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antidiabetic effects of tea.diabetes mellitus (dm) is a chronic endocrine disease resulted from insulin secretory defect or insulin resistance and it is a leading cause of death around the world. the care of dm patients consumes a huge budget due to the high frequency of consultations and long hospitalizations, making dm a serious threat to both human health and global economies. tea contains abundant polyphenols and caffeine which showed antidiabetic activity, so the development of antidiabetic medications from tea and it ...201728531120
metabolic phenotyping of various tea (camellia sinensis l.) cultivars and understanding of their intrinsic metabolism.recently, we selected three tea (camellia sinensis) cultivars that are rich in taste, epigallocatechin-3-o-gallate (egcg) and epigallocatechin-3-o-(3-o-methyl)-gallate (egcg3″me) and then cultivated them through asexual propagation by cutting in the same region. in the present study, proton nuclear magnetic resonance (1h nmr)-based metabolomics was applied to characterize the metabotype and to understand the metabolic mechanism of these tea cultivars including wild type tea. of the tea leaf meta ...201728530581
characterization of white tea metabolome: comparison against green and black tea by a nontargeted metabolomics approach.white tea is considered the least processed form of tea and is reported to have a series of potent bioactivities, such as antioxidant, anti-inflammatory, anti-mutagenic, and anti-cancer activities. however, the chemical composition of white tea and the dynamic changes of the metabolites during the manufacturing process are far from clear. in this study, we applied a nontargeted metabolomics approach based on ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass ...201728528106
functional characterizations of β-glucosidases involved in aroma compound formation in tea (camellia sinensis).tea (camellia sinensis) aroma is an important factor affecting tea quality. many tea aroma compounds are present as glycosidically conjugated forms in tea leaves, and can be hydrolyzed by β-glucosidase (β-glu) and β-primeverosidase to release free tea aromas. β-primeverosidase has been identified and functionally characterized, while β-glu has not been identified in tea leaves. in the present study, we established a yeast expression system to recombine csgh1bg1, csgh3bg1, and csgh5bg1, which bel ...201728528101
proteomic analysis of tea plants (camellia sinensis) with purple young shoots during leaf development.tea products made from purple leaves are highly preferred by consumers due to the health benefits. this study developed a proteome reference map related to color changes during leaf growth in tea (camellia sinensis) plant with purple young shoots using two-dimensional electrophoresis (2-de). forty-six differentially expressed proteins were detected in the gel and successfully identified by using maldi-tof/tof-ms. the pronounced changes in the proteomic profile between tender purple leaves (tpl) ...201728520776
expression of key structural genes of the phenylpropanoid pathway associated with catechin epimerization in tea cultivars.catechin epimerization is an important factor affecting tea catechin compositions and thereby tea quality. however, a lack of tea germplasms with high non-epicatechins limits relative research. here, a tea cultivar y510 with high non-epicatechins was firstly reported and used for catechin and rna sequencing (rna-seq) analysis. results showed that the (-)-gallocatechin gallate and (+)-catechin (c) contents in y510 were at least 136 and 6 times higher than those in fudingdabaicha and 0306i, but th ...201728515736
characterization of l-theanine excitatory actions on hippocampal neurons: toward the generation of novel n-methyl-d-aspartate receptor modulators based on its backbone.l-theanine (or l-γ-n-ethyl-glutamine) is the major amino acid found in camellia sinensis. it has received much attention because of its pleiotropic physiological and pharmacological activities leading to health benefits in humans, especially. we describe here a new, easy, efficient, and environmentally friendly chemical synthesis of l-theanine and l-γ-n-propyl-gln and their corresponding d-isomers. l-theanine, and its derivatives obtained so far, exhibited partial coagonistic action at n-methyl- ...201728511005
short-term effects of green tea chronotherapy on the metabolic homeostasis of mice on different diets.biological rhythms can be defined as changes in physiological or behavioral variables that repeat at certain time intervals. rhythms that last approximately 24 h are referred to as circadian rhythms. modern lifestyles have drastically affected human habits, as well as the population's eating habits. these changes have generated an epidemic of metabolic syndromes, such as obesity and diabetes. in an attempt to combat obesity, populations have attempted to use many different herbal remedies and pl ...201728510251
camellia sinensis increased apoptosis on u2os osteosarcoma cells and wound healing potential on nih3t3 fibroblast cells.camellia sinensis (cs) is a plant which is rich in polyphenols and has antioxidant, antiinflammatory, antimutagenic, anticarcinogenic and antibacterial activities. in this study, two different methanol extracts (cs-i and cs-ii) were prepared from the leaf of c. sinensis in order to investigate the wound healing and anticancer activities. total phenolic content and antioxidant activity of the extracts were determined. wound healing effects of cs extracts were evaluated by using masson's trichrome ...201728509991
quantitative succinyl-proteome profiling of camellia sinensis cv. 'anji baicha' during periodic albinism.lysine succinylation is a novel dynamic and evolutionarily conserved post-translational modification (ptm) that regulates various biological processes. 'anji baicha' is an albino tea variety that exhibits temperature-based variability of leaf colour and amino acid concentrations. however, the mechanism underlying albinism in 'anji baicha' has not been investigated at the level of succinylation. here, we identify 3530 lysine succinylation sites mapped to 2132 proteins in 'anji baicha', representi ...201728500349
nickel nanoparticle synthesis using camellia sinensis as reducing and capping agent: growth mechanism and photo-catalytic activity evaluation.recently, the biosynthesis of nanoparticle attracted the attention of scientific community due to its simplicity, ease and eco-friendly nature. in the present study, camellia sinensis (c. sinensis) leaves extract was employed for the synthesis of nickel nanoparticles (ninps). the fabricated ninps were characterized by scanning electron microscopy (sem) and energy-dispersive x-ray (edx) and x-ray diffraction techniques. the photocatalytic activity (pca) was evaluated by degrading crystal violet ( ...201728495625
artemisinin-luminol chemiluminescence for forensic bloodstain detection using a smart phone as a detector.forensic luminol chemiluminescence test is one of the most sensitive and popular methods for the determination of latent bloodstains. it mainly uses hydrogen peroxide or sodium perborate as coreactants. the easy decomposition of hydrogen peroxide and sodium perborate in the presence of many ions significantly affects the selectivity. artemisinin is a natural peroxide that is quite stable in the presence of common ions. in the present study, artemisinin has been exploited for the forensic bloodst ...201728492080
transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (camellia sinensis).applied nitrogen (n) fertilizer significantly increases the leaf yield. however, most n is not utilized by the plant, negatively impacting the environment. to date, little is known regarding n utilization genes and mechanisms in the leaf production. to understand this, we investigated transcriptomes using rna-seq and amino acid levels with n treatment in tea (camellia sinensis), the most popular beverage crop. we identified 196 and 29 common differentially expressed genes in roots and leaves, re ...201728490757
effects of dietary green tea (camellia sinensis l.) supplementation on growth performance, lipid metabolism, and antioxidant status in a sturgeon hybrid of sterlet (huso huso ♂ × acipenser ruthenus ♀) fed oxidized fish oil.lipid content of diet is very susceptible to oxidation, especially when stored for a long time, so for evaluating protective effects of green tea in fish received oxidized oil, this study was done. lipid content of diet was replaced by oxidized fish oil (ofo) in 0, 50, and 100%. green tea extract (gte) was added to diet in three levels, 0, 5, and 100 mg/kg giving a total of nine experimental diets. two hundred and seventy sturgeon hybrid of sterlet (huso huso ♀ × acipenser ruthenus ♂) with initi ...201728488192
nitric oxide mediates brassinosteroid-induced flavonoid biosynthesis in camellia sinensis l.flavonoids are one of the key secondary metabolites determining the quality of tea. although exogenous brassinosteroid (br), a steroidal plant hormone, can stimulate polyphenol biosynthesis in tea plants (camellia sinensis l.), the relevance of endogenous br in flavonoid accumulation and the underlying mechanisms remain largely unknown. here we show that br enhances flavonoid concentration in tea leaves by inducing an increase in the endogenous concentration of nitric oxide (no). notably, exogen ...201728482335
probable benefits of green tea with genetic implications.tea is produced from the camellia sinensis plant and can generally be divided into categories based on how they are processed. in general, green tea that is unfermented c. sinensis has been considered superior to black tea in health benefits. it contains a unique set of catechins that possess biological activity as antioxidant, anti-inflammatory and antiproliferative, which is potentially significant to the prevention and treatment of various forms of diseases. oral cavity oxidative stress and i ...201728479696
oak kombucha protects against oxidative stress and inflammatory processes.black tea infusion is the common substrate for preparing kombucha; however other sources such as oak leaves infusions can be used for the same purpose. almost any white oak species have been used for medicinal applications by some ethnic groups in mexico and could be also suitable for preparing kombucha analogues from oak (kao). the objective of this research was to investigate the antioxidant activity and anti-inflammatory effects of kao by examining its modulation ability on macrophage-derived ...201728476604
the tea tree genome provides insights into tea flavor and independent evolution of caffeine biosynthesis.tea is the world's oldest and most popular caffeine-containing beverage with immense economic, medicinal, and cultural importance. here, we present the first high-quality nucleotide sequence of the repeat-rich (80.9%), 3.02-gb genome of the cultivated tea tree camellia sinensis. we show that an extraordinarily large genome size of tea tree is resulted from the slow, steady, and long-term amplification of a few ltr retrotransposon families. in addition to a recent whole-genome duplication event, ...201728473262
breast cancer chemopreventive and chemotherapeutic effects of camellia sinensis (green tea): an updated review.camellia sinensis belongs to the plant family of theaceae, native to east asia, the indian subcontinent and southeast asia, but naturalized in many parts of the world. the aim of this study was to overview its anti-breast cancer chemopreventive and chemotherapeutic effects. this review article is aimed to overview breast cancer chemopreventive and chemotherapeutic effects of camellia sinensis (green tea).201728465816
supplementation with different teas from camellia sinensis prevents memory deficits and hippocampus oxidative stress in ischemia-reperfusion.memory and cognition impairments resultant of ischemic stroke could be minimized or avoided by antioxidant supplementation. in this regard, the neuroprotective potential of green tea from camellia sinensis has been investigated. however, there is a lack of information regarding the neuroprotective potential of others teas processed from the camellia sinensis. here we investigate the neuroprotective role of green, red, white and black tea on memory deficits and brain oxidative stress in a model o ...201728465087
comprehensive transcriptome analyses reveal differential gene expression profiles of camellia sinensis axillary buds at para-, endo-, ecodormancy, and bud flush stages.winter dormancy is an important biological feature for tea plant to survive cold winters, and it also affects the economic output of tea plant, one of the few woody plants in the world whose leaves are harvested and one of the few non-conifer evergreen species with characterized dormancies. to discover the bud dormancy regulation mechanism of tea plant in winter, we analyzed the global gene expression profiles of axillary buds at the paradormancy, endodormancy, ecodormancy, and bud flush stages ...201728458678
corneal staining and hot black tea compresses.warm compresses are widely touted as an effective treatment for ocular surface disorders. black tea compresses are a common household remedy, although there is no evidence in the medical literature proving their effect and their use may lead to harmful side effects.201728457091
heterologous expression of three camellia sinensis small heat shock protein genes confers temperature stress tolerance in yeast and arabidopsis thaliana.cshsp17.7, cshsp18.1, and cshsp21.8 expressions are induced by heat and cold stresses, and cshsp overexpression confers tolerance to heat and cold stresses in transgenic pichia pastoris and arabidopsis thaliana. small heat shock proteins (shsps) are crucial for protecting plants against biotic and abiotic stresses, especially heat stress. however, knowledge concerning the functions of camellia sinensis shsp in heat and cold stresses remains poorly understood. in this study, three c. sinensis shs ...201728455764
reliable reference genes for normalization of gene expression data in tea plants (camellia sinensis) exposed to metal stresses.tea plants [camellia sinensis (l.) o. kuntze] are an important leaf-type crop that are widely used for the production of non-alcoholic beverages in the world. exposure to excessive amounts of heavy metals adversely affects the quality and yield of tea leaves. to analyze the molecular responses of tea plants to heavy metals, a reliable quantification of gene expression is important and of major importance herein is the normalization of the measured expression levels for the target genes. ideally, ...201728453515
comparison of metabolomics approaches for evaluating the variability of complex botanical preparations: green tea (camellia sinensis) as a case study.a challenge that must be addressed when conducting studies with complex natural products is how to evaluate their complexity and variability. traditional methods of quantifying a single or a small range of metabolites may not capture the full chemical complexity of multiple samples. different metabolomics approaches were evaluated to discern how they facilitated comparison of the chemical composition of commercial green tea [camellia sinensis (l.) kuntze] products, with the goal of capturing the ...201728453261
formation of (e)-nerolidol in tea (camellia sinensis) leaves exposed to multiple stresses during tea manufacturing.(e)-nerolidol is a volatile sesquiterpene that contributes to the floral aroma of teas (camellia sinensis). the unique manufacturing process for oolong tea involves multiple stresses, resulting in a high content of (e)-nerolidol, which is not known to form in tea leaves. this study aimed to determine the formation mechanism of (e)-nerolidol in tea exposed to multiple stresses during tea manufacture. c. sinensis (e)-nerolidol synthase (csnes) recombinant protein, found in the cytosol, was found t ...201728450026
l-theanine content and related gene expression: novel insights into theanine biosynthesis and hydrolysis among different tea plant (camellia sinensis l.) tissues and cultivars.l-theanine content has tissues and cultivars specificity in tea plant (camellia sinensis l.), the correlations of theanine metabolic related genes expression profiles with theanine contents were explored in this study. l-theanine contents in the bud and 1st leaf, 2nd leaf, 3rd leaf, old leaf, stem, and lateral root were determined by hplc from three c. sinensis cultivars, namely 'huangjinya', 'anjibaicha', and 'yingshuang', respectively. the theanine contents in leaves and root of 'huangjinya' w ...201728439281
targeted intracellular heat transfer in cancer therapy: assessment of asparagine-laminated gold nanoparticles in cell model of t cell leukemia.high temperatures have destructive effects on cancer cells by damaging proteins and structures within cells. gold nanoparticles (aunps) can act as drug delivery vehicles, especially for cancer therapy. due to the selective intake of asparagine molecules into malignant cells, aunps were coated with asparagine; and ccrf-cem human t-cell leukemia cells were treated with the new combination, asn-aunps, at 39 °c.201728435822
effect of black tea consumption on radial blood pulse spectrum and cognitive health.black tea consumption has been proven to improve endothelial function and to lower the risk of stroke and cognitive impairment. several effects of black tea on cardiovascular system had been surveyed. however, the black tea effect on pressure pulse spectrum remains unknown. the study was aimed to investigate the influence of black tea on radial blood pressure and pulse spectrum. fourteen healthy subjects received water and single doses of black tea (0.05g/kg) in separate weeks. the radial blood ...201728434461
theanine: the unique amino acid in the tea plant as an oral hepatoprotective agent.for thousands of years, humans have consumed tea made from leaves of camellia sinensis, first as a medicinal herb and then as a widely popular beverage. in the past 10 years, theanine, a tea-derived, unique, nonproteinic amino acid, has been extensively studied for its health benefits. recently, multiple lines of evidence have proven its beneficial effects on hepatic and immune functions. one possible mechanism for its biological activity involves the downregulation of the inflammatory response ...201728429900
regulation of anthocyanin biosynthesis in purple leaves of zijuan tea (camellia sinensis var. kitamura).plant anthocyanin biosynthesis is well understood, but the regulatory mechanism in purple foliage tea remains unclear. using isobaric tag for relative and absolute quantification (itraq), 815 differential proteins were identified in the leaves of zijuan tea, among which 20 were associated with the regulation of anthocyanin metabolism. we found that the abundances of anthocyanin synthesis-related enzymes such as chalcone synthase, chalcone isomerase, dihydroflavonol 4-reductase and anthocyanin sy ...201728422049
quality, antioxidant activity and composition of indian black teas.a comparative study was undertaken to determine the difference in quality, antioxidant activity, and mineral content of northern and southern indian tea varieties. the results showed that the antioxidant activity, theaflavin and thearubigin content, color particle size and total soluble solids influence the tea leaf and tea dust. assam leaf showed higher values for all the physico-chemical properties followed by darjeeling leaf. in addition, the mineral composition of the tea infusion was also i ...201728416877
impact of light irradiation on black tea quality during withering.black tea manufacture usually involves the processes of withering, cutting, fermentation and drying. the aim of present study was to evaluate the effect of the relationship between the quality and withering with different light sources (ultraviolet, yellow, blue, purple, orange, red, cyan, green and white) an quality attribute of tea. the results indicated that the yellow, orange and red light withering significantly improved the aroma and taste, imparting the tea a sweet flavor and a fresh and ...201728416872
spontaneously assembled nano-aggregates in clear green tea infusions from camellia ptilophylla and camellia sinensis.tea nano-aggregates spontaneously assembled in clear tea infusions are considered as the precursors of tea cream, although their molecular basis remains obscure. here, we characterized nano-aggregates in green tea infusions from camellia ptilophylla, a peculiar tea variety with 6.0% of theobromine, and camellia sinensis as the control for comparative purpose. numerous negatively charged spherical colloidal particles of 50-100 nm in diameter were primarily found in both green tea infusions. catec ...201728412808
pestalotiopsis and allied genera from camellia, with description of 11 new species from china.a total of 124 pestalotiopsis-like isolates associated with symptomatic and asymptomatic tissues of camellia sinensis and other camellia spp. from eight provinces in china were investigated. based on single- and multi-locus (its, tef, tub2) phylogenies, as well as morphological characters, host associations and geographical distributions, they were classified into at least 19 species in three genera, i.e. neopestalotiopsis, pestalotiopsis and pseudopestalotiopsis. eight novel species in pestalot ...201728408743
bionic multi-tentacled ionic liquid-modified silica gel for adsorption and separation of polyphenols from green tea (camellia sinensis) leaves.for the first time, a new kind of bionic multi-tentacled ionic liquid-modified silica gel was synthesized and used as selective adsorbent in the preparative separation of tea polyphenols (tp) from green tea leaves. it was found that silica particles with polyvinyl alcohol chains modified by n-methyl imidazole proline salt (pva·im+·pro-@sio2) had the excellent performance in selective adsorption as well as fine separation for target compounds. the new adsorbent exhibited high adsorption capacity ...201728407961
a new cineol derivative, polyphenols and norterpenoids from saharan myrtle tea (myrtus nivellei): isolation, structure determination, quantitative determination and antioxidant activity.the phytochemical profile of decoction and infusion, obtained from the dried leaves of m. nivellei, consumed as tea in saharan region, was characterized by uhplc-pda-hrms. fourteen compounds were characterized and, to confirm the proposed structures a preparative procedure followed by nmr spectroscopy was applied. compound 3 (2-hydroxy-1,8-cineole disaccharide) was a never reported whereas a bicyclic monoterpenoid glucoside (2), two ionol glucosides (1 and 12), a tri-galloylquinic acid (4), two ...201728363507
[influence of luxury foodstuffs on glaucoma].the term "luxury foodstuffs" refers to foods which are not consumed because of their nutritional value. classic modern luxury foodstuffs are alcohol, caffeine-containing drinks, cocoa, sugar and tobacco. the following review article examines some of these modern luxury foodstuffs in detail, as well as their influence on glaucoma. thus, small quantities of alcohol lower high intraocular pressure and have a positive influence on the blood circulation of the optic nerve. in addition, red wine polyp ...201728142165
[progress in catechins oxidation products and their formation mechanism].catechins are the key components of tea and have a great impact on its quality. catechins can be oxidized to form a new black tea polyphenols, some of which have better pharmacological effect. however, the formation mechanism of these new polyphenols is still unclear. in this paper, oxidation products coming from catechins and the formation mechanism of the new compounds are reviewed.it is the base of further study on theaflavins, thearubigins and theabrownines.201728948726
theaflavin ameliorates ionizing radiation-induced hematopoietic injury via the nrf2 pathway.it has been well established that reactive oxygen species (ros) play a critical role in ionizing radiation (ir)-induced hematopoietic injury. theaflavin (tf), a polyphenolic compound from black tea, has been implicated in the regulation of endogenous cellular antioxidant systems. however, it remains unclear whether tf could ameliorate ir-induced hematopoietic injury, particularly the hematopoietic stem cell (hsc) injury. in this study, we explored the potential role of tf in ir-induced hsc injur ...201728939421
comparative effects of green and black tea extracts on lowering serum uric acid in hyperuricemic mice.tea (camellia sinensis (l.) kuntze [theaceae]) is used to induce urination and inducing nervous excitation. green and black teas have multifarious physiological functions. the different effects of green and black tea aqueous extracts (gtes and btes) on hyperuricemia are not definitely reported.201728938867
green tea (camellia sinensis) and l-theanine: medicinal values and beneficial applications in humans-a comprehensive review.green tea (camellia sinensis) is a famous herb, and its extract has been extensively used in traditional chinese medicinal system. in this context, several studies have revealed its health benefits and medicinal potentialities for several ailments. with ever increasing scientific knowledge, search for safer, potential and novel type of health-related supplements quest, scientists are re-directing their research interests to explore natural resources i.e. medicinal herbs/plant derived compounds. ...201728938517
molecular aspects of cancer chemopreventive and therapeutic efficacies of tea and tea polyphenols.the natural dietary product tea (camellia sinensis) and its bioactive polyphenols such as epigallocatechin gallate (egcg) and theaflavin (tf) demonstrated potential anticancer effects in different preclinical and clinical studies. the aim of the present review was to understand the molecular mechanisms of the tea and tea polyphenol-mediated cancer prevention and therapy. in the setting of in vivo cancer prevention studies, administration of the tea and tea polyphenols at preinitiation stages onl ...201728935149
effects of long-term ingestion of white tea on oxidation produced by aging and acute oxidative damage in rats.the infusion tea extracted from the leaves of the plant camellia sinensis can be used in the prevention of cancer, cardiovascular and neurodegenerative diseases, and aging, while adriamycin (adr) is an anticancer drug that increases oxidative stress in cells. the present study evaluated the protective effect of the long-term consumption of white tea used at two different doses against the oxidative stress produced by aging and acute oxidation caused adr treatment. at wearing, rats received disti ...201728929453
genome-wide analysis of the biosynthesis and deactivation of gibberellin-dioxygenases gene family in camellia sinensis (l.) o. kuntze.gibberellins (gas), a class of diterpenoid phytohormones, play a key role in regulating diverse processes throughout the life cycle of plants. bioactive ga levels are rapidly regulated by gibberellin-dioxygenases (gaox), which are involved in the biosynthesis and deactivation of gibberellin. in this manuscript, a comprehensive genome-wide analysis was carried out to find all gaox in camellia sinensis. for the first time in a tea plant, 14 csgaox genes, containing two domains, diox_n (pf14226) an ...201728925957
color stability of lithium disilicate ceramics after aging and immersion in common beverages.the color of an esthetic restoration and its color stability are important for long-term success. however, the impact of common beverages on lithium disilicate ceramic is not well known.201728923549
cell wall pectin methyl-esterification and organic acids of root tips involve in aluminum tolerance in camellia sinensis.tea plant (camellia sinensis (o.) kuntze) can survive from high levels of aluminum (al) in strongly acidic soils. however, the mechanism driving its tolerance to al, the predominant factor limiting plant growth in acid condition, is still not fully understood. here, two-year-old rooted cuttings of c. sinensis cultivar 'longjingchangye' were used for al resistance experiments. we found that the tea plants grew better in the presence of 0.4 mm al than those grew under lower concentration of al tre ...201728917145
draft genome sequence of paenibacillus sp. xy044, a potential plant growth promoter isolated from a tea plant.paenibacillus sp. xy044 is an endophytic bacterium isolated from the stem of a tea plant (camellia sinensis cv. maoxie). here, we present the draft genome sequence of xy044, which includes genes encoding features related to plant growth promotion and biocontrol.201728912334
draft genome sequence of streptomyces sp. xy006, an endophyte isolated from tea (camellia sinensis).streptomyces sp. xy006 is an endophytic bacterium isolated from the young leaf material of the tea plant (camellia sinensis). the draft genome consists of 8.2 mb and encodes 7,415 putative open reading frames. this strain is found to contain a high capacity for the production of natural products.201728912322
dna barcode and identification of the varieties and provenances of taiwan's domestic and imported made teas using ribosomal internal transcribed spacer 2 sequences.the major aim of made tea identification is to identify the variety and provenance of the tea plant. the present experiment used 113 tea plants [camellia sinensis (l.) o. kuntze] housed at the tea research and extension substation, from which 113 internal transcribed spacer 2 (its2) fragments, 104 trnl intron, and 98 trnl-trnf intergenic sequence region dna sequences were successfully sequenced. the similarity of the its2 nucleotide sequences between tea plants housed at the tea research and ext ...201728911667
optimisation of saponin extraction conditions with camellia sinensis var. assamica seed and its application for a natural detergent.camellia sinensis var. assamica seed cake (a by-product of tea-seed oil) is an abundant resource with poor utilisation. c. sinensis var. assamica seed saponin (css) is one kind of non-ionic surfactant. in this study, the css extraction conditions were optimised by response surface methodology (rsm) and then the css detergent was developed. additionally, the safety and decontamination ability of the developed detergent were evaluated.201728990656
phosphate stresses affect ionome and metabolome in tea plants.in order to study the response of tea plants to p stress, we conducted the ionomic and metabolomic analysis by icp-oes, gc-ms and lc-ms. the results demonstrated that p was antagonistic with s, and was cooperative with cu, zn, mn and fe under p-deficiency. however, p was antagonistic with mn, fe and s, and was cooperative with cu and zn under p-excess. moreover, p-deficiency or excess reduced the syntheses of flavonoids and phosphorylated metabolites. p-deficiency decreased the amount of glutama ...201728982053
inhibitory effects of total triterpenoid saponins isolated from the seeds of the tea plant (camellia sinensis) on human ovarian cancer cells.ovarian cancer is regarded as one of the most severe malignancies for women in the world. death rates have remained steady over the past five decades, due to the undeniable inefficiency of the current treatment in preventing its recurrence and death. the development of new effective alternative agents for ovarian cancer treatment is becoming increasingly critical. tea saponins (ts) are triterpenoidsaponins composed of sapogenins, glycosides, and organic acids, which possess a variety of pharmaco ...201728974006
α-farnesene and ocimene induce metabolite changes by volatile signaling in neighboring tea (camellia sinensis) plants.herbivore-induced plant volatiles (hipvs) act as direct defenses against herbivores and as indirect defenses by attracting herbivore enemies. however, the involvement of hipvs in within-plant or plant-to-plant signaling is not fully clarified. furthermore, in contrast to model plants, hipv signaling roles in crops have hardly been reported. here, we investigated hipvs emitted from tea (camellia sinensis) plants, an important crop used for beverages, and their involvement in tea plant-to-plant si ...201728969800
modeling of yield and environmental impact categories in tea processing units based on artificial neural networks.in this study, an artificial neural network (ann) model was developed for predicting the yield and life cycle environmental impacts based on energy inputs required in processing of black tea, green tea, and oolong tea in guilan province of iran. a life cycle assessment (lca) approach was used to investigate the environmental impact categories of processed tea based on the cradle to gate approach, i.e., from production of input materials using raw materials to the gate of tea processing units, i. ...201728965294
decaffeinated green and black tea polyphenols decrease weight gain and alter microbiome populations and function in diet-induced obese mice.decaffeinated green tea (gt) and black tea (bt) polyphenols inhibit weight gain in mice fed an obesogenic diet. since the intestinal microflora is an important contributor to obesity, it was the objective of this study to determine whether the intestinal microflora plays a role in the anti-obesogenic effect of gt and bt.201728965248
digestion under saliva, simulated gastric and small intestinal conditions and fermentation in vitro of polysaccharides from the flowers of camellia sinensis induced by human gut microbiota.in the present study, digestion under saliva, simulated gastric and small intestinal conditions and fermentation in vitro of polysaccharides from the flowers of camellia sinensis (tfps) by human gut microbiota were investigated. the results indicated that human saliva and simulated gastric and intestinal juices had no effect on tfps, while tfps could be utilized by human fecal microbiota, which was proved from the decreased molecular weight and lower content of total or reducing sugars after fer ...201729143827
antioxidant and anti-inflammatory properties of ilex guayusa tea preparations: a comparison to camellia sinensis teas.ilex guayusa tea preparations are now commercially available as runa tea. little is known regarding the antioxidant and anti-inflammatory bioactivities of this tea. the i. guayusa teas had a total polyphenolic content between 54.39 and 67.23 mg gae per g dry mass and peroxyl radical scavenging capacities between 1773.41 and 2019 μmol te per g dry mass, nearly half of that for the camellia sinensis teas. the i. guayusa teas afforded 60-80% protection from oxidative stress in the caco-2 cellular a ...201729134218
functional characterization of epitheaflagallin 3-o-gallate generated in laccase-treated green tea extracts in the presence of gallic acid.epitheaflagallin (etfg) and epitheaflagallin 3-o-gallate (etfgg) are minor polyphenols in black tea extract that are enzymatically synthesized from epigallocatechin (egc) and epigallocatechin gallate (egcg), respectively, in green tea extract via laccase oxidation in the presence of gallic acid. the constituents of laccase-treated green tea extract in the presence of gallic acid are thus quite different from those of nonlaccase-treated green tea extract: egc and egcg are present in lower concent ...201729131612
proteomic analysis of camellia sinensis (l.) reveals a synergistic network in the response to drought stress and recovery.drought is a crucial limiting factor for tea yield and quality. to systematically characterize the molecular response of tea plants to drought stress and its capacity to recover, we used itraq-based comparative proteomic approach to investigate the effects of drought on protein expression profiles in tea seedlings subjected to different drought treatments. a total of 3274 proteins were identified, of which 2169 and 2300 showed differential expressions during drought and recovery, respectively. f ...201729096085
functional analysis of two flavanone-3-hydroxylase genes from camellia sinensis: a critical role in flavonoid accumulation.flavonoids are major secondary metabolites in camellia sinensis. flavanone-3-hydroxylase (f3h) is a key enzyme in flavonoid biosynthesis in plants. however, its role in the flavonoid metabolism in c. sinensis has not been well studied. in this study, we cloned two f3hs from c. sinensis, named csf3ha and csf3hb, where csf3ha containing 1107 bases encoded 368 amino acids, and csf3hb containing 1071 bases encoded 357 amino acids. enzymatic activity analysis showed both recombinant csf3h enzymes in ...201729088063
comparative assessment of antibacterial efficacy of aqueous extract of commercially available black, green, and lemon tea: an in vitro study.the objective of this study is to determine and compare antibacterial efficacy of aqueous extracts of black, green, and lemon tea of a commercially available brand.201729085267
improving the quality of fermented black tea juice with oolong tea infusion.fresh tea leaves were crushed into juice and then fermented (oxidation) to obtain fermented black tea juice, which can be used to prepare black tea beverage. the effects of addition of oolong tea infusion or tossing of tea leaves on the sensory quality and theaflavins (tfs) concentration of fermented black tea juice were investigated. the results showed that both addition of tea infusion and tossing of fresh tea leaves increased the tfs/thearubigins (trs) ratio and improved the sensory quality o ...201729085133
quantum-dot-based lateral flow immunoassay for detection of neonicotinoid residues in tea leaves.neonicotinoid insecticides are commonly used for pest control on tea plantations as a result of their broad-spectrum activity. however, neonicotinoid residues released from tea leaves into tea infusions pose a dietary risk to consumers. therefore, a rapid, sensitive, and reliable on-site detection method for neonicotinoids is needed. we developed a quantum-dot-based fluorescent lateral flow immunochromatographic strip (lfics) combined with a broad-specific antibody for detection of typical neoni ...201729077402
actual daily intakes of tea catechins and thier estimation according to four season 3 day weighed dietary records and a short food frequency questionnaire among japanese men and womenbackground: tea catechins are considered to be important preventive factors of cancer on several organs; however, the relationships of the actual daily intakes (adis) on the preventive effects have not been adequately addressed. we measured the adis of tea catechins as annual averages derived from every their ingested cups recorded by each subject, and the estimation models were established considering tea origin. methods: fifty-nine japanese men and women completed four season 3 day weighed die ...201729072829
pupation behaviors and emergence successes of ectropis grisescens (lepidoptera: geometridae) in response to different substrate types and moisture contents.ectropis grisescens warren (lepidoptera: geometridae) is one of the most severe pests of tea plants in china. this species commonly pupates in soil; however, little is known about its pupation ecology. in the present study, choice and no-choice tests were conducted to investigate the pupation behaviors and emergence success of e. grisescens in response to different substrates (sand, sandy loam 1, sandy loam 2, and silt loam) and moisture contents (5, 20, 35, 50, 65, and 80%). moisture-choice bio ...201729069472
multimycotoxin lc-ms/ms analysis in tea beverages after dispersive liquid-liquid microextraction (dllme).the aim of the present study was to develop a multimycotoxin liquid chromatography tandem mass spectrometry (lc-ms/ms) method with a dispersive liquid-liquid microextraction procedure (dllme) for the analysis of afs, 3adon, 15adon, niv, ht-2, t-2, zea, ota, enns, and bea in tea beverages and to evaluate their mycotoxin contents. the proposed method was characterized in terms of linearity, limits of detection (lods), limits of quantification (loqs), recoveries, repeatability (intraday precision), ...201729068686
the roles of epigallocatechin-3-gallate in the treatment of neuropathic pain: an update on preclinical in vivo studies and future perspectives.neuropathic pain (np) is a complex and chronic disease caused by lesions or defects of the somatosensory nervous system. the treatments normally used for managing np usually lack efficacy. several animal models of np have been engineered in order to understand the molecular mechanisms underlying np and to find alternative molecules to use as new therapeutic agents. preclinical in vivo studies identified the epigallocatechin-3-gallate (egcg), a main active component of green tea (camellia sinensi ...201729066865
comprehensive lipidome-wide profiling reveals dynamic changes of tea lipids during manufacturing process of black tea.as important biomolecules in camellia sinensis l., lipids undergo substantial changes during black tea manufacture, which is considered to contribute to tea sensory quality. however, limited by analytical capacity, detailed lipid composition and its dynamic changes during black tea manufacture remain unclear. herein, we performed tea lipidome profiling using high resolution liquid chromatography coupled to mass spectrometry (lc-ms), which allows simultaneous and robust analysis of 192 individual ...201729058896
development of a novel herbal formulation to inhibit biofilm formation in toxigenic vibrio cholerae.vibrio cholerae, a causative agent of the waterborne disease cholera, still threatens a large proportion of world's population. the role of biofilm formation in v. cholerae pathogenesis is well established, as it provides the bacterium enhanced tolerance to antimicrobial agents and increased transmission. in the present study, four medicinal plants used in traditional medicines with antidiarrheal properties were evaluated for its antibiofilm activity. methanol extracts of these plants (centella ...201729053421
characterization of molecular structures of theaflavins and the interactions with bovine serum albumin.in this study, theaflavins (tf1, tf2a, tf2b and tf3) were prepared from black tea and their interaction with bovine serum albumin (bsa) was explored by fluorescence and cd spectroscopy. the results showed that the structures of theaflavins exhibited significant effects on the binding/quenching process, and the binding affinity increased with the increase of molecular weight of theaflavins and the presence of galloyl moiety. the quenching effects showed a sequence as tf3 > tf2a > tf2b > tf1, demo ...201729051637
genome-wide identification of micrornas responsive to ectropis oblique feeding in tea plant (camellia sinensis l.).the tea plant (camellia sinensis l.) is vulnerable to the geometrid ectropis oblique; although micrornas (mirnas) are important for plant growth, development and stress response, the function of mirnas in the response of c. sinensis to stress from e. oblique is unclear. to identify e. oblique stress-responsive mirnas and their target genes in tea plant, three small rna libraries were constructed from leaves subjected to mechanical wounding (mw), geometrid attack (ga) and from healthy control (ck ...201729051614
inhibitory effect of black tea pigments, theaflavin‑3/3'-gallate against cisplatin-resistant ovarian cancer cells by inducing apoptosis and g1 cell cycle arrest.adverse side effects and acquired resistance to conventional chemotherapy based on platinum drive the exploration of other selective anticancer drugs. theaflavin‑3-gallate (tf2a) and theaflavin‑3'-gallate (tf2b), theaflavin monomers in black tea, exhibited a potent growth inhibitory effect on cisplatin-resistant ovarian cancer a2780/cp70 cells and were less cytotoxic to normal ovarian iose-364 cell line. flow cytometry analysis and western blotting indicated that tf2a and tf2b induced apoptosis ...201729048667
a review of the taea proficiency test on natural and anthropogenic radionuclides activities in black tea.a proficiency test amongst 15 turkish laboratories with participation of 5 non-turkish laboratories was organized to determine the 137cs, 40k and 90sr massic activities in black tea powder samples. the bulk material, consisting of tea produced in 2014, was mixed with contaminated tea that was withdrawn from the market after the chernobyl accident. nineteen laboratories reported 41 results. the evaluation of the results was based on the accuracy and precision criteria adopted by the iaea proficie ...201729046243
comparative suppressing effects of black and green teas on the formation of advanced glycation end products (ages) and age-induced oxidative stress.this study aimed at investigating and comparing the anti-diabetic potential of black and green teas. biochemical analyses indicate higher antioxidant potency, significantly correlated with the phytochemicals present, in green teas compared to black teas. both extracts afforded a similar level of protection to erythrocytes against peroxyl radical-induced lysis. non-cytotoxic concentration of green and black tea extracts significantly reduced the reactive oxygen species (ros) production (p < 0.01) ...201729043349
cellular injury to 1- to 3+-year-old stems of camellia sinensis by tuckerella japonica.tuckerella japonica ehara (acari: tuckerellidae) feeds on predigested plant cells beneath exposed periderm tissue of 1- to 3+-year-old stems of camellia sinensis (l.) o. kuntze (theaceae) where longitudinal bark splitting occurs. control samples from these tissues were compared with areas fed upon by t. japonica adults and immatures to characterize types of cellular injury. stylet diameters ranged from 1.6 to 2.3 µm and were consistent with observed stylet punctures in the stems. mite saliva was ...201729188400
influence of particle size and extraction solvent on antioxidant properties of extracts of tea, ginger, and tea-ginger blend.the influence of particle size and extraction solvent on the antioxidant properties of aqueous and ethanolic extracts of tea (camellia sinensis), ginger (zingiber officinale), and tea-ginger (2:1) blend was investigated. the powder sizes studied were 0.425, 0.710, and 1.180 mm. extracts were analyzed for dpph radical scavenging activity, abts radical scavenging activity, total phenol content (tpc), iron chelating activity, total flavonoid content, and peroxide scavenging activity. the powder wit ...201729188046
nutritional supplements and herbal medicines for women with polycystic ovary syndrome; a systematic review and meta-analysis.polycystic ovary syndrome (pcos) is a common, reproductive endocrinopathy associated with serious short and long term health risks. many women with pcos use ingestible complementary medicines. this systematic review examined the effect on menstrual regulation and adverse effects from randomised controlled trials.201729178904
colletotrichum gloeosporioides- contaminated tea infusion blocks lipids reduction and induces kidney damage in mice.when the homogenate of fresh tea tree leaves was fermented to produce black tea beverage, the colletotrichum gloeosporioides (main pathogen or endophyte of camellia sinensis) may be mixed into the fermentation liquor. however, it was unclear whether c. gloeosporioides-contaminated tea beverage would damage human health. therefore, we investigated the changes of functional components and the influences on mice. c. gloeosporioides was added to the green tea infusion. after cultivation of 48 h, tea ...201729163391
functional analysis of an uridine diphosphate glycosyltransferase involved in the biosynthesis of polyphenolic glucoside in tea plants (camellia sinensis).polyphenols are one of the largest groups of compounds that confer benefits to the health of plants and humans. flavonol glycosides are a major ingredient of polyphenols in camellia sinensis. flavonol-3-o-glycosides are characteristic astringent taste compounds in tea infusion. a polyphenolic glycosyltransferase (csugt72am1) belonging to cluster iiib was isolated from the tea plant. the full-length cdna of csugt72am1 is 1416 bp. it encodes 472 amino acids with a calculated molecular mass of 50.9 ...201729161813
functional characterization of salicylic acid carboxyl methyltransferase from camellia sinensis, providing the aroma compound of methyl salicylate during the withering process of white tea.methyl salicylate (mesa) is one of the volatile organic compounds (vocs) that releases floral scent and plays an important role in the sweet flowery aroma of tea. during the withering process for white tea producing, mesa was generated by salicylic acid carboxyl methyltransferase (samt) with salicylic acid (sa), and the specific floral scent was formed. in this study, we first cloned a cssamt from tea leaves (genbank accession no. mg459470) and used escherichia coli and saccharomyces cerevisiae ...201729160698
identification of drought-responsive mirnas and physiological characterization of tea plant (camellia sinensis l.) under drought stress.drought stress is one of the major natural challenges in the main tea-producing regions of china. the tea plant (camellia sinensis) is a traditional beverage plant whose growth status directly affects tea quality. recent studies have revealed that micrornas (mirnas) play key functions in plant growth and development. although some mirnas have been identified in c. sinensis, little is known about their roles in the drought stress response of tea plants.201729157225
comparative proteomics reveals the physiological differences between winter tender shoots and spring tender shoots of a novel tea (camellia sinensis l.) cultivar evergrowing in winter.a recently discovered tea [camellia sinensis (l.) o. kuntze] cultivar can generate tender shoots in winter. we performed comparative proteomics to analyze the differentially accumulated proteins between winter and spring tender shoots of this clonal cultivar to reveal the physiological basis of its evergrowing character during winter.201729157222
genome-wide identification of conserved and novel micrornas in one bud and two tender leaves of tea plant (camellia sinensis) by small rna sequencing, microarray-based hybridization and genome survey scaffold sequences.micrornas (mirnas) are important for plant growth and responses to environmental stresses via post-transcriptional regulation of gene expression. tea, which is primarily produced from one bud and two tender leaves of the tea plant (camellia sinensis), is one of the most popular non-alcoholic beverages worldwide owing to its abundance of secondary metabolites. a large number of mirnas have been identified in various plants, including non-model species. however, due to the lack of reference genome ...201729157210
phenolic compounds as natural and multifunctional anti-obesity agents: a review.prevalence of obesity worldwide has reached pandemic proportions. despite the increasing evidence in the implication of phenolic compounds in obesity management, the real effect is not completely understood. the available in vitro and in vivo studies have demonstrated the implication of phenolic compounds in: lowering food intake, decreasing lipogenesis, increasing lipolysis, stimulating fatty acids β-oxidation, inhibiting adipocyte differentiation and growth, attenuating inflammatory responses ...201729156939
functional foods and lifestyle approaches for diabetes prevention and management.functional foods contain biologically active ingredients associated with physiological health benefits for preventing and managing chronic diseases, such as type 2 diabetes mellitus (t2dm). a regular consumption of functional foods may be associated with enhanced anti-oxidant, anti-inflammatory, insulin sensitivity, and anti-cholesterol functions, which are considered integral to prevent and manage t2dm. components of the mediterranean diet (md)-such as fruits, vegetables, oily fish, olive oil, ...201729194424
proteome and acetyl-proteome profiling of camellia sinensis cv. 'anjin baicha' during periodic albinism reveals alterations in photosynthetic and secondary metabolite biosynthetic pathways.tea leaf color is not only important from an aesthetics standpoint but is also related to tea quality. to investigate the molecular mechanisms that determine tea leaf color, we examined camellia sinensis cv. 'anjin baicha' (an albino tea cultivar) by tandem mass tag isobaric labeling to generate a high-resolution proteome and acetyl-proteome atlas of three leaf developmental stages. we identified a total of 7,637 proteins and quantified 6,256; of these, 3,232 were classified as differentially ac ...201729312376
fat burner-induced acute liver injury: case series of four patients.dietary supplements known as "fat burners" are typically marketed with claims of increasing energy expenditure through alterations in fat metabolism. they are marketed as natural products and their use is thus perceived as a safe body weight reduction strategy. we report on five episodes of liver injury in four patients. liver injury was associated with consumption of different commercially available fat burners: green tea extract (camellia sinensis), garcinia gummi-gutta, green coffee beans, an ...201729310849
comparison of the effects of three tea cultivars (camellia sinensis l.) on nitric oxide production and aortic soluble guanylate cyclase expression in high-salt diet-fed spontaneously hypertensive rats.studies have suggested that the consumption of green tea reduces the risk of cardiovascular diseases. although epigallocatechin gallate (egcg) is the best studied active substance characteristic of green tea, previous results on egcg do not appear sufficient to explain completely the mechanism of cardiovascular protection by green tea. therefore, we investigated the effect of three different tea cultivars, "yabukita," "sofu," and "sunrouge," which have characteristic flavonoid compositions, on t ...201729225315
comparative analysis of the response and gene regulation in cold resistant and susceptible tea plants.cold environment is the main constraint for tea plants (camellia sinensis) distribution and tea farming. we identified two tea cultivars, called var. sinensis cv. shuchazao (scz) with a high cold-tolerance and var. assamica cv. yinghong9 (yh9) with low cold-tolerance. to better understand the response mechanism of tea plants under cold stress for improving breeding, we compared physiological and biochemical responses, and associated genes expression in response to 7-day and 14-day cold acclimati ...201729211766
tracking residual organochlorine pesticides (ocps) in green, herbal, and black tea leaves and infusions of commercially available tea products marketed in poland.the content of residual organochlorine pesticides (ocps) was examined in green, herbal, and black tea leaves as well as in their infusions prepared from tea products marketed in the main supermarkets in poland. it was found that the detected mean levels of organochlorine residues in tea leaves ranged from <lod to 16.36 ng g-1 dry weight. among hexachlorocyclohexane isomers, γ-hch in green tea occurred in the highest concentrations. among dichlorodiphenyltrichloroethane (ddt) metabolites the high ...201729210611
an in vitro study to compare the effect of different types of tea with chlorhexidine on streptococcusmutans.tea is the second most commonly consumed beverage in the world after water. the leaf and bud of the plant camellia sinensis produces tea. the different forms of tea are 'non-fermented' green tea, 'semi-fermented' oolong tea and 'fermented' black tea according to the manufacturing process. streptococcus mutans is the main causative organism in dental caries and plaque formation.201729207823
development of a rapid and simple method to remove polyphenols from plant extracts.polyphenols are secondary metabolites of plants, which are responsible for prevention of many diseases. polyvinylpolypyrrolidone (pvpp) has a high affinity towards polyphenols. this method involves the use of pvpp column to remove polyphenols under centrifugal force. standards of gallic acid, epigallocatechin gallate, vanillin, and tea extracts (camellia sinensis) were used in this study. pvpp powder was packed in a syringe with different quantities. the test samples were layered over the pvpp c ...201729201056
pre-treated theaflavin-3,3'-digallate has a higher inhibitory effect on the hct116 cell line.the pro-apoptotic and inhibitory effects of the aflavin-3,3'-digallate (tfdg), which is the typical pigment in black tea, have been demonstrated in many cancer cell lines. however, tfdg is not stable in general culture conditions. so, to what extent tfdg or which degradation products of tfdg play an antitumor role is still unclear. in this study, we evaluated the effect of different treatments of tfdg on hct116 cells. compared with the control, both tfdg and o-tfdg (the tfdg that was pre-incubat ...201729200992
contamination of tea and tea infusion with polycyclic aromatic hydrocarbons.the aim of this work was to validate the method of determination of polycyclic aromatic hydrocarbons (pahs), i.e., benzo(a)pyrene and sum of benzo(a)pyrene, benz(a)anthracene, benzo(b)fluoranthene and chrysene in different types of tea, as well as to assess the transfer of these contaminants from tea to tea infusion. the research materials were popular types of black, green, red and white tea. quantitative and qualitative determination of pahs was performed by high performance liquid chromatogra ...201729283369
comparative effect of camellia sinensis teas on object recognition test deficit and metabolic changes induced by cafeteria diet.consumption of high-fat and high-sugar diets in western countries has increased significantly causing major global health problems including metabolic syndrome and obesity. in addition, studies have suggested that obesity can lead to learning and memory deficits. in this context, the use of natural compounds with low costs, minor side effects and increased antioxidant activity, such as teas, could reduce the damages induced by obesity. we investigated the effect of white, green, red, and black t ...201729280418
shade changing effectiveness of plasdone and blue covarine-based whitening toothpaste on teeth stained with chlorhexidine and black tea.this study evaluated the effectiveness of toothbrushing with whitening toothpaste in altering the shade of stained human enamel.201729279667
green synthesis palladium nanoparticles mediated by white tea (camellia sinensis) extract with antioxidant, antibacterial, and antiproliferative activities toward the human leukemia (molt-4) cell line.among nanoparticles used for medical applications, palladium nanoparticles (pdnps) are among the least investigated. this study was undertaken to develop pdnps by green synthesis using white tea (w.tea; camellia sinensis) extract to produce the pd@w.tea nps. the pd@w.tea nps were characterized by uv-vis spectroscopy and x-ray diffractometry, and evaluated with transmission electron microscopy (tem) and scanning electron microscopy (sem). the pd@w.tea nps were spherical (size 6-18 nm) and contain ...201729276385
external morphology of the mouthparts and observations on behavior of tuckerella japonica on camellia sinensis in the continental usa.tuckerella japonica ehara (acari: tetranychoidea: tuckerellidae) was found on stems of seedling plants of camellia sinensis (l.) o. kuntze (theaceae) in the continental usa. this mite is able to pierce exposed green periderm tissue with its paired stylets on 1- to 3- or 4-year-old stems where the outer bark had split longitudinally. the mite was not found on branches older than 3 or 4 years, where splitting diminished and, eventually, a uniform covering of bark was formed. the mouthparts of t. j ...201729255965
effect of various teas on color stability of resin composites.to investigate the effect of various teas on color stability of resin composites.201729251455
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