Publications

constancy of nitrogen turnover kinetics in the plant cell: insights into the integration of subcellular n fluxes.	compartmental analysis with 13n was used to determine cytosolic nitrate (no3-) pools, and their turnover rates, in roots of intact barley (hordeum vulgare l. cv klondike) seedlings. influx, efflux, flux to the vacuole and assimilation, and flux to the xylem, varied as much as 300-fold over a wide range of external no3- conditions. by contrast, the kinetic constant kc describing cytosolic no3- turnover varied by less than 4% from a mean value of 0.0407 min(-1). accordingly, cytosolic no3- pools v ...	2001	11469581
germination, growth and gas exchange of selected boreal forest seedlings in soil containing oil sands tailings.	greenhouse experiments were conducted to determine the effects of soil enriched in fine tailings (ft), produced by the oil sands extraction, on germination, seedling growth and physiology of several plant species of the boreal forest. the germination of seeds was initially delayed by 15% ft in dogwood (cornus stolonifera michx) and jack pine (pinus banksiana lamb) but not in white spruce [picea glauca (moench) voss]. in the second set of experiments we showed that all dogwood seedlings survived ...	2000	15092982
changes in volatile terpene and diterpene resin acid composition of resistant and susceptible white spruce leaders exposed to simulated white pine weevil damage.	induced (traumatic) resin in white spruce (picea glauca (moench) voss) leaders resistant or susceptible to the white pine weevil (pissodes strobi peck) was analyzed for volatile terpenes and diterpene resin acids after simulated white pine weevil damage. leaders from 331 trees were wounded just below the apical bud with a 1-mm diameter drill, coinciding with the natural time of weevil oviposition in the spring. leaders were removed in the fall, and the bark and xylem from the upper and lower reg ...	2000	11269960
reduction in turgid water volume in jack pine, white spruce and black spruce in response to drought and paclobutrazol.	significant reductions in needle water content were observed in white spruce (picea glauca (moench) voss), black spruce (picea mariana (mill) b.s.p.), and jack pine (pinus banksiana lamb.) seedlings in response to a 10-day drought, although turgor was apparently maintained. when the seedlings were re-watered after the drought, jack pine needles regained their original saturated volume, whereas white spruce and black spruce needles did not. significant drought-induced reductions in turgor-loss vo ...	2000	12651520
two highly divergent 5s rdna unit size classes occur in composite tandem array in european larch (larix decidua mill.) and japanese larch (larix kaempferi (lamb.) carr.).	the 5s ribosomal dna unit structure and organization have been investigated in larix decidua and larix kaempferi using selective amplification of gene and spacer, sequence analysis and homologous probe hybridization. two highly divergent unit size classes of approximately 650 and 870 bp were detected in both species. sequence analysis in larix decidua revealed that length variations occur in the middle spacer region and are the result of duplications (in the long spacers) and considerable sequen ...	1999	10584306
tetranorditerpene lactones, potent antifungal antibiotics for human pathogenic yeasts, from a unique species of oidiodendron.	the culture filtrate of a fungus isolated from decaying picea glauca wood and tentatively identified as oidiodendron cf. truncatum showed strong antibiotic activity against the pathogenic yeast, candida albicans. four new tetranorditerpenoids, oidiodendrolides a (3), b (4), and c (5) and oidiodendronic acid (7) were isolated along with three known tetranorditerpenoids, ll-z1271 alpha (= pr1387) (1), pr1388 (2), and acrostalidic acid (6), from rice fermented by the above fungus. the structures of ...	1999	10605057
seasonal photosynthetic responses to light and temperature in white spruce (picea glauca) seedlings planted under an aspen (populus tremuloides) canopy and in the open.	photosynthetic light and temperature response curves were measured seasonally in seedlings of white spruce (picea glauca (moench.) voss) grown for two years in the understory of aspen (populus tremuloides michx.) or in the open in central alberta. light-saturated rate of net photosynthesis, the optimum temperature for net photosynthesis, transpiration rate, photochemical efficiency, and stomatal and mesophyll conductances increased from spring to summer and declined thereafter, whereas dark resp ...	1997	14759835
carrier herbal medicine: traditional and contemporary plant use.	the carrier, an athapaskan-speaking people of northcentral british columbia, occupy the sub-boreal spruce forests of the central interior. this report, which is based on field study, documents some traditional and contemporary knowledge of the medicinal use of plants by the carrier people. important medicinal plants include: abies lasiocarpa, alnus incana, arctostaphylos uva-ursi, artemisia frigida, fragaria virginiana, juniperus communis, picea glauca, pinus contorta, populus tremuloides, rubus ...	1996	8735452
carrier herbal medicine: an evaluation of the antimicrobial and anticancer activity in some frequently used remedies.	the antimicrobial properties of some traditional carrier herbal preparations were evaluated using an agar dilution method. pitch preparations were screened against known human pathogens: escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, candida albicans and aspergillus fumigatus. the results indicated definite antimicrobial activity in the pitch preparations of picea glauca and pinus contorta and provide a starting point for pharmacognostic evaluation of these species. in addition ...	1996	8771456
polyamine biosynthesis during somatic embryogenesis in interior spruce (picea glauca x picea engelmannii complex).	putrescine, spermidine, and spermine levels during somatic embryogenesis of interior spruce (picea glauca x picea engelmannii complex) were quantified on abscisic acid supplemented growth medium putrescine and spermidine levels increased two-fold coinciding with maturation of the early somatic embryos to globular embryos. polyclonal antibodies raised against escherichia coli arginine decarboxylase (adc) and ornithine decarboxylase (odc), following affinity purification specifically recognized sp ...	1996	24178460
pollen management for intensive seed orchard production.	artificially increasing pollen supply (supplemental mass pollination (smp)) to conifer seed orchards has the potential to increase seed yields and improve the genetic worth of seed crops that would otherwise suffer from the detrimental effects of pollen contamination and unbalanced paternal contribution. however, success rates, measured as the proportion of seed fertilized by smp, have been low. this review examines the concepts underlying smp and presents data for two smp field trials with doug ...	1995	14965935
seasonal nutrient dynamics in white pine and white spruce in response to environmental manipulation.	seasonal retranslocation in white pine (pinus strobus l.) and white spruce (picea glauca (moench) voss) was examined in response to silvicultural treatments (scarification, annual fertilization application, and annual control of competing vegetation with herbicide) that changed both environmental conditions and the growth rate of the trees. four years after plantation establishment and initial treatment, nutrient accumulation in current-year needles of white pine and retranslocation from 1-year- ...	1995	14965969
effect of photon flux density on carbon assimilation and chlorophyll a fluorescence of cold-stored white spruce and lodgepole pine seedlings.	white spruce (picea glauca (moench.) voss) and lodgepole pine (pinus contorta dougl. var. latifolia engelm.) seedlings previously held in dark, frozen storage (-2 degrees c) for 2.5 or 6 months, and nursery-grown white spruce seedlings lifted in summer were exposed to photon flux densities (pfds) similar to those that might be encountered at planting. photosynthetic gas exchange and chlorophyll a (chl a) fluorescence were examined in cold-stored and summer-lifted seedlings before and after a 9 h ...	1993	14969928
inheritance of mitochondrial dna in the conifer larix.	restriction fragment length polymorphisms between larix leptolepis and larix decidua were identified in heterologous hybridization experiments, using wheat mitochondrial dna probes specific for atp9, coxi, nad3/rps12, and orf25. analysis of eight individuals of each reciprocal hybrid of these two species revealed that mitochondrial dna was maternally inherited. furthermore, sequences homologous to wheat orf25 were also identified in larix gmelini, larix siberica, larix olgensis, and larix larici ...	1993	24193487
acquired thermotolerance of jack pine, white spruce and black spruce seedlings.	the acquired thermotolerance of first-year seedlings of jack pine (pinus banksiana lamb.) hardened at 36, 38, 40 or 42 degrees c for 90, 180 or 360 minutes and of black spruce (picea mariana (mill.) b.s.p.) hardened at 34, 36, 38 or 40 degrees c for 30, 90, 180 or 360 minutes was determined by comparison of needle damage to that of non-hardened seedlings (25 degrees c) following exposure to temperatures of 49 and 47.5 degrees c, respectively. compared to seedlings kept at 25 degrees c, heat inju ...	1991	14972899
accumulation of free amino acids in the shoots and roots of three northern conifers during drought.	the effects of drought on the free amino acid pools in 21- to 23-week-old seedlings of black spruce (picea mariana (mill.) britt.), white spruce (picea glauca (moench.) voss) and jack pine (pinus banksiana lamb.) were followed during soil drying. although water and pressure potentials were sensitive to water deficits, large changes in osmotic potential were not recorded until after the development of severe drought. total soluble amino nitrogen in the shoots and roots of the three species rose a ...	1990	14972940
the effects of promoter on transient expression in conifer cell lines.	protoplasts from suspension cultures of somatic embryos of white spruce (picea glauca moench voss) were electroporated with plasmids containing the chimeric genes for chloramphenicol acetyl transferase (cat) or β-glucuronidase (gus), under control of one of three promoters. transient cat gene expression of approximately equal magnitude resulted when the cat gene was fused to either the cauliflower mosaic virus (camv) 35s promoter or the nopaline synthase (nos) promoter. when the cat gene was fus ...	1990	24226354
peg-mediated expression of gus and cat genes in protoplasts from embryogenic suspension cultures of picea glauca.	ß-glucuronidase (gus) and chloramphenicol acetyl transferase (cat) were used as reporter proteins in protoplasts from embryogenic suspension cultures of picea glauca (moench) voss (white spruce). plasmid dna enclosing chimeric gus and cat constructs, using the cauliflower mosaic virus 35s promoter, was introduced into picea glauca protoplasts using polyethylene glycol (peg). transient expression was detected 12 to 40 h after peg-mediated dna delivery. dose-response curves using covalently closed ...	1989	24240467
transient gene expression in electroporated picea glauca protoplasts.	the reporter gene for chloramphenicol acetyltransferase (cat) was introduced into white spruce (picea glauca (moench) voss.) protoplasts by electroporation. cat transient gene expression was increased by increasing the concentration of pcamvcn plasmid and was affected by the level of the applied voltage. highest cat activities were obtained after electroporation with a pulse of 350v.cm(-1) having an exponential decay constant of approximately 105ms. linearized plasmid constructs gave much higher ...	1988	24240396
planting stress in newly planted jack pine and white spruce. 1. factors influencing water uptake.	bareroot jack pine (pinus banksiana lamb.) seedlings (2 + 0) and bareroot white spruce (picea glauca (moench) voss) transplants (1 1/2 + 1 1/2) were taken from cold storage and planted on a clearcut forest site in northeastern ontario on several dates between may 6 and june 5 during which period soil temperature at 15 cm depth increased from 0 to 18 degrees c. additional cold-stored trees were transferred to a greenhouse where they were grown in pots for 0, 7 or 28 days and then placed with thei ...	1988	14972837
planting stress in newly planted jack pine and white spruce. 2. changes in tissue water potential components.	water relations of bare-root jack pine (pinus banksiana lamb.) and white spruce (picea glauca (moench) voss) planted in a greenhouse and on a boreal cut-over site were examined during the first growing season. in field-planted trees, maximum stomatal conductances (g(wv)) were initially low (< 0.10 cm s(-1)). base and minimum xylem pressure potentials (psi(x(base)) and psi(x(min))) were less than -1.5 and -1.7 mpa for jack pine and -2.0 and -2.6 mpa for white spruce, respectively. during the grow ...	1988	14972838
effects of carbon dioxide enrichment and nitrogen supply on growth of boreal tree seedlings.	the effects of two levels of atmospheric carbon dioxide (350 microl l(-1), 750 microl l(-1)) and three levels of nitrogen (15.5 mm, 1.55 mm, 0.155 mm n) on biomass accumulation and partitioning were examined in aspen (populus tremuloides michx.) and white spruce (picea glauca (moench) voss) seedlings grown in controlled environment rooms for 100 days after germination. nitrogen supply had pronounced effects on biomass accumulation, height, and leaf area of both species. root weight ratio (rwr) o ...	1986	14975856
relationships among drought resistance, transpiration rates, and abscisic acid levels in three northern conifers.	water potentials, transpiration rates and abscisic acid (aba) levels in shoots of black spruce (picea mariana (mill.) bsp), white spruce (picea glauca (moench) voss) and jack pine (pinus banksiana lamb.) seedlings were monitored during periods of drought and recovery from drought. abscisic acid contents of shoots increased during the period of drought as water potentials decreased. the increase in levels of aba was closely associated with a decrease in rates of transpiration. in the spruces, the ...	1986	14975892
some phytotoxic effects of fenitrothion on the germination and early seedling growth of picea glauca (moench) voss and betula alleghaniensis britton.		1979	486750