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in vivo evaluation of skin irritation potential, melasma and sebum content following long term application of skin care cream in healthy adults, using non-invasive biometrological techniques.the present investigation was conducted to evaluate non-invasively, various functional skin parameters i.e., irritation potential, melasma and sebum contents following long term application of topical cream (w/o) loaded with 2% methanolic extract of ananas comosus l. versus placebo control (base) in healthy adults. healthy human volunteers (n = 11, aged 20-30 years) were recruited for investigation and written informed consent was taken from each volunteer. in this single blinded study every vol ...201627008816
physicochemical, functional and antioxidant properties of tropical fruits co-products.the aim of this study was to determine the physicochemical, functional and antioxidant properties of mango (mac), pineapple (pac) and passion fruit (pfc) co-products in order to evaluate them as ingredients for food application. proximate composition showed low fat content (0.95-5.64 g/100 g), and high levels of dietary fiber. in pineapple and passion fruit co-products, dietary fiber represented more than 50 % of the sample. low ph, water activity, along with high acidity indicated that these co ...201626984339
de novo transcriptome assembly of floral buds of pineapple and identification of differentially expressed genes in response to ethephon induction.a remarkable characteristic of pineapple is its ability to undergo floral induction in response to external ethylene stimulation. however, little information is available regarding the molecular mechanism underlying this process. in this study, the differentially expressed genes (degs) in plants exposed to 1.80 ml·l(-1) (t1) or 2.40 ml·l(-1) ethephon (t2) compared with ct plants (control, cleaning water) were identified using rna-seq and gene expression profiling. illumina sequencing generated 6 ...201626955375
comparative study of antitumor effects of bromelain and papain in human cholangiocarcinoma cell lines.cholangiocarcinoma (cc) worldwide is the most common biliary malignancy with poor prognostic value and new systemic treatments are desirable. plant extracts like bromelain and papain, which are cysteine proteases from the fruit pineapple and papaya, are known to have antitumor activities. therefore, in this study for the first time we investigated the anticancer effect of bromelain and papain in intra- and extrahepatic human cc cell lines. the effect of bromelain and papain on human cc cell grow ...201626935541
odors identification differences in deficit and nondeficit schizophrenia.there is evidence that deficit schizophrenia (ds) is associated with neuroanatomical changes in structures including those involved in olfaction. olfactory dysfunction, which includes impaired odor identification, is found in patients with schizophrenia and their family members.201626922544
pineapple genome: a reference for monocots and cam photosynthesis.pineapple occupies an important phylogenetic position and its reference genome expedites genomic research within the family bromeliaceae and more widely among monocots. one such research focus is the evolution of crassulacean acid metabolism (cam) photosynthesis. acquiring circadian clock cis-regulatory elements in cam-related genes might be a critical step in the evolution of this form of photosynthesis. follow-up studies will clarify the processes and evolutionary forces leading to the multipl ...201627613080
in situ toxicity and ecological risk assessment of agro-pesticide runoff in the madre de dios river in costa rica.the river madre de dios (rmd) and its lagoon is a biodiversity rich watershed formed by a system of streams, rivers, channels, and a coastal lagoon communicating with the caribbean sea. this basin sustains a large area of agricultural activity (mostly banana, rice, and pineapple) with intensive use of pesticides, continually detected in water samples. we investigated in situ the toxicological effects caused by pesticide runoff from agriculture and the relation of pesticide concentrations with di ...201627757743
comparative genomics analysis of rice and pineapple contributes to understand the chromosome number reduction and genomic changes in grasses.rice is one of the most researched model plant, and has a genome structure most resembling that of the grass common ancestor after a grass common tetraploidization ∼100 million years ago. there has been a standing controversy whether there had been five or seven basic chromosomes, before the tetraploidization, which were tackled but could not be well solved for the lacking of a sequenced and assembled outgroup plant to have a conservative genome structure. recently, the availability of pineapple ...201627757123
real-time mri of swallowing: intraoral pressure reduction supports larynx elevation.the reduction in intraoral pressure during swallowing has previously been linked to bolus transport, although no such relation has yet been proven. the purpose of this work was to evaluate the time course of intraoral pressure during swallowing using simultaneous real-time magnetic resonance imaging (mri) and dynamic pressure recordings. real-time mri based on highly undersampled radial fast low-angle shot (flash) and regularized nonlinear inverse reconstruction was performed at 3 t using a stan ...201627687293
bromelain has paradoxical effects on blood coagulability: a study using thromboelastography.bromelain is a crude extract from pineapple that is known for a wide array of pharmacological effects including protein digestion, fibrinolytic and anti-immune inflammatory effects and has been popularly used as a phytotherapeutic drug. however, its clinical values and applications remain understudied. the aim of this study was to investigate the effect of bromelain on the coagulability of blood using thromboelastography (teg). we identified 0.4 u/ml as the minimum concentration of bromelain tha ...201625517253
papaya latex supernatant has a potent effect on the free-living stages of equid cyathostomins in vitro.the control of equid gastrointestinal nematodes in developed countries, in particular the cyathostomins, is threatened by high levels of anthelmintic resistance. in recent years, there has been increasing interest in the evaluation of traditional 'ethnoveterinary' medicines as alternatives to chemical anthelmintics. the cysteine proteinases (cps), a group of enzymes derived from fruits such as papaya (carica papaya), pineapple (ananas comosus) and figs (ficus spp.), have shown good efficacy agai ...201627692327
principal component analysis (pca) of volatile terpene compounds dataset emitted by genetically modified sweet orange fruits and juices in which a d-limonene synthase was either up- or down-regulated vs. empty vector controls.we have categorized the dataset from content and emission of terpene volatiles of peel and juice in both navelina and pineapple sweet orange cultivars in which d-limonene was either up- (s), down-regulated (as) or non-altered (ev; control) ("impact of d-limonene synthase up- or down-regulation on sweet orange fruit and juice odor perception"(a. rodríguez, j.e. peris, a. redondo, t. shimada, e. costell, i. carbonell, c. rojas, l. peña, (2016)) [1]). data from volatile identification and quantific ...201627689126
combination of cisplatin and bromelain exerts synergistic cytotoxic effects against breast cancer cell line mda-mb-231 in vitro.bromelain, which is a cysteine endopeptidase commonly found in pineapple stems, has been investigated as a potential anti-cancer agent for the treatment of breast cancer. however, information pertaining to the effects of combining bromelain with existing chemotherapeutic drugs remains scarce. this study aimed to investigate the possible synergistic cytotoxic effects of using bromelain in combination with cisplatin on mda-mb-231 human breast cancer cells.201627891174
mechanism of internal browning of pineapple: the role of gibberellins catabolism gene (acga2ox) and gas.internal browning (ib), a physiological disorder (pd) that causes severe losses in harvested pineapple, can be induced by exogenous gibberellins (gas). over the years, studies have focused on roles of gibberellin 2-oxidase (ga2oxs), the major gas catabolic enzyme in plants, in the regulation of changes in morphology or biomass. however, whether ga2oxs could regulate pd has not been reported. here, a full-length acga2ox cdna was isolated from pineapple, with the putative protein sharing 23.59% to ...201627982026
evaluation of bioactive compounds, chemical and technological properties of fruits byproducts powder.millions of tons of fruit byproducts are discarded globally every day by food processing industries, which represents a considerable loss in terms of nutrients. the goal of this work was to evaluate the physico-chemical, technological, antioxidant properties and characterization of carotenoids for papaya, pineapple, olive byproducts and anthocyanins for blueberry byproducts. the results indicated that these byproducts are good sources of total dietary fiber, especially olive byproducts (53.68%). ...201628035162
local cell death changes the orientation of cell division in the developing drosophila wing imaginal disc without using fat or dachsous as orienting signals.drosophila imaginal disc cells exhibit preferred cell division orientations according to location within the disc. these orientations are altered if cell death occurs within the epithelium, such as is caused by cell competition or by genotypes affecting cell survival. both normal cell division orientations, and their orientations after cell death, depend on the fat-dachsous pathway of planar cell polarity (pcp). the hypothesis that cell death initiates a planar polarity signal was investigated. ...201628030539
nutritional beliefs and practices among diabetic pregnant mothers in a tertiary care hospital in bangladesh.pregnant mothers with diabetes, especially in developing countries, are particularly prone to suffer from dietary imbalances due to superstitions and irrational beliefs and practices. the study aimed to explore the existing beliefs and practices related to food intake among bangladeshi diabetic pregnant mothers.201628017280
diet intake patterns of non-bengali muslim mothers during pregnancy and lactation.an explorative study was carried out to ascertain the knowledge, attitudes, and practices regarding diet patterns during pregnancy and lactation among non-bengali muslim mothers. 100 consecutive mothers admitted for delivery in the obstetric ward of islamia hospital, calcutta, were selected as index cases. retrospective data from these mothers were collected about their diet patterns during pregnancy. at least 3 home visits were made for each mother after her discharge from the hospital: the 1st ...20162641755
pineelm_ssrdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and est sequences of pineapple (ananas comosus (l.) merrill).simple sequence repeats or microsatellites are resourceful molecular genetic markers. there are only few reports of ssr identification and development in pineapple. complete genome sequence of pineapple available in the public domain can be used to develop numerous novel ssrs. therefore, an attempt was made to identify ssrs from genomic, chloroplast, mitochondrial and est sequences of pineapple which will help in deciphering genetic makeup of its germplasm resources.201628096778
novel approach of using nutraceutic-directed caloric antioxidant density and ion-ratio for evaluating fruit's health quality.seven kinds of indigenous fruits and five imported fruits were compared for their "health quality." methods including the calorific, antioxidant, and ion ratios were carried out. results indicated the order of content (in mg/100g) was: ca(2+) , murcott orange (218.2) > kiwifruit (200.0) > pineapple (138.5) > golden kiwi (117.6); mg(2+) , pitaya (192.2), banana (88.0), kiwifruit (63.4), and golden kiwi (58.4); zn(2+) , pitaya (19.53) > pear (10.8) > kiwifruit (6.09) > irwin mango (4.58). cu(2+) , ...201627467811
comparative analysis on the distribution of protease activities among fruits and vegetable resources.in this study, a comparative analysis on the distribution of protease activities among 90 plant resources, including fruits and vegetables, has been performed. protease activities of plant extracts were assayed at different ph values (ph 3.0, ph 7.5 and ph 10.5) using casein as a substrate. ten fruits and thirteen vegetables show protease activities above 10u/g. pineapple, fig and papaya, which are used for commercial protease production, exhibited high protease activities. additionally, high pr ...201627451238
optimization of pineapple pulp residue hydrolysis for lipid production by rhodotorula glutinis tistr5159 using as biodiesel feedstock.the higher lipid productivity of rhodotorula glutinis tistr5159 was achieved by optimizing the pineapple pulp hydrolysis for releasing the high sugars content. the sequential simplex method operated by varied; solid-to-liquid ratio, sulfuric acid concentration, temperature, and hydrolysis time were successfully applied and the highest sugar content (83.2 g/l) evaluated at a solid-to-liquid ratio of 1:10.8, 3.2% sulfuric acid, 105 °c for 13.9 min. moreover, the (nh4)2so4 supplement enhanced the l ...201627149319
table wine from tropical fruits utilizing natural yeast isolates.an attempt was made to utilize few widely available tropical fruits to develop wine with the objective of comparing the fermentation efficiency (along with progress in fermentation) of two efficient yeast isolates with commercially available strain. fruit wine from juices of fully ripe mango, jackfruit and pineapple alone and in blended combinations of all three fruit juice (2: 1: 2) was prepared using two different yeasts (y4 and y7) isolated from natural plain date palm juice and one standard ...201627570291
identification, stress tolerance, and antioxidant activity of lactic acid bacteria isolated from tropically grown fruits and leaves.from 6 samples of tropically grown fruits and leaves, 10 lactic acid bacteria belonging leuconostoc, weissella, and lactobacillus species were isolated and identified by 16s rrna gene sequencing and (gtg)5 fingerprinting. acidification kinetics determined from bhi broth cultures showed genus-related patterns. in particular, weissella cibaria appeared to act as a potent acidifier. tolerance of isolates to acid, oxidative, or salt stress was highly variable and strain dependent. isolate s14 (leuco ...201627197991
transcriptional reprogramming and phenotypic switching associated with the adaptation of lactobacillus plantarum c2 to plant niches.lactobacillus plantarum has been isolated from a large variety of ecological niches, thus highlighting its remarkable environmental adaptability as a generalist. plant fermentation conditions markedly affect the functional features of l. plantarum strains. we investigated the plant niche-specific traits of l. plantarum through whole-transcriptome and phenotypic microarray profiles. carrot (cj) and pineapple (pj) juices were chosen as model systems, and mrs broth was used as a control. a set of 3 ...201627273017
characteristics of lactic acid bacteria isolates and their effect on silage fermentation of fruit residues.the natural lactic acid bacteria (lab) population, chemical composition, and silage fermentation of fruit residues were studied. eighty-two strains of lab were isolated from fruit residues such as banana leaf and stem, pineapple peel, and papaya peel. all strains were gram-positive and catalase-negative bacteria, and they were divided into 7 groups (a-g) according to morphological and biochemical characters. strains in groups a to f were rods, and group g was cocci. group f produced gas from glu ...201627108171
the efficacy of mentha arvensis l. and m. piperita l. essential oils in reducing pathogenic bacteria and maintaining quality characteristics in cashew, guava, mango, and pineapple juices.this study evaluated the ability of the essential oil from mentha arvensis l. (maeo) and m. piperita l. (mpeo) to induce ≥5-log reductions in counts (cfu/ml) of e. coli, l. monocytogenes, and salmonella enterica serovar enteritidis in brain-heart infusion broth (bhib) and cashew, guava, mango, and pineapple juices during refrigerated storage (4±0.5°c). the effects of the incorporation of these essential oils on some physicochemical and sensory parameters of juices were also evaluated. the incorp ...201627648760
survival and expression of acid resistance genes in shiga toxin-producing escherichia coli acid adapted in pineapple juice and exposed to synthetic gastric fluid.the aim of this research was to determine the ability of non-o157 shiga toxin-producing escherichia coli (stec) serogroups to survive with exposure to synthetic gastric fluid (sgf) after adaptation to pineapple juice (pj) at room and refrigerated temperatures compared to e. coli o157:h7 and to examine the relative transcriptional expression of acid resistance (ar) genes, rpos, gada and adia.201627377411
antibacterial and antiproliferative peptides in synbiotic yogurt-release and stability during refrigerated storage.the search for alternative therapeutics is on the rise due to the extensive increase in bacterial resistance to various conventional antibiotics and side effects of conventional cancer therapies. bioactive peptides released from natural sources such as dairy foods by lactic acid bacteria have received attention as a potential source of biotherapeutic peptides. however, liberation of peptides in yogurt depends on proteolytic activities of the cultures used. thus, this research was conducted to es ...201626995128
fusarium proliferatum strains change fumonisin biosynthesis and accumulation when exposed to host plant extracts.fumonisin concentrations in mycelia and media were studied in liquid fusarium proliferatum cultures supplemented with host plant extracts. furthermore, the kinetics of fumonisin accumulation in media and mycelia collected before and after extract addition was analysed as well as the changes in the expression of the fum1 gene. fumonisin content in culture media increased in almost all f. proliferatum strains shortly after plant extracts were added. the asparagus extract induced the highest fb lev ...201627268248
inactivation of escherichia coli, listeria monocytogenes, and salmonella enteritidis by cymbopogon citratus d.c. stapf. essential oil in pineapple juice.in the present study, the efficacy of cymbopogon citratus d.c. stapf. essential oil (cceo) to provoke a 5-log cfu/ml (5-log) inactivation in a mixed composite of escherichia coli, listeria monocytogenes, and salmonella enterica serovar enteritidis in pineapple (ananas comosus (l.) merril) juice (4°c) was assessed. moreover, the effects of cceo on the physicochemical and sensory quality parameters of pineapple juice were evaluated. the mic of cceo was 5 μl/ml against the composite mix examined. f ...201626818981
current status of tropical fruit breeding and genetics for three tropical fruit species cultivated in japan: pineapple, mango, and papaya.tropical fruit crops are predominantly produced in tropical and subtropical developing countries, but some are now grown in southern japan. pineapple (ananas comosus), mango (mangifera indica) and papaya (carica papaya) are major tropical fruits cultivated in japan. modern, well-organized breeding systems have not yet been developed for most tropical fruit species. most parts of japan are in the temperate climate zone, but some southern areas such as the ryukyu islands, which stretch from kyushu ...201627069392
plant protease inhibitors in therapeutics-focus on cancer therapy.plants are known to have many secondary metabolites and phytochemical compounds which are highly explored at biochemical and molecular genetics level and exploited enormously in the human health care sector. however, there are other less explored small molecular weight proteins, which inhibit proteases/proteinases. plants are good sources of protease inhibitors (pis) which protect them against diseases, insects, pests, and herbivores. in the past, proteinaceous pis were considered primarily as p ...201628008315
polymer-based alternative method to extract bromelain from pineapple peel waste.bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (ananas comosus merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. the use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. we aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two-ph ...201624011234
aroma profile and volatiles odor activity along gold cultivar pineapple flesh.physicochemical attributes, aroma profile, and odor contribution of pineapple flesh were studied for the top, middle, and bottom cross-sections cut along the central axis of gold cultivar pineapple. relationships between volatile and nonvolatile compounds were also studied. aroma profile constituents were determined by headspace solid-phase microextraction at 30 °c, followed by gas chromatography/mass spectrometry analysis. a total of 20 volatile compounds were identified and quantified. among t ...201621535624
intra-oral acid production associated with eating whole or pulped raw fruits.the hypotheses that raw fruits, whether whole or pulped, were cleared rapidly from the mouth and that the sugars in the whole and pulped fruits are fermented with equal efficiency to acids by the oral microflora were tested in this study. groups of 7-9 adult subjects chewed 10 g of raw, whole or pulped fruit (apple, banana, orange, pear and pineapple) for 1 min and whole, unstimulated saliva samples were collected during the following 60-min interval. each saliva sample was assayed for the conce ...201615181333
normative data for commercial pineapple juice from concentrate.normative data for pineapple juice from concentrate were determined for 19 samples, including 5 that had been aseptically processed and representing 4 of the major pineapple growing regions of the world. values are reported for sugars, organic acids, including isocitric acid, metals (specifically potassium, sodium calcium, and magnesium), delta 13c, and oligosaccharides. although geographical variation existed, the observed ranges and variances were small enough to be useful in describing authen ...20168069130
the precipitation of symptoms by common foods in children with atopic dermatitis.atopic dermatitis (ad) is a chronic and disabling condition that has a major impact on financial and social resources of the individual and the community. its incidence is increasing dramatically, and no cure is available. pharmacological treatment is only partially effective. the evidence that diet plays a role in children with atopic dermatitis is now irrefutable. prophylactic measures can prevent or limit the development of ad, and partially restricted diets can modify the disease's course or ...20167806078
occupational exposure to 1,3-dichloropropene (telone ii) in hawaiian pineapple culture.1,3-dichloropropene (dcp), the primary constituent of telone ii, is a subsoil fumigant that has supplanted 1,2-dibromo-3-chloropropane (dbcp) and ethylene dibromide (edb) as primary fumigant in hawaiian pineapple culture. to determine the potential for adverse health effects, an environmental survey was done to assess worker exposures. exposures were predominantly below 1 ppm, which is the no-effect level determined with experimental animals, and the threshold limit value (tlv) recommended by th ...20163452296
individual variation of the fruits of the pineapple (ananas comosus l. merr.) in regard to certain constituents of the juice. 201614840519
studies on vitamin content of canned pineapple. 201621025058
[treatment of cervical erosions with siberian pineapple oil]. 201613282819
azoospermia and oligospermia among a large cohort of dbcp applicators in 12 countries.azoospermia and oligospermia have been well demonstrated among workers exposed to 1,2-dibromo-3-chloropropane (dbcp) in manufacturing and formulation of this pesticide. after dbcp was banned in the united states in the late 1970s, two american companies continued to export it to many less developed countries. in the early to mid-1990s, attorneys assembled a cohort of approximately 26,400 male plaintiffs who, as workers on banana and pineapple plantations in 12 of these countries, had been expose ...201510330512
antioxidant capacity of infant fruit beverages: influence of storage and in vitro gastrointestinal digestion.the total antioxidant capacity of three beverages based on fruit juice, milk and cereals, intended for infants and young children up to 3 years of age was evaluated by two methods trolox equivalent antioxidant capacity and oxygen radical absorption capacity.201519132262
bromelain limits airway inflammation in an ovalbumin-induced murine model of established asthma.allergic asthma continues to increase despite new pharmacological advances for both acute treatment and chronic-disease management. asthma is a multifactorial disease process with genetic, allergic, infectious, environmental, and dietary origins. researchers are investigating the benefits of lifestyle changes and alternative asthma treatments, including the ability of bromelain to inhibit inflammation. bromelain is a commonly used, proteolytically active pineapple extract.201522894886
palatability, digestibility and emotional pattern in 60 healthy volunteers after ingestion of an iced dessert presented in four different flavours: a subjective evaluation.several variables lead to changes in human and animal eating behaviour and food choices. a pivotal role is played by food palatability, represented by food, smell, taste, texture, appearance and temperature. the aim of our study is to assess the potential differences in palatability and digestibility of four different flavoured iced desserts, consumed at the end of a standardized meal, and their impact on the emotional status of 60 healthy volunteers. sixty healthy volunteers, after ent and psyc ...201522824751
occurrence of cellulose-producing gluconacetobacter spp. in fruit samples and kombucha tea, and production of the biopolymer.cellulose producing bacteria were isolated from fruit samples and kombucha tea (a fermented beverage) using cuso4 solution in modified watanabe and yamanaka medium to inhibit yeasts and molds. six bacterial strains showing cellulose production were isolated and identified by 16s rrna gene sequencing as gluconacetobacter xylinus strain dfbt, ga. xylinus strain dfr-1, gluconobacter oxydans strain dfr-2, g. oxydans strain dfr-3, acetobacter orientalis strain dfr-4, and gluconacetobacter intermedius ...201525926011
applications of microencapsulated bifidobacterium longum with eleutherine americana in fresh milk tofu and pineapple juice.bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. microencapsulation of b. longum with eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. microencapsulated b. longum with the extract and oligosaccharides extract in the food products showed better sur ...201525854832
fungal presence in selected tree nuts and dried fruits.sixty-four tree nut samples (almonds, pecans, pine nuts, and walnuts) and 50 dried fruit samples (apricots, cranberries, papaya, pineapple, and raisins) were purchased from local supermarkets and analyzed for fungal contamination using conventional culture as well as molecular methods. the results of our study showed that the highest yeast and mold (ym) counts (5.34 log10 cfu g(-1)) were found in walnuts and the lowest in pecans. the most common mold in nuts was aspergillus niger, relatively low ...201526056470
isolation and characterization of bacteriocinogenic lactic bacteria from m-tuba and tepache, two traditional fermented beverages in méxico.mexican tuba (m-tuba) and tepache are mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. at present, reports on the microbiota and nutritional effects of both beverages are lacking. the purpose of this study was to determine whether m-tuba and tepache contain cultivable lactic acid bacteria (lab) capable of producing bacteriocins. tepache and m-tuba contain mesophilic aerobic bacteria, lab, and yeast. bacillus subtilis, listeria monocytogenes, listeri ...201526405529
molecular cloning and characterization of udp-glucose: furaneol glucosyltransferase gene from grapevine cultivar muscat bailey a (vitis labrusca × v. vinifera).2,5-dimethyl-4-hydroxy-3(2h)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from american grape (vitis labrusca) and its hybrid grape. furaneol glucoside was recently isolated as an important furaneol derivative from the hybrid grapevine cultivar, muscat b ...201526160581
dai nippon printing co., ltd medi·ca cc for enumeration of coliform bacteria.a ready-made dry medium method for coliform count, the medi·ca cc method, was compared to the violet red bile agar method (bacteriological analytical manual, chapter 4, enumeration of escherichia coli and the coliform bacteria, section g) for nine raw foods from four food categories: raw ground pork, raw lamb, raw ground chicken, raw tuna fillet, raw salmon fillet, raw shrimp, fresh peeled banana, fresh cut pineapple, and fresh cut apple. the 95% confidence interval for the mean difference betwe ...201525857880
host extract modulates metabolism and fumonisin biosynthesis by the plant-pathogenic fungus fusarium proliferatum.fusarium proliferatum is a common pathogen able to infect a broad range of agriculturally important crops. recently, some evidence for genetic variance among the species genotypes in relation to their plant origin has been reported. mycotoxin contamination of plant tissues is the most important threat caused by f. proliferatum and fumonisins b (fbs) are the principal mycotoxins synthesized. the toxigenic potential of the pathogen genotypes is variable and also the reaction of different host plan ...201525462926
phenylpropanoid glycosides from the leaves of ananas comosus.two new phenylpropanoid glycosides, named β-d-(1-o-acetyl-3,6-o-diferuloyl) fructofuranosyl β-d-6'-o-acetylglucopyranoside (1) and β-d-(1-o-acetyl-3,6-o-diferuloyl) fructofuranosyl α-d-glucopyranoside (2), along with two known analogues (3-4) and four glycerides (5-8), were isolated from the etoac extract of the leaves of ananas comosus. their structures were elucidated on the basis of 1d- and 2d-nmr analyses, as well as hr-esi-ms experiments. compounds 1-4 showed significant antibacterial activ ...201526882684
growth potential of listeria monocytogenes and staphylococcus aureus on fresh-cut tropical fruits.the objective of this study was to evaluate the fate of staphylococcus aureus, listeria monocytogenes, and natural microbiota on fresh-cut tropical fruits (pitaya, mango, papaya and pineapple) with commercial pvc film at different storage temperature (5, 13, and 25 °c). the results showed that s. aureus, l. monocytogenes, and natural microbiota increased significantly on fresh-cut tropical fruits at 25 °c. both pathogen and natural microbiota were able to grow on fresh-cut tropical fruits at 13 ...201526862605
phenotypic and molecular diversity of meyerozyma guilliermondii strains isolated from food and other environmental niches, hints for an incipient speciation.meyerozyma guilliermondii is a yeast species widely isolated from several natural environments and from fruit; in medical microbiology it is known as the teleomorph of the opportunistic pathogen candida guilliermondii, which causes about 2% of the human blood infections. this yeast is also promising in a variety of biotechnological applications as vitamins production and post-harvest control. the question if isolates from different sources are physiologically and genetically similar, or if the v ...201525791010
effect of refrigerated storage on probiotic viability and the production and stability of antimutagenic and antioxidant peptides in yogurt supplemented with pineapple peel.fruit by-products are good resources of carbohydrates, proteins, vitamins, and minerals, which may function as growth nutrients for probiotic bacteria. this research aimed at evaluating effects of pineapple peel powder addition on the viability and activity of lactobacillus acidophilus (atcc 4356), lactobacillus casei (atcc393), and lactobacillus paracasei ssp. paracasei (atcc baa52) in yogurts throughout storage at 4°c for 28d. plain and probiotic yogurts supplemented with or without pineapple ...201526142843
pantoea ananatis utilizes a type vi secretion system for pathogenesis and bacterial competition.type vi secretion systems (t6sss) are a class of macromolecular machines that are recognized as an important virulence mechanism in several gram-negative bacteria. the genome of pantoea ananatis lmg 2665(t), a pathogen of pineapple fruit and onion plants, carries two gene clusters whose predicted products have homology with t6ss-associated gene products from other bacteria. nothing is known regarding the role of these t6ss-1 and t6ss-3 gene clusters in the biology of p. ananatis. here, we presen ...201525411959
analysis of pineapple mealybug wilt associated virus -1 and -2 for potential rna silencing suppressors and pathogenicity factors.higher plants use rna silencing to defend against viral infections. as a counter defense, plant viruses have evolved proteins that suppress rna silencing. mealybug wilt of pineapple (mwp), an important disease of pineapple, has been associated with at least three distinct viruses, pineapple mealybug wilt associated virus -1, -2, and -3 (pmwav-1, -2, and -3). selected open reading frames (orfs) of pmwav-1 and pmwav-2 were screened for their local and systemic suppressor activities in agrobacteriu ...201525751306
application of membrane separation in fruit and vegetable juice processing: a review.fruit and vegetable juices are used due to convenience. the juices are rich in various minerals, vitamins, and other nutrients. to process the juices and their clarification and/or concentration is required. the membranes are being used for these purposes. these processes are preferred over others because of high efficiency and low temperature. membranes and their characteristics have been discussed in brief for knowing suitability of membranes for fruit and vegetable juices. membrane separation ...201524915352
dengue viruses in aedes albopictus skuse from a pineapple plantation in costa rica. 201526047200
the relative anthelmintic efficacy of plant-derived cysteine proteinases on intestinal nematodes.we examined the in vitro and in vivo efficacy of plant cysteine proteinases (cps) derived from pineapple (ananas comosus) and kiwi fruit (actinidia deliciosa), and compared their efficacy as anthelmintics to the known effects of cps from the latex of papaya (carica papaya) against the rodent intestinal nematode, heligmosomoides bakeri. both fruit bromelain and stem bromelain had significant in vitro detrimental effects on h. bakeri but in comparison, actinidain from kiwi fruit had very little ef ...201524176056
agave as a model cam crop system for a warming and drying world.as climate change leads to drier and warmer conditions in semi-arid regions, growing resource-intensive c3 and c4 crops will become more challenging. such crops will be subjected to increased frequency and intensity of drought and heat stress. however, agaves, even more than pineapple (ananas comosus) and prickly pear (opuntia ficus-indica and related species), typify highly productive plants that will respond favorably to global warming, both in natural and cultivated settings. with nearly 200 ...201526442005
[detection of syrup adulterants in prepackaged pure pineapple juice by fourier-transform infrared spectroscopy and chemometric analysis].this study was performed to establish a method that can quickly and accurately identify adulterated syrup in the pure pineapple juice. a attenuated total internal refraction-fourier transform infrared spectroscopy was used to collect the range of 900 -1 500 cm(-1) infrared spectra of 234 samples pure pineapple juice and adulterated syrup by beet syrup, rice syrup and cassava syrup. by using linear discriminant analysis and support vector machine for the identification model, comparing the full s ...201526904809
authentication of pineapple (ananas comosus [l.] merr.) fruit maturity stages by quantitative analysis of γ- and δ-lactones using headspace solid-phase microextraction and chirospecific gas chromatography-selected ion monitoring mass spectrometry (hs-spme-gc-sim-ms).headspace solid phase microextraction and chirospecific gas chromatography-mass spectrometry in selected ion monitoring mode (hs-spme-gc-sim-ms) allowed quantitative determination of δ-lactones (δ-c8, δ-c10) and γ-lactones (γ-c6, γ-c8, γ-c10). a stable isotope dilution assay (sida) with d7-γ-decalactone as internal standard was used for quantitative analysis of pineapple lactones that was performed at three progressing post-harvest stages of fully ripe air-freighted and green-ripe sea-freighted ...201525172740
patulin and its dietary intake by fruit juice consumption in iran.a survey was conducted to determine levels and dietary intake of patulin (pat) from fruit juices consumed in iran. pat content was determined using high performance liquid chromatography-diode array detection in 161 samples of fruit juices including apple, pineapple, pear, peach, pomegranate and white and red grape juices. results revealed the presence of pat in 16.1% of the samples examined, ranging from 5 to 190.7 µg/kg. average and median pat content in positive samples was 34.5 and 18.5 µg/k ...201525494337
spray drying of fruit and vegetable juices--a review.the main cause of spray drying is to increase the shelf life and easy handling of juices. in the present paper, the studies carried out so far on spray drying of various fruits and vegetables are reported. the major fruit juices dried are mango, banana, orange, guava, bayberry, watermelon, pineapple, etc. however, study on vegetable juices is limited. in spray drying, the major optimized parameters are inlet air temperature, relative humidity of air, outlet air temperature, and atomizer speed th ...201524915356
ripening-dependent metabolic changes in the volatiles of pineapple (ananas comosus (l.) merr.) fruit: ii. multivariate statistical profiling of pineapple aroma compounds based on comprehensive two-dimensional gas chromatography-mass spectrometry.ripening-dependent changes of pineapple volatiles were studied in a nontargeted profiling analysis. volatiles were isolated via headspace solid phase microextraction and analyzed by comprehensive 2d gas chromatography and mass spectrometry (hs-spme-gc×gc-qms). profile patterns presented in the contour plots were evaluated applying image processing techniques and subsequent multivariate statistical data analysis. statistical methods comprised unsupervised hierarchical cluster analysis (hca) and p ...201525651901
study on evaluation of silage from pineapple (ananas comosus) fruit residue as livestock feed.pineapple is a commercially important fruit crop grown in asian and african countries. pineapple fruit residue (pfr) accounts for more than 65% of the processed fruits, and its disposal is a major problem due to its high moisture and sugar content predisposing it to fungal growth and spoilage. silage technique was adopted to address this problem, and the pfr silage was evaluated for its feeding value. it was observed that on 15th day, the ph of pfr silage was 4.2-4.3 and lactic acid content was ...201525633915
a new lavandulol-related monoterpene in the sex pheromone of the grey pineapple mealybug, dysmicoccus neobrevipes.the grey pineapple mealybug, dysmicoccus neobrevipes, is a serious pest that attacks a variety of crops in tropical regions. recently, it was recorded on an island in southwestern japan, suggesting that its distribution is expanding. as a measure against this expansion, a monitoring tool is urgently needed. in this study we determined the structure of the sex pheromone of d. neobrevipes in order to develop an efficient lure for monitoring traps. volatiles collected from virgin adult females were ...201525618324
immune reactivities against gums.different kinds of gums from various sources enjoy an extremely broad range of commercial and industrial use, from food and pharmaceuticals to printing and adhesives. although generally recognized as safe by the us food and drug administration (fda), gums have a history of association with sensitive or allergic reactions. in addition, studies have shown that gums have a structural, molecular similarity to a number of common foods. a possibility exists for cross-reactivity.201525599187
effects of ultraviolet light (uv-c) and heat treatment on the quality of fresh-cut chokanan mango and josephine pineapple.the effects of ultraviolet (uv-c) and medium heat (70 °c) treatments on the quality of fresh-cut chokanan mango and josephine pineapple were investigated. quality attributes included physicochemical properties (ph, titratable acidity, and total soluble solids), ascorbic acid content (vitamin c), antioxidant activity, as well as microbial inactivation. consumers' acceptance was also investigated through sensory evaluation of the attributes (appearance, texture, aroma and taste). furthermore, shel ...201525586772
bromelain: a natural proteolytic for intra-abdominal adhesion prevention.peritoneal adhesions are pathological fibrous connections between peritoneal surfaces resulting from incomplete peritoneal repair. adhesions cause various health problems ranging from pelvic pain and bowel obstruction to infertility. to date, no effective agent exists for intra-abdominal adhesion prevention. bromelain is the crude extract of the pineapple and it has fibrinolytic, antithrombotic, and anti-inflammatory properties. bromelain has been shown to be effective for removing necrotic tiss ...201525573606
swarm motility of salmonella enterica serovar typhimurium is inhibited by compounds from fruit peel extracts.controlling spread of human pathogens on fresh produce is a top priority for public health reasons. isolation of compounds from agricultural waste that would control spread of human pathogens was explored using salmonella enterica serovar typhimurium as a model organism. in the environment, micro-organisms migrate as a 'community' especially when they move on moist surfaces. this type of motility is characterized as swarming motility. we examined extracts from agricultural waste such as soya bea ...201525422036
pineapple [ananas comosus (l.) merr].the efficacy of agrobacterium-mediated pineapple transformation technique has been improved (mean percentage of transgenic micro-shoots regenerated from initial callus explants up to 20.6%) using a novel encapsulation-based, antibiotic selection procedure. the detailed protocol using a standard plant transformation vector (pcambia1304) as reported in an 'elite' indian variety (queen) of pineapple [ananas comosus (l.) merr] can be applied to other varieties of pineapple for introgression of targe ...201525416266
application of diffusion ordered-1h-nuclear magnetic resonance spectroscopy to quantify sucrose in beverages.this work focuses on a quantitative analysis of sucrose using diffusion ordered-quantitative (1)h-nuclear magnetic resonance spectroscopy (dosy-qnmr), where an analyte can be isolated from interference based on its characteristic diffusion coefficient (d) in gradient magnetic fields. the d value of sucrose in deuterium oxide at 30°c was 4.9 × 10(-10)m(2)/s at field gradient pulse from 5.0 × 10(-2) to 3.0 × 10(-1)t/m, separated from other carbohydrates (glucose and fructose). good linearity (r(2) ...201525308635
transient elevation of serum 5-hiaa by dietary serotonin and distribution of 5-hiaa in serum protein fractions.dietary serotonin increases urinary secretion of 5-hiaa. a falsely elevated 5-hiaa may lead to incorrect suspicion of a neuroendocrine tumour. therefore, we determined the effect and duration of dietary serotonin on serum 5-hiaa concentration. we also studied the distribution of 5-hiaa in serum fractions.201525249663
effect of uv-c radiation on bioactive compounds of pineapple (ananas comosus l. merr.) by-products.the industrial processing of pineapple generates a high quantity of by-products. to reduce the environmental impact of these by-products and the inherent cost of their treatment, it is important to characterise and valorise these products, converting them into high added value products. ultra-violet radiation is one of the main sustainable sanitation techniques for fruits. since this radiation can induce plant stress which can promote the biosynthesis of bioactive compounds, it is important to e ...201524852602
interaction of polyphenol oxidase of solanum tuberosum with β-cyclodextrin: process details and applications.polysaccharides differing in structure and chemical nature were screened for their ability to bind non-covalently with polyphenol oxidase (ppo) from potato (as a model) and their effect on enzyme activity. all the polysaccharides selected inhibited the ppo but β-cyclodextrin showed maximum inhibition under optimum conditions. process details for the inhibition of ppo were studied with respect to concentration of β-cyclodextrin, temperature, ph, and time. higher inhibition constant and lower half ...201526187193
cost-effective defined medium for the production of polyhydroxyalkanoates using agricultural raw materials.according to the cost of carbon substrate for producing biopolymer of polyhydroxyalkanoates (phas) still has a barrier to extend in an industrial scale. the aim of this work was to evaluate the defined phas media containing the agricultural raw materials of pineapple and sugarcane to produce phas by bacillus strain. batch fermentation was carried out in flask scale to compare the efficiency of defined media using statistical methodology. the defined medium 8 of pineapple was achieved the highest ...201526185927
leaf margin phenotype-specific restriction-site-associated dna-derived markers for pineapple (ananas comosus l.).to explore genome-wide dna polymorphisms and identify dna markers for leaf margin phenotypes, a restriction-site-associated dna sequencing analysis was employed to analyze three bulked dnas of f1 progeny from a cross between a 'piping-leaf-type' cultivar, 'yugafu', and a 'spiny-tip-leaf-type' variety, 'yonekura'. the parents were both ananas comosus var. comosus. from the analysis, piping-leaf and spiny-tip-leaf gene-specific restriction-site-associated dna sequencing tags were obtained and desi ...201526175625
diagnosis of gastroesophageal reflux disease using real-time magnetic resonance imaging.a small angle (his angle) between the oesophagus and the fundus of the stomach is considered to act as flap valve and anti-reflux barrier. a wide angle results in dysfunction of the oesophagogastric junction and subsequently in gastroesophageal reflux disease (gerd). here, we used real-time magnetic resonance imaging (mri) at 50 ms resolution (20 frames per second) in 12 volunteers and 12 patients with gerd to assess transport of pineapple juice through the oesophagogastric junction and reflux d ...201526175205
use of pressure for improving storage quality of fresh-cut produce.the microflora of fresh-cut produce is comprised primarily of phytopathogenic and soilborne organisms, but the product could be contaminated with foodborne pathogens. populations of bacteria, molds, and yeasts associated with fresh-cut produce decreased to non-detectable levels following a high pressure (hp) treatment of 400 mpa for 10 min at room temperature, except for spore-forming bacteria such as bacillus spp. which were inactivated when subjected to 600 mpa at 60 °c for 10 min. the hp trea ...201526174398
discovery of precursor and mature micrornas and their putative gene targets using high-throughput sequencing in pineapple (ananas comosus var. comosus).micrornas (mirnas) are a class of small, endogenous non-coding rnas that negatively regulate gene expression, resulting in the silencing of target mrna transcripts through mrna cleavage or translational inhibition. mirnas play significant roles in various biological and physiological processes in plants. however, the mirna-mediated gene regulatory network in pineapple, the model tropical non-climacteric fruit, remains largely unexplored. here, we report a complete list of pineapple mature mirnas ...201526115767
toward systems-level analysis of agricultural production from crassulacean acid metabolism (cam): scaling from cell to commercial production.systems-level analyses have become prominent tools for assessing the yield, viability, economic consequences and environmental impacts of agricultural production. such analyses are well-developed for many commodity crops that are used for food and biofuel, but have not been developed for agricultural production systems based on drought-tolerant plants that use crassulacean acid metabolism (cam). we review the components of systems-level evaluations, and identify the information available for com ...201526094655
quality control of fruit juices by using organic acids determined by capillary zone electrophoresis with poly(vinyl alcohol)-coated bubble cell capillaries.an enhanced method for the determination of organic acids in several fruit juices by capillary zone electrophoresis (cze) with direct uv-vis detection has been developed in this work. first, a study with simulated real juice samples was done to find the best separation conditions. next, several commercial fruit juices were analyzed, and the organic acid contents were quantified in less than 12 min using a poly(vinyl alcohol)-coated fused-silica 'bubble cell' capillary. the present method is reli ...201526041236
efficacy of reverse micellar extracted fruit bromelain in meat tenderization.reverse micellar extraction (rme) was used for the separation and purification of bromelain from pineapple core and efficacy of rme purified bromelain (rmeb) in tenderization of beef meat was compared with that of commercial stem bromelain (csb). rme resulted in reasonably high bromelain activity recovery (85.0 %) and purification fold (4.0). reduction in meat toughness was higher in rmeb treated meat (52.1 %) compared to raw (control) and csb treated (26.7 %). significant increase in water hold ...201526028772
postharvest exogenous application of abscisic acid reduces internal browning in pineapple.internal browning (ib) is a postharvest physiological disorder causing economic losses in pineapple, but there is no effective control measure. in this study, postharvest application of 380 μm abscisic acid (aba) reduced ib incidence by 23.4-86.3% and maintained quality in pineapple fruit. aba reduced phenolic contents and polyphenol oxidase and phenylalanine ammonia lyase activities; increased catalase and peroxidase activities; and decreased o2(·-), h2o2, and malondialdehyde levels. this sugge ...201526007196
fruit peels support higher yield and superior quality bacterial cellulose production.fruit peels, also known as rinds or skins, are wastes readily available in large quantities. here, we have used pineapple (pa) and watermelon (wm) peels as substrates in the culture media (containing 5 % sucrose and 0.7 % ammonium sulfate) for production of bacterial cellulose (bc). the bacterial culture used in the study, komagataeibacter hansenii produced bc under static conditions as a pellicle at the air-liquid interface in standard hestrin and schramm (hs) medium. the yield obtained was ~3. ...201525957154
application of an aqueous two-phase micellar system to extract bromelain from pineapple (ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations.bromelain is a set of proteolytic enzymes found in pineapple (ananas comosus) tissues such as stem, fruit and leaves. because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. the present study focused on the recovery of bromelain from pineapple peel by liquid-liquid extraction in aqueous two-phase micellar systems (atpms), using triton x-114 (tx-114) and mcilvaine buffer, in the absence and presence of electrolytes cacl2 and ...201525919128
selective pruning in pineapple plants as means to reduce heterogeneity in fruit quality.heterogeneity in fruit quality (size and taste) is a major problem in pineapple production chains. the possibilities were investigated of reducing the heterogeneity in pineapple in the field by pruning slips on selected plants, in order to promote the fruit growth on these plants. slips are side shoots that develop just below the pineapple fruit during fruit development. two on-farm experiments were carried out in commercial fields in benin with a cultivar locally known as sugarloaf, to determin ...201525853027
experience shapes our odor perception but depends on the initial perceptual processing of the stimulus.the questions of whether configural and elemental perceptions are competitive or exclusive perceptual processes and whether they rely on independent or dependent mechanisms are poorly understood. to examine these questions, we modified perceptual experience through preexposure to mixed or single odors and measured the resulting variation in the levels of configural and elemental perception of target odor mixtures. we used target mixtures that were spontaneously processed in a configural or an el ...201525832188
electrodialytic removal of nitrate from pineapple juice: effect on selected physicochemical properties, amino acids, and aroma components of the juice.this study aimed at investigating the effect of nitrate removal from pineapple juice by electrodialysis (ed) on selected properties of the ed-treated juice. single-strength pineapple juice with reduced pulp content was treated by ed to reduce the nitrate concentration to 15, 10, or 5 ppm. after ed, the removed pulp was added to the ed-treated juice and its properties, including electrical conductivity, acidity, ph, total soluble solids (tss), color, amino acids, and selected aroma compounds, wer ...201525827307
structural characteristics of pineapple pulp polysaccharides and their antitumor cell proliferation activities.pineapple has a delicious taste and good health benefits. bioactive polysaccharides are important components of pineapple that might contribute to its health benefits. since little structural information on these polysaccharides is currently available, the aim of this study was to investigate their structural characteristics and bioactivities.201525820875
transcriptome sequence analysis of an ornamental plant, ananas comosus var. bracteatus, revealed the potential unigenes involved in terpenoid and phenylpropanoid biosynthesis.ananas comosus var. bracteatus (red pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. because of its complex genome, it is difficult to understand the molecular mechanisms involved in the growth and development. thus high-throughput transcriptome sequencing of ananas comosus var. bracteatus is necessary to generate large quantities of transcript sequences for the purpose of gene discovery and functional genomic studies.201525769053
determination of the phenolic composition from brazilian tropical fruits by uhplc-ms/ms.although brazil is the third largest fruit producer in the world, several specimens consumed are not well studied from the chemical viewpoint, especially for quantitative analysis. for this reason and the crescent employment of mass spectrometry (ms) techniques in food science we selected twenty-two phenolic compounds with important biological activities and developed an ultra-high performance liquid chromatography tandem mass spectrometry (uhplc-ms/ms) method using electrospray (esi) in negativ ...201525766829
the effect of nanoparticle permeation on the bulk rheological properties of mucus from the small intestine.the effectiveness of delivering oral therapeutic peptides, proteins and nucleotides is often hindered by the protective mucus barrier that covers mucosal surfaces of the gastrointestinal (gi) tract. encapsulation of active pharmaceutical ingredients (api) in nanocarriers is a potential strategy to protect the cargo but they still have to pass the mucus barrier. decorating nanoparticles with proteolytic enzymes has been shown to increase the permeation through mucus. here we investigate the effec ...201525758122
comparative study of pineapple juice as a negative oral contrast agent in magnetic resonance cholangiopancreatography.the aim of this study was to compare the image quality of magnetic resonance cholangiopancreatography (mrcp) using pineapple juice (pj) or ranitidine as negative oral contrast agents and no agent.201525738055
rapid differentiation of commercial juices and blends by using sugar profiles obtained by capillary zone electrophoresis with indirect uv detection.a method for the determination of sugars in several fruit juices and nectars by capillary zone electrophoresis with indirect uv-vis detection has been developed. under optimal conditions, commercial fruit juices and nectars from several fruits were analyzed, and the sugar and cyclamate contents were quantified in less than 6 min. a study for the detection of blends of high-value juices (orange and pineapple) with cheaper alternatives was also developed. for this purpose, different chemometric te ...201525719749
ripening-dependent metabolic changes in the volatiles of pineapple (ananas comosus (l.) merr.) fruit: i. characterization of pineapple aroma compounds by comprehensive two-dimensional gas chromatography-mass spectrometry.qualitative ripening-dependent changes of pineapple volatiles were studied via headspace solid-phase microextraction and analyzed by comprehensive two-dimensional gas chromatography quadrupole mass spectrometry (hs-spme-gc×gc-qms). early green-ripe stage, post-harvest ripened, and green-ripe fruits at the end of their commercial shelf-life were compared to air-freighted pineapples harvested at full maturity. in total, more than 290 volatiles could be identified by mass spectrometry and their lin ...201525701413
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