Publications

gscml27, a gene encoding a calcium-binding ef-hand protein from glycine soja, plays differential roles in plant responses to bicarbonate, salt and osmotic stresses.	calcium, as the most widely accepted messenger, plays an important role in plant stress responses through calcium-dependent signaling pathways. the calmodulin-like family genes (cmls) encode ca2+ sensors and function in signaling transduction in response to environmental stimuli. however, until now, the function of plant cml proteins, especially soybean cmls, is largely unknown. here, we isolated a glycine soja cml protein gscml27, with four conserved ef-hands domains, and identified it as a cal ...	2015	26550992
[distribution characteristics of soil nematodes in reclaimed land of copper-mine-tailings in different plant associations].	a survey was carried out to investigate soil nematode communities in the plant associations of gramineae (arthraxon lanceolatus, al; imperata cylindrica, ic) and leguminosae (glycine soja, gs) in reclaimed land of copper-mine-tailings and in the plant associations of gramineae (digitaria chrysoblephara, dc-ck) of peripheral control in fenghuang mountain, tongling city. a total of 1277 nematodes were extracted and sorted into 51 genera. the average individual density of the nematodes was 590 indi ...	2015	26094476
ectopic expression of a glycine soja myo-inositol oxygenase gene (gsmiox1a) in arabidopsis enhances tolerance to alkaline stress.	myo-inositol participates in various aspects of plant physiology, and myo-inositol oxygenase is the key enzyme of the myo-inositol oxygenation pathway. previous studies indicated that myo-inositol oxygenase may play a role in plant responses to abiotic stresses. in this study, we focused on the functional characterization of gsmiox1a, a remarkable alkaline stress-responsive gene of glycine soja 07256, based on rna-seq data. using quantitative real-time pcr, we demonstrated that gsmiox1a is rapid ...	2015	26091094
overexpression of gsgstu13 and scmrp in medicago sativa confers increased salt-alkaline tolerance and methionine content.	tau-class glutathione s-transferases (gstus) are ubiquitous proteins encoded by a large gene family in plants, which play important roles in combating different environmental stresses. in previous studies, we constructed a glycine soja transcriptional profile, and identified three gstus (gsgstu13, gsgstu14 and gsgstu19) as potential salt-alkaline stress-responsive genes. two of them, gsgstu14 and gsgstu19, have been shown to positively regulate plant salt-alkaline tolerance. in this study, we fu ...	2015	26010993
wild soybean roots depend on specific transcription factors and oxidation reduction related genesin response to alkaline stress.	soil alkalinity is an important environmental problem limiting agricultural productivity. wild soybean (glycine soja) shows strong alkaline stress tolerance, so it is an ideal plant candidate for studying the molecular mechanisms of alkaline tolerance and identifying alkaline stress-responsive genes. however, limited information is available about g. soja responses to alkaline stress on a genomic scale. therefore, in the present study, we used rna sequencing to compare transcript profiles of g. ...	2015	25874911
gsskp21, a glycine soja s-phase kinase-associated protein, mediates the regulation of plant alkaline tolerance and aba sensitivity.	plant skp1-like family proteins, components of the scf complex e3 ligases, are involved in the regulation of plant development and stress responses. little is known about the precise function of skp genes in plant responses to environmental stresses. gsskp21 was initially identified as a potential stress-responsive gene based on the transcriptome sequencing of glycine soja. in this study, we found that gsskp21 protein contains highly conserved skp domains in its n terminus and an extra unidentif ...	2015	25477077
comparison of saponin composition and content in wild soybean (glycine soja sieb. and zucc.) before and after germination.	eight wild soybean accessions with different saponin phenotypes were used to examine saponin composition and relative saponin quantity in various tissues of mature seeds and two-week-old seedlings by lc-pda/ms/ms. saponin composition and content were varied according to tissues and accessions. the average total saponin concentration in 1 g mature dry seeds of wild soybean was 16.08 ± 3.13 μmol. in two-week-old seedlings, produced from 1 g mature seeds, it was 27.94 ± 6.52 μmol. group a saponins ...	2014	25127168
identification of quantitative trait loci controlling linolenic acid concentration in pi483463 (glycine soja).	the qtls controlling alpha-linolenic acid concentration from wild soybean were mapped on nine soybean chromosomes with various phenotypic variations. new qtls for alpha-linolenic acid were detected in wild soybean. alpha-linolenic acid (ala) is a polyunsaturated fatty acid desired in human and animal diets. some wild soybean (glycine soja) genotypes are high in ala. the objective of this study was to identify quantitative trait loci (qtls) controlling ala concentration in a wild soybean accessio ...	2014	24794978
allelic variation of soybean flower color gene w4 encoding dihydroflavonol 4-reductase 2.	flower color of soybean is primarily controlled by six genes, viz., w1, w2, w3, w4, wm and wp. this study was conducted to investigate the genetic and chemical basis of newly-identified flower color variants including two soybean mutant lines, 222-a-3 (near white flower) and e30-d-1 (light purple flower), a near-isogenic line (clark-w4), flower color variants (t321 and t369) descended from the w4-mutable line and kw4 (near white flower, glycine soja).	2014	24602314
ectopic expression of gsppck3 and scmrp in medicago sativa enhances plant alkaline stress tolerance and methionine content.	so far, it has been suggested that phosphoenolpyruvate carboxylases (pepcs) and pepc kinases (ppcks) fulfill several important non-photosynthetic functions. however, the biological functions of soybean ppcks, especially in alkali stress response, are not yet well known. in previous studies, we constructed a glycine soja transcriptional profile, and identified three ppck genes (gsppck1, gsppck2 and gsppck3) as potential alkali stress responsive genes. in this study, we confirmed the induced expre ...	2014	24586886
soybean knowledge base (soykb): a web resource for integration of soybean translational genomics and molecular breeding.	soybean knowledge base (http://soykb.org) is a comprehensive web resource developed for bridging soybean translational genomics and molecular breeding research. it provides information for six entities including genes/proteins, micrornas/srnas, metabolites, single nucleotide polymorphisms, plant introduction lines and traits. it also incorporates many multi-omics datasets including transcriptomics, proteomics, metabolomics and molecular breeding data, such as quantitative trait loci, traits and ...	2014	24136998
salinity tolerance in soybean is modulated by natural variation in gmsalt3.	the identification of genes that improve the salt tolerance of crops is essential for the effective utilization of saline soils for agriculture. here, we use fine mapping in a soybean (glycine max (l.) merr.) population derived from the commercial cultivars tiefeng 8 and 85-140 to identify gmsalt3 (salt tolerance-associated gene on chromosome 3), a dominant gene associated with limiting the accumulation of sodium ions (na+) in shoots and a substantial enhancement in salt tolerance in soybean. gm ...	2014	25292417
de novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits.	wild relatives of crops are an important source of genetic diversity for agriculture, but their gene repertoire remains largely unexplored. we report the establishment and analysis of a pan-genome of glycine soja, the wild relative of cultivated soybean glycine max, by sequencing and de novo assembly of seven phylogenetically and geographically representative accessions. intergenomic comparisons identified lineage-specific genes and genes with copy number variation or large-effect mutations, som ...	2014	25218520
a comprehensive analysis of the cupin gene family in soybean (glycine max).	cupin superfamily of proteins, including germin and germin-like proteins (glps) from higher plants, is known to play crucial roles in plant development and defense. to date, no systematic analysis has been conducted in soybean (glycine max) incorporating genome organization, gene structure, expression compendium. in this study, 69 putative cupin genes were identified from the whole-genome of soybean, which were non-randomly distributed on 17 of the 20 chromosomes. these gmcupin proteins were phy ...	2014	25360675
comparative ecophysiological study of salt stress for wild and cultivated soybean species from the yellow river delta, china.	osmotic and ionic stresses were the primary and instant damage produced by salt stress. they can also bring about other secondary stresses. soybean is an important economic crop and the wild soybean aroused increasing attention for its excellent performance in salt resistance. for this reason, we compared the different performances of glycine max l. (zh13) and glycine soja l. (bb52) in both young and mature seedlings, hoping to clarify the specific reasons. our research revealed that, compared t ...	2014	24999494
association mapping of yield-related traits and ssr markers in wild soybean (glycine soja sieb. and zucc.).	wild soybean, the progenitor of cultivated soybean, is an important gene pool for ongoing soybean breeding efforts. to identify yield-enhancing quantitative trait locus (qtl) or gene from wild soybean, 113 wild soybeans accessions were phenotyped for five yield-related traits and genotyped with 85 simple sequence repeat (ssr) markers to conduct association mapping. a total of 892 alleles were detected for the 85 ssr markers, with an average 10.49 alleles; the corresponding pic values ranged from ...	2014	24757383
exploration of presence/absence variation and corresponding polymorphic markers in soybean genome.	this study was designed to reveal the genome-wide distribution of presence/absence variation (pav) and to establish a database of polymorphic pav markers in soybean. the 33 soybean whole-genome sequences were compared to each other with that of williams 82 as a reference genome. a total of 33,127 pavs were detected and 28,912 pav markers with their primer sequences were designed as the database njausoypav_1.0. the pavs scattered on whole genome while only 518 (1.8%) overlapped with simple sequen ...	2014	24751174
major soybean maturity gene haplotypes revealed by snpviz analysis of 72 sequenced soybean genomes.	in this genomics era, vast amounts of next-generation sequencing data have become publicly available for multiple genomes across hundreds of species. analyses of these large-scale datasets can become cumbersome, especially when comparing nucleotide polymorphisms across many samples within a dataset and among different datasets or organisms. to facilitate the exploration of allelic variation and diversity, we have developed and deployed an in-house computer software to categorize and visualize th ...	2014	24727730
identification of active vq motif-containing genes and the expression patterns under low nitrogen treatment in soybean.	plant vq motif-containing protein family plays crucial roles in plant growth, seed development, and defense responses in arabidopsis. however, its function in soybean is still not well defined. we aim to identify the vq gene family, and explore the genetic variation of active gmvq genes in soybean and their expression patterns under low nitrogen stresses. a total of 74 vq motif-containing genes were identified in soybean genome, and were clustered into five distinct subfamilies (gmvqi-v) with ea ...	2014	24727126
role of physiological mechanisms and epsps gene expression in glyphosate resistance in wild soybeans (glycine soja).	the physiological mechanisms underlying glyphosate resistance in wild soybean germplasm and relevant epsps gene expression were evaluated. these germplasms were selected by gradually increasing glyphosate selection pressure started from 2010. as indicated by a whole-plant dose response bioassay, zyd-254 plants were resistant to glyphosate at concentrations of 1230gaeha(-1), but the susceptible plants (zyd-16) were unable to survive in the presence of 300gaeha(-1) glyphosate. the ed50 values of r ...	2014	24581379
population structure and domestication revealed by high-depth resequencing of korean cultivated and wild soybean genomes.	despite the importance of soybean as a major crop, genome-wide variation and evolution of cultivated soybeans are largely unknown. here, we catalogued genome variation in an annual soybean population by high-depth resequencing of 10 cultivated and 6 wild accessions and obtained 3.87 million high-quality single-nucleotide polymorphisms (snps) after excluding the sites with missing data in any accession. nuclear genome phylogeny supported a single origin for the cultivated soybeans. we identified ...	2014	24271940
a thaumatin-like protein, rj4, controls nodule symbiotic specificity in soybean.	soybeans exhibit a nitrogen-fixing symbiosis with soil bacteria of the genera bradyrhizobium and ensifer/sinorhizobium in a unique organ, the root nodule. it is well known that nodulation of soybean is controlled by several host genes referred to as rj (rj) genes. among these genes, a dominant allele, rj4, restricts nodulation with specific bacterial strains such as b. elkanii usda61 and b. japonicum is-34. these incompatible strains fail to invade the host epidermal cells as revealed by observa ...	2014	25059584
interactions between preparations containing female sex hormones and dietary supplements.	an increasing number of premenopausal women use contraception whereas postmenopausal women use hormone replacement therapy (hrt). this long-term hormone therapy poses a high risk of interactions with dietary supplements. taking estrogens at the same time as selective estrogen receptor modulators (serms), biologically-active compounds of glycine soja, ginkgo biloba or pimpinella anisum, may distort the final effect of the hormone agent. on the other hand, estrogen therapy coupled with melatonin o ...	2014	25166453
dt2 is a gain-of-function mads-domain factor gene that specifies semideterminacy in soybean.	similar to arabidopsis thaliana, the wild soybeans (glycine soja) and many cultivars exhibit indeterminate stem growth specified by the shoot identity gene dt1, the functional counterpart of arabidopsis terminal flower1 (tfl1). mutations in tfl1 and dt1 both result in the shoot apical meristem (sam) switching from vegetative to reproductive state to initiate terminal flowering and thus produce determinate stems. a second soybean gene (dt2) regulating stem growth was identified, which, in the pre ...	2014	25005919
a glycine soja 14-3-3 protein gsgf14o participates in stomatal and root hair development and drought tolerance in arabidopsis thaliana.	it is well established that 14-3-3 proteins are key regulators of multiple stress signal transduction cascades. however, the biological functions of soybean 14-3-3 proteins, especially in plant drought response, are not yet known. in this study, we characterized a glycine soja 14-3-3 gene, gsgf14o, which is involved in plant development and drought response. gsgf14o expression was greatly induced by drought stress, as evidenced by the quantitative real-time pcr and β-glucuronidase (gus) activity ...	2014	24272249
gslrpk, a novel cold-activated leucine-rich repeat receptor-like protein kinase from glycine soja, is a positive regulator to cold stress tolerance.	plant lrr-rlks serve as protein interaction platforms, and as regulatory modules of protein activation. here, we report the isolation of a novel plant-specific lrr-rlk from glycine soja (termed gslrpk) by differential screening. gslrpk expression was cold-inducible and shows ser/thr protein kinase activity. subcellular localization studies using gfp fusion protein indicated that gslrpk is localized in the plasma membrane. real-time pcr analysis indicated that temperature, salt, drought, and aba ...	2014	24388511
a novel glycine soja cysteine proteinase inhibitor gscpi14, interacting with the calcium/calmodulin-binding receptor-like kinase gscbrlk, regulated plant tolerance to alkali stress.	it has been well demonstrated that cystatins regulated plant stress tolerance through inhibiting the cysteine proteinase activity under environmental stress. however, there was limited information about the role of cystatins in plant alkali stress response, especially in wild soybean. here, in this study, we focused on the biological characterization of a novel glycine soja cystatin protein gscpi14, which interacted with the calcium/calmodulin-binding receptor-like kinase gscbrlk and positively ...	2014	24407891
[influence of bean yellow mosaic virus on metabolism of photosynthetic pigments, proteins and carbohydrates in glycine soja l].	this paper presents data on bymv effects on some physiological processes of glycine soja l. cultivated in the right-bank forest-steppe regions. pigment content (chlorophyll a, b and carotenoids), soluble proteins and water soluble carbohydrates were estimated and, as has been shown, are subjected to significant changes as compared with control plants, namely: a decrease in the content of chlorophyll a, b and carotenoids was 64%, 53% and 36% compared with the control plants. the significant incre ...	2014	24800515
molecular and biological characterization of an isolate of cucumber mosaic virus from glycine soja by generating its infectious full-genome cdna clones.	molecular and biological characteristics of an isolate of cucumber mosaic virus (cmv) from glycine soja (wild soybean), named as cmv-209, was examined in this study. comparison of nucleotide sequences and phylogenetic analyses of cmv-209 with the other cmv strains revealed that cmv-209 belonged to cmv subgroup i. however, cmv-209 showed some genetic distance from the cmv strains assigned to subgroup ia or subgroup ib. infectious full-genome cdna clones of cmv-209 were generated under the control ...	2014	25288998
pseudorecombination between two distinct strains of cucumber mosaic virus results in enhancement of symptom severity.	recently, a cucumber mosaic virus (cmv) strain, named as cmv-209, was isolated from glycine soja. in this study, symptom expression of cmv-209 was analyzed in detail in nicotiana benthamiana by comparing with that of cmv-fny, which is a representative strain of cmv. using infectious cdna clones of cmv strains 209 and fny, symptom expression of various pseudorecombinants between these two strains were examined in the early and late infection stages. in the early infection stage, the pseudorecombi ...	2014	25289019
[endophytic bacterial diversity of wild soybean (glycine soja) varieties with different resistance to soybean cyst nematode (heterodera glycines)].	the aim of this study was to investigate endophytic bacterial diversity of wild soybean varieties with different resistance to soybean cyst nematode(heterodera glycines) , for deciphering the interactions of soybean cyst nematode with endophytic bacteria.	2014	25345025
overexpression of gmcam4 in soybean enhances resistance to pathogens and tolerance to salt stress.	plant diseases inflict heavy losses on soybean yield, necessitating an understanding of the molecular mechanisms underlying biotic/abiotic stress responses. ca(2) (+) is an important universal messenger, and protein sensors, prominently calmodulins (cams), recognize cellular changes in ca(2) (+) in response to diverse signals. because the development of stable transgenic soybeans is laborious and time consuming, we used the bean pod mottle virus (bpmv)-based vector for rapid and efficient protei ...	2014	24118726
bradyrhizobium ottawaense sp. nov., a symbiotic nitrogen fixing bacterium from root nodules of soybeans in canada.	sixteen strains of symbiotic bacteria from root nodules of glycine max grown in ottawa, canada, were previously characterized and placed in a novel group within the genus bradyrhizobium. to verify their taxonomic status, these strains were further characterized using a polyphasic approach. all strains possessed identical 16s rrna gene sequences that were 99.79 % similar to the closest relative, bradyrhizobium liaoningense lmg 18230(t). phylogenetic analysis of concatenated atpd, glnii, reca, gyr ...	2014	24969302
identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing.	using a whole-genome-sequencing approach to explore germplasm resources can serve as an important strategy for crop improvement, especially in investigating wild accessions that may contain useful genetic resources that have been lost during the domestication process. here we sequence and assemble a draft genome of wild soybean and construct a recombinant inbred population for genotyping-by-sequencing and phenotypic analyses to identify multiple qtls relevant to traits of interest in agriculture ...	2014	25004933
distribution and diversity of rhizobia associated with wild soybean (glycine soja sieb. & zucc.) in northwest china.	a total of 155 nodule isolates that originated from seven sites in northwest china were characterized by pcr-rflp of the 16s rrna gene and sequence analysis of multiple core genes (16s rrna, reca, atpd, and glnii) in order to investigate the diversity and biogeography of glycine soja-nodulating rhizobia. among the isolates, 80 were ensifer fredii, 19 were ensifer morelense, 49 were rhizobium radiobacter, and 7 were putative novel rhizobium species. the phylogenies of e. fredii and e. morelense i ...	2014	25052953
genome re-sequencing of semi-wild soybean reveals a complex soja population structure and deep introgression.	semi-wild soybean is a unique type of soybean that retains both wild and domesticated characteristics, which provides an important intermediate type for understanding the evolution of the subgenus soja population in the glycine genus. in this study, a semi-wild soybean line (maliaodou) and a wild line (lanxi 1) collected from the lower yangtze regions were deeply sequenced while nine other semi-wild lines were sequenced to a 3-fold genome coverage. sequence analysis revealed that (1) no independ ...	2014	25265539
allelic combinations of soybean maturity loci e1, e2, e3 and e4 result in diversity of maturity and adaptation to different latitudes.	soybean cultivars are extremely diverse in time to flowering and maturation as a result of various photoperiod sensitivities. the underlying molecular genetic mechanism is not fully clear, however, four maturity loci e1, e2, e3 and e4 have been molecularly identified. in this report, cultivars were selected with various photoperiod sensitivities from different ecological zones, which covered almost all maturity groups (mg) from mg 000 to mg viii and mg x adapted from latitude n 18° to n 53°. the ...	2014	25162675
genetic and chemical analysis of a key biosynthetic step for soyasapogenol a, an aglycone of group a saponins that influence soymilk flavor.	although certain saponins in soybean seeds have been reported to have health benefits, group a acetyl saponins cause undesirable bitter and astringent tastes in soy products. therefore, reduction or elimination of group a saponins is an important target for soybean breeders. a wide survey of cultivated and wild soybean germplasm identified a mutant line that lacked group a saponins. the absence of soyasapogenol a, a group a saponin aglycone, is controlled by a single recessive allele, sg-5 that ...	2013	23229125
[identification of calcium/calmodulin-binding receptor-like kinase gscbrlk-interactive proteins using yeast two-hybrid system].	gscbrlk (calcium/calmodulin-binding receptor-like kinase from glycine soja) links aba (abscisic acid)- and salt-induced calcium/calmodulin signal in plant cells. in order to study the molecular mechanisms of gscblrk, the salt-treated glycine soja cdna library was screened with pbt3-ste-cbrlk as bait plasmid using yeast two hybrid system. two positive clones (snare and 14-3-3 protein) were identified by constructing cdna library of wild soybean under salt treatment, membrane system yeast two hybr ...	2013	23575546
development and evaluation of soysnp50k, a high-density genotyping array for soybean.	the objective of this research was to identify single nucleotide polymorphisms (snps) and to develop an illumina infinium beadchip that contained over 50,000 snps from soybean (glycine max l. merr.). a total of 498,921,777 reads 35-45 bp in length were obtained from dna sequence analysis of reduced representation libraries from several soybean accessions which included six cultivated and two wild soybean (g. soja sieb. et zucc.) genotypes. these reads were mapped to the soybean whole genome sequ ...	2013	23372807
diversifying selection on flavanone 3-hydroxylase and isoflavone synthase genes in cultivated soybean and its wild progenitors.	soybean isoflavone synthase (ifs) and flavanone 3-hydroxylase (f3h) are two key enzymes catalyzing the biosynthesis of isoflavonoids and flavonoids, both of which play diverse roles in stress responses. however, little is known about the evolutionary pattern of these genes in cultivated soybean and its wild progenitors. herein, we investigated the nucleotide polymorphisms in isoflavone synthase (ifs1, ifs2) and flavanone 3-hydroxylase (f3h2) genes from 33 soybean accessions, including 17 cultiva ...	2013	23342093
identification of wild soybean (glycine soja) tify family genes and their expression profiling analysis under bicarbonate stress.	wild soybean (glycine soja l. g07256) exhibits a greater adaptability to soil bicarbonate stress than cultivated soybean, and recent discoveries show that tify family genes are involved in the response to several abiotic stresses. a genomic and transcriptomic analysis of all tify genes in g. soja, compared with g. max, will provide insight into the function of this gene family in plant bicarbonate stress response. this article identified and characterized 34 tify genes in g. soja. sequence analy ...	2013	23090726
genomic differences between cultivated soybean, g. max and its wild relative g. soja.	glycine max is an economically important crop and many different varieties of soybean exist around the world. the first draft sequences and gene models of g. max (domesticated soybean) as well as g. soja (wild soybean), both became available in 2010. this opened the door for comprehensive comparative genomics studies between the two varieties.	2013	23368680
temperature interactions with transpiration response to vapor pressure deficit among cultivated and wild soybean genotypes.	a key strategy in soybean drought research is increased stomatal sensitivity to high vapor pressure deficit (vpd), which contributes to the 'slow wilting' trait observed in the field. these experiments examined whether temperature of the growth environment affected the ability of plants to respond to vpd, and thus control transpiration rate (tr). two soybean [glycine max (l.) merr.] and four wild soybean [glycine soja (sieb. and zucc.)] genotypes were studied. the tr was measured over a range of ...	2013	22989317
molecular footprints of domestication and improvement in soybean revealed by whole genome re-sequencing.	artificial selection played an important role in the origin of modern glycine max cultivars from the wild soybean glycine soja. to elucidate the consequences of artificial selection accompanying the domestication and modern improvement of soybean, 25 new and 30 published whole-genome re-sequencing accessions, which represent wild, domesticated landrace, and chinese elite soybean populations were analyzed.	2013	23984715
comparative metabolomics in glycine max and glycine soja under salt stress to reveal the phenotypes of their offspring.	metabolomics is developing as an important functional genomics tool for understanding plant systems' response to genetic and environmental changes. here, we characterized the metabolic changes of cultivated soybean c08 (glycine max l. merr) and wild soybean w05 (glycine soja sieb.et zucc.) under salt stress using ms-based metabolomics, in order to reveal the phenotypes of their eight hybrid offspring (9h0086, 9h0124, 9h0391, 9h0736, 9h0380, 9h0400, 9h0434, and 9h0590). total small molecule extra ...	2013	23930713
qtl affecting fitness of hybrids between wild and cultivated soybeans in experimental fields.	the objective of this study was to identify quantitative trait loci (qtl) affecting fitness of hybrids between wild soybean (glycine soja) and cultivated soybean (glycine max). seed dormancy and seed number, both of which are important for fitness, were evaluated by testing artificial hybrids of g. soja × g. max in a multiple-site field trial. generally, the fitness of the f1 hybrids and hybrid derivatives from self-pollination was lower than that of g. soja due to loss of seed dormancy, whereas ...	2013	23919159
a platform for soybean molecular breeding: the utilization of core collections for food security.	soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. china has the largest collection of cultivated soybeans (glycine max) and wild soybeans (glycine soja) all over the world. the platform of soybean core, mini core and integrated applied core collections has been developed in the past decade based on systematic researches which included the sampling strategies, statistical methods, phenotypic data and ssr markers. me ...	2013	23708950
allele-specific marker development and selection efficiencies for both flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase genes in soybean subgenus soja.	color is one of the phenotypic markers mostly used to study soybean (glycine max l. merr.) genetic, molecular and biochemical processes. two p450-dependent mono-oxygenases, flavonoid 3'-hydroxylase (f3'h; ec1.14.3.21) and flavonoid 3',5'-hydroxylase (f3'5'h, ec1.14.13.88), both catalyzing the hydroxylation of the b-ring in flavonoids, play an important role in coloration. previous studies showed that the t locus was a gene encoding f3'h and the w1 locus co-segregated with a gene encoding f3'5'h ...	2013	23463490
ectopic expression of a wrky homolog from glycine soja alters flowering time in arabidopsis.	flowering is a critical event in the life cycle of plants; the wrky-type transcription factors are reported to be involved in many developmental processes sunch as trichome development and epicuticular wax loading, but whether they are involved in flowering time regulation is still unknown. within this study, we provide clear evidence that gswrky20, a member of wrky gene family from wild soybean, is involved in controlling plant flowering time. expression of gswrky20 was abundant in the shoot ti ...	2013	23991184
overexpression of gszfp1 enhances salt and drought tolerance in transgenic alfalfa (medicago sativa l.).	gszfp1 encodes a cys2/his2-type zinc-finger protein. in our previous study, when gszfp1 was heterologously expressed in arabidopsis, the transgenic arabidopsis plants exhibited enhanced drought and cold tolerance. however, it is still unknown whether gszfp1 is also involved in salt stress. gszfp1 is from the wild legume glycine soja. therefore, the aims of this study were to further elucidate the functions of the gszfp1 gene under salt and drought stress in the forage legume alfalfa and to inves ...	2013	23867600
expression of wild soybean wrky20 in arabidopsis enhances drought tolerance and regulates aba signalling.	the wrky-type transcription factors are involved in plant development and stress responses, but how the regulation of stress tolerance is related to plant development is largely unknown. gswrky20 was initially identified as a stress response gene using large-scale glycine soja microarrays. quantitative reverse transcription-pcr (qrt-pcr) showed that the expression of this gene was induced by abscisic acid (aba), salt, cold, and drought. overexpression of gswrky20 in arabidopsis resulted in a dec ...	2013	23606412
a glycine soja aba-responsive receptor-like cytoplasmic kinase, gsrlck, positively controls plant tolerance to salt and drought stresses.	receptor such as protein kinases are proposed to work as sensors to initiate signaling cascades in higher plants. however, little is known about the precise functions of receptor such as protein kinases in abiotic stress response in plants, especially in wild soybean. here, we focused on characterization of the biological functions of a receptor-like cytoplasmic serine/threonine protein kinase gene, gsrlck, which was previously identified as a putative salt-alkali stress-related gene from the tr ...	2013	23494614
physiological mechanisms for high salt tolerance in wild soybean (glycine soja) from yellow river delta, china: photosynthesis, osmotic regulation, ion flux and antioxidant capacity.	glycine soja (bb52) is a wild soybean cultivar grown in coastal saline land in yellow river delta, china. in order to reveal the physiological mechanisms adapting to salinity, we examined photosynthesis, ion flux, antioxidant system and water status in glycine soja under nacl treatments, taking a cultivated soybean, zh13, as control. upon nacl exposure, higher relative water content and water potential were maintained in the leaf of bb52 than zh13, which might depend on the more accumulation of ...	2013	24349468
gssrk, a g-type lectin s-receptor-like serine/threonine protein kinase, is a positive regulator of plant tolerance to salt stress.	receptor-like protein kinases (rlks) play vital roles in sensing outside signals, yet little is known about rlks functions and roles in stress signal perception and transduction in plants, especially in wild soybean. through the microarray analysis, gssrk was identified as an alkaline (nahco3)-responsive gene, and was subsequently isolated from glycine soja by homologous cloning. gssrk encodes a 93.22kda protein with a highly conserved serine/threonine protein kinase catalytic domain, a g-type l ...	2013	23276523
identification of wild soybean mirnas and their target genes responsive to aluminum stress.	micrornas (mirnas) play important regulatory roles in development and stress response in plants. wild soybean (glycine soja) has undergone long-term natural selection and may have evolved special mechanisms to survive stress conditions as a result. however, little information about mirnas especially mirnas responsive to aluminum (al) stress is available in wild soybean.	2012	23040172
over-expression of a novel jaz family gene from glycine soja, increases salt and alkali stress tolerance.	salt and alkali stress are two of the main environmental factors limiting crop production. recent discoveries show that the jaz family encodes plant-specific genes involved in jasmonate signaling. however, there is only limited information about this gene family in abiotic stress response, and in wild soybean (glycine soja), which is a species noted for its tolerance to alkali and salinity. here, we isolated and characterized a novel jaz family gene, gsjaz2, from g. soja. transcript abundance of ...	2012	22943855
gsapk, an aba-activated and calcium-independent snrk2-type kinase from g. soja, mediates the regulation of plant tolerance to salinity and aba stress.	plant snf1 (sucrose non-fermenting-1) related protein kinase (snrk), a subfamily of serine/threonine kinases, has been implicated as a crucial upstream regulator of aba and osmotic signaling as in many other signaling cascades. in this paper, we have isolated a novel plant specific aba activated calcium independent protein kinase (gsapk) from a highly salt tolerant plant, glycine soja (50109), which is a member of the snrk2 family. subcellular localization studies using gfp fusion protein indica ...	2012	22439004
genetic diversity and population structure: implications for conservation of wild soybean (glycine soja sieb. et zucc) based on nuclear and chloroplast microsatellite variation.	wild soybean (glycine soja sieb. et zucc) is the most important germplasm resource for soybean breeding, and is currently subject to habitat loss, fragmentation and population decline. in order to develop successful conservation strategies, a total of 604 wild soybean accessions from 43 locations sampled across its range in china, japan and korea were analyzed using 20 nuclear (nssrs) and five chloroplast microsatellite markers (cpssrs) to reveal its genetic diversity and population structure. r ...	2012	23202917
comparative genomic analysis of soybean flowering genes.	flowering is an important agronomic trait that determines crop yield. soybean is a major oilseed legume crop used for human and animal feed. legumes have unique vegetative and floral complexities. our understanding of the molecular basis of flower initiation and development in legumes is limited. here, we address this by using a computational approach to examine flowering regulatory genes in the soybean genome in comparison to the most studied model plant, arabidopsis. for this comparison, a gen ...	2012	22679494
tracing soybean domestication history: from nucleotide to genome.	since the genome sequences of wild species may provide key information about the genetic elements involved in speciation and domestication, the undomesticated soybean (glycine soja sieb. and zucc.), a wild relative of the current cultivated soybean (g. max), was sequenced. in contrast to the current hypothesis of soybean domestication, which holds that the current cultivated soybean was domesticated from g. soja, our previous work has suggested that soybean was domesticated from the g. soja/g. m ...	2012	23136484
[isolation and functional analysis of gstify11b relevant to salt and alkaline stress from glycine soja].	using homologous cloning and rt-pcr technology, we isolated a novel tify family gene, gstify11b, from glycine soja l. g07256, a species that is tolerant to saline and alkaline environments. phylogenetic analysis indicated that gstify11b was closely related to attify11a with 56% similarity in amino acid identity. protein sequence analysis showed that gstify11b protein also had conserved tify domain, n-terminal domain, and a c-terminal jas motif. quantitative realtime pcr analysis indicated that t ...	2012	22382065
mapping and use of qtls controlling pod dehiscence in soybean.	while the cultivated soybean, glycine max (l.) merr., is more recalcitrant to pod dehiscence (shattering-resistant) than wild soybean, glycine soja sieb. & zucc., there is also significant genetic variation in shattering resistance among cultivated soybean cultivars. to reveal the genetic basis and develop dna markers for pod dehiscence, several research groups have conducted quantitative trait locus (qtl) analysis using segregated populations derived from crosses between g. max accessions or be ...	2012	23136494
genome-wide genetic dissection of germplasm resources and implications for breeding by design in soybean.	"breeding by design" as a concept described by peleman and van der voort aims to bring together superior alleles for all genes of agronomic importance from potential genetic resources. this might be achievable through high-resolution allele detection based on precise qtl (quantitative trait locus/loci) mapping of potential parental resources. the present paper reviews the works at the chinese national center for soybean improvement (ncsi) on exploration of qtl and their superior alleles of agron ...	2012	23136489
polymorphism of dna sequences of cryptochrome genes is not associated with the photoperiodic flowering of wild soybean along a latitudinal cline.	both cultivated soybean and its wild relative glycine soja exhibit strong photoperiodic sensitivity at different latitudes. recent studies have demonstrated that the blue light-absorbing cryptochrome gene, cry1a, is involved in the photoperiodic flowering of soybeans. however, no sequence variation was found in the cdna among cultivars at different latitudinal clines. in the present study, we examined whether positive selection due to polymorphisms in the cryptochrome genes of g. soja occurs. pa ...	2012	22252378
evaluation of soybean germplasm conserved in nias genebank and development of mini core collections.	genetic variation and population structure among 1603 soybean accessions, consisted of 832 japanese landraces, 109 old and 57 recent japanese varieties, 341 landrace from 16 asian countries and 264 wild soybean accessions, were characterized using 191 snp markers. although gene diversity of japanese soybean germplasm was slight lower than that of exotic soybean germplasm, population differentiation and clustering analyses indicated clear genetic differentiation among japanese cultivated soybeans ...	2012	23136496
genetic studies on saline and sodic tolerances in soybean.	salt-affected soils are generally classified into two main categories: saline and sodic (alkaline). developing and using soybean (glycine max (l.) merr) cultivars with high salt tolerance is an effective way of maintaining sustainable production in areas where soybean growth is threatened by salt stress. early classical genetics studies revealed that saline tolerance was conditioned by a single dominant gene. recently, a series of studies consistently revealed a major quantitative trait locus (q ...	2012	23136495
integration of the draft sequence and physical map as a framework for genomic research in soybean (glycine max (l.) merr.) and wild soybean (glycine soja sieb. and zucc.).	soybean is a model for the legume research community because of its importance as a crop, densely populated genetic maps, and the availability of a genome sequence. even though a whole-genome shotgun sequence and bacterial artificial chromosome (bac) libraries are available, a high-resolution, chromosome-based physical map linked to the sequence assemblies is still needed for whole-genome alignments and to facilitate map-based gene cloning. three independent g. max bac libraries combined with ge ...	2012	22413085
genetic analysis of variations in the sugar chain composition at the c-3 position of soybean seed saponins.	saponins are sterols or triterpene glycosides that are widely distributed in plants. the biosynthesis of soybean saponins is thought to involve many kinds of glycosyltransferases, which is reflected in their structural diversity. here, we performed linkage analyses of the sg-3 and sg-4 loci, which may control the sugar chain composition at the c-3 sugar moieties of the soybean saponin aglycones soyasapogenols a and b. the sg-3 locus, which controls the production of group a saponin af, was mappe ...	2012	23136503
pericentromeric effects shape the patterns of divergence, retention, and expression of duplicated genes in the paleopolyploid soybean.	the evolutionary forces that govern the divergence and retention of duplicated genes in polyploids are poorly understood. in this study, we first investigated the rates of nonsynonymous substitution (ka) and the rates of synonymous substitution (ks) for a nearly complete set of genes in the paleopolyploid soybean (glycine max) by comparing the orthologs between soybean and its progenitor species glycine soja and then compared the patterns of gene divergence and expression between pericentromeric ...	2012	22227891
population structure of the wild soybean (glycine soja) in china: implications from microsatellite analyses.	wild soybean (glycine soja), a native species of east asia, is the closest wild relative of the cultivated soybean (g. max) and supplies valuable genetic resources for cultivar breeding. analyses of the genetic variation and population structure of wild soybean are fundamental for effective conservation studies and utilization of this valuable genetic resource.	2012	22791743
recent developments of genomic research in soybean.	soybean is an important cash crop with unique and important traits such as the high seed protein and oil contents, and the ability to perform symbiotic nitrogen fixation. a reference genome of cultivated soybeans was established in 2010, followed by whole-genome re-sequencing of wild and cultivated soybean accessions. these efforts revealed unique features of the soybean genome and helped to understand its evolution. mapping of variations between wild and cultivated soybean genomes were performe ...	2012	22835978
a model to predict the frequency of integration of fitness-related qtls from cultivated to wild soybean.	with the proliferation of genetically modified (gm) products and the almost exponential growth of land use for gm crops, there is a growing need to develop quantitative approaches to estimating the risk of escape of transgenes into wild populations of crop relatives by natural hybridization. we assessed the risk of transgene escape by constructing a population genetic model based on information on fitness-related qtls obtained from an f (2) population of wild soybean g. soja × cultivated soybean ...	2012	21544624
dna sequence polymorphism of the rhg4 candidate gene conferring resistance to soybean cyst nematode in chinese domesticated and wild soybeans.	rhg4 is one of the major resistant genes conferring resistance to soybean cyst nematode races 1, 3 and 4. in order to better understand its sequence diversity among different chinese soybean populations and the impact of human activities on it, we designed 5 primer sets based on its sequence deposited in genbank (genbank accession no. af506518) to obtain the rhg4 sequence from 104 chinese cultivated and wild soybean genotypes, and then analyzed the dna sequence polymorphism in different chinese ...	2012	22924021
over-expression of gszfp1, an aba-responsive c2h2-type zinc finger protein lacking a qalggh motif, reduces aba sensitivity and decreases stomata size.	a cdna of the gene gszfp1 was cloned from glycine soja. gszfp1 encodes a protein with one c2h2-type zinc finger motif. the qalggh motif, which exists in most plant c2h2-type zinc finger proteins (zfps), does not exist in gszfp1. real-time rt-pcr revealed that gszfp1 expression was significantly up-regulated by exogenous aba, both in leaves and roots. over-expression of this gene, in arabidopsis thaliana, resulted in a reduced sensitivity to aba during seed germination and seedling growth. transc ...	2012	22705253
uncovering the salt response of soybean by unraveling its wild and cultivated functional genomes using tag sequencing.	soil salinity has very adverse effects on growth and yield of crop plants. several salt tolerant wild accessions and cultivars are reported in soybean. functional genomes of salt tolerant glycine soja and a salt sensitive genotype of glycine max were investigated to understand the mechanism of salt tolerance in soybean. for this purpose, four libraries were constructed for tag sequencing on illumina platform. we identify around 490 salt responsive genes which included a number of transcription f ...	2012	23209559
the national bioresource project (nbrp) lotus and glycine in japan.	the objective of the national bioresource project (nbrp) in japan is to collect, conserve and distribute biological materials for life sciences research. the project consists of twenty-eight bioresources, including animal, plant, microorganism and dna resources. nbrp lotus and glycine aims to support the development of legume research through the collection, conservation, and distribution of these bioresources. lotus japonicus is a perennial legume that grows naturally throughout japan and is wi ...	2012	23136485
[progress in genes related to seed-coat color in soybean].	seed-coat color has changed from black to yellow during natural and artificial selection of cultivated soybean from wild soybean, and it is also an important morphological marker. therefore, discovering genes related to the soybean seed-coat color will play a very important role in breeding and evolutionary study. different seed-coat colors caused by deposition of various anthocyanin pigments. although pigmentation has been well dissected at molecular level in several plant species, the genes co ...	2012	22698739
genetic diversity of nodulating and non-nodulating rhizobia associated with wild soybean (glycine soja sieb. & zucc.) in different ecoregions of china.	a total of 99 bacterial isolates that originated from root nodules of glycine soja were characterized with restriction analyses of amplified 16s ribosomal dna and 16s-23s rdna intergenic spacers (its), and sequence analyses of 16s rrna, rpob, atpd, reca and nodc genes. when tested for nodulation of g. soja, 72 of the isolates were effective symbionts, and these belonged to five species: bradyrhizobium japonicum, bradyrhizobium elkanii, bradyrhizobium yuanmingense, bradyrhizobium liaoningense and ...	2011	21303397
fullerene-c60 incorporated in liposome exerts persistent hydroxyl radical-scavenging activity and cytoprotection in uva/b-irradiated keratinocytes.	the aim of this study is to examine antioxidant activity of fullerene-c60 (c60) incorporated in liposome (lpsmflln, a diameter of 75.6 nm). lpsmflln is water-soluble, and composed of hydrogenated lecithin of 89.7%, glycine soja sterol of 10% and c60 of 0.3%. hydroxyl radicals (oh), generated from uva- or uvb-irradiated h2o2, were scavenged by lpsmflln but not by c60-lacking lpsm as assessed by esr, showing that the active principle is c60 as scanty as 1/415 weight versus lpsmflln; the oh amoun ...	2011	21780373
The ß-conglycinin deficiency in wild soybean is associated with the tail-to-tail inverted repeat of the a-subunit genes.	ß-Conglycinin, a major seed protein in soybean, is composed of a, a', and ß subunits sharing a high homology among them. Despite its many health benefits, ß-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the a, a', and ß subunits also contain major allergens. A wild soybean (Glycine soja Sieb et Zucc.) line, 'QT2', lacks all of the ß-conglycinin subunits, and the deficien ...	2011	22193750
bradyrhizobium huanghuaihaiense sp. nov., an effective symbiotic bacterium isolated from soybean (glycine max l.) nodules in northern (huang-huai-hai) plain of china.	in a survey of biodiversity and biogeography of rhizobia associated with soybean (glycine max l.) in different sites of northern (huang-huai-hai) plain of china, ten strains were defined as a novel genomic species in the genus of bradyrhizobium. they were distinguished from defined species in rflp (restriction fragment length polymorphism) of 16s rrna gene and intergenic spacer (igs) between 16s and 23s rrna genes. in box-pcr, these strains presented two patterns sharing 94% similarities, demons ...	2011	22003042
phylogenetic relationship of lotus uliginosus symbionts with bradyrhizobia nodulating genistoid legumes.	lotus species are legumes with potential for pastures in soils with low-fertility and environmental constraints. the aim of this work was to characterize bacteria that establish efficient nitrogen-fixing symbiosis with the forage species lotus uliginosus. a total of 39 isolates were obtained from nodules of l. uliginosus naturally growing in two different locations of portugal. molecular identification of the isolates plus the commercial inoculant strain nzp2039 was performed by rep-pcr, 16s rrn ...	2011	22092879
gstify10, a novel positive regulator of plant tolerance to bicarbonate stress and a repressor of jasmonate signaling.	recent discoveries show that tify family genes are plant-specific genes involved in the response to several abiotic stresses, also acting as key regulators of jasmonate signaling in arabidopsis thaliana. however, there is limited information about this gene family in wild soybean, nor is its role in plant bicarbonate stress adaptation completely understood. here, we isolated and characterized a novel tify family gene, gstify10, from glycine soja. gstify10 could be induced by bicarbonate, salinit ...	2011	21805375
a novel glycine soja tonoplast intrinsic protein gene responds to abiotic stress and depresses salt and dehydration tolerance in transgenic arabidopsis thaliana.	tonoplast intrinsic protein (tip) is a subfamily of the aquaporin (aqp), also known as major intrinsic protein (mip) family, and regulates water movement across vacuolar membranes. some reports have implied that tip genes are associated with plant tolerance to some abiotic stresses that cause water loss, such as drought and high salinity. in our previous work, we found that an expressed sequence tag (est) representing a tip gene in our glycine soja est library was inducible by abiotic stresses. ...	2011	21397356
alkaline-stress response in glycine soja leaf identifies specific transcription factors and aba-mediated signaling factors.	transcriptome of glycine soja leaf tissue during a detailed time course formed a foundation for examining transcriptional processes during nahco(3) stress treatment. of a total of 2,310 detected differentially expressed genes, 1,664 genes were upregulated and 1,704 genes were downregulated at various time points. the number of stress-regulated genes increased dramatically after a 6-h stress treatment. go category gene enrichment analysis revealed that most of the differentially expressed genes w ...	2011	20938706
[study of interaction of wild soybean subpopulations (glycine soja) in the valley of the tsukanovka river in the south of far east of russia].	a comparative study of the genetic structure of natural and anthropogenic populations of g. soja gives significant information about formation of different populations, and allows developing measures for preservation of unique natural gene bank of wild soybean, the species closely related to cultivated soybean. in this study, issr markers were used to carry out a comparative analysis of genetic structure of natural and anthropogenic subpopulations of g. soja for studying possible mutual influenc ...	2011	21950138
gsgasa1 mediated root growth inhibition in response to chronic cold stress is marked by the accumulation of dellas.	low, but not freezing temperatures are a major factor limiting plant vegetative growth and development. it is not clear what signaling mechanism is used to limit plant growth in response to chronic low temperatures, thus it is important to continue to isolate and characterize genes whose activity/expression correlate well with cold responses. in this study, a novel gasa gene was isolated from glycine soja and named gsgasa1. quantitative real-time pcr analysis indicated that gsgasa1 expression re ...	2011	21855169
secondary metabolites from glycine soja and their growth inhibitory effect against spodoptera litura.	the wild soybean (glycine soja sieb. et zucc) has been reported to be relatively resistant to insect and pathogenic pests. however, the responsible secondary metabolites in the aerial part of this important plant are largely unknown. from the aerial part of g. soja, 13 compounds were isolated and identified, including seven isoflavonoids (1-7), a cyclitol (8), two sterol derivatives (9 and 10), and three triterpenoids (11-13). compound 7 is a new isoflavonoid, and compounds 9 and 10 are reported ...	2011	21545185
[study of genetic diversity and spatial structure of the wild soybean (glycine soja sieb. & zucc.) population from the ekaterinovka in the south of primorskii krai].	data are presented on the genetic diversity and spatial structure of the natural wild soybean population from the neighborhood of the settlement of ekaterinovka in primorskii krai and on the relationship between the genetic structure of this population and its spatial organization. these data are discussed in comparison with the results of studies of wild soybean populations in the far east region of the russian federation and china. recommendations are given concerning the collection of genetic ...	2011	21542304
treatment of bromhidrosis with a glycine-soja sterocomplex topical product.	bromhidrosis is a common problem with a severe negative impact on the patient's social and emotional life. the objective of this open-label study was to evaluate the efficacy and safety of a local product with the main active ingredient of glycine-soja sterocomplex in the treatment of local bromhidrosis.	2011	21332920
gscbrlk, a calcium/calmodulin-binding receptor-like kinase, is a positive regulator of plant tolerance to salt and aba stress.	calcium/calmodulin-dependent kinases play vital roles in protein phosphorylation in eukaryotes, yet little is known about the phosphorylation process of calcium/calmodulin-dependent protein kinase and its role in stress signal transduction in plants. a novel plant-specific calcium-dependent calmodulin-binding receptor-like kinase (gscbrlk) has been isolated from glycine soja. a subcellular localization study using gfp fusion protein indicated that gscbrlk is localized in the plasma membrane. bin ...	2010	20400529
phenolics in the seed coat of wild soybean (glycine soja) and their significance for seed hardness and seed germination.	hardseededness in annual wild soybean (glycine soja sieb. et zucc.) is a valuable trait that affects the germination, viability, and quality of stored seeds. two g. soja ecotypes native to shandong province of china have been used to identify the phenolics in the seed coat that correlate with the seed hardness and seed germination. three major phenolics from the seed coat were isolated and identified as epicatechin, cyanidin 3-o-glucoside, and delphinidin 3-o-glucoside. of the three phenolics, o ...	2010	20879792
a new allele of flower color gene w1 encoding flavonoid 3'5'-hydroxylase is responsible for light purple flowers in wild soybean glycine soja.	glycine soja is a wild relative of soybean that has purple flowers. no flower color variant of glycine soja has been found in the natural habitat.	2010	20663233
global transcriptome profiling of wild soybean (glycine soja) roots under nahco3 treatment.	plant roots are the primary site of perception and injury for saline-alkaline stress. the current knowledge of saline-alkaline stress transcriptome is mostly focused on saline (nacl) stress and only limited information on alkaline (nahco3) stress is available.	2010	20653984
whole-genome sequencing and intensive analysis of the undomesticated soybean (glycine soja sieb. and zucc.) genome.	the genome of soybean (glycine max), a commercially important crop, has recently been sequenced and is one of six crop species to have been sequenced. here we report the genome sequence of g. soja, the undomesticated ancestor of g. max (in particular, g. soja var. it182932). the 48.8-gb illumina genome analyzer (illumina-ga) short dna reads were aligned to the g. max reference genome and a consensus was determined for g. soja. this consensus sequence spanned 915.4 mb, representing a coverage of ...	2010	21131573
hybridization between gm soybean (glycine max (l.) merr.) and wild soybean (glycine soja sieb. et zucc.) under field conditions in japan.	accumulation of information about natural hybridization between gm soybean (glycine max) and wild soybean (glycine soja) is required for risk assessment evaluation and to establish biosafety regulations in japan. this is particularly important in areas where wild relatives of cultivated soybean are grown (i.e. east asia including japan). to collect information on temporal and spatial factors affecting variation in hybridization between wild and gm soybean, a two year hybridization experiment was ...	2010	21122483
resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection.	we report a large-scale analysis of the patterns of genome-wide genetic variation in soybeans. we re-sequenced a total of 17 wild and 14 cultivated soybean genomes to an average of approximately ×5 depth and >90% coverage using the illumina genome analyzer ii platform. we compared the patterns of genetic variation between wild and cultivated soybeans and identified higher allelic diversity in wild soybeans. we identified a high level of linkage disequilibrium in the soybean genome, suggesting th ...	2010	21076406
natural variability in abundance of prevalent soybean proteins.	soybean is an inexpensive source of protein for humans and animals. genetic modifications (gmo) to soybean have become inevitable on two fronts, both quality and yield will need to improve to meet increasing global demand. to ensure the safety of the crop for consumers it is important to determine the natural variation in seed protein constituents as well as any unintended changes that may occur in the gmo as a result of genetic modification. understanding the natural variation of seed proteins ...	2010	20709130
genetic diversity in domesticated soybean (glycine max) and its wild progenitor (glycine soja) for simple sequence repeat and single-nucleotide polymorphism loci.	• the study of genetic diversity between a crop and its wild relatives may yield fundamental insights into evolutionary history and the process of domestication. • in this study, we genotyped a sample of 303 accessions of domesticated soybean (glycine max) and its wild progenitor glycine soja with 99 microsatellite markers and 554 single-nucleotide polymorphism (snp) markers. • the simple sequence repeat (ssr) loci averaged 21.5 alleles per locus and overall nei's gene diversity of 0.77. the snp ...	2010	20618914