Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| rapid and profound shifts in the vaginal microbiota following antibiotic treatment for bacterial vaginosis. | bacterial vaginosis (bv) is a common polymicrobial disease associated with numerous negative reproductive health outcomes, including an increased risk of human immunodeficiency virus acquisition. bv is treatable with antibiotics, but relapse is common. a more detailed understanding of bacterial dynamics during antibiotic therapy for bv could identify conditions that favor establishment, maintenance, and eradication of bv-associated bacterial species, thereby improving treatment outcomes. | 2015 | 25676470 |
| draft genome sequence of a metronidazole-resistant gardnerella vaginalis isolate. | we report the draft genome sequence of a gardnerella vaginalis strain (3549624) isolated from a vaginal specimen. g. vaginalis is associated with bacterial vaginosis, the most common cause of vaginal discharge, which is often treated with metronidazole. this isolate is highly resistant to metronidazole (mic, 500 µg/ml) and may be useful for comparative genomic studies to determine the molecular basis of metronidazole resistance in this species. | 2015 | 26337887 |
| new assay for gardnerella vaginalis loads correlates with nugent scores and has potential in the diagnosis of bacterial vaginosis. | gardnerella vaginalis is a gram-variable anaerobic bacterium present in 100% of women with bacterial vaginosis (bv). bv is a complex polymicrobial condition with no single causative agent. the current laboratory detection method for bv relies on a gram-stain nugent score to estimate the quantity of different bacterial morphotypes in the vaginal micro flora. whilst the nugent score can distinguish between women with and without bv, a significant proportion are categorized as intermediate, which f ... | 2015 | 26296660 |
| changes in vaginal microbiota following antimicrobial and probiotic therapy. | the composition of the vaginal microbiota is known to be important for health. when infections occur, antimicrobial therapy is often poorly efficacious. | 2015 | 26282697 |
| using an in-vitro biofilm model to assess the virulence potential of bacterial vaginosis or non-bacterial vaginosis gardnerella vaginalis isolates. | gardnerella vaginalis is the most common species found in bacterial vaginosis (bv). however, it is also present in a significant proportion of healthy women and g. vaginalis vaginal colonization does not always lead to bv. in an effort to better understand the differences between g. vaginalis isolated from women with a positive (bv) versus a negative (non-bv) diagnosis of bv, we compared the virulence potential of 7 bv and 7 non-bv g. vaginalis isolates and assessed the virulence factors related ... | 2015 | 26113465 |
| the cytolytic activity of vaginolysin strictly depends on cholesterol and is potentiated by human cd59. | gardnerella vaginalis produces cytolysin vaginolysin (vly), which has been suggested to be a contributor to bacterial vaginosis pathogenesis. vly along with intermedilysin (ily) from streptococcus intermedius have been attributed to a group of cholesterol-dependent cytolysins (cdcs) whose pore-forming activity depends on human cd59 (hcd59). here, we show that different types of cells lacking hcd59 are susceptible to vly-mediated lysis, albeit to different extents. we analyze the effects of both ... | 2015 | 25590277 |
| the natural antimicrobial subtilosin a synergizes with lauramide arginine ethyl ester (lae), ε-poly-l-lysine (polylysine), clindamycin phosphate and metronidazole, against the vaginal pathogen gardnerella vaginalis. | bacterial vaginosis (bv) is a common, recurrent vaginal infection linked to increased chances of preterm delivery, incidence of sexually transmitted infections and fertility problems. bv is caused by a shift of the vaginal ecosystem from predominately lactobacillus to a multispecies actinomyces biofilm with the most common representatives identified as gardnerella vaginalis and prevotella spp. current treatments have been associated with increased resistance as well as negative effects on health ... | 2015 | 25588687 |
| ethnic variations in cervical cytokine concentrations and vaginal flora during pregnancy. | pregnancy-mediated changes in immunity may influence risk of hiv-1 acquisition. this risk appears greatest among non-caucasian women. | 2015 | 25041358 |
| gardnerella vaginalis does not always cause bacterial vaginosis. | 2014 | 24855684 | |
| performance of the affirm vp-iii using residual vaginal discharge collected from the speculum to characterize vaginitis in symptomatic women. | to evaluate the ability of collecting the affirm vp-iii test sample using the residual vaginal discharge found on the speculum. | 2014 | 24832170 |
| rifaximin modulates the vaginal microbiome and metabolome in women affected by bacterial vaginosis. | bacterial vaginosis (bv) is a common vaginal disorder characterized by the decrease of lactobacilli and overgrowth of gardnerella vaginalis and resident anaerobic vaginal bacteria. in the present work, the effects of rifaximin vaginal tablets on vaginal microbiota and metabolome of women affected by bv were investigated by combining quantitative pcr and a metabolomic approach based on (1)h nuclear magnetic resonance. to highlight the general trends of the bacterial communities and metabolomic pr ... | 2014 | 24709255 |
| evaluation of polycaprolactone matrices for the intravaginal delivery of metronidazole in the treatment of bacterial vaginosis. | microporous, poly (ɛ-caprolactone) (pcl) matrices loaded with the antibacterial, metronidazole were produced by rapidly cooling suspensions of drug powder in pcl solutions in acetone. drug incorporation in the matrices increased from 2.0% to 10.6% w/w on raising the drug loading of the pcl solution from 5% to 20% w/w measured with respect to the pcl content. drug loading efficiencies of 40-53% were obtained. rapid 'burst release' of 35-55% of the metronidazole content was recorded over 24 h when ... | 2014 | 24682036 |
| trichomonas, candida, and gardnerella in cervical smears of iranian women for cancer screening. | trichomonas vaginalis, gardrenella vaginalis, and candida sp are responsible for 90% of vaginitis which has been reported as important risk factors for cervical cancer. | 2014 | 24678473 |
| seasonal variation of antibacterial activities in the green alga ulva pertusa kjellman. | the present study was performed to screen out the extracts of algae and assess the seasonal variation in antimicrobial activity of ulva pertusa against gardnerella vaginalis. seasonal variation in antibacterial activity was observed, with the extracts showing no activity during summer and autumn, and showing antibacterial activity from early winter (december) to middle spring (april). the maximum value of antimicrobial activity (6.5 mm inhibition zone at 5 mg disk(-1)) of u. pertusa against g. v ... | 2014 | 24665759 |
| curative effect of the probiotic strain lactobacillus fermentum l23 in a murine model of vaginal infection by gardnerella vaginalis. | bacterial vaginosis is a common vaginal infection characterized by changes in the vaginal microbiota. the objective of this work was to evaluate the colonization ability and curative effect of lactobacillus fermentum l23 after vaginal administration in female balb/c mice infected with gardnerella vaginalis. one dose of lact. fermentum l23 containing 10(9 ) cfu ml(-1) was administered locally in a murine vaginal model. l23 colonized the vaginal tract of balb-c mice after one inoculation. the infe ... | 2014 | 24641524 |
| gardnerella vaginalis outcompetes 29 other bacterial species isolated from patients with bacterial vaginosis, using in an in vitro biofilm formation model. | despite the worldwide prevalence of bacterial vaginosis (bv), its etiology is still unknown. although bv has been associated with the presence of biofilm, the ability of bv-associated bacteria to form biofilms is still largely unknown. here, we isolated 30 bv-associated species and characterized their virulence, using an in vitro biofilm formation model. our data suggests that gardnerella vaginalis had the highest virulence potential, as defined by higher initial adhesion and cytotoxicity of epi ... | 2014 | 24596283 |
| eswab as an optional collection device for use with the affirm vpiii microbial test system. | the eswab collection device was compared to the collection swab provided as part of the affirm vpiii microbial identification test kit for testing vaginal specimens with the affirm test system. there was excellent agreement between the two sampling devices for candida spp., gardnerella vaginalis, and trichomonas vaginalis. | 2014 | 24523475 |
| role of gardnerella vaginalis in the pathogenesis of bacterial vaginosis: a conceptual model. | bacterial vaginosis (bv) is the most common cause of vaginal discharge and is associated with important public health complications such as preterm birth and acquisition or transmission of human immunodeficiency virus and sexually transmitted infections. continued controversy concerning the pathogenesis of bv has led to a lack of progress in prevention and management of this infection. | 2014 | 24511102 |
| the diversity of receptor recognition in cholesterol-dependent cytolysins. | cholesterol-dependent cytolysins (cdcs) are bacterial pore-forming toxins secreted mainly by pathogenic gram-positive bacteria. cdcs generally recognize and bind to membrane cholesterol to create pores and lyse target cells. however, in contrast to typical cdcs such as streptolysin o, several atypical cdcs have been reported. the first of these was intermedilysin, which is secreted by streptococcus intermedius and has human cell-specificity, human cd59 (hucd59) being its receptor. in the study r ... | 2014 | 24401114 |
| [risk factors for bacterial vaginosis - a 3-year study in four medical centers]. | bacterial vaginosis (bv) is the most common infection of the lower genital tract among women of reproductive age. the purpose of this study was to determine the frequency of bv and the important etiological agent gardnerella vaginalis in bulgarian patients of different age groups, as well as, the risk factors forbv methods: one thousand five hundred and twenty-three (1523) women aged 16 to 45 years without previous antimicrobial therapy were included in this study. the methods used were: scoring ... | 2014 | 25509639 |
| comparative genomics of lactobacillus crispatus suggests novel mechanisms for the competitive exclusion of gardnerella vaginalis. | lactobacillus crispatus is a ubiquitous micro-organism encountered in a wide range of host-associated habitats. it can be recovered from the gastrointestinal tract of animals and it is a common constituent of the vaginal microbiota of humans. moreover, l. crispatus can contribute to the urogenital health of the host through competitive exclusion and the production of antimicrobial agents. in order to investigate the genetic diversity of this important urogenital species, we performed a comparati ... | 2014 | 25480015 |
| breast cancer, aromatase inhibitor therapy, and sexual functioning: a pilot study of the effects of vaginal testosterone therapy. | women with breast cancer have better cancer-related outcomes with the use of aromatase inhibitors (ais), but the physiological suppression of estradiol can negatively affect sexual functioning because of unpleasant urogenital and vaginal symptoms. local health care practitioners have observed that the benefits of vaginal testosterone in allaying these unpleasant symptoms in women with breast cancer are similar to the benefits of vaginal estrogen in women without breast cancer. | 2014 | 25356296 |
| recent progress in understanding the epidemiology of bacterial vaginosis. | bacterial vaginosis epidemiology has been transformed by new theoretical insights and methodologies, such as molecular sequencing. we summarize the progress made in these domains. | 2014 | 25304606 |
| gardnerella vaginalis triggers nlrp3 inflammasome recruitment in thp-1 monocytes. | gardnerella vaginalis is a gram-positive bacterium associated with bacterial vaginosis (bv), pelvic inflammatory disease, and preterm birth. bv is the most prevalent vaginal dysbiosis in women of childbearing age characterized by the absence of normal lactobacilli and an overgrowth of g. vaginalis and other bacteria. although mucosal fluids from bv patients exhibit increases in proinflammatory cytokines and toll-like receptor 2 and 4 mrna, g. vaginalis has not been demonstrated to directly induc ... | 2014 | 25280956 |
| prevalence of the genital tract bacterial infections after vaginal reconstructive surgery. | due to frequent childbirth, heavy lifting and the structure of the lives of rural women in shahrekord region, iran, cystocele and rectocele are of the main medical problems of the women in this area and for its correction, vaginal reconstructive surgery is needed which causes infection. the purpose of this study was to identify the bacteria causing infection after vaginal reconstructive surgery and performing antibiogram to help these patients for faster recovery. patients enrolled this study we ... | 2014 | 26031026 |
| [condition of periodontal tissue in women with bacterial vaginosis]. | cross colonization of open cavities of human body has little been studied in contemporary medicine. there in no practical recommendations as for management of dental patients with urogenital tract microflora disorder. the paper presents the findings of clinical features of periodontium tissues in women with bacterial vaginosis correlated with bacteriological estimation of oral cavity and vagina, number of sexual partners. interpretation of amine test of oral and vaginal fluids has been provided ... | 2014 | 25617096 |
| characterization of the vaginal microbiota of healthy canadian women through the menstrual cycle. | the vaginal microbial community plays a vital role in maintaining women's health. understanding the precise bacterial composition is challenging because of the diverse and difficult-to-culture nature of many bacterial constituents, necessitating culture-independent methodology. during a natural menstrual cycle, physiological changes could have an impact on bacterial growth, colonization, and community structure. the objective of this study was to assess the stability of the vaginal microbiome of ... | 2014 | 25053998 |
| term and preterm labour are associated with distinct microbial community structures in placental membranes which are independent of mode of delivery. | infection is considered a possible trigger for preterm labour, supported by evidence showing the presence of bacteria in the placenta and placental membranes from preterm births. in this study, 16s rdna pyrosequencing was used to identify bacteria in placental membranes. caesarean sections and vaginal deliveries at term were found to harbour common genera. mycoplasma hominis, aerococcus christensenii, gardnerella vaginalis and fusobacterium nucleatum were either only present in preterm membranes ... | 2014 | 25458966 |
| effectiveness of the two microorganisms lactobacillus fermentum lf15 and lactobacillus plantarum lp01, formulated in slow-release vaginal tablets, in women affected by bacterial vaginosis: a pilot study. | bacterial vaginosis (bv) is the most common reason for abnormal vaginal discharge in reproductive-age women and one of its most important causative agents is the gram-variable bacterium gardnerella vaginalis. bv is not accompanied by significant local inflammation, whereas the "fishy odor" test is always positive. in contrast, aerobic vaginitis (av) is predominantly associated with escherichia coli, but streptococcus agalactiae and staphylococcus aureus are also involved. standard treatment of b ... | 2014 | 25291116 |
| lactobacillus crispatus dominant vaginal microbiome is associated with inhibitory activity of female genital tract secretions against escherichia coli. | female genital tract secretions inhibit e. coli ex vivo and the activity may prevent colonization and provide a biomarker of a healthy microbiome. we hypothesized that high e. coli inhibitory activity would be associated with a lactobacillus crispatus and/or jensenii dominant microbiome and differ from that of women with low inhibitory activity. | 2014 | 24805362 |
| epidemiology of sexually transmitted infections in rural southwestern haiti: the grand'anse women's health study. | the study attempts to define socioeconomic, clinical, and laboratory correlates in vaginitis and other sexually transmitted infections in rural southwestern haiti. a convenience sample of subjects recruited from a rural women's health clinic and attending an established clinic at the haitian health foundation (hhf) clinic was studied. a standardized history and physical examination, including speculum examination, and collection of blood, urine, and vaginal swabs were obtained from the women at ... | 2014 | 25200263 |
| inhibitory effects of seaweed extracts on the growth of the vaginal bacterium gardnerella vaginalis. | of 44 species of seaweed screened for potential anti-gardnerella vaginalis activity, 27 (61.4%) showed antimicrobial activity by the agar disk-diffusion method. among them, the strongest activities against the pathogen were exhibited by chlorophyta, with ulva pertusa producing an 11.3-mm zone of inhibition at 5 mg disk⁻¹. the mic values of u. pertusa extracts against both g. vaginalis kctc 5096 and kctc 5097, the main cause of vaginosis, were 312 μg ml⁻¹, while the mic values against both candid ... | 2014 | 24813010 |
| [place of vulvovaginal candidiasis in the lower genital tract infections and associated risk factors among women in benin]. | determine the place of vulvo-vaginal candidiasis (vvc) in the lower genital infections and seek risk factors among women in benin. | 2014 | 24746421 |
| identification of vaginal lactobacilli with potential probiotic properties isolated from women in north lebanon. | the aim of this work was to study the diversity of vaginal lactobacilli in lebanese women and to evaluate the antagonism, hydrophobicity, and safety characteristics of these strains. this study was performed on samples from 135 women who visited a gynecology clinic in the north of lebanon, between september 2012 and january 2013. from these samples, 53 different isolates of vaginal lactobacilli were collected from vaginal swabs and identified using biochemical and molecular methods. the use of g ... | 2014 | 24549747 |
| tissue-specific il-10 secretion profile from term human fetal membranes stimulated with pathogenic microorganisms associated with preterm labor in a two-compartment tissue culture system. | interleukin (il)-10 is a cytokine with anti-inflammatory properties that plays pivotal roles in immune recognition and maintenance of pregnancy, limiting the harmful effects of pro-inflammatory modulators. the aim of this work was to characterize the contribution of amnion and choriodecidua regions of the human fetal membranes in the production of il-10 after selective stimulation with candida albicans, gardnerella vaginalis and streptococcus agalactiae. | 2014 | 24138141 |
| characterization of the vaginal microflora in health and disease. | bacterial vaginosis (bv) is an imbalance of the vaginal bacterial microbiota and its aetiology is still unknown. our aims were to investigate the diagnostic potential of species/genus specific quantitative pcr (qpcr) for bacteria present in swabs and first-void urine (fvu) samples using nugent's and claeys' criteria and 454 sequencing of the vaginal microbiome as reference. | 2014 | 24814599 |
| bead-based multiplex sexually transmitted infection profiling. | sexually transmitted infections are a significant cause of genital disease, infertility and hospital admissions. the economic impact is high. an accurate diagnosis is often difficult and time consuming. we report the development and validation of a novel bead-based multiplex sexually transmitted infection profiling (stip) assay that detects 18 sexually transmitted infections using a multiplex pcr followed by luminex bead-based hybridisation. | 2014 | 24814157 |
| prevalence of gardnerella vaginalis and atopobium vaginae in portuguese women and association with risk factors for bacterial vaginosis. | 2014 | 24268352 | |
| role of gardnerella vaginalis as an etiological agent of bacterial vaginosis. | bacterial vaginosis is a risk factor for obstetric infections, various adverse outcomes of pregnancy and pelvic inflammatory disease. the objectives of this study were to assess the prevalence of bacterial vaginosis in women attending gynaecology outpatient department (o.p.d) and sexually transmitted disease (s.t.d.) clinic and to assess the role of gardnerella vaginalis as an etiological agent of bacterial vaginosis. | 2014 | 25926959 |
| polyethylene glycol-based hydrogels for controlled release of the antimicrobial subtilosin for prophylaxis of bacterial vaginosis. | current treatment options for bacterial vaginosis (bv) have been shown to be inadequate at preventing recurrence and do not provide protection against associated infections, such as that with hiv. this study examines the feasibility of incorporating the antimicrobial peptide subtilosin within covalently cross-linked polyethylene glycol (peg)-based hydrogels for vaginal administration. the peg-based hydrogels (4% and 6% [wt/vol]) provided a two-phase release of subtilosin, with an initial rapid r ... | 2014 | 24566190 |
| bacterial vaginosis diagnosed by analysis of first-void-urine specimens. | bacterial vaginosis (bv) is traditionally diagnosed using vaginal samples. the aim of this study was to investigate whether bv can be diagnosed from first-void urine (fvu). self-collected vaginal smears, vaginal swabs, and fvu were obtained from 176 women. bv was diagnosed by nugent's criteria. the fvu and vaginal swabs were analyzed by quantitative pcrs (qpcrs) for selected vaginal bacteria (atopobium vaginae, prevotella spp., gardnerella vaginalis, bacterial vaginosis-associated bacterium 2, e ... | 2014 | 24197876 |
| quantification of atopobium vaginae loads may be a new method for the diagnosis of bacterial vaginosis. | traditional methods for the diagnosis of bv is either with poor sensitivity or poor specificity. thus, establishing a new method based on the vaginal flora is vital for the diagnosis of bv. | 2014 | 25291946 |
| co-infection of human immunodeficiency virus and sexually transmitted infections in circumcised and uncircumcised cases in india. | acquired immunodeficiency syndrome (aids), is now one of the greatest challenges facing the world. sexual transmission is the primary route of human immunodeficiency virus infection worldwide. male circumcision is being considered as strategy to reduce the burden of hiv/aids. | 2014 | 26396445 |
| poly(ethylene glycol) (peg)-lactic acid nanocarrier-based degradable hydrogels for restoring the vaginal microenvironment. | women with bacterial vaginosis (bv) display reduced vaginal acidity, which make them susceptible to associated infections such as hiv. in the current study, poly(ethylene glycol) (peg) nanocarrier-based degradable hydrogels were developed for the controlled release of lactic acid in the vagina of bv-infected women. peg-lactic acid (peg-la) nanocarriers were prepared by covalently attaching lactic acid to 8-arm peg-sh via cleavable thioester bonds. peg-la nanocarriers with 4 copies of lactic acid ... | 2014 | 25223229 |
| human-specific bacterial pore-forming toxins induce programmed necrosis in erythrocytes. | a subgroup of the cholesterol-dependent cytolysin (cdc) family of pore-forming toxins (pfts) has an unusually narrow host range due to a requirement for binding to human cd59 (hcd59), a glycosylphosphatidylinositol (gpi)-linked complement regulatory molecule. hcd59-specific cdcs are produced by several organisms that inhabit human mucosal surfaces and can act as pathogens, including gardnerella vaginalis and streptococcus intermedius. the consequences and potential selective advantages of such p ... | 2014 | 25161188 |
| differences in vaginal microbiome in african american women versus women of european ancestry. | women of european ancestry are more likely to harbour a lactobacillus-dominated microbiome, whereas african american women are more likely to exhibit a diverse microbial profile. african american women are also twice as likely to be diagnosed with bacterial vaginosis and are twice as likely to experience preterm birth. the objective of this study was to further characterize and contrast the vaginal microbial profiles in african american versus european ancestry women. through the vaginal human m ... | 2014 | 25073854 |
| increased gardnerella vaginalis urogenital biofilm in inflammatory bowel disease. | inflammatory bowel disease (ibd) is a systemic inflammatory condition that affects the entire organism, not only the bowel. an impaired interaction with microbiota has been shown to be important. we looked for bacterial factors, which may contribute to the well-known higher incidence of poor reproductive outcome in ibd. | 2014 | 24315776 |
| prevalence of gardnerella vaginalis among women with lactobacillus-predominant vaginal flora. | to determine the prevalence of gardnerella vaginalis in women with normal vaginal flora. | 2014 | 24259188 |
| identification, quantification and subtyping of gardnerella vaginalis in noncultured clinical vaginal samples by quantitative pcr. | gardnerella vaginalis is an important component of the human vaginal microflora. it is proposed to play a key role in the pathogenesis of bacterial vaginosis (bv), the most common vaginal condition. here we describe the development, validation and comparative analysis of a novel molecular approach capable of g. vaginalis identification, quantification and subtyping in noncultured vaginal specimens. using two quantitative pcr (qpcr) assays, we analysed g. vaginalis bacterial loads and clade distr ... | 2014 | 24200640 |
| human epididymis protein 4 and secretory leukocyte protease inhibitor in vaginal fluid: relation to vaginal components and bacterial composition. | human epididymis protein 4 (he4) is a protease inhibitor and a recently identified serum biomarker for ovarian cancer. properties of he4 in the genital tract of healthy women have not been evaluated. we evaluated associations between he4 and a second vaginal protease inhibitor, secretory leukocyte protease inhibitor (slpi), with vaginal concentrations of innate immune mediators or proteases and with the types of vaginal bacterial communities. vaginal secretions were collected from 18 healthy rep ... | 2014 | 24023032 |
| [gardnerella vaginalis bacteremia in a male patient]. | 2013 | 24016769 | |
| microbial and vaginal determinants influencing mycoplasma hominis and ureaplasma urealyticum genital colonization in a population of female patients. | mycoplasma hominis and ureaplasma urealyticum are associated with chorioamnionitis, preterm delivery and pelvic inflammatory disease. the aim of this study was to evaluate the possible risk factors of co-colonization by m. hominis in patients already colonized by u. urealyticum and compare demographic parameters, vaginal ph and microbiota of women colonized by u. urealyticum or m. hominis. a total of 452 patients positive for u. urealyticum or m. hominis were analysed, 421 (93.1%) of whom were p ... | 2013 | 24008852 |
| relationship of selected bacterial vaginosis-associated bacteria to nugent score bacterial vaginosis among urban women early in pregnancy. | among urban, primarily african american pregnant women, 74% were identified with nugent score bacterial vaginosis (bv). all bv-associated bacteria were more prevalent among women with nugent score bv. bacterial vaginosis-associated bacteria 3 (bvab3) had the highest positive predictive value, whereas gardnerella vaginalis and atopobium spp. had the highest sensitivity. atopobium spp. levels had the most significant area under the curve. | 2013 | 23945426 |
| reciprocal interference between lactobacillus spp. and gardnerella vaginalis on initial adherence to epithelial cells. | bacterial vaginosis (bv) is the most common vaginal disorder in women of child-bearing age. it is widely accepted that the microbial switch from normal microflora to the flora commonly associated with bv is characterized by a decrease in vaginal colonization by specific lactobacillus species together with an increase of g. vaginalis and other anaerobes. however, the order of events leading to the development of bv remains poorly characterized and it is unclear whether the decrease in lactobacill ... | 2013 | 23935396 |
| quantitative analysis of initial adhesion of bacterial vaginosis-associated anaerobes to me-180 cells. | bacterial vaginosis is the leading vaginal disorder but the transition from health to this dysbiotic condition remains poorly characterized. our goal was to quantify the ability of bv-associated anaerobes to adhere to epithelial cells in the presence of lactobacilli. gardnerella vaginalis outcompeted lactobacillus crispatus and lactobacillus iners actually enhanced its adherence. | 2013 | 23916636 |
| [diagnosis of vaginitis-vaginosis by hibridization with dna strands]. | vaginal infections lie among the most common causes women ask for medical advice. in order of frequency bacterial vaginosis, vulvovaginal candidiasis, and trichomoniasis are responsible for 90% of vaginitis/vaginosis. | 2013 | 23720932 |
| the biofilm in bacterial vaginosis: implications for epidemiology, diagnosis and treatment. | recent evidence supports the view that bacterial vaginosis presents as a polymicrobial biofilm infection. this has far-reaching implications for the pathogenesis, epidemiology, diagnosis and treatment of bacterial vaginosis. | 2013 | 23221767 |
| vaginolysin drives epithelial ultrastructural responses to gardnerella vaginalis. | gardnerella vaginalis, the bacterial species most frequently isolated from women with bacterial vaginosis (bv), produces a cholesterol-dependent cytolysin (cdc), vaginolysin (vly). at sublytic concentrations, cdcs may initiate complex signaling cascades crucial to target cell survival. using live-cell imaging, we observed the rapid formation of large membrane blebs in human vaginal and cervical epithelial cells (vk2 and hela cells) exposed to recombinant vly toxin and to cell-free supernatants f ... | 2013 | 24082080 |
| are plant extracts a potential therapeutic approach for genital infections? | more than 40% of the new drugs registered from 1981 to 2006 were obtained, derived or inspired from natural compounds. the influence of natural products in the anti-infective area is quite marked, being a great percentage of drugs derived or extracted from natural products. vaginal infections are one of the most common reasons a women visits a gynecologist. given the high popularity of natural therapies among women who suffer from chronic infections, it is urgent for women's healthcare providers ... | 2013 | 23651308 |
| gardnerella vaginalis-associated bacterial vaginosis in bulgarian women. | bacterial vaginosis (bv) is the most common cause of vaginal discharge in women of reproductive age. the purpose of this study was to determine the frequency of bv in bulgarian pregnant and nonpregnant women from several age ranges and to compare three different laboratory methods for gardnerella vaginalis detection in patents suffering from bv. | 2013 | 23602465 |
| fluorescence in situ hybridization method using peptide nucleic acid probes for rapid detection of lactobacillus and gardnerella spp. | bacterial vaginosis (bv) is a common vaginal infection occurring in women of reproductive age. it is widely accepted that the microbial switch from normal microflora to bv is characterized by a decrease in vaginal colonization by lactobacillus species together with an increase of gardnerella vaginalis and other anaerobes. our goal was to develop and optimize a novel peptide nucleic acid (pna) fluorescence in situ hybridization assay (pna fish) for the detection of lactobacillus spp. and g. vagin ... | 2013 | 23586331 |
| clinical features of bacterial vaginosis in a murine model of vaginal infection with gardnerella vaginalis. | bacterial vaginosis (bv) is a dysbiosis of the vaginal flora characterized by a shift from a lactobacillus-dominant environment to a polymicrobial mixture including actinobacteria and gram-negative bacilli. bv is a common vaginal condition in women and is associated with increased risk of sexually transmitted infection and adverse pregnancy outcomes such as preterm birth. gardnerella vaginalis is one of the most frequently isolated bacterial species in bv. however, there has been much debate in ... | 2013 | 23527214 |
| degradation, foraging, and depletion of mucus sialoglycans by the vagina-adapted actinobacterium gardnerella vaginalis. | bacterial vaginosis (bv) is a polymicrobial imbalance of the vaginal microbiota associated with reproductive infections, preterm birth, and other adverse health outcomes. sialidase activity in vaginal fluids is diagnostic of bv and sialic acid-rich components of mucus have protective and immunological roles. however, whereas mucus degradation is believed to be important in the etiology and complications associated with bv, the role(s) of sialidases and the participation of individual bacterial s ... | 2013 | 23479734 |
| dnase inhibits gardnerella vaginalis biofilms in vitro and in vivo. | bacterial vaginosis is a highly prevalent and poorly understood polymicrobial disorder of the vaginal microbiota, with significant adverse sequelae. gardnerella vaginalis predominates in bacterial vaginosis. biofilms of g. vaginalis are present in human infections and are implicated in persistent disease, treatment failure, and transmission. here we demonstrate that g. vaginalis biofilms contain extracellular dna, which is essential to their structural integrity. enzymatic disruption of this dna ... | 2013 | 23431033 |
| gardnerella vaginalis: still a prime suspect in the pathogenesis of bacterial vaginosis. | bacterial vaginosis (bv) is the most common cause of vaginal infection, yet its pathogenesis remains controversial. although it has never been proven to be a sexually transmitted diseases the epidemiological evidence favoring this is quite robust. although bv is characterized by its polymicrobial nature, it is highly likely that the inciting organism is gardnerella vaginalis. | 2013 | 23371405 |
| presence of a polymicrobial endometrial biofilm in patients with bacterial vaginosis. | to assess whether the bacterial vaginosis biofilm extends into the upper female genital tract. | 2013 | 23320114 |
| human papillomavirus and other genital infections in indigenous women from paraguay: a cross-sectional analytical study. | the incidence of cervical cancer in paraguay is among the highest in the world, with the human papillomavirus (hpv) being a necessary factor for cervical cancer. knowledge about hpv infection among indigenous women is limited. this cross-sectional study analyzed the frequency of hpv and other genital infections in indigenous paraguayan women of the department of presidente hayes. | 2013 | 24206645 |
| vaginal microbiome in women from greenland assessed by microscopy and quantitative pcr. | bacterial vaginosis (bv) is a common condition, although its aetiology remains unexplained. the aim of this study was to analyse the composition of vaginal microbiota in women from greenland to provide a quantitative description and improve the understanding of bv. | 2013 | 24131550 |
| biomedical applications of fermenticin hv6b isolated from lactobacillus fermentum hv6b mtcc10770. | fermenticin hv6b is a class iia antimicrobial peptide produced by lactobacillus fermentum hv6b mtcc 10770 isolated from human vaginal ecosystem. it shows growth inhibition of a wide range of opportunistic pathogens of humans, for example, bacteroides, gardnerella vaginalis, mobiluncus, staphylococci, and streptococci, associated with bacterial vaginosis in humans. it does possess an impressive sperm immobilization and spermicidal activity tested against human sperms which makes it an attractive ... | 2013 | 23984320 |
| enterococcus faecalis inhibits superantigen toxic shock syndrome toxin-1-induced interleukin-8 from human vaginal epithelial cells through tetramic acids. | the vaginal mucosa can be colonized by many bacteria including commensal organisms and potential pathogens, such as staphylococcus aureus. some strains of s. aureus produce the superantigen toxic shock syndrome toxin-1, which can penetrate the vaginal epithelium to cause toxic shock syndrome. we have observed that a female was mono-colonized with enterococcus faecalis vaginally as tested in aerobic culture, even upon repeated culture for six months, suggesting this organism was negatively influe ... | 2013 | 23613823 |
| composition of the vaginal microbiota in women of reproductive age--sensitive and specific molecular diagnosis of bacterial vaginosis is possible? | bacterial vaginosis (bv) is the most common vaginal disorder, characterized by depletion of the normal lactobacillus-dominant microbiota and overgrowth of commensal anaerobic bacteria. this study aimed to investigate the composition of the vaginal microbiota in women of reproductive age (healthy women and women with bv), with the view of developing molecular criteria for bv diagnosis. | 2013 | 23585843 |
| comparison of the vaginal microbiota diversity of women with and without human papillomavirus infection: a cross-sectional study. | the female genital tract is an important bacterial habitat of the human body, and vaginal microbiota plays a crucial role in vaginal health. the alteration of vaginal microbiota affects millions of women annually, and is associated with numerous adverse health outcomes, including human papillomavirus (hpv) infection. however, previous studies have primarily focused on the association between bacterial vaginosis and hpv infection. little is known about the composition of vaginal microbial communi ... | 2013 | 23758857 |
| [diagnosis of bacterial vaginosis]. | bacterial vaginosis is a common, complex clinical syndrome characterized by alterations in the normal vaginal flora. when symptomatic, it is associated with a malodorous vaginal discharge and on occasion vaginal burning or itching. under normal conditions, lactobacilli constitute 95% of the bacteria in the vagina. bacterial vaginosis is associated with severe reduction or absence of the normal h2o2-producing lactobacilli and overgrowth of anaerobic bacteria and gardnerella vaginalis, atopobium v ... | 2013 | 24073569 |
| the villain team-up or how trichomonas vaginalis and bacterial vaginosis alter innate immunity in concert. | complex interactions of vaginal microorganisms with the genital tract epithelium shape mucosal innate immunity, which holds the key to sexual and reproductive health. bacterial vaginosis (bv), a microbiome-disturbance syndrome prevalent in reproductive-age women, occurs commonly in concert with trichomoniasis, and both are associated with increased risk of adverse reproductive outcomes and viral infections, largely attributable to inflammation. to investigate the causative relationships among in ... | 2013 | 23903808 |
| plants traditionally used individually and in combination to treat sexually transmitted infections in northern maputaland, south africa: antimicrobial activity and cytotoxicity. | although medicinal plants are used extensively to treat sexually transmitted infections (stis) in rural northern maputaland, kwazulu-natal, the efficacy and safety of these plants have not previously been evaluated. | 2013 | 23880127 |
| [sexually transmitted infections in women living in a prison in lima, peru]. | in order to determine the prevalence of sexually transmitted infections (sti) in recluse women of chorrillos penitentiary i in the city of lima, peru, a cross-sectional study was performed, including a simple random sample of women between ages 18 and 54, from whom 180 serum samples and 168 samples of cervical and vaginal discharge were analyzed. the most prevalent etiologic agents identified from cervical and vaginal discharge samples were chlamydia trachomatis in 42.3%, gardnerella vaginalis i ... | 2013 | 24100816 |
| bacterial infections of the lower genital tract in fertile and infertile women from the southeastern poland. | the objective of the study was to investigate the detection rates of chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum, gardnerella vaginalis, escherichia coli, streptococcus agalactiae and enterococcus faecalis, showing no clinical signs of an ongoing, acute inflammatory state of the vagina and/or the cenrvix, in fertile and infertile women. | 2013 | 23819400 |
| [bacterial infection as a cause of infertility in humans]. | microorganisms which are present in the human urogenital tract may be involved in the development of inflammatory changes negatively affecting the genitals in both men and women. pathological conditions due to inflammatory alterations may result in complete loss of fertility. infections of the urogenital tract are responsible for 15% of all cases of infertility in couples. negative impact on the human reproduction is mainly caused by direct damage to the genital tract mucosa by metabolic product ... | 2013 | 23768092 |
| sequences of multiple bacterial genomes and a chlamydia trachomatis genotype from direct sequencing of dna derived from a vaginal swab diagnostic specimen. | ultra-deep illumina sequencing was performed on whole genome amplified dna derived from a chlamydia trachomatis-positive vaginal swab. alignment of reads with reference genomes allowed robust snp identification from the c. trachomatis chromosome and plasmid. this revealed that the c. trachomatis in the specimen was very closely related to the sequenced urogenital, serovar f, clade t1 isolate f-sw4. in addition, high genome-wide coverage was obtained for prevotella melaninogenica, gardnerella vag ... | 2013 | 23647919 |
| simultaneous direct identification of genital microorganisms in voided urine using multiplex pcr-based reverse line blot assays. | our aim was to develop and evaluate sensitive methods that would allow simultaneous direct identification of multiple potential pathogens in clinical specimens for diagnosis and epidemiological studies, using a multiplex pcr-based reverse line blot assay. we have previously developed assays suitable for detection of bacterial respiratory and systemic pathogens. in this chapter we describe, in detail, a method developed to identify 14 genital microorganisms, for use in epidemiological studies of ... | 2013 | 23104293 |
| in vitro probiotic properties of lactobacillus fermentum sk5 isolated from vagina of a healthy woman. | a lactobacillus strain isolated from a vaginal tract of a healthy woman was examined in vitro for its probiotic potential. this strain, identified as lactobacillus fermentum sk5, was able to survive at ph 3-4 and 0.1-0.2% bile, and unaffected by pepsin (3 g l(-1)) and pancreatin (1 g l(-1)), but was susceptible to all tested antibiotics except metronidazole. l. fermentum sk5 had an antimicrobial potential against gastrointestinal pathogenic escherichia coli and vaginal pathogenic gardnerella vag ... | 2013 | 23624069 |
| pregnancy-induced changes in immune protection of the genital tract: defining normal. | both the state of pregnancy as well as disruption of vaginal flora and immune mediators may increase the risk of human immunodeficiency virus-1 acquisition. the objective of this study was to define immune changes in lower genital and systemic immunity associated with normal pregnancy. | 2013 | 23313311 |
| fetal membrane biomarker network diversity and disease functions induced by intra-amniotic pathogens. | intra-amniotic pathogens and by-products activate innate immune responses encompassing multitudes of signaling molecules and pathways that can result in spontaneous preterm birth (ptb). this study investigates fetal membrane response to bacterial stimulation using a bioinformatics approach. | 2013 | 23216633 |
| pseudoscardovia suis gen. nov., sp. nov., a new member of the family bifidobacteriaceae isolated from the digestive tract of wild pigs (sus scrofa). | seventeen fructose-6-phosphate phosphoketolase-positive bacterial strains were isolated from the digestive tract of wild pigs (sus scrofa). most of them were identified as bifidobacterium boum according to sequences of 16s rrna gene. two strains isolated from the small intestine content had unusual morphology of cells in comparison with bifidobacteria. cells growing in liquid anaerobic media were regular shaped rods arranged mostly in pairs. these isolates showed relatively low 16s rrna gene seq ... | 2013 | 23122702 |
| interactions between lactobacillus crispatus and bacterial vaginosis (bv)-associated bacterial species in initial attachment and biofilm formation. | certain anaerobic bacterial species tend to predominate the vaginal flora during bacterial vaginosis (bv), with gardnerella vaginalis being the most common. however, the exact role of g. vaginalis in bv has not yet been determined. the main goal of this study was to test the hypothesis that g. vaginalis is an early colonizer, paving the way for intermediate (e.g., fusobacterium nucleatum) and late colonizers (e.g., prevotella bivia). theoretically, in order to function as an early colonizer, spe ... | 2013 | 23739678 |
| adherence of bacteria to mucus collected from different parts of the reproductive tract of heifers and cows. | in the present study, we examined the adherence of indigenous vaginal bacteria, probiotic strains, and metritis pathogens to mucus collected from different parts of the reproductive tracts of heifers and cows and compared their adherence with the bacterial adherence to mucus collected from the stomach and large intestine of pigs. most of the vaginal strains adhered to mucus collected from different parts of the reproductive tract and strongly adhered to gastric mucus, with the exception of lacto ... | 2013 | 24206354 |
| bacterial modulation of human fetal membrane toll-like receptor expression. | preterm premature rupture of fetal membranes (pprom) occurs in 30-40% of spontaneous preterm births (ptb) and is associated with intra-amniotic infection and inflammation. the membranes may sense and respond to microbes via toll-like receptors (tlrs); however, little is known about their expression and regulation in this tissue. the objective of this study was to evaluate the expression of tlrs 1-10 in fetal membranes after exposure to pathogens associated with intra-amniotic infection and ptb. | 2013 | 22967004 |
| benzoyl peroxide formulated polycarbophil/carbopol 934p hydrogel with selective antimicrobial activity, potentially beneficial for treatment and prevention of bacterial vaginosis. | the human vagina is colonized by a variety of indigenous microflora; in healthy individuals the predominant bacterial genus is lactobacillus while those with bacterial vaginosis (bv) carry a variety of anaerobic representatives of the phylum actinobacteria. in this study, we evaluated the antimicrobial activity of benzoyl peroxide (bpo) encapsulated in a hydrogel against gardnerella vaginalis, one of the causative agents of bv, as well as indicating its safety for healthy human lactobacilli. her ... | 2013 | 24382940 |
| reconstitution of cholesterol-dependent vaginolysin into tethered phospholipid bilayers: implications for bioanalysis. | functional reconstitution of the cholesterol-dependent cytolysin vaginolysin (vly) from gardnerella vaginalis into artificial tethered bilayer membranes (tblms) has been accomplished. the reconstitution of vly was followed in real-time by electrochemical impedance spectroscopy (eis). changes of the eis parameters of the tblms upon exposure to vly solutions were consistent with the formation of water-filled pores in the membranes. it was found that reconstitution of vly is a strictly cholesterol- ... | 2013 | 24349307 |
| preserved ex vivo inflammatory status in decidual cells from women with preterm labor and subclinical intrauterine infection. | to compare the inflammatory response preserved ex vivo by decidual cells isolated from women who experienced preterm labor with and without subclinical intrauterine infection. | 2012 | 22928002 |
| amniotic fluid and maternal race influence responsiveness of fetal membranes to bacteria. | spontaneous preterm birth (ptb) and preterm prelabor rupture of membranes (pprom) occur more frequently in african-american women than in other racial groups. this may be due to an enhanced inflammatory response to pathogens associated with the condition. it is also possible that amniotic fluid (af) has different immunomodulatory properties in african-american women that increase their risk of ptb and pprom. to test this, we cultured fetal membranes from european-american and african-american wo ... | 2012 | 23021257 |
| pelvic inflammatory disease (pid) from chlamydia trachomatis versus pid from neisseria gonorrhea: from clinical suspicion to therapy. | pelvic inflammatory disease (pid) is the most significant complication of sexually transmitted infections in childbearing-age women and it represents an important public health problem because of its long-term sequelae (chronic pelvic pain, tubal infertility, ectopic pregnancy). prior to the mid 1970s pid was considered a monoetiologic infection, due primarily to neisseria gonorrhea. now it is well documented as a polymicrobial process, with a great number of microrganisms involved. in addition ... | 2012 | 23007248 |
| [gynophilus--a winning relay]. | the vagina is a complex biocenosis where many micro-organisms coexist and colonize it. the dominant colonizing bacteria of a healthy individual is of the genus "lactobacillus". it is also called doderlein's bacillus and determines the vaginal microbial balance through the production of lactic acid, hydrogen peroxide, biosurfactants, bacteriocines and modify the competition of pathogens for adhesion to the vaginal epithelial cells. through these mechanisms the lactobacilli block the growth and de ... | 2012 | 23236678 |
| effect of sexual debut on vaginal microbiota in a cohort of young women. | bacterial vaginosis is uncommon in women who are virgins. we estimated effects of sexual debut on vaginal bacterial colonization. | 2012 | 23168754 |
| in silico vs in vitro analysis of primer specificity for the detection of gardnerella vaginalis, atopobium vaginae and lactobacillus spp. | bacterial vaginosis (bv) is a common pathology of women in reproductive age that can lead to serious health complications, and is associated with shifts in the normal microflora from predominance of lactobacillus spp. to a proliferation of other anaerobes such as g. vaginalis and a vaginae, which can be detected by pcr. the optimal pcr pathogen detection assay relies mainly on the specificity and sensitivity of the primers used. | 2012 | 23153093 |
| longitudinal qpcr study of the dynamics of l. crispatus, l. iners, a. vaginae, (sialidase positive) g. vaginalis, and p. bivia in the vagina. | to obtain more detailed understanding of the causes of disturbance of the vaginal microflora (vmf), a longitudinal study was carried out for 17 women during two menstrual cycles. | 2012 | 23028904 |
| susceptibility testing of atopobium vaginae for dequalinium chloride. | atopobium vaginae and gardnerella vaginalis are major markers for bacterial vaginosis. we aimed to determine the mic and mbc range of the broad-spectrum anti-infective and antiseptic dequalinium chloride for 28 strains, belonging to 4 species of the genus atopobium, i.e. a. vaginae, a. minutum, a. rimae and a. parvulum. | 2012 | 22429611 |
| bacterial vaginosis (bv) candidate bacteria: associations with bv and behavioural practices in sexually-experienced and inexperienced women. | in recent years several new fastidious bacteria have been identified that display a high specificity for bv; however no previous studies have comprehensively assessed the behavioural risk associations of these bacterial vaginosis-candidate organisms (bv-cos). | 2012 | 22363457 |
| detection of bacterial 16s ribosomal rna genes for forensic identification of vaginal fluid. | to preliminarily evaluate the applicability of bacterial dna as a marker for the forensic identification of vaginal fluid, we developed and performed pcr-based detection of 16s ribosomal rna genes of lactobacillus spp. dominating the vagina and of bacterial vaginosis-related bacteria from dna extracted from body fluids and stains. as a result, 16s ribosomal rna genes of lactobacillus crispatus, lactobacillus jensenii and atopobium vaginae were specifically detected in vaginal fluid and female ur ... | 2012 | 22316585 |
| the natural antimicrobial peptide subtilosin acts synergistically with glycerol monolaurate, lauric arginate, and ε-poly-l-lysine against bacterial vaginosis-associated pathogens but not human lactobacilli. | subtilosin is a cyclical antimicrobial peptide produced by bacillus amyloliquefaciens that has antimicrobial activity against the bacterial vaginosis-associated human pathogen gardnerella vaginalis. the ability of subtilosin to inhibit g. vaginalis alone and in combination with the natural antimicrobial agents glycerol monolaurate (lauricidin), lauric arginate, and ε-poly-l-lysine was tested using a checkerboard approach. subtilosin was found to act synergistically with all of the chosen antimic ... | 2012 | 22252803 |