the mycelia grew vigorously on the surface of the fruits and formed stolons. colonies on potato dextrose agar at 25℃ were white cottony to brownish black. sporangia were globose ...200624039490
bioconversion of straw into improved fodder: fungal flora decomposing rice straw.the fungal flora decomposing rice straw were investigated all over the soil of sharkia province, east of nile delta, egypt, using the nylon net bag technique. sixty-four straw-decomposing species belonging to 30 genera were isolated by the dilution plate method in ground rice straw-czapek's agar medium at ph 6. the plates were incubated separately at 5℃, 25℃ and 45℃, respectively. twenty nine species belonging to 14 genera were isolated at 5℃. the most frequent genus was penicillium (seven speci ...200524049492
microbial hydroxylation of pregnenolone derivatives.pregnenolone and pregnenolone acetate were incubated with the fungi cunninghamella elegans, rhizopus stolonifer and gibberella fujikuroi. incubation of with c. elegans yielded metabolites, 3beta,7beta,11alpha-trihydroxypreg-5-en-20-one, 3beta,6alpha,11alpha,12beta,15beta-pentahydroxypreg-4-en-20-one and 3beta,6beta,11alpha-trihydroxypreg-4-en-20-one, while incubation with g. fujikuroi yielded two known metabolites, 3beta,7beta-dihydroxypregn-5-en-20-one and 6beta,15beta-dihydroxypreg-4-ene-3,20- ...200516272731
fungi, aflatoxins, fumonisin bl and zearalenone contaminating sorghum-based traditional malt, wort and beer in botswana.brewing and consumption of traditional beer have social-economic significance in most african countries including botswana. traditional sorghum malt, wort, and beer samples were collected from three villages around gaborone, botswana. forty-six malt samples were analyzed for fungi on three different media and developing colonies were subcultured for identification. rhizopus, fusarium, mucor, and aspergillus were the most common genera isolated. out of the 46 malt samples, 72% contained rhizopus ...200516170615
fungal metabolites of (e)-guggulsterone and their antibacterial and radical-scavenging activities.fungal transformation of (e)-guggulsterone (= (17e)-pregna-4,17-diene-3,16-dione; 1) by rhizopus stolonifer, fusarium lini, cunninghamella elegans, or gibberella fujikuroi afforded ten hydroxylated metabolites (2-11; scheme), which were fully characterized. compounds 4-11 have not been described yet. some of these novel hydroxylated metabolites, as well as acetylated derivatives thereof, exhibited significant antibacterial and radical-scavenging activities (table 3).200517192001
degradation of ochratoxin a and other mycotoxins by rhizopus isolates.several filamentous fungi representing the genera rhizopus and mucor were examined for their ability to degrade ochratoxin a (ota), aflatoxin b1, zearalenone and patulin in a liquid medium. while none of the isolates exhibited aflatoxin degrading activity, ochratoxin a, zearalenone and patulin were decomposed by several isolates. ochratoxin a was successfully degraded by rhizopus stolonifer, r. microsporus, r. homothallicus and two r. oryzae isolates, and by four unidentified rhizopus isolates. ...200515808366
in vitro susceptibility of 15 strains of zygomycetes to nine antifungal agents as determined by the nccls m38-a microdilution method.the in vitro susceptibility of 15 strains of six genera of zygomycetes including rhizopus oryzae (rhizopus arrhizus), rhizopus stolonifer, mucor circinelloides (three), absidia corymbifera (three), rhizomucor pusillus (three), cunninghamella bertholletiae (two), and syncephalastrum racemosum (two) to nine antifungal agents were determined by the nccls m38-a broth microdilution method. geometric means of the minimal inhibitory concentrations (mic) were: amphotericin b 0.07 mg l(-1) (range 0.03-0. ...200515982206
enzyme-catalyzed rearrangement of a diepoxy-germacrane compound into new 7-epi-eudesmane derivatives.two new 7-epi-eudesmane derivatives, together with two new germacrane compounds, have been isolated from the microbial-transformation of a (1alpha,10beta),(4beta,5alpha)-diepoxygermacrane using the hydroxylating fungi rhizopus nigricans. the rearranged skeleton and the stereochemistry of the chiral centers have been determined by means of their spectral data, and the absolute configuration has been confirmed by single-crystal x-ray analyses. a possible mechanism based on an enzyme-catalyzed isom ...200515624944
environmental mycological study and allergic respiratory disease among tobacco processing workers.this study presents the results of an investigation of respiratory symptoms, lung function, chest x-ray examinations, analysis of specific ige antibodies and skin prick tests to fungi on 130 tobacco-processing workers and 112 control workers. industrial hygiene survey and environmental mycological studies were also performed. the average dust concentrations ranged from 13.76 to 29.55 mg/m(3) in the tobacco processing workshops. also, the numbers of fungi colonies in the processing environments w ...200515824484
microbial transformation of prednisone.the microbial transformation of prednisone (17alpha,21-dihydroxy-pregna-1,4-diene-3,11,20-trione) (1) by cunninghamella elegans afforded two metabolites, 17alpha,21-dihydroxy-5alpha-pregn-1-ene-3,11,20-trione (2) and 17alpha,20s,21-trihydroxy-5alpha-pregn-1-ene-3,11-dione (3), while the fermentation of 1 with fusarium lini, rhizopus stolonifer and curvularia lunata afforded a metabolite 1,4-pregnadiene-17alpha,20s,21-triol-3,11-dione (4). compound 3 was found to be a new metabolite. their struct ...200515938135
[in vitro susceptibility of fungal and yeast clinical isolates to itraconazole and voriconazole].the interest on the in vitro susceptibility to itraconazole has recently increased due the availability of the intravenous formulation. in this study, comparative mics of this antifungal with voriconazole were carried out in 62 clinical isolates of filamentous fungi and 100 yeasts isolates using the nccls microbroth methods described in m38-a and m27-a2 documents. a mic90 of 0.125 micrograms per ml was observed for itraconazole and voriconazole against aspergillus fumigatus. higher susceptibilit ...200516107169
the involvement of camp in the growth inhibition of filamentous fungus rhizopus nigricans by steroids.several steroids, in particular progesterone, are toxic for the filamentous fungus rhizopus nigricans and, at high concentrations, inhibit its growth. previous studies on this microorganism revealed progesterone specific receptors coupled to g proteins at the plasma membrane. in this study, the next step of steroid signalling in r. nigricans following g protein activation is investigated, together with the possible impact of this pathway on fungal growth inhibition. the intracellular level of ca ...200515621431
continuous adsorption and recovery of cr(vi) in different types of reactors.this study reports the results of experiments on continuous adsorption and desorption of cr(vi) ions by a chemically modified and polysulfone-immobilized biomass of the fungus rhizopus nigricans. a fixed quantity of polymer-entrapped biomass beads corresponding to 2 g of dry biomass powder was employed in packed bed, fluidized bed, and stirred tank reactor for monitoring the continuous removal and recovery of cr(vi) ions from aqueous solution and synthetic chrome plating effluent. parameters suc ...200516321053
microbial hydroxylation of sclareol by rhizopus stolonifer.incubation of sclareol with rhizopus stolonifer affords in high yield a mixture of triols with 18-hydroxy-sclareol as the main component.200518007369
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