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confirmation by gas chromatography/mass spectrometry of two unusual trans-3-monoethylenic fatty acids from the nova scotian seaweeds palmaria palmata and chondrus crispus.the structures of two unusual fatty acids, the known trans-3-hexadecenoic acid and a novel trans-3-tetradecenoic acid, both isolated from the nova scotian seaweeds palmaria palmata and chondrus crispus, were positively identified. after the extraction of the total fatty acids by saponification, followed by methylation, the monoenoic trans fractions were isolated by thin-layer chromatography on silica gel impregnated with silver nitrate. the monoenoic trans fractions were derivatized with 2-amino ...19948090067
the gapdh gene system of the red alga chondrus crispus: promoter structures, intron/exon organization, genomic complexity and differential expression of genes.our previous phylogenetic analysis based on cdna sequences of chloroplast and cytosolic glyceraldehyde-3-phosphate dehydrogenases (gapdh; genes gapa and gapc, respectively) of the red alga chondrus crispus suggested that rhodophytes and green plants are sister groups with respect to plastids and mitochondria and diverged at about the same time or somewhat later than animals and fungi. here we characterize the genomic sequences of genes gapc and gapa of c. crispus with respect to promotor structu ...19938260635
structure of the oxidized long-chain flavodoxin from anabaena 7120 at 2 a resolution.the structure of the long-chain flavodoxin from the photosynthetic cyanobacterium anabaena 7120 has been determined at 2 a resolution by the molecular replacement method using the atomic coordinates of the long-chain flavodoxin from anacystis nidulans. the structure of a third long-chain flavodoxin from chondrus crispus has recently been reported. crystals of oxidized a. 7120 flavodoxin belong to the monoclinic space group p2(1) with a = 48.0, b = 32.0, c = 51.6 a, and beta = 92 degrees, and one ...19921303762
crystal structure of oxidized flavodoxin from a red alga chondrus crispus refined at 1.8 a resolution. description of the flavin mononucleotide binding site.in order to describe the detailed conformation of the oxidized flavodoxin from a eukaryotic red alga, chondrus crispus, the crystal structure has been refined by a restrained least-squares method. the crystallographic r factor is 0.168 for 13,899 reflections with f greater than 2 sigma f between 6.0 and 1.8 a resolution. the refined model includes 173 amino acid residues, flavin mononucleotide (fmn) and 110 water molecules. the root-mean-square deviation in bond lengths from ideal values is 0.01 ...19921602481
protoplast production in chondrus crispus gametophytes (gigartinales, rhodophyta).protoplasts were isolated from female gametophytes of chondrus crispus (stackh.) using commercial cellulase and various carrageenases prepared from marine bacteria. depending on the nature of the donor tissue (apices or whole thallus, wild or cultivated strains), yields ranged from 1.0-8.5×10(8) protoplasts per gram of fresh tissue. preincubating the tissue with a potassium chelator, kryptofix 222, enhanced protoplast yields by 30-50 %. based on staining with fluorescein diacetate most protoplas ...199024232676
tertiary structure of oxidized flavodoxin from an eukaryotic red alga chondrus crispus at 2.35-a resolution. localization of charged residues and implication for interaction with electron transfer partners.the crystal structure of the oxidized form of a flavodoxin from an eukaryotic red alga, chondrus crispus, has been determined by multiple isomorphous replacement and anomalous scattering methods. a model of the 173 residues and flavin mononucleotide (fmn) has been refined by a restrained least squares method to a crystallographic r-factor of 22.6% using 6236 reflections between 6.0 and 2.35 a with f greater than 3 sigma f. this molecule has a sheet consisting of five parallel beta-strands with t ...19902394748
distribution of carbonic anhydrase in british marine macroalgae.thirty-four species of marine macroalgae from around st. andrews, scotland, have been assayed for their external activity and thirty-three species for their total activity of carbonic anhydrase. activity was detected in all the rhodophyta tested apart from chondrus crispus, but was absent in codium fragile, enteromorpha sp. and monostroma fuscum (chlorophyta), and alaria esculenta, laminaria digitata, l. saccharina and l. hyperborea (phaeophyta). total activity of carbonic anhydrase per unit fre ...198928312650
the amino acid sequence of a flavodoxin from the eukaryotic red alga chondrus crispus.the amino acid sequence of the constitutive flavodoxin from the red alga chondrus crispus was determined from the analyses of peptide fragments derived by enzymic digestions of the carboxymethylated protein. this is the first sequence reported for a flavodoxin from a eukaryote. the protein is composed of 173 amino acid residues and is a member of the longer-chain group of flavodoxins. the extent of sequence homology to the three other flavodoxins in the group for which sequences are available is ...19892597140
crystallization and preliminary x-ray diffraction studies of oxidized flavodoxin from chondrus crispus, a red alga.crystals of the oxidized form of flavodoxin from a red alga, chondrus crispus, have been grown in ammonium sulfate solution by the dialysis method. the crystals belong to the orthorhombic system, space group p2(1)2(1)2(1), with unit cell dimensions of a = 63.6, b = 48.8, and c = 56.8 a. the asymmetric unit contains one molecule of flavodoxin. the crystals diffract x-rays to about 2.0 a resolution and are stable to x-ray beams. the diffraction patterns changed significantly upon soaking the cryst ...19892732209
mechanism of photosynthetic carbon dioxide uptake by the red macroalga, chondrus crispus.the aim of this study was to determine how chondrus crispus, a marine red macroalga, acquires the inorganic carbon (c(i)) it utilizes for photosynthetic carbon fixation. analyses of c(i) uptake were done using silicone oil centrifugation (using multicellular fragments of thallus), infrared gas analysis, and gas chromatography. inhibitors of carbonic anhydrase (ca), the band 3 anion exchange protein and na(+)/k(+) exchange were used in the study. it was found that: (a) c. crispus does not accumul ...198916666552
carbonic anhydrase-dependent inorganic carbon uptake by the red macroalga, chondrus crispus.the rate of photosynthetic carbon uptake of chondrus crispus stack-house plants, at various co(2) concentrations and pretreated with carbonic anhydrase (ca) inhibitors, was determined using an air-suspension, differential infra-red gas analyzer technique. it was found that the ca inhibitors, acetazolamide, dextran-bound acetazolamide (dbi, which does not permeate cell membranes), and subtilisin (a protease that attacks the cell surface) inhibit photosynthetic carbon uptake in c. crispus. inhibit ...198716665329
on the nature of the oxygen uptake in the light by chondrus crispus. effects of inhibitors, temperature and light intensity.the nature of the different processes of o2 uptake involved in the light in the red macroalga chondrus crispus stackhouse (rhodophyta, gigartinales) was investigated. at limiting co2, inh (2.5 mm) did not alter the o2 uptake rate. glycolate was not excreted and did not accumulate within the cells. kcn reduced the rate of o2 uptake in the light by 76% at limiting co2 and by 43% at saturating co2, but caused > 95% inhibition of o2 evolution. dcmu (5 μm) totally blocked the photosynthetic electron ...198724435462
[a potent inhibitor of bacterial growth from a seaweed, chondrus crispus]. 19863467264
dark respiration in the marine macroalga chondrus crispus (rhodophyceae).dark respiration in the red macroalga chondrus crispus was studied under a variety of conditions. the components of respiration were examined using selective inhibitors in order to characterise pathways of respiration and examine regulation of respiration in marine macroalgae.in comparison to respiration rates generally reported for higher-plant leaves and roots, the steady-state rate of o2 consumption by this alga, after 30 min dark pretreatment, was found to be quite low (three- to sixfold low ...198624232031
preferential photosynthetic uptake of exogenous hco(3) in the marine macroalga chondrus crispus.the rate of hco(3) (-) uptake by the red macroalga chondrus crispus has been investigated. unbalanced concentrations of free co(2) and hco(3) (-), generated by the photosynthetic activity, were detected in steady state conditions by using an exchange column apparatus linked to an assimilation chamber. observing the variations of this gradient as influenced by the time of seawater transit from the assimilation chamber towards the column allowed an experimental determination of: (a) the actual gra ...198616664721
continuous measurements of the free dissolved co(2) concentration during photosynthesis of marine plants: evidence for hco(3) use in chondrus crispus.an experimental system consisting of a gas exchange column linked to an assimilation chamber has been developed to record continuously the free dissolved co(2) concentration in seawater containing marine plants. from experiments performed on the red macroalga chondrus crispus (rhodophyta, gigartinales), this measurement is in agreement with the free co(2) concentration calculated from the resistance to co(2) exchanges in a biphasic system (gas and liquid) as earlier reported. the response time o ...198516664281
oxygen uptake and photosynthesis of the red macroalga, chondrus crispus, in seawater: effects of oxygen concentration.with an experimental system developed for aquatic plants using the mass spectrometry technique and infrared gas analysis of co(2), we studied the responses to various o(2) concentrations of gas exchanges with the red macroalga chondrus crispus s. the results were as follows. (a) irrespective of the co(2) concentration, net photosynthesis was o(2) sensitive with a 45 to 70% stimulation at 2% o(2). even with high co(2), a significant warburg effect was detected. (b) although photosynthesis was co( ...198516664280
oxygen uptake and photosynthesis of the red macroalga, chondrus crispus, in seawater: effects of light and co(2) concentration.with an experimental system using mass spectrometry techniques and infra-red gas analysis of co(2) developed for aquatic plants, we studied the responses to various light intensities and co(2) concentrations of photosynthesis and o(2) uptake of the red macroalga chondrus crispus s. the co(2) exchange resistance at air-water interface which could limit the photosynthesis was experimentally measured. it allowed the calculation of the free dissolved co(2) concentration. the response to light showed ...198416663760
redox potentials of algal and cyanobacterial flavodoxins.the redox potentials of flavodoxins from the cyanobacteria synechococcus pcc 6301 (formerly anacystis nidulans) and nostoc strain mac, and from the red alga chondrus crispus, were determined by potentiometric titration. for the oxidized-semiquinone interconversion the potentials at ph 7.0 of the three flavodoxins were between -210 and -235 mv, and these were ph-dependent over the range ph 6.9-8.2. for the semiquinone-reduced interconversion the potentials of the cyanobacterial flavodoxins were c ...19846424653
components of predation intensity in the low zone of the new england rocky intertidal region.predation intensity often increases along local gradients of decreasing environmental rigor. assuming factors such as productivity and heterogeneity do not change along such gradients, potential factors responsible for this pattern include: (1) increased effectiveness per individual predator, (2) increased numbers of individual predators, (3) increased numbers of predator species of the same general morphological attributes, and (4) increased numbers of predator species of different general morp ...198328310571
apoflavodoxin aggregation following dissociation of flavin.flavodoxins were isolated for the cyanobacteria anacystis nidulans and nostoc strain mac, and from the red alga chondrus crispus, and apoflavodoxins prepared by five methods. gel electrophoretic studies showed that whereas the apoproteins of a. nudulans and nostoc strain mac were recovered in monomeric form, the removal of riboflavin 5'-phosphate from c. crispus flavodoxin resulted in extensive aggregation of the apoprotein. in extent and nature this aggregation differed with the dissociating ag ...19806774761
efficiency of ferredoxins and flavodoxins as mediators in systems for hydrogen evolution.1. the efficiencies of ferredoxins and flavodoxins from a range of sources as mediators in systems for hydrogen evolution were assessed. 2. in supporting electron transfer from dithionite to hydrogenase of the bacterium clostridium pasteurianum, highest activity was shown by the ferredoxin from the cyanobacterium chlorogloeopsis fritschii and flavodoxin from the bacterium megasphaera elsdenii. the latter was some twenty times as active as comparable concentrations of methyl viologen. ferredoxins ...19807016115
glycosulphatase from pseudomonas carrageenovora. purification and some properties.a glycosulphatase present in the soluble fraction of disrupted pseudomonas carrageenovora has been purified 500-fold by gel filtration on sephacryl s-200 and ion-exchange chromatography on deae-sepharose cl-6b. by dodecylsulphate/polyacrylamide gel electrophoresis the enzyme is practically homogeneous and has a molecular weight of 55 000. conditions of optimal sodium chloride concentration and ph at 25 degrees c were 0.25--0.50 mol dm-3 and ph 7.0 respectively. the purified enzyme was inhibited ...1979520310
incorporation of [s]sulfate and [c]bicarbonate into karyotype-specific polysaccharides of chondrus crispus.gametophytic and sporophytic tissues of chondrus crispus (stackhouse) cultured in vitro were labeled with (35)s and (14)c. the major sulfated polysccharides isolated from the two karyotypes were characterized by kci fractionation, immunoprecipitation, and infrared spectroscopy. reproducibility of data has been demonstrated by an experiment using gametophytic t(4) strain with five replicates per time point. the rate of sulfate uptake was similar in haploid and diploid plants from a given area cul ...197916661014
quantitative x-ray microanalysis of halogen elements in biological specimens.standards were prepared for quantitative x-ray microanalysis of the halogen elements cl, br and i in sections of resin embedded biological specimens. halogenated aromatic compounds were dissolved in resin, which subsequently was polymerized. homogeneity and stability of the standards were determined and found to be satisfactory. a general procedure of calculation of elemental concentrations according to the continuum method is given for the case that the specimen contains appreciable concentrati ...1979521320
sulfohydrolase activity and carrageenan biosynthesis in chondrus crispus (rhodophyceae).an enzyme catalyzing the conversion of mu- to kappa-carrageenan has been demonstrated in both haploid and diploid plants of chondrus crispus. it acts at the polymer level producing 3,6-anhydro-d-galactose with the stoichiometric release of sulfate. two-thirds of the recoverable enzyme was associated with the 15,000g pellet most of which could be solubilized by passage through a ribi cell fractionator. the enzyme precipitated between 2.65 and 4.24 m (nh(4))(2)so(4) and was partly purified on deae ...197816660358
the immunochemistry of lambda-type carrageenans from certain red algae.an antibody preparation directed against a structural feature associated with 6-sulphate groups was used to probe structural relations among certain lambda-type carrageenans. immunochemical and chemical differences are described between the kc1-soluble carrageenans from tetrasporic algal plants of gigartina corymbifera, gigartina sp. from san francisco bay, petrocelis middendorfii, iridaea cordata, rhodoglossum californicum, and chondrus crispus. the differences in immunochemical reactivity of t ...1978568513
the occurrence and seasonal variation of gigartinine and l-citrullinyl-l-arginine in chondrus crispus stackh.gigartinine, 5-(3-amidinoureido)-2-aminovaleric acid, and l-citrullinyl-l-arginine were islated from aqueous extracts of chondrus crispus (rhodophyceae). their identifications were confirmed by chemical procedures, and 1h and 13c nuclear magnetic resonance and infrared spectroscopic methods. citrullinylarginine, gigartinine, taurine, citrulline, and glutamic acid were the predominant free amino compounds. citrullinylarginine showed the most pronounced change in concentration. this occurred durin ...1977837242
h(2) metabolism in photosynthetic organisms: i. dark h(2) evolution and uptake by algae and mosses.dark h(2) metabolism was studied in marine and fresh water red algae, the green alga, chlamydomonas, and mosses. a time variable and temperature-sensitive anaerobic incubation was required prior to h(2) evolution. h(2) evolution was sensitive to disalicylidenepropanediamine. an immediate h(2) uptake was observed in these algae. immediate dark h(2) uptake but no evolution was observed in the mosses.a cell-free hydrogenase preparation was obtained from anaerobically adapted chlamydomonas reinhardi ...197516659260
carrageenans in the gametophytic and sporophytic stages of chondrus crispus.the morphologically similar sporophytic and gametophytic plants of chondrus crispus stackhouse were examined and it was shown that the former contain λ-carrageenan. the gametophytes contain ϰ- and two additional carrageenans which are kcl-soluble and may comprise up to 25% of the total carrageenan. after alkaline modification, these kcl-soluble components were separated into a gel and a soluble carrageenan. the gel was indistinguishable from ϰ-carrageenan and presumably was derived from μ-carrag ...197324468729
purification and characterization of hexose oxidase from the red alga chondrus crispus. 19734708670
plasmalemmal extensions in chondrus crispus (l.) stackh. 19714329670
the incorporation of s-labeled sulfate into carrageenan in chondrus crispus. 197116657801
heterogeneity of carrageenans from chondrus crispus.carrageenans extracted from gametophytic and sporophytic chondrus crispus were analysed by hydrolysis, kcl fractionation and 1h nmr spectroscopy. the carrageenan from gametophytic plants is composed predominantly of two kcl insoluble fractions which contain kappa-carrageenan as the major component with 1-carrageenan and sulphated galactans as minor components. the precursor mu- and v-carrageenans were not found in the soluble fraction. the extract from sporophytic plants is composed mainly of a ...19701367970
sterols in irish moss (chondrus crispus). 19665968606
amino acids, peptides, and proteins of irish moss, chondrus crispus. 195813563510
effect of processing and storage on the quality of gelose from irish moss (chondrus crispus). 194818916788
2-keto-d-gluconic acid in the polysaccharide of irish moss. 194620989466
2-keto-d-gluconic acid in the polysaccharide of irish moss. 194621064272
a method of measuring the stabilizing power of extracts of irish moss. 194621015724
the effect of solvent and temperature on the viscosity of the polysaccharide or irish moss and the effect of solvent on its initial gelation. 194621015723
seasonal variations in chondrus crispus. 193616746163
a note on the nitrogen of the polysaccharide complex from chondrus crispus. 193516745757
some properties of the polysaccharide complex extracted from a marine alga, chondrus crispus. 193416745459
on carrageen (chondrus crispus): the hydrolysis of carrageen mucilage. 192916744227
on carrageen (chondrus crispus). iii: the constitution of the cell wall. 192216743120
on carrageen (chondrus crispus). ii: on the occurrence of ethereal sulphates in the plant. 192116743011
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