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aerobic and anaerobic microbial degradation of crude (4-methylcyclohexyl)methanol in river sediments.cyclohexane and some of its derivatives have been a major concern because of their significant adverse human health effects and widespread occurrence in the environment. the 2014 west virginia chemical spill has raised public attention to (4-methylcyclohexyl)methanol (4-mchm), one cyclohexane derivative, which is widely used in coal processing but largely ignored. in particular, the environmental fate of its primary components, cis- and trans-4-mchm, remains largely unexplored. this study aimed ...201626780132
catalytic, kinetic and thermodynamic properties of bacillus pumilus fh9 keratinase conjugated with activated pectin.bacillus pumilus fh9 keratinase was covalently coupled to several oxidized polysaccharides. the conjugates were evaluated for the retained activity, kinetic and thermodynamic stability. among all preparations, the conjugated enzyme with oxidized pectin had the highest recovered activity (71.75%) and the highest thermal stability at 60°c (t1/2=333 min). compared to the native enzyme, the conjugated preparation exhibited higher optimum temperature, lower activation energy (ea), lower deactivation ...201626743746
biosynthesis and characterization of a thermostable, alkali-tolerant chitinase from bacillus pumilus jubch08 displaying antagonism against phytopathogenic fusarium oxysporum.the present investigation highlights the process parameters influencing the submerged fermentation of chitinase by bacillus pumilus jubch08, purification and characterization of the enzyme and determination of antagonistic activity of the bacterium against fusarium oxysporum. medium supplemented with 0.5 % chitin and peptone, at initial ph 8.0, when incubated at 35 °c for 72 h favored highest chitinase production. the enzyme was purified 25.1-fold to homogeneity. the chitinase was found to be th ...201628330157
sensitive change of iso-branched fatty acid (iso-15:0) in bacillus pumilus pamc 23174 in response to environmental changes.in this study, the environmental adaptive metabolic processes were investigated using a psychrotrophic polar bacterium bacillus pumilus pamc 23174 in response to various temperatures and nutrients, especially in regard to the synthesis of fatty acids. fatty acid methyl ester analysis was performed using gas chromatography-mass spectrometry and we found that a sensitive changes in iso-branched fatty acid (iso-15:0) synthesis occurred when adjusting the nutritional ratio of branched chain fatty ac ...201626566954
chitin from penaeus merguiensis via microbial fermentation processing and antioxidant activity.the ability of three proteases producing bacteria such as pseudomonas aeruginosa, serratia marcescens, and bacillus pumilus on the demineralization and deproteinzation efficiency of shrimp waste, for chitin extraction, was investigated. statistical analysis of data was showed a significant difference between the percentage of demineralization and deproteinization in different bacteria species (p<0.05). the highest deproteinization (74.76%) and demineralization rate (78.46%) was obtained with p. ...201626519877
isolation and characterization of two novel psychrotrophic decabromodiphenyl ether-degrading bacteria from river sediments.decabromodiphenyl ether (bde-209) is a brominated flame retardant and a priority contaminant. currently, little information is available about its significance in the environment, specifically about its susceptibility to aerobic biotransformation at low temperature. in this work, five phylogenetically diverse bde-209-degrading bacterial strains were isolated from river sediments of northern china. these strains were distributed among four different genera-acinetobacter, pseudomonas, bacillus and ...201626517991
the role of metals in the reaction catalyzed by metal-ion-independent bacillary rnase.extracellular enzymes of intestinal microbiota are the key agents that affect functional activity of the body as they directly interact with epithelial and immune cells. several species of the bacillus genus, like bacillus pumilus, a common producer of extracellular rnase binase, can populate the intestinal microbiome as a colonizing organism. without involving metal ions as cofactors, binase depolymerizes rna by cleaving the 3',5'-phosphodiester bond and generates 2',3'-cyclic guanosine phospha ...201628096759
two-stage phyto-microremediation of tannery effluent by spirodela polyrrhiza (l.) schleid. and chromium resistant bacteria.two-stage sequential treatment of tannery effluent was conducted employing a wetland plant, spirodela polyrrhiza (l.) schleid., and chromium (cr) resistant bacterial strains. the bacterial strains were isolated from cr-enriched environmental matrices and rhizosphere of spirodela polyrrhiza. the phyto-rhizoremediation of tannery effluent by spirodela and its rhizospheric bacteria (cellulomonas biazotea apbr1-6, bacillus safensis apbr2-12, staphylococcus warneri apbr3-5, microbacterium oleivorans ...201627318662
development of an efficient bacterial consortium for the potential remediation of hydrocarbons from contaminated sites.the intrinsic biodegradability of hydrocarbons and the distribution of proficient degrading microorganisms in the environment are very crucial for the implementation of bioremediation practices. among others, one of the most favorable methods that can enhance the effectiveness of bioremediation of hydrocarbon-contaminated environment is the application of biosurfactant producing microbes. in the present study, the biodegradation capacities of native bacterial consortia toward total petroleum hyd ...201627471499
diversity of indigenous endophytic bacteria associated with the roots of chinese cabbage (brassica campestris l.) cultivars and their antagonism towards pathogens.the study aimed to reveal the diversity of endophytic bacteria in the roots of chinese cabbage (cc) cultivated in two areas in korea, namely, seosang-gun (ss) and haenam-gun (hn), and also in a transgenic plant (tp) from the laboratory. a total of 653 colonies were isolated from the interior of cc roots, comprising 118, 302, and 233 isolates from ss, hn, and tp samples, respectively. based on 16s rrna gene sequence analysis, the isolates belonged to four major phylogenetic groups: high-g+c gram- ...201627095454
isolation and characterization of bacterial endophytes of curcuma longa l.fourteen endophytic bacterial isolates were isolated from the rhizome of curcuma longa l. were characterized on the basis of morphology, biochemical characteristics and 16s rrna gene sequence analysis. the isolates were identified to six strains namely bacillus cereus (ecl1), bacillus thuringiensis (ecl2), bacillus sp. (ecl3), bacillus pumilis (ecl4), pseudomonas putida (ecl5), and clavibacter michiganensis (ecl6). all the strains produced iaa and solubilized phosphate and only two strains produ ...201628330130
effect of oral administration of bacillus coagulans b37 and bacillus pumilus b9 strains on fecal coliforms, lactobacillus and bacillus spp. in rat animal model.to investigate the effect of oral administration of two bacillus strains on fecal coliforms, lactobacillus and bacillus spp. in rat animal model.201627536040
a phosphate starvation-inducible ribonuclease of bacillus licheniformis.the bli03719 protein of bacillus licheniformis dsm13 belongs to the most abundant extracellular proteins under phosphate starvation conditions. in this study, the function of this phosphate starvation inducible protein was determined. an amino-acid sequence analysis of the bli03719-encoding gene showed a high similarity with genes encoding the barnase of bacillus amyloliquefaciens fzb42 and binase-like rnase of bacillus pumilus sarf-032. the comparison of the control strain and a bli03719-defici ...201627160576
plantazolicin is an ultra-narrow spectrum antibiotic that targets the bacillus anthracis membrane.plantazolicin (pzn) is a ribosomally synthesized and post-translationally modified natural product from bacillus methylotrophicus fzb42 and bacillus pumilus. extensive tailoring to twelve of the fourteen amino acid residues in the mature natural product endows pzn with not only a rigid, polyheterocyclic structure, but also antibacterial activity. here we report a remarkably discriminatory activity of pzn toward bacillus anthracis, which rivals a previously-described gamma (γ) phage lysis assay i ...201627152321
identification and pathogenic potential of clinical bacillus and paenibacillus isolates.the soil-related bacillus and paenibacillus species have increasingly been implicated in various human diseases. nevertheless, their identification still poses problems in the clinical microbiology laboratory and, with the exception of bacillus anthracis and bacillus cereus, little is known on their pathogenicity for humans. in this study, we evaluated the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry (maldi-tof ms) in the identification of clinical isolates ...201627031639
genome sequence of bacillus pumilus strain bonn, isolated from an anthrax-like necrotic skin infection site of a child.we report the draft genome sequence of bacillus pumilus strain bonn associated with human skin infection. b. pumilus bonn was isolated from a carbuncle-like necrotic site, resembling cutaneous anthrax, on the back of the hand of a 10-year-old child.201626868410
bacterial biota of nigeen lake waters (kashmir valley).one of the greatest apprehensions of water consumers all over the world with respect to the quality of drinking water is its contamination with pathogenic microorganisms. this research work determined the potential bacterial contaminants of the waters of nigeen lake, a subsidiary of dal lake and is regarded as a separate lake in kashmir. the study was carried out from may 2014 to november 2014 excluding august and september at four different sites. during the study the bacterial flora showed var ...201627165539
utilization of microbial community potential for removal of chlorpyrifos: a review.chlorpyrifos (cp) is the most commonly used pesticide in agricultural fields worldwide. exposure to cp and its metabolites creates severe neuron-disorders in human beings. improper handling and uncontrolled application of cp by farmers have lead to the contamination of surface and ground water bodies. biodegradation offers an efficient and cost effective method for the removal of cp and other toxic organophosphorus pesticides from the contaminated environment. the degradation of cp by various mi ...201625782532
optimization of fermentation media and growth conditions for microbial xylanase production.efficiency of cellulase-free xylanases is one of the determining factors in paper and pulp industries. use of microbes which can produce cellulase-free xylanases may help to overcome the current challenges in kraft pulp processing. isolation and screening of microorganisms from local samples offers a possibility for obtaining the potential microbes for this purpose. this research was therefore aimed to collect, screen, characterize and identify potential cellulase-free xylanase producers. a tota ...201628330199
pathogenic features of heterotrophic plate count bacteria from drinking-water boreholes.evidence suggests that heterotrophic plate count (hpc) bacteria may be hazardous to humans with weakened health. we investigated the pathogenic potential of hpc bacteria from untreated borehole water, consumed by humans, for: their haemolytic properties, the production of extracellular enzymes such as dnase, proteinase, lipase, lecithinase, hyaluronidase and chondroitinase, the effect simulated gastric fluid has on their survival, as well as the bacteria's antibiotic-susceptible profile. hutu-80 ...201627959868
analysis of bacterial communities associated with potting media.potting media are commonly used by growers in different parts of the world for potted plants, raising seedlings and for improving soil characteristics. this study was conducted to characterize bacterial communities occurring in 13 commercial potting media products originating from seven countries.201626844021
bacillus pumilus septic arthritis in a healthy child.we report a case of septic arthritis caused by a bacillus species, b. pumilus, occurring in a healthy child. this organism rarely causes serious infections and has only been described in newborns and immunocompromised individuals or as a skin infection. this child developed an indolent joint swelling after a minor skin injury, and symptoms were initially thought most consistent with chronic arthritis. the case demonstrates that clinicians should consider joint infection in children presenting wi ...201627366165
effect of metal ions and petrochemicals on bioremediation of chlorpyrifos in aerobic sequencing batch bioreactor (asbr).application of chlorpyrifos (cp) has increased its environmental concentration. increasing cp concentration has increased chances of adverse health effects. its removal from environment has attained researcher's attention. cp degrading bacterial strains were isolated from wastewater and agricultural soil. finally, selected five bacterial strains were identified using 16s rrna nucleotide sequence analysis as pseudomonas kilonensis srk1, serratia marcescens srk2, bacillus pumilus srk4, achromobact ...201627470246
antimicrobial activities of bacteria associated with the brown alga padina pavonica.macroalgae belonging to the genus padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. here we report antibacterial activities of epiphytic bacteria isolated from padina pavonica (peacocks tail) located on northern coast of tunisia. eighteen isolates were obtained in pure culture and tested ...201627462308
plant growth-promoting rhizobacteria inoculation to enhance vegetative growth, nitrogen fixation and nitrogen remobilisation of maize under greenhouse conditions.plant growth-promoting rhizobacteria (pgpr) may provide a biological alternative to fix atmospheric n2 and delay n remobilisation in maize plant to increase crop yield, based on an understanding that plant-n remobilisation is directly correlated to its plant senescence. thus, four pgpr strains were selected from a series of bacterial strains isolated from maize roots at two locations in malaysia. the pgpr strains were screened in vitro for their biochemical plant growth-promoting (pgp) abilities ...201627011317
anthracnose of lucky bamboo dracaena sanderiana caused by the fungus colletotrichum dracaenophilum in egypt.dracaena sanderiana, of the family liliaceae, is among the ornamental plants most frequently imported into egypt. typical anthracnose symptoms were observed on the stems of imported d. sanderiana samples. the pathogen was isolated, demonstrated to be pathogenic based on koch's rule and identified as colletotrichum dracaenophilum. the optimum temperature for its growth ranges from 25 to 30 °c, maintained for 8 days. kemazed 50% wettable powder (wp) was the most effective fungicide against the pat ...201627222738
bacillus pumilus cyanide dihydratase mutants with higher catalytic activity.cyanide degrading nitrilases are noted for their potential to detoxify industrial wastewater contaminated with cyanide. however, such application would benefit from an improvement to characteristics such as their catalytic activity and stability. following error-prone pcr for random mutagenesis, several cyanide dihydratase mutants from bacillus pumilus were isolated based on improved catalysis. four point mutations, k93r, d172n, a202t, and e327k were characterized and their effects on kinetics, ...201627570524
probing an interfacial surface in the cyanide dihydratase from bacillus pumilus, a spiral forming nitrilase.nitrilases are of significant interest both due to their potential for industrial production of valuable products as well as degradation of hazardous nitrile-containing wastes. all known functional members of the nitrilase superfamily have an underlying dimer structure. the true nitrilases expand upon this basic dimer and form large spiral or helical homo-oligomers. the formation of this larger structure is linked to both the activity and substrate specificity of these nitrilases. the sequences ...201526779137
aerobic cyanide degradation by bacterial isolates from cassava factory wastewater.ten bacterial strains that utilize cyanide (cn) as a nitrogen source were isolated from cassava factory wastewater after enrichment in a liquid media containing sodium cyanide (1 mm) and glucose (0.2% w/v). the strains could tolerate and grow in cyanide concentrations of up to 5 mm. increased cyanide levels in the media caused an extension of lag phase in the bacterial growth indicating that they need some period of acclimatisation. the rate of cyanide removal by the strains depends on the initi ...201526413045
biochemical properties and yields of diverse bacterial laccase-like multicopper oxidases expressed in escherichia coli.laccases are multi-copper oxidases that oxidize a broad range of substrates at the expense of molecular oxygen, without any need for co-factor regeneration. these enzymes bear high potential for the sustainable synthesis of fine chemicals and the modification of (bio)polymers. here we describe cloning and expression of five novel bacterial laccase-like multi copper oxidases (lmcos) of diverse origin which were identified by homology searches in online databases. activity yields under different e ...201526068013
structural characterization and antimicrobial activity of a biosurfactant obtained from bacillus pumilus dsvp18 grown on potato peels.biosurfactants constitute a structurally diverse group of surface-active compounds derived from microorganisms. they are widely used industrially in various industrial applications such as pharmaceutical and environmental sectors. major limiting factor in biosurfactant production is their production cost.201526495106
synthesis and characterization of bioactive acylpyrazolone sulfanilamides and their transition metal complexes: single crystal structure of 4-benzoyl-3-methyl-1-phenyl-2-pyrazolin-5-one sulfanilamide.two schiff base ligands ampp-sn 1 and bmpp-sn 2, afforded by a condensation reaction between sulfanilamide and the respective acylpyrazolone carbonyl precursors, their mn(ii), co(ii), ni(ii), and cu(ii) complexes prepared by the reaction of ligands and corresponding metal salts in aqueous solutions, were synthesized and then characterized by both analytical and spectroscopic methods, in a view to developing new improved bioactive materials with novel properties. on the basis of elemental analysi ...201526106285
isolation of potential probiotic bacillus spp. and assessment of their subcellular components to induce immune responses in labeo rohita against aeromonas hydrophila.bacillus species isolated from the gut of healthy labeo rohita (hamilton) were screened for antibacterial activity against selected fish pathogens. among the isolates, kadr5 and kadr6 showed antibacterial activity, tolerated low ph and high bile concentrations and were susceptibility to various antibiotics. based on morphological and biochemical tests and 16s rrna gene analysis the probiotic strains kadr5 and kadr6 were identified as bacillus licheniformis and bacillus pumilus, respectively. the ...201525917974
influence of rhizobacterial volatiles on the root system architecture and the production and allocation of biomass in the model grass brachypodium distachyon (l.) p. beauv.plant growth-promoting rhizobacteria are increasingly being seen as a way of complementing conventional inputs in agricultural systems. the effects on their host plants are diverse and include volatile-mediated growth enhancement. this study sought to assess the effects of bacterial volatiles on the biomass production and root system architecture of the model grass brachypodium distachyon (l.) beauv.201526264238
[inhibition of bacillus pumilus ar03 on alternaria alternata and erysiphe cichoracearum on tobacco].this study aimed to evaluate the efficiency of bacillus pumilus ar03 against altenaria alternata and erysiphe cichoracearum. the antagonistic activities were studied in the way of co-culture on plate, inhibition of pathogen conidia germination on concave slides and lb agar medium. in the investigation, the water suspension of living cells of strain ar03, at 3 x 10(8) cfu . ml-1 had a remarkable inhibition effect on hyphae growth and conidia germination of a. alternata and caused hyphae deformati ...201526995927
assessment of microbial contamination within working environments of different types of composting plants.the objective of the study was to determine the degree of microbiological contamination, type of microflora, bioaerosol particle size distribution, and concentration of endotoxins in dust in different types of composting plants. in addition, this study provides a list of indicator microorganisms that pose a biological threat in composting facilities, based on their prevalence within the workplace, source of isolation, and health hazards. we undertook microbiological analysis of the air, work sur ...201525947216
utilization of potato starch processing wastes to produce animal feed with high lysine content.this work aims to utilize wastes from the potato starch industry to produce single-cell protein (scp) with high lysine content as animal feed. in this work, s-(2-aminoethyl)-l-cysteine hydrochloride-resistant bacillus pumilus e1 was used to produce scp with high lysine content, whereas aspergillus niger was used to degrade cellulose biomass and candida utilis was used to improve the smell and palatability of the feed. an orthogonal design was used to optimize the process of fermentation for maxi ...201525189407
detection of boron removal capacities of different microorganisms in wastewater and effective removal process.in this study boron removal capacities of different microorganisms were tested. candida tropicalis, rhodotorula mucilaginosa, micrococcus luteus, bacillus thuringiensis, bacillus cereus, bacillus megaterium, bacillus pumilus, pseudomonas aeruginosa and aspergillus versicolor were examined for their boron bioaccumulation capacities in simulated municipal wastewater. a. versicolor and b. cereus were found as the most boron-tolerant microorganisms in the experiments. also boron bioaccumulation yiel ...201526540546
chitin extraction from crab shells by bacillus bacteria. biological activities of fermented crab supernatants.crab shells waste were fermented using six protease-producing bacillus species (bacillus subtilis a26, bacillus mojavensis a21, bacillus pumilus a1, bacillus amyloliquefaciens an6, bacillus licheniformis nh1 and bacillus cereus bg1) for the production of chitin and fermented-crab supernatants (fcss). in medium containing only crab shells, the highest demineralization dm was obtained with b. licheniformis nh1 (83±0.5%) and b. pumilus a1 (80±0.6%), while the highest deproteinization (dp) was achie ...201525910648
characterization of deep sea fish gut bacteria with antagonistic potential, from centroscyllium fabricii (deep sea shark).the bacterial isolates from centroscyllium fabricii (deep sea shark) gut were screened for antagonistic activity by cross-streak method and disc diffusion assay. this study focuses on strain btss-3, which showed antimicrobial activity against pathogenic bacteria including salmonella typhimurium, proteus vulgaris, clostridium perfringens, staphylococcus aureus, bacillus cereus, bacillus circulans, bacillus macerans and bacillus pumilus. btss3 was subjected to phenotypic characterization using bio ...201525740801
identification of single nucleotide polymorphisms (snps) in the 16s rrna gene of foodborne bacillus spp.the main goal of this work was the identification of single nucleotide polymorphisms (snps) in the 16s rrna gene of foodborne bacillus spp. that may be useful for typing purposes. these species include, among others, bacillus cereus, an important pathogenic species involved in food poisoning, and bacillus licheniformis, bacillus subtilis and bacillus pumilus, which are causative agents of food spoilage described as responsible for foodborne disease outbreaks. with this purpose in mind, 52 bacill ...201525475292
bacillus zhangzhouensis sp. nov. and bacillus australimaris sp. nov.two strains, designated dw5-4t and nh7i_1t, were isolated from aquaculture water of shrimp farm of zhangzhou city in china and surface sediment of the south china sea, respectively. cells of the strains were gram-stain-positive, oxidase- and catalase-positive, rod-shaped and motile. the phylogenetic analyses based on 16s rrna gene sequences indicated that two strains belonged to the bacillus pumilus clade. the similarities of 16s rrna gene sequences between two strains dw5-4t and nh7i_1t was 99. ...201526705002
identification of bacillus strains by maldi tof ms using geometric approach.microorganism identification by maldi tof mass-spectrometry is based on the comparison of the mass spectrum of the studied organism with those of reference strains. it is a rapid and reliable method. however, commercial databases and programs are mostly designed for identification of clinically important strains and can be used only for particular mass spectrometer models. the need for open platforms and reference databases is obvious. in this study we describe a geometric approach for microorga ...201526592761
reclassification of bacillus invictae as a later heterotypic synonym of bacillus altitudinis.the aim of this study was to reclarify the taxonomic status of strain bacillus invictae bi.ffup1t by performing comparative analyses with the other four type strains within the bacillus pumilus group. the digital dna-dna hybridization (dddh) and average nucleotide identity (ani) values between strains b. invictae bi.ffup1t ( = dsmz 26896t = mccc 1a07089t), b. altitudinis 41kf2bt ( = dsmz 21631t = mccc 1a06452t), b. safensis fo-36bt ( = dsmz 19292t = mccc 1a6451t), b. pumilus atcc 7061t ( = dsmz ...201525977285
the status of the species bacillus aerophilus and bacillus stratosphericus. request for an opinion.during a study assessing the diversity of the bacillus pumilus group it became apparent that the type strains of both bacillus aerophilus and bacillus stratosphericus were not available from any established culture collection, nor from the authors who originally described them. therefore, type strains of these species cannot be included in any further scientific studies. it is therefore proposed that the judicial commission of the international committee of systematics of prokaryotes place the n ...201525479956
bacillus spp. from rainforest soil promote plant growth under limited nitrogen conditions.the aim of this study was to evaluate effects of pgpr (plant growth-promoting rhizobacteria) isolated from rainforest soil on different plants under limited nitrogen conditions.201525494882
genetic discrimination of foodborne pathogenic and spoilage bacillus spp. based on three housekeeping genes.bacillus genus includes foodborne pathogenic and spoilage-associated species, such as bacillus cereus, bacillus licheniformis, bacillus subtilis and bacillus pumilus. bacillus is also a heterogeneous genus that includes closely related species that are difficult to discriminate among, especially when well-conserved genes such as 16s rrna and 23s rrna are considered. the main goal of the present work was to study the usefulness of three housekeeping genes, the tu elongation factor (tuf), the dna ...201525475298
silver nanoparticles synthesis mediated by new isolates of bacillus spp., nanoparticle characterization and their activity against bean yellow mosaic virus and human pathogens.extracellular agents produced by newly isolated bacterial strains were able to catalyze the synthesis of silver nanoparticles (agnps). the most effective isolates were identified as bacillus pumilus, b. persicus, and bacillus licheniformis using molecular identification. dls analysis revealed that the agnps synthesized by the above strains were in the size range of 77-92 nm. tem observations showed that the nanoparticles were coated with a capping agent, which was probably involved in nanopartic ...201526029190
isolation of haemolytic bacilli from field-collected culicoides oxystoma and culicoides peregrinus: potential vectors of bluetongue virus in west bengal, india.two haemolytic bacterial strains of bacillus pumilus (cu1a, cu1b) and one blood-utilizing strain of bacillus licheniformis (cu2b) were isolated from relatively low numbers of field-collected females of culicoides oxystoma and culicoides peregrinus (diptera: ceratopogonidae). a total of 36 females, including 18 of each of c. oxystoma and c. peregrinus (consisting of one and a pool of eight blood-engorged specimens, and one and a pool of eight non-engorged specimens for each species), were tested. ...201525644315
bacillus species isolated from tungrymbai and bekang, naturally fermented soybean foods of india.tungrymbai and bekang are naturally fermented soybean foods commonly consumed in meghalaya and mizoram states of india. a total of 39 samples of tungrymbai and 43 samples of bekang were collected from different villages and markets of meghalaya and mizoram, respectively and were analysed for microbial load. in both tungrymbai and bekang, the average population of bacillus spp. was 8.2±0.1 log cfu/g. a total of 428 isolates of bacillus were isolated from tungrymbai (211) and bekang (217) for deta ...201525574846
isolation and molecular characterization of multidrug-resistant halophilic bacteria from shrimp farm effluents of parangipettai coastal waters.multidrug resistance of heterotrophic bacteria isolated from an aquaculture farm effluent in parangipettai, at the southeastern coast of india, was investigated. in the initial screening, 27 antibiotic-resistant strains were isolated. all the strains were tested for antibiotic susceptibility against chloramphenicol with varying concentrations. from these, two highly resistant strains, i.e. s1 and s5, were isolated. the selected strains were identified by 16s ribosomal rna (rrna) sequencing techn ...201525850744
coexpression of lactobacillus brevis adh with gdh or g6pdh in arxula adeninivorans for the synthesis of 1-(r)-phenylethanol.the yeast arxula adeninivorans was used for the overexpression of an adh gene of lactobacillus brevis coding for (r)-specific alcohol dehydrogenase (lbadh) to synthesise enantiomerically pure 1-(r)-phenylethanol. glucose dehydrogenase gene from bacillus megaterium (bmgdh) or glucose 6-phosphate dehydrogenase of bacillus pumilus (bpg6pdh) were coexpressed in arxula to regenerate the cofactor nadph by oxidising glucose or glucose 6-phosphate. the yeast strain expressing lbadh and bpg6pdh produced ...201525535093
matrix production, pigment synthesis, and sporulation in a marine isolated strain of bacillus pumilus.the ability to produce an extracellular matrix and form multicellular communities is an adaptive behavior shared by many bacteria. in bacillus subtilis, the model system for spore-forming bacteria, matrix production is one of the possible differentiation pathways that a cell can follow when vegetative growth is no longer feasible. while in b. subtilis the genetic system controlling matrix production has been studied in detail, it is still unclear whether other spore formers utilize similar mecha ...201526506360
studies on properties of the xylan‑binding domain and linker sequence of xylanase xyng1‑1 from paenibacillus campinasensis g1‑1.xylanase xyng1-1 from paenibacillus campinasensis g1-1 consists of a catalytic domain (cd), a family 6_36 carbohydrate-binding module which is a xylan-binding domain (xbd), and a linker sequence (ls)between them. the structure of xyng1-3 from bacillus pumilus g1-3 consists only of a cd. to investigate the functions and properties of the xbd and ls of xyng1-1, two truncated forms (xyng1-1cdl, xyng1-1cd) and three fusion derivatives (xyng1-3cdl, xyng1-3cdx and xyng1-3cdlx) were constructed and bio ...201526467249
sterilization resistance of bacterial spores explained with water chemistry.bacterial spores can survive for long periods without nutrients and in harsh environmental conditions. this survival is influenced by the structure of the spore, the presence of protective compounds, and water retention. these compounds, and the physical state of water in particular, allow some species of bacterial spores to survive sterilization schemes with hydrogen peroxide and uv light. the chemical nature of the spore core and its water has been a subject of some contention and the chemical ...201526435315
identification, sequencing and comparative analysis of pbp15.s plasmid from the newly described entomopathogen bacillus pumilus 15.1.the bacillus pumilus 15.1 strain, a recently described entomopathogenic strain active against ceratitis capitata, contains at least two extrachromosomal elements, pbp15.1s and pbp15.1b. given that b. pumilus is not a typical entomopathogenic bacterium, the acquisition of this extrachromosomal dna may explain why b. pumilus 15.1 is toxic to an insect. one of the plasmids present in the strain, the pbp15.1s plasmid, was sub-cloned, sequenced and analyzed using bioinformatics to identify any potent ...201526416357
antibacterial metabolites and bacteriolytic enzymes produced by bacillus pumilus during bacteriolysis of arthrobacter citreus.the marine isolate bacillus pumilus sbug 1800 is able to lyse living cells of arthrobacter citreus on solid media as well as pasteurized a. citreus cells in liquid mineral salt medium. the cultivation of b. pumilus in the presence of pasteurized a. citreus is accompanied by an enhanced production of 2,5-diketopiperazines (dkps). dkps inhibit bacterial growth, but do not seem to cause bacteriolysis. this study shows that b. pumilus also lyses living cells of a. citreus in co-culture experiments a ...201525678259
unprecedented access of phenolic substrates to the heme active site of a catalase: substrate binding and peroxidase-like reactivity of bacillus pumilus catalase monitored by x-ray crystallography and epr spectroscopy.heme-containing catalases and catalase-peroxidases catalyze the dismutation of hydrogen peroxide as their predominant catalytic activity, but in addition, individual enzymes support low levels of peroxidase and oxidase activities, produce superoxide, and activate isoniazid as an antitubercular drug. the recent report of a heme enzyme with catalase, peroxidase and penicillin oxidase activities in bacillus pumilus and its categorization as an unusual catalase-peroxidase led us to investigate the e ...201525663126
complete genome sequence of bacillus pumilus strain wp8, an efficient plant growth-promoting rhizobacterium.bacillus pumilus strain wp8 is an efficient plant growth-promoting rhizobacterium. here, we present the complete genome of wp8 and its genes involved in plant growth promotion and biocontrol.201525614565
c-terminal hybrid mutant of bacillus pumilus cyanide dihydratase dramatically enhances thermal stability and ph tolerance by reinforcing oligomerization.to investigate the impact of the highly variable c-terminal domain of cyanide dihydratase, a member of the nitrilase superfamily, on its activity and stability.201525597384
genomic and chemical insights into biosurfactant production by the mangrove-derived strain bacillus safensis ccma-560.many bacillus species can produce biosurfactant, although most of the studies on lipopeptide production by this genus have been focused on bacillus subtilis. surfactants are broadly used in pharmaceutical, food and petroleum industry, and biological surfactant shows some advantages over the chemical surfactants, such as less toxicity, production from renewable, cheaper feedstocks and development of novel recombinant hyperproducer strains. this study is aimed to unveil the biosurfactant metabolic ...201525586584
increased mrna expression of selected pro-inflammatory factors in inflamed bovine endometrium in vivo as well as in endometrial epithelial cells exposed to bacillus pumilus in vitro.endometrial epithelium plays a crucial role in the first immune response to invading bacteria by producing cytokines and chemokines. the aim of this study was to investigate the first inflammatory response of the endometrium in vivo and in vitro. gene expression of several pro-inflammatory factors and toll-like receptors (tlr2, -4, -6) was determined in endometrial cytobrush samples obtained from healthy cows and cows with clinical or subclinical endometritis. endometrial epithelial cells were c ...201525562589
heterologous expression of mannanase and developing a new reporter gene system in lactobacillus casei and escherichia coli.reporter gene systems are useful for studying bacterial molecular biology, including the regulation of gene expression and the histochemical analysis of protein products. here, two genes, β-1,4-mannanase (manb) from bacillus pumilus and β-glucuronidase (gusa) from escherichia coli k12, were cloned into the expression vector pelx1. the expression patterns of these reporter genes in lactobacillus casei were investigated by measuring their enzymatic activities and estimating their recombinant prote ...201526562012
draft genome sequence of the entomopathogenic bacterium bacillus pumilus 15.1, a strain highly toxic to the mediterranean fruit fly ceratitis capitata.we present the draft whole-genome sequence of the entomopathogenic bacillus pumilus 15.1 strain that consists of 3,795,691 bp and 3,776 predicted protein-coding genes. this genome sequence provides the basis for understanding the potential mechanism behind the toxicity and virulence of b. pumilus 15.1 against the mediterranean fruit fly.201526404596
construction of novel shuttle expression vectors for gene expression in bacillus subtilis and bacillus pumilus.a native plasmid (psu01) was detected by genome sequencing of bacillus subtilis strain s1-4. two psu01-based shuttle expression vectors psu02-ap and psu03-ap were constructed enabling stable replication in b. subtilis wb600. these vectors contained the reporter gene apre, encoding an alkaline protease from bacillus pumilus ba06. the expression vector psu03-ap only possessed the minimal replication elements (rep, sso, dso) and exhibited more stability on structure, suggesting that the rest of the ...201526377132
sugarcane bagasse as support for immobilization of bacillus pumilus hz-2 and its use in bioremediation of mesotrione-contaminated soils.the degrading microorganisms isolated from environment usually fail to degrade pollutants when used for bioremediation of contaminated soils; thus, additional treatments are needed to enhance biodegradation. in the present study, the potential of sugarcane bagasse as bacteria-immobilizing support was investigated in mesotrione biodegradation. a novel isolate bacillus pumilus hz-2 was applied in bacterial immobilization, which was capable of degrading over 95 % of mesotrione at initial concentrat ...201526337896
draft genome sequence of the shellfish larval probiotic bacillus pumilus ri06-95.bacillus pumilus ri06-95 is a marine bacterium isolated in narragansett, rhode island, which has shown probiotic activity against marine pathogens in larval shellfish. we report the genome of b. pumilus ri06-95, which provides insight into the microbe's probiotic ability and may be used in future studies of the probiotic mechanism.201526337873
identification of oxidoreductases from the petroleum bacillus safensis strain.a gram-positive bacterium, denominated cfa-06, was isolated from brazilian petroleum in the campos basin and is responsible for the degradation of aromatic compounds and petroleum aromatic fractions. the cfa-06 strain was identified as bacillus safensis using the 16s rrna and gyrase b sequence. enzymatic assays revealed the presence of two oxidoreductases: a catalase and a new oxidoreductase. the oxidoreductases were enzymatically digested and analyzed via esi-ltq-orbitrap mass spectrometry. the ...201528352585
cyanide hydratases and cyanide dihydratases: emerging tools in the biodegradation and biodetection of cyanide.the purpose of this study is to summarize the current knowledge of the enzymes which are involved in the hydrolysis of cyanide, i.e., cyanide hydratases (chts; ec 4.2.1.66) and cyanide dihydratases (cynd; ec 3.5.5.1). chts are probably exclusively produced by filamentous fungi and widely occur in these organisms; in contrast, cynds were only found in a few bacterial genera. chts differ from cynds in their reaction products (formamide vs. formic acid and ammonia, respectively). several chts were ...201526329848
microbial selenium nanoparticles (senps) and their application as a sensitive hydrogen peroxide biosensor.the cell-free extract, a crude enzyme (cytosolic and membrane fraction) obtained from an environmental isolate, bacillus pumilus sp. bab-3706 worked as excellent in reducing as well as stabilizing agent and facilitated the formation of stable colloidal selenium nanoparticles (senps). resulting nanoparticles were characterized using uv-vis spectrophotometer, tem, edax, ft-ir and xrd, respectively. a working electrode was modified by coating the surface of indium tin oxide (ito) with colloidal sen ...201526319569
a novel thermostable gh5_7 β-mannanase from bacillus pumilus gbsw19 and its application in manno-oligosaccharides (mos) production.a novel thermostable mannanase from a newly isolated bacillus pumilus gbsw19 has been identified, expressed, purified and characterized. the enzyme shows a structure comprising a 28 amino acid signal peptide, a glycoside hydrolase family 5 (gh5) catalytic domain and no carbohydrate-binding module. the recombinant mannanase has molecular weight of 45 kda with an optimal ph around 6.5 and is stable in the range from ph 5-11. meanwhile, the optimal temperature is around 65 °c, and it retains 50% re ...201526215338
effects of direct-fed bacillus pumilus 8g-134 on feed intake, milk yield, milk composition, feed conversion, and health condition of pre- and postpartum holstein cows.the usage of direct-fed microbials (dfm) has become common in the dairy industry, but questions regarding choice of strain, mode of action, and efficacy remain prevalent. the objective of this study was to evaluate the effects of a dfm (bacillus pumilus 8g-134) on pre- and postpartum performance and incidence of subclinical ketosis in early lactation. forty-three multiparous holstein cows were assigned to 2 treatments in a randomized complete block design; cows in the direct-fed microbial treatm ...201526162791
production of a cellulase-free alkaline xylanase from bacillus pumilus mtcc 5015 by submerged fermentation and its application in biobleaching.here, we described the production of a cellulase-free alkaline xylanase from bacillus pumilus mtcc 5015 by submerged fermentation and its application in biobleaching. various process parameters affecting xylanase production by b. pumilus were optimized by adopting a plackett-burman design (pbd) as well as response surface methodology (rsm). these statistical methods aid in improving the enzyme yield by analysing the individual crucial components of the medium. maximum production was obtained wit ...201526155675
synthesis and characterization of magnetite/silver/antibiotic nanocomposites for targeted antimicrobial therapy.the article is devoted to preparation and characterization of magnetite/silver/antibiotic nanocomposites for targeted antimicrobial therapy. magnetite nanopowder was produced by thermochemical technique; silver was deposited on the magnetite nanoparticles in the form of silver clusters. magnetite/silver nanocomposite was investigated by xrd, sem, tem, afm, xps, edx techniques. adsorptivity of magnetite/silver nanocomposite towards seven antibiotics from five different groups was investigated. it ...201526117765
[identification of 2',3'-cgmp as an intermediate of rna catalytic cleavage by binase and evaluation of its biological action].binase--bacillus pumilus rnase--endonuclease cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-oh residue of adjacent nucleotides with the formation of corresponding intermediate 2',3'-cgmp. subsequent hydrolysis of 2',3'-cgmp into 3'-phosphate is highly specific and occurs slowly so the question arises about the existing time of that positional isomer during rna catalytic cleavage by binase and about 2',3'-cgmp role in antitumor activity of the enzyme: in present work b ...201526050470
genomic analysis of a ginger pathogen bacillus pumilus providing the understanding to the pathogenesis and the novel control strategy.bacillus pumilus has been widely identified as a pathogen of plant and human, while the genetic information is rarely available for pathogenic b. pumilus strains. b. pumilus gr8 is a pathogen that causes ginger rhizome rot disease by invading ginger rhizome parenchymatous tissues, growing in the extracellular space, and producing plant cell wall-degrading enzymes to destroy ginger cells. in this study, the genome of gr8 was sequenced and characterized. this genome was the third completely sequen ...201525989507
salt stress represses production of extracellular proteases in bacillus pumilus.bacillus pumilus is able to secrete subtilisin-like prote-ases, one of which has been purified and characterized biochemically, demonstrating great potential for use in industrial applications. in the current study, the biosynthesis and transcription of extracellular pro-teases in b. pumilus (ba06) under salt stress were investigated using various methods, including a proteolytic assay, zymogram analysis, and real-time pcr. our results showed that total extracellular proteolytic activity, both i ...201525966269
complete genome sequence of bacillus pumilus w3: a strain exhibiting high laccase activity.here we report the full genome sequence of bacillus pumilus w3, which was isolated from raw gallnut honey in nandan county, guangxi province of china, showing high cota-laccase activity. the w3 strain contains 3,745,123bp with gc content of 41.39%, and contains 3695 protein-coding genes, 21 rrnas and 70 trnas.201525957807
sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide.bacillus pumilus safr-032 spores isolated from a clean room environment are known to exhibit enhanced resistance to peroxide, desiccation, uv radiation and chemical disinfection than other spore-forming bacteria. the survival of b. pumilus safr-032 spores to standard clean room sterilization practices requires development of more stringent disinfection agents. here, we report the effects of a stabilized chlorine dioxide-based biocidal agent against spores of b. pumilus safr-032 and bacillus subt ...201525897406
[expression optimization of bacillus pumilus subtilisin-like proteinase].the characteristics of bacillus pumilus subtilisin-like protease expression were investigated. potential binding sites for the transcription factor degu~p have been identified in the regulatory region of the enzyme gene, which have been optimized for interaction with the regulatory protein. the expression of the extracellular subtilisin-like protease modified gene has been examined. it was established that the modification of one of the sites has resulted in increased expression of the proteinas ...201525895372
efficient secretory production of cota-laccase and its application in the decolorization and detoxification of industrial textile wastewater.fungal laccases are typically unstable at high ph and temperature conditions, which limit their application in the decolorization of textile wastewater. by contrast, the highly stable bacterial laccases can function within a wider ph range and at high temperatures, thus have significant potential in treatment for textile wastewater. in our previous work, a thermo-alkali-stable cota-laccase gene was cloned from bacillus pumilus w3 and overexpressed in escherichia coli. in this study, the robust c ...201525847445
growth and (137)cs uptake of four brassica species influenced by inoculation with a plant growth-promoting rhizobacterium bacillus pumilus in three contaminated farmlands in fukushima prefecture, japan.the effectiveness of the plant growth-promoting rhizobacterium bacillus pumilus regarding growth promotion and radiocesium ((137)cs) uptake was evaluated in four brassica species grown on different (137)cs contaminated farmlands at fukushima prefecture in japan from june to august 2012. b. pumilus inoculation did not enhance growth in any of the plants, although it resulted in a significant increase of (137)cs concentration and higher (137)cs transfer from the soil to plants. the brassica specie ...201525847170
a membraneless air-breathing hydrogen biofuel cell based on direct wiring of thermostable enzymes on carbon nanotube electrodes.a biocathode was designed by the modification of a carbon nanotube (cnt) gas-diffusion electrode with bilirubin oxidase from bacillus pumilus, achieving high current densities up to 3 ma cm(-2) for the reduction of o2 from air. a membraneless air-breathing hydrogen biofuel cell was designed by combination of this cathode with a functionalized cnt-based hydrogenase anode.201525845356
the hyperaccumulator sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil.endophyte-assisted phytoremediation has recently been suggested as a successful approach for ecological restoration of metal contaminated soils, however little information is available on the influence of endophytic bacteria on the phytoextraction capacity of metal hyperaccumulating plants in multi-metal polluted soils. the aims of our study were to isolate and characterize metal-resistant and 1-aminocyclopropane-1-carboxylate (acc) utilizing endophytic bacteria from tissues of the newly discove ...201525796039
draft genome sequences of five spore-forming food isolates of bacillus pumilus.here, we report the draft genome sequences of five food isolates of bacillus pumilus, a spore-forming gram-positive bacterium.201525767247
a novel intracellular nitrogen-fixing symbiosis made by ustilago maydis and bacillus spp.we observed that the maize pathogenic fungus ustilago maydis grew in nitrogen (n)-free media at a rate similar to that observed in media containing ammonium nitrate, suggesting that it was able to fix atmospheric n2 . because only prokaryotic organisms have the capacity to reduce n2 , we entertained the possibility that u. maydis was associated with an intracellular bacterium. the presence of nitrogenase in the fungus was analyzed by acetylene reduction, and capacity to fix n2 by use of (15) n2 ...201525754368
microbiological bioassay using bacillus pumilus to detect tetracycline in milk.the tetracyclines (tcs) are widely used in the treatment of several diseases of cattle and their residues may be present in milk. to control these residues it is necessary to have available inexpensive screening methods, user-friendly and capable of analysing a high number of samples. the purpose of this study was to design a bioassay of microbiological inhibition in microtiter plates with spores of bacillus pumilus to detect tcs at concentrations corresponding to the maximum residue limits (mrl ...201525720802
[characterization of β-1, 4-mannanase from bacillus pumilus and heterologous expression in lactobacillus casei].lactobacillus casei is widely used in food production and feed industry. the aim of this study was to construct the recombinant expression mannanase lb. casei.201527101700
[alkaline-adapted beta-mannanase of bacillus pumilus: gene heterologous expression and enzyme characterization].we expressed a novel alkaline-adapted beta-mannanase gene and characterized the enzyme for potential industrial applications.201526915226
improving the thermoactivity and thermostability of pectate lyase from bacillus pumilus for ramie degumming.thermostable alkaline pectate lyases can be potentially used for enzymatically degumming ramie in an environmentally sustainable manner and as an alternative to the currently used chemical-based ramie degumming processes. to assess its potential applications, pectate lyase from bacillus pumilus (atcc 7061) was cloned and expressed in escherichia coli. evolutionary strategies were applied to generate efficient ramie degumming enzymes. obtained from site-saturation mutagenesis and random mutagenes ...201525287558
probing c-terminal interactions of the pseudomonas stutzeri cyanide-degrading cynd protein.the cyanide dihydratases from bacillus pumilus and pseudomonas stutzeri share high amino acid sequence similarity throughout except for their highly divergent c-termini. however, deletion or exchange of the c-termini had different effects upon each enzyme. here we extended previous studies and investigated how the c-terminus affects the activity and stability of three nitrilases, the cyanide dihydratases from b. pumilus (cyndpum) and p. stutzeri (cyndstut) and the cyanide hydratase from neurospo ...201525549622
halotolerant pgprs prevent major shifts in indigenous microbial community structure under salinity stress.the resilience of soil microbial populations and processes to environmental perturbation is of increasing interest as alteration in rhizosphere microbial community dynamics impacts the combined functions of plant-microbe interactions. the present study was conducted to investigate the effect of inoculation with halotolerant rhizobacteria bacillus pumilus (str2), halomonas desiderata (str8), and exiguobacterium oxidotolerans (str36) on the indigenous root-associated microbial (bacterial and funga ...201525542205
single amino acid mutation alters thermostability of the alkaline protease from bacillus pumilus: thermodynamics and temperature dependence.dehairing alkaline protease (dhap) from bacillus pumilus ba06 has been demonstrated to have high catalytic efficiency and good thermostability, with potential application in leather processing. in order to get insights into its catalytic mechanism, two mutants with single amino acid substitution according to the homology modeling and multiple sequence alignment were characterized in thermodynamics of thermal denaturation and temperature dependence of substrate hydrolysis. the results showed that ...201525534779
action spectra for validation of pathogen disinfection in medium-pressure ultraviolet (uv) systems.ultraviolet (uv) reactors used for disinfecting water and wastewater must be validated and monitored over time. the validation process requires understanding the photochemical properties of the pathogens of concern and the challenge microorganisms used to represent them. specifically for polychromatic uv systems, the organisms' dose responses to uv light and their sensitivity across the uv spectrum must be known. this research measured the uv spectral sensitivity, called action spectra, of crypt ...201525506761
diversity and health status specific fluctuations of intrauterine microbial communities in postpartum dairy cows.for the interpretation of clinical findings of endometritis and the development of disease prevention and intervention strategies a better understanding of the dynamics and interactions within intrauterine bacterial communities in healthy and diseased cows is required. to gain deeper insights into fluctuations within the uterine microbiota, intrauterine samples were collected from 122 cows at the day of calving, days 3, 9, 15, 21 and 28 postpartum. a total of 2052 bacterial isolates were identif ...201525497238
treatment of textile effluent in a developed phytoreactor with immobilized bacterial augmentation and subsequent toxicity studies on etheostoma olmstedi fish.a static hydroponic bioreactor using nursery grown plants of pogonatherum crinitum along with immobilized bacillus pumilus cells was developed for the treatment of textile wastewater. independent reactors with plants and immobilized cells were also kept for performance and efficacy evaluation. the effluent samples characterized before and after their treatment showed that the plant-bacterial consortium reactor was more efficient than those of individual plant and bacterium reactors. cod, bod, ad ...201525464312
binding characteristics and synergistic effects of bacterial expansins on cellulosic and hemicellulosic substrates.expansins are non-catalytic proteins which loosen plant cell wall structure. in this study, binding kinetics and synergistic action of five bacterial expansins on cellulosic and hemicellulosic polysaccharides were studied. the expansins differed in binding capacity (bmax) and affinity (kd) for different substrates. a common pattern of binding efficiency (bmax/kd) was found among the expansins tested, in which efficiency was greatest for the phosphoric acid-swollen cellulose (pasc), then the hemi ...201525460993
isolation and characterisation of bacillus spp. antagonistic to vibrio parahaemolyticus for use as probiotics in aquaculture.acute gastroenteritis caused by pathogenic vibrio parahaemolyticus is one of the major factors affecting the development of aquaculture and the safety of seafood. using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and a common trend to control food-borne pathogenic bacteria. in this study, a total of 249 isolates were isolated from four types of seafood (litopenaeus vannamei, oratosquilla oratoria, mactra veneriformis and portunus trituberculatus) and ...201525737203
effects of actin-like proteins encoded by two bacillus pumilus phages on unstable lysogeny, revealed by genomic analysis.we characterized two newly isolated myoviruses, bp8p-c and bp8p-t, infecting the ginger rhizome rot disease pathogen bacillus pumilus gr8. the plaque of bp8p-t exhibited a clear center with a turbid rim, suggesting that bp8p-t could transform into latent phage. lysogeny assays showed that both the two phages could form latent states, while bp8p-t could form latent phage at a higher frequency and stability than bp8p-c. the genomes of bp8p-c and bp8p-t were 151,417 and 151,419 bp, respectively; bo ...201525344242
[structural properties and functional importance of metzincin metalloproteinases].here wediscuss known properties of metzincin metalloproteinases, their structure, physiological roles in the cell and potential medical uses. we also present results describing a novel extracellular metzincin metalloproteinase from bacillus pumilus with a unique combination of properties typical for both astacins and adamalysins.201525702412
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