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enzymatic treatment and use of starters for the nutrient enhancement in fermented flour of red and white varieties of finger millet (eleusine coracana).two varieties of finger millet (eleusine coracana)-a tannin-containing red variety, co13, and nontannin white variety, co9-processed by treatment with enzymes (cellulase and hemicellulase) and fermentation with starters (from previously fermented finger millet batter), achieved the desirable goals of reduced fermentation time (12 h), increased acidity (2.2 to 2.4%), enhanced in vitro protein digestibility (ivpd) (14 to 26%), and mineral availability compared to 48 h uncontrolled natural fermenta ...199910552488
structure of the bifunctional inhibitor of trypsin and alpha-amylase from ragi seeds at 2.9 a resolution.the crystal structure of a bifunctional inhibitor of alpha-amylase and trypsin from the seeds of ragi (indian finger millet, eleusine coracana gaertneri) has been determined by an x-ray diffraction method. the inhibitor consists of 122 amino acids with five disulfide bridges and belongs to the plant alpha-amylase/trypsin-inhibitor family. this is the first crystal structure determination of a member of this family. the protein, purified from the seeds of ragi, has a molecular mass of 13300 da wi ...199910089391
evaluation of the nutritional quality and acceptability of fingermillet-based tempe as potential weaning foods in tanzania.six types of fingermillet (eleusine coracana)-based tempe were developed by incorporating either commonbeans, groundnuts, cowpeas, mungbeans, chickpeas, sesame and/or their mixtures and fermented by rhizopus oligosporus. the proximate and mineral composition was not changed significantly by fermentation. the protein content of tempe ranged from 13.3 to 15.7%; the total energy, 374.1 to 434.3 kcal/100 g; the protein energy, 13.0 to 15.6%; the net dietary protein energy, 7.2 to 8.7%; and the chemi ...199910719573
genetic variability and diversity for protein and calcium contents in finger millet (eleusine coracana (l.) gaertn) in relation to grain color.analysis of 36 genotypes of finger millet (eleusine coracana (l.) gaertn) with varying seed colors revealed a wide range of protein and calcium contents. white seeded genotypes had higher protein contents, while brown seeded types had a wide range of values. the brown seeded genotype ge 2500 had the highest protein content. although protein content had significant negative association with calcium content, white seeded types had moderate levels of calcium. the genotypic coefficients of variabili ...199810426122
rbi, a one-domain alpha-amylase/trypsin inhibitor with completely independent binding sites.the bifunctional inhibitor from ragi (eleusine coracana gaertneri) (rbi) is the only member of the alpha-amylase/trypsin inhibitor family that inhibits both trypsin and alpha-amylase. here, we show that both enzymes simultaneously and independently bind to rbi. the recently solved three-dimensional nmr structure of rbi has revealed that the inhibitor possesses a hitherto unknown fold for serine proteinase and alpha-amylase inhibitors. despite its different fold, rbi obeys the standard mechanism ...19968941704
determination of the three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor from ragi seeds by nmr spectroscopy.the three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (rbi) from seeds of ragi (eleusine coracana gaertneri) has been determined in solution using multidimensional 1h and 15n nmr spectroscopy. the inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant alpha-amylase/trypsin inhibitor family for which no three-dimensional structures have yet been available. the structure of the inhibitor was determined on the basis of 1131 interresid ...19957599120
assessment of genome origins and genetic diversity in the genus eleusine with dna markers.finger millet (eleusine coracana), an allotetraploid cereal, is widely cultivated in the arid and semiarid regions of the world. three dna marker techniques, restriction fragment length polymorphism (rflp), randomly amplified polymorphic dna (rapd), and inter simple sequence repeat amplification (issr), were employed to analyze 22 accessions belonging to 5 species of eleusine. an 8 probe--3 enzyme rflp combination, 18 rapd primers, and 6 issr primers, respectively, revealed 14, 10, and 26% polym ...19957672607
evolution of finger millet: evidence from random amplified polymorphic dna.finger millet (eleusine coracana ssp. coracana) is an annual tetraploid member of a predominantly african genus. the crop is believed to have been domesticated from the tetraploid e. coracana ssp. africana. cytogenetic and isozyme data point to the allopolyploid nature of the species and molecular information has shown e. indica to be one of the genomic donors. a recent isozyme study questioned the proposed phylogenetic relationship between finger millet and its direct ancestor subspecies africa ...19957774797
stability and kinetics of a bifunctional amylase/trypsin inhibitor.the stability of the bifunctional amylase/trypsin inhibitor from ragi (indian finger millet, eleusine coracana) has been studied by methods of circular dichroism, uv absorption and intrinsic fluorescence. the inhibitor is stable in 8 m urea and 6 m guanidine-hcl. in 150 mm nacl, thermal denaturation does not occur up to 90 degrees c. however, it is irreversibly denatured in 5 mm nacl if heated over 73 degrees c. the acidic denaturation is reversible in both high and low salt conditions, but it s ...19937692971
glycaemic index of some commonly consumed foods in western india.glycaemic index (gi) was determined in 36 non-insulin-dependent diabetes mellitus (niddm) patients. the subjects were fed 50g carbohydrate portions of six foods consumed widely in india including varagu (plaspalum scorbiculatum) alone and in combination with whole and dehusked greengram (phaseolus aureus roxb), bajra (penniseteum typhoideum), jowar (sorghum vulgare) and ragi (eleusine coracana). the gi of varagu alone, varagu in combination with whole greengram and bajra was significantly lower ...199324352140
host genotype dependency and growth enhancing ability of va-mycorrhizal fungi for eleusine coracana (finger millet).inoculation of finger millet (eleusine coracana gaertn.) plants with one of six different vesicular, arbuscular, mycorrhizal (vam) fungi increased plant biomass, height, leaf area and absolute growth rate; however, effectiveness of the various vam fungi varied significantly. maximum root colonization and mycorrhizal efficacy was observed with plants inoculated with glomus caledonicum. among five host genotypes tested for mycorrhizal dependency against g. caledonicum, genotype hr-374 gave the hig ...199324419945
the 'a' genome donor of eleusine coracana (l.) gaertn. (gramineae).in an attempt to discover 'a' and 'b' genome donor(s) to finger millet, eleusine coracana, or its progenitor species, e. africana (both allotetraploid 2n=4x=36), five diploid species, e. indica, e. floccifolia, e. multiflora, e. tristachya and e. intermedia, were crossed to finger millet and its progenitor taxon. crosses were successful only with e. coracana. three combinations of triploid hybrids e. coracana x e. indica, e. coracana x e. floccifolia, and e. coracana x e. multiflora were obtaine ...199224201369
identification of the three major coeliac immunoreactive proteins and one alpha-amylase inhibitor from oat endosperm.six chloroform/methanol-soluble proteins from oat endosperm (avena sativa) have been isolated and characterized by a purification procedure based on extraction with volatile solvents, followed by reversed-phase high performance liquid chromatography. three of these proteins, with an assessed molecular weight of 25,000, 27,000 and 32,000 da, respectively, have been identified by immunoblotting using coeliac sera, as the major coeliac serum iga-binding components of oat endosperm. the n-terminal a ...19921526282
food-based solutions are a viable alternative to glucose-electrolyte solutions for oral hydration in acute diarrhoea--studies in a rat model of secretory diarrhoea.a survey of acute diarrhoea and its treatment, in 3 groups of villages in south india, revealed that use of the world health organization oral rehydration solution (who-ors) was poor or virtually non-existent and that several liquid foods were given to children during acute diarrhoea. the effects of the most commonly used, boiled and cooled supernatants of these liquid foods [rice (oryza sativa)-water, ragi (eleusine coracana)-water, arrowroot (maranta arundinacea)-water], and tender coconut-wat ...19902345922
a population-based case-control investigation on cancers of the oral cavity in bangalore, india.a case-control study on cancers of the oral cavity was conducted by utilising data from the population based cancer registry. bangalore, india. three hundred and forty-eight cases of cancers of the oral cavity (excluding base tongue) were age and sex matched with controls from the same residential area but with no evidence of cancer. the relative risk due to pan tobacco chewing was elevated in both males and females, being appreciably higher in the latter (relative risk 25.3%; 95% confidence int ...19902245179
natural occurrence of alternaria mycotoxins in sorghum and ragi from north bihar, india.the natural occurrence of major alternaria mycotoxins i.e. alternariol (aoh), alternariol methyl ether (ame), altenuene (alt), altertoxin-1 (atx-1) and tenuazonic acid (ta) has been investigated in sorghum (sorghum vulgare pers.) and ragi (eleusine coracana gaertn.) collected from north bihar. nine out of 20 sorghum samples, and three out of eight ragi samples, were found to be contaminated with one to three alternaria mycotoxins. the toxin elaborating potential of alternaria alternata (fr.) kei ...19902079114
aspartate aminotransferase from eleusine coracana, a c4 plant: purification, characterization, and preparation of antibody.aspartate aminotransferase (aspat) isozymes from eleusine coracana (an nad-malic enzyme type c4 plant) were examined. three groups of isoenzymes were identified (aspat-1, aspat-2, and aspat-3). aspat-1 (localized in the mesophyll cells) and aspat-3 (localized in the bundle sheath cells), both of which are considered to function in the c4 acid pathway, were purified and their kinetic and physical properties studied. both isoenzymes had a molecular mass of 80 kda and were shown to consist of two i ...19902241160
high frequency embryoid and plantlet formation from tissue cultures of the finger millet-eleusine coracana (l.) gaertn.compact nodulated embryogenic callus differentiated from cultured seeds of eleusine coracana (finger millet) on murashige and skoog (1962) basal medium with 2,4-dichlorophenoxyacetic acid (1.0, 3.0 mg (l)). this embryogenic callus was maintained on a medium with a lower level of 2,4 - dichlorophenoxyacetic acid. at every subculture the embryogenic callus had some preexisting embryoids in it. with this method of subculture the callus has retained its morphogenic potential for four years. followin ...199024226438
purification and characterization of nad malic enzyme from leaves of eleusine coracana and panicum dichotomiflorum.nad malic enzyme (ec 1.1.1.39), which is involved in c(4) photosynthesis, was purified to electrophoretic homogeneity from leaves of eleusine coracana and to near homogeneity from leaves of panicum dichotomiflorum. the enzyme from each c(4) species was found to have only one type of subunit by sds polyacrylamide gel electrophoresis. the m(r) of subunits of the enzme from e. coracana and p. dichotommiflorum was 63 and 61 kilodaltons, respectively. the native mr of the enzyme from each species was ...198916666533
in vitro availability of iron and zinc in white and coloured ragi (eleusine coracana): role of tannin and phytate.white and brown ragi (eleusine coracana) varieties were analysed for tannin, phytate phosphorus, total phosphorus, iron, ionisable iron, zinc and soluble zinc content. white ragi had no detectable tannin while in brown varieties it ranged from 351 to 2392 mg per 100 g. germination brought about a progressive decrease in tannin and phytate phosphorus and an increase in ionisable iron and soluble zinc content of grain ragi. both in raw and germinated grain, ionizable iron was significantly higher ...19883231591
selective expression of a probable amylase/protease inhibitor in barley aleurone cells: comparison to the barley amylase/subtilisin inhibitor.we have cloned and sequenced a 650-nucleotide cdna from barley (hordeum vulgare l.) aleurone layers encoding a protein that is closely related to a known α-amylase inhibitor from indian finger millet (eleusine coracana gaertn.), and that has homologies to certain plant trypsin inhibitors. mrna for this probable amylase/protease inhibitor (papi) is expressed primarily in aleurone tissue during late development of the grain, as compared to that for the amylase/subtilisin inhibitor, which is expres ...198624232429
2'-o-methyl-1-methyl adenosine: a new modified nucleoside in ragi (eleusine coracana) trna.a new modified nucleoside 2'-o-methyl-l-methyl adenosine has been found to be present in the trna of eleusine coracana ( ragi ) seedlings. the sequence of the dinucleotide of which this modified nucleoside is a part suggests its presence in phenylalanine-trna. the structural implications of the presence of this new modification are discussed.19846547340
age-related changes in catalase and peroxidase activities in the excised leaves of eleusine coracana gaertin. cv pr 202 during senescence.changes in the activities of the enzymes catalase and peroxidase were studied in the excised leaves of ragi (eleusine coracana gaertn. cv pr 202) plants belonging to different ages. catalase exhibited a positive and peroxidase a negative correlation with the changes in chlorophyll. catalase and peroxidase were negatively correlated with each other. peroxidase exhibited an age-related drift in its activity. kinetin could maintain the levels of chlorophyll and catalase, and also caused an increase ...19836321215
effect of insecticides, oxydementon-methyl & dimethoate, on chlorophyll retention & hydrogen peroxide utilization in ragi (eleusine coracana gaertn.cv pr 202) leaves during senescence. 19827183525
natural plant enzyme inhibitors. characterization of an unusual alpha-amylase/trypsin inhibitor from ragi (eleusine coracana geartn.).an inhibitor i-1, capable of acting on both alpha-amylase and trypsin, was purified to homogeneity from ragi (finger-millet) grains. the factor was found to be stable to heat treatment at 100 degrees c for 1 h in the presence of nacl and also was stable over the wide ph range 1-10. pepsin and pronase treatment of inhibitor i-1 resulted in gradual loss of both the inhibitory activities. formation of trypsin-inhibitor i-1 complex, amylase-inhibitor i-1 complex and trypsin-inhibitor i-1-amylase tri ...19816796040
repetitive dna in three gramineae species with low dna content.the genomes of three gramineae species, namely finger millet (eleusine coracana), pearl millet (pennisetum americanum) and rice (oryza sativa) are characterized by studying their dna denaturation-reassociation properties. the reassociation kinetics measurement of the sonicated dna (500--700 nucleotide pairs) indicate the presence of a heterogeneous, repetitive dna fraction accounting for 49--54% of the total dna in all three species. from the cot 1/2 value of the slow reassociating dna, the geno ...19807409756
proteinase inhibitors of finger millet (eleusine coracana gaertn.). 1978661229
sterol lipids in finger millet (eleusine coracana). 1978701708
relationship between tannin levels and in vitro protein digestibility in finger millet (eleusine coracana gaertn.). 1977893835
studies on liver sulphurylase activity in rats: vitamin-a-deficient and low-protein diets.studies on the sulphurylation of phenols by livers from normal and vitamin-a-deficient rats showed a significant reduction in sulphurylating activity in the acute stage of deficiency. at the 'plateau stage' of deficiency, decrease in this activity was marginal and was not significant. rats consuming a 6% casein diet had a significantly lower liver sulphurylating activity when compared to those fed 12 and 18% casein diets. comparative data on the sulphurylating activity of rats maintained on diet ...19751232600
biophysical characterization of mesophyll and bundle sheath chloroplasts isolated from the leaves of eleusine coracana, an aspartate-type c-4 plant : part i. light absorbing photosynthetic pigment composition. 19751218880
photorespiratory metabolism in eleusine coracana, a c-4 plant. 19744468932
induced interchanges in finger millet (eleusine coracana gaert). 19744824618
effects of supplementing ragi (eleusine coracana) diets with lysine or leaf protein on the growth and nitrogen metabolism of children. 19695357042
the metabolism of nitrogen, calcium and phosphorus in undernourished children. v. the effect of partial or complete replacement of rice in poor vegetarian diets by ragi (eleusine coracana) on the metabolism on nitrogen, calcium and phosphorus. 195913662551
the metabolism of nitrogen, calcium and phosphorus in human adults on a poor vegetarian diet containing ragi (eleusine coracana). 195513284215
effects of partial replacement of rice, wheat or ragi (eleusine coracana) by tuber flours on the nutritive value of poor vegetarian diets. 195413140823
the effects on growth in rats of butter and ragi (eleusine coracana), separately and combined, as supplements to the poor rice diet of south india. 194620999651
the dicarboxylic acid-nitrogen of proteins: with a note on the alcohol-soluble protein from ragi (eleusine coracana). 193116744782
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