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the role of climatic variables in winter cereal yields: a retrospective analysis.this study examined the effects of observed climate including [co2] on winter cereal [winter wheat (triticum aestivum), barley (hordeum vulgare) and oat (avena sativa)] yields by adopting robust statistical analysis/modelling approaches (i.e. autoregressive fractionally integrated moving average, generalised addition model) based on long time series of historical climate data and cereal yield data at three locations (moree, dubbo and wagga wagga) in new south wales, australia. research results s ...201524763659
preferential recruitment of the maternal centromere-specific histone h3 (cenh3) in oat (avena sativa l.) × pearl millet (pennisetum glaucum l.) hybrid embryos.chromosome elimination occurs frequently in interspecific hybrids between distantly related species in poaceae. however, chromosomes from both parents behave stably in a hybrid of female oat (avena sativa l.) pollinated by pearl millet (pennisetum glaucum l.). to analyze the chromosome behavior in this hybrid, we cloned the centromere-specific histone h3 (cenh3) genes of oat and pearl millet and produced a pearl millet-specific anti-cenh3 antibody. application of this antibody together with a gr ...201526134441
mitochondrial structural and antioxidant system responses to aging in oat (avena sativa l.) seeds with different moisture contents.we observed the relationship between lifespan and mitochondria, including antioxidant systems, ultrastructure, and the hydrogen peroxide and malondialdehyde contents in 4 h imbibed oat (avena sativa l.) seeds that were aged with different moisture contents (4%, 10% and 16%) for 0 (the control), 8, 16, 24, 32 and 40 d at 45 °c. the results showed that the decline in the oat seed vigor and in the integrity of the mitochondrial ultrastructure occurred during the aging process, and that these change ...201526079285
evaluation of post-germinative lipid peroxidation and enzymatic antioxidant potential in lead absorbing oat (avena sativa) seedlings.the objective of the present research was to study the impact of lead (pb) on growth, metal uptake and antioxidative potential of oat seeds under metal stress. to achieve these objectives, few experiments were conducted to assess the effect of this particular metal on various anti-oxidative enzymes, during initial metabolism after germination, in presence of lead. pb is not an oxido-reducing metal, the oxidative stress induced by pb in growing oat seedlings appears to be an indirect effect of pb ...201526536805
ecological risk assessment of a metal-contaminated area in the tropics. tier ii: detailed assessment.this study presents data on the detailed evaluation (tier 2) of a site-specific ecological risk assessment (ssera) in a former smelter area contaminated with metals (santo amaro, bahia, brazil). combining information from three lines of evidence (loe), chemical (chemloe), ecotoxicological (ecotoxloe) and ecological (ecoloe), in the triad approach, integrated risk values were calculated to rank sites and confirm the potential risk disclosed with tier 1. risk values were calculated for the habitat ...201526528915
optimised amylases extraction from oat seeds and its impact on bread properties.statistical approaches were employed for the optimisation of the extraction of amylolytic activity from oat (avena sativa) seeds. the application of the response surface methodology allows us to determine a set of optimal conditions (ratio seed weight/buffer volume 0.1, germination days 10 days, temperature 20 °c and ph 5.6). experiments carried out under these conditions led to amylase production yield of 91 u/g. its maximal activity was in the ph 5.6 and at 55 °c. study of the incorporation of ...201525453287
uptake and distribution of stable strontium in 26 cultivars of three crop species: oats, wheat, and barley for their potential use in phytoremediation.the main objective of this study was to investigate the accumulation and distribution of strontium (sr) in 26 cultivars of wheat (triticum aestivum l.), husk oat (avena sativa l) and naked oat (avena nuda), and barley (hordeum vulgare l.) for their potential use in phytoremediation.sr levels had no effect on the accumulation of shoot biomass at tillering or at maturity. mean shoot sr concentration of naked oat and barley at tillering was significantly (p<0.05) higher than that of wheat; neimengk ...201525397985
optogenetics: optical control of a photoactivatable rac in living cells.recent developments in optogenetics have extended optical control of signaling to intracellular proteins, including rac, a small g protein in the rho family. a blue light-sensing lov (light, oxygen, or voltage) domain derived from avena sativa (oat) phototropin was fused to the n-terminus of a constitutively active mutant of rac, via an α-helix (jα) that is conserved among plant phototropins. the fused lov domain occluded binding of downstream effectors to rac in the dark. exposure to blue light ...201525391805
cholesterol-lowering properties of oat β-glucan and the promotion of cardiovascular health: did health canada make the right call?in 2010, health canada approved a heath claim acknowledging the link between increased oats (avena sativa)-soluble fibre consumption and a reduction in total serum cholesterol levels. the approval also recognized the relationship between decreased total blood cholesterol concentration and a reduced risk of coronary heart disease. the functional food ingredient believed to be responsible for the hypocholesterolemic property of oats is β-glucan, a highly viscous, soluble fibre composed of d-glucos ...201525933163
characterization of (glucurono)arabinoxylans from oats using enzymatic fingerprinting.cell wall material from whole oat grains was sequentially extracted to study the structural characteristics of individual arabinoxylan (ax) populations. araf was singly substituted at both o-3 (mainly) and o-2 positions of xylp, and no disubstitution of xylp with araf residues was found in oat axs. both highly substituted and sparsely substituted segments were found in axs in ba(oh)2 extracts, whereas axs in 1 and 6 m naoh extracts were rarely branched and easily aggregated. both o-2-linked glca ...201526636262
influence of crop residues on trifluralin mineralization in a silty clay loam soil.trifluralin is typically applied onto crop residues (trash, stubble) at the soil surface, or onto the bare soil surface after the incorporation of crop residues into the soil. the objective of this study was to quantify the effect of the type and amount of crop residues in soil on trifluralin mineralization in a wellwood silty clay loam soil. leaves and stubble of potato (solanum tuberosum) (p); canola (brassica napus) (c), wheat (triticum aestivum) (w), oats (avena sativa), (o), and alfalfa (me ...201517454379
[isolation and structure of peptides from oat (avena sativa) seedlings].the level of proteolytic activity in tissues of oat seedlings was characterized under acidic conditions, and the number and content of the main components in low-molecular-mass fractions of the extract were determined. the structures of the majority of predominant peptide components isolated from the extract were studied. the use of a database of protein structures helped suggest possible structures of protein precursors of the peptides isolated. detailed information on a plant peptidome was obt ...201516637293
[genetic analysis of oat (avena sativa l.) by joint scaling test].comparing the results of genetic analysis of oat varieties with the method of diallel analysis of f1 hybrids and with the joint scaling test the spheres of optimal application of both methods were determined. quantitative estimation of genetic parameters forming the phenotypic averages in scaling test develops the necessary base for involvement of marker genes for qualitative characters in search of qtls controlling the traits with continuous variation. the crosses being the most suitable for fu ...201514650325
apatite and phillipsite as sequestering agents for metals and radionuclides.laboratory and greenhouse studies were conducted to quantify apatite and phillipsite (zeolite) sequestration of selected metal contaminants. the laboratory batch study measured the sorption of aqueous co2+, ba2+, pb2+, eu3+, and uo2(2+). apatite sorbed more co2+, pb2+, eu3+, and uo2(2+) from the spike solution than phillipsite, resulting in distribution coefficients (kd values) of >200,000 l kg(-1). phillipsite was more effective than apatite at sorbing aqueous ba2+. results from the laboratory ...201512708675
amelioration of nickel phytotoxicity in muck and mineral soils.in situ remediation (phytostabilization) is a cost-effective solution for restoring the productivity of metal-contaminated soils and protection of food chains. a pot experiment with wheat (triticum aestivum l.), oat (avena sativa l.), and redbeet (beta vulgaris l.) was conducted to test the ability of limestone and hydrous ferric oxide (hfo) to ameliorate ni phytotoxicity in two soils contaminated by particulate emissions from a nickel refinery. quarry muck (terric haplohemist; 72% organic matte ...201511790001
modulating effects of oatmeal extracts in the sodium lauryl sulfate skin irritancy model.the aim of the present study was to assess the anti-inflammatory activity of two topically applied oatmeal extracts, i.e. avena sativa and avena rhealba, using the sodium lauryl sulfate (sls) irritation model. at baseline, test areas on the volar surface of the upper arms of 12 healthy individuals were pretreated with the two extracts and their vehicle (petrolatum ointment) under occlusion for 2 h, and one site was left untreated. then a patch with a 1% sls solution was applied to the test sites ...201511867969
[kinetics of mitotic rhythmic activity in oat (avena sativa) coleoptiles following irradiation].the analysis of different intensity (10-200 gy) irradiation influence on rhythmical processes in mitotic activity of oats coleoptiles was carries out. obtained results indicate that irradiation disorders phase and amplitude of the cell division rhythm in investigated material. this disturbance, as judged by doze dependence, is associated with unknown molecular structures.201511833332
bacterially produced pt-gfp as ratiometric dual-excitation sensor for in planta mapping of leaf apoplastic ph in intact avena sativa and vicia faba.ratiometric analysis with h(+)-sensitive fluorescent sensors is a suitable approach for monitoring apoplastic ph dynamics. for the acidic range, the acidotropic dual-excitation dye oregon green 488 is an excellent ph sensor. long lasting (hours) recordings of apoplastic ph in the near neutral range, however, are more problematic because suitable ph indicators that combine a good ph responsiveness at a near neutral ph with a high photostability are lacking. the fluorescent ph reporter protein fro ...201425313311
vitamin e levels in soybean (glycine max (l.) merr.) expressing a p-hydroxyphenylpyruvate gene from oat (avena sativa l.).the enzyme p-hydroxyphenylpyruvate dioxygenase (hppd) is ubiquitous in plants and functions in the tyrosine catabolic pathway, resulting in the formation of homogentisate. homogentisate is the aromatic precursor of all plastoquinones and tocochromanols, including tocopherols and tocotrienols. soybean (glycine max (l.) merr.) has been genetically modified to express the gene avhppd-03 that encodes the protein avhppd-03 derived from oat (avena sativa l.). the avhppd-03 isozyme has an inherent redu ...201424684596
structural studies of arabinan-rich pectic polysaccharides from abies sibirica l. biological activity of pectins of a. sibirica.highly branched arabinan-rich pectic polysaccharides, containing 84% of arabinose, was extracted from wood greenery of abies sibirica l. the structure of arabinan was studied by the 1d and 2d nmr spectroscopy. the macromolecule backbone was represented mainly by rg-i (molar ratio gala:rha ∼ 1.3:1) patterns with high degree of rhamnose branching. side chains were comprised of 1,5-linked α-l-araf residues (the major part of polymer mass), 1,3,5-di-o- and 1,2,3,5-tri-o-linked α-l-araf residues, con ...201425256514
biomechanics of plant anchorage at early development stage.we propose a minimal model for the response of seedlings to pullout constraints. central to our approach is the idea of capturing not only average mechanical properties but also the stochastic component of the uprooting process. our model accounts on one hand for the tensile elastic response of root fibres and on the other hand for the friction between root fibres and the soil matrix. we present for validation a dataset of 98 uprooting experiments using avena sativa l. seedlings (common oat), gr ...201425109590
in silico analysis of dreb transcription factor genes and proteins in grasses.plants are exposed to various environmental stresses, including drought, salinity, low temperature, etc. dehydration responsive element binding (dreb) genes, the members of ap2/erf transcription factor family, regulate the biological processes against cold and dehydration stresses. in this study, we analyzed a total of 19 dreb transcription factor genes and proteins from 14 grass species by using bioinformatic approaches, including their physiochemical properties, conserved motif structures, hom ...201425104001
using genotyping-by-sequencing (gbs) for genomic discovery in cultivated oat.advances in next-generation sequencing offer high-throughput and cost-effective genotyping alternatives, including genotyping-by-sequencing (gbs). results have shown that this methodology is efficient for genotyping a variety of species, including those with complex genomes. to assess the utility of gbs in cultivated hexaploid oat (avena sativa l.), seven bi-parental mapping populations and diverse inbred lines from breeding programs around the world were studied. we examined technical factors t ...201425047601
engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells.the function of many eukaryotic proteins is regulated by highly dynamic changes in their nucleocytoplasmic distribution. the ability to precisely and reversibly control nuclear translocation would, therefore, allow dissecting and engineering cellular networks. here we develop a genetically encoded, light-inducible nuclear localization signal (linus) based on the lov2 domain of avena sativa phototropin 1. linus is a small, versatile tag, customizable for different proteins and cell types. linus-m ...201425019686
protective effects of oat oil on deltamethrin-induced reprotoxicity in male mice.oats (avena sativa l.), which are used in foods, are a potential economically viable source of oil. the purpose of this study was to determine the efficiency of oats oil to alleviate oxidative damage of testis induced by deltamethrin, which is a pyrethroid pesticide that exerts a wide range of effects on non-targeted organisms. the reprotoxicity caused by orally administered deltamethrin (del) to mice can be effectively antagonized by the beneficial effects of oats oil (oo) as an antioxidant. th ...201424992227
factors controlling carbon metabolism and humification in different soil agroecosystems.the aim of this study was to describe the processes that control humic carbon sequestration in soil. three experimental sites differing in terms of management system and climate were selected: (i) abanilla-spain, soil treated with municipal solid wastes in mediterranean semiarid climate; (ii) puch-germany, soil under intensive tillage and conventional agriculture in continental climate; and (iii) alberese-italy, soil under organic and conventional agriculture in mediterranean subarid climate. th ...201425614887
the spectrum of major seed storage genes and proteins in oats (avena sativa).the oat seed storage proteins are mainly composed of two classes: the globulins and avenins. among the major cereals, the globulins are the major seed protein class in rice and oats, and along with the higher protein content of oats is the basis for the relative higher nutrition content in oats compared to the other cereals. the second major class of oat seed proteins is the avenins; also classified as prolamins - seed proteins high in proline and glutamine amino acids. the prolamins are associa ...201425054628
biophysical, mutational, and functional investigation of the chromophore-binding pocket of light-oxygen-voltage photoreceptors.as light-regulated actuators, sensory photoreceptors underpin optogenetics and numerous applications in synthetic biology. protein engineering has been applied to fine-tune the properties of photoreceptors and to generate novel actuators. for the blue-light-sensitive light-oxygen-voltage (lov) photoreceptors, mutations near the flavin chromophore modulate response kinetics and the effective light responsiveness. to probe for potential, inadvertent effects on receptor activity, we introduced thes ...201424926890
manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides.optogenetic control of endogenous signaling can be an important tool for probing cell behavior. using the photoresponse of the lov2 domain of avena sativa phototropin 1, we developed analogues of kinase inhibitors whose activity is light dependent. inhibitory peptides were appended to the jα helix, where they potently inhibited kinases in the light but were sterically blocked from kinase interaction in the dark. photoactivatable inhibitors for cyclic-amp dependent kinase (pka) and myosin light c ...201424905630
metabolic changes in avena sativa crowns recovering from freezing.extensive research has been conducted on cold acclimation and freezing tolerance of fall-sown cereal plants due to their economic importance; however, little has been reported on the biochemical changes occurring over time after the freezing conditions are replaced by conditions favorable for recovery and growth such as would occur during spring. in this study, gc-ms was used to detect metabolic changes in the overwintering crown tissue of oat (avena sativa l.) during a fourteen day time-course ...201424675792
cover crops influence soil microorganisms and phytoextraction of copper from a moderately contaminated vineyard.we investigated the ability of summer (avena sativa [oat], trifolium incarnatum [crimson clover], chenopodium [goosefoot]) and winter (vicia villosa [hairy vetch], secale cereale l. [rye], brassica napus l. partim [rape]) cover crops, including a mixed species treatment, to extract copper from an organic vineyard soil in situ and the microbial communities that may support it. clover had the highest copper content (14.3mgcukg(-1) dm). however, it was the amount of total biomass production that de ...201425217742
preferred carbon precursors for lipid labelling in the heterotrophic endosperm of developing oat (avena sativa l.) grains.oat (avena sativa l.) is unusual among the cereal grains in storing high amounts of oil in the endosperm; up to 90% of total grain oil. by using oat as a model species for oil metabolism in the cereal endosperm, we can learn how to develop strategies to redirect carbon from starch to achieve high-oil yielding cereal crops. carbon precursors for lipid synthesis were compared in two genetically close oat cultivars with different endosperm oil content (about 6% and 10% of grain dw, medium-oil; mo, ...201425221923
fall-grown oat to extend the fall grazing season for replacement dairy heifers.our objective was to assess the pasture productivity and forage characteristics of 2 fall-grown oat (avena sativa l.) cultivars, specifically for extending the grazing season and reducing reliance on harvested forages by replacement dairy heifers. a total of 160 gravid holstein heifers (80 heifers/yr) were stratified by weight, and assigned to 1 of 10 identical research pens (8 heifers/pen). initial body weights were 480 ± 43.5 kg in 2011 and 509 ± 39.4 kg in 2012. during both years of the trial ...201424440262
assessing crop residue phosphorus speciation using chemical fractionation and solution 31p nuclear magnetic resonance spectroscopy.at physiological maturity, nutrients in crop residues can be released to the soil where they are incorporated into different labile and non-labile pools while the remainder is retained within the residue itself. the chemical speciation of phosphorus (p) in crop residues is an important determinant of the fate of this p. in this study, we used chemical fractionation and (31)p nuclear magnetic resonance (nmr) spectroscopy, first separately and then together, to evaluate the p speciation of mature ...201424881542
effect of different processing techniques on nutritional characteristics of oat (avena sativa) grains and formulated weaning mixes.one of the variety of oat, upo 94 was processed by two simple processing techniques. one method is malting and other is roasting. malted and roasted oat flour as well as weaning mixes prepared from these two processed flour were analyzed for their nutrient composition, alpha amylase activity and in-vitro protein digestibility. for making weaning mix, oat flour, wheat flour, green gram and skim milk powder were taken in a ratio of 30:30:25:15 respectively. higher moisture, ash, crude fat, energy, ...201425190893
factors that control the chemistry of the lov domain photocycle.algae, plants, bacteria and fungi contain light-oxygen-voltage (lov) domains that function as blue light sensors to control cellular responses to light. all lov domains contain a bound flavin chromophore that is reduced upon photon absorption and forms a reversible, metastable covalent bond with a nearby cysteine residue. in avena sativa lov2 (aslov2), the photocycle is accompanied by an allosteric conformational change that activates the attached phototropin kinase in the full-length protein. b ...201424475227
detecting a new source for photochemically induced dynamic nuclear polarization in the lov2 domain of phototropin by magnetic-field dependent (13)c nmr spectroscopy.phototropin is a flavin mononucleotide (fmn) containing blue-light receptor, which regulates, governed by its two lov domains, the phototropic response of higher plants. upon photoexcitation, the fmn cofactor triplet state, (3)f, reacts with a nearby cysteine to form a covalent adduct. cysteine-to-alanine mutants of lov domains instead generate a flavin radical upon illumination. here, we explore the formation of photochemically induced dynamic nuclear polarization (cidnp) in lov2-c450a of avena ...201425207844
increased productivity of a cover crop mixture is not associated with enhanced agroecosystem services.cover crops provide a variety of important agroecological services within cropping systems. typically these crops are grown as monocultures or simple graminoid-legume bicultures; however, ecological theory and empirical evidence suggest that agroecosystem services could be enhanced by growing cover crops in species-rich mixtures. we examined cover crop productivity, weed suppression, stability, and carryover effects to a subsequent cash crop in an experiment involving a five-species annual cover ...201424847902
effect of gamma irradiation on the nutritional quality of agaricus bisporus strains cultivated in different composts.the effect of irradiation doses (0, 125, 250 and 500 gy) on the nutritional quality of a. bisporus mushrooms (strains abi-07/06, abi-05/03 and pb-1) cultivated in composts based on oat straw (avena sativa) and brachiaria (brachiaria sp.) was evaluated. the experimental design was 4 x 3 x 2 factorial scheme (irradiation doses x strains x composts), with 24 treatments, consisting of two repetitions each, totaling 48 experimental units (samples of mushrooms). the samples were irradiated in cobalt-6 ...201424838546
interplanting annual ryegrass, wheat, oat, and corn to mitigate iron deficiency in dry beans.this study evaluated whether grass intercropping can be used to alleviate fe deficiency chlorosis in dry beans (phaseolus vulgaris l.) grown in high ph, calcareous soils with low organic matter. field studies were conducted at the university of wyoming sustainable agriculture research and extension center in 2009 and 2010. black- and navy beans were grown alone or intercropped with annual ryegrass (lolium multiflorum lam.), oat (avena sativa l.), corn (zea mays l.), or spring wheat (triticum aes ...201425536084
new series of avenanthramides in oat seed.avenanthramides are characteristic constituents of oat seeds. we analyzed the methanol extract of oat seeds by hplc and detected three compounds 1, 2, and 3 eluted at retention times similar to avenanthramides. the three compounds were purified by column chromatography and hplc. spectroscopic analyses of 1, 2, and 3 suggested that they are amides of 4,5-dihydroxyanthranilic acid with caffeic, p-coumaric, and ferulic acids, respectively. their identities were confirmed by comparing spectra and ch ...201425117953
exploring the origin of the d genome of oat by fluorescence in situ hybridization.further understanding of the origin of cultivated oat would accelerate its genetic improvement. in particular, it would be useful to clarify which diploid progenitor contributed the d genome of this allohexaploid species. in this study, we demonstrate that the landmarks produced by fluorescence in situ hybridization (fish) of species of avena using probes derived from avena sativa can be used to explore the origin of the d genome. selected sets of probes were hybridized in several sequential exp ...201425478818
physiological role of a multigrain diet in metabolic regulations of lipid and antioxidant profiles in hypercholesteremic rats: multigrain diet in hyperlipemia.the objective of the present study was to investigate the lipid and the antioxidant regulatory potential of a multigrain diet in laboratory animals with reference to lipid profiles, tissue lipid peroxidation and antioxidant status.201425780697
environmental nutrient supply alters prevalence and weakens competitive interactions among coinfecting viruses.the rates and ratios of environmental nutrient supplies can determine plant community composition. however, the effect of nutrient supplies on within-host microbial interactions is poorly understood. resource competition is a promising theory for understanding microbial interactions, because microparasites require nitrogen (n) and phosphorus (p) for synthesis of macromolecules such as nucleic acids and proteins. to better understand the effects of nutrient supplies to hosts on pathogen interacti ...201424975238
copper and selenium: auxiliary measure to control infection by haemonchus contortus in lambs.the aim of this study was to evaluate the effects of selenium and copper on oxidative stress and its performance in lambs experimentally infected with haemonchus contortus. twenty-eight five-months old lambs were experimentally infected by the oral route with 5000 third-stage infective larvae and allocated into four groups, i.e., untreated animals, animals treated intramuscularly with sodium selenite (0.2 mg kg(-1)), animals treated subcutaneously with copper (3.5 mg kg(-1)), and animals treated ...201424950380
evaluation of γ- aminobutyric acid, phytate and antioxidant activity of tempeh-like fermented oats (avena sativa l.) prepared with different filamentous fungi.tempeh is a popular traditional fermented food in asia. many tempeh-like foods are made from cereal grains. however, the information of γ-aminobutyric acid (gaba) accumulation in those tempeh-like cereal grains during fermentation is lacking. meanwhile, little information is available on the anti-nutrient contents and antioxidant activity of tempeh-like fermented oats. the aim of the present work was to study the changes of gaba, phytate, natural antioxidants and antioxidant activity of tempeh-l ...201425328194
the genetics of resistance to powdery mildew in cultivated oats (avena sativa l.): current status of major genes.the genetics of resistance to powdery mildew caused by blumeria graminis f. sp. avenae of four cultivated oats was studied using monosomic analysis. cultivar 'bruno' carries a gene (pm6) that shows a recessive mode of inheritance and is located on chromosome 10d. cultivar 'jumbo' possesses a dominant resistance gene (pm1) on chromosome 1c. in cultivar 'rollo', in addition to the gene pm3 on chromosome 17a, a second dominant resistance gene (pm8) was identified and assigned to chromosome 4c. in b ...201424526453
fusarium damage in cereal grains from western canada. 1. phylogenetic analysis of moniliformin-producing fusarium species and their natural occurrence in mycotoxin-contaminated wheat, oats, and rye.harvest samples of common wheat (triticum aestivum), oats (avena sativa), and rye (secale cereale) from producers in western canada were analyzed for fungal infection by toxigenic fusarium species and contamination by trichothecenes and moniliformin (mon). fusarium graminearum and f. avenaceum were the two most frequently isolated species from samples of rye and wheat collected in 2010. f. poae and f. sporotrichioides were more commonly detected in randomly selected oat seeds. other toxigenic fu ...201323683177
phytotoxicity of secondary metabolites isolated from flourensia oolepis s.f.blake.the aim of this study was to isolate the active principles of flourensia oolepis s.f.blake (asteraceae), which completely inhibited the germination of raphanus sativus seeds at 10 mg/ml. flavanone pinocembrin and sesquiterpene ilicol, were isolated by bioassay-guided fractionation. they were active both against monocot and dicot seeds. pinocembrin was the most active compound, with an ic50 (germination) value of 0.24, 3.40, 3.28, and 3.55 mm against panicum miliaceum, avena sativa, lactuca sativ ...201323847074
adaptation to acidic soil is achieved by increased numbers of cis-acting elements regulating almt1 expression in holcus lanatus.yorkshire fog (holcus lanatus), which belongs to the poaceae family and is a close relative of the agronomic crop oat (avena sativa), is a widely adaptable grass species that is able to grow on highly acidic soils with high levels of al, but the mechanism underlying the high al tolerance is unknown. here, we characterized two accessions of h. lanatus collected from an acid plot (soil ph 3.6, hl-a) and a neutral plot (ph 7.1, hl-n) in terms of al tolerance, organic acid anion secretion and relate ...201323773148
genetic transformation of major cereal crops.of the more than 50,000 edible plant species in the world, at least 10,000 species are cereal grains. three major cereal crops, rice (oryza sativa), maize (zea mays), and wheat (triticum sp.), provide two-thirds of the world's food energy intake. although crop yields have improved tremendously thanks to technological advances in the past 50 years, population increases and climate changes continue to threaten the sustainability of current crop productions. whereas conventional and marker-assisted ...201324166432
fluorescence imaging-based high-throughput screening of fast- and slow-cycling lov proteins.light-oxygen-voltage (lov) domains function as blue light-inducible molecular switches. the photosensory lov domains derived from plants and fungi have provided an indispensable tool for optogenetics. here we develop a high-throughput screening system to efficiently improve switch-off kinetics of lov domains. the present system is based on fluorescence imaging of thermal reversion of a flavin cofactor bound to lov domains. we conducted multi site-directed random mutagenesis of seven amino acid r ...201324367542
regulatory mechanism of the light-activable allosteric switch lov-tap for the control of dna binding: a computer simulation study.the spatio-temporal control of gene expression is fundamental to elucidate cell proliferation and deregulation phenomena in living systems. novel approaches based on light-sensitive multiprotein complexes have recently been devised, showing promising perspectives for the noninvasive and reversible modulation of the dna-transcriptional activity in vivo. this has lately been demonstrated in a striking way through the generation of the artificial protein construct light-oxygen-voltage (lov)-tryptop ...201323042418
primary photochemistry of the dark- and light-adapted states of the ytva protein from bacillus subtilis.the primary (100 fs to 10 ns) and secondary (10 ns to 100 μs) photodynamics in the type ii light-oxygen-voltage (lov) domain from the blue light ytva photoreceptor extracted from bacillus subtilis were explored with transient absorption spectroscopy. the photodynamics of full-length ytva were characterized after femtosecond 400 nm excitation of both the dark-adapted d447 state and the light-adapted s390 state. the s390 state relaxes on a 43 min time scale at room temperature back into d447, whic ...201324171435
coiled-coil dimerization of the lov2 domain of the blue-light photoreceptor phototropin 1 from arabidopsis thaliana.a key role in signal transduction and dimerization mediated by per-arnt-sim (pas) domains is played by α-helical linkers that flank the structurally similar α/β cores of these domains. however, crystal-packing forces and the different construct lengths and sequences of the pas domains influence the final length and orientation of the linkers relative to the core and create uncertainty in the exact mechanism of the linker function. thus, structural characterization and comparison of the linkers w ...201324316821
feed consumption, diet digestibility and mineral utilization in captive blackbuck (antelope cervicapra) fed different levels of concentrates.a feeding trial was conducted to know the level of concentrates in the diet of antelope cervicapra at which diet digestibility and mineral utilization were optimum. fifteen blackbucks (25-33 kg bw) were distributed into three groups of five each. fresh oat (avena sativa) and berseem (trifolium alexandrinum) fodders were offered ad libitum to all the animals. in addition, animals in groups ii and iii received concentrate mixture at the rate of 0.5 and 1% of bw, whereas animals in group i received ...201322093070
molecular cloning of the apoptosis-related calcium-binding protein asalg-2 in avena sativa.victorin, the host-selective toxin produced by the fungus cochliobolus victoriae, induces programmed cell death (pcd) in victorin-sensitive oat lines with characteristic features of animal apoptosis, such as mitochondrial permeability transition, chromatin condensation, nuclear dna laddering and rrna/mrna degradation. in this study, we characterized a calcium-binding protein, namely asalg-2, which might have a role in the victorin-induced pcd. asalg-2 is homologous to the apoptosis-linked gene a ...201323083467
avena sativa (oat), a potential neutraceutical and therapeutic agent: an overview.the aim of the present review article is to summarize the available information related to the availability, production, chemical composition, pharmacological activity, and traditional uses of avena sativa to highlight its potential to contribute to human health. oats are now cultivated worldwide and form an important dietary staple for the people in number of countries. several varieties of oats are available. it is a rich source of protein, contains a number of important minerals, lipids, β-gl ...201323072529
effect of treated and untreated domestic wastewater on seed germination, seedling growth and amylase and lipase activities in avena sativa l.oats (avena sativa l.) are a potential economically viable source of lipids and starch for use in foods. the aim of this study was to determine the effect of treated and untreated urban wastewater on seed germination, growth parameters and lipase and amylase activities in a. sativa.201323070708
the myth of nitrogen fertilization for soil carbon sequestration.intensive use of n fertilizers in modern agriculture is motivated by the economic value of high grain yields and is generally perceived to sequester soil organic c by increasing the input of crop residues. this perception is at odds with a century of soil organic c data reported herein for the morrow plots, the world's oldest experimental site under continuous corn (zea mays l.). after 40 to 50 yr of synthetic fertilization that exceeded grain n removal by 60 to 190%, a net decline occurred in s ...201317965385
copper speciation and isotopic fractionation in plants: uptake and translocation mechanisms.the fractionation of stable copper (cu) isotopes during uptake into plant roots and translocation to shoots can provide information on cu acquisition mechanisms. isotope fractionation ((65) cu/(63) cu) and intact tissue speciation techniques (x-ray absorption spectroscopy, xas) were used to examine the uptake, translocation and speciation of cu in strategy i (tomato-solanum lycopersicum) and strategy ii (oat-avena sativa) plant species. plants were grown in controlled solution cultures, under va ...201323600562
cover crop mulch and weed management influence arthropod communities in strip-tilled cabbage.cover crop mulch and weeds create habitat complexity in agricultural fields that may influence arthropods. under strip-tillage systems, planting rows are tilled and preestablished cover crops can remain between rows. in field experiments conducted in michigan in 2010 and 2011, a preestablished oat (avena sativa l.) cover crop was allowed to grow between rows of strip-tilled cabbage and killed at 0, 9-14, or 21-27 d after transplanting (dat). the effects of herbicide intensity and oat kill date o ...201323575020
sustained accurate recording of intracellular acidification in living tissues with a photo-controllable bioluminescent protein.regulation of an intracellular acidic environment plays a pivotal role in biological processes and functions. however, spatiotemporal analysis of the acidification in complex tissues of living subjects persists as an important challenge. we developed a photo-inactivatable bioluminescent indicator, based on a combination of luciferase-fragment complementation and a photoreaction of a light, oxygen, and voltage domain from avena sativa phototropin1 (lov2), to visualize temporally dynamic acidifica ...201323690604
an optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility.we have developed an optogenetic technique for the activation of diaphanous-related formins. our approach is based on fusion of the light-oxygen-voltage 2 domain of avena sativa phototrophin1 to an isolated diaphanous autoregulatory domain from mdia1. this "caged" diaphanous auto-regulatory domain was inactive in the dark but in the presence of blue light rapidly activated endogenous diaphanous-related formins. using an f-actin reporter, we observed filopodia and lamellipodia formation as well a ...201323677607
effect of maturity at harvest on yield, chemical composition, and in situ degradability for annual cereals used for swath grazing.the objective of this study was to determine how harvest maturity of whole-crop cereals commonly used in swath grazing systems in western canada affects yield, chemical composition, and in situ digestibility. we hypothesized that the increase in yield with advancing maturity would not offset the decline in digestibility and, thus, the yield of effectively degradable dm (eddm) would decline with advanced stages of maturity. four replicate plots of barley (hordeum vulgare l.; cv. cdc cowboy), mill ...201323658356
biosorption and bioreduction of copper from different copper compounds in aqueous solution.high copper concentration is toxic for living organisms including humans. biosorption is a bioremediation technique that can remove copper and other pollutants from aqueous medium and soils, consequently cleaning the environment. the aim of this study was, therefore, to investigate the influence of different copper compounds (cu(ii) as cucl2; cu(ii) as cuso4; and cu(i) as cucl) on copper bioreduction and biosorption using four copper-resistant bacteria isolated from the rhizosphere of two plants ...201323417495
effect of oat consumption on lipid profiles in hypercholesterolemic adults.hypercholesterolemia is a strong risk factor in cardiovascular disease. oat (avena sativa l.) beta-glucan, the soluble fiber in oat, has been known to reduce blood cholesterol levels considerably. however, the effect of oat soluble fiber in the thai population is unknown.201324851570
optimized utilization of the co-products from bioethanol processing and oat grain: effect of blending on biochemical, biodegradation, and nutritional profiles.the objective of this study was to (1) optimize the utilization of wheat-based dried distillers grains with soluble (wddgs) by blending with oat ( avena sativa l.) grain as an alternative feed for beef cattle when the barley price is high and (2) investigate the effect of blending on biochemical, biodegradation, and nutritional profiles. oat grains were blended with wddgs produced in western canada at different levels (4:0, 3:1, 2:2, and 1:3 on %dm basis in two batches, denoted o0, o25, o50, and ...201324195597
characterization of oats (avena sativa l.) cultivars using machine vision.machine vision or image analysis is an important tool in the study of morphology of any materials. this technique has been used successfully to differentiate the eleven oats cultivars based on morphological characters. the geometry of seeds was measured through image analyzer and the variation was observed and recorded. from the recorded data, the cluster analysis was carried out and it revealed that the cultivars could be grouped into two main clusters based on similarity in the measured parame ...201324506019
investigation of the roles of fascioliasis and food allergy in intrahepatic eosinophilic proliferative pylephlebitis in japanese black cattle.intrahepatic eosinophilic proliferative pylephlebitis (epp) in japanese black (jb) cattle generally has been considered to be an atypical form of fascioliasis. however, there are many cases of epp in which no fasciola spp. have been detected in the livers of affected cattle. the aims of this study were to ascertain the relationship between epp and hepatic fascioliasis and to investigate the role of food allergy in the disease. histologically, epp lesions were characterised by severe endothelial ...201323131780
identification of high β-glucan oat lines and localization and chemical characterization of their seed kernel β-glucans.oat, (avena sativa) is an excellent source of mixed linkage β-glucans ((1→3)(1→4)-β-d-glucan), a dietary fibre with cholesterol lowering properties. using a mutagenized oat-population we screened 1700 different lines and identified ten lines that displayed β-glucan levels above 6.7% and 10 below 3.6%.the extreme values were 1.8% and 7.5%. we chose six lines with an increased- and four lines with a reduced β-glucan content for further study. by longitudinal- and cross-sections of seeds it was sho ...201323199994
effect of concentrate supplementation on feed consumption, nutrient utilization and blood metabolite profile in captive spotted deer (axis axis) fed oat (avena sativa) and berseem (trifolium alexandrinum) fodders based diet.this experiment was conducted to determine the optimum level of a maize-soybean meal-wheat bran concentrate supplement fed to captive spotted deer fed an oat and berseem fodder-based diet. twelve adult spotted deer [64-76 kg body weight (bw)] were distributed into three groups of four each and were housed individually. a diet consisting of 5 kg of oat fodder and 5.5 kg of berseem fodder was offered to each one of the experimental animals. the animal in group i received no supplementary concentra ...201323349033
histological analysis and 3d reconstruction of winter cereal crowns recovering from freezing: a unique response in oat (avena sativa l.).the crown is the below ground portion of the stem of a grass which contains meristematic cells that give rise to new shoots and roots following winter. to better understand mechanisms of survival from freezing, a histological analysis was performed on rye, wheat, barley and oat plants that had been frozen, thawed and allowed to resume growth under controlled conditions. extensive tissue disruption and abnormal cell structure was noticed in the center of the crown of all 4 species with relatively ...201323341944
toxicity assessment for petroleum-contaminated soil using terrestrial invertebrates and plant bioassays.the assessment of soil quality after a chemical or oil spill and/or remediation effort may be measured by evaluating the toxicity of soil organisms. to enhance our understanding of the soil quality resulting from laboratory and oil field spill remediation, we assessed toxicity levels by using earthworms and springtails testing and plant growth experiments. total petroleum hydrocarbons (tph)-contaminated soil samples were collected from an oilfield in sfax, tunisia. two types of bioassays were pe ...201322773148
study on the sensitivity of three oat varieties to the saddle gall midge, haplodiplosis margina ta (von roser) (diptera: cecidomyiidae).the saddle gall midge, haplodiplosis marginota (von roser, 1840) is a univoltine pest of cereals which occurs in europe. the larvae feed on stems and attractive saddle-shaped depressions, driving to important yield losses when the galls are numerous. after 40 years without any reporting, large populations of h. marginata and important damage have been observed since 2010 in wheat crops in belgium, especially in the flemish polders where clay soils and intensive farming of cereals favour heavy in ...201325145247
ecotoxicity of climbazole, a fungicide contained in antidandruff shampoo.emerging pollutants such as personal care products can reach the environment via effluents from wastewater treatment plants (wwtps) and digested sludge. only recently, the antidandruff agent and antimycotic climbazole was detected for the first time in a wwtp effluent with concentrations up to 0.5 µg/l. climbazole acts as a c14-demethylase inhibitor (dmi) fungicide and thus has a high efficacy against fungi, but knowledge of its potential environmental impact is lacking. therefore, the aim of th ...201323982925
differential speed of activation in antioxidant system in three oat genotypes.the objective of this study was to evaluate whether the oxidative stress caused by aluminum (al) toxicity is a symptom that can trigger root growth inhibition in oat genotype seedlings. oat seedlings were grown in a nutrient solution (ph 4.0) with 0 and 370 μm al. at 12, 24, and 36 h after al addition, growth (root length) and biochemical parameters (catalase - cat, ascorbate peroxidase - apx, and superoxide dismutase - sod activities, lipid peroxidation, ascorbic acid (asa) and non-protein thio ...201323998202
assessment of tri- and hexavalent chromium phytotoxicity on oats (avena sativa l.) biomass and content of nitrogen compounds.the purpose of this study was to determine the effect of soil contamination with tri- and hexavalent chromium and soil application of compost, zeolite, and cao on the mass of oats and content of nitrogen compounds in different organs of oats. the oats mass and content of nitrogen compounds in the crop depended on the type and dose of chromium and alleviating substances incorporated to soil. in the series without neutralizing substances, cr(vi), unlike cr(iii), had a negative effect on the growth ...201323853394
phytotoxicity of new furan-derived aminophosphonic acids, n-aryl furaldimines and 5-nitrofuraldimine.the aim of this work was to synthesize selected furaldimines and their aminophosphonic derivatives and evaluation the phytotoxicity of new obtained products according to oecd 208 guideline. four schiff bases, n-furfurylidene-p-anisidine (1a), n-furfurylidene-p-toluidine (1b), n-furfurylidene-benzhydrylamine (1c), and n-(2-nitrofurfurylidene)-p-toluidine (1d) were synthesized and three new furan-derived n-substituted aminomethylphosphonic acids, namely: 2-furyl n-(p-methoxyphenyl)-aminomethylphos ...201323869973
[acute massive pulmonary embolism in a patient using clavis panax].in recent years, the use of herbal combinations, plant extracts or food supplements has increased in our country and all over the world. however, there is not enough data to determine the effective doses of these substances in the composition of herbal preparations, or their effects on metabolism and drug interactions. with the widespread use of herbal combinations, life-threatening side effects and clinical manifestations that arise from them have been reported. herein we present a case with ac ...201323760126
snp discovery and chromosome anchoring provide the first physically-anchored hexaploid oat map and reveal synteny with model species.a physically anchored consensus map is foundational to modern genomics research; however, construction of such a map in oat (avena sativa l., 2n = 6x = 42) has been hindered by the size and complexity of the genome, the scarcity of robust molecular markers, and the lack of aneuploid stocks. resources developed in this study include a modified snp discovery method for complex genomes, a diverse set of oat snp markers, and a novel chromosome-deficient snp anchoring strategy. these resources were a ...201323533580
signaling mechanisms of lov domains: new insights from molecular dynamics studies.phototropins are one of several classes of photoreceptors used by plants and algae to respond to light. these proteins contain flavin-binding lov (light-oxygen-voltage) domains that form covalent cysteine-flavin adducts upon exposure to blue light, leading to the enhancement of phototropin kinase activity. several lines of evidence suggest that adduct formation in the phototropin lov2 domains leads to the dissociation of an alpha helix (jα) from these domains as part of the light-induced activat ...201323407663
investigating models of protein function and allostery with a widespread mutational analysis of a light-activated protein.to investigate the relationship between a protein's sequence and its biophysical properties, we studied the effects of more than 100 mutations in avena sativa light-oxygen-voltage domain 2, a model protein of the per-arnt-sim family. the a. sativa light-oxygen-voltage domain 2 undergoes a photocycle with a conformational change involving the unfolding of the terminal helices. whereas selection studies typically search for winners in a large population and fail to characterize many sites, we char ...201323972854
linkage mapping and identification of qtl affecting deoxynivalenol (don) content (fusarium resistance) in oats (avena sativa l.).mycotoxins caused by fusarium spp. is a major concern on food and feed safety in oats, although fusarium head blight (fhb) is often less apparent than in other small grain cereals. breeding resistant cultivars is an economic and environment-friendly way to reduce toxin content, either by the identification of resistance qtl or phenotypic evaluation. both are little explored in oats. a recombinant-inbred line population, hurdal × z595-7 (hz595, with 184 lines), was used for qtl mapping and was ph ...201323959525
rapid analysis of avenacosides in grain and husks of oats by uplc-tq-ms.avenacosides a (aa) and b (ab) as well as 26-desglucoavenacoside a (26daa) were quantified in oats using rapid and sensitive method utilising uplc-tq-ms. in the grain, aa and ab were revealed as the primary saponins, whereas in the husks, daa was predominant. inconsistent with the published data, observed concentrations of aa and ab in the grain were very similar. presumably, this is due to higher sensitivity and better selectivity of the mass spectrometry-based quantification method, thus allow ...201323870961
nutrient intake, digestibility, and blood metabolites of goats fed diets containing processed jatropha meal.this study was conducted to record the ideal source and level of alkali treatment to treat jatropha meal (jm) and to determine the effect of inclusion of variously processed jm (pjm) on nutrient intake, digestibility, blood metabolites and hormonal status in goats. the jm was treated with 10 g/kg sodium chloride and 5 g/kg calcium hydroxide. the content of phorbol ester and hemagglutination (ha) activity of jm and pjm were assessed. a feeding trial for 90 days was conducted in short-haired multi ...201323526123
dynamics of the chemical composition and productivity of composts for the cultivation of agaricus bisporus strains.two compost formulations based on oat straw (avena sativa) and brachiaria (brachiaria sp.) were tested for the cultivation of three agaricus bisporus strains (abi-07/06, abi-05/03, and pb-1). the experimental design was a 2 × 3 factorial scheme (composts × strains) with 6 treatments and 8 repetitions (boxes containing 12 kg of compost). the chemical characterization of the compost (humidity, organic matter, carbon, nitrogen, ph, raw protein, ethereal extract, fibers, ash, cellulose, hemicellulos ...201324688503
endogenous nitrogen excretion by red kangaroos (macropus rufus): effects of animal age and forage quality.red kangaroos (macropus rufus) are large (>20 kg) herbivorous marsupials common to arid and semiarid australia. the population dynamics of red kangaroos are linked with environmental factors, operating largely through juvenile survival. a crucial period is the young-at-foot (yaf) stage, when juveniles have permanently left the mother's pouch but still take milk from a teat in the pouch. forage quantity and quality have been implicated in drought-related mortalities of juvenile kangaroos. here we ...201316555200
interactions between chromophore and protein in phytochrome identified by novel oxa-, thia- and carba-chromophores.six new bilin chromophores of the plant photoreceptor phytochrome have been synthesized, carrying at the photoisomerizing ring d an oxygen or a sulfur atom or a methylene group instead of the pyrrole nitrogen atom. these furanone-, thiophenone- or cyclopentenone-containing compounds bound covalently to the recombinant apophytochrome phya of avena sativa. the novel chromoproteins showed hypsochromically shifted absorption spectra with respect to native phytochrome and a strongly diminished photoc ...201318363618
oscillations of acetylcholine in oat seedlings.using gas chromatography it was shown that acetylcholine (ach) was present in both etiolated and green oat (avena sativa l. cv. diadem) seedlings. in etiolated seedlings the ach level was low, but increased rapidly during exposure to sunlight and red light (rl). the stimulative influence of rl was reversed by far-red light (frl). the rl- and frl- changes in ach level were correlated to changes in acetylcholinesterase (ache) localization. using karnovsky's method, it was found that in the etiolat ...20132350987
safety and efficacy of personal care products containing colloidal oatmeal.colloidal oatmeal is a natural ingredient used in the formulation of a range of personal care products for relief of skin dryness and itchiness. it is also used as an adjunctive product in atopic dermatitis. the safety of personal care products used on vulnerable skin is of particular importance and the risk of developing further skin irritations and/or allergies should be minimized.201223204849
targeting sources of drought tolerance within an avena spp. collection through multivariate approaches.in this study, we find and characterize the sources of tolerance to drought amongst an oat (avena sativa l.) germplasm collection of 174 landraces and cultivars. we used multivariate analysis, non-supervised principal component analyses (pca) and supervised discriminant function analyses (dfa) to suggest the key mechanism/s responsible for coping with drought stress. following initial assessment of drought symptoms and area under the drought progress curve, a subset of 14 accessions were selecte ...201222824964
chronic effects of a wild green oat extract supplementation on cognitive performance in older adults: a randomised, double-blind, placebo-controlled, crossover trial.preliminary evaluation of a wild green oat extract (wgoe) (neuravena(®) elfa(®)955, frutarom, switzerland) revealed an acute cognitive benefit of supplementation. this study investigated whether regular daily wgoe supplementation would result in sustained cognitive improvements.201222690320
individual maize chromosomes in the c(3) plant oat can increase bundle sheath cell size and vein density.c(4) photosynthesis has evolved in at least 66 lineages within the angiosperms and involves alterations to the biochemistry, cell biology, and development of leaves. the characteristic "kranz" anatomy of most c(4) leaves was discovered in the 1890s, but the genetic basis of these traits remains poorly defined. oat × maize addition lines allow the effects of individual maize (zea mays; c(4)) chromosomes to be investigated in an oat (avena sativa; c(3)) genetic background. here, we have determined ...201222675083
integrated approach to assessing the effects of soils polluted with heavy metals on a plant population.this study addresses the effects of soil polluted with more than one heavy metal in a grass species. a 16-week bioassay with avena sativa l. was conducted in microcosms using soils from two abandoned mines in central spain that contained levels above world health organization (who) reference limits for soils of more than three heavy metals. pollution effects were examined at cell, tissue, organ, plant and population levels. for this purpose, dry weight, maximum height and number of leaves were d ...201222623112
effect of feeding piglets with different extruded and nonextruded cereals on the gut mucosa and microbiota during the first postweaning week.two trials were conducted to evaluate the effect of different cereals in piglet diets on the jejunal mucosa and the ileal and cecal microbiota during the first postweaning days. in trial 1, 48 newly weaned pigs (7.95 kg bw; 26 d of age) were individually housed and distributed among 3 experimental diets containing white rice (oryza sativa), naked oats (avena sativa), or barley (hordeum vulgare) as the cereal source. at the start of the trial (weaning; day 0), 12 piglets were slaughtered and samp ...201223365267
[a sudden rise in inr due to combination of tribulus terrestris, avena sativa, and panax ginseng (clavis panax)].warfarin sodium is an antithrombin agent used in patients with prosthetic valve and atrial fibrillation. however, there are many factors that can change the effectiveness of the drug. today, herbal mixtures promoted through targeted print and visual media can lead to sudden activity changes in patients using warfarin. in this case report we will present two cases with a sudden rise in inr due to using combination of tribulus terrestris, avena sativa and panax ginseng (panax clavis). two patients ...201222864323
controlling the dna cleavage activity of light-inducible chimeric endonucleases by bidirectional photoactivation.a functional coupling of photosensory domains derived from photoreceptors to effector proteins is a promising strategy for engineering novel photoresponsive proteins in optogenetics. here, we have fused the light-sensitive lov2 domain from avena sativa phototropin1 to the restriction enzyme pvuii to generate a genetically encoded, light-controllable endonuclease. by analyzing several lov-pvuii fusion enzymes, variants were obtained that show a 3-fold difference in dna cleavage activity, when ill ...201222559722
effect of feeding different cereal-based diets on the performance and gut health of weaned piglets with or without previous access to creep feed during lactation.a trial was conducted to evaluate the effect of different cereals on the performance, gut mucosa, and microbiota of weanling pigs with or without previous access to creep feed during lactation. a total of 108 newly weaned pigs (7.4 kg bw; 26 d of age; half with and half without creep feed) were used. piglets were distributed by bw into 36 pens according to a 2 × 6 factorial arrangement of treatments with previous access to creep feed (with or without) and cereal source in the experimental diet [ ...201223365275
lactose in diet influences the degradation of mixed linked β(1-3;1-4)-d-glucan in the small intestine of pigs.the objective was to study the cause of variation in digestibility of mixed linked β(1-3;1-4)-d-glucan (β-glucan) in the small intestine of growing pigs. the β-glucan is an important cell wall [dietary fiber (df)] component of the endosperm of barley (hordeum vulgare) and oats (avena sativa). the digestibility of β-glucan in the small intestine from both cereals is among the highest of all df components, but in 1 study with oat-based diets it was lower (p < 0.001) than in other studies. in this ...201223365304
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