Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| Phenotypic characterization of Corynebacterium glutamicum using elementary modes towards synthesis of amino acids. | Elementary flux mode (EFM) analysis is a powerful tool to represent the metabolic network structure and can be further utilized for flux analysis. The method enables characterization and quantification of feasible phenotypes in microbes. EFM analysis was employed to characterize the phenotype of Corynebacterium glutamicum to yield various amino acids. The metabolic network of C. glutamicum yielded 62 elementary modes by incorporating the accumulation of amino acids namely, lysine, alanine, valin ... | 2011 | 22132055 |
| [The characteristics of Gram-positive bacteria isolated from skin lesions observed in ambulatory patients and the assessment of their susceptibility to antimicrobial drugs]. | The bacterial skin and soft-tissue infections occur commonly and are characterized by more or less intensified changes within skin and subcutaneous tissue. The bacterial skin infections give rise to significant therapeutic problems associated with increasing resistance etiological agents of these infections to antibiotics and chemotherapeutics. The aim of this study was to assess the distribution of various Gram-positive microorganisms in skin lesion observed in ambulatory patients in a period f ... | 2011 | 21853673 |
| [Drug sensitivity of causative agents in ocular infection of external adnexa and anterior segments--multicenter study of causative agents and drug sensitivity of ocular infection by the Japanese Association for Ocular Infection part II]. | To report the drug sensitivity of causative agents produced by ocular infection of external adnexa and anterior segments investigated by the nationwide survey conducted by Japanese Association for Ocular Infection between September, 2007 and August, 2008. | 2011 | 22073598 |
| genetic and biochemical characterization of corynebacterium glutamicum atp phosphoribosyltransferase and its three mutants resistant to feedback inhibition by histidine. | atp phosphoribosyltransferase (atp-prt) catalyzes the condensation of atp and prpp at the first step of histidine biosynthesis and is regulated by a feedback inhibition from product histidine. here, we report the genetic and biochemical characterization of such an enzyme, hisg(cg), from corynebacterium glutamicum, including site-directed mutagenesis of the histidine-binding site for the first time. gene disruption and complementation experiments showed that hisg(cg) is essential for histidine bi ... | 2011 | 22172596 |
| high sero-prevalence of caseous lymphadenitis identified in slaughterhouse samples as a consequence of deficiencies in sheep farm management in the state of minas gerais, brazil. | abstract: | 2011 | 22067701 |
| Corynebacterium epidermidicanis sp. nov., isolated from a dog's skin. | A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the skin of a dog, designated strain 410T, was subjected to comprehensive taxonomic characterization. Comparison of the 16S rRNA gene sequence showed highest similarities to Corynebacterium diphtheriae, Corynebacterium pseudotuberculosis, and Corynebacterium ulcerans (96.1-96.7%). The quinone system consisted predominantly of MK-8(H2) and MK-9(H2). The polar lipid profile of strain 410T contained the major compounds d ... | 2011 | 22081710 |
| Solution NMR structures reveal a distinct architecture and provide first structures for protein domain family PF04536. | The protein family (Pfam) PF04536 is a broadly conserved domain family of unknown function (DUF477), with more than 1,350 members in prokaryotic and eukaryotic proteins. High-quality NMR structures of the N-terminal domain comprising residues 41-180 of the 684-residue protein CG2496 from Corynebacterium glutamicum and the N-terminal domain comprising residues 35-182 of the 435-residue protein PG0361 from Porphyromonas gingivalis both exhibit an a/ß fold comprised of a four-stranded ß-sheet, thre ... | 2011 | 22198206 |
| Improving protein secretion of a transglutaminase-secreting Corynebacterium glutamicum recombinant strain on the basis of (13)C metabolic flux analysis. | Corynebacterium glutamicum is known as a host species for amino acid production. This microorganism was recently noticed as a host that produces secreted proteins. In this study, we performed (13)C metabolic flux analysis ((13)C-MFA) on a recombinant C. glutamicum strain that secretes a heterologous transglutaminase (TGase) to improve TGase secretion. For the (13)C-MFA of a TGase-secreting C. glutamicum strain in batch cultivation, a (13)C-labeling experiment and measurement of mass isotopomer d ... | 2011 | 21903468 |
| Corynebacterium glutamicum survives arsenic stress with arsenate reductases coupled to two distinct redox mechanisms. | Arsenate reductases (ArsCs) evolved independently as a defence mechanism against toxic arsenate. In the genome of Corynebacterium glutamicum, there are two arsenic resistance operons (ars1 and ars2) and four potential genes coding for arsenate reductases (Cg_ArsC1, Cg_ArsC2, Cg_ArsC1' and Cg_ArsC4). Using knockout mutants, in vitro reconstitution of redox pathways, arsenic measurements and enzyme kinetics, we show that a single organism has two different classes of arsenate reductases. Cg_ArsC1 ... | 2011 | 22032722 |
| lrp of corynebacterium glutamicum controls expression of the brnfe operon encoding the export system for l-methionine and branched-chain amino acids. | corynebacterium glutamicum possesses export systems for various amino acids including brnfe, a two-component export system for l-methionine and the branched-chain amino acids l-valine, l-isoleucine and l-leucine. a gene for a putative transcriptional regulator of the lrp family is transcribed divergently to the brnfe operon and is required for l-isoleucine export. by comparing global gene expression changes due to l-isoleucine addition we revealed increased brnfe expression in response to l-isol ... | 2011 | 21683740 |
| molecular characterization of corynebacterium pseudotuberculosis isolates using eric-pcr. | caseous lymphadenitis is an infectious sheep and goats disease caused by corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. c. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. the aim of this study is evaluating the potential of the enterobacterial repetitive intergenic consensus (eric-pcr) as a tool for molecular typing of c. pseudotuberculosis strains isolated in sh ... | 2011 | 21733644 |
| corynebacterium glutamicum rnase e/g-type endoribonuclease encoded by ncgl2281 is involved in the 5' maturation of 5s rrna. | corynebacterium glutamicum has one rnase e/g ortholog and one rnase j ortholog but no rnase y. we previously reported that the c. glutamicum ncgl2281 gene encoding the rnase e/g ortholog complemented the rng::cat mutation in escherichia coli but not the rne-1 mutation. in this study, we constructed an ncgl2281 knockout mutant and found that the mutant cells accumulated 5s rrna precursor molecules. the processing of 16s and 23s rrna, trna, and tmrna was normal. primer extension analysis revealed ... | 2011 | 21717142 |
| evaluation of the risk factors influencing the spread of caseous lymphadenitis in goat herds. | epidemiological studies on caseous lymphadenitis were carried out in poland in 1996 and 2002 among goat herds covered by a milk recording program. between-herd seroprevalence was 13.2% in 1996 and increased to 62.5% in 2002. the average size of seropositive herds was statistically significantly higher than that of seronegative ones, however there was no statistically significant difference in the age between the herds. a statistically significant prevalence ratio (pr) was identified and relevant ... | 2011 | 21721407 |
| infectious keratitis caused by fluoroquinolone-resistant corynebacterium. | 2011 | 21717177 | |
| Plasticity of Corynebacterium diphtheriae pathogenicity islands revealed by PCR. | Despite the existence of a vaccine against diphtheria, this disease remains endemic and is reemerging in several regions due to many factors, including variations in genes coding for virulence factors. One common feature of virulence factors is their high concentration in pathogenicity islands (PAIs), very unstable regions acquired via horizontal gene transfer, which has lead to the emergence of various bacterial pathogens. The 13 putative PAIs in Corynebacterium diphtheriae NCTC 13129 and the r ... | 2011 | 21732292 |
| biodegradation of specified risk material and characterization of actinobacterial communities in laboratory-scale composters. | as a result of bovine spongiform encephalopathy in canada, specific tissues at risk of harbouring prions are not allowed to enter the food chain. composting may be a viable alternative to rendering and land filling for the disposal of specified risk material (srm). two types of laboratory-scale composters, actively-heated and ambient systems were constructed to assess the biodegradation of srm over 30 days. a second heating cycle was generated by mixing the compost after 15 days. compared to amb ... | 2011 | 21360115 |
| efficient markerless gene replacement in corynebacterium glutamicum using a new temperature-sensitive plasmid. | random chemical mutation of a corynebacterium glutamicum-escherichia coli shuttle vector derived from plasmid pcgr2 was done using hydroxylamine. it brought about amino acid substitutions g109d and e180k within the replicase superfamily domain of the plasmid's repa protein and rendered the plasmid highly unstable, especially at higher incubation temperatures. colony formation of c. glutamicum was consequently completely inhibited at 37°c but not at 25°c. g109 is a semi-conserved residue mutation ... | 2011 | 21362445 |
| substrate specificity and ion coupling in the na+/betaine symporter betp. | betp is an na(+)-coupled betaine-specific transporter of the betaine-choline-carnitine (bcc) transporter family involved in the response to hyperosmotic stress. the crystal structure of betp revealed an overall fold of two inverted structurally related repeats (leut-fold) that betp shares with other sequence-unrelated na(+)-coupled symporters. numerous structures of leut-fold transporters in distinct conformational states have contributed substantially to our understanding of the alternating acc ... | 2011 | 21364531 |
| deoxycytidine production by metabolically engineered corynebacterium ammoniagenes. | corynebacterium ammoniagenes n424 was metabolically modified to isolate overproducers of deoxycytidine. inosine auxotrophy (ino) was initially introduced to prevent the flow of prpp (phosphoribosyl pyrophosphate) into the purine biosynthetic pathway by random mutagenesis using n-methyl-n'-nitro-n-nitrosoguanidine. following that, mutants possessing hydroxyurea resistance (hu(r)) were isolated to increase the activity of ribonucleoside diphosphate reductase, which catalyzes the reduction of ribon ... | 2011 | 21369979 |
| mixed glucose and lactate uptake by corynebacterium glutamicum through metabolic engineering. | the corynebacterium glutamicum atcc 13032 lysc(fbr) strain was engineered to grow fast on racemic mixtures of lactate and to secrete lysine during growth on lactate as well as on mixtures of lactate and glucose. the wild-type c. glutamicum only grows well on l-lactate. overexpression of d-lactate dehydrogenase (dld) achieved by exchanging the native promoter of the dld gene for the stronger promoter of the sod gene encoding superoxide dismutase in c. glutamicum resulted in a duplication of bioma ... | 2011 | 21370474 |
| bronchoalveolar lavage in the diagnosis of ventilator-associated pneumonia: to quantitate or not, that is the question. | quantitative bronchoalveolar lavage (bal) is used to diagnose ventilator-associated pneumonia (vap). we prospectively compared semiquantitative (sq) and quantitative (qu) culture of bal for vap diagnosis. ventilated patients suspected of vap underwent bronchoscopic bal. bal fluid was examined by both qu (colony-forming units [cfus]/ml) and sq culture (none, sparse, moderate, or heavy) and results were compared. vap was defined as 105 cfu/ml or greater on qu culture. over 36 months, 319 bals were ... | 2011 | 21375840 |
| variability and diversity of nasopharyngeal microbiota in children: a metagenomic analysis. | the nasopharynx is the ecological niche for many commensal bacteria and for potential respiratory or invasive pathogens like streptococcus pneumoniae, haemophilus influenzae, and neisseria meningitidis. disturbance of a balanced nasopharyngeal (np) microbiome might be involved in the onset of symptomatic infections with these pathogens, which occurs primarily in fall and winter. it is unknown whether seasonal infection patterns are associated with concomitant changes in np microbiota. as young c ... | 2011 | 21386965 |
| exogenous coproporphyrin iii production by corynebacterium aurimucosum and microbacterium oxydans in erythrasma lesions. | erythrasma is a superficial skin disease caused by gram-positive corynebacterium species. coral-red fluorescence under wood's light, strongly suggestive of erythrasma, can be attributed to the presence of porphyrins. fractionated porphyrin analysis in erythrasma lesions is yet to be reported. we attempted to investigate erythrasma lesions by isolating the responsible bacteria and determining their exogenous porphyrin production by hplc analysis. we observed a 78-year-old woman with erythrasma wh ... | 2011 | 21393451 |
| corynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goats. | in the current study, the applicability of the quantification of gamma interferon (ifn-+¦) levels for the detection of animals infected with corynebacterium pseudotuberculosis and for determining caseous lymphadenitis (cla) clinical status was evaluated. peripheral blood leukocytes were collected from cla nonendemic areas animals, from cla seropositive animals without clinical signs of the disease, and from seropositive animals presenting cla clinical signs. the leukocytes were stimulated with c ... | 2011 | 21398439 |
| dynamics of indigenous bacterial communities associated with crude oil degradation in soil microcosms during nutrient-enhanced bioremediation. | bacterial population dynamics were examined during bioremediation of an african soil contaminated with arabian light crude oil and nutrient enrichment (biostimulation). polymerase chain reaction followed by denaturing gradient gel electrophoresis (dgge) were used to generate bacterial community fingerprints of the different treatments employing the 16s ribosomal ribonucleic acid (rrna) gene as molecular marker. the dgge patterns of the nutrient-amended soils indicated the presence of distinguish ... | 2011 | 21824988 |
| [characteristics of microbiocenosis of gingival pocket in patients with chronic generalized periodontitis]. | as a result of examination of 56 patients with chronic generalized periodontitis (cgp) of a moderate severity and a severe form of the disease, microflora from gingival pocket in patients which severe form of cgp was established to have higher specific weight in index of dissemination of strains such as staphylococcus aureus, streptococcus pyogencs, enterococcus, corynebacterium spp., anaerobic bacteria. strains from patients with a severe form of cgp were characterized by a higher level of expr ... | 2011 | 21716238 |
| strain-dependent arthritogenic potential of the zoonotic pathogen corynebacterium ulcerans. | during the last decade the majority of diphtheria cases in europe had corynebacterium ulcerans as the etiologic agent with dogs and cats as the reservoir hosts. however, little has been documented about the virulence factors of this zoonotic pathogen. to set up an in vivo experimental c. ulcerans infection model, conventional swiss webster mice were intravenously infected with different doses (from 1+ù10(7) to 5+ù10(9) bacteria per mouse) of c. ulcerans strains, namely 809 (from human lower resp ... | 2011 | 21742447 |
| genome sequence of corynebacterium nuruki s6-4t, isolated from alcohol fermentation starter. | corynebacterium nuruki s6-4(t), isolated from korean alcohol fermentation starter, is a strictly aerobic, nonmotile, gram-positive, and rod-shaped bacterium belonging to the genus corynebacterium and the actinomycete group. we report here the draft genome sequence of c. nuruki strain s6-4(t) (3,106,595 bp, with a g+c content of 69.5%). | 2011 | 21685278 |
| synthesis of +¦-aminobutyric acid by expressing lactobacillus brevis-derived glutamate decarboxylase in the corynebacterium glutamicum strain atcc 13032. | purpose of work: purpose of this work is to synthesize +¦-aminobutyric acid by glutamate-producing species expressing lactobacillus brevis-derived glutamate decarboxylase genes, i.e. recombinant corynebacterium glutamicum strains, which directly convert endogenous l: -glutamate precursor into +¦-aminobutyric acid (gaba) through single-step fermentation. to express exogenous glutamate decarboxylase (gad) in an l: -glutamate-producing strain, lactobacillus brevis lb85, which can produce gaba, was ... | 2011 | 21826397 |
| the role of trimerization in the osmoregulated betaine transporter betp. | the osmoregulated betaine transporter betp is a stable trimer. structural studies have shown that individual protomers can adopt distinct transport conformations, implying a functional role for the trimeric state in transport, although the role of trimerization in regulation is not yet understood. we designed putative monomeric mutants by molecular-dynamics simulations and in silico alanine-scanning mutagenesis. several mutants including betp-w101a/t351a were monomeric in detergent as well as in ... | 2011 | 21681199 |
| mycobacterium tuberculosis acyl carrier protein synthase adopts two different ph-dependent structural conformations. | the crystal structures of acyl carrier protein synthase (acps) from mycobacterium tuberculosis (mtb) and corynebacterium ammoniagenes determined at ph 5.3 and ph 6.5, respectively, are reported. comparison of the mtb apo-acps structure with the recently reported structure of the mtb acps-adp complex revealed that acps adopts two different conformations: the orthorhombic and trigonal space-group structures show structural differences in the +¦2 helix and in the conformation of the +¦3-+¦4 connect ... | 2011 | 21697604 |
| luminescent rhenium(i) polypyridine complexes appended with an +¦-d-glucose moiety as novel biomolecular and cellular probes. | 2011 | 21714023 | |
| metabolic engineering of cellular transport for overproduction of the platform chemical 1,5-diaminopentane in corynebacterium glutamicum. | the present work describes the development of a superior strain of corynebacterium glutamicum for diaminopentane (cadaverine) production via metabolic engineering of cellular transport processes. in c. glutamicum dap-3c, a tailor-made producer, the diaminopentane forming enzyme, lysine decarboxylase, was inhibited in vivo by its end-product, suggesting a potential bottleneck at the level of the export. the previously proposed lysine exporter lyse was shown not to be involved in diaminopentane ex ... | 2011 | 21821142 |
| surface microbial consortia from livarot, a french smear-ripened cheese. | the surface microflora (902 isolates) of livarot cheeses from three dairies was investigated during ripening. yeasts were mainly identified by fourier transform infrared spectroscopy. geotrichum candidum was the dominating yeast among 10 species. bacteria were identified using biotype 100 strips, dereplicated by repetitive extragenic palindromic pcr (rep-pcr); 156 representative strains were identified by either box-pcr or (gtg)(5)-pcr, and when appropriate by 16s rdna sequencing and sds-page an ... | 2011 | 21815832 |
| enteral tube feeding alters the oral indigenous microbiota in elderly adults. | enteral tube feeding is widely used to maintain nutrition for elderly adults with eating difficulties, but its long-term use alters the environment of the oral ecosystem. this study characterized the tongue microbiota of tube-fed elderly adults by analyzing the 16s rrna gene. the terminal restriction fragment length polymorphism (t-rflp) profiles of 44 tube-fed subjects were compared with those of 54 subjects fed orally (average age 86.4 -¦ 6.9 years). barcoded pyrosequencing data were also obta ... | 2011 | 21821752 |
| expression, purification, crystallization and preliminary crystallographic analysis of cg1458: a novel oxaloacetate decarboxylase from corynebacterium glutamicum. | oxaloacetate decarboxylase catalyses the decarboxylation of oxaloacetate to pyruvate and co(2). recently, the corynebacterium glutamicum gene product cg1458 was determined to be a soluble oxaloacetate decarboxylase. to elucidate the mechanism of oxaloacetate decarboxylation by cg1458, recombinant cg1458 was purified and crystallized. the best crystal was grown from 0.2ôçàm mgcl(2), 0.1ôçàm bis-tris ph 6.0, 25%(w/v) polyethylene glycol 3350 using the hanging-drop method. the crystals belonged to ... | 2011 | 21821907 |
| concurrent diphtheria and infectious mononucleosis: difficulties for management, investigation and control of diphtheria in developing countries. | we report a case of concurrent diphtheria and infectious mononucleosis in a brazilian 11-year-old child. two days after started the specific treatment for diphtheria the case was discharged following clinical recovery. this case highlights the difficulties to diagnose diphtheria clinically in partially immunized individuals and for management and control of diphtheria in developing countries. | 2011 | 21680765 |
| homology modeling, molecular dynamics and qm/mm study of the regulatory protein phop from corynebacterium pseudotuberculosis. | corynebacterium pseudotuberculosis is a facultatively intracellular gram-positive bacterium that causes caseous lymphadenitis, principally in sheep and goats, though sometimes in other species of animals, leading to considerable economic losses. this pathogen has a tcs known as phopr, which consists of a sensory histidine kinase protein (phor) and an intracellular response regulator protein (phop). this system is involved in the regulation of proteins present in various processes, including viru ... | 2011 | 21701810 |
| comparison between elementary flux modes analysis and 13c-metabolic fluxes measured in bacterial and plant cells. | abstract: | 2011 | 21682932 |
| detection of bacteria in normal adult nasal cavity based on polymerase chain reaction-denaturing gradient gel electrophoresis. | this study investigated the bacterial diversity in a normal adult nasal cavity using polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) of 16s ribosomal rna (rrna) gene fragments and compared the results with those of a culture-based method. | 2011 | 21711966 |
| corynebacterium ulcerans from diseased wild boars. | two corynebacterium strains were isolated from lymph nodes of wild boars showing severe alterations caused by caseous lymphadenitis. the wild boars came from different districts in southern germany; one was found dead, the other had been shot. the two corynebacterium strains obtained were both positive for phospholipase d. further analysis of biochemical profiles did not allow unambiguous differentiation between corynebacterium ulcerans and corynebacterium pseudotuberculosis. fourier-transformed ... | 2011 | 21824349 |
| identification of corynebacterium pseudotuberculosis from sheep by pcr-restriction analysis using the rna polymerase ß-subunit gene (rpob). | caseous lymphadenitis, caused by corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including argentina and brazil. a polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic rna polymerase ß-subunit gene (rpob). all available corynebacteriumrpob sequences were analyzed by computer-assisted restriction analysis. the rpob p ... | 2011 | 21419467 |
| identification of succinate exporter in corynebacterium glutamicum and its physiological roles under anaerobic conditions. | corynebacterium glutamicum produces succinate from glucose via the reductive tricarboxylic acid cycle under microaerobic and anaerobic conditions. we identified a ncgl2130 gene of c. glutamicum as a novel succinate exporter that functions in succinate production, and designated suce1. suce1 expression levels were higher under microaerobic conditions than aerobic conditions, and overexpression or disruption of suce1 respectively increased or decreased succinate productivity during fermentation. u ... | 2011 | 21420450 |
| targeted allele replacement mutagenesis of corynebacterium pseudotuberculosis. | a two-step allele replacement mutagenesis procedure, using a conditionally replicating plasmid, was developed to allow the creation of targeted, marker-free mutations in corynebacterium pseudotuberculosis. the relationship between homologous sequence length and recombination frequency was determined, and enhanced plasmid excision was observed due to the rolling-circle replication of the mutagenesis vector. furthermore, an antibiotic enrichment procedure was applied to improve the recovery of mut ... | 2011 | 21421779 |
| additive effect of rpb27 immunization and chemotherapy in experimental paracoccidioidomycosis. | paracoccidioidomycosis, pcm, the major systemic mycosis in latin america, is caused by the termally dimorphic fungus paracoccidioides brasiliensis and requires extended periods of chemotherapy with a significant frequency of relapsing disease. the search for new alternatives of treatment is necessary. rpb27 is an antigenic protein from p. brasiliensis that already showed a significant protective activity as a vaccine for pcm in experimental models. the cdna of rpb27 was subcloned into a pet-dest ... | 2011 | 21423771 |
| metabolic engineering of 1,2-propanediol pathways in corynebacterium glutamicum. | we analyzed 1,2-propanediol (1,2-pd) production in metabolically engineered corynebacterium glutamicum. wild-type c. glutamicum produced 93 µm 1,2-pd after 132 h incubation under aerobic conditions. no gene encoding the methylglyoxal synthase (mgs) which catalyzes the first step of 1,2-pd synthesis from the glycolytic pathway was detected on the c. glutamicum genome, but several genes annotated as encoding putative aldo-keto reductases (akrs) were present. akr functions as a methylglyoxal reduct ... | 2011 | 21424269 |
| detection of ascitic fluid infections in patients with liver cirrhosis and ascites. | ascitic fluid infections (afis) are the frequent complications of advanced liver disease. bacterial translocation is considered a key step in the pathogenesis of gut-derived bacterial infections; mainly spontaneous bacterial peritonitis (sbp) in cirrhotic patients. bacterial dna (bactdna) in ascitic fluid and serum has been suggested as a surrogate marker for bacterial translocation. we attempted at the isolation and identification of bacteria in ascitic fluid in cirrhotic patients and the asses ... | 2011 | 21429450 |
| ovine pedomics: the first study of the ovine foot 16s rrna-based microbiome. | we report the first study of the bacterial microbiome of ovine interdigital skin based on 16s rrna by pyrosequencing and conventional cloning with sanger-sequencing. three flocks were selected, one a flock with no signs of footrot or interdigital dermatitis, a second flock with interdigital dermatitis alone and a third flock with both interdigital dermatitis and footrot. the sheep were classified as having either healthy interdigital skin (h) and interdigital dermatitis (id) or virulent footrot ... | 2011 | 21430786 |
| lipoarabinomannan biosynthesis in corynebacterineae: the interplay of two +¦(1ôåæ2)-mannopyranosyltransferases mptc and mptd in mannan branching. | lipomannan (lm) and lipoarabinomannan (lam) are key corynebacterineae glycoconjugates that are integral components of the mycobacterial cell wall, and are potent immunomodulators during infection. lam is a complex heteropolysaccharide synthesized by an array of essential glycosyltransferase family c (gt-c) members, which represent potential drug targets. herein, we have identified and characterized two open reading frames from corynebacterium glutamicum that encode for putative gt-cs. deletion o ... | 2011 | 21435038 |
| urethritis due to corynebacterium glucuronolyticum. | we describe a case of urethritis in a young man caused by corynebacterium glucuronolyticum. this bacterium is a synonym of corynebacterium seminale, a known agent of non-gonococcal urethritis, that cannot be regarded as commensal flora in the urogenital region when is isolated in a symptomatic clinical context. accuracy in diagnosis and correct treatment is important for avoiding probable complications, for example prostatitis. bacterial isolation is convenient, and an antimicrobial susceptibili ... | 2011 | 21437677 |
| an artificial pathway to 3,4-dihydroxybenzoic acid allows generation of new aminocoumarin antibiotic recognized by catechol transporters of e. coli. | an artificial operon was synthesized, consisting of the genes for chorismate pyruvate-lyase of e. coli and for 4-hydroxybenzoate 3-hydroxylase of corynebacterium cyclohexanicum. this operon, directing the biosynthesis of 3,4-dihdroxybenzoate, was expressed in the heterologous expression host streptomyces coelicolor m512, together with a modified biosynthetic gene cluster for the aminocoumarin antibiotic clorobiocin. the resulting strain produced a clorobiocin derivative containing a 3,4-dihdroxy ... | 2011 | 21439475 |
| corynebacterium glutamicum tailored for efficient isobutanol production. | we recently engineered corynebacterium glutamicum for aerobic production of 2-ketoisovalerate by inactivation of the pyruvate dehydrogenase complex, pyruvate:quinone oxidoreductase, transaminase b, and additional overexpression of the ilvbncd genes, encoding acetohydroxyacid synthase, acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. based on this strain, we engineered c. glutamicum for the production of isobutanol from glucose under oxygen deprivation conditions by inactivation ... | 2011 | 21441331 |
| a novel comparative genomics analysis for common drug and vaccine targets in corynebacterium pseudotuberculosis and other cmn group of human pathogens. | caseous lymphadenitis is a chronic goat and sheep disease caused by corynebacterium pseudotuberculosis (cp) that accounts for a huge economic loss worldwide. proper vaccination or medication is not available because of the lack of understanding of molecular biology of the pathogen. in a recent approach, four cp (cpfrc41, cp1002, cpc231, and cpi-19) genomes were sequenced to elucidate the molecular pathology of the bacteria. in this study, using these four genome sequences along with other eight ... | 2011 | 21443692 |
| images in clinical medicine. coral-red fluorescence. | 2011 | 21449776 | |
| identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in corynebacterium glutamicum. | corynebacterium glutamicum uses the phosphoenolpyruvate-dependent sugar phosphotransferase system (pts) to uptake and phosphorylate glucose; no other route has yet been identified. disruption of the ptsh gene in wild-type c. glutamicum resulted, as expected, in a phenotype exhibiting little growth on any of the pts sugars: glucose, fructose, and sucrose. however, a suppressor mutant that grew on glucose but not on the other two sugars was spontaneously isolated from the pts-negative strain wt?pt ... | 2011 | 21452034 |
| the ncgl1108 (phep (cg)) gene encodes a new l: -phe transporter in corynebacterium glutamicum. | corynebacterium glutamicum played a central role in the establishment of fermentative production of amino acids, and it is a model for genetic and physiological studies. the general aromatic amino acid transporter, arop( cg ), was the sole functionally identified aromatic amino acid transporter from c. glutamicum. in this study, the ncgl1108 (named as phep ( cg )), which is located upstream of the genetic cluster (ncgl1110 ∼ ncgl1113) for resorcinol catabolism, was identified as a new l: -phe sp ... | 2011 | 21468701 |
| nirk and nirs nitrite reductase genes from non-agricultural forest soil bacteria in thailand. | the genetic heterogeneity of the nitrite reductase gene (nirk and nirs) fragments from denitrifying prokaryotes in a non-agricultural forest soil in thailand was investigated using soil samples from the plant germplasm-royal initiation project area in kanchanaburi province, thailand. soil bacteria were screened for denitrification activity and 13 (from 211) positive isolates were obtained and further evaluated for their ability to reduce nitrate and to accumulate or reduce nitrite. three species ... | 2011 | 21475726 |
| [corynebacterium striatum pneumonia in an hiv patient.] | 2011 | 21477896 | |
| phosphotransferase system-independent glucose utilization in corynebacterium glutamicum by inositol permeases and glucokinases. | phosphoenolpyruvate-dependent glucose phosphorylation via the phosphotransferase system (pts) is the major path of glucose uptake in corynebacterium glutamicum, but some growth from glucose is retained in the absence of the pts. the growth defect of a deletion mutant lacking the general pts component hpr in glucose medium could be overcome by suppressor mutations leading to the high expression of inositol utilization genes or by the addition of inositol to the growth medium if a glucokinase is o ... | 2011 | 21478323 |
| simple dual-spotting procedure enhances nlc-maldi ms/ms analysis of digests with less specific enzymes. | the beneficial effect of high mass accuracy in mass spectrometry is especially pronounced when using less specific enzymes as the number of theoretically possible peptides increases dramatically without any cleavage specificity defined. together with a preceding chromatographic separation, high-resolution mass spectrometers such as the maldi-ltq-orbitrap are therefore well suited for the analysis of protein digests with less specific enzymes. a combination with fast, automated, and informative m ... | 2011 | 21480673 |
| host associated polymorphisms in the corynebacterium pseudotuberculosis rpob gene sequence. | corynebacterium pseudotuberculosis is a widespread facultative intracellular pathogen that causes caseous lymphadenitis disease in sheep and goats, and generates cutaneous abscesses and granulomas in horses and cattle. although some genes have been studied for diagnostic and phylogenetic analysis within the genus corynebacterium, at subspecies level the pathogen has been poorly analyzed. the aim of this study was to characterize c. pseudotuberculosis strains isolated from domestic animals, throu ... | 2011 | 21482046 |
| bacteriologic and plasmid analysis of etiologic agents of conjunctivitis in lagos, nigeria. | background: conjunctivitis, an inflammation of the conjunctiva, is one of the most common eye problems affecting all age groups in nigeria. a better understanding of its epidemiology and the antibiotic susceptibility of etiologic bacterial agents is crucial for the initiation of preventive and therapeutic measures. this study determined the distribution and patterns of bacterial infections in nigerian patients with conjunctivitis. antibiotic resistance patterns and the plasmid profiles of these ... | 2011 | 21484175 |
| novel polyoxyethylene-containing glycolipids are synthesized in corynebacterium matruchotii and mycobacterium smegmatis cultured in the presence of tween 80. | the addition of polyoxyethylene sorbitan monooleate (tween 80) to a culture of mycobacteria greatly influences cell permeability and sensitivity to antibiotics but very little is known regarding the underlying mechanism. here we show that corynebacterium matruchotii (surrogate of mycobacteria) converts tween 80 to a structural series of polyoxyethylenic acids which are then used to form novel series-2a and series-2b glycolipids. minor series-3 glycolipids were also synthesized. the polyoxyethyle ... | 2011 | 21490808 |
| [development of an elisa test to detect antibodies in vaccinated sheep or infected corynebacterium pseudotuberculosis]. | the aim of this study was to evaluate an indirect specific elisa developed for the detection of humoral immune response in vaccinated sheep and/or challenged with a corynebacterium pseudotuberculosis strain. healthy 4 month-old lambs were distributed into 4 groups: group 1 immunized (g1, n = 5), group 2 vaccinated/inoculated (g2, n = 8), group 3 inoculated (g3, n = 2) and group 4 control (g4, n = 2). groups g1 and g2 received two doses of an experimental bacterin. four weeks postvaccination, g2 ... | 2011 | 21491060 |
| dna repair in corynebacterium model. | corynebacterium spp. are a group of gram-positive bacteria that includes plant and animal pathogens, nonpathogenic soil bacteria, and saprophytic species. our understanding of these organisms is still poor compared with that of other bacterial organisms, but new insights offered by genome sequence data and the elucidation of gene content has provided clues about the nature, genome stability, pathogenicity and virulence of these organisms. we compared 15 corynebacterium genomes, from pathogenic a ... | 2011 | 21497183 |
| investigation of phosphorylation status of odhi protein during penicillin- and tween 40-triggered glutamate overproduction by corynebacterium glutamicum. | glutamate overproduction by corynebacterium glutamicum is triggered by treatment with penicillin or tween 40 and is accompanied by a decrease in 2-oxoglutarate dehydrogenase complex (odhc) activity. we have reported that de novo synthesis of odhi, which inhibits odhc activity by interacting specifically with the e1o subunit of odhc (odha), is induced by penicillin, and that odhi overexpression induces glutamate overproduction in the absence of any triggers for glutamate overproduction. in this s ... | 2011 | 21503757 |
| immunology and probiotic impact of the newborn and young children intestinal microflora. | human body has developed a holistic defence system, which mission is either to recognize and destroy the aggressive invaders or to evolve mechanisms permitting to minimize or restore the consequences of harmful actions. the host immune system keeps the capital role to preserve the microbial intestinal balance via the barrier effect. specifically, pathogenic invaders such as, bacteria, parasites, viruses and other xenobiotic invaders are rejected out of the body via barriers formed by the skin, m ... | 2011 | 21515397 |
| tools for genetic manipulations in corynebacterium glutamicum and their applications. | corynebacterium glutamicum is an important industrial producer of various amino acids with great potential for the production of other metabolites. the complete genome sequences of two c. glutamicum strains were determined and the use of genome-based approaches (transcriptomics, proteomics, metabolomics, and fluxomics) provided large amounts of data on the metabolism of this bacterium and its regulation. many tools for genetic manipulations in c. glutamicum have been developed and used for the a ... | 2011 | 21519933 |
| lipoarabinomannan and related glycoconjugates: structure, biogenesis and role in mycobacterium tuberculosis physiology and host-pathogen interaction. | approximately one third of the world's population is infected with mycobacterium tuberculosis, the causative agent of tuberculosis. this bacterium has an unusual lipid-rich cell wall containing a vast repertoire of antigens, providing a hydrophobic impermeable barrier against chemical drugs, thus representing an attractive target for vaccine and drug development. apart from the mycolyl-arabinogalactan-peptidoglycan complex, mycobacteria possess several immunomodulatory constituents, notably lipo ... | 2011 | 21521247 |
| high yield secretion of heterologous proteins in corynebacterium glutamicum using its own tat-type signal sequence. | efficient protein secretion, the basis of large-scale production of many compounds central to the biotechnology industry, is achieved by signal peptide and propeptide optimization in addition to optimizing host factors affecting heterologous protein production. here, we fused green fluorescent protein (gfp) to the recently identified tat-type secretory signal peptide of cgr0949 to demonstrate a high-yield protein secretion system of corynebacterium glutamicum. the resultant secretion vector faci ... | 2011 | 21523478 |
| cutaneous diphtheria in the urban poor population of vancouver, british columbia: a 10-year review. | between 1998 - 2007, records from 33 patients with cutaneous diphtheria from vancouver's inner-city were reviewed. cases were associated with injection drug use and poverty. co-infections with staphylococcus aureus, streptococcus pyogenes and arcanobacterium haemolyticum occurred. corynebacterium diphtheriae is endemic in vancouver's urban core, with mlst sequence type 76 strains predominating. | 2011 | 21525220 |
| treatment with daptomycin for corynebacterium jeikeium left-sided prosthetic valve endocarditis. | prosthetic valve endocarditis (pve) is a serious complication with potential fatal consequences, classified as early or late pve, depending on whether typical symptoms occur within or later than 12 months from surgery. the incidence of early pve is under 1%, but it carries high morbidity and mortality rates. there are few reported cases in literature of pve due to corynebacterium jeikeium even though it is present in normal skin flora particularly in hospitalized patients. corynebacterium specie ... | 2011 | 21525826 |
| [bacteriological quality of air in a ward for sterile pharmaceutical preparations]. | an extremely clean area is required for preparation of sterile pharmaceutical compounds, in compliance with international standards, to minimize the probability of microbial contamination. | 2011 | 21526280 |
| co-evolutionary analysis enables rational deregulation of allosteric enzyme inhibition in corynebacterium glutamicum for lysine production. | product feedback inhibition of allosteric enzymes is an essential issue for developing highly efficient microbial strains for bioproduction. here we use aspartokinase from corynebacterium glutamicum (cgak), a key enzyme controlling the biosynthesis of industrially important aspartate family amino acids, as a model to demonstrate a fast and efficient approach to deregulate allostery. in the last fifty years many researchers and companies have made considerable efforts to deregulate this enzyme fr ... | 2011 | 21531824 |
| microbial contamination of autologous peripheral blood stem cell products: incidence, clinical outcome, quality control and management strategies. | : microbial screening is part of quality control for autologous peripheral blood stem cell (pbsc) manipulation. the incidence of culture positive products varies widely. the clinical significance of infusing culture positive products is uncertain, with no consensus on the interventions required. | 2011 | 21532522 |
| evidence for reductive genome evolution and lateral acquisition of virulence functions in two corynebacterium pseudotuberculosis strains. | corynebacterium pseudotuberculosis, a gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (cl). cl mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. this species is distributed worldwide, but it has the most serious economic impact in oceania, africa and south america. although c. pseudotuberculosis causes major health and productivity problems for livestock, little is k ... | 2011 | 21533164 |
| engineering bacillus subtilis for isobutanol production by heterologous ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression. | in the present work, bacillus subtilis was engineered as the cell factory for isobutanol production due to its high tolerance to isobutanol. initially, an efficient heterologous ehrlich pathway controlled by the promoter p(43) was introduced into b. subtilis for the isobutanol biosynthesis. further, investigation of acetolactate synthase of b. subtilis, ketol-acid reductoisomerase, and dihydroxy-acid dehydratase of corynebacterium glutamicum responsible for 2-ketoisovalerate precursor biosynthes ... | 2011 | 21533914 |
| using a starch-rich mutant of arabidopsis thaliana as feedstock for fermentative hydrogen production. | a mutant plant (arabidopsis thaliana), sex1-1 (starch excess 1-1), accumulating high starch content in leaves was created to serve as better biomass feedstock for a h(2)-producing strain clostridium butyricum cgs2, which efficiently utilizes starch for h(2) production but cannot assimilate cellulosic materials. the starch content of the mutant plant increased to 10.67mg/fresh weight, which is four times higher than that of wild type plant. using sex1-1 mutant plant as feedstock, c. butyricum cgs ... | 2011 | 21546247 |
| an alternative method for purifying and detoxifying diphtheria toxin. | infections caused by corynebacterium diphtheriae frequently induce situations in which very small doses of antigens injected intradermally can cause strong inflammatory reactions. this bacterium secretes the diphtheria toxin (dt), a virulence factor that can be lethal to the human organism at doses below 0.1 μg/kg of body weight. the present work proposes alternative methods of dt purification using affinity chromatography and of dt detoxification through conjugating with the polymer methoxypoly ... | 2011 | 21549738 |
| biotechnological production of polyamines by bacteria: recent achievements and future perspectives. | in bacteria, the pathways of polyamine biosynthesis start with the amino acids l: -lysine, l: -ornithine, l: -arginine, or l: -aspartic acid. some of these polyamines are of special interest due to their use in the production of engineering plastics (e.g., polyamides) or as curing agents in polymer applications. at present, the polyamines for industrial use are mainly synthesized on chemical routes. however, since a commercial market for polyamines as well as an industry for the fermentative pro ... | 2011 | 21552989 |
| adhesion by pathogenic corynebacteria. | pathogenic members of the genus corynebacterium cause a wide range of serious infections in humans including diphtheria. adhesion to host cells is a crucial step during infection. in corynebacterium diphtheriae, adhesion is mediated primarily by filamentous structures called pili or fimbriae that are covalently attached to the bacterial cell wall. c. diphtheriae produces three distinct pilus structures, spaa-, spad- and spah-type pili. similar to other types, the prototype spaa pilus consists of ... | 2011 | 21557059 |
| crystal structures of the transcriptional repressor rolr reveals a novel recognition mechanism between inducer and regulator. | many members of the tetr family control the transcription of genes involved in multidrug resistance and pathogenicity. rolr (resorcinolregulator), the recently reported tetr-type regulator for aromatic catabolism from corynebacterium glutamicum, distinguishes itself by low sequence similarities and different regulation from the previously known members of the tetr family. here we report the crystal structures of rolr in its effector-bound (with resorcinol) and aop- forms at 2.5 å and 3.6 å, resp ... | 2011 | 21559286 |
| purification and characterization of an arginine regulatory protein, argr, in corynebacterium glutamicum. | corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. we previously showed that, in c. glutamicum, argcjbdfrgh arginine biosynthesis genes are clustered but independently transcribed from argc and argg promoters, leading to the generation of two transcripts corresponding to argcjbdfr and arggh. in this report, we show the effect of the c. glutamicum argr repressor on argc and argg promoters by overexpressing or disrupting the argr gene. ... | 2011 | 21559975 |
| antioxidant, antihypertensive and antimicrobial properties of ovine milk caseinate hydrolyzed with a microbial protease. | background: bioactive peptides might be released from precursor proteins through enzymatic hydrolysis. these molecules could be potentially employed in health and food products. in this investigation, ovine milk caseinate hydrolysates obtained with a novel microbial protease derived from bacillus sp. p7 were evaluated for antioxidant, antimicrobial, and angiotensin i-converting enzyme (ace)-inhibitory activities. results: antioxidant activity measured by the 2,2'-azino-bis-(3-ethylbenzothiazolin ... | 2011 | 21560133 |
| kinetic characterisation of recombinant corynebacterium glutamicum nad(+)-dependent ldh over-expressed in e. coli and its rescue of an lldd (-) phenotype in c. glutamicum: the issue of reversibility re-examined. | the ldh gene of corynebacterium glutamicum atcc 13032 (gene symbol cg3219, encoding a 314 residue nad(+)-dependent l: -(+)-lactate dehydrogenase, ec 1.1.1.27) was cloned into the expression vector pkk388-1 and over-expressed in an ldha-null e. coli tg1 strain upon isopropyl-β-d-thiogalactopyranoside (iptg) induction. the recombinant protein (referred to here as cgldh) was purified by a combination of dye-ligand and ion-exchange chromatography. though active in its absence, cgldh activity is enha ... | 2011 | 21567176 |
| infective endocarditis caused by arcanobacterium haemolyticum: a case report. | abstract: arcanobacterium haemolyticum is an organism that commonly causes pharyngitis and wound infections. it does not usually cause systemic invasive disease. the organism presents a difficult diagnostic problem because the clinical microbiology laboratory has a propensity to view them as diphtheroid organisms of the corynebacterium species, thus contaminants or normal flora. we describe a case of a 21-year-old female who had endocarditis with cerebral emboli due to arcanobacterium haemolytic ... | 2011 | 21569379 |
| diversity of bacteria at healthy human conjunctiva. | purpose. ocular surface (os) microbiota contributes to infectious and autoimmune diseases of the eye. comprehensive analysis of microbial diversity at the os has been impossible because of the limitations of conventional cultivation techniques. this pilot study aimed to explore true diversity of human os microbiota using dna sequencing-based detection and identification of bacteria. methods. composition of the bacterial community was characterized using deep sequencing of the 16s rrna gene ampli ... | 2011 | 21571682 |
| corynebacterium deserti sp. nov., isolated from desert in the west of china. | a novel coryneform bacterium, was isolated from a sand sample collected in desert in the west of china. the strain comprised gram-positive, non-spore-forming, catalase-positive and irregular rods. comparative 16s rrna gene sequencing analysis demonstrated that the strain, gimn1.010(t), belonged to the genus corynebacterium and was closely related to c. glutamicum atcc 13032(t) (98.4%). however, dna relatedness between gimn1.010(t) and corynebacterium glutamicum was observed to be only 22.4±1.72% ... | 2011 | 21571935 |
| visualizing multi-omics data in metabolic networks with the software omix-a case study. | systems biology is a multi-disciplinary research field with the aim of understanding the function of complex processes in living organisms. these intracellular processes are described by biochemical networks. experimental studies in alliance with computer simulation lead to a continually increasing amount of data in liaison with different layers of biochemical networks. thus, visualization is very important for getting an overview of data in association with the network components. omix is a sof ... | 2011 | 21575673 |
| endolysin of bacteriophage bfk20: evidence of a catalytic and a cell wall binding domain. | a gene product of orf24' was identified on the genome of corynephage bfk20 as a putative phage endolysin. the protein of endolysin bfk20 (gp24') has a modular structure consisting of an n-terminal amidase_2 domain (gp24cd) and a c-terminal cell wall binding domain (gp24bd). the c-terminal domain is unrelated to any of the known cell wall binding domains of phage endolysins. the whole endolysin gene and the sequences of its n-terminal and c-terminal domains were cloned; proteins were expressed in ... | 2011 | 21592196 |
| rapid detection and molecular differentiation of toxigenic corynebacterium diphtheriae and corynebacterium ulcerans strains by lightcycler pcr. | the systemic symptoms of diphtheria are caused by the tox-encoded diphtheria toxin (dt) which is produced by toxigenic corynebacterium spp. besides the classical agent c. diphtheriae, the zoonotic pathogen c. ulcerans has increasingly been reported as an emerging pathogen for diphtheria. the reliable detection of toxigenic corynebacterium spp. is substantial for both diphtheria surveillance in the public health sector and the clinical work-up of a patient with diphtheria-like symptoms. since the ... | 2011 | 21593261 |
| diphtheria in the south of france, march 2011. | 2011 | 21596008 | |
| [influence of microbial-epithelial interactions on biological characteristics of vaginal microflora]. | evaluation of influence of microbial epithelial interactions on growth properties and antagonistic activity of dominant and associated vaginal microflora. | 2011 | 21598616 |
| [micro technologies in express diagnostics of toxigenic corynebacterium diphtheriae strains]. | development of micro technologies based approach for express diagnostics of toxigenic c. diphtheriae strains. | 2011 | 21598618 |
| airborne fungi and bacteria in indoor and outdoor environment of the pediatric unit of edirne government hospital. | this study was performed between january 2004 and december 2004 in 13 stations in the pediatric unit of edirne government hospital in order to determine the outdoor and indoor airborne microfungal and bacterial contents. the results of air samplings revealed that 1,376 microfungal and 2,429 bacterial colonies in total were isolated. the isolated microfungal specimens were identified and 65 species from 16 genera were determined. among these, the most frequent genus was cladosporium with 462 colo ... | 2011 | 21611848 |
| improved specificity of a multiplex immunoassay for the quantitation of anti-diphtheria toxin antibodies with the use of diphtheria toxoid. | a non-specific binding of antibodies to diphtheria toxin, especially in adult serum samples, was observed in our diphtheria-tetanus-pertussis multiplex immunoassay (dtap4 mia). this could be significantly reduced by the use of diphtheria toxoid, achieving a good correlation with the vero cell neutralization test and the toxin binding inhibition assay. | 2011 | 21613460 |
| osmotic stress response in c. glutamicum: impact of channel- and transporter-mediated potassium accumulation. | potassium accumulation is an essential aspect of bacterial response to diverse stress situations; consequently its uptake plays a pivotal role. here, we show that the gram-positive soil bacterium corynebacterium glutamicum which is employed for the large-scale industrial production of amino acids requires potassium under conditions of ionic and non-ionic osmotic stress. besides the accumulation of high concentrations of potassium contributing significantly to the osmotic potential of the cytopla ... | 2011 | 21614527 |
| rapid hybrid de novo assembly of a microbial genome using only short reads: corynebacterium pseudotuberculosis i19 as a case study. | due to the advent of the so-called next-generation sequencing (ngs) technologies the amount of monetary and temporal resources for whole-genome sequencing has been reduced by several orders of magnitude. sequence reads can be assembled either by anchoring them directly onto an available reference genome (classical reference assembly), or can be concatenated by overlap (de novo assembly). the latter strategy is preferable because it tends to maintain the architecture of the genome sequence the ho ... | 2011 | 21620904 |
| identification of spia that interacts with corynebacterium glutamicum whca using two-hybrid system. | the corynebacterium glutamicum whca gene is known to play a negative role in the expression of genes responding to oxidative stress. the encoded protein contains conserved cysteines, which likely coordinate the redox-sensitive fe-s cluster. to identify proteins which may interact with whca, we employed a two-hybrid system utilizing whca as a 'bait'. upon screening, several partner proteins were isolated from the c. glutamicum genomic library. sequencing analysis of the isolated clones revealed o ... | 2011 | 21623894 |
| strategies to prevent, treat, and provoke corynebacterium-associated hyperkeratosis in athymic nude mice. | athymic nude mice infected with corynebacterium bovis typically exhibit transient hyperkeratotic dermatitis. our vivarium experienced an increased incidence of disease characterized by persistent skin lesions and increased mortality, leading to this study. for detection of infection, skin and buccal swab methods showed comparable sensitivities in nude mice. various prevention, treatment, and eradication strategies were evaluated through clinical assessment, microbiology, and histopathology. in e ... | 2011 | 21640035 |